RU2355170C1 - Medium for growth stimulation of green and grain crops with fungicide and antistress properties - Google Patents

Abstract

FIELD: agriculture.

SUBSTANCE: medium is a phyto-complex containing glycoside of phenol and polyphenol compounds, mono-and polysaccharides, maltol prepared from wood greens of siberian fir Abies sibirica, methyl the t-butyl ether treated in advance. The treated and dried wood greens of Siberian fir is extracted with boiling mixture of polar organic solvent (methanol, ethanol, isopropanol, acetone) with water (20-40% of water). The extract is concentrated. Yield of total extracted elements is 24-33%.

EFFECT: possibility to implement the function.

10 tbl, 7 ex, 1 dwg

Description

The invention relates to agriculture and can be used to stimulate the growth and development of cereal and vegetable crops, as well as to combat diseases of cereal plants (dusty and hard smut, root rot).

Substances that regulate plant growth began to be used in agriculture and horticulture already about 70 years ago. The discovery in the 20s of the last century of phytohormones that affect plant growth led first to the synthesis of analogues of natural phytohormones in laboratory conditions, and then to the creation of entire branches of the chemical industry that produce huge quantities of synthetic growth and plant protection regulators. The intensive use of synthetic growth regulators and plant protection in agriculture and horticulture in developed countries led to environmental pollution and soil depletion, which in turn necessitated the creation of tools that cause intensive multiplication of soil microorganisms-symbionts that enrich the soil to increase the yield of useful plants [ US 5002603, US 5085682, US 5125955]. As a result, the negative consequences of using synthetic plant growth and plant protection regulators forced researchers to look for alternative ways to increase the yield of agricultural plants that do not lead to environmental pollution. The most fruitful way turned out to be the use of renewable plant materials to create safe means for agrocenoses and humans to influence plants based on the principles of bioregulation.

Known growth-regulating complex and a preparation based on it [RU 2257059], obtained by alcohol extraction of echinacea purpurea. Air-dried plant material from the aerial parts of plants Echinacea purpurea is extracted with 90-96% ethanol with a ratio of ethanol and raw materials of 59-61: 1, respectively. The extract is acidified with salicylic acid to a pH of not more than 5.0, then purified by a polymer sorbent and concentrated by evaporation. The plant growth regulator contains chicory, chlorogenic and caffeic acid in a mass ratio of 79-81: 14-16: 4-6, respectively, and has an immunomodulating and anti-stress effect. This complex is used for processing plants and shrubs and for soaking seeds.

The disadvantages of this tool include the multi-stage production of it, the need to use salicylic acid as a substance that increases the yield of extractive substances, and expensive chromatographic purification of the extract to remove components that inhibit growth, as well as the limited resources of the original plant material for its production.

The prototype of the claimed tool is a complex of biologically active substances of phenolic and carbohydrate nature from woody green fir - the drug "Terpenol" [RU 2233868]. A method of obtaining the specified complex includes grinding the feedstock, extraction with an organic solvent and removing it by distillation. As raw materials, airborne dry woody greens are used. The raw material is extracted with a mixture of isopropanol: water (88:12), the extractant is distilled off, after which the extract is stratified into two layers at a temperature of 60-70 ° C for 48-72 hours. The lower aqueous organic layer is drained, cooled, washed with a non-polar solvent, the residual solvent is removed in vacuo, a viscous brown mass is obtained, soluble in organic solvents.

The drug "Terpenol" has several disadvantages. The disadvantages of the method of obtaining the drug is the long exposure (up to 3 days) of the extraction mixture at high temperature, the choice of the ratio of raw material to extractant not optimal for extraction processes is 1: (4-6). The drug is obtained in the form of a viscous mass, which is inconvenient during storage and transportation. The yield of the target product is low and is only 5-6%. For the analysis of the target product, the HPLC method with analytical wavelengths of 200 and 240 nm, which are not characteristic of the phenolic compounds of Siberian fir, is used. It is known that other wavelengths are used for the analysis of these substances, namely 280, 320 and 360 nm [Kosman V.M., Zenkevich I.G. Plant resources, 1997, issue 2, T.33, C.14-26]. As a result of this, it is impossible to assess the qualitative and quantitative composition of the phenolic component of the resulting complex of biologically active substances and thus standardize it.

