KR101293107B1 - A composite for a prevention and a removal of disease of plants and a soil conditioning, and manufacturing method of the same - Google Patents

Abstract

PURPOSE: A composition for preventing and removing pathogens in a plant and for soil remediation is provided to effectively prevent and remove pathogens and to improve and condition soil without a disturbance in ecosystems. CONSTITUTION: A method for manufacturing a composition for preventing and removing pathogens in a plant and for soil remediation comprises the steps of: adding 300-1000 parts by weight of water to 100 parts by weight of Ailanthus altissima or Acanthopanax and heating at 50-120 °C for 5-10 hours to obtain a hot water extract; mixing 300-800 parts by weight of rice bran dried at -20 °C to 120 °C and 50-500 parts by weight of an energy source for fermentation and adding water for maintaining moisture; performing primary solid fermentation in a sterilized incubator at 10-40 °C for 3-20 days; mixing 100 parts by weight of the hot water extract, 150 parts by weight of a product of primary solid fermentation, 300-800 parts by weight of rice bran, 50-500 parts by weight of the energy source, and water; and performing secondary solid fermentation in the sterilized incubator at 10-40 °C for 3-20 days.

Description

A composite for a prevention and a removal of disease of plants and a soil conditioning, and manufacturing method of the same

The present invention relates to a composition for preventing and removing pests of various plants, and a method of manufacturing the same. Specifically, a fermentation broth obtained by fermentation by adding rice bran to a hydrothermal extract of plants or mushrooms, such as various trees or herbs, is used as an active ingredient. The present invention relates to a natural composition and a method for producing the same, which are environmentally friendly and harmless to humans, and which have an excellent effect on soil improvement, soil restoration, and prevention and removal of pathogens of plants.

In the cultivation of plants such as crops, the dominant environmental factors, as well as favorable natural conditions such as weather, precipitation and insolation, act largely to inhibit various pathogens.

Among the above biological growth requirements, various pests and pathogens are acting as the biggest factor to hinder the growth of plants, and many chemical pesticides and natural pathogen formulations have been introduced and developed to solve them.

Recently, natural pests and pathogen removal compositions extracted from natural substances have been actively developed due to the risk of human pesticides and the risk of ecosystem disturbance.

Korean Patent Application Publication No. 10-2012-0061221 "Red, Jimmy, Leather Extract and Uses thereof", Korean Patent Publication No. 10-2012-0052589 "Pest control of plants using lacquer extract", Korean Patent Publication No. 10-2011- 0031411 "herbicide compositions and herbicidal methods", and Patent Publication No. 10-2012-0052590 "Lacquer Extract with High Flavonoid Content and Extraction Methods thereof" disclose such natural pest control agents or methods for their preparation. .

All of the above patents relate to a composition for promoting plant growth, an antimicrobial substance or a herbicide using an extract of various wood components as an active ingredient.

In the present invention, a composition and a method for manufacturing the same are simpler in comparison with the known art and a method for effectively preventing and removing filamentous fungi such as strawberry wither, red pepper anthracnose, and ginseng jaxok disease.

Korean Patent Application Publication No. 10-2012-0061221 "Red, Jimmy, Leather Extract and Its Uses Patent Publication No. 10-2012-0052589 "Pest control of plants using lacquer extract" Publication No. 10-2011-0031411 "herbicidal composition and herbicidal method" Korean Patent Application Publication No. 10-2012-0052590 "Larch Extract with High Flavonoid Content and Its Extraction Method"

It is an object of the present invention to improve the soil quality of plants, to restore contaminated soil, to inhibit plant growth and disease by using hydrothermal extracts of a single component or a combination of various plants or mushrooms and fermentation and culture compositions using the same. To effectively prevent and eliminate pathogens that cause.

