MD902Z - Process for treatment of spring wheat - Google Patents
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- MD902Z MD902Z MDS20140116A MDS20140116A MD902Z MD 902 Z MD902 Z MD 902Z MD S20140116 A MDS20140116 A MD S20140116A MD S20140116 A MDS20140116 A MD S20140116A MD 902 Z MD902 Z MD 902Z
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- 241000209140 Triticum Species 0.000 title claims abstract description 19
- 235000021307 Triticum Nutrition 0.000 title claims abstract description 11
- 238000000034 method Methods 0.000 title claims abstract description 9
- 239000000243 solution Substances 0.000 claims abstract description 12
- 241000196324 Embryophyta Species 0.000 claims abstract description 7
- 241000983759 Linaria genistifolia Species 0.000 claims abstract description 6
- 229930182489 iridoid glycoside Natural products 0.000 claims abstract description 6
- 150000008145 iridoid glycosides Chemical class 0.000 claims abstract description 6
- 239000007864 aqueous solution Substances 0.000 claims abstract description 4
- 238000009835 boiling Methods 0.000 claims abstract description 4
- 239000000284 extract Substances 0.000 claims abstract description 4
- 238000000605 extraction Methods 0.000 claims abstract description 3
- 241000223218 Fusarium Species 0.000 abstract description 7
- 241000520648 Pyrenophora teres Species 0.000 abstract description 7
- UHPMCKVQTMMPCG-UHFFFAOYSA-N 5,8-dihydroxy-2-methoxy-6-methyl-7-(2-oxopropyl)naphthalene-1,4-dione Chemical compound CC1=C(CC(C)=O)C(O)=C2C(=O)C(OC)=CC(=O)C2=C1O UHPMCKVQTMMPCG-UHFFFAOYSA-N 0.000 abstract description 6
- GBMDVOWEEQVZKZ-UHFFFAOYSA-N methanol;hydrate Chemical compound O.OC GBMDVOWEEQVZKZ-UHFFFAOYSA-N 0.000 abstract 1
- 238000011161 development Methods 0.000 description 17
- 230000018109 developmental process Effects 0.000 description 17
- 201000010099 disease Diseases 0.000 description 9
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 9
- 229930182470 glycoside Natural products 0.000 description 9
- 150000002338 glycosides Chemical class 0.000 description 9
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 6
- 244000052769 pathogen Species 0.000 description 6
- 238000002360 preparation method Methods 0.000 description 4
- 230000002538 fungal effect Effects 0.000 description 3
- 230000000855 fungicidal effect Effects 0.000 description 3
- 239000008267 milk Substances 0.000 description 3
- 210000004080 milk Anatomy 0.000 description 3
- 235000013336 milk Nutrition 0.000 description 3
- 230000001717 pathogenic effect Effects 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 241000233866 Fungi Species 0.000 description 2
- 241000227653 Lycopersicon Species 0.000 description 2
- 235000007688 Lycopersicon esculentum Nutrition 0.000 description 2
- 208000031888 Mycoses Diseases 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 235000013339 cereals Nutrition 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 244000053095 fungal pathogen Species 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 239000010902 straw Substances 0.000 description 2
- 238000005292 vacuum distillation Methods 0.000 description 2
- 238000001644 13C nuclear magnetic resonance spectroscopy Methods 0.000 description 1
- 238000005160 1H NMR spectroscopy Methods 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- 208000004770 Fusariosis Diseases 0.000 description 1
- DRWIVSAUXCDFQK-UHFFFAOYSA-N Linarioside Natural products C12C(C)(O)C(Cl)C(O)C2(O)C=COC1OC1OC(CO)C(O)C(O)C1O DRWIVSAUXCDFQK-UHFFFAOYSA-N 0.000 description 1
- 241000013557 Plantaginaceae Species 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 240000003768 Solanum lycopersicum Species 0.000 description 1
- 235000002560 Solanum lycopersicum Nutrition 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 238000009924 canning Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000000411 inducer Substances 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 238000009533 lab test Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- XELZGAJCZANUQH-UHFFFAOYSA-N methyl 1-acetylthieno[3,2-c]pyrazole-5-carboxylate Chemical compound CC(=O)N1N=CC2=C1C=C(C(=O)OC)S2 XELZGAJCZANUQH-UHFFFAOYSA-N 0.000 description 1
- 230000003032 phytopathogenic effect Effects 0.000 description 1
- 239000001397 quillaja saponaria molina bark Substances 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000005070 ripening Effects 0.000 description 1
- 229930182490 saponin Natural products 0.000 description 1
