CN104082025A - Method for cultivating color pepper disease-resistant seedlings - Google Patents
Method for cultivating color pepper disease-resistant seedlings Download PDFInfo
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- CN104082025A CN104082025A CN201410352775.5A CN201410352775A CN104082025A CN 104082025 A CN104082025 A CN 104082025A CN 201410352775 A CN201410352775 A CN 201410352775A CN 104082025 A CN104082025 A CN 104082025A
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Abstract
The invention discloses a method for cultivating color pepper disease-resistant seedlings. According to the method, corn kernel particulate materials and bran compose a fermentation substrate with the volume ratio of 1:1, maitake mycelia are made to grow in the fermentation substrate through a solid state fermentation mode, maitake fungus fermentation broth which is rich in the maitake mycelia is obtained, the maitake fungus fermentation broth, grass carbon and vermiculite are used for preparing a seedling culturing substrate according to the volume ratio of 2:10:3, tray-soilless seedling culturing is carried out, after root systems of color pepper seedlings absorb grifolan, maitake acid and maitake elements, plants are induced to generate resistance to phytophthora root rot and other diseases, and obtained seedlings of the right age are the disease-resistant seedlings. According to the method, the problem of seedling death caused by phytophthora root rot bacteria in color pepper planting can be solved.
Description
Technical field
The present invention relates to a kind of method of cultivating the disease-resistant seedling of color green pepper, belong to growing vegetables technical field.
Background technology
Dead of color green pepper in recent years the protections such as greenhouse by solar heat occur seriously in cultivating; especially take the plantation of color green pepper year after year, be that main area occurs more; serious impact color green pepper produce, make numerous vegetable growers' interests suffer huge loss, be urgent problem during current color green pepper produces.
Color dead of green pepper is mainly after field planting by phytophthora root rot etc., to cause how to cultivate the color green pepper seedling that phytophthora root rot is had to resistance against diseases, is the common technical tasks of paying close attention to of current people.
Also about the peculiar bioactive ingredients thing of Grifolas frondosa germ being applied to color green pepper cultivation, do not strengthen at present the bibliographical information of color pepper plant resistance against diseases.
Summary of the invention
The object of the invention is the problem existing for prior art, a kind of method of cultivating the disease-resistant seedling of color green pepper is provided, by training system, be rich in the seedling medium of maitake mushroom mycelia, carry out point disk seedling growing, after color green pepper seedlings root absorbs grifolan, grifola frondosus acid and the ash tree florigen in mycelium, induce plant to produce resistance to phytophthora root rot, thereby solve in color green pepper production by phytophthora root rot microbial " dead " difficult problem.
For solving above technical problem, the technical solution used in the present invention is as follows: a kind of method of cultivating the disease-resistant seedling of color green pepper, it is characterized in that: with corn kernel shot-like particle and wheat bran, within 1: 1 by volume, form fermentation substrate, mode by solid state fermentation is grown maitake mushroom mycelia in fermentation substrate, obtain being rich in the Grifolas frondosa germ fermentation product of maitake mushroom mycelia, Grifolas frondosa germ fermentation product, the peat composed of rotten mosses and vermiculite are mixed and be mixed with seedling medium for 2: 10: 3 by volume, carry out substrate seedling, the of the right age seedling obtaining is the disease-resistant seedling of color green pepper.Peat composed of rotten mosses diameter 1～5mm, vermiculite diameter 3～5mm.
A prioritization scheme, the particle that described corn kernel shot-like particle is diameter 3～5mm size that corn kernel is ground into.
Another kind of prioritization scheme, the preparation of described Grifolas frondosa germ fermentation product comprises the following steps:
1), by after the activated cultivation of slant preservation bacterial classification, in the semi-synthetic liquid nutrient medium that access is mixed in mass ratio by potato, glucose, skim milk powder at 1000: 20: 2, cultivate 5～8 days, obtain grifola frondosus liquid spawn for 18～25 ℃;
2) pack round into after fermentation substrate and water are mixed according to the mass ratio of 4: 1～6: 1,115～132 ℃ of sterilizings 15～30 minutes after sterilizing finishes, are cooled to normal temperature under cleaning condition, standby;
3) press 10%～15% access grifola frondosus liquid spawn of fermentation substrate quality, under 20～25 ℃ of conditions, lucifuge was cultivated after 15～20 days, obtained Grifolas frondosa germ fermentation product.