In order to achieve the above object, in the present invention, the first step of extracting hot water by heating 300 ~ 1000 parts by weight of water per 100 parts by weight of the raw material of leather or organo wood for 5 to 10 hours; 300 to 800 parts by weight of rice bran dried at -20 to 121 ° C. and 50 to 500 parts by weight of fermentation energy source per 100 parts by weight of the hot water extract solution of the first step were mixed with water to maintain moisture, and then 10 to 40 in a sterilized incubator. A second step of fermenting a primary solid for 3 to 20 days while maintaining a temperature atmosphere in the range of ℃; And 150 parts by weight of the first solid fermented product in the second step, 300 to 800 parts by weight of rice bran, and 50 to 500 parts by weight of fermentation energy source in 100 parts by weight of the hydrothermal extract solution of the first step to maintain moisture. Mixing water and maintaining a temperature atmosphere of 10 ~ 40 ℃ in a sterilized incubator, the third step of the second solid fermentation for 3 to 20 days; composition for preventing, removing and soil restoration of the plant, characterized in that it comprises a We propose a manufacturing method.

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Here, after the addition of 100 parts by weight of the secondary solid fermented product in the third step to 1000 parts by weight of sterilized water shake extraction filtration for 3 to 5 hours in an incubator maintaining a temperature atmosphere of 20 ~ 50 ℃ and then the extract The plated by a predetermined amount on a Petri dish medium and plated by a plate smear method may further comprise a fourth step of obtaining a microbial cells after incubation for 24 hours in an incubator temperature atmosphere of 20 ~ 37 ℃.
And a third step of inoculating each of the cultured microbial cells after the fourth step per 30 ml of autoclaved YM liquid medium and incubated in an incubator.

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On the other hand, the raw materials include oak, elm, ginkgo, buckthorn, cucurbita, willow, ash, cedar, bamboo, rhubarb, oak, albizia julibrissin, hawthorn, rattan, hinoki, oak, White, jujube, elm, cypress, cypress, paulownia, birch, barberry, rhododendron, rosewood, 칡, wolfberry, honeysuckle, ginseng, peony, cheondan, cheonnyeoncho, aloe, triticale, eoseongcho, dogshit One or more selected from mugwort, sesame, rhubarb, rhubarb, Cordyceps sinensis, situation mushroom, ganoderma lucidum mushroom and horseshoe mushroom may be added as raw materials.
According to another aspect of the present invention, a composition for preventing, removing, and restoring pathogens of a plant produced by the above-described manufacturing methods is also provided.

According to the present invention, it is possible to effectively prevent and eliminate diseases such as black root rot, cirrhosis, anthrax, fungus, white pattern disease, fungus, late blight, green blight, diarrhea, and the like. The removal composition is a natural ingredient extracted and fermented from a natural substance, which is harmless to the human body and is environmentally friendly, which is advantageous for the protection of the ecological environment, and is excellent in preventing and removing pests and pathogens.

In particular, since the composition according to the present invention is manufactured in a simple process using natural materials readily available from the surroundings, mass production at low cost is possible, and it is advantageous to be widely distributed to each customer, and consequently contributes to the increase in agricultural production. The advantage is that you can.

1 is a diagram showing the microbial cells isolated after culturing according to the present invention.
Figure 2 is a result showing the ability to prevent and eliminate pathogens of the present invention for various pathogens.
Figure 3 is a photograph showing the comparison between the case of applying the composition of the present invention to the melon plantation and not.
Figure 4 is a photograph showing the comparison between the case of applying the composition of the present invention to red pepper plantations and not.
Figure 5 is a photograph of the comparative cut of the harvest with and without the composition of the present invention applied to the melon plantation.

Hereinafter, the technical idea of the present invention will be described in detail with reference to the accompanying drawings. In the present invention, various kinds of plants or mushrooms are used as a material. For example, the trees in the plant are extracted from hydrothermal water under certain conditions by using the leaves, stems, roots, berries, etc. of the tree, and then the hydrothermal extract liquid is rice bran. ) And a fermentation energy source sucrose (sucrose) is mixed and fermented to obtain a composition for preventing and removing pests and pathogens of plants.

