KR101206707B1 - Cultivation method of plant with increased contents of functional materials - Google Patents

Cultivation method of plant with increased contents of functional materials Download PDF

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KR101206707B1
KR101206707B1 KR1020100034981A KR20100034981A KR101206707B1 KR 101206707 B1 KR101206707 B1 KR 101206707B1 KR 1020100034981 A KR1020100034981 A KR 1020100034981A KR 20100034981 A KR20100034981 A KR 20100034981A KR 101206707 B1 KR101206707 B1 KR 101206707B1
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content
buckwheat
plant
chloride
treatment
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KR20110115468A (en
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임정호
김현진
정진웅
박기재
김범근
정승원
김명호
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한국식품연구원
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00—Cultivation of specific crops or plants not otherwise provided for
    • A01G22/20—Cereals
    • A01G22/22—Rice
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01C—PLANTING; SOWING; FERTILISING
    • A01C21/00—Methods of fertilising, sowing or planting
    • A01C21/005—Following a specific plan, e.g. pattern
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G5/00—Fertilisers characterised by their form
    • C05G5/20—Liquid fertilisers
    • C05G5/23—Solutions
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S47/00—Plant husbandry
    • Y10S47/06—Plant growth regulation by control of light thereon

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Soil Sciences (AREA)
  • Pest Control & Pesticides (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Botany (AREA)
  • Cultivation Of Plants (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

본 발명은 식물체의 재배방법에 있어서, 염화물, 메틸자스모네이트, 또는 염화물과 메틸자스모네이트를 식물체에 처리하여 기능성 물질의 함량을 증가사키는 단계를 포함하는 것을 특징으로 하는 기능성 물질의 함량이 증가된 식물체의 재배방법을 제공한다.The present invention provides a method of growing a plant, comprising the steps of increasing the content of the functional material by treating the chloride, methyl jasmonate, or chloride and methyl jasmonate to the plant content of the functional material, characterized in that Provides a method for growing plants.

Description

기능성 물질의 함량이 증진된 식물체의 재배방법{CULTIVATION METHOD OF PLANT WITH INCREASED CONTENTS OF FUNCTIONAL MATERIALS}Cultivation method of plant with enhanced content of functional substance {CULTIVATION METHOD OF PLANT WITH INCREASED CONTENTS OF FUNCTIONAL MATERIALS}

본 발명은 기능성 물질의 함량이 증진된 식물체의 재배방법에 관한 것으로, 보다 상세하게는 식물체에 스트레스를 적절하게 유발하여 특히 자기방어능력에 의해 새싹 및 어린잎 채소에 함유된 기능성 물질의 함량을 대량으로 증진시킬 수 있는 식물체의 재배방법에 관한 것이다.The present invention relates to a cultivation method of a plant having an increased content of a functional substance, and more particularly, to induce stress in the plant appropriately, in particular, a large amount of the functional substance contained in sprouts and young leafy vegetables by self-defense ability. It relates to a cultivation method of plants that can be promoted.

식물체에 함유된 다양한 폴리페놀 화합물은 호흡기 감염증 병원체인 백일해균과 인플루엔자균에 대한 감염 저지능력이 있으며, 치아의 무기질인 칼슘염을 보호하고 식품으로 인체에 들어오는 산(酸)에 의한 용출을 막아 치아의 부식작용을 방지하며, 또한 혈중 콜레스테롤의 상승억제작용을 발휘하여 동맥경화 및 심근경색, 뇌졸중 등을 예방하고 본태성고혈압을 억제하는 기능을 가지고 있는 것으로 알려져 있다. Various polyphenolic compounds contained in plants have the ability to prevent infections of pertussis and influenza bacteria, which are pathogens of respiratory infections. It is known to have a function of preventing the corrosive action of the blood and also inhibiting the increase of blood cholesterol, preventing arteriosclerosis, myocardial infarction and stroke and suppressing essential hypertension.

또한 식품 중의 지방질은 일광 등의 산화 촉진작용에 의하여 공기 중의 산소를 취하면서 신속하게 과산화지질을 생성하는데, 이것은 특히 노화와 암발생의 관계가 깊은 것으로 알려져 있는데 지방질의 산화를 방지하는, 즉 항산화작용을 하는 것이 바로 폴리페놀로 알려져 있다.In addition, fats in foods rapidly produce lipid peroxide by taking oxygen in the air by oxidation-promoting action such as sunlight, which is known to have a particularly strong relationship between aging and cancer. It is known as polyphenol.

최근 식탁에서의 웰빙붐이 거세고 일고 있는 가운데, 새싹 또는 어린 채소잎을 이용한 각종 식품들이 인기를 구가하고 있다. 하지만, 통상적으로 이러한 새싹 또는 어린 잎에 존재하는 폴리페놀의 함량은 제한적인 상황이다. 특히 메밀에 함유된 것으로 알려진 폴리페놀성분 중 루틴(rutin)이라는 화합물은 모세혈관을 강화시켜 뇌출혈을 예방하는 효과가 큰 것으로 알려져 이를 이용한 다양한 식품개발이 시도되고 있으나 여전히 그 함량이 크지 않아 다량으로 섭취해야 하는 한계가 있다. As the well-being boom on the table is rising and rising recently, various foods using sprouts or young vegetable leaves are becoming popular. Typically, however, the content of polyphenols present in such shoots or young leaves is limited. In particular, among the polyphenols known to be contained in buckwheat, a compound called rutin is known to have a great effect of preventing cerebral hemorrhage by strengthening capillaries, but various food developments have been tried. There is a limit to this.

본 발명은 상기한 바와 같이 종래기술이 가지는 문제를 해결하기 위해 제안된 것으로, 그 목적은 식물체에 스트레스를 적절하게 유발하여 특히 자기방어능력에 의해 새싹 및 어린잎 채소에 함유된 기능성 물질의 함량을 대량으로 증진시킬 수 있는 식물체의 재배방법을 제공함에 있다. The present invention has been proposed to solve the problems of the prior art as described above, its purpose is to induce stress appropriately to the plant, in particular by the self-defense ability to contain the content of functional substances contained in sprouts and young leafy vegetables The present invention provides a method for growing plants that can be promoted in large quantities.

상기한 바와 같은 본 발명의 기술적 과제는 다음과 같은 수단에 의해 달성되어진다.The technical problem of the present invention as described above is achieved by the following means.

(1) 식물체의 재배방법에 있어서, 염화물, 메틸자스모네이트, 또는 염화물과 메틸자스모네이트를 식물체에 처리하여 기능성 물질의 함량을 증가시키는 단계를 포함하는 것을 특징으로 하는 기능성 물질의 함량이 증가된 식물체의 재배방법.
(1) A method of cultivating a plant, wherein the content of the functional substance is increased by treating chloride, methyljasmonate, or chloride and methyljamonate with the plant to increase the content of the functional substance. Method of growing plants.

(2) 제 1항에 있어서,(2) The method according to claim 1,

식물체는 메밀인 것을 특징으로 하는 기능성 물질의 함량이 증가된 식물체의 재배방법.
The plant is a method of cultivating a plant having an increased content of a functional substance, characterized in that the buckwheat.

(3) 제 1항에 있어서,(3) The method according to claim 1,

기능성 물질은 루틴인 것을 특징으로 하는 기능성 물질의 함량이 증가된 식물체의 재배방법.
Functional material is a cultivation method of a plant having an increased content of the functional material, characterized in that the routine.

(4) 제 1항에 있어서,(4) The method according to 1,

염화물은 염화나트륨인 것을 특징으로 하는 기능성 물질의 함량이 증가된 식물체의 재배방법.
A chloride is a method for cultivating a plant having an increased content of a functional substance, characterized in that sodium chloride.

(5) 제 1항에 있어서,(5) The method according to claim 1,

염화물은 재배수에 농도 10-200mM로 조절하여 식물체에 처리되는 것을 특징으로 하는 기능성 물질의 함량이 증가된 식물체의 재배방법.
The method of cultivating plants with increased content of functional substances, characterized in that the chloride is treated to plants by adjusting the concentration to 10-200mM in the cultivation water.

(6) 제 1항에 있어서,(6) The method according to 1,

재배수내 염화물과 메틸자스모네이트의 농도비는 몰농도로 10:1-1:10인 것을 특징으로 하는 기능성 물질의 함량이 증가된 식물체의 재배방법.
The concentration ratio of chloride and methyl jasmonate in the cultivation water is a molar concentration of 10: 1-1: 10.