- 150000007949 saponins Chemical class 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000000741 silica gel Substances 0.000 description 1
- 229910002027 silica gel Inorganic materials 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
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- 238000010561 standard procedure Methods 0.000 description 1
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- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 description 1
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 239000012224 working solution Substances 0.000 description 1
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Abstract
Изобретение относится к сельскому хозяйству, в частности к способу обработки яровой пшеницы.Способ, согласно изобретению, включает обработку растений пшеницы в фазе кущения водным раствором, содержащим 5x10-4 % суммарного экстракта иридоидных гликозидов из растений Linaria genistifolia L. Mill, полученного путем экстракции воднометанольным раствором при кипячении, с общим расходом 250 л/га.Результат состоит в повышении устойчивости яровой пшеницы к фитопатогенам Pyrenophora teres и Fusarium spp.The invention relates to agriculture, in particular to a method for processing spring wheat. The method according to the invention includes the treatment of wheat plants in the tillering phase with an aqueous solution containing 5x10-4% of the total extract of iridoid glycosides from Linaria genistifolia L. Mill plants obtained by extraction with water methanol solution at boiling, with a total flow rate of 250 l / ha. The result is to increase the resistance of spring wheat to the pathogens Pyrenophora teres and Fusarium spp.
Description
Invenţia se referă la agricultură, şi anume la un procedeu de tratare a grâului de primăvară. The invention relates to agriculture, namely to a process for treating spring wheat.
Este cunoscut procedeul de sporire a rezistenţei grâului faţă de patogenii bolilor foliare prin tratarea plantelor cu soluţie de tomatozidă [1]. The process of increasing wheat resistance to foliar disease pathogens by treating plants with tomatozide solution is known [1].
Dezavantajul procedeului dat constă în efectul insuficient al tratării plantelor de grâu cu soluţii de tomatozidă, totodată tehnologia de obţinere a preparatului de tomatozidă este costisitoare din punct de vedere economic, implicând costuri ridicate ale materiei prime - seminţelor de tomate. The disadvantage of this process is the insufficient effect of treating wheat plants with tomatozide solutions, while the technology for obtaining the tomatozide preparation is economically expensive, involving high costs of the raw material - tomato seeds.
Problema pe care o rezolvă invenţia este consolidarea rezistenţei grâului de primăvară faţă de bolile fungice, reducerea răspândirii şi a gradului de dezvoltare a lor. The problem solved by the invention is to strengthen the resistance of spring wheat to fungal diseases, reducing their spread and degree of development.
Procedeul de tratare a grâului de primăvară, conform invenţiei, include tratarea plantelor de grâu în faza de înfrăţire cu o soluţie apoasă ce conţine 5x10-4 % extract sumar de glicozide iridoidice din plante de Linaria genistifolia L. Mill, obţinut prin extragere cu soluţie hidrometanolică la fierbere, cu un consum total de 250 L/ha. The spring wheat treatment process, according to the invention, includes treating wheat plants in the twinning phase with an aqueous solution containing 5x10-4 % summary extract of iridoid glycosides from Linaria genistifolia L. Mill plants, obtained by extraction with boiling hydromethanolic solution, with a total consumption of 250 L/ha.
Suma de glicozide (genistifoliozide) se obţine din plante sălbatice indigene Linaria genistifolia L. Mill, fam. Scrophulariaceae. The sum of glycosides (genistifoliosides) is obtained from the indigenous wild plant Linaria genistifolia L. Mill, family Scrophulariaceae.
Rezultatul invenţiei constă în mărirea rezistenţei grâului de primăvară faţă de agenţii fitopatogeni Pyrenophora teres Drechsler şi Fusarium spp., ce contribuie la reducerea răspândirii şi a gradului de dezvoltare a bolilor fungice. The result of the invention consists in increasing the resistance of spring wheat to the phytopathogenic agents Pyrenophora teres Drechsler and Fusarium spp., which contributes to reducing the spread and degree of development of fungal diseases.