The fermentation in the stomach is called primary solid fermentation, and as a composition for preventing and removing pests and pathogens of plants, the composition which has undergone the primary solid fermentation process can be used as it is.

The product of the primary solid fermentation obtained above may be mixed with a certain amount of rice bran and sucrose as an energy source for fermentation, followed by secondary solid fermentation under certain conditions. Fermentation products obtained through this secondary fermentation process are further improved the prevention and removal of pests and pathogens.

Water was added to the fermentation product which passed through the second solid fermentation, followed by shaking extraction filtration, which was plated on a plate medium by a Streak plate culture method and cultured in an incubator for a predetermined time. It can be used as a pest prevention and elimination agent, in which case the effect of removing pests and pathogens is improved.

After inoculating the microbial cultured cells obtained above in a certain amount in YM liquid medium, the microorganisms obtained by culturing in an incubator for a predetermined time or more can be diluted with water and used for preventing and removing plant pests and pathogens.

Leather, oak, oak, elm, ginkgo, vine, zirconia, willow, ash, cedar, bamboo, yellow bark, oak, albizia julibrissin, linden tree, rattan, hinoki, oak The species which becomes a raw material in the hot water extraction which uses leaves, stems, roots, fruit of raw materials such as Japanese mulberry, jujube tree, elm tree, cypress, cypress, paulownia tree, non-tree, hibiscus, red tree, velvet You can choose a single species of tree, such as a tree or an oak tree, or you can choose to mix several trees.

In addition, the leaves, stems, roots, berries of the trees to be used as the raw material may be used by selecting any one of them, or may be used by mixing at least two or more.

In addition, the leaves, stems, roots, fruits of the tree as a raw material may be used as it is, but if necessary, it is also possible to cut to a suitable length or use in the form of chips or powder.

In addition to the types of wood listed above, the raw material for extracting hot water of the present invention is a plant such as 칡, wolfberry, honeysuckle, wild ginseng, peony, cheondandan, cheonnyeoncho, aloe, three hundred gree, eoseongcho, firefly, sesame, rhubarb, rhubarb, and soybean. Mushrooms such as cordyceps, eggplant mushroom, ganoderma lucidum mushroom and horseshoe mushroom can be used.

In the present invention, sucrose is applied as an energy source for fermentation, but it is also possible to apply other sugars including glucose which is used as an energy source for general fermentation.

◎ Step 1: Hot Water Extraction Step

Leather, oak, oak, elm, ginkgo, vine, zirconia, willow, ash, cedar, bamboo, rhododendron, oak, albizia julibrissin, hawthorn, rattan, hinoki, oak, Leaves of trees, stems, roots, berries or cherries, wolfberry, honeysuckle, red ginseng, peony, peony, juniper, elm, cypress, cypress, paulownia, birch 300 ~ 200 parts by weight of water per 100 parts by weight of any one or two selected from Sokdan, Cheonnyeoncho, Aloe, 300 seconds, Eoseongcho, Firefly, Sesame, Rhubarb, Rhubarb, Soybean, Cordyceps sinensis, Sichuan mushroom, Ganoderma lucidum, Horseshoe mushroom Add 1000 parts by weight and heat for 5-10 hours at 50-120 ℃ to extract hot water.

◎ Stage 2: Primary Solid Fermentation

300 to 800 parts by weight of rice bran dried at -20 to 121 ° C and 50 to 500 parts by weight of sucrose, a fermentation energy source, per 100 parts by weight of hot water extracted extract, and mixed with water to maintain moisture. Fermentation is carried out for 3 to 20 days while maintaining a temperature atmosphere in the range of 10 ~ 40 ℃ in a sterilized incubator (incubator).