(7) 제 1항에 있어서,(7) The method according to 1,

재배수내 염화물과 메틸자스모네이트의 농도비는 몰농도로 100:1-1:100인 것을 특징으로 하는 기능성 물질의 함량이 증가된 식물체의 재배방법.
Concentration ratio of chloride and methyl jasmonate in the cultivation water is a method of cultivating plants with increased content of functional substances, characterized in that the molar concentration of 100: 1-1: 100.

(8) 제 1항에 있어서,(8) The method according to 1,

식물체에 빛을 처리해 주는 단계를 더 포함하는 것을 특징으로 하는 기능성 물질의 함량이 증가된 식물체의 재배방법.
Method of cultivating the plant with increased content of the functional material, characterized in that it further comprises the step of treating light to the plant.

(9) 제 1항 내지 제 8항에서 선택된 어느 한 항에 의해 재배된 기능성 물질의 함량이 증가된 식물체.
(9) A plant having an increased content of the functional substance cultivated by any one of the above-mentioned items.

(10) 제 9항에 있어서,(10) The method according to 9,

식물체는 메밀 유식물체인 것을 특징으로 하는 기능성 물질의 함량이 증가된 식물체.
The plant is a plant with an increased content of a functional substance, characterized in that the buckwheat seedlings.

본 발명에 의하면 식물체에 스트레스를 적절하게 유발하여 특히 자기방어능력에 의해 새싹 및 어린잎 채소에 함유된 기능성 물질의 함량을 대량으로 증진시킬 수 있다.
According to the present invention, it is possible to appropriately induce stress on the plant, and in particular, to increase the content of functional substances contained in the shoots and young leafy vegetables by the self-defense ability.

도 1은 메틸자스모네이트를 메밀 새싹에 7일간 처리한 처리구(MeJA)와 무처리구(control)의 페놀화합물의 크로마토그램(A), 총 페놀화합물의 함량(B), 주 화합물의 구조(C), 및 각 화합물에 대한 영향(D)을 나타낸 것이다.
도 2는 메틸자스모네이트를 메밀 새싹에 7일간 처리한 처리구(MeJA)와 무처리구(control)의 항산화 활성을 측정한 결과이다.
도 3은 염화나트륨의 농도별 처리에 따른 메밀새싹의 생중량의 변화량을 측정한 결과이다(각 데이터는 40개 새싹을 대상으로 3회 반복측정하여 평균±표준오차로 표시).
도 4a는 염화나트륨의 농도별 처리에 따른 메밀새싹의 루틴함량의 변화량을 측정한 결과이다(각 데이터는 40개 새싹을 대상으로 3회 반복측정하여 평균±표준오차로 표시).
도 4b는 염화나트륨의 농도별 처리에 따른 메밀새싹의 총 페놀함량의 변화량을 측정한 결과이다(각 데이터는 40개 새싹을 대상으로 3회 반복측정하여 평균±표준오차로 표시).
도 5는 염화나트륨의 농도별 처리에 따른 메밀새싹의 항산화활성의 변화량을 측정한 결과이다(각 데이터는 40개 새싹을 대상으로 3회 반복측정하여 평균±표준오차로 표시).
도 6은 염화나트륨과 메틸자스모네이트의 혼합처리에 따른 메밀새싹의 생중량의 변화량을 측정한 결과이다(각 데이터는 40개 새싹을 대상으로 3회 반복측정하여 평균±표준오차로 표시).
도 7a는 염화나트륨과 메틸자스모네이트의 혼합처리에 따른 메밀새싹의 루틴함량의 변화량을 측정한 결과이다(각 데이터는 40개 새싹을 대상으로 3회 반복측정하여 평균±표준오차로 표시).
도 7b는 염화나트륨과 메틸자스모네이트의 혼합처리에 따른 메밀새싹의 총 페놀함량의 변화량을 측정한 결과이다(각 데이터는 40개 새싹을 대상으로 3회 반복측정하여 평균±표준오차로 표시).
도 8은 염화나트륨과 메틸자스모네이트의 혼합처리에 따른 메밀새싹의 항산화활성의 변화량을 측정한 결과이다(각 데이터는 40개 새싹을 대상으로 3회 반복측정하여 평균±표준오차로 표시).1 is a chromatogram (A), total phenolic compound content (B), and the structure of the main compound (C) of phenolic compounds treated with methyl jasmonate in buckwheat sprouts for 7 days (MeJA) and untreated (control). , And the influence (D) on each compound.
Figure 2 is a result of measuring the antioxidant activity of the treated group (MeJA) treated with methyl jasmonate in buckwheat sprout for 7 days (control).
Figure 3 is the result of measuring the change in the raw weight of the buckwheat sprout according to the treatment of the concentration of sodium chloride (each data is measured by repeating three times for 40 shoots expressed as mean ± standard error).
Figure 4a is the result of measuring the change in the rutin content of buckwheat sprout according to the treatment of sodium chloride concentration (each data is measured by repeating three times for 40 shoots expressed as mean ± standard error).
Figure 4b is the result of measuring the change in the total phenolic content of buckwheat sprouts according to the concentration of sodium chloride treatment (each data is repeated 40 times to measure the average ± standard error).
Figure 5 is the result of measuring the change in the antioxidant activity of the buckwheat sprout according to the treatment of the concentration of sodium chloride (each data is measured by repeating three times for 40 shoots expressed as mean ± standard error).
Figure 6 is the result of measuring the change in the raw weight of buckwheat sprouts according to the mixing treatment of sodium chloride and methyl jasmonate (each data is repeated three times for 40 shoots expressed as the average ± standard error).
Figure 7a is a result of measuring the change in the routine content of buckwheat sprouts according to the mixing treatment of sodium chloride and methyl jasmonate (each data is repeated three times for 40 shoots expressed as the average ± standard error).
Figure 7b is the result of measuring the change in the total phenolic content of buckwheat sprouts according to the mixing treatment of sodium chloride and methyl jasmonate (each data is repeated three times for 40 shoots expressed as mean ± standard error).
Figure 8 is the result of measuring the change in the antioxidant activity of buckwheat sprouts according to the mixing treatment of sodium chloride and methyl jasmonate (each data is repeated three times for 40 shoots expressed as mean ± standard error).