The technical task of the present invention is the creation of a low-dose environmentally friendly natural remedy that allows to stimulate the growth and development of cereal and vegetable plants, as well as significantly reduce the damage to cereal plants by a number of diseases.

The problem is solved by a means containing as an active substance a complex of glycosides of phenolic and polyphenolic compounds, mono- and polysaccharides, maltol and obtained from dried woody green Siberian fir (Abies sibirica), pre-treated with methyl tert-butyl ether to separate non-polar compounds and the amount triterpenic acids (for example, in a known manner [RU 2108803]), drying the residue, followed by extraction with a mixture of polar organic solvents (methanol, ethanol, isopropanol, acetone) with water, the water content in the extractant is from 20 to 40%. This composition of water-organic extractants is due to the efficiency of the separation of phenolic compounds from plant materials. The extraction is carried out at the boil under reflux. The resulting extract is evaporated to a concentrate with a solids content of 5-8%. The solvents used can be used repeatedly. The yield of extractive substances is 24-33%. The extract is dried to a solid residue of dark brown color, suitable for long-term storage and transportation. The inventive tool is called by us ABISTIM.

Preliminary removal of non-polar compounds and the amount of triterpenic acids from the feedstock increases the recoverability of the target biologically active compounds by extraction with a mixture of polar solvent with water.

The tool contains as an active substance a complex comprising glycosides of phenolic and polyphenolic compounds, mono- and polysaccharides, maltol. To analyze the composition of the agent, the HPLC method was used, which is effectively used for the qualitative and quantitative analysis of phenolic compounds. The analysis of phenolic and polyphenolic compounds of the agent was performed on an Agilent 1100 liquid chromatograph with diode-matrix detection. Analysis conditions: column 4.6 × 150 mm filled with reverse phase sorbent ZORBAX Eclipse XDB-C8, 5 μm; gradient elution in the methanol system — 0.1% CF ₃ COOH (from 0 to 100% methanol in 20 minutes); eluent consumption - 0.8 ml / min. 280, 320 and 360 nm, which are characteristic of natural phenolic compounds, were selected as analytical wavelengths [Kosman V.M., Zenkevich I.G. Plant resources, 1997, issue 2, T.33, S.14-26; J. Agric. Food Chem, 2003, 51, 571-581].

According to the results of analysis by HPLC (see drawing) and IR spectroscopy, phenolic and polyphenolic compounds of ABISTIM are represented by glycosides of hydroxycinnamic and p-hydroxybenzoic acids, flavonoids, lignans. Hydroxycinnamic acids are represented by caffeic, ferulic, coumaric and 3,4-dimethoxycinnamic. Flavonoids are represented by dihydroquercetin, kempferol, quercetin and a group of catechins. Lignans - 3.4-divanyl tetrahydrofuran, matairesinol, etc. Monosaccharides are represented by glucose and fructose.

The biologically active properties of lignans, hydroxycinnamic acids, carbohydrates, maltol are known from the literature.

Lignans are hormone-like phytoestrogens contained in plants and participating in the mechanisms of plant protection from diseases and pests. In addition, they are associated with the regulation of plant growth [D'Abroska, B. et al., Phytochemistry, 58 (7), 1073-1081, 2001]. For example, in [Cutillo, Fr. et al., J. Agr. and Food Chem., 51 (21), 6165-6172, 2003] report that lignans such as laricirisinol and isolaricresinol can be used as natural herbicides. Matairezinol exhibits antibacterial activity [Yang, M. et. al., Pharmazie; 59; 12; 2004; 972-976]. The antiviral and bactericidal properties of lignans have been reported in the literature [Adlercreutz, H. 1991. Diet and Sex Hormone Metabolizm. In: Rowland, I.R. (ed.) Nutrition, Toxicity and Cancer. CRC Press. P.137-195].

Hydroxycinnamic acids are described in the literature as fungicidal, antibacterial agents (for example, preparations Domotsvet R, Zirkon R [List of pesticides and agrochemicals approved for use in the Russian Federation. 2007, Moscow, 2007]). High activity in the fight against insect pests, pathogenic fungi and bacteria showed a product containing hydroxycinnamic acid, aldehyde or ether [US 5839224].