At this time, the amount of water added above is added to the hot water extraction solution, the mixture of rice bran and sucrose is not enough to squeeze the water by mixing the hand, but sufficient enough so long as water is present in the mixed dough itself. That is, it is not necessary to be particularly concerned with the high and low amount of water to be added, and it will be sufficient if water or moisture is present in the mixture.

The fermented product through such primary solid fermentation may be used as it is or sprayed or sprayed on plants or crops, or added to soil. However, when the secondary solid fermentation described below will be further improved the effect of preventing and removing pathogens.

◎ Stage 3: Secondary Solid Fermentation

150 parts by weight of the first solid fermented product in step 2, 300 to 800 parts by weight of rice bran and 50 to 500 parts by weight of sucrose were mixed in an appropriate amount of water to maintain moisture. After fermentation for 3 to 20 days while maintaining a temperature atmosphere of 10 ~ 40 ℃ in a sterilized incubator.

The secondary fermentation product produced here can be applied as is or diluted to plants or soil.

◎ Stage 4: Microbial Separation Culture

100 parts by weight of the secondary solid fermented product was added to 1000 parts by weight of sterilized water (D, W), followed by shaking extraction filtration for 3 to 5 hours in an incubator at a temperature of 20 to 50 ° C., followed by extracting the extract. Put a predetermined amount on a Petri dish medium (LB, YM) and plated by a plate plate (streak plate culture method) and incubated for 24 hours in an incubator temperature atmosphere of 20 ~ 37 ℃ to obtain microbial cells.

Separated and cultured microbial cells can be diluted with water and sprayed onto plants and crops, or used for soil improvement or soil restoration.

◎ step 5: liquid culture of isolated microorganism

In a Erlenmeyer flask, 1000 ml of distilled water, 10 g of dextrose, 10 g of yeast extract, 3 g of yeast extract, 3 g of malt extract, and 10 g of peptone were mixed, and the YM liquid medium was mixed with an autoclave. To sterilize at 121 ° C. to prepare a YM liquid medium.

After inoculating each of the microorganisms separated and cultured in the above step 4 per 30 ml of the prepared YM liquid medium, the microorganism was obtained by incubating for 24 hours in an incubator at 37 ° C. temperature atmosphere.

The microorganisms thus obtained were diluted with water and showed excellent effects in improving soil quality, soil restoration, and preventing and removing plant pests and pathogens, even in small amounts, and were observed to improve plant growth.

The composition (culture) obtained in each step above was confirmed to have an excellent effect on filamentous fungi that inhibit the growth of plants and suffer from diseases as follows.

Table 1: The fermentation composition of the present invention To filamentous fungi  Minimum inhibitory concentration    Fungus      Fermentation Composition Volume (μl) Con 50 100 150 200 250 300  Minimum concentration (μl) Colletotrichum coccodes KACC 40009 * * * - - - - 150 μl Septoria siegesbeckiae KACC 43052 * * * - - - - 150 μl Monosporascus cannonballus KACC 42093 * * * - - - - 150 μl Sclerotinia nivalis KACC 45150 * * * - - - - 150 μl Colletotrichum orbiculare KACC 40808 * * * - - - - 150 μl Colletotrichum gloeosporioides KACC 40003 * * * - - - - 150 μl Fusarium oxysporum f. sp lycopersici KACC 40032 * * * - - - - 150 μl Ralstonia solanacearum KACC 10695 * * * - - - - 150 μl Rhizoctonia solani KACC 40123 * * * - - - - 150 μl Fusarium sp KACC 40050 * * * - - - - 150 μl Phytophthora infestans KACC 43071 * * * - - - - 150 μl Phytophthora capsici Leonian
KACC 40157 * * * - - - - 150 μl

*: Pathogen growth,-: pathogen growth inhibition

(The secondary culture of the present invention paper disc If more than 150μl applied to Filamentous fungi  We can see the effect of eradication)

