CN113207914B - Application of Chinese scholartree in inhibiting fungi, bacteriostatic agent and preparation method - Google Patents

Application of Chinese scholartree in inhibiting fungi, bacteriostatic agent and preparation method Download PDF

Info

Publication number
CN113207914B
CN113207914B CN202110440041.2A CN202110440041A CN113207914B CN 113207914 B CN113207914 B CN 113207914B CN 202110440041 A CN202110440041 A CN 202110440041A CN 113207914 B CN113207914 B CN 113207914B
Authority
CN
China
Prior art keywords
extract
sophora japonica
fungi
branches
inhibiting
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN202110440041.2A
Other languages
Chinese (zh)
Other versions
CN113207914A (en
Inventor
刘悦秋
成军
张煜
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Beijing University of Agriculture
Original Assignee
Beijing University of Agriculture
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Beijing University of Agriculture filed Critical Beijing University of Agriculture
Priority to CN202110440041.2A priority Critical patent/CN113207914B/en
Publication of CN113207914A publication Critical patent/CN113207914A/en
Application granted granted Critical
Publication of CN113207914B publication Critical patent/CN113207914B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N27/00—Biocides, pest repellants or attractants, or plant growth regulators containing hydrocarbons
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
    • A01N31/02—Acyclic compounds
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
    • A01N31/08—Oxygen or sulfur directly attached to an aromatic ring system
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N35/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical
    • A01N35/02—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical containing aliphatically bound aldehyde or keto groups, or thio analogues thereof; Derivatives thereof, e.g. acetals
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/02—Saturated carboxylic acids or thio analogues thereof; Derivatives thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/36—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
    • A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
    • A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
    • A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N55/00—Biocides, pest repellants or attractants, or plant growth regulators, containing organic compounds containing elements other than carbon, hydrogen, halogen, oxygen, nitrogen and sulfur
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/08—Magnoliopsida [dicotyledons]

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Plant Pathology (AREA)
  • Dentistry (AREA)
  • Agronomy & Crop Science (AREA)
  • Zoology (AREA)
  • Environmental Sciences (AREA)
  • Pest Control & Pesticides (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention relates to application of Chinese scholartree in inhibiting fungi, a bacteriostatic agent and a preparation method thereof, belonging to the technical field of plant extraction and new application. The invention protects the application of the Chinese scholartree in inhibiting fungi on one hand, protects the application of the Chinese scholartree in preparing bacteriostatic agent on the other hand, and simultaneously protects the bacteriostatic agent based on the Chinese scholartree extract or the extractive and the preparation method thereof. The invention proves that the Chinese scholartree and the extractive/extractive thereof have obvious inhibitory activity to various plant pathogenic fungi including plant pathogenic fungi Ectoptoma polyrrostrata, plant pathogenic fungi Fusarium foetens, Colletotrichum gloeosporioides and Botrytis cinerea.

Description

Application of Chinese scholartree in inhibiting fungi, bacteriostatic agent and preparation method
Technical Field
The invention belongs to the technical field of plant extraction and new application, and particularly relates to application of Chinese scholartree in inhibiting fungi, a bacteriostatic agent and a preparation method.
Background
Sophora japonica is a tall tree, and has feathery compound leaves similar to those of Robinia pseudoacacia. The flower is light yellow, and can be cooked or used as Chinese medicine or dye. The un-bloomed sophora flower is commonly called 'sophora flower bud', which is a traditional Chinese medicine. The flowering phase is different from that of other tree species at the end of summer, and is an important honey source plant. The flowers and the pods are used as medicines, and have the effects of cooling and astringing, stopping bleeding and reducing blood pressure; the leaves and root bark have the effects of clearing away heat and toxic materials, and can be used for treating sore; the wood is used for construction. The kernel contains starch, and can be used for brewing wine or making paste and feed. Bark, branches and leaves, flower buds, flowers and seeds can be used as the medicine.
Fungi (scientific name: Fungi) is a eukaryotic, sporulating, chloroplast-free eukaryote. Including mold, yeast, mushroom, and other mushrooms known to humans. More than twelve million fungi have been discovered. Fungi are independent of animals, plants and other eukaryotes and self-form a kingdom. The fungal cells contain chitin and can produce spores through asexual propagation and sexual propagation.
Fungi are ubiquitous and may be parasitic in any organism or environment. For example, fungi may be living or parasitic in water, soil, animals, and plants. Some of the fungi that parasitize plants are beneficial to the plant, for example, the fungi of the mushrooms and the endophytic fungi of some plants enhance plant resistance, while some fungi cause plant diseases, for example, phytopathogenic fungi.
Common phytopathogenic fungi are: the plant pathogenic fungus YZ-1 (Ectophormapolyrata) causes plant root rot and has also been isolated and identified in soil. The plant pathogenic fungus YZ-2(Fusarium foetens) can cause blight of garden plants such as begonia, mallow and the like. Colletotrichum gloeosporioides is a main pathogenic bacterium causing anthracnose of plants, and can infect numerous plants such as peaches, chestnuts, mangbera, loquats, papayas, ophiopogon roots, rubber and the like. Botrytis cinerea (Botrytis cinerea) causes gray mold in a variety of vegetables and fruits such as grapes, tomatoes, strawberries, peaches, and the like.
The application of the sophora japonica in inhibiting fungi is not reported in the field.
Disclosure of Invention
Based on the blank in the field, the invention discovers the novel application of the sophora japonica in the aspect of inhibiting the fungi for the first time, and verifies that the sophora japonica extract obtained by extracting or extracting different reagents has inhibitory activity to the fungi to different degrees.
The technical scheme of the invention is as follows:
the use of Sophora japonica for inhibiting fungi is provided.
The fungus refers to a plant pathogenic fungus;
preferably, the phytopathogenic fungus is selected from the group consisting of the phytopathogenic fungi Ectophormapolytrata, the phytopathogenic fungi Fusarium foetens, Colletotrichumgloeosporioides, Botrytis cinerea.
The Sophora japonica refers to a Sophora japonica extract or a Sophora japonica extract;
preferably, the robinia pseudoacacia extract or robinia pseudoacacia extract refers to an extract or an extract of branches of robinia pseudoacacia;
preferably, the extract of the branches of acacia tree is selected from: ethanol extract and water extract of branches of Chinese scholar tree;
preferably, the extract of the branches of acacia tree is selected from: petroleum ether extract, ethyl acetate extract, n-butanol extract and water extract of branches of Chinese scholar tree.
Preferably, the bacteriostatic active ingredients of the ethanol extract of the branches of the Chinese scholar tree comprise: 2, 4-di-tert-butylphenol, 3-O-methyl-D-glucose, methyl palmitate and methyl stearate.
Preferably, the bacteriostatic active ingredients of the petroleum ether extract of the branches of the Chinese scholar tree comprise: heptadecane, 3, 5-bis (1, 1-dimethylethyl) phenol, n-hexadecanoic acid, ethyl 2-hydroxy-1- (hydroxymethyl) hexadecanoate;
in other embodiments of this group, the bacteriostatic active ingredients of the ethyl acetate extract of the branches of acacia tree include: 3-acetyl-2-octanone, n-hexadecanoic acid and octa-alkyl tert-butyl pentafluoropropionate.
In some embodiments of this group, the bacteriostatic active ingredient of the n-butanol extract of the branches of acacia tree comprises: 2, 4-di-tert-butylphenol, 3-O-methyl-D-fructose, 2-chloroethanol, triisobutylsilyl ether and methyl stearate.
In other embodiments of this group, the bacteriostatic active ingredient of the aqueous extract of the branches of acacia tree comprises: 2-chloroethanol, triisobutylsilyl ether, methyl stearate and methyl palmitate.
The use of Sophora japonica in inhibiting fungi comprises treating fungi with Sophora japonica extract or aqueous solution of Sophora japonica extract; preferably, the concentration of the Sophora japonica extract or aqueous solution of the Sophora japonica extract is 0.01mg/ml to 100 mg/ml.
The application of Sophora japonica in preparing bacteriostatic agent is provided.
The bacteriostasis means inhibiting or killing fungi;
preferably, the fungus refers to a plant pathogenic fungus;
preferably, the phytopathogenic fungus is selected from the group consisting of the phytopathogenic fungi Ectophormapolytrata, the phytopathogenic fungi Fusarium foetens, Colletotrichumgloeosporioides, Botrytis cinerea;
preferably, the sophora japonica refers to sophora japonica extract or sophora japonica extract;
preferably, the robinia pseudoacacia extract or robinia pseudoacacia extract refers to an extract or an extract of branches of robinia pseudoacacia;
preferably, the extract of the branches of acacia tree is selected from: ethanol extract and water extract of branches of Chinese scholar tree;
preferably, the extract of the branches of acacia tree is selected from: petroleum ether extract, ethyl acetate extract, n-butanol extract and water extract of branches of Chinese scholar tree.
A bacteriostatic agent is characterized in that the active component of the bacteriostatic agent comprises a Chinese scholar tree extract or a Chinese scholar tree extract.
The bacteriostasis means inhibiting or killing fungi;
preferably, the fungus refers to a plant pathogenic fungus;
preferably, the phytopathogenic fungus is selected from the group consisting of the phytopathogenic fungi Ectophormapolytrata, the phytopathogenic fungi Fusarium foetens, Colletotrichumgloeosporioides, Botrytis cinerea;
preferably, the robinia pseudoacacia extract or robinia pseudoacacia extract refers to an extract or an extract of branches of robinia pseudoacacia;
preferably, the extract of the branches of acacia tree is selected from: ethanol extract and water extract of branches of Chinese scholar tree;
preferably, the extract of the branches of acacia tree is selected from: petroleum ether extract, ethyl acetate extract, n-butanol extract, and water extract of branches of Sophora japonica L;
preferably, the active ingredients of the bacteriostatic agent include: an aqueous solution of a Sophora japonica extract or an extract of Sophora japonica;
preferably, the concentration of the sophora japonica extract or the aqueous solution of the sophora japonica extract is 0.01mg/ml to 100 mg/ml;
preferably, the bacteriostatic agent further comprises a pharmaceutical adjuvant;
preferably, the pharmaceutical excipient is selected from: solvents, propellants, solubilizers, cosolvents, emulsifiers, colorants, binders, disintegrants, fillers, lubricants, wetting agents, osmotic pressure regulators, stabilizers, glidants, flavoring agents, preservatives, suspending agents, coating materials, fragrances, anti-adhesives, integration agents, permeation promoters, pH regulators, buffers, plasticizers, surfactants, foaming agents, antifoaming agents, thickeners, encapsulation agents, humectants, absorbents, diluents, flocculants and deflocculants, filter aids, release retardants.
A preparation method of a bacteriostatic agent is characterized in that a Chinese scholar tree extract or a Chinese scholar tree extract is used as an active ingredient of the bacteriostatic agent.
The extraction comprises the following steps: adding a sophora japonica sample into a solvent, boiling, carrying out suction filtration, and concentrating the obtained filtrate to obtain an extract;
preferably, the solvent is 85% -95%, preferably 95% ethanol;
preferably, the feed-liquid ratio of the sophora japonica sample to the solvent is 1-5 g: 5-75ml, preferably 1g:10 ml;
preferably, the cooking is at 50-80 ℃, preferably 60 ℃ for 4-8 hours, preferably 6 hours;
preferably, the extraction refers to extracting and concentrating the extract obtained by extraction by using an extracting agent;
preferably, the extractant is selected from: petroleum ether, ethyl acetate, n-butanol and water;
preferably, the extraction condition is that the volume ratio of the extracting agent to the water is 1: 0.5-1.5, preferably 1:1 at 20-30 ℃, preferably 25 ℃, and the mixture is kept stand for 25min-35min, preferably 30 min;
preferably, the concentration refers to evaporating the solvent or extractant to dryness with a rotary evaporator;
preferably, the acacia sample refers to powder obtained by drying branches of acacia in the shade and then crushing.
The invention obtains the association between the Chinese scholartree and a plurality of fungi through a large number of bacteriostasis tests and screens, and pioneers the new application of the Chinese scholartree in inhibiting the fungi. The invention protects the application of the Chinese scholartree in inhibiting fungi on one hand, protects the application of the Chinese scholartree in preparing bacteriostatic agent on the other hand, and simultaneously protects the bacteriostatic agent based on the Chinese scholartree extract or the extractive and the preparation method thereof. Experiments prove that the Chinese scholartree extract obtained by extracting various extracting agents (comprising ethyl acetate, petroleum ether, n-butyl alcohol, water and the like) or the Chinese scholartree extract obtained by extracting a solvent (ethanol or water) has obvious inhibitory activity on various plant pathogenic fungi including plant pathogenic fungi such as Ectophomamamultirestrata, plant pathogenic fungi Fusarium foetens, Colletotrichumgloeosporioides and Botrytis cinerea. Meanwhile, component detection shows that the sophora japonica extract or the bacteriostatic active substance of the sophora japonica extract with different extracting agents or solvents comprises the following components: 2, 4-di-tert-butylphenol, 3-O-methyl-D-glucose, heptadecane, 3, 5-bis (1, 1-dimethylethyl) phenol, n-hexadecanoic acid, ethyl 2-hydroxy-1- (hydroxymethyl) hexadecanoate, 3-acetyl-2-octanone, octa-tert-butyl pentafluoropropionate, triisobutylsilyl ether, methyl stearate, 2-chloroethanol, triisobutylsilyl ether, methyl stearate, methyl hexadecanoate.
Drawings
FIG. 1 is a mass spectrum total ion flow diagram of an ethanol extract of branches of Sophora japonica provided in the experimental section of the present invention.
Fig. 2 is a mass spectrum total ion flow diagram of the petroleum ether extract from branches of robinia pseudoacacia provided in the experimental part of the present invention.
Fig. 3 is a mass spectrum total ion flow diagram of the ethyl acetate extract of robinia pseudoacacia branches provided in the experimental part of the present invention.
FIG. 4 is a mass spectrum total ion flow diagram of n-butanol extract of branches of Sophora japonica according to the experimental example of the present invention.
Fig. 5 is a mass spectrum total ion flow diagram of the robinia branch water extract provided in the experimental part of the present invention.
Detailed Description
The present invention will be described in further detail with reference to specific examples, but the scope of the present invention is not limited thereto.
Sources of biological material
Plant experimental materials used in the experimental part of the present invention: the annual and biennial branches of sophora japonica are collected in the campus of the applicant unit, and the branches can also be obtained by commercially obtaining sophora japonica plants.
The strain is as follows: plant pathogenic fungi YZ-1 (Ectoptomultidata), plant pathogenic fungi YZ-2(Fusarium foetens), colletotrichum gloeosporioides (Colletotrichum gloeosporioides), and Botrytis cinerea (Botrytis cinerea) are all commercially available.
The reagent consumables adopted in the experimental example part of the invention are as follows: ethanol, n-butanol, ethyl acetate, petroleum ether, water, etc. are commercially available
Group 1 example, novel bacteriostatic application of Sophora japonica
The present group of examples provides the use of acacia in inhibiting fungi.
In light of the present disclosure, one skilled in the art can derive the teaching to use any part, organ, tissue of Sophora japonica, for example: roots, trunks, resins, leaves, flowers, fruits, etc. are used to inhibit fungi, or in any part, organ, tissue of acacia, for example: the product prepared from root, trunk, resin, leaf, flower, fruit, etc. is used for inhibiting fungi. Any part, organ, tissue using acacia, for example: roots, trunks, resins, leaves, flowers, fruits, etc. are used to inhibit fungi, or in any part, organ, tissue of acacia, for example: the action of the products prepared by taking roots, trunks, resins, leaves, flowers, fruits and the like as raw materials for inhibiting fungi falls into the protection scope of the invention.
In specific embodiments, the fungus refers to a plant pathogenic fungus;
preferably, the phytopathogenic fungus is selected from the group consisting of the phytopathogenic fungi Ectophormapolytrata, the phytopathogenic fungi Fusarium foetens, Colletotrichumgloeosporioides, Botrytis cinerea.
The fungal targets to be inhibited by the sophora japonica or the products derived from sophora japonica according to the teachings of the present invention are not limited to the above-mentioned plant pathogenic fungi, but the inhibition of other common plant pathogenic fungi in the art can be attempted by the sophora japonica or the products derived from sophora japonica, for example, the inhibition of cotton mold (Achlya), Pythium mold (Pythium), Phytophthora (Phytophthora), white rust (Albugo), downy mildew (Peronospora), powdery mildew (Erysiphe), cystobacterium (gibberlla), Venturia (Venturia), Sclerotinia (scleraria), rust in basidiomycetes, Ustilago (Ustilago), deuteromycetes, and the like can be achieved, and the inhibitory activity and effect similar to those of the present invention can be expected. Any use of Sophora japonica or products derived therefrom for inhibiting the action of the plant pathogenic fungi Ectophomama polyrata, the plant pathogenic fungi Fusarium foetens, Colletotrichum gloeosporioides, Botrytis cinerea, Aphlya gossypii (Achlya), Pythium species (Pyrium), Phytophthora species (Phytophthora), white rust species (Albugo), downy mildew species (Peronospora), powdery mildew species (Erysiphe), Saccharomycetes (Gierbella), Venturia nigra (Venturia), Sclerotinia species (Sclerotinia), Russia sp, Ustilago (Ustilago) falls within the scope of the present invention.
In more specific embodiments, the sophora japonica refers to sophora japonica extract or sophora japonica extract;
preferably, the robinia pseudoacacia extract or robinia pseudoacacia extract refers to an extract or an extract of branches of robinia pseudoacacia;
preferably, the extract of the branches of acacia tree is selected from: ethanol extract and water extract of branches of Chinese scholar tree;
preferably, the extract of the branches of acacia tree is selected from: petroleum ether extract, ethyl acetate extract, n-butanol extract and water extract of branches of Chinese scholar tree.
In some embodiments, the use of acacia in inhibiting fungi refers to treating the fungi with an acacia extract or an aqueous solution of the acacia extract; preferably, the concentration of the Sophora japonica extract or aqueous solution of the Sophora japonica extract is 0.01mg/ml to 100 mg/ml.
Group 2 example, use of Sophora japonica of the present invention in the preparation of bacteriostatic agents
The group of embodiments provides the use of sophora japonica in preparing bacteriostatic agents.
In light of the present disclosure, one skilled in the art can derive the teaching to use any part, organ, tissue of Sophora japonica, for example: roots, trunks, resins, leaves, flowers, fruits, etc. are used for preparing bacteriostatic agents, or in any part, organ, tissue of sophora japonica, for example: the product prepared by using root, trunk, resin, leaf, flower, fruit and the like as raw materials is used for preparing the bacteriostatic agent. Any part, organ, tissue using acacia, for example: roots, trunks, resins, leaves, flowers, fruits, etc. are used for preparing bacteriostatic agents, or in any part, organ, tissue of sophora japonica, for example: the behavior of preparing bacteriostatic agent from root, trunk, resin, leaf, flower, fruit and the like, or writing bacteriostatic application on package boxes of related products of Chinese scholartree, or adding related components of Chinese scholartree into bacteriostatic products falls into the protection scope of the invention.
In some embodiments, the bacteriostatic refers to inhibiting or killing fungi;
preferably, the fungus refers to a plant pathogenic fungus;
preferably, the phytopathogenic fungus is selected from the group consisting of the phytopathogenic fungi Ectophormapolytrata, the phytopathogenic fungi Fusarium foetens, Colletotrichumgloeosporioides, Botrytis cinerea;
preferably, the sophora japonica refers to sophora japonica extract or sophora japonica extract;
preferably, the robinia pseudoacacia extract or robinia pseudoacacia extract refers to an extract or an extract of branches of robinia pseudoacacia;
preferably, the extract of the branches of acacia tree is selected from: ethanol extract and water extract of branches of Chinese scholar tree;
preferably, the extract of the branches of acacia tree is selected from: petroleum ether extract, ethyl acetate extract, n-butanol extract and water extract of branches of Chinese scholar tree.
Group 3 examples of bacteriostatic agents according to the invention
The present group of embodiments provides a bacteriostatic agent. All embodiments of this group share the following common features: the active component of the bacteriostatic agent comprises a Chinese scholar tree extract or a Chinese scholar tree extract.
Any part, organ, tissue of acacia can be used by those skilled in the art in light of the teachings of the present invention, for example: extracting or extracting roots, trunks, resins, leaves, flowers, fruits and the like and using the extract or the extract as an active ingredient of the bacteriostatic agent, or extracting any part, organ, tissue of the sophora japonica, for example: the bacteriostatic agent is prepared by further processing extracts or extracts obtained by extracting or extracting roots, trunks, resins, leaves, flowers, fruits and the like serving as raw materials. Any part, organ, combination of acacia, for example: extracts or extractives of roots, trunks, resins, leaves, flowers, fruits and the like are taken as active ingredients or main materials, auxiliary materials, monarch drugs and ministerial drugs are added, prepared and produced to be bacteriostatic, or any part, organ and tissue containing the Chinese scholartree, for example: the action of the antibacterial application is written on the related product packaging box of the extracts or the extracts of roots, trunks, resins, leaves, flowers, fruits and the like, or any part, organ and tissue of Chinese scholartree is added in the antibacterial product, for example: extracts or behaviors of extracts of roots, trunks, resins, leaves, flowers, fruits, etc. fall within the scope of the present invention.
In some embodiments, the bacteriostatic refers to inhibiting or killing fungi;
preferably, the fungus refers to a plant pathogenic fungus;
preferably, the phytopathogenic fungus is selected from the group consisting of the phytopathogenic fungi Ectophormapolytrata, the phytopathogenic fungi Fusarium foetens, Colletotrichumgloeosporioides, Botrytis cinerea;
preferably, the robinia pseudoacacia extract or robinia pseudoacacia extract refers to an extract or an extract of branches of robinia pseudoacacia;
preferably, the extract of the branches of acacia tree is selected from: ethanol extract and water extract of branches of Chinese scholar tree;
preferably, the extract of the branches of acacia tree is selected from: petroleum ether extract, ethyl acetate extract, n-butanol extract, and water extract of branches of Sophora japonica L;
in some embodiments of this group, the bacteriostatic active ingredient of the ethanol extract of the branches of acacia tree comprises: 2, 4-di-tert-butylphenol, 3-O-methyl-D-glucose, methyl palmitate and methyl stearate.
In some embodiments of this group, the bacteriostatic active ingredient of the petroleum ether extract of acacia branches comprises: heptadecane, 3, 5-bis (1, 1-dimethylethyl) phenol, n-hexadecanoic acid, 2-hydroxy-1- (hydroxymethyl) hexadecanoic acid ethyl ester.
In other embodiments of this group, the bacteriostatic active ingredients of the ethyl acetate extract of the branches of acacia tree include: 3-acetyl-2-octanone, n-hexadecanoic acid and octa-alkyl tert-butyl pentafluoropropionate.
In some embodiments of this group, the bacteriostatic active ingredient of the n-butanol extract of the branches of acacia tree comprises: 2, 4-di-tert-butylphenol, 3-O-methyl-D-fructose, 2-chloroethanol, triisobutylsilyl ether and methyl stearate.
In other embodiments of this group, the bacteriostatic active ingredient of the aqueous extract of the branches of acacia tree comprises: 2-chloroethanol, triisobutylsilyl ether, methyl stearate and methyl palmitate.
Preferably, the active ingredients of the bacteriostatic agent include: an aqueous solution of a Sophora japonica extract or an extract of Sophora japonica;
preferably, the concentration of the sophora japonica extract or the aqueous solution of the sophora japonica extract is 0.01mg/ml to 100 mg/ml;
preferably, the bacteriostatic agent further comprises a pharmaceutical adjuvant;
preferably, the pharmaceutical excipient is selected from: solvents, propellants, solubilizers, cosolvents, emulsifiers, colorants, binders, disintegrants, fillers, lubricants, wetting agents, osmotic pressure regulators, stabilizers, glidants, flavoring agents, preservatives, suspending agents, coating materials, fragrances, anti-adhesives, integration agents, permeation promoters, pH regulators, buffers, plasticizers, surfactants, foaming agents, antifoaming agents, thickeners, encapsulation agents, humectants, absorbents, diluents, flocculants and deflocculants, filter aids, release retardants.
EXAMPLE 4 preparation of bacteriostatic Agents of the invention
The present group of embodiments provides a method for preparing a bacteriostatic agent. All embodiments of this group share the following common features: uses the Chinese scholartree extract or the Chinese scholartree extract as an active component of the bacteriostatic agent.
Any part, organ, tissue of sophora japonica, for example: extracts or extractives of roots, trunks, resins, leaves, flowers, fruits and the like are taken as active ingredients or main materials, auxiliary materials, monarch drugs and ministerial drugs are added, prepared and produced to be bacteriostatic, or any part, organ and combination containing the Chinese scholar tree, for example: the behavior of the antibacterial application is written on the related product packaging box of the extracts or extracts of roots, trunks, resins, leaves, flowers, fruits and the like, or any part, organ and combination of Chinese scholartree are added in the antibacterial product, for example: extracts or behaviors of extracts of roots, trunks, resins, leaves, flowers, fruits, etc. fall within the scope of the present invention.
In some embodiments, the extracting comprises: adding a sophora japonica sample into a solvent, boiling, carrying out suction filtration, and concentrating the obtained filtrate to obtain an extract;
preferably, the solvent is 85% -95%, preferably 95% ethanol;
preferably, the feed-liquid ratio of the sophora japonica sample to the solvent is 1-5 g: 5-75ml, preferably 1g:10 ml;
preferably, the cooking is at 50-80 ℃, preferably 60 ℃ for 4-8 hours, preferably 6 hours;
preferably, the extraction refers to extracting and concentrating the extract obtained by extraction by using an extracting agent;
preferably, the extractant is selected from: petroleum ether, ethyl acetate, n-butanol and water;
preferably, the extraction condition is that the volume ratio of the extracting agent to the water is 1: 0.5-1.5, preferably 1:1 at 20-30 ℃, preferably 25 ℃, and the mixture is kept stand for 25min-35min, preferably 30 min;
preferably, the concentration refers to evaporating the solvent or extractant to dryness with a rotary evaporator;
preferably, the acacia sample refers to powder obtained by drying branches of acacia in the shade and then crushing.
Experimental example, concrete experimental operation and effect verification of novel antibacterial application of Chinese scholartree
A,Experimental methods
PDA culture Medium configuration
Cutting 200g peeled potato into small pieces, placing into a pot, adding 1000ml pure water, boiling for 30-40 min, adding 20g weighed glucose and 15g agar, stirring, packaging, and sterilizing at 121 deg.C for 30 min.
2. Sample preparation
(1) Milling powder
Cleaning collected branches of Chinese scholartree with pure water to remove dust on the surface, cutting into small segments, drying in the shade indoors, weighing 10Kg branches of Chinese scholartree after drying in the shade, and grinding into powder by a grinder for later use.
(2) Hot reflux extraction
Mixing 10Kg of powder of branches of Chinese scholartree with 95% ethanol solution at a ratio of 1:10, boiling at 60 deg.C for 6 hr by using a thermal reflux device, filtering the residue with a suction filtration device, and steaming the filtrate to obtain 1160g of extract with a yield of 11.6%.
(3) Systematic solvent extraction
Extracting the obtained robinia pseudoacacia branch extract by using a system solvent extraction method, suspending the extract in water, dividing the suspension into a plurality of parts, extracting by using petroleum ether, ethyl acetate, n-butyl alcohol and water respectively to obtain a petroleum ether extract, an ethyl acetate extract, an n-butyl alcohol extract and a water extract, performing rotary evaporation and cold drying to obtain extract powder of each component, wherein the weight of the extract powder is 314.36g, 368.30g, 131.89g and 345.33 g. The extract (extract) and the extracts (powder) of the components are respectively prepared into 100mg/ml sample aqueous solution containing 1% of Tween-80 and 2% of dimethyl sulfoxide, and then the sample aqueous solution is diluted into 10mg/ml, 1mg/ml, 0.1mg/ml and 0.01mg/ml sample solutions by pure water gradient for later use.
3. Mass spectrometric total composition analysis
Adding the extract of the branches of the Chinese scholartree, petroleum ether extract, ethyl acetate extract, n-butanol extract and water extract into chromatographically pure methanol, preparing into 50mg/L solution, and analyzing the components by GC-MS analysis under the analysis and detection conditions shown in the following table 1.
TABLE 1. Sophora japonica branch extract and extraction site mass spectrometry conditions
Figure RE-GDA0003103181380000091
4. Determination of bacteriostatic Activity
Sucking 1ml of the prepared sample solution culture medium in a clean bench, pouring into a culture dish with the diameter of 90mm of a 9ml PDA culture medium, fully mixing and shaking up to prepare a uniform culture medium with the medicine; then inoculating the bacteria cake in the activated test bacterial colony in a culture dish by adopting a growth rate method, cutting the bacteria cake by using a 5mm sterile puncher, and inoculating the bacteria cake to a culture medium with medicines, wherein 1 bacteria cake is inoculated in each dish; finally, placing the culture dish with the inoculated bacteria in a constant temperature incubator at 28 ℃ for culturing for 72h, measuring the diameter of a bacterial colony by a cross method, calculating the inhibition rate, and then solving a toxicity regression equation and an effective medium concentration EC50And the like, and the SPSS26.0 is combined for differential analysis.
II,Experiment ofResults
1. Results of component detection
The gc-ms analysis result chart of the ethanol extract of branches of Chinese scholar tree and the extracted components is shown in figure 1. The chemical compositions of the components are shown in the following table 2.
TABLE 2. identification of chemical components of ethanol extract from branches of Sophora japonica
Figure RE-GDA0003103181380000101
Figure RE-GDA0003103181380000111
The gc-ms analysis result chart of each component of the petroleum ether extract of the branches of Chinese scholar trees is shown in figure 2. The chemical compositions of the components are shown in the following table 3.
TABLE 3. evaluation result of chemical components of petroleum ether extract from branches of Chinese scholar tree
Figure RE-GDA0003103181380000112
Figure RE-GDA0003103181380000121
The gc-ms analysis result chart of each component of the ethyl acetate extract of the branches of Chinese scholar trees is shown in figure 3. The chemical compositions of the components are shown in the following table 4.
TABLE 4. identification of chemical components of ethyl acetate extract from branches of Sophora japonica
Figure RE-GDA0003103181380000122
Figure RE-GDA0003103181380000131
The gc-ms analysis result chart of each component of the n-butanol extract of the branches of the Chinese scholar tree is shown in figure 4. The chemical compositions of the components are shown in the following table 5.
TABLE 5. evaluation result of chemical components of n-butanol extract from branches of Japanese pagodatree
Figure RE-GDA0003103181380000132
FIG. 5 shows the gc-ms analysis result of each component of n-butanol extract of branches of Chinese scholar tree. The chemical compositions of the components are shown in the following table 6.
TABLE 6 chemical component identification results of the branch water extract of Sophora japonica
Figure RE-GDA0003103181380000133
Figure RE-GDA0003103181380000141
2. Result of bacteriostasis
The results of bacteriostasis (table 7) show that: the best effect on the No. 1 bacterium at 48h is the ethyl acetate extract of the branches of Chinese scholartree, and the EC of the extract505.13g/L, and then water extract, ethanol extract, n-butanol extract, petroleum ether extract; 72h of the extract with best effect on the No. 1 bacterium is the ethyl acetate extract of the branches of Chinese scholartree, and the EC of the extract5014.66g/L, followed by ethanol extract, n-butanol extract, water extract, petroleum ether extract. The best effect on the No. 2 bacterium at 48h is the ethyl acetate extract of the branches of Chinese scholartree, and the EC of the extract5016.47g/L, and then n-butanol extract, ethanol extract, water extract, petroleum ether extract; the best 72h effect on the bacteria No. 2 is the ethyl acetate extract of the branches of Chinese scholartree, and the EC of the extract5011.49g/L, followed by n-butanol extract, ethanol extract, petroleum ether extract, water extract. The best effect on colletotrichum gloeosporioides at 48h is the ethyl acetate extract of the branches of Chinese scholartree, the EC of which502.58g/L, and then n-butanol extract, ethanol extract, water extract, petroleum ether extract;table 8 shows that 72h of colletotrichum gloeosporioides most effective is the ethyl acetate extract of the branches of Chinese scholar tree, the EC of which502.88g/L, followed by n-butanol extract, ethanol extract, water extract, petroleum ether extract. The best effect on the botrytis cinerea at 48h is the ethyl acetate extract of the branches of Chinese scholartree, the EC of which is509.02g/L, petroleum ether extract, n-butanol extract, ethanol extract, and water extract; the best 72h effect on Botrytis cinerea is the ethyl acetate extract of the branches of Chinese scholartree, and the EC of the extract5016.68g/L, followed by petroleum ether extract, n-butanol extract, water extract, ethanol extract. In a whole, the crude extract of the branches of the Chinese scholartree has certain inhibition effects on four strains in 48 hours and 72 hours, and the inhibition effects are positively correlated with the concentration of a sample solution. Four strains for ethyl acetate extract EC of branches of Chinese scholartree50The value was the smallest and the slope was also the largest among the five crude extracts, so the sensitivity was the highest among the five crude extracts, followed by n-butanol extract, of which the least sensitive to bacteria # 1, 2 and colletotrichum gloeosporioides was petroleum ether extract, the least sensitive to botrytis cinerea was water extract at 48h, and the least sensitive to 72h ethanol extract. According to experimental results, active substances of the branches of the Chinese scholartree mainly exist in ethyl acetate and n-butyl alcohol extracts, and the two extracts can be used as main directions for separating and purifying monomer compounds, so that a foundation is laid for separating and purifying a large amount of active monomer compounds in the future.
In addition, the result of the antibacterial activity difference analysis of the branch extract and the extracted part of the Chinese scholar tree is that no obvious difference exists in the group, and a certain difference exists between the groups.
TABLE 7 toxicity regression analysis table of Robinia pseudoacacia branch crude extract 48h
Figure RE-GDA0003103181380000151
TABLE 8 toxicity regression analysis table of crude extract of branches of Sophora japonica for 72h
Figure RE-GDA0003103181380000152

Claims (14)

1. Use of Sophora japonica for inhibiting fungi, characterized in that the fungi are selected from the group consisting of phytopathogenic fungiEctophomamultirostrataPlant pathogenic fungiFusarium foetensA group of (a); the Sophora japonica refers to a Sophora japonica extract or a Sophora japonica extract; the sophora japonica extract refers to an ethanol extract of sophora japonica branches; the Sophora japonica extract refers to petroleum ether extract, ethyl acetate extract, n-butanol extract or water extract of the ethanol extract of branches of Sophora japonica.
2. Use of Sophora japonica according to claim 1 for inhibiting fungi, wherein the fungi are treated with Sophora japonica extract or an aqueous solution of Sophora japonica extract.
3. Use of Sophora japonica according to claim 2, wherein the concentration of the Sophora japonica extract or the aqueous solution of the Sophora japonica extract is 0.01mg/ml to 100 mg/ml.
4. The application of the Chinese scholartree in preparing the bacteriostatic agent is characterized in that the bacteriostatic agent inhibits or kills fungi; the fungus is selected from the group consisting of plant pathogenic fungiEctophomamultirostrataPlant pathogenic fungiFusarium foetensA group of (a); the Sophora japonica refers to a Sophora japonica extract or a Sophora japonica extract; the sophora japonica extract refers to an ethanol extract of sophora japonica branches; the Sophora japonica extract refers to petroleum ether extract, ethyl acetate extract, n-butanol extract or water extract of the ethanol extract of branches of Sophora japonica.
5. Use of Sophora japonica according to any one of claims 1 to 3 for inhibiting fungi, wherein the extraction comprises: adding a sophora japonica sample into a solvent, boiling, carrying out suction filtration, and concentrating the obtained filtrate to obtain an extract; the solvent is 85-95% ethanol;
the extraction refers to extracting and concentrating the extract obtained by extraction by using an extracting agent; the extractant is selected from: petroleum ether, ethyl acetate, n-butanol and water;
the bacteriostasis means inhibiting or killing fungi; the fungus refers to a plant pathogenic fungus; the plant pathogenic fungi is selected from plant pathogenic fungiEctophomamultirostrataPlant pathogenic fungiFusarium foetensGroup (d) of (a).
6. Use of Sophora japonica according to claim 5, wherein the solvent is 95% ethanol.
7. The use of Sophora japonica according to claim 5 for inhibiting fungi, wherein the feed-to-solution ratio of Sophora japonica sample to solvent is 1-5 g: 5-75 ml.
8. Use of Sophora japonica according to claim 7, wherein the feed-to-solution ratio of the Sophora japonica sample to the solvent is 1g:10 ml.
9. Use of Sophora japonica according to claim 5 for inhibiting fungi, wherein the boiling is at 50-80 ℃ for 4-8 hours.
10. Use of sophora japonica according to claim 9 for inhibiting fungi, wherein the boiling is at 60 ℃ for 6 hours.
11. The use of Sophora japonica according to claim 5 for inhibiting fungi, wherein the extraction conditions are 20-30 deg.C, and the volume ratio of the extractant to water is 1: 0.5-1.5, and the mixture is allowed to stand for 25-35 min.
12. The use of Sophora japonica according to claim 11 for inhibiting fungi, wherein the extraction conditions are mixing and standing at 25 ℃ for 30min at a volume ratio of extractant to water of 1: 1.
13. Use of Sophora japonica according to claim 5 for inhibiting fungi, wherein the concentration is achieved by evaporating the solvent or extractant to dryness using a rotary evaporator.
14. Use of Sophora japonica according to claim 5 for inhibiting fungi, wherein the Sophora japonica sample is powder obtained by drying branches of Sophora japonica in the shade and pulverizing.
CN202110440041.2A 2021-04-23 2021-04-23 Application of Chinese scholartree in inhibiting fungi, bacteriostatic agent and preparation method Active CN113207914B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202110440041.2A CN113207914B (en) 2021-04-23 2021-04-23 Application of Chinese scholartree in inhibiting fungi, bacteriostatic agent and preparation method

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202110440041.2A CN113207914B (en) 2021-04-23 2021-04-23 Application of Chinese scholartree in inhibiting fungi, bacteriostatic agent and preparation method

Publications (2)

Publication Number Publication Date
CN113207914A CN113207914A (en) 2021-08-06
CN113207914B true CN113207914B (en) 2022-04-12

Family

ID=77088699

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202110440041.2A Active CN113207914B (en) 2021-04-23 2021-04-23 Application of Chinese scholartree in inhibiting fungi, bacteriostatic agent and preparation method

Country Status (1)

Country Link
CN (1) CN113207914B (en)

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003000898A1 (en) * 2001-06-22 2003-01-03 Syngenta Participations Ag Plant genes involved in defense against pathogens
CN101502274A (en) * 2009-03-11 2009-08-12 南京信息工程大学 Plant source pesticide for inhibiting rice blast and application thereof in aspect for inhibiting rice blast
CN102205022A (en) * 2011-05-25 2011-10-05 吴培春 Chinese medicinal preparation for treating mycotic infection and preparation method thereof
CN107173398A (en) * 2017-06-07 2017-09-19 江苏徐淮地区淮阴农业科学研究所 Prevent and treat the botanical fungicide of wheat scab

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NL2015316B1 (en) * 2015-08-18 2017-03-07 Rede Invest B V Method for treating pests and/or infections.

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003000898A1 (en) * 2001-06-22 2003-01-03 Syngenta Participations Ag Plant genes involved in defense against pathogens
CN101502274A (en) * 2009-03-11 2009-08-12 南京信息工程大学 Plant source pesticide for inhibiting rice blast and application thereof in aspect for inhibiting rice blast
CN102205022A (en) * 2011-05-25 2011-10-05 吴培春 Chinese medicinal preparation for treating mycotic infection and preparation method thereof
CN107173398A (en) * 2017-06-07 2017-09-19 江苏徐淮地区淮阴农业科学研究所 Prevent and treat the botanical fungicide of wheat scab

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
22种植物提取物对番茄病原菌生物活性的筛选;杨苏苏 等;《山西农业科学》;20181231;第46卷(第3期);第1.1-1.2部分、表1-2 *
28种植物样品提取物离体抑菌作用测定;于平儒 等;《徐州师范大学学报(自然科学版)》;20031231(第4期);第1.1-1.3部分、表1-2 *
9种植物提取物对植物病原真菌的生物活性筛选;郝明亮 等;《现代农药》;20101231;第9卷(第4期);第1.2、2.1部分、表1-2 *
国槐根次生代谢成分提取纯化与生物活性研究;樊肃;《中国优秀硕士学位论文全文数据库 工程科技Ⅰ辑》;20180215(第2期);摘要、第13页2.2.2部分、第35页3.4.3部分 *

Also Published As

Publication number Publication date
CN113207914A (en) 2021-08-06

Similar Documents

Publication Publication Date Title
CN109843068B (en) Use of a cell extract of one or more microalgae of the genus prototheca for fungicidal and/or bactericidal activity against fungi, oomycetes and/or pathogenic bacteria of plants and cultured seeds
De Curtis et al. Integration of biological and chemical control of brown rot of stone fruits to reduce disease incidence on fruits and minimize fungicide residues in juice
Adekunle et al. Nematicidal effects of Leucaena leucocephala and Gliricidia sepium extracts on Meloidogyne incognita infecting okra
John et al. Role of Allelopathy in vegetables crops production.
KR20110089157A (en) Plant extracts for use as phytochemicals
KR101293107B1 (en) A composite for a prevention and a removal of disease of plants and a soil conditioning, and manufacturing method of the same
KR100754053B1 (en) Compositions inducing plant disease-resistance and process for producing the same
Abbas et al. Phytotoxicity of Fusarium, other fungal isolates, and of the phytotoxins fumonisin, fusaric acid, and moniliformin to jimsonweed
KR20180008960A (en) Composition for controlling plant diseases comprising an extract of Maesa japonica, and method for controlling plant diseases using the same
Dong et al. Stimulatory effects of wheat (Triticum aestivum L.) on seed germination of Orobanche minor Sm
KR102159034B1 (en) Composition for controlling cobweb disease comprising extract of Hosta longissima HONDA as an active ingradient and uses thereof
CN113207914B (en) Application of Chinese scholartree in inhibiting fungi, bacteriostatic agent and preparation method
KR20110096109A (en) Compositions for protecting plant diseases
CN109122727A (en) Application of colletotrichum gloeosporioides BWH-1 in preparation of herbicide
CN112136824B (en) Application of quassin compounds in preventing and treating plant fungal diseases
CN105838613A (en) Pigeon pea endophytic fungi high in yield of flavipin and application of pigeon pea endophytic fungi
Afzal et al. Management of Fusarium Basal Rot of Onion caused by Fusarium oxysporum f. sp. cepae through Desert Plants Extracts.
KR100832745B1 (en) Composition for controlling plant diseases which comprises lignan compounds, resorcinol compounds or nutmeg extract and method for controlling plant disease using same
Oh et al. Effect of seed disinfection on Bakanae disease in Ginkgo biloba outer seed coat extract.
KR101929357B1 (en) Composition for controlling cobweb disease comprising extract of Liriope platyphylla as an active ingradient and uses thereof
Hashem et al. Bio-herbicidal potential of desert plants Artemisia judaica L., Asphodelus microcarpus Salzm. &Viv. and Solanum nigrum L. against Portulaca oleracea and Phalaris minor
Abu Irmaileh et al. Antifungal activity of the stinkwort (Inula graveolens) extracts
KR102336864B1 (en) Composition for controling plant diseases comprising Hosta longissima extracts and fractions, and uses thereof
KR100865880B1 (en) Natural plant extracts and composition for controlling pest comprising the same
CN108902149A (en) Scopoletin excites the purposes and application method of the broad spectrum resistance of plant immune

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant