EA029212B1 - Biologically active synergetic composition - Google Patents

Abstract

The invention is related to bioactive compositions produced from natural products of vegetable origin, and can be used for ecological arable farming, in particular, to means for presowing seed treatment and for postemergence treatment of vegetating plants. The invention is directed to creation of a new preparation based on a combination of natural flavonoids, triterpenoids and lignin combined with auxiliary substances allowable for organic agriculture in respect of different crops. The technical result is attained by provision of water emulsion of biologically active synergetic composition including a combination of bioflavonoids with lignin in the synergetic ratio of components of 1.0:0.19-0.27 by mass, an organic solvent and a natural emulsifier, the content of substances in the water phase of the emulsion being as follows: arabinogalactan 12-17% g/l, dihydroquercetin 0.23-0.28 wt.%, and water-soluble triterpenoids at the weight ratio of 0.011-0.09 wt.%. It is advantageous also to use, as a natural emulsifier, lecitin in the amount of 6-9% of the emulsion volume. The biologically active synergetic composition is used by preparation of a working solution by way of diluting the emulsion in water followed by application of the emulsion to surfaces of agricultural crop seeds or spraying onto surfaces of vegetating cultural plants.

Description

The invention relates to bioactive compositions obtained from natural products of plant origin, for ecological farming, in particular to the means for pre-sowing seed treatment and post-emergence treatment of vegetative plants. The invention is directed to the creation of a new biological product on the basis of a combination of natural flavonoids, triterpenoids and lignin in combination with auxiliary substances acceptable for the needs of organic farming in relation to various cultures. The technical result is achieved by the fact that the aqueous emulsion of the biologically active synergistic composition includes a complex of bioflavonoids with lignin in a synergistic ratio by weight of components 1.0: 0.19-0.27, an organic solvent and a natural emulsifier with an arabinogalactan 12- in the aqueous phase 17% g / l, dihydroquercetin 0.23-0.28 wt.% And water-soluble triterpenoids in a weight ratio of 0.011-0.09 wt.%. It is also advisable to use lecithin in the form of an oil solution, taken in the amount of 6-9% of the emulsion volume, as a natural emulsifier. The biologically active synergistic composition is used by preparing a working solution by diluting the emulsion in water, followed by applying it to the surface of crop seeds or spraying vegetative cultivated plants on the surface.

029212 Β1

029212

The invention relates to bioactive compositions obtained from natural products of plant origin, for ecological farming, in particular to the means for pre-sowing seed treatment and post-emergence treatment of vegetative plants.

Prior art

The high biological activity of flavonoids emitted from waste products from plant raw materials, such as birch bark, butt part of Siberian and European larch, grape seed, etc., is known as a regulator of auxin transport - plant hormones that control the growth and development of plants. Also known are antibacterial and antifungal properties of bioflavonoids that help protect plants from pathogens of various infectious diseases, as well as the ability to protect plants from environmental stress, resulting in the formation of free radicals that disrupt cell vital processes [1].

Plant triterpene glycosides, powerful immunomodulators, have a wide range of biological activity no less than flavonoid preparations. The mechanism of their action is that it causes the expression of genes responsible for immunity, and activates the enzymes and reactions necessary for the synthesis of chlorophyll and the light reaction of photosynthesis. An important chemical property of triterpenoids is the ability to form complexes with phenols, including flavonoids, as well as higher alcohols [2].

The high biological activity of larch lignin is known, in particular its antioxidant properties. In the patent of the Russian Federation [3] the values of the antioxidant activity of lignins isolated from various plant materials, in particular larch, are given - 45.8 ± 2.4 AOE, cC / 100 g. Lignin is a source of biologically active substances with a very diverse set of useful properties for use in medicine, veterinary medicine, plant growing, cosmetology [4]. Unfortunately, in both papers, there is not enough data on the biological activity of lignin relative to crops, the data on the biological activity of lignin are only related to its water-soluble forms.

Thus, the task of studying the biological activity of combinations of representatives of the classes of flavonoids, triterpenoids and water-insoluble lignins, aimed at creating new drugs that have significantly higher activity against various biological objects, is relevant.

A known group of preparations is in the form of emulsions [5], the microcapsules of which contain in the oil phase one or several biologically active substances isolated from fish oil, linseed oil, omega-3, and / or omega-6, and / or omega-9 fatty acids , conjugated linoleic acid, terpenoids, carotenoids, tocotrienols, flavonoids, hormones, antioxidants. The method of obtaining such preparations is based on the interfacial polymerization of emulsions, in which viscosity modifiers and emulsifiers use polysorbates, and an emulsifier based on soy lecithin is preferred as the primary one.

The advantages of the known invention is its use in the production of food for humans (especially for the production of dietary supplements) and animals, including fish, as well as microorganisms. In addition, the preparations can be applied for other purposes, in particular for cosmetics, encapsulation of attractants, sterilizers, repellents, insecticides, herbicides, fungicides, bactericides, antiviral drugs, as well as for gene therapy and other similar purposes.

Unfortunately, the materials of the invention did not disclose any formulations related to preparations involving combinations of biologically active substances, nor methods for studying their activity, or methods for their preparation. Also, there are no data related to drugs and methods for their production in order to protect plants from the harmful effects of the environment, strengthen their root systems, stems, stems, leaves, flowers and fruits, as well as data on the effect of drugs on the size and quality of the crop .

Known compositions of nanoemulsions with biologically active compounds, which are used as bioflavonoids: rutin, quercetin, dihydroquercetin and triterpenoid betulin [6]. The known nano-emulsion contains 35-80% of the hydrophobic phase, 17-43% of surface-active substances, 37% of cosolvents and 1-15% of the aqueous phase, where mixtures of mono-, di-, and triglycerides with saturated mono- and diesters are used as the hydrophobic phase. unsaturated fatty acids, and surfactants from the group of sorbitans - mixed with polyhydroxyalkanes or monohydric alcohols.

The advantages of the known composition of nano-emulsions are the ease of preparation, the possibility of using as active elements polyphenolic, triterpene compounds, vitamins and microelements, as well as obtaining formulations with a prolonged release of hydrophilic active ingredients. Unfortunately, in the materials provided there are no data on the physicochemical properties and biological activity of the composition of nanoemulsions with the combined use of the active ingredients.

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There is a method of regulating the growth of grain crops, including the pre-sowing treatment of seeds and vegetative plants with the drug "Larixin", which is a 5% aqueous emulsion with the content of the active substance in a concentration of 50 mg / ml, while the pre-sowing treatment of seeds is carried out at a consumption rate 100-250 ml per 1 ton of seeds, and the treatment of vegetative plants is carried out twice in the phase of the beginning of the release into the tube and in the phase of appearance of the flag leaf at the consumption rate of the drug 30-75 mg / ha [7].

Due to the main active ingredient dihydroquercetin, the commercial drug Larixin (Research Institute Biochemical Protection, ZAO Ametis) can enhance the resistance of cultivated plants to diseases, pests and adverse climatic conditions, increases (induces) in cultivated plants, the expression (activity) of protection genes . In addition, it has a stimulating effect on the immune system of plants, preventing and reducing to a large extent the damage to plants by fungal and bacterial diseases. Impact on a plant with a biologically active substance leads to an increase in the activity of stress-resistance genes, thereby the plant synthesizes special substances whose function is the organization of the connection between environmental factors and the activity of individual genes or their blocks. The use of the drug "Larixin" allows you to minimize the need for treatment of crops with fungicides, as well as increase crop yields [8]. Among the significant drawbacks of the known drug are the problems associated with its practical application at the stage of preparing working solutions and their application to the surface of vegetative plants. Also, there is no data on the formulation of the drug in terms of excipients and the possibility of its use for the purposes of organic farming.

The share of organic agriculture tends to grow in most European countries. In recent years, the growth in demand for agricultural products produced as part of the reform of the common agricultural policy, which is aimed at the development of organic farming, is particularly noteworthy [9]. This has developed a regulatory framework governing the organic sector [10].

In this regard, preference is given to the use of preventive measures to control pests, diseases and weeds. The use of pesticides, which can be detrimental to the environment or present as residues in agricultural products, is severely limited. Also significantly limited the list of biologically active and auxiliary substances that can be used as fertilizers and plant protection products. Thus, the task of developing new biologically active drugs, in the formulations of which can be used active and auxiliary substances suitable for use in organic farming, is relevant.

A biologically active synergistic composition is known, the aqueous emulsion of which includes the bioflavonoid complex of dihydroquercetin, aromadendrin and naringenin, which are taken in a molar ratio of 1.0: 0.102-0.104: 0.014-0.016, an organic solvent and a natural emulsifier. In addition, arabinogalactan and water-soluble triterpenoids are introduced into the aqueous phase of the emulsion in a weight ratio of 1-3: 5-7 in an amount of 0.3-0.7 wt.% Of the aqueous phase, and the bioflavonoid complex is distributed between the oil and water phases in a weight ratio 5-10: 1 [11].