본 발명은 염화물, 메틸자스모네이트, 또는 염화물과 메틸자스모네이트를 식물체에 처리하여 기능성 물질의 함량을 증가시키는 단계를 포함하는 것을 특징으로 하는 기능성 물질의 함량이 증가된 식물체의 재배방법을 제공한다.
The present invention provides a method for cultivating a plant having an increased content of a functional substance, comprising the step of increasing the content of the functional substance by treating the plant with chloride, methyl jasmonate, or chloride and methyl jasmonate. do.

이하, 본 발명의 내용을 보다 상세하게 설명하면 다음과 같다.
Hereinafter, the content of the present invention in more detail as follows.

본 발명에 따른 식물체의 재배방법이 목적으로 하는 식물체는 바람직하게는 새싹 및 어린잎 채소로서, 염화물, 메틸자스모네이트, 또는 염화물과 메틸자스모네이트의 혼합처리에 따른 외부적 스트레스에 대하여 자기방어능력에 의해 기능성 물질을 대량으로 생산하는 것으로 한다. 대표적인 예로는 상기 식물체는 메밀이다.
Plants aimed at the method of cultivating plants according to the present invention are preferably sprouts and young leafy vegetables, which are self-defense against external stress caused by chloride, methyljasmonate, or a mixture of chloride and methyljasmonate. It is assumed that a functional substance is produced in large quantities by the ability. As a representative example, the plant is buckwheat.

상기 본 발명에서 「기능성 물질」은 루틴을 포함하는 폴리페놀 화합물 및 이들의 유도체를 포함하며, 예를 들어 루틴, 호모오리엔틴, 오리엔틴, 바이텍신 등을 포함하여 인체에 섭취가능하고 인체에 유용한 기능성을 발휘하는 어떠한 물질도 포함될 수 있지만, 바람직하게는 상기 루틴을 든다.In the present invention, the "functional substance" includes polyphenol compounds including rutin and derivatives thereof, and includes, for example, rutin, homoorientin, orientin, vitexin, and the like, which are ingestible and useful for the human body. Any material that exhibits functionality may be included, but preferably the routine is mentioned.

본 발명에서의 기능성 물질의 대량 생산방법은 식물체에 공급되는 재배수에 염화물, 또는 염화물과 메틸자스모네이트를 식물체에 처리하는 과정을 포함한다. 이때 본 발명에 사용되는 염화물의 종류는 염화나트륨, 염화칼륨, 염화칼슘 등을 들 수 있지만, 바람직하게는 염화나트륨이다.
The mass production method of the functional substance in the present invention includes a process of treating the plant with chloride, or chloride and methyl jasmonate in the cultivation water supplied to the plant. At this time, the chloride used in the present invention may be sodium chloride, potassium chloride, calcium chloride, etc., but is preferably sodium chloride.

염화물의 처리시 특히 한정되는 것은 아니나, 바람직하게는 재배수에 10 내지 200mM 가 되도록 조성된다. 만일 10mM 농도 이하로 첨가될 경우 염화물의 첨가에 따른 기능성 물질의 증대효과가 크지 않고, 200mM를 초과하여 처리할 경우 오히려 식물체의 생육저하를 초래하는 등의 문제가 야기된다.
The treatment of chloride is not particularly limited, but is preferably such that the cultivation water is 10 to 200 mM. If it is added below 10mM concentration, the effect of increasing the functional substance according to the addition of chloride is not large, and if the treatment exceeds 200mM, problems such as causing the growth of the plant rather causes.

메틸자스모네이트의 재배수에서의 첨가 농도는 바람직하게는 0.01 내지 200mM, 바람직하게는 0.1 내지 2 mM이 되도록 조성된다. 만일 0.01 mM 농도 이하로 첨가될 경우 메틸자스모네이트의 첨가에 따른 기능성 물질의 증대효과가 크지 않고, 200 mM를 초과하여 처리할 경우 오히려 식물체의 생육저하를 초래하는 등의 문제가 야기된다.
The concentration of the methyl jasmonate added in the cultivated water is preferably set to 0.01 to 200 mM, preferably 0.1 to 2 mM. If the concentration is less than 0.01 mM, the effect of increasing the functional substance according to the addition of methyl jasmonate is not great, and if the treatment exceeds 200 mM, problems such as the growth of plants are caused.

바람직하게는 상기 염화물과 메틸자스모네이트는 함께 처리되어지며 특히 한정되는 것은 아니나, 바람직하게는 그 조성비가 몰농도로 100:1-1:100, 바람직하게는 10:1-1:10인 것으로 한다. 상기 몰농도비로 조성되어질 경우 특히 기능성 물질의 생산량에 있어서 생육을 지장을 주지 않는 선에서 상승적인 효과를 기대할 수 있기 때문이다.
Preferably, the chloride and methyljasmonate are treated together and are not particularly limited, but preferably the composition ratio is 100: 1-1: 100, preferably 10: 1-1: 10 in molar concentration. do. This is because when the molar concentration ratio is formulated, a synergistic effect can be expected, especially in the production amount of the functional substance, without affecting the growth.

상기와 같은 조성을 갖는 재배수는 목적으로 하는 식물체의 종류에 따라 정해지는 처리시기 및 횟수에 따라 처리하는 것으로 충분하다. 예를 들어 메밀 새싹의 경우 1～3일에 1번 정도로 1회 100mL～4L/m2씩 공급하면 된다.
It is sufficient to treat the cultivation water having the composition as described above according to the treatment time and the number of times determined according to the kind of the target plant. For example, in the case of buckwheat sprout, supply 100mL ~ 4L / m 2 once per 1 ~ 3 days.

또한, 본 발명에서는 기능성 물질의 함량을 더욱 높이기 위하여 식물체에 빛을 처리할 수 있다. 빛의 처리는 500 Lux 이상으로 1일 4~12시간 정도 처리하는 것으로 충분하며, 이에 의하면 루틴을 포함한 페놀화합물의 함량이 크게 증가하는 것이 확인되었다.
In addition, in the present invention, it is possible to treat the light to the plant in order to further increase the content of the functional material. The treatment of light is more than 500 Lux for 4 to 12 hours per day, and it is confirmed that the content of phenolic compound including rutin increases significantly.

상기 본 발명에 따른 재배방법을 이용하여 메밀 새싹을 대상으로 1주일간 처리한 결과에서 메틸자스모네이트의 처리결과 루틴의 함량이 1.5배 이상 증가하고, 염화나트륨을 처리한 결과 1.8배 이상 증가하는 것을 확인하였다. 또 총 페놀화합물의 함량은 메틸자스모네이트의 처리결과 33.7% 증가하였으며, 염화나트륨의 처리로 112%가 증가하는 효과를 확인하였다. 이에 의해 본 발명에 따른 상기 화합물의 처리는 식물체내에 루틴을 포함하는 페놀화합물의 함량을 크게 증가시키는 것을 확인할 수 있고, 더욱이 이들을 함께 처리한 경우에서 그 증가폭은 이들 화합물간 상승작용에 의해 더욱 커지는 것으로 판단된다.
As a result of treatment for 1 week with buckwheat sprout using the cultivation method according to the present invention, the result of the treatment of methyl jasmonate increased the content of rutin 1.5 times or more, and the result of treating sodium chloride increased 1.8 times or more. It was. In addition, the total phenolic compound content was increased by 33.7% as a result of the treatment of methyl jasmonate, and 112% was confirmed by the treatment of sodium chloride. Thereby, the treatment of the compound according to the present invention can confirm that the content of the phenolic compound containing rutin in the plant is greatly increased, and in the case of treating them together, the increase is increased by synergy between these compounds. Judging.

이하, 본 발명의 내용을 실시예를 통해 보다 구체적으로 설명하기로 한다. 다만 이들 실시예는 본 발명의 내용을 이해하기 위한 것일 뿐 본 발명의 권리범위가 이에 의해 제한되는 것은 아니다.
Hereinafter, the content of the present invention will be described in more detail with reference to Examples. However, these embodiments are only for understanding the content of the present invention, but the scope of the present invention is not limited thereto.

[실시예 1]Example 1

15℃ 내지 18℃로 유지되는 암실에서 7일간 관수는 1일 2회, 1회처리시 20L/㎡으로 처리하고 처리수[0.1 mM 메틸자스모네이트(MeJA)]는 관수처리 중간에 1일에 1번, 1회 2L/m2 씩 메밀새싹에 처리하여 주었다In a dark room maintained at 15 ° C. to 18 ° C. for 7 days, the water is treated twice a day at 20 L / m 2 in one treatment, and the treated water [0.1 mM methyl jasmonate (MeJA)] is treated in one day in the middle of the water treatment. Once, 2L / m 2 was treated to buckwheat sprout once

메밀새싹에 대한 메틸자스모네이트의 처리결과 도 1a 내지 1d에서와 같이 무처리구에 비하여 루틴의 함량이 1.5배 정도 증가하는 것을 확인할 수 있고, 총 페놀의 함량은 무처리구에 비하여 33.7% 가량 큰 폭으로 증가하는 것을 확인할 수 있다.
As a result of the treatment of methyl jasmonate on buckwheat sprouts, as shown in FIGS. 1a to 1d, the content of rutin increased 1.5 times compared to the untreated group, and the total phenol content increased by 33.7% compared to the untreated group. You can see that.

메틸자스모네이트의 처리에 따른 항산화활성을 측정하기 위해 FRAP(ferric reducing antioxidant power) 검정법을 이용하였다. 도 2에 의하면 무처리구에 비해 메틸자스모네이트의 단독처리구에서 항산화활성이 2배 이상 증가하는 것을 확인할 수 있었다.
A ferric reducing antioxidant power (FRAP) assay was used to determine the antioxidant activity of the methyljamonate treatment. According to Figure 2 it was confirmed that the antioxidant activity increased more than two times in the single treatment of methyl jasmonate compared to the untreated.

이상에서 살펴본 바와 같이 메틸자스모네이트의 처리는 루틴을 포함하는 총 페놀함량의 증가 및 항산화활성의 증가효과가 매우 큰 것을 확인하였다.
As described above, the treatment of methyl jasmonate was confirmed that the effect of increasing the total phenol content and antioxidant activity including rutin is very large.

[실시예 2][Example 2]

15℃ 내지 18℃로 유지되는 암실에서 7일간 관수는 1일 2회, 1회처리시 20L/㎡으로 처리하고 처리수[염화나트륨(NaCl) 10, 50, 100, 200mM]는 관수처리 중간에 1일에 1번, 1회 1.6L/m2 씩 메밀새싹에 처리하여 주었다
7 days in the dark room maintained at 15 ℃ to 18 ℃ treated twice a day, 20L / ㎡ in one treatment and treated water [sodium chloride (NaCl) 10, 50, 100, 200mM] 1 in the middle of the irrigation treatment Once a day, 1.6L / m 2 was treated to buckwheat sprout once

메밀새싹에 대한 염화나트륨의 농도별 처리결과 생중량의 변화는 도 3에서와 같이 무처리구에 비하여 2.5배 이상 증가하는 것을 확인할 수 있어 염화나트륨의 처리가 생육에 좋은 영향을 미치는 것을 확인할 수 있었다.
As a result of treatment of the concentration of sodium chloride for the buckwheat sprout, the change in the raw weight was confirmed to increase by 2.5 times or more as compared to the untreated group, as shown in Figure 3, it was confirmed that the treatment of sodium chloride has a good effect on the growth.

또 메밀새싹에 대한 염화나트륨의 농도별 처리결과 도 3a에서와 같이 무처리구에 비하여 루틴의 함량이 1.8배 정도 증가하는 것을 확인할 수 있고, 도 3b에서와 같이 총 페놀의 함량은 무처리구에 비하여 112% 가량 큰 폭으로 증가하는 것을 확인할 수 있었다.
In addition, as a result of treatment of the concentration of sodium chloride in the buckwheat sprout as shown in Figure 3a it can be seen that the content of the rutin increased 1.8 times compared to the untreated group, as shown in Figure 3b the total phenolic content is about 112% larger than the untreated group It was confirmed that the width increases.

또 염화나트륨의 처리에 따른 항산화 활성을 측정하기 위해 공시된 방법에 따라 DPPH 소거활성을 측정한 결과, 도 5에서와 같이 하기 위해 무처리구에 비하여 30% 이상 증가한 것을 확인할 수 있었다.
In addition, as a result of measuring DPPH scavenging activity according to the published method to measure the antioxidant activity according to the treatment of sodium chloride, it was confirmed that increased by more than 30% compared to the untreated group as shown in FIG.

이상에서 살펴본 바와 같이 염화나트륨의 처리는 메밀싹의 생중량, 루틴을 포함하는 총 페놀함량의 증가 및 항산화활성의 증가효과가 매우 큰 것을 확인하였다.
As described above, the treatment of sodium chloride was confirmed that the effect of increasing the weight of buckwheat sprout, total phenolic content including rutin and increasing antioxidant activity was very large.

[실시예 3][Example 3]

15℃ 내지 18℃로 유지되는 암실에서 7일간 관수는 1일 2회, 1회처리시 20L/㎡으로 처리하고 처리수[100mM 염화나트륨(NaCl)+ 0.1mM 메틸자스모네이트(MeJA)]는 관수처리 중간에 1일에 1번, 1회 1.6L/m2 씩 메밀새싹에 처리하여 주었다
7 days in the dark room maintained at 15 ℃ to 18 ℃ treated twice a day, 20L / ㎡ in one treatment and treated water [100mM sodium chloride (NaCl) + 0.1mM methyl jasmonate (MeJA)] 1.6L / m 2 was treated to buckwheat sprout once a day in the middle of treatment.

메밀새싹에 대한 염화나트륨 및 메틸자스모네이트의 처리결과 생중량의 변화는 무처리구에 비하여 3.0배 이상 증가하는 것을 확인할 수 있어 염화나트륨과 메틸자스모네이트의 병행처리가 생육에 좋은 영향을 미치는 것을 확인할 수 있었다.
As a result of the treatment of sodium chloride and methyljasmonate on buckwheat sprouts, the change in fresh weight increased more than 3.0 times compared to the non-treated group, and it was confirmed that the parallel treatment of sodium chloride and methyljasmonate had a good effect on growth. .

또 메밀새싹에 대한 염화나트륨 및 메틸자스모네이트의 처리결과 무처리구에 비하여 루틴의 함량이 2.0배 정도 증가하는 것을 확인할 수 있고, 총 페놀의 함량은 무처리구에 비하여 130% 가량 큰 폭으로 증가하는 것을 확인할 수 있었다.
In addition, as a result of the treatment of sodium chloride and methyl jasmonate on buckwheat sprouts, it can be seen that the content of rutin is increased by 2.0 times compared to the untreated group, and the total phenolic content is increased by about 130% compared to the untreated group. there was.

또 염화나트륨 및 메틸자스모네이트의 처리에 따른 항산화 활성을 측정하기 위해 공시된 방법에 따라 DPPH 소거활성을 측정한 결과, 무처리구에 비하여 50% 이상 증가한 것을 확인할 수 있었다.
In addition, as a result of measuring the DPPH scavenging activity according to the published method for measuring the antioxidant activity according to the treatment of sodium chloride and methyl jasmonate, it was confirmed that the increase by more than 50% compared to the untreated group.

이상에서 살펴본 바와 같이 염화나트륨 및 메틸자스모네이트의 병행처리는 무처리구 및 이들의 단독처리구에 비하여 메밀싹의 생중량, 루틴을 포함하는 총 페놀함량의 증가 및 항산화활성의 증가효과가 매우 큰 것을 확인하였다.
As described above, the parallel treatment of sodium chloride and methyl jasmonate showed that the growth of buckwheat sprout, the total phenol content including rutin and the antioxidative activity were significantly higher than those of the untreated and monotreated groups. .

[실시예 4]Example 4

500Lux 이상으로 1일 4~12시간 정도 빛을 처리하는 것을 제외하고는 실시예 1과 동일하게 실시하였다. 그 결과 페놀성 화합물의 함량이 무처리구에 비하여 85% 이상, 메틸자스모네이트 단독으로 처리한 경우에 비하여 40% 가량 증가한 것을 확인할 수 있었다.
The same procedure as in Example 1 was conducted except that the light was treated at about 500Lux for about 4-12 hours per day. As a result, it was confirmed that the content of the phenolic compound was increased by more than 85% compared to the untreated group, 40% compared with the case treated with methyl jasmonate alone.

상기와 같이, 본 발명의 바람직한 실시 예를 참조하여 설명하였지만 해당 기술 분야의 숙련된 당업자라면 하기의 특허청구범위에 기재된 본 발명의 사상 및 영역으로부터 벗어나지 않는 범위 내에서 본 발명을 다양하게 수정 및 변경시킬 수 있음을 이해할 수 있을 것이다.
While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it will be understood by those of ordinary skill in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention as defined by the appended claims. It can be understood that

Claims (10)

메밀의 재배방법에 있어서, 염화물, 메틸자스모네이트, 또는 염화물과 메틸자스모네이트를 식물체인 메밀에 처리하여 루틴의 함량을 증가시키는 단계를 포함하는 것을 특징으로 하는 루틴의 함량이 증가된 메밀의 재배방법.A method of growing buckwheat, the method comprising increasing the content of rutin by treating chloride, methyljasmonate, or chloride and methyljasmonate with buckwheat, which is a plant, to increase the content of rutin. Cultivation method. 삭제delete 삭제delete 제 1항에 있어서,
염화물은 염화나트륨인 것을 특징으로 하는 루틴의 함량이 증가된 메밀의 재배방법. The method of claim 1,
A method of growing buckwheat with an increased content of rutin, characterized in that the chloride is sodium chloride. 제 1항에 있어서,
염화물은 재배수에 농도 10-200mM로 조절하여 식물체인 메밀에 처리되는 것을 특징으로 하는 루틴의 함량이 증가된 메밀의 재배방법. The method of claim 1,
Chloride is a method of growing buckwheat with an increase in the content of rutin, characterized in that it is treated to plant buckwheat by adjusting the concentration to 10-200mM in the cultivation water. 제 1항에 있어서,
메틸자스모네이트는 재배수에 농도 0.01-200mM로 조절하여 식물체인 메밀에 처리되는 것을 특징으로 하는 루틴의 함량이 증가된 메밀의 재배방법. The method of claim 1,
Methyl jasmonate is cultivated buckwheat cultivation method, characterized in that the treatment of buckwheat, the plant content is adjusted to a concentration of 0.01-200mM in cultivation water. 제 1항에 있어서,
재배수내 염화물과 메틸자스모네이트의 농도비는 몰농도로 100:1-1:100인 것을 특징으로 하는 루틴의 함량이 증가된 메밀의 재배방법. The method of claim 1,
Concentration ratio of chloride and methyl jasmonate in the cultivation water is cultivation method of buckwheat with an increased content of rutin, characterized in that the molar concentration of 100: 1-1: 100. 제 1항에 있어서,
메밀에 빛을 처리해 주는 단계를 더 포함하는 것을 특징으로 하는 루틴의 함량이 증가된 메밀의 재배방법.
The method of claim 1,
Method of cultivating buckwheat with increased content of the routine, characterized in that it further comprises the step of treating light to the buckwheat.
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