Carbohydrates increase the frost resistance of plant tissues and cells, allowing them to tolerate temperatures below 0 ° C [Kramer PD, Kozlovsky GG The physiology of woody plants, Moscow, 1998], and also provide cells with additional nutrition in the presence of a growth stimulator [JP 02234809].

Maltol has bactericidal and fungicidal properties [RU 2195948].

Since the isolation of each biologically active compound in pure form from plant materials is difficult (or simply impossible) and economically unjustified, moreover, as already noted by many researchers, the biological activity of mixtures is often higher than the biological activity of its individual components, the invention claims a tool that represents a mixture of biologically active components obtained in a specific way.

The inventive tool has the following advantages:

- the composition of the obtained biologically active agent ABISTIM includes glycosides of phenolic and polyphenolic compounds, mono- and polysaccharides, maltol, which makes it possible to use the resulting phytocomplex of Siberian fir in agriculture as a low-dose stimulating and protective action for plant growing;

- phenolic and polyphenolic compounds that make up the active complex of the agent are in the form of glycosides, which significantly increases their bioavailability;

- carbohydrates of the active complex of the claimed funds are nutritional and skeletal materials of plant cells and tissues;

- due to the bactericidal properties of phenolic compounds, the presence of mono- and polysaccharides in it does not lead to the growth of microflora during storage;

- the method of producing ABISTIM allows you to get an additional 25% of the amount of polar extractive substances due to the extraction of mixtures of polar solvents and water of woody green Siberian fir, pre-treated with methyl tert-butyl ether to remove non-polar compounds and the amount of triterpene acids (preliminary removal of non-polar compounds and the amount of triterpene acids from raw materials increases the recoverability of the target biologically active compounds);

- receipt of the claimed funds in the form of a solid product ensures the preservation of its consumer qualities for a long time and simplifies the conditions for its transportation;

- the method of obtaining the inventive tool allows you to use the organic component of the extracting mixture (alcohols or acetone) repeatedly;

- the process of obtaining the claimed funds is short (compared with the prototype, the total time to obtain the active complex is reduced by 2-3 times);

- the resources of the feedstock for obtaining the claimed funds are large because the area of growth of Siberian fir is extensive, moreover, the use of woody greens can solve the problem of the integrated use of conifers in their processing.

The method of obtaining funds is illustrated by the following examples:

Example A.

300 ml of methyl tert-butyl ether are added to 42 g of chopped wood green fir, refluxed for 1 hour, the extract is removed, the feed is washed with methyl tert-butyl ether and dried. To air-dry raw materials freed from the fraction of non-polar compounds and triterpene acids, 400 ml of a water-ethanol mixture with a volume ratio of 3: 7 are added. It is refluxed for 1.5 hours. The extract is drained hot. The raw material remaining in the flask is extracted an additional two times with 200 ml portions of a water-ethanol mixture. The aqueous ethanol extract combined after three times extraction was concentrated by evaporation on a rotary evaporator to 170 ml. The extract concentrate is a turbid brown-green color, dry weight - 6.6%. The output of the amount of extractive substances 27%.

The extract concentrate is dried in an oven at a temperature of 70-80 ° C to obtain a dark brown solid residue.

Example B.

To 12 g of chopped green wood fir, add 80 ml of methyl tert-butyl ether, refluxed for 1 h, the extract is removed, the feed is washed with methyl tert-butyl ether and dried. To air-dry raw materials, freed from the fraction of non-polar compounds and triterpene acids, add 80 ml of water-ethanol mixture with a volume ratio of 3: 7. It is boiled once under reflux for 1.5 hours. The extract is drained hot. The extract was concentrated by evaporation on a rotary evaporator to 37 ml. Dry weight - 8.0%. The extract concentrate is a turbid brown-green color. The output of the sum of extractive substances 25%.

Example B.

To 6 g of chopped wood green fir, 300 ml of methyl tert-butyl ether are added, refluxed for 1 hour, the extract is removed, the feed is washed with methyl tert-butyl ether and dried. To air-dry raw materials freed from the fraction of non-polar compounds and triterpene acids, add 50 ml of water-isopropanol mixture with a volume ratio of 3: 7. It is refluxed for 1.5 hours. The extract is drained hot. The raw material remaining in the flask is re-extracted with 50 ml of water-isopropanol mixture. The water-isopropanol extract combined after twofold extraction was concentrated by evaporation on a rotary evaporator to 35 ml. Dry weight - 5.4%. The extract concentrate is a turbid brown-green color. The output of the sum of extractive substances 33%.