Commercial preparations "Agrostimul" and "KoYrtad" (Agruskhim LLC, IAV "Koktkky rgaschsbgiIaG") [12], developed for organic farming, are highly effective biological stimulators of the growth and development of plants for processing grain, vegetable, industrial, flower and decorative crops The use of drugs contributes to an increase in seed germination energy and the intensity of root development, increases the resistance of crops to various stresses (lack of moisture, frost damage, mechanical damage, treatment of herbicides, etc.), inhibits the development of some fungal and bacterial infections, helps to accelerate the maturation and the onset of biological and technological maturity, increases the yield and product quality.

Among the significant shortcomings of both drugs should be attributed to the high sensitivity of vegetative plants to overdose of the drug, which in some cases leads to intensive development of root systems with a slight increase in the growth of the aerial parts of plants. In addition, during long-term storage of drugs at the bottom of the container, a thickening of the emulsion is observed, which leads to the need for intensive agitation before use.

The aim of the invention is to create a new biological product on the basis of a combination of natural flavonoids, triterpenoids and lignin in combination with auxiliary substances acceptable for the needs of organic farming in relation to various cultures.

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DISCLOSURE OF INVENTION

This goal is achieved in that the aqueous emulsion of the biologically active synergistic composition includes a complex of bioflavonoids with lignin in a synergistic ratio by weight of components 1.0: 0.19-0.27, an organic solvent and a natural emulsifier with an arabinogalactan content of 12-17 in the aqueous phase of the emulsion. % g / l, dihydroquercetin 0.23-0.28 wt.% and water-soluble triterpenoids in a weight ratio of 0.011-0.09 wt.%.

In a biologically active synergistic composition, it is advisable to use lecithin in the form of an oil solution, taken in an amount of 6–9% by volume of the emulsion, as a natural emulsifier.

Another object of the invention is a method of applying a biologically active synergistic composition by preparing a working solution by diluting the emulsion in water, followed by applying it to the surface of crop seeds or spraying vegetative cultivated plants on the surface.

The biological activity of the compositions of different ratios of active ingredients of the studied compounds was carried out in the laboratory of physico-chemical analysis, laboratory of artificial climate and in the field.

In terms of the physicochemical laboratory, studies were conducted on the antiradical activity of the bioflavonoid complex and water-insoluble lignin, such as dihydroquercetin (IT) with a purity of 99.9%, aromadendrin (ΆΚΌ) with a purity of 98.9% and naringenin (ΝΑΚ.) 97.5%, and also their various combinations with a water-insoluble lignin, an amperometric method using a TsvetYauza 1-A chromatograph with an amperometric gold-plated detector (Scientific-Production Association "Himavtomatika"). Antiradical activity was measured relative to the standard - Trolox in units of OC.AC yubgo ualie (tct1 TE / d). The active ingredients were isolated in accordance with the invention, “A method for the preparation of highly purified dihydroquercetin” (“Pröezz £ og Prépärpd à £ ЫDiu riga bSubgodiegeyp”) by the method of preparative chromatography on a column ной 100-700 with dynamic axial compression filled with phase sorbent with increased retention of polar compounds with a particle size of 10-20 microns and a pore size of 120-200 A.

Quantitative analysis bioflavonoid complex and its active components was made by HPLC on a chromatograph mass spectrometer "8YtaSchts №hega 2020" under the following conditions: column (NRBS So1itp) - 250x4,0 mm, reversed-phase sorbent - YMC-Rask ΟΌ8-ΑΡ Cx ₈ , Ι.ϋ. 8 - 5 μm, the analytical wavelength - 290 nm, the mobile phase - acetonitrile in 0.1% aqueous solution of acetic acid (25:75), the injection volume (Euéciente Voyte) - 10 μl, the flow rate of the mobile phase - 80 μl / min, chromatogram recording time - 25 min. The processing of the results was carried out using the EA8o1i1uppz BSM8 program.

Based on the data on the effectiveness of composite preparations, which include more than one active substance, the nature of the biological interaction of the active components that make up the composition is calculated to assess the level of their synergism, antagonism or additivity, i.e. simple addition of the effects of each of the components of the mixture preparative forms.

In herbology, it is customary to evaluate the nature of biological interaction using mathematical dependencies proposed by Kolby and Limel. In accordance with Kolbie Limpela’s computational formulas for blending, for comparison, select the efficacy data of the individual preparations applied separately in a specific dosage and the effectiveness of the mixture composition, where the constituent components are taken in the same dosages or very close to them.

The synergistic effect of the study drugs was calculated from formula (1) Colby (see So1u 8.K .. Sa1si1aypd 8upeg§1zys APB Ap1adotzys gezropzez about £ yegYshbe sotYpayop ^\\;. EEC) of 1967, V. 15, № 1, p. 2022):

E = EE-E ₀ (1),

where E is the synergistic effect of the composition;

E _e - the value of the result in the experiment;

E _o - the expected value of the result in the experiment.

An example of calculation for a three-component composition (A + B + C):

E = x + y + g—

xy + χζ + γζ

100

hug

10,000 ’

where E is the "expected" effect of the composite drug (A + B + C); x - the value of the result from the use of component A individually at a dosage close to its content in the composition; y - the value of the result from the use of component B in a manner similar to the previous one; ζ - value of the result from the use of component C in the same order.

When the actual effect of a mixture of 2 or more substances is significantly higher than the "expected" E _o calculated by the formulas, then we can talk about the synergism of the components of the composition in the nature of the biological interaction; if the actual effect obtained in the experiment is noticeably lower than the "expected" E _o , then antagonism of the components of the mixture is observed; in the case of the “expected” E _o , coinciding - 3 029212

With the result of the actual experimental value, one can judge the additivity of the interaction of the components of the mixture or composition.

Studies have shown that all three components of the bioflavonoid complex dihydroquercetin, aromadendrin and naringenin, both in pure form and in compositions, exhibit high biological (antioxidant) activity. The effect of different ratios of dihydroquercetin, aromadendrin and naringenin on the level of biological (antiradical) activity is illustrated in Table. one.

Table 1

Molar an attitude IT / AKO / ΝΑΚ Dose components in the complex (§ / 1) Antioxidant activity according to OKAS (tcto! TE / e). ϋΗΟ ACT) ΝΑΚ Ee Yeo E 1: 0: 0 50 0 0 25.40 0: 1: 0 0 five 0 9.70 0: 0: 1 0 0 0.7 7.90 0.15: 0.01: 0.002 45.638 2,883 0.545 17.30 11.88 5,423 0.16: 0.01: 0.002 48.68 2,883 0.545 18.80 12.65 6.1527 0.17: 0.01: 0.002 51,723 2,883 0.545 20.50 13.42 7.0824 0.16: 0.015: 0.002 51,723 4.324 0.572 23.70 13.54 10,159 0.16: 0.016: 0.002 51,723 4,612 0.545 24.20 13.56 10,637 0.16: 0.017: 0.002 51,723 4,900 0.545 28.80 13.59 ' 15.212 0.165: 0.017: 0.0025 50,201 4,900 0.681 29.70 13.21 16,488 0.16: 0.015: 0.001 51,723 4,612 0.272 19.90 13.54 6.3553 0.16: 0.016: 0.002 51,723 4,612 0.545 24.20 13.56 10,637 0.16: 0.017: 0.003 51,723 4,612 0.817 26.50 13.58 12,918

As the data table. 1, the optimal ratio of the components of the studied compositions exhibiting maximum antioxidant activity should be considered the composition of the composition: dihydroquercetin - 0.165 mol / l, aromadendrin - 0.017 mol / l and naringenin - 0.0025 mol / l, which corresponds to the molar ratio of the components studied 1.0 : 0.102-0.104: 0.014-0.016. In the field of optimal molar ratios, the studied bioflavonoid complex exhibits maximum biological activity with a synergistic effect of 12.9-16.5%.

The effect of different ratios of the bioflavonoid complex of the CTC composition dihydroquercetin 0.165 mol / l, aromadendrin - 0.017 mol / l and naringenin - 0.0025 mol / l with water-insoluble lignin ΥΝΕ on the level of biological (anti-radical) activity is illustrated in Table 2

table 2

Weight ratio VRK / UZH The dose of the components in the composition (§ / 1) Antioxidant activity according to OKAS (tcto! TE / §). VEK UZH Ee Yeo E% ten 50 0 29.7 0: 1 0 five 9.7 1: 0.15 45.6 6.8 36.8 36.8 0.0 1: 0.17 51.7 8,8 42.6 42.5 OD 1: 0.19 51.7 9.8 48.1 43.9 4.2 1: 0.20 51.7 10.3 49.3 44.6 4.7 1: 0.21 50.2 10.5 50.1 44.2 5.9 1: 0.22 51.7 P, 4 50.7 46.0 4.7 1: 0.23 51.7 And, 9 51.2 46.7 4.5 1: 0.24 51.7 12.4 51.3 47.4 3.9 1: 0.25 50.2 12.6 51.1 46.9 4.2 1: 0.26 50 13.0 51.2 47.4 3.8 1: 0.27 51 13.8 51.4 48.9 2.5 1: 0.28 50.2 14.1 49.4 49.0 0.4 1: 0.29 50.1 14.5 49,6 49,6 0.0

As it follows from table. 2, the optimal ratio of the components of the studied compositions exhibiting maximum antioxidant activity should be considered the composition of the composition of the bioflavonoid complex - 50.2 g / l: water-insoluble lignin - 10.5 g / l, which corresponds to the ratio of the studied components 1.0: 0.021. In the field of optimal molar ratios of 1: 0.19-0.27 investigated

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the composition exhibits maximum biological activity with a synergistic effect of 2.5-5.9%. Biologically active synergistic composition in the form of an aqueous emulsion contains components in the following weight ratios:

Bioflavonoid complex composition: (Dihydroquercetin (Takhoyupe) SAZ 207-543-4, Aromadendrin (O1BuigokaetrGego1) SAZ 480-20-6, Naringenin (№pp§eshp) SAZ 480-41-1) EO Lignin SAZ 9005-53-2 0,19-0,27 Surfactants, (8GGas1ap1z) May. % 6-9 Solvent (δοϊνβηί), May. % 7-9 Water rest up to 100

Below are the characteristics of surfactants and solvents that can be used in a new biologically active synergistic composition, which is conventionally called "Aleox".

Besyp Zawa BesShpp Yytshy SAZ 8002-43-5 AgaPo§a1ac1ap Ro-wagons of phoaa1ac1ap Ggot LabsB U / oos1 SAZ 9036-66-2 δοϊνεηί Rgoru1epe §1uso1 SAZ 57-55-6

The main problem in emulsion technology is to ensure physical stability. The following types of instability are characteristic of emulsions - dispersed systems with a developed interfacial surface and having an excess of free surface energy: the thermodynamic ability to maintain in time the constant sizes of the dispersed phase droplets and the sedimentation ability in time to maintain a constant distribution of the dispersed phase droplets throughout the system.

The method for determining sedimentation stability is based on the separation of the emulsion into oil and water phases during centrifugation.

Two tubes were filled with half the volume of the test emulsion and weighed, the result was recorded to the second decimal place. The mass difference of the emulsion tubes should not exceed 0.2 g. The tubes were placed in a water bath and kept for 20 minutes at a temperature of 22-25 ° C, removed from the water, wiped dry from the outside and placed in the centrifuge slots. Centrifugation was carried out for 5 min at a frequency of rotation of 1000 rpm, then the tubes were removed and the stability of the emulsion was determined. If in one of the tubes a separation of the emulsion was observed, then the test was repeated with new portions of the emulsion.

In the absence of a clear separation of the emulsion, the contents of the tube were carefully poured onto a sheet of white thick paper and the presence or absence of separation of the emulsion was noted. The emulsion was considered stable if, after centrifuging in test tubes, no more than a drop of the aqueous phase or oil layer was noted to be no more than 5 mm.

The method of determining thermal stability is based on the separation of the emulsion into oil and water phases at elevated temperatures.

Three test tubes with a diameter of 14 mm and a height of 120 mm or cylinders with a capacity of 25 cm ^{3 are} filled to half the volume with the test emulsion, ensuring that no air bubbles remain in the emulsion, are closed with stoppers and placed in a thermostat with a temperature of 40-42 ° C. When determining the thermal stability of the emulsion, the contents of the tubes or cylinders after 1 h of thermostating were gently stirred with a glass rod to remove air. The emulsions were incubated for 24 h and then the stability was determined. The emulsion was considered stable if, after incubating in test tubes, no precipitation of the aqueous phase or discharge of the oil phase layer was observed to be no more than 5 mm.

Thermodynamic stability was determined by the method of determining the stability of the emulsion in accordance with the patent of the Russian Federation No. 2464970, including sampling and sample preparation, as well as in determining sedimentation stability. Then there was a microscopic observation of the emulsion under study and counting the number of micelles on the 1 cm ² site at 2000 times magnification, followed by comparison with the number of micelles η in the sample not subjected to preparation, considering the emulsion to be conditionally stable at η / ηο · 100> 50. Then, samples with a conditionally stable emulsion were subjected to accelerated aging for a month in a thermostat at a temperature of 40-42 ° C and a relative humidity of 65 ± 2%, periodically conducting microscopic observation of samples on an area of 1 cm ² at 2000x magnification. Statistical processing of the experimental data was carried out according to the equation η '= a exp (a1-1 / T), where η' is the number of micelles, pieces / cm ² ; ι - time of accelerated aging, days; a, a ₁ , are the calculated coefficients of the exponential equation obtained by processing the experimental data, with T ^ given

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storage time, with the calculation of the possible state of the emulsion when the value of p / p ₀ -100> 50 emulsion is considered stable and suitable for long-term storage.

Initially, tests on the sedimentation and thermodynamic stability were subjected to two samples of biologically active synergistic composition (standard) "Agrostimul" and "Aleks". Samples were obtained with various combinations of surfactants: the Agrostimul sample contained 120 ml / l of a natural emulsifier for an oil solution of soy lecithin, the sample of Aleox contained 90 ml / l of a natural emulsifier of an oil solution of soy lecithin. In both cases, a solvent was used - propylene glycol in an amount sufficient to completely dissolve the bioflavonoid complex and water-insoluble lignin. Both samples showed the same sedimentation stability — no separation of the emulsion was observed. Both samples demonstrated a high thermodynamic stability - the criterion value p / p _of -100 to sample "Agrostimul" criterion value after 24 hours n / n _about -100 was 97% and for sample "Aleoks" - 98%, and after 24 h 95 , 5 and 97.5% respectively.

Quite unexpectedly, the authors found that during prolonged storage, more than a year, at rest, in the bottom layer of the samples of both preparations, a more dense phase was observed, and the separation of the oil and water phases was not observed. Moreover, the chemical composition of drugs in the thickened phase and in the rest of the volume remained the same. The authors associated the observed phenomenon with a significant difference in the size of emulsion micelles associated with insufficient viscosity of the aqueous phase of the emulsion.

Viscosity determination was carried out on a Brookfield EU-E rotational viscometer using a measuring cylinder 5, at a fixed temperature of 25 ° C.

In tab. 3 illustrates the effect of the concentration of arabinogalactan in the emulsion of the biologically active synergistic composition “Aleox”, as well as viscosity, mixing power, circulation ratio on the average micelle size and stability of the preparation.

Table 3

00raztsa Concentration arabinogalactan, g / l The viscosity of the emulsion, MPa * s The average size of micelles, microns Stability drug % P / US ² one ten 177.5 4.2 96.7 33 2 12 178.0 1.2 98.2 39 3 14 178.7 0.9 98.4 39 four sixteen 179.5 0.22 98.5 39 five 18 180.0 0.13 99.1 40 6 20 184.5 0.12 95.1 29 7 22 185.5 0.11 94.1 27

As it follows from table. 3, the highest sedimentation and thermodynamic stability was demonstrated by samples from No. 2 - 98.2% to No. 5 - 99.1%. Also, the best result was demonstrated by samples 2 through 6 by microscopic examination of the average number of drops of working solutions of both samples by diluting the original emulsions 100 times at 2,000 times magnification - 39 drops in 1 cm and 40 drops in 1 cm, respectively. A similar result was demonstrated by samples from No. 2 to 5 and from the point of view of the size of micelles - from 1.2 to 0.13 microns. A completely unexpected result was obtained by increasing the concentration of arabinogalactan above 18 g / l. The increase in emulsion viscosity is higher than 180 mPa-s with a significant increase in mixing power to 0.075 kW / l and a decrease in the average size of micelles below 0.12 μm, resulting in a decrease in the stability of the preparation to 94.1% and the number of drops to 27.

In the vegetative experience, the primary assessment of the phytotoxicity of the drug "Aleox" was carried out in the laboratory of artificial climate (VCI) under the following camera operation modes: illumination of 20 thousand lux for 14 hours, without illumination - 10 hours a day, daytime air temperature is 18 ° C, at night 12 ° С, relative humidity of air 70%, soil moisture in vegetation vessels 60% of PV, maintained by daily watering by weight with desalted water. As a test plant, watercress was used (Eozrhbsh zayuish E.) of the variety "KaY1a1a" - an annual, herbal, cold-resistant, early-ripening variety. The period from germination to technical ripeness 20-25 days. Watercress has an increased sensitivity to environmental pollution and is distinguished by rapid germination of seeds, almost 100% germination, which decreases markedly with harmful effects, the shoots and roots of the plant undergo noticeable morphological changes: growth retardation and curvature of the shoots, reduction of the length and weight of the roots , as well as the number and weight of seeds.

The experiment was carried out with the germination of seeds of the watercress test plant under the conditions of the above regimes using single-use vegetation vessels with a capacity of 600 g of a mixture of sod-podzolic soil, sand and organic matter in a ratio of 1: 1: 1. In each vessel, 20 treated seeds of the test plant were left. The experiment was carried out in triplicate, with the release of four experimental on working solutions of samples No. 2 and 5 of the table. 3, and one control group per

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drinking water. The number of seeds treated in each group - 20 pcs. All seeds were taken from the same batch.

Working solutions for soaking seeds were prepared by diluting the original emulsions 10, 100 and 200 times. The seed soaking of the test plants was carried out for 2 hours, after which the treated and control seeds were transferred to the vegetation vessels.