Glicozidele iridoidice sumare din Linaria genistifolia L. Mill s-au obţinut în modul următor: masa vegetală a plantei (partea aeriană) uscate, în cantitate de 300 g, a fost mărunţită şi extrasă cu soluţie hidrometanolică de 60% de 3 ori prin fierbere. Extractele au fost unite şi concentrate prin distilare în vid, iar reziduul apos a fost trecut prin coloană cu Sephadex LH-20. Coloana a fost eluată cu apă, apoi cu soluţie hidrometanolică de 10%, după care cu metanol. Eluatele cu soluţie hidrometanolică de 10% şi metanol au fost unite şi concentrate prin distilare în vid până la uscat. În reziduul uscat (4,6 g) au fost detectate cu ajutorul cromatografiei în strat subţire de silicagel 4 glicozide iridoidice, denumite genistifoliozide 1-4. Conform cercetărilor ulterioare, după separarea componentelor amestecului prin metode cromatografice, cu ajutorul procedeelor fizico-chimice, spectroscopiei H1 şi 13C RMN, genistifoliozidele 1-4 au fost identificate prin comparare cu datele bibliografice după cum urmează: 1 - antirinozida; 2 - linariozida; 3 - 5-O-alozilantirinozida; 4 - 6-b-hidroxiantirida. De asemenea, s-a stabilit că 5-O-alozilantirinozida este componenta majoră în amestecul sumar de glicozide iridoidice. The total iridoid glycosides from Linaria genistifolia L. Mill were obtained as follows: the dried plant mass (aerial part), in an amount of 300 g, was ground and extracted with 60% hydromethanol solution 3 times by boiling. The extracts were combined and concentrated by vacuum distillation, and the aqueous residue was passed through a Sephadex LH-20 column. The column was eluted with water, then with 10% hydromethanol solution, and then with methanol. The eluates with 10% hydromethanol solution and methanol were combined and concentrated by vacuum distillation to dryness. In the dry residue (4.6 g) 4 iridoid glycosides, called genistifoliosides 1-4, were detected using silica gel thin layer chromatography. According to further research, after separation of the components of the mixture by chromatographic methods, using physicochemical procedures, H1 and 13C NMR spectroscopy, genistifoliosides 1-4 were identified by comparison with bibliographic data as follows: 1 - antirinoside; 2 - linarioside; 3 - 5-O-allosylantirinoside; 4 - 6-b-hydroxyantirinoside. It was also established that 5-O-allosylantirinoside is the major component in the total mixture of iridoid glycosides.
Exemplu de realizare a invenţiei Example of embodiment of the invention
Cercetările au fost efectuate în condiţii de laborator şi de câmp pe plante de grâu de primăvară, soiul Rostan. The research was conducted under laboratory and field conditions on spring wheat plants, the Rostan variety.
În condiţii de laborator glicozidele sumare din Linaria genistifolia (genistifoliozide) în intervalul concentraţiilor 10-4…5х10-4% au fost testate la activitatea fungicidă faţă de două specii de fungi: Pyrenophora teres Drechsler - agent patogen de pătare reticulară a frunzelor şi Fusarium spp. - patogen de fuzarioză, populaţiile cărora au fost izolate din plante de grâu infectate. Pentru comparaţie a fost utilizată tomatozida {3-O-β-D-glucopiranozil (1→2)-O-β-D-glucopiranozil (1→4)-O-β-D-galactopiranozid-[(25S)-5α-furostan-3β,22α,26-triol-26-O-β-D-glucopiranozid}, obţinut din seminţe de Solanum lycopersicum Mill. [Hiroji SATO, Sadao SAKAMURA. Bitter of Tomato Seeds Isolation and Structure of a New Furostanol Saponin. Agr. Biol. Chem., 37 (2), 1973, p. 225-231]. In laboratory conditions, the total glycosides from Linaria genistifolia (genistifoliosides) in the concentration range of 10-4…5х10-4% were tested for fungicidal activity against two species of fungi: Pyrenophora teres Drechsler - pathogen of reticulate leaf spot and Fusarium spp. - pathogen of fusariosis, the populations of which were isolated from infected wheat plants. For comparison, tomatoside {3-O-β-D-glucopyranosyl (1→2)-O-β-D-glucopyranosyl (1→4)-O-β-D-galactopyranoside-[(25S)-5α-furostane-3β,22α,26-triol-26-O-β-D-glucopyranoside}, obtained from seeds of Solanum lycopersicum Mill. [Hiroji SATO, Sadao SAKAMURA. Bitter of Tomato Seeds Isolation and Structure of a New Furostanol Saponin. Agr. Biol. Chem., 37 (2), 1973, p. 225-231].