In Table 1 aboveMonosporascus cannonballus  KACC NO 42093 is a pathogen that causes black root rot of watermelon, melons, melons and cucumbers,Fusarium  sp KACC 40050 causes strawberry wither disease,Colletotrichum acutatum  KACC 40042 causes pepper anthrax,Sclerotinia nivalis    KACC NO 45150 causes fungal diseases of ginseng, cabbage and lettuce,Septoria siegesbeckiae     KACC NO 43052 causes tomato white blightPusarium oxysporum f. splycopersici KACC 40032 causes wilting disease of tomatoes,Colletotrichum gloeosporioides KACC 40003 causes pepper anthrax,Phytophthora infestans KACC 43071 causes tomato plague,Ralstonia solanacearum KACC 10695 causes foot blight,Rhizoctonia solani  KACC 40123 is a pathogen of ginseng apox,Phytophthora capsici Leonian KACC 40157 is a pathogen that causes pepper blight,Colletotrichum orbiculare   KACC 40808 is a pathogen that causes melon and watermelon anthrax.

According to the culture or the culture microorganism of the present invention, all of the filamentous fungi which cause the disease of various plants and crops as described above have been collapsed.

Example

1. Hydrothermal Extraction Steps:

Hot water extract was obtained by adding 600 parts by weight of water to 100 parts by weight of a mixture of leaves, stems, roots and fruit of the leather tree and heating at 80 ° C. for 8 hours.

2. Primary Solid Fermentation Steps:

500 parts by weight of dried rice bran and 200 parts by weight of sucrose were added per 100 parts by weight of hot water extract and mixed with water, followed by fermentation in a sterilized incubator for 10 days in a range of 30 ° C.

3. Secondary Solid Fermentation Steps:

150 parts by weight of the first fermented product was mixed with 100 parts by weight of hot water extract, and 500 parts by weight of rice bran and 100 parts by weight of sucrose were added thereto, mixed with water, and then fermented in a sterilized incubator for 10 days in a sterilized incubator.

4. Microbial isolation culture step:

100 parts by weight of the second fermented fermentate was added to 1000 parts by weight of sterilized water, followed by shaking extraction and filtration for 4 hours in an incubator at 35 ° C. Then, 100 μl of the extract was placed on a Petri dish medium (LB, YM) and plated. The plate was incubated for 24 hours in an incubator at 30 ℃ to obtain microbial cells. Figure 1 shows the microbial cells isolated after the secondary solid fermentation and culture according to the present invention.

5. Isolation of microbial liquid culture:

The YM liquid medium was sterilized at 121 ° C. with a high pressure sterilizer, followed by inoculation of 3 microns of microbial cells separated and cultured per 30 ml of liquid medium, followed by incubation for at least 24 hours in a 37 ° C. temperature atmosphere incubator.

The cultured microorganisms thus obtained were diluted in a large amount of water and acted on the cropland soils.

Figure 2 is a result showing the ability to prevent and eliminate pathogens of the present invention against various pathogens, in which A represents the culture of the present invention, B represents the pathogen.

As shown in the figure, the growth of filamentous fungal pathogens such as KACC 42093, KACC 40050, KACC 40042, KACC 45150, KACC 40808 and KACC 40032 is effectively suppressed.

3 is a photograph showing a comparison result between the case of applying the composition of the present invention to the melon plantation and the other case, Figure 4 is a photograph showing a comparison result between the case of applying the composition of the present invention to the pepper plantation and not.

The illustrated melon plantation and pepper plantation shows the experimental results applied by diluting the culture of the present invention in water and then mixing the soil. As can be seen from the figure, it can be seen that the difference between the plant diseases when the composition of the present invention is applied (the present invention treatment group) and the other case (the present invention non-treatment treatment) is remarkable.

In other words, when the present invention is applied directly to the soil or to the crops, it was confirmed that the disease can be effectively prevented from crops caused by the above-mentioned filamentous fungi.