Example G.

To 5.6 g of chopped wood green fir, 60 ml of methyl tert-butyl ether are added, refluxed for 1 hour, the extract is removed, the feed is washed with methyl tert-butyl ether and dried. To air-dry raw materials freed from the fraction of non-polar compounds and triterpene acids, add 50 ml of water-acetone mixture with a volume ratio of 3: 7. It is refluxed for 1.5 hours. The extract is drained hot. The raw material remaining in the flask is extracted again with 40 ml of an aqueous-acetone mixture. The combined extract was concentrated by evaporation on a rotary evaporator to 17 ml. The extract concentrate is a turbid brown-green color, dry weight - 7.5%. The output of the amount of extractives is 24%.

Example D.

To 4.5 g of chopped woody fir green, 60 ml of methyl tert-butyl ether are added, refluxed for 1 hour, the extract is removed, the feed is washed with methyl tert-butyl ether and dried. To air-dry raw materials, freed from the fraction of non-polar compounds and triterpene acids, add 50 ml of water-methanol mixture with a volume ratio of 3: 7. It is refluxed for 1.5 hours. The extract is drained hot. The raw material remaining in the flask is extracted again with 40 ml of a water-methanol mixture. The combined extract was concentrated by evaporation on a rotary evaporator to 14 ml. The extract concentrate is a turbid brown-green color, dry weight - 7.6%. The output of the amount of extractives is 24%.

An analysis of the concentrates of the obtained extracts to determine the glycoside content of polyphenolic compounds and monosaccharides is performed by HPLC. Analysis of the concentrates of the obtained extracts for the content of polysaccharides is carried out by the gravimetric method for precipitation with alcohol. The ratio of glycosides of phenolic and polyphenolic compounds, monosaccharides, polysaccharides and maltol in the extract is approximately 1: 1: 1: 0.1.

The drug on the basis of the claimed means ABISTIM is obtained by mixing the extract concentrate (in terms of dry weight), liquid soap (for example, TU OP 2381-080-00279611-98) and water in a ratio of 1: 2: 17 (5% by dry concentrate).

Field tests of ABISTIM were carried out on vegetable and zoned cereal crops, according to agrotechnical dates, on the phytopathological site of the immunity laboratory and on the fields of the vegetable and potato department of GNU SibNIIRS SB Rosselkhozakademii in 2006, 2007.

Stimulation of plants in the early stages of organogenesis and their protection against diseases with ABISTIM is carried out in the following way: for several days or on the day of sowing, pre-sowing treatment of seeds of grain crops (wheat, barley, oats) with the biologically active agent ABISTIM (5% aqueous emulsion of the agent before diluted with water to the required rate of working solution). The consumption rate of the drug is 100 ml / t, the flow rate of the working solution is 10 l / t.

To assess the effectiveness of the drug ABISTIM in the experiments used seeds of varieties of cereals that are unstable to smut diseases. Infected with a population of smut fungi (dusty, hard). During artificial infection of wheat with dusty smut (in the flowering phase), a population of 5, 8, 10, 12, 21, 23, 25, 63, 64 races was used, where 63, 64 races were isolated in Siberia and were first registered in our country ( year of deposit 2003).

In the experiments used control-1 - seeds (infected with smut pathogen) of various varieties of crops without treatment with ABISTIM (water); control-2 - healthy (without infection) seeds of varieties, without treatment with drugs (water).

Sowing was carried out with SKS-7 cassette seeder of 100 grains per 1 linear meter, in three repetitions, taking into account the recommended regional agricultural technology. The counts and observations were carried out by generally accepted methods accredited by research institutes in the conditions of laboratory field experiments. The test results of the inventive means ABISTIM are given in tables 1-3.

Tables 1-3 show the effectiveness of the claimed funds on crops against pathogens of dusty, smut and root rot.