Biological activity was evaluated every 8 hours, starting from the second day after treatment, in comparison with the untreated control. On the 5th and 10th day, the results were evaluated by the difference in the wet weight of the test plants. The results of the initial assessment of phytotoxicity of the drug "Aleox" in relation to cress varieties "Kasa1a" are given in table. four.

Table 4

5 days 10 days 15 days Mass, g %to control Mass, g %to control Mass, g %to control Sample No. 2, a solution of 0.5% 17.7 118.0 26.4 105.6 32 106.7 Sample No. 2, a solution of 1% 11.5 76.7 19.8 79.2 29.8 99.3 Sample No. 2, a solution of 10% 7.9 52.7 14.4 57.6 24.4 81.3 Sample No. 5, a solution of 0.5% 19.3 128.7 27.3 109.2 34.7 115.7 Sample No. 5, a solution of 1% 13.70 91.3 23.7 94.8 32.2 107.3 Sample No. 5, a solution of 10% 10.9 72.7 19.6 78.4 28.4 94.7 Control 15 25 thirty

As it follows from table. 4, the drug "Aleox" shows phytotoxicity for cress varieties "Kasa-1a" at a concentration of working solutions of samples 2 and 5 - 10%. The working solution of sample No. 2 at a concentration of 1% of phytotoxicity did not show, but also did not show and stimulate plant growth - at the control level. Working solutions of sample No. 5 at a concentration of 0.5 and 1% stimulated the growth of plants by 15.7 and 7.3%, respectively.

Observations also showed that similar results were observed in all three replications. Ontogenesis of the control group was carried out according to the standard scheme. In the first group (10% working solutions of samples 2 and 5), depression of the cotyledon was noted, germination decreased by 5% and the prolongation of the seedling phase, but later the plants developed according to the standard scheme, but with a significant delay. In the second group (10% working solutions of samples 2 and 5), the growth reactions in the first and second days slowed down, but on the third they recovered. Nevertheless, the depression of plants was clearly visible during the first 10 days. In the third group (0.5% working solutions of samples Nos. 5 and 6), an increase in growth reactions was noted during the entire observation time.

Thus, during pre-emergence treatment of cress seeds phytotoxicity of biologically active synergistic composition "Aleox" is noted in the area of working solutions concentrations above 1%, and with a higher degree of dilution, for example, up to 0.5% and higher, stimulation of plant growth is noted. The results of similar experiments to determine the phytotoxicity and effectiveness of working solutions of the biologically active synergistic composition "Aleox" in comparison with the preparation "Agrostimul" (Etalon) during the pre-emergence treatment of seeds of cultivated plants are given in Table. five.

Table 5

Effective concentration of working solutions,% Average efficiency worker solution% "Aleox" "Agrostimul" "Aleox" "Agrostimul" Wheat 0.4 0.5 0.6 0.7 Rye 0.4 0.5 0.7 0.7 Barley 0.4 0.5 0.6 0.7 Buckwheat 0.5 0.7 0.5 0.9 Oats 0.5 0.8 0.9 one Soy 0.75 1.0 1.15 1.35 Rape 1.1 1.2 1,3 1.25 Sunflower 1.75 2.5 2.4 3 Cucumber 0.05 0.1 0.12 0.15 Tomato 0.05 0.1 0.13 0.15 Sugar beet 1.5 2.5 2.7 3

As follows from the data given in table. 5, the average level of effectiveness of the drug "Aleox" is 19.4% higher than that of the drug "Agrostimul". At the same time, the biological activity of the drug "Aleox" exceeds the average biological activity of the drug "Agrostimul" by the criterion of the effective concentration of the working solution by 29.8%.

Biologically active synergistic composition "Aleox" was tested in field plots- 7 029212

experiments on the lands of the Moscow region, Odintsovsky district.

The weather conditions of the 2012 growing season in some cases differed from the average long-term

indicators, standards are given in table. 6

Table 6

Month, kada de- Air temperature, ° С Precipitation, mm Average year 2012 Deviation from the average year Average long-term 2012 Deviation from the average perennial May one 10.7 12.6 +1.9 14.1 21.5 +7.4 2 12.0 16.5 +4.5 19.1 5.9 -13,2 3 13.5 15.2 + 1.7 21.4 9.2 -12,2 the average 12.1 14.8 +2.7 Σ54.6 Σ 36.6 -18,0 June 15.2 13.7 -1.5 24.8 41.1 +16.3 2 16,1 18.5 +2.4 23.4 32,8 +9.4 3 16.8 17.0 +0.2 24.3 25.4 + 1.1 the average 16,0 16.4 +0.4 Σ72.5 Σ 99.3 +26,8 July one 17.2 21.8 +4.6 28.9 0.7 -28,2 2 17,8 18.3 +0.5 28.0 22.9 -5,1 3 17.6 20.4 +2,8 26.2 16,0 -10,2 the average 17.5 20.2 +2.7 Σ83.1 Σ 39.6 -43,5 August one 17.5 21.3 +3.8 22,8 1.7 -20,1 2 15.6 17.2 +1.6 22.5 40.6 +18.1 3 14.6 13.9 -0.7 26.0 32.3 +6.3 the average 15.9 17.3 + 1.4 Σ71.3 Σ76,6 +5.3

As can be seen from the table. 6, the vegetation period of 2012 did not differ significantly from the average annual values in air temperature, but on average May-August were warmer than usual by 0.4-2.7 ° C. Precipitation during the growing season (from May to August) was extremely uneven compared with the average annual levels (the rain deficit ranged from 18.0-43.5 mm by months). So, in May (sowing of soybean) precipitation fell less than 18 mm from the norm, whereas in July (the period of active growth of cultivated and weedy plants) the amount of precipitation exceeded the average long-term indicator by 26 mm. July was relatively dry, the deficit of precipitation was 43 mm, and August in terms of precipitation was rather rainy and did not differ from the average long-term indicators. In general, the growing season of 2012 in terms of temperature and precipitation was quite favorable for the growth and development of both cultivated and weedy plants, and in practical terms did not differ significantly from the average for the conditions of the Moscow region.

Culture: Bar soybeans. Seeding rate: 100 kg / ha. Sowing date: 22.05.2012. Time of emergence: 02.06.2012. Soil of experimental plots: sod-podzolic with a humus content of 2.5%, pH _BO 5.8; CEC 11 mEq / 100 g soil.

On the day of the pre-emergence treatment: 28.05.2012. Air temperature: 15.6 ° С. Relative humidity: 64%. Wind speed: less than 1.8 m / s. The time of precipitation after treatment and their intensity: after 2 h, 1.5 mm.

On the day of the first vegetative processing: June 28, 2012 (phase of the beginning of flowering): air temperature: 18.2 ° C. Relative humidity: 50%. Wind speed: less than 1.8 m / s. Time of precipitation after treatment and their intensity: no.

On the day of the second treatment: July 15, 2012: air temperature: 15.3 ° C. Relative humidity: 75%. Wind speed: less than 1.8 m / s. The time of precipitation after treatment and their intensity: after 2 h, 0.5 mm.

Extreme weather conditions (hail, frost, heavy rain, etc.): not observed.

Different formulations of the biologically active synergistic composition "Aleox" in comparison with the drug "Agrostimul" (Standard) were applied to vegetative plants in the form of an aqueous suspension using an experimental sprayer OD-2 designed by VNIIF (Bulletin of the Russian Agricultural Academy, 1993, No. 3). The consumption rate of the aqueous suspension is 300 l / ha. The area of experimental plots 25-50 m ² , fourfold, the placement of plots is consistent with the elements of randomization.

The doses of drugs used in field plot experiments are shown in Table. 7

Table 7

A drug Dose of the drug ml / ha First treatment Second treatment "Aleox" sample number 2 40 thirty "Aleox" sample number 5 thirty 25 "Agrostimul" 40 thirty Control (without treatment) 0 0

Dates of surveys of the objects under study: July 12 and August 2. Methods of accounting: quantitative and quantitative-weight methods at survey sites of 0.25 m ² , 4 sites on each plot. Method of harvesting and accounting of the harvest: cleaning in sheaves from 1 m ² , ² replicas on each plot. Statistical data processing using analysis of variance.

The period of effective action of drugs: from the time of spraying throughout the growing season of the crop.

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The economic efficiency of tank mixtures of drugs corresponding to the new biologically active synergistic composition "Aleox" in comparison with the standard for pre-emergence application on soybean crops is given in Table. eight.

Table 8

Option of experience Dose, ml / ton of seeds Grain yield, kg / ha Protected yield, t / ha one_ by repetitions Average yield, c / ha I II III IV "Aleox" sample number 2 100 18.4 20.1 22.0 19.8 20.0 N, 9 "Aleox" sample number 5 100 23.5 23.9 22.0 22.7 23.7 15.6 "Agrostimul" 100 15.5 16.3 15.7 14.9 15.6 7.5 Control (without treatment) - 6.0 8,8 10.7 6.8 8.1

The economic efficiency of tank mixtures of drugs corresponding to the new biologically active synergistic composition "Aleox" in comparison with the standard when spraying into the early flowering phase on soybean crops is given in table. 9.