Another prioritization scheme, described seedling-cultivating method is point disk seedling growing.The cave dish of selecting each hole volume to be not less than 100ml is grown seedlings, guarantee that the consumption of cultivating the above-mentioned seedling medium of 1 strain rice shoot is not less than 100ml, the matrix of getting ready is packed into after the dish of cave, by bunch planting kind, color green pepper seed of every bunch planting kind, puts into seedbed by cave dish after broadcasting and manages routinely.
Another prioritization scheme, described of the right age seedling is 3～5 leaf 1 heart seedlings.
Adopt above technical scheme, compared with prior art, have the following advantages:
(1) seedling medium of maitake mushroom mycelia is rich in the present invention by training system, carry out point disk seedling growing, color green pepper seedlings root induces plant to produce resistance material to phytophthora root rot after absorbing grifolan, grifola frondosus acid and the ash tree florigen in mycelium, can annihilate rapidly the germ of invasion, make color green pepper avoid phytophthora root rot, solved " dead " problem being caused by phytophthora root rot that is difficult to control in color green pepper plantation.
(2) the color green pepper rice shoot that the present invention cultivates is all better than existing conventional substrate seedling growing process aspect output after improving activity of root system, strong sprout index and field planting.
(3) the color green pepper rice shoot that the present invention cultivates contains multiple resistance material, not only phytophthora root rot is had to resistance, and other color green pepper disease is also had to resistance in various degree, and after field planting, damage by disease and insect occurs less, and dosage and number of times reduce, and product is safe green more.
Below in conjunction with embodiment, the invention will be further described.
Embodiment
Following examples are used for illustrating the present invention, but are not used for limiting the scope of the invention.
Embodiment 1 day-light greenhouse winter spring stubble is cultivated color green pepper, and varieties of plant is red Luo Dan, and sowing on September 5, cultivates 4 leaf 1 heart seedlings, field planting on October 15, and place is at Shouguang, Shandong green house of vegetables.
Process 1: adopt method of the present invention to carry out point disk seedling growing.Substrate composition is: Grifolas frondosa germ fermentation product, the peat composed of rotten mosses and vermiculite volume ratio 2: 10: 3, and the particle diameter 1～5mm of the peat composed of rotten mosses, the particle diameter of vermiculite is 3～5mm.
The making step of Grifolas frondosa germ fermentation product comprises: by after the activated cultivation of slant preservation bacterial classification, in the semi-synthetic liquid nutrient medium that access consists of in mass ratio potato, glucose, skim milk powder at 1000: 20: 2, cultivate 5～8 days, make grifola frondosus liquid spawn for 18～25 ℃; Corn kernel is ground into the particle of particle diameter 3～5mm size, with corn particle and wheat bran, by the volume ratio of 1: 1, is mixed into fermentation substrate; After above-mentioned fermentation substrate and water are mixed according to the mass ratio of 4: 1～6: 1, pack round into, 115～132 ℃ of sterilizings 15～30 minutes after sterilizing finishes, are cooled to normal temperature under cleaning condition, standby; 10%～15% access grifola frondosus liquid spawn of pressing fermentation substrate quality, under 20～25 ℃ of conditions, lucifuge was cultivated after 15～20 days, obtained Grifolas frondosa germ fermentation product.
Process 2: adopt the seedling medium of EM bacterium fermentation product to carry out point disk seedling growing.Substrate composition is: EM bacterium fermentation product, the peat composed of rotten mosses and vermiculite volume ratio 2: 10: 3, and the particle diameter of the peat composed of rotten mosses is 1～5mm, the particle diameter of vermiculite is 3～5mm.
The making step of EM bacterium fermentation product comprises: corn kernel is ground into the particle of particle diameter 3～5mm size, with corn particle and wheat bran, by the volume ratio of 1: 1, is mixed into fermentation substrate; With active bacterium number>=2 * 10
9the EM stoste 2.5g of cfu/g is watered 1kg mixing and is made into EM bacterium liquid, and the above-mentioned fermentation substrate of every 100kg sprays EM bacterium liquid 3kg, mixes, and adds water to water content 65%; Covered with plastic film insulation, moisturizing are carried out pile fermentation under 30～40 ℃ of conditions, during suitably breathe freely, moisturizing and stirring.After 8～10 days, after stack retting, obtain EM bacterium fermentation product.
Process 3: adopt conventional peat composed of rotten mosses seedling medium to carry out point disk seedling growing.Substrate composition is: the peat composed of rotten mosses, vermiculite volume ratio 4: 1, and the particle diameter 1～5mm of the peat composed of rotten mosses, the particle diameter of vermiculite is 3～5mm.
Above-mentioned each processed and all to be selected hole volume is that the cave dish of 100ml is grown seedlings, the consumption of every strain rice shoot matrix is 100ml, during rice shoot 3 leaf 1 heart, each is processed and gets at random 10 strains mensuration indexes in strong sprout, each is processed and gets at random 50 strain inoculation phytophthora root rot germs simultaneously, observes seedling incidence after 20 days; Field planting during 4 leaf 1 heart, statistics output, a situation arises to observe in whole vegetative period other Major Diseases.
Through test, the seedling index in strong sprout that the present invention cultivates is that 0.0369, EM bacterium fermentation product seedling medium is 0.0298, and conventional peat composed of rotten mosses seedling medium is 0.0268, the present invention has improved respectively 23.8%, 37.7% compared with EM bacterium fermentation product seedling medium, conventional peat composed of rotten mosses seedling medium, and difference reaches significance level.
The seedling inoculation phytophthora root rot germ sequela rate that the present invention cultivates is 8%, the seedling inoculation phytophthora root rot germ sequela rate that EM bacterium fermentation product seedling medium is cultivated is 90%, and the seedling inoculation phytophthora root rot germ sequela rate that conventional peat composed of rotten mosses seedling medium is cultivated is 100%.
667m after the Seeding planting that the present invention cultivates
2output is 11605.6kg, and EM bacterium fermentation product seedling medium is 9971.5kg, and conventional peat composed of rotten mosses seedling medium is 9225.1kg, and the present invention has improved respectively 16.4%, 25.8% compared with EM bacterium fermentation product seedling medium, conventional peat composed of rotten mosses seedling medium, and difference reaches significance level.
After the Seeding planting that the present invention cultivates, have no bacterial leaf spot and occur, and EM bacterium fermentation product seedling medium and the conventional peat composed of rotten mosses seedling medium bacterial leaf spot incidence of disease are respectively 8.6% and 12.5%.
After the Seeding planting that the present invention cultivates, gray mold incidence is 4.7%, and EM bacterium fermentation product seedling medium and the conventional peat composed of rotten mosses seedling medium gray mold incidence of disease are respectively 11.5% and 17.1%.
Embodiment 2 greenhouse by solar heats are got over summer stubble and are cultivated color green pepper, and varieties of plant is that century is red, and sowing on May 5, cultivates 4 leaf 1 heart seedlings, field planting on June 10, and place is at Shouguang, Shandong green house of vegetables.
Process 1: adopt method of the present invention to carry out point disk seedling growing.Substrate composition is: Grifolas frondosa germ fermentation product, the peat composed of rotten mosses and vermiculite volume ratio 2: 10: 3, and the particle diameter 1～5mm of the peat composed of rotten mosses, the particle diameter of vermiculite is 3～5mm.
The making step of Grifolas frondosa germ fermentation product comprises: by after the activated cultivation of slant preservation bacterial classification, in the semi-synthetic liquid nutrient medium that access consists of in mass ratio potato, glucose, skim milk powder at 1000: 20: 2, cultivate 7 days, make grifola frondosus liquid spawn for 20 ℃; Corn kernel is ground into the particle of particle diameter 3～5mm size, with corn particle and wheat bran, by the volume ratio of 1: 1, is mixed into fermentation substrate; After above-mentioned fermentation substrate and water are mixed according to the mass ratio of 5: 1, pack round into, 120 ℃ of sterilizings 25 minutes after sterilizing finishes, are cooled to normal temperature under cleaning condition, standby; The 12% access grifola frondosus liquid spawn of pressing fermentation substrate quality, under 25 ℃ of conditions, lucifuge was cultivated after 15 days, obtained Grifolas frondosa germ fermentation product.
Process 2: adopt the seedling medium of EM bacterium fermentation product to carry out point disk seedling growing.