The research results indicate a high (100%) biological effectiveness of the claimed funds against hard smut on barley and reducing dusty smut of wheat, barley, oats to 69.4%. A decrease in root rot to the threshold level of severity was noted:

on wheat - 75.8%,

on barley - 42.5%,

on oats - 88.6% compared with control-1.

As a result of using the inventive product, an increase was obtained in the yield of spring wheat to 5.9 c / ha, barley to 24.6 c / ha, oats to 14.0 c / ha compared with the infected control.

In tables 4-10 shows the effectiveness of the claimed funds ABISTIM on vegetable crops.

Example 1. Tests on the cucumber.

Potted seedlings were grown in the greenhouse for 30-35 days. Planting cucumber seedlings is two-line (90 × 50 × 40 cm) in the phase of 5-6 leaves on 09.06.2007, in a 3-fold repetition, in a film greenhouse. Foliar treatment of plants with a solution of the drug was carried out 1 time on 06/23/2007 in the phase of the onset of flowering. The consumption rate of 5% of the drug is 5 ml per 1 liter of water. Daily care of plants and watering with a nutrient solution of Chesnokov-Bazyrin. The formation and garter of plants after 3-4 days. The harvest is reusable, 3 times a week. The results are shown in table 4.

The results indicate the influence of ABISTIM on the increase in early and total productivity by 1.3 and 1.2 times, an increase in the yield of standard fruits by 1.2 times in comparison with the control. There was a decrease in disease damage, where the biological efficiency for root rot is 21%, for peronosporosis - 28.6%, for powdery mildew - 100%.

Example 2. Tests on cauliflower.

Sowing cabbage seeds in boxes 03/05/2007, picking seedlings in pots under a film shelter 05/14/2007. Planting cabbage in open ground 06/11/2007, planting pattern 0.5 × 0.5 m ² , 3-fold repetition. Conducted a single foliar treatment of plants at the rate of 5 ml of 5% of the drug per 1 liter of water. Plant care: loosening 4 times, inter-row processing 2 times, top dressing, irrigation, crop accounting 12.08.2007 - 10.09.2007. The results are shown in table.5.

From table 5 it is seen that a single treatment with ABISTIM cauliflower plants in the phase of 6-7 leaves increased both the early and the total yield 1.9 and 1.4 times, respectively, the weight of the head is 1.4 times in comparison with the control. Productivity after one treatment with Novosil is below control.

Example 3. Tests on white cabbage.

Sowing cabbage seeds in boxes 04/23/2006, picking seedlings under a film shelter 05/15/2006, planting cabbage in open ground 06/01/2006, planting pattern 0.7 × 0.7 × 4.9 m ² , 3-fold repetition. Foliar treatment of plants was carried out twice: the 1st treatment in the phase of 2-4 leaves (05/30/2006), the 2nd in the phase of 6-7 leaves (06/23/2006) at the rate of 3 ml of 5% preparation per 1.5 liters of water. Plant care: loosening 3 times, inter-row processing 2 times, top dressing, watering, crop accounting 09/27/2006. The results are presented in table 6.

There was an increase in productivity by 100 kg / ha - 1.2 times, an increase in the density and mass of heads of cabbage 1.1 and 1.3 times, respectively, compared with the control.

Example 4. Tests on spring garlic.

The plot area is 2.15 m ² , the repetition is 3-fold. Planting pattern 70x6 cm, 50 teeth planted in a row. Planting of garlic held 05/07/2006.

According to the experimental design, the plants were sprayed with solutions in the phases of 3, 5 leaves (05/30/2006) and mass formation of leaves (06/23/2006). The consumption rate of 5% of the drug is 2 ml per 1 liter of water. During the growing season, plants were maintained (weeding, loosening, watering, and feeding with micro and macro fertilizers). Harvesting made on 08.25.2006.

The results are presented in table 7. The treatment of plants with ABISTIM increased the yield of garlic by 1.2 times, increased the yield of bulbs on the market by 1.3 times in comparison with the control.

Example 5. Tests on winter garlic.

The plot area is 4.2 m ² , the repetition is 3-fold. Planting scheme 70 × 4 cm. Planting of garlic - 09/25/2006. According to the experimental design, plants were sprayed with solutions in phases of 3 to 5 leaves (05/10/2007); mass formation of leaves and bulbs on June 23, 2007. The consumption rate of 5% of the drug is 2 ml per 1 liter of water. The results are presented in table.8.