Table 9

Option of experience The dose of drugs in the form of tank mixtures, ml / ha Grain yield, kg / ha Protected yield, centners per hectare by repetitions Average yield, kg / ha I II III IV Aleks # 2 40 20.6 21.6 22.2 21.4 21.4 19.1 Aleks number 5 40 23.5 23.9 22.0 22.7 23.7 20.7 "Agrostimul" (Etalon) 40 21.0 19.2 17.5 19.0 19.2 16.9 Control (without treatment) - 6.0 8,8 10.7 6.8 8.1

As evidenced by the data presented in table. 7-9, the use of biologically active synergistic composition "Aleox" Sample No. 5 with pre-emergence application and spraying treatment at the beginning of flowering phase led to a significant stimulation of plant growth and a significant increase in the yield of soybean cultivar variety "Bar".

Also during the tests, it was found that samples No. 2 and 5 of the Aleox preparation showed a higher biological activity not only compared to the control, but also exceeded the biological activity of the reference drug Agrostimul by 22.4%. The economic efficiency of using both samples of the synergistic composition "Aleox" was higher with foliar treatment than with pre-emergence drugs: 19.1 - 20.7 c / ha protected soybean crop, which significantly exceeded the yield in the plot with the reference drug "Agrostimul" - 16 , 9 kg / ha.

As a result of field plot studies, it was found that the biologically active synergistic composition "Aleox" steadily shows a tendency to increase economic efficiency when stimulating soybean crops at various doses of use, as well as in all the vegetation phases studied.

Thus, it is advisable to formulate a new biologically active synergistic composition in the form of an aqueous emulsion of the oil-in-water type with a mass synergistic ratio of the complex of bioflavonoids with lignin 1.0: 0.19-0.27, using an organic solvent and a natural emulsifier, with the content in the aqueous phase of the arabinogalactan emulsion, 12–18 g / l, dihydroquercetin, 0.23–0.28 wt.% and water-soluble triterpenoids in a weight ratio of 0.011–0.09 wt.%.

The best uses of the invention.

Example 1

In a heated reactor with a stirrer with a capacity of 0.5 kW, a working volume of 0.3 m ^3, 10.5 liters of propylene glycol SAZ 57-55-6 are loaded and heated to a temperature of 37 ° C, after which 1490 g of dihydroquercetin SAZ 207-543-4 are metered, 130 g of aromadendrin SAZ 480-20-6, 63 g of naringenin SAZ 480-41-1 and 283 g of lignin SAZ 9005-53-2 and stirred at 39 ° C for 45 minutes until completely dissolved. Then, 2745 ml of natural emulsifier SAZ 8002-43-5 soybean lecithin oil emulsifier is metered into the reactor and stirred for 60 minutes. 26 liters of distilled water are poured into a similar reactor and heated to a temperature of 90 ° C, after which 468 g of arabinogalactan SAZ 9036-66-2 and 25 g of water-soluble triterpenoids are added and mixed for 4 hours, after cooling the solution to a temperature of 30 ° C, 145 are added g dihydroquercetin SAZ 207-543-4. Stirring is carried out for 120 minutes at a temperature of 30 ° C. In the first reactor with the prepared oil phase at a rate of 0.3 l / min, for 90 minutes, the aqueous phase is metered at a mixing power of 0.5 kW / l and circulation ratio of 18 units of reactor volume per hour at 35 ° C. The resulting emulsion in the amount of 30.5 l is cooled with vigorous stirring for 60 minutes to a temperature of 20 ° C, and then unloaded from the reactor and poured into 1-liter cans. The content of active com- 9 029212

Ponents: dihydroquercetin - 50.2 g / l, aromadendrin - 4.9 g / l, naringenin - 0.7 g / l, lignin - 9.3 g / l, arabinogalactan - 15.4 g / l, triterpenoids - 6 76 g / l. The particle diameter (50% of the composition) is 0.9 microns. The pH value of the drug is 5.1. The drug is stable in the original packaging for 2 years from the date of manufacture in the temperature range from 30 to -20 ° C. At temperatures below -20 ° C freezes throughout the volume. After thawing from above, the formation of a layer of the aqueous phase with a height of about 5 mm is observed, which disappears with stirring.

Aqueous emulsion of biologically active synergistic composition "Aleox" at a dose of 0.06 l / ha is used by continuous ground spraying of vegetative plants of spring barley varieties Moskovsky 2 in the phase of the beginning of the release into the tube and in the phase of appearance of the flag leaf. The equipment used is the Agrotop knapsack pneumatic sprayer, equipped with a two-meter rod. Working fluid consumption: 300 l / ha.

A comparative assessment of the biological efficacy of the drug "Aleox" carried out in the conditions of the Ryazan region showed high efficiency. Accounting conducted 30 days after spraying of spring barley in the flag leaf phase showed a significant growth ahead compared with the control. Analysis of the elements of the structure of the harvest showed that the formation of the yield of spring barley was mainly influenced by such an element of the structure as the mass of 1000 grains. In all variants with the use of the drug "Aleox", the mass of 1000 grains increased by 20% compared with the control.

Spraying of crops in the start-up phase of the culture at a dose of 0.06 l / ha contributed to an increase in the yield of spring barley by 20.3% and in the phase of appearance of the flag leaf l / ha by 21.2% compared to the yield at the untreated control (yield on control - 30.6 c / ha).

Thus, the biologically active synergistic composition "Aleox" has a high economic efficiency in crops of grain crops, significantly increasing their yield, as well as expanding the temperature range of application.

Example 2

Under the conditions of obtaining the aqueous emulsion of the biologically active synergistic composition "Aleox" described in Example 1, field small-plot tests of the aqueous emulsion of the biologically active synergistic composition "Aleox" were carried out in the Ryazan region of the Russian Federation in the northwestern deciduous forest soil-climatic zone of black soil in the period September 2012 - July 2013. Culture - winter wheat variety Moscow 39, seeding rate - 230 kg / ha. Sowing date - September 10, 2010. Soil - leached chernozem, humus content in the arable layer is 4.8%, pH 5.8, potassium and phosphorus is high. The predecessor is the sidereal mustard steam. Tillage - seal the green mass of mustard with a white disc harrow, pre-sowing cultivation, sowing, rolling. The time of germination is September 20, 2010. Fertilizers - spring dressing под _{60 in a} dispersed manner. The plant development phase at the time of treatment (May 27, 2011) is the tillering stage.

During the whole study, extreme weather conditions were observed - lack of moisture and high temperatures in May-July.

On the day of treatment, the air temperature was 28 ° С, relative air humidity 64%, wind speed 2 m / s. Method of application - continuous ground spraying of vegetative plants in the tillering stage. The equipment used is the Agrotop knapsack pneumatic sprayer, equipped with a two-meter rod. Working fluid consumption: 300 l / ha.

Accounting dates: June 26 - 30 days after spraying, July 11 - 45 days after spraying, July 18 - when cleaning. The method of accounting is carried out by the quantitative and quantitative-weight method at 4 survey sites of 0.25 m ² on each plot of experience. Method of harvesting and accounting of the crop yield: manually, using the test sheaf method with an accounting area of 1 m ² on each experimental plot. The harvest date is July 18, 2013. In order to obtain information on the possible toxicity of the emulsion of the biologically active synergistic composition for winter wheat plants, systematic visual observations were made of the treated plots. Under the effect of doses of the drug "Aleox" - 0.03 and 0.06 l / ha of visible inhibition of vegetative plants of winter wheat variety Moscow 39 was not observed. The plants did not differ in leaf color, height from plants in the control and reference variants, except for the dose of 0.06 l / ha, when the advanced development of the plants was observed.

The field small-scale field tests showed that high efficiency of application of the application rates of the studied drug was revealed. 30 days after the treatment of winter wheat crops in the phase of going out of the tube with doses of the preparation of 0.03 and 0.06 l / ha, the biologically active synergistic composition “Aleox” increased plant growth by 6 and 8%. When counted 45 days after spraying, the acceleration of plant growth was 8 and 10%, and their biomass was 7 and 9%, respectively.

Analysis of the elements of the structure of the harvest showed that the formation of the yield of winter wheat was mainly influenced by such an element of the structure as the mass of 1000 grains. On the variants with the use of all the norms of consumption of the drug, the mass of 1000 grains increased by 3.2-3.7 g compared to the untreated variant (the mass of 1000 grains in the control is 44.4 g).

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The structure of the harvest of winter wheat varieties Moscow 39 when using the drug "Aleks" is given in table. ten.