Figure 5 is a photograph of the comparative cut of the harvest with and without the composition of the present invention applied to the melon plantation. The harvesting season of the melon is usually harvested after about 50 days after flowering, but when the harvesting period of the melon is late, the ovary inside is crushed and it is generally in the same state as the untreated zone of the present invention shown in the right side of the photograph.

By the way, as a result of applying the composition of the present invention, which is the main raw material of the oak tree on the soil of the melon plantation, the inside of the melon is fresh even when the harvest period is over 15 days as shown in the picture on the left. You can see that it stays firm. On the other hand, in the control picture on the right without applying the composition of the present invention, the pulpits are already crushed, and the shape is destroyed, and the pulp is receded.

The present invention relates to a natural soil improvement, soil restoration and pathogen inhibitors and methods for producing the same for effectively preventing, inhibiting and eliminating pests and pathogens of plants. The present invention provides a low production cost, a simple manufacturing process, and an immediate effect. It is necessary to spread it widely as a preventive and exterminator for flowers, forestry and agricultural pests and pathogens.

Claims (7)

A first step of extracting hot water by heating 300 to 1000 parts by weight of water per 100 parts by weight of raw materials of leather or organo wood and heating at 50 to 120 ° C. for 5 to 10 hours;
300 to 800 parts by weight of rice bran dried at -20 to 121 ° C. and 50 to 500 parts by weight of fermentation energy source per 100 parts by weight of the hot water extract solution of the first step were mixed with water to maintain moisture, and then 10 to 40 in a sterilized incubator. A second step of fermenting a primary solid for 3 to 20 days while maintaining a temperature atmosphere in the range of ℃; And
150 parts by weight of the first solid fermentation product in the second step, 300 to 800 parts by weight of rice bran, and 50 to 500 parts by weight of fermentation energy source in 100 parts by weight of the hot water extract solution of the first step were added to maintain water. After mixing the third step of the second solid fermentation for 3 to 20 days while maintaining a temperature atmosphere of 10 ~ 40 ℃ in a sterilized incubator;
Pathogen prevention, removal and soil restoration composition production method of a plant comprising a. The method of claim 1,
After adding 100 parts by weight of the secondary solid fermented product in the third step to 1000 parts by weight of sterilized water, the mixture was shaken and filtered for 3 to 5 hours in an incubator maintaining a temperature atmosphere of 20 to 50 ° C, and then the extract was petri. Putting a predetermined amount on a plate medium, plated by a plate smear method, and cultured for 24 hours in an incubator temperature atmosphere of 20 ~ 37 ℃ and further comprising the fourth step of obtaining microbial cells, pathogen prevention, removal and soil of the plant, characterized in that Method for preparing a composition for restoration. The method of claim 2,
The fifth step of inoculating three hundred platinum inoculated with the cultured microbial cells after the fourth step per 30 ml of autoclaved YM liquid medium and incubated in an incubator
Pathogen prevention, removal and soil restoration composition manufacturing method of the plant, characterized in that it further comprises. 4. The method according to any one of claims 1 to 3,
In the raw materials
Oak, elm, ginkgo, vinewood, custard, willow, ash, cedar, bamboo, rhubarb, oak, albizia julibrissin, linden tree, wisteria, hawthorn, oak, oak, jujube, Arbor, cypress, cypress, paulownia, noncissus, hibiscus, redwood, velvet, 칡, wolfberry, honeysuckle, ginseng, peony, celestial herb, cheonnyeoncho, aloe, triticale, eoseongcho, firewood, sesame, Method for producing a composition for preventing, removing and restoring soil pathogens, characterized in that any one or more selected from rhubarb, rhubarb, Cordyceps sinensis mushroom, Ganoderma lucidum mushroom and horseshoe mushroom are added as raw materials. A composition for preventing, removing, and restoring pathogens of plants produced by the method of any one of claims 1 to 3. delete delete

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