În acest scop, în cutii Petri sterile cu diametrul de 15 mm s-au turnat câte 15 ml de mediu nutritiv cu soluţii de glicozide, sterilizat anterior. După solidificarea mediului în centrul cutiei pe suprafaţa agarului s-au adăugat sporii fungilor. Timp de 10 zile de cultivare în luminostat s-a măsurat diametrul zonelor de creştere. Fiecare experiment a fost efectuat în 4 replici. În calitate de control au servit cutiile cu mediu nutritiv fără glicozide. Activitatea fungicidă a fost determinată prin măsurarea modificării diametrului zonei de creştere a fungilor patogeni sus-numiţi. For this purpose, 15 ml of previously sterilized nutrient medium with glycoside solutions were poured into sterile Petri dishes with a diameter of 15 mm. After the medium solidified in the center of the dish on the agar surface, fungal spores were added. During 10 days of cultivation in the light, the diameter of the growth zones was measured. Each experiment was performed in 4 replicates. The dishes with nutrient medium without glycosides served as a control. The fungicidal activity was determined by measuring the change in the diameter of the growth zone of the above-mentioned pathogenic fungi.
Rezultatele cercetărilor au arătat că genistifoliozidele în ambele concentraţii au inhibat dezvoltarea miceliului fungic (tab. 1, fig. 1), în plus creşterea concentraţiei a sporit semnificativ eficacitatea preparatului. Astfel, diametrul zonelor de dezvoltare a Fusarium spp. în cutiile Petri cu preparatul genistifoliozidă cu concentraţia de 5х10-4% a fost mai mic cu 54,5% în comparaţie cu tomatozida şi cu 92,7% faţă de varianta de control. Diametrul zonei de dezvoltare a patogenului de pătare reticulară a frunzelor Pyrenophora teres, în urma adăugării în mediul de cultivare a preparatului testat în concentraţie de 5х10-4%, a fost mai mic cu 67,5% decât în varianta cu tomatozidă (tab. 1, fig. 2). The results of the research showed that genistifoliosides in both concentrations inhibited the development of fungal mycelium (tab. 1, fig. 1), in addition, increasing the concentration significantly increased the effectiveness of the preparation. Thus, the diameter of the zones of development of Fusarium spp. in Petri dishes with the genistifoliosides preparation at a concentration of 5х10-4% was 54.5% smaller than with tomatozide and 92.7% smaller than with the control variant. The diameter of the zone of development of the pathogen of reticulate leaf spot Pyrenophora teres, after adding the tested preparation at a concentration of 5х10-4% to the cultivation medium, was 67.5% smaller than in the variant with tomatozide (tab. 1, fig. 2).
Tabelul 1 Table 1
Influenţa glicozidelor asupra dezvoltării miceliului fungilor The influence of glycosides on the development of fungal mycelium
Fusarium spp. şi Pyrenophora teres Fusarium spp. and Pyrenophora teres
Varianta Concentraţia, % Diametrul zonei de dezvoltare a fungilor Fusarium spp. Pyrenophora teres Drechsler cm % în comparaţie cu varianta de control cm % în comparaţie cu varianta de control Control 11,0 6,0 Suma de genistifoliozide 10-4 2,3 -79,1 2,7 -55,0 5х10-4 0,8 -92,7 1,5 -75,0 Tomatozida 10-4 9,0 -18,2 6,3 +5,0 5х10-4 6,8 -38,2 5,6 -7,5Variant Concentration, % Diameter of the growth zone of fungi Fusarium spp. Pyrenophora teres Drechsler cm % compared to the control variant cm % compared to the control variant Control 11.0 6.0 Sum of genistifoliosides 10-4 2.3 -79.1 2.7 -55.0 5х10-4 0.8 -92.7 1.5 -75.0 Tomatozide 10-4 9.0 -18.2 6.3 +5.0 5х10-4 6.8 -38.2 5.6 -7.5
- fig. 1, influenţa glicozidelor asupra dezvoltării miceliului fungilor Fusarium spp., - fig. 1, the influence of glycosides on the development of the mycelium of Fusarium spp. fungi,
- fig. 2, influenţa glicozidelor asupra dezvoltării miceliului fungilor Pyrenophora teres. - Fig. 2, the influence of glycosides on the development of the mycelium of the fungus Pyrenophora teres.
Aşadar, în testele de laborator cea mai înaltă activitate fungicidă o posedă soluţia de genistifoliozide cu concentraţia de 5х10-4%. Therefore, in laboratory tests, the highest fungicidal activity is possessed by the genistifolioside solution with a concentration of 5x10-4%.
În condiţii de câmp pe fond infectat artificial s-a studiat influenţa soluţiilor de genistifoliozide asupra răspândirii şi gradului de dezvoltare a bolilor cauzate de patogeni fungici. Plantele de grâu în faza de înfrăţire au fost tratate cu soluţii apoase ale sumei de glicozide în concentraţii de 10-4% şi 5х10-4%, calculând volumul soluţiei de lucru de 250 L/ha. Determinarea răspândirii şi a dezvoltării bolilor de grâu a fost efectuată în fazele de înfrăţire, formare a paiului, înspicare şi maturitate în lapte cu un interval de 7 zile, conform tehnicilor standard. Agenţii patogeni au fost identificaţi la microscop după prezenţa picnidiilor sau stratului infectat, forma, mărimea coloniilor şi conidiilor, precum şi sporularea patogenilor. S-a constatat că tratarea plantelor cu suma de genistifoliozide în concentraţie de 5х10-4% a redus răspândirea bolilor cu 15…17%, iar gradul de dezvoltare a bolilor cu 5…10% înainte de faza de maturitate în lapte, totodată a inhibat răspândirea bolilor cu 5…25%, iar dezvoltarea bolilor cu 5…7% înainte de coacerea deplină a boabelor. Soluţia de genistifoliozide cu concentraţia de 10-4%, aşa ca şi două concentraţii de tomatozidă, nu a avut efect semnificativ asupra rezistenţei la bolile de grâu pe parcursul întregii perioade de vegetaţie (tab. 2). In field conditions on artificially infected soil, the influence of genistifolioside solutions on the spread and degree of development of diseases caused by fungal pathogens was studied. Wheat plants in the twinning phase were treated with aqueous solutions of the sum of glycosides in concentrations of 10-4% and 5х10-4%, calculating the volume of the working solution of 250 L/ha. Determination of the spread and development of wheat diseases was carried out in the twinning, straw formation, earing and milk maturity phases with an interval of 7 days, according to standard techniques. Pathogens were identified under a microscope by the presence of pycnidia or infected layer, the shape, size of colonies and conidia, as well as sporulation of pathogens. It was found that treating plants with the sum of genistifoliosides in a concentration of 5х10-4% reduced the spread of diseases by 15…17%, and the degree of disease development by 5…10% before the milk maturity phase, at the same time inhibited the spread of diseases by 5…25%, and the development of diseases by 5…7% before full grain ripening. The solution of genistifoliosides with a concentration of 10-4%, as well as two concentrations of tomatoside, had no significant effect on the resistance to wheat diseases during the entire vegetation period (tab. 2).
Tabelul 2 Table 2
Influenţa glicozidelor asupra gradului de răspândire şi dezvoltare a bolilor pe plante de grâu de primăvară (%) Influence of glycosides on the degree of spread and development of diseases on spring wheat plants (%)
Varianta Concentraţia, % Formare a paiului Înspicare Înflorire Maturitate în lapte răspândire dezvoltare răspândire dezvoltare răspândire dezvoltare răspândire dezvoltare Control 70 24 75 80 30 30 100 57 Suma de genistifoliozide 10-4% 70 26 70 77 32 30 95 57 5х10-4% 53 19 60 63 22 20 100 45 Tomatozida 10-4% 70 28 72 80 38 35 98 59 5х10-4% 62 24 70 80 30 28 100 55Variant Concentration, % Straw formation Heading Flowering Milk maturity spread development spread development spread development spread development Control 70 24 75 80 30 30 100 57 Sum of genistifoliosides 10-4% 70 26 70 77 32 30 95 57 5х10-4% 53 19 60 63 22 20 100 45 Tomatozide 10-4% 70 28 72 80 38 35 98 59 5х10-4% 62 24 70 80 30 28 100 55
1. Кинтя П.К., Мащенко Н.Е., Марченко А.А., Швец С.А., Шуканов В.П. Регуляторы роста из отходов консервной промышленности как индукторы устойчивости злаковых культур. Матерал IX международного симпозиума Новые и нетрадиционные растения и перспективы их использования. Пущино, Москва, 2011, р. 168-171 1. Кинтя П.К., Машченко Н.Е., Марченко А.А., Shvets С.А., Shukanov В.П. Regulators of growth from the waste of the canning industry as inducers of the resistance of cereal crops. Material of the IX international symposium New and non-traditional plants and perspectives of their use. Pushchino, Moscow, 2011, р. 168-171
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| MD1192Z (en) * | 2017-02-06 | 2018-04-30 | Институт Генетики, Физиологии И Защиты Растений Академии Наук Молдовы | Glycoside preparation from Veronica officinalis L. plants with fungicidal action on Alternaria alternata |
| MD1237Z (en) * | 2017-08-10 | 2018-10-31 | Институт Микробиологии И Биотехнологии Академии Наук Молдовы | Process for presowing treatment of cereal seeds |
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| MD4301B1 (en) * | 2013-06-12 | 2014-08-31 | Institutul De Genetică, Fiziologie Şi Protecţie A Plantelor Al Academiei De Ştiinţe A Moldovei | 1-epi-5-O-alozilantirinozide for the treatment of lettuce seeds |
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Patent Citations (6)
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| MD1651F2 (en) * | 1994-01-31 | 2001-04-30 | Kemira Agro Oy | Fungi strains Nectria pityrodes Montagne possessing fungicidal properties and process for screening thereof, biofungicide, biofungicidal composition and method of fungicidal inhibition |
| MD1204G2 (en) * | 1998-09-01 | 1999-12-31 | Институт Генетики И Физиологии Растений Академии Наук Молдовы | Bioside (25R)-spirost-5-en-3b, 27-diol-27-O-(3-hydroxy-3-methylglutaroil), possesing fungicidal properties |
| MD2716G2 (en) * | 2004-04-13 | 2005-10-31 | Институт Генетики И Физиологии Растений Академии Наук Молдовы | Antistressor for winter common wheat |
| RU2355170C1 (en) * | 2008-01-15 | 2009-05-20 | Новосибирский институт органической химии им. Н.Н. Ворожцова Сибирского отделения Российской академии наук (НИОХ СО РАН) (статус государственного учреждения) | Medium for growth stimulation of green and grain crops with fungicide and antistress properties |
| EA018181B1 (en) * | 2008-02-28 | 2013-06-28 | Басф Се | Method for protecting cereals from being infected by fungi |
| MD4301B1 (en) * | 2013-06-12 | 2014-08-31 | Institutul De Genetică, Fiziologie Şi Protecţie A Plantelor Al Academiei De Ştiinţe A Moldovei | 1-epi-5-O-alozilantirinozide for the treatment of lettuce seeds |
Non-Patent Citations (2)
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| Hiroji SATO, Sadao SAKAMURA. Bitter of Tomato Seeds Isolation and Structure of a New Furostanol Saponin. Agr. Biol. Chem., 37 (2), 1973, p. 225-231 * |
| Кинтя П.К., Мащенко Н.Е., Марченко А.А., Швец С.А., Шуканов В.П. Регуляторы роста из отходов консервной промышленности как индукторы устойчивости злаковых культур. Матерал IX международного симпозиума Новые и нетрадиционные растения и перспективы их использования. Пущино, Москва, 2011, р. 168-171 * |
Also Published As
| Publication number | Publication date |
|---|---|
| MD902Y (en) | 2015-05-31 |
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| KA4Y | Short-term patent lapsed due to non-payment of fees (with right of restoration) |