Table 10

Experience options Repetitions Structure elements The number of productive stems, pieces / m ² Plant height, cm Spike length cm The number of grains in spike PC. 1000 grains weight, g "Aleox" - 0.03 l / ha one 386 92.6 10.5 35.5 48.3 2 420 96.0 9.3 35.2 47.9 3 391 90.5 9.1 31.7 48.7 four 440 95.0 8.5 32.2 47.4 The average 409 93.0 9.3 33.6 48.1 "Aleox" - 0.06 l / ha one 448 93.9 9.1 34.5 46.6 2 472 90.1 8.6 36.0 47.8 3 412 88.5 9.0 35.6 48.4 four 388 89.6 8.5 31.7 47.6 The average 430 90.5 8.8 34.4 47.6 "Agrostimul" - 0.05 l / ha (standard) one 440 83.3 8.4 34.2 47.5 2 454 90.0 9.8 33.0 46.8 3 402 93.0 10.0 31.0 46.2 four 464 91.0 9.4 34.7 47.5 The average 440 89.3 9.4 33.2 47.0 Control one 430 89.0 8.5 32.0 43.6 2 414 90.0 9.0 30.8 44.4 3 390 91.0 8.6 31.7 44.8 four 422 92.0 8.8 31.0 45.0 The average 414 90.5 8.7 31.4 44.4

Spraying of crops in the phase of going into a cultured tube with the drug "Aleox" at doses of 0.03 and 0.06 l / ha contributed to an increase in the yield of winter wheat by 16.2 and 19.5% compared to the yield at the untreated control (the yield at the control - 36.4 c / ha).

The yield of winter wheat varieties Moscow 39 when using the drug "Aleox" is given in table. 10.1.

Table 10.1

Experience options Harvest by repetition, t / ha Average yield one 2 3 four centners per hectare % to control "Aleox" - 0.03 l / ha 42.0 41,8 43.0 42.4 42.3 116,0 "Aleox" - 0.06 l / ha 43.0 42.9 44.4 44.2 43.6 119.7 Agrostimul - 0.05 l / ha 41.6 41.5 42.7 41.7 41.9 114.9 Control 37.5 36.3 36.2 35.8 36.5 100.0

The small-scale registration tests of the drug "Aleox" under conditions of the Ryazan region on winter wheat showed that spraying of crops in the phase of going out of the tube did not have a negative impact on the further growth and development of the culture. The use of the drug "Aleox" in the studied doses significantly increased the resistance of cereal plants to powdery mildew, septoria, gelmintosporiozu. Biological efficacy of the drug "Aleox" against septorioz leaves in the experiments was 61%, brown rust - 83.3%. Biologically active synergistic composition "Aleox" at a dose of 0.03 l / ha showed high efficiency and resistance of crops to weather anomalies and increased yields.

Example 3

Under the conditions of obtaining the aqueous emulsion of the biologically active synergistic composition “Aleox” described in Example 1, field small-plot tests of the aqueous emulsion of the biologically active synergistic composition “Aleox” were carried out in a film greenhouse greenhouse. The greenhouse without emergency heating is equipped with a hose irrigation system. Soil soil - turf. The soil in the greenhouse before planting the plants was spilled, the required amount of Azofosk mineral fertilizers was introduced, bringing the level of supply of mineral nutrients to Ν ₂ -90, P ₂ O ₅ - 20, K ₂ O - 25, MD ₇₀ Ca ₉₀ . Seedlings were grown in a film greenhouse with emergency heating. Sowing of seeds was carried out on April 15 in boxes filled with soil mixture (1 part of field soil and 2 parts of sawdust). Before sowing, the soil mixture was spilled with copper sulfate to protect seedlings from root and neck rot. On the 8th day after the sprouting, the seedlings swooped down in a pots of 10/10 cm, which were placed in a film greenhouse with emergency heating. Caring for seedlings included 2 supplements of Kemira complex mineral fertilizer, watering, weeding, placement. Planting seedlings in a ground film greenhouse was carried out on May 31. The holes were dug in a two-line pattern of 90x40 with a distance of 35-40 cm between the holes. Planting density was 3.5 plants per 1 m. Plants were formed into 1 stem, tied to a trellis 2 m high by a string. The care of plants was carried out in accordance with the generally accepted

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by technology. Processing plants during the growing season was carried out on June 10, 20, July 1.

Spraying tests were carried out on vegetative tomato crops in closed ground. The area of experimental plots - 10 m ² . The area of the control plots - 5 m ² . The repetition of experiments fourfold.

The scheme of experience.

1. Control - without treatment.

2. The preparation "Aleox" - the first spraying of plants in the phase of the beginning of flowering of the first brush, the second spraying 10 days after the first, the third spraying 10 days after the second. The consumption of the drug is 60 ml / ha, the consumption of the working solution is 400 l / ha.

3. The drug "Agrostimul" - the first spraying of plants in the phase of the beginning of flowering of the first brush, the second spraying 10 days after the first, the third spraying 10 days after the second. Consumption of the drug is 60 ml / ha, consumption of the working solution is 400 l / ha.

During the tests, the acceleration of the growth of seedling biomass by 25-30% was noted, the onset of the onset of the flowering phase 2-3 days earlier, as well as the acceleration of fruit ripening 4-6 days compared with the control. At the same time, there was a decrease in plant damage by late blight, Alternaria, Septoria, and black bacterial spotting by 65-70% compared with the control.

Product quality was assessed by standard methods: the dry matter content was performed by the thermostat-weight method, the sugar content by the Bertrand method, and the vitamin C content by the IKMurry method. When processing the data, methods of variation statistics were used (BA Dospekhov, 1985).

As the records showed, the treatments with growth regulators had a positive effect on the timing of the passage of the main phenophases. When the drug was treated with the drug "Aleox", the differences with the control for the phase of the beginning of the formation and the beginning of ripening of the fruits were 4 days. Differences with the Agrostimul standard did not exceed 1 day.

The effect of the drug "Aleox" on the passage of phenophases of tomato plants is illustrated in Table. eleven.

T table 11

Experience option Massive bloom Start formations fruits Start ripening fruits 1. Control, without treatments 18.ΥΙ 28.U1 22. VII 2. "Agrostimul" standard, 60 ml / ha 17. VI 25.U1 19. VII 3. "Aleox", 60 ml / ha 17. VI 24ΑΙ 18. VII

Treatments of tomato plants with the drug "Aleox" had a positive effect on the acceleration of fruit ripening and the yield of early products. Significant differences were noted with the yield control for the first weeks of harvest. The increase in the total yield of the fruit under the influence of treatments with the preparation "Aleox" amounted to 7.1% of the control.

The effect of the drug "Aleox" on the yield of tomato is illustrated in Table. 12.

Table 12

Option Productivity, kg / m ² Control gain For the first 2 weeks collection Overall kg / m ² % 1 .Control 0.8 2.8 - - 2. "Aleox" dose 60 ml / ha 1.1 3.1 0.3 10.7 3. "Agrostimul" standard, dose 60 ml / ha 1.2 3.0 0.2 7.1 NDS 05 0.2 0.3 - -

The study of the biochemical composition of tomato fruits, depending on the variants of the experiment, showed that under the influence of treatments, the percentage of dry matter, sugars and vitamin C increased in comparison with the control.

The effect of the drug "Aleox" on the biochemical composition of the fruits of tomato are presented in Table. 13.

Table 13

Option of experience Dry substance, Sugar,% Vitamin C, mg /% Ν0 ₃ mg / kg Mono- Di- 1 .Control 7.9 3.5 0.08 17.5 23 2. "Agrostimul", standard, dose 60 9.5 3.91 0.60 25.0 38 3. "Aleox" dose 60 ml / ha 9.5 4.0 0.27 20.5 29

The content of nitrate nitrogen in products in all variants of the experiment was significantly less than the MPC value, which is 300 mg / kg wet weight for greenhouse tomatoes. High taste qualities of products are noted.

Thus, as a result of the experiments carried out, the positive effect of the drug "Aleox" on the yield of early production, the biochemical composition and taste of the fruits of tomato was established.

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Example 4

Under the conditions of obtaining the aqueous emulsion of the biologically active synergistic composition described in Example 1, in the season of 2013, small-field field trials of the drug Aleox were carried out to establish the biological effectiveness of the drug on a greenhouse cucumber.

The work was carried out in a film greenhouse with emergency heating, equipped with a hose irrigation system. Soil soil - turf. The soil in the greenhouse before planting the plants was spilled, and the required amount of Azofosk mineral fertilizers was applied. Seedlings were grown in 10x10 cm pots filled with a seedling mixture (lowland peat, Agrobalt riding peat, sawdust). Sowing seeds was carried out on May 6. Caring for seedlings included 2 supplements of Kemira complex mineral fertilizer, watering, weeding, placement. Planting seedlings in a ground film greenhouse was carried out on May 31. The holes were dug in a two-line pattern of 70x40 with a distance of 35-40 cm between the holes. Planting density was 4 plants per 1 m. The plants were formed into 1 stem, tied to a trellis 2 m high by a string. The care of the culture was carried out in accordance with the adopted cultivation technology. Plant treatments with the preparations "Aleox" and "Agrostimul" during the growing season were carried out on May 20 (phase 2-4 leaves), June 6 (the beginning of flowering), June 10-11 (mass flowering). The experiments were laid using the methods developed by the RAAS, the Moscow Agricultural Academy - the methods of an experimental business in vegetable and melon-growing (Belik, 1992) according to the following scheme.

1. Control. Without PPP processing.

2. "Agrostimul". Soaking the seeds before sowing for 2 hours, the drug consumption 0.1 ml / kg, the flow rate of the working solution 1 l / kg; spraying of plants: 1st - in the phase of 2-3 leaves, 2nd - in the phase of the beginning of flowering, 3rd - in the phase of mass flowering, drug consumption - 100 ml / ha, consumption of the working solution - 400 l / ha.