From table 8 it can be seen that processing with ABISTIM increased productivity by 3.8 centners - 1.1 times and increased the yield of bulbs on a 1.2-fold basis compared with the control.

Example 6. Tests on eggplant.

Potted seedlings were grown in the greenhouse for 60 days. Planting eggplant seedlings under a film shelter 06/13/2007. Repeat 3-fold. A single spraying of plants was carried out on 06/23/2007 at the rate of 5 ml of 5% preparation per 1 liter of water. Plant care: garter, loosening, top dressing, watering, crop accounting as the fruits ripen.

The results are presented in table 9.

Processing with ABISTIM increased the early eggplant yield by 0.4 kg / m ² (1.3 times) and the total by 1.8 kg / m ² (1.4 times), increased the number and weight of marketable fruits by 1.2 and 1.3 times, respectively, compared to the control.

Example 7. Tests on pepper.

Potted seedlings were grown in the greenhouse for 60 days. Planting pepper seedlings under a film shelter 06/13/2007. Repeat 3-fold. A single foliar treatment of plants was done on June 23, 2007 at the rate of 5 ml of 5% preparation per 1 liter of water. Plant care: garter, loosening, top dressing, watering, crop accounting as the fruits ripen. The results are presented in table 10.

The results of table 10 indicate a significant effect of ABISTIM on the formation of an early 2.6-fold and 2.2-fold total crop. The increase in the total yield consists of a 1.3-fold increase in the weight of one fruit and a 1.7-fold increase in the number of fruits in comparison with the control.

Table 1. The effect of pre-sowing treatment with ABISTIM on diseases and productivity of the spring apple of the Skala variety (2006, 2007) Option The defeat of the disease,% Number of healthy ears, pcs Mass of 1000 grains, g Productivity, t / ha Increase smut root rot c / ha % PG BE IRB BE 2006 year Abistim 1.1 69.4 8.6 1.2 113.7 37.4 30.0 5.9 124.5 Control (infected with PG) 3.6 - 8.7 - 100.7 33.0 24.1 - 100.0 2007 year Abistim 0.8 65.2 17.1 75.8 122.5 29.0 27.4 4.9 121.8 Control (infected with PG) 2.3 - 41.3 - 101.3 27.0 22.5 - 100.0 Note: PG - smut smut; BE - biological effectiveness;
RBI is a disease development index.

Table 2. The effect of presowing treatment with ABISTIM on diseases and productivity of barley varieties Simcho, 2006 Option The defeat of the disease,% Number of healthy ears, pcs Mass of grain Increase smut root rot from the plot, t / ha 1000 grains, g from the ear, g with a plant, g c / ha % PG BE TG BE IRB BE Abistim 0.9 10 0 one hundred 7.7 42.5 185.3 62.0 54.8 0.7 2.5 24.6 165.8 The control 1.0 - 15.4 - 13.4 - 128.5 37.4 48.5 0.6 1.7 - 100.0 Note: PG - smut smut; BE - biological effectiveness; TG - smut;
RBI is a disease development index.

Table 3. The effect of pre-sowing treatment with ABISTIM on diseases and productivity of Zenith oats (2006,2007) Option The defeat of the disease,% Quantity, pcs Mass of grain Increase smut root rot healthy panicles grains in a panicle from the plot, t / ha from panicle, g with a plant, g c / ha % PG BE IRB BE 2006 year Abistim 8.6 67.9 0.8 88.6 71.3 30.0 44.4 1.3 3.8 14.0 146.1 Control (infected with PG) 26.8 - 7.0 - 56.7 24.7 30.4 1.1 2.5 - 100.0 2007 year Abistim 16.1 37.8 3.4 69.9 84.0 51.0 69.5 2.1 5.9 11.6 120.0 Control (infected with PG) 25.9 - 11.3 - 60.0 43.8 57.9 1.9 5.3 - 100.0 Control (uninfected GHG) 1.0 96.1 2.1 81.4 109.5 49.2 88.7 1.6 3.5 30.8 153.2 Note: PG - smut smut; BE - biological effectiveness;
RBI is a disease development index.

Table 4. The influence of ABISTIM on the performance of cucumber Jade F ₁ , 2007 Experience Options Productivity Early harvest to total,% The mass of marketable fruit, g Number of fruits on 1 plant, pcs. early (6.07-16.07) general (6.07-13.08) kg / m ² % to control kg / m ² % to control Abistim 2.8 133.3 8.2 117.1 34.1 65 fifty Novosil 2.4 114.3 7.6 108.6 31.6 62 49 The control 2.1 one hundred 7.0 one hundred 30.0 65 43 Table 5.
The influence of ABISTIM on indicators of the yield of cauliflower Boldo F ₁ , 2007 Experience Options Productivity Early harvest to total,% Weight of head of cabbage early common kg % to control kg / m ² % to control kg / m ² % to control Abistim 1.3 185.7 4.6 135.3 28.3 1.15 135.3 Novosil 0.56 80.0 2.84 83.5 19.7 0.71 83.5 The control 0.7 100.0 3.4 100.0 20.6 0.85 100.0

Table 6. The influence of ABISTIM on the productivity indicators of white cabbage of the Blizzard variety (spraying of crops on 05/30/06; 06/23/06), 2006 Experience Options Plant height, cm Outlet diameter, cm The density of the head, kg / dm ³ The length of the poker, cm Weight kg Productivity from 1 m ² head out c / ha % to control increase, t / ha Abistim 28 63/60 0.9 6 5.5 2.8 550 122.2 one hundred The control 28 62/59 0.8 7 4.5 2.2 450 100.0 -

Table 7. The effect of spraying with ABISTIM on the yield of spring garlic (spraying of crops on 05/30/2006; 06/23/2006), 2006 Experience Options Commodity Non-commodity Single tooth Total harvest Quantity, pcs / m ² Weight Quantity, pcs / m ² Weight Quantity, pcs / m ² g / m ² % to control g / m ² The increase to control, t / ha One onion, g g / m ² % to control One onion, g g / m ² % to control Abistim 8 18.0 143 125.4 eleven 8.9 98 140 four 12 57.1 253 4.8 The control 6 19.0 114 100.0 10 7.0 70 one hundred 6 21 100.0 205 - Table 8. The effect of spraying with ABISTIM on the yield of winter garlic of the Alcor variety (spraying of crops on May 10, 2007; June 23, 2007), 2007 Experience Options Commodity Non-commodity Single tooth Total harvest Quantity, pcs / m ² Weight Quantity, pcs / m Weight Quantity, pcs / m ² g / m ² % to control g / m ² The increase to control, t / ha One onion, g g / m ² % to control One onion, g g / m ² % to control Abistim 10 38.9 389 120.1 7 17 119 94.4 3 4.5 75 549 3.8 The control 9 36.0 324 one hundred 6 21 126 one hundred four 6.0 one hundred 511 -

Table 9. Influence of ABISTIM on the productivity of eggplant variety, Skorospeliy, 2007 Experience Options Productivity Early harvest to total,% The mass of marketable fruit, g Number of fruits on 1 plant, pcs early common kg / m ² % to control kg / m ² % to control Abistim 2.0 125.0 6.3 140.0 31.7 143 eleven Novosil 1.52 95.0 3.08 68.4 49.4 128 6 The control 1.6 100.0 4.5 100.0 35.6 125 9 Table 10. The influence of ABISTIM on the productivity of Gagashary pepper, 2007 Experience Options Productivity Early harvest to total,% The mass of marketable fruit, g Number of fruits on 1 plant, pcs. early common kg / m ² % to control kg / m ² % to control Abistim 1.8 257.1 3.9 216.7 46.2 98 10 Novosil 1.3 185.7 2.7 150.0 48.1 84 8 The control 0.7 100.0 1.8 100.0 38.9 75 6

Claims (1)

Means for stimulating the growth of vegetables and grain crops with fungicidal and anti-stress properties, containing a complex of biologically active glycosides of phenolic and polyphenolic compounds, mono- and polysaccharides, maltol, obtained from Abies sibirica fir wood by extraction with methyl tert-butyl ether, separation of this extract , drying the residue and its subsequent extraction by boiling in a mixture of polar organic solvents (methanol, ethanol, isopropanol, acetone) with water at a water content of 20-40%.

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