3. "Aleox". Soaking the seeds before sowing for 2 hours, the drug consumption 0.1 ml / kg, the flow rate of the working solution 1 l / kg; spraying of plants: 1st - in the phase of 2-3 leaves, 2nd - in the phase of the beginning of flowering, 3rd - in the phase of mass flowering, drug consumption - 100 ml / ha, consumption of the working solution - 400 l / ha.

4. "Aleox". Soaking the seeds before sowing for 2 hours, the drug consumption 0.1 ml / kg, the flow rate of the working solution 1 l / kg; spraying of plants: 1st - in the phase of 2-3 leaves, 2nd - in the phase of the beginning of flowering, 3rd - in the phase of mass flowering, the drug consumption is 200 ml / ha, the flow rate of the working solution is 400 l / ha.

The repetition of the experiment is 4 times. The area of the experimental plot was 10 m, and the register plot was 5 m. The weight of the crop was recorded by the gravimetric method. Product quality was assessed by standard methods: the dry matter content was performed by the thermostat-weight method, the sugar content by the Bertrand method, and the vitamin C content by the IK method. Murri.

When processing the data, methods of variation statistics were used (BA Dospekhov, 1985).

As the surveys showed, the treatment of cucumber plants with the preparation "Aleox" accelerated the time of mass flowering and the time of the beginning of the formation of fruits on day 3 compared to control, the time of mass ripening of fruits - by 3-4 days.

The effect of the drug "Aleox" on the timing of the passage of phenophases of cucumber are given in table. 14.

Table 14

Experience option Massive bloom Start formations Massive maturation 1. Control, without treatments 13. VI 21. VI ι.νιι 2. "Agrostimul", standard, seed soaking + triple spraying 11. VI 19. VI 28.U1 3. "Aleox", seed soaking + triple spraying plants 10. VI 18. VI 28.VI 4. "Aleox", seed soaking + triple spraying plants 10. VI 18. VI 27.U1

Due to the stimulation of the growth and development of cucumber plants, the treatment with the preparation "Aleox", increased the yield of early production by 0.8-1.1 kg / m ² and increased the total yield by 1.0-1.3 kg / m ² . The differences with the control are reliable and make up 21.1-28.9% in terms of yield for the first harvest and 10.814.1% in total yield. The reliably significant effect of the “Aleox” biological product was obtained at a consumption rate of 200 ml / ha.

Differences with the standard were insignificant and amounted to +0.2 kg / m. All variants of the experience showed a decrease in the output of non-standard products in the experience compared to the control (Table 2).

The effect of the drug "Aleox" on the yield of cucumber is illustrated in Table. 15.

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Table 15

Option of experience Productivity, kg / m ² Control gain Standard, % For the first 2 weeks Overall kg / m ² % 1. Control, without treatments 3.8 9.2 84.3 2. "Agrostimul", standard, seed soaking + three spraying of plants, 100 ml / ha 4.7 10.3 1.1 11.9 90.2 3. "Aleox", soaking seeds + spraying plants three times, 100 ml / ha 4.6 10.2 1.0 10.8 91.4 4. "Aleox", soaking seeds + spraying plants three times, 200 ml / ha 4.9 10.5 1,3 14.1 92.7 NDS ₀₅ 0.8 1.1 - - -

The study of the biochemical composition of cucumber fruits, depending on the variants of the experiment, showed that the percentage of dry matter and sugar in the fruits did not change significantly compared with the control when treated with Aleox. There was an increase in the content of vitamin C in fruits by more than 50% to control. The content of nitrate nitrogen in products in all variants of the experiment was significantly less than the MPC value, which is 400 mg / kg wet weight for greenhouse cucumbers. High taste qualities of products are noted.

The effect of growth regulator "Aleox" on the biochemical composition of cucumber is illustrated in Table.

sixteen.

Table 16

Experience option Dry substance,% Sahara, % Vitamin C, mg /% Ν0 ₃ mg / kg 1. Control, without treatments 5.0 2.28 4.0 165 2. "Agrostimul", standard, seed soaking + three spraying of plants, 100 ml / ha 4.9 2.52 4.4 197 3. "Aleox", soaking seeds + spraying plants three times, 100 ml / ha 4.5 2.15 6.5 200 4. "Aleox", soaking seeds + spraying plants three times, 200 ml / ha 5.1 2.16 7.1 205

Thus, as a result of the experiments, a positive effect of the drug "Aleox" on the processes of growth and development of cucumber was established. It was revealed that processing at the beginning of the flowering phase has a significant impact on the yield of early production and on the total yield of cucumber fruits. Given the effectiveness of the drug "Aleox", the performers consider it possible to recommend it for use on cucumber culture in protected ground. The application rules are as follows: soaking the seeds before sowing for 2 hours, the consumption of the preparation - 0.1 ml / kg, the consumption of the working solution - 1 l / kg; spraying of plants: 1st - in the phase of 2-3 leaves, 2nd - in the phase of the beginning of flowering, 3rd - in the phase of mass flowering. The consumption of the drug is 200 ml / ha, the consumption of the working solution is 400 l / ha.

Example 5

Under the conditions of obtaining the aqueous emulsion of the biologically active synergistic composition described in Example 1, in the season of 2013, small-field field trials of the drug Aleox were conducted to establish the biological effectiveness of the drug on potatoes. The work was performed in the field according to the scheme of experience.

1. Control. Without processing.

2. "Agrostimul". Pre-treatment of tubers, drug consumption 20 ml / t, consumption of working solution 10 l / t; spraying of plants: 1st - in the period of mass flowering, 2nd - 20 days after the first spraying, the consumption of the preparation is 100 ml / ha, the consumption of the working solution is 300 l / ha.

3. "Aleox". Pre-treatment of tubers, drug consumption 20 ml / t, consumption of working solution 10 l / t; spraying of plants: 1st - in the phase of budding-beginning of flowering, 2nd - 20 days after the first spraying, the drug consumption is 100 ml / ha, the working solution consumption is 300 l / ha.

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The area of experimental plots is 100 m ² , the area of accounting plots is 50 m ² . The repetition is fourfold. Pre-treatment of potato tubers and spraying of plants during the growing season was carried out using a knapsack sprayer.

The working solution of the preparations was prepared immediately before performing the treatments. To prepare the working solution, the required amount of plant growth regulator was measured per treatment. Next, the sprayer tank was filled with about half of the water, the required amount of the preparations diluted in a small volume of water was added and mixed. Then water was added to the calculated volume, the solution was thoroughly mixed again and processed.

Spraying of plants was carried out in the morning or evening in windless weather or at a wind speed of not more than 5-6 m / s and an air temperature of 18-22 ° C.

The soil of the experimental plot is a typical heavy loamy chernozem with a humus content of 4.5–5.0%, an amount of absorbed bases of 37–40 mg eq./100 g, a hydrolytic acidity of the soil 1.6–1.8 mg eq. / 100 g of soil, pH salt extract of 5.8-5.9. The content of mobile phosphorus and exchangeable potassium (according to Chirikov), respectively, is 55-60 and 105-125 mg / kg of soil.

Previous crop - winter wheat. In September 2012, the site was plowed to a depth of 2527 cm. In April 2013, the land was harrowed at the experimental site. Planting of potatoes with previous soil preparation was carried out on May 12. The density of the standing of potato plants was determined in the phase of full germination and before harvesting. Before harvesting the potatoes, tubers were taken (10 bushes from each plot) to determine the crop structure.

Accounting for the yield of tubers was carried out when harvesting from each plot separately by a continuous method. The yield data was processed by the method of variance analysis (according to BA Dospekhov, 1985).

From planting to harvest, the passage of the main phases of development was observed: seedlings, budding, flowering, harvesting.

During the growing season, we recorded the weed infestation, the appearance of major diseases and pests. Crops were treated with herbicides, insecticides, fungicides. Counting the density of the standing of potato plants in the phase of full germination and before harvesting the culture did not reveal differences in this indicator between the control and experimental variants. The structure of the potato harvest is given in table. 17

Table 17

Options of experience The number of tubers from 1 plant Total small fraction seed fraction food fraction qty PC. mass, g qty PC. mass, g qty PC. mass, g qty PC. mass, g Control 7.3 409.1 1.8 39.6 3.4 180,5 2D 189.0 "Agrostimul" 8.1 472.0 1.7 37.4 3.8 200.6 2.6 234.0 "Aleox" 8.5 514.0 1.6 35.2 3.9 208.8 3.0 270.0 NSR Lake 0.5 24.8 0.2 2.2 0.3 10.2 0.2 12.3

The data presented in table. 20 show how the use of the drug "Aleox" affects the formation of the structural elements of the potato harvest.

The yield and quality of potato tubers are given in table. 18.

Table 18

Experience option Productivity t / ha Gain to control, t / ha Starch, % The content of nitrates in tubers, mg / kg Control 20.7 - 12.15 8.35 "Agrostimul" 24.1 3.4 12.93 7.52 "Aleox" 26.4 5.7 14.73 5.91 NDS ₀₅ 1.6 0.68 0.34

The yield of potatoes in the experimental variants increased in comparison with the control by 3.4-5.7 t / ha. The maximum increase was obtained in the experimental variant with the drug "Aleox". Pre-treatment of tubers, drug consumption 20 ml / t, consumption of working solution 10 l / t; spraying of plants: 1st - in the phase of budding-beginning of flowering, 2nd - 20 days after the first spraying, the drug consumption is 200 ml / ha, the working solution consumption is 300 l / ha.

Based on the data obtained, it can be concluded that the use of the new biologically active synergistic composition "Aleox" on crops of potatoes contributes to an increase in yield compared with the control variant.

The obtained test results of the drug allow us to recommend it for practical use.

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Example 6

Under the conditions of obtaining the aqueous emulsion of the biologically active synergistic composition "Aleox" described in Example 1, tests were carried out to establish the biological activity of the preparation "Aleox" and the optimal concentrations of its solution when treating pea seeds before sowing.

The studies were carried out in a laboratory experiment (laboratory screening) - in Petri dishes. Bed for seeds - filter paper. 50 or 100 seeds were placed in each cup (control and experienced variants). The germination energy and germination rate was determined in the time specified for each crop in the state standard - GOST 12038-84 (Crop seeds. Methods for determining germination). Seeds were germinated in a thermostat at a temperature of 25 ° C.

The experiment was conducted in 9 variants in 4-fold repetition. All observations, measurements, calculations and notes were recorded in a laboratory journal, to which are also attached photographs of the seedlings obtained. In tab. 19 presents the results of the effect of the drug on the germination of pea seeds.

Table 19

Experience option Germination, % Deviation from the office. % one . Water 33 2. The drug 500 ml / 10 l of water ("Aleox" 0.5 l / t) 31 3. The drug 250 ml / 10 l of water ("Aleox" 0.25 l / t) 51 + 18 4. Preparation of 100 ml / 10 l of water ("Aleox" 0.1 l / t) 9 5, the Drug 80 ml / 10 l of water ("Aleox" 0.08 l / t) 37 +4 b. The drug 40 ml / 10 l of water ("Aleox" 0.04 l / t) 36 +3 7. Preparation of 20 ml / 10 l of water ("Aleox" 0.02 l / t) 49 +16 8. Drug 10 ml / 10 l of water ("Aleox" 0.01 l / t) 25 9. Preparation 5 ml / 10 l of water ("Aleox" 0.005 l / t) 23

The best results compared with the control (33%) were observed in variants 3, 7 and 5 (51, 49 and 37%, respectively).

The test results of the drug "Aleox" on pea seeds are given in Table. 20.

Table 20

Option Drug rate Germination, % Mass, g The length of the spine, cm Sprout length, cm one Water 33 7.28 4.15 2.57 2 Drug 500 ml / 10 l of water ("Aleox" 0.5 l / t) 31 7.32 4.17 3.29 3 The drug 250 ml / 10 l of water ("Aleox" 0.25 l / t) 51 7.18 3.42 3.24 four The drug 100 ml / 10 l of water ("Aleox" 0.1 l / t) 9 7.04 4.04 2.29 five Drug 80 ml / 10 l of water ("Aleox" 0.08 l / t) 37 7.24 3.05 2.18 6 The drug 40 ml / 10 l of water ("Aleox" 0.04 l / t) 36 7.25 4.69 3.18 7 The drug 20 ml / 10 l of water ("Aleox" 0.02 l / t) 49 7.23 3.81 2.93 eight The drug 10 ml / 10 l of water ("Aleox" 0.01 l / t) 25 6.52 2.94 1.84 9 The drug 5 ml / 10 l of water ("Aleox" 0,005 l / t) 23 7.19 3.84 3.07

In terms of the mass of seedlings, the best variant compared with the control was the variant with the drug consumption rate of 500 ml / 10 l of water - 7.32 g (control - 7.28 g). The remaining options were inferior to the control. Along the length of the roots, variants 2 and 6 stood out (norms of 500 ml / 10 l of water and 40 ml / 10 l of water). Along the length of the germ, the following variants are noted: 2 (500 ml / 10 l of water); 3 (250 ml / 10 l of water); 6 (40 ml / 10 l of water); 7 (20 ml / 10 l of water) and 9 (5 ml / 10 l of water). Thus, the best in most indicators were options 2 (500 ml / 10 l of water) and 6 (40 ml / 10 l of water). But since the rate of 500 ml / 10 l of water is large enough for a growth regulator, it is better to recommend a rate of 40 ml / 10 l of water.

For all pea seeds, according to all indicators taken into account (mass of seedlings, germination, length of sprouts and roots, absence of mold on seeds), two variants were noted with the preparation consumption rates

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500 ml / 10 l of water and 40 ml / 10 l of water, from which the variant 40 ml / 10 l of water was isolated (germination rate 36%, mass of seedlings 7.25 g, root length - 4.69 cm, sprout length - 3, 18 cm; control - 33%, 7.28 g, 4.15 cm and 2.57 cm, respectively). Moreover, the treatment of pea seeds with the drug "Aleox" in the optimal concentration (40 ml / 10 l of water) has a greater effect on the formation of the sprout than the root. So, in relation to the control variant, the length of the sprout increased by 24%, the length of the spine by 13%, and the germination rate increased by 9%.

Screening studies to establish the biological activity of the drug "Aleox" in the treatment of seeds before sowing showed a fairly high efficiency. On the basis of the results obtained, the consumption rate of the biologically active synergistic composition "Aleox" on peas is the most effective - 40 ml per 10 l of water per 1 ton of seeds.

Thus, during a comprehensive study, it was revealed that a new biologically active synergistic composition has a positive effect on plants: increases yield and frost resistance, accelerates maturation, the onset of biological and technological maturity, accelerates seed germination, increases their germination and initial growth activity, reduces fungal and bacterial morbidity of plants, accelerates the growth of the root system and increases its mass.

Biologically active synergistic composition "Aleox" is promising in its application to wheat, barley, rye, buckwheat, soybean, rapeseed, sugar beet, pea, sunflower, potato, flax, grapes, tomato and cucumber crops.

Information sources.

1. RF patent №2463759, А01С 1/06, А01С 1/08, 2011.

2. All-Russian Scientific Research Institute of Medicinal and Aromatic Plants (VILFR). Kolkhir Vladimir Karlovich. Search and development of phytopreparations containing triterpenoids and flavonoids. The thesis in the form of a report for the degree of Doctor of Medical Sciences. Kupavna, 2002. Ir: //tebya1-b188.sots/bosgeabeg/55384/a? #? Rade = T

3. RF patent number 2292896, А61К 36/00, А61К 31/717, А61Р 39/06, 2005.

4. Experience of preclinical research on the example of Olipifata / Ed. V.A. Filova, A.M. Berkovich. - SPb: NIKA, 2002. - 288 p.

5. The international application of joint-stock company 2005/058476, ВПП 13/16, 2005.

6. International application AO 2008/123797, АКК 9/10, АКК 9/107, АCК 31/352, АCК 31/015, АCК 36/324, АCК 33/00, А61К 8/06, А61К 9/10, А61К 47/10, AC 47/14, 2008.

7. Patent of the Russian Federation No. 2229213, А01О 1/00, /01Ν 65/00, 2002.

8. State catalog of pesticides and agrochemicals of the Russian Federation. Registration numbers 0164-06111-042-0-1-3-0 and 1986-10-111-042-0-1-3-1.

9. Soiksb Kedts1abop (EU) 34ο 834/2007 at £ 28 1st 2007, opted out to the top of the site for the first time in Europe and Europe, Kedi1abop (EEC) No. 2092/91. O £ us1a1 1oitpa1 about £ 1st eigoreup Itop, L 189/1,

20.7.2007.

10. Sotbbop Kedi1abop (EU) No. 889/2008 about £ 5 8er1t 2008 1 Ushd bo \ n pbShebbie1 £ 8 from 1ее 1tr1tep1abop о £ Soipps Kedi1abop (EU) No. 834/2007 op o rtbysbop upb 1šefezePnIeop (ies) 899/2007 Gedagb Ugogda Rtobisbop, Laurenster Sopb1. About £ іс1а1 1оитпа1 о £ 1Пе Egoreup shop. L 250/1,

18.9.2008.

11. Patent application of the Republic of Lithuania No. 2014070, 2014.05.21.

12. State catalog of pesticides and agrochemicals of the Russian Federation. Registration number 002-07-4721.

Claims (3)

CLAIM 1. Fertilizer and plant protection agent in the form of an aqueous emulsion, which includes a complex of bioflavonoids with lignin in a synergistic ratio by weight of components 1.0: 0.19-0.27, an organic solvent and a natural emulsifier, with an arabinogalactan emulsion in the aqueous phase 12 -18 g / l, dihydroquercetin 0.23-0.28 wt.% And water-soluble triterpenoids in a weight ratio of 0.011-0.09 wt.%. 2. Fertilizer and plant protection product according to claim 1, where lecithin in the form of an oil solution is used as a natural emulsifier, taken in an amount of 6-9% by volume of the emulsion. 3. The use of an aqueous emulsion in PP. 1 and 2 by preparing a working solution by diluting the emulsion in water, followed by applying it on the surface of seeds of crops or spraying vegetative cultivated plants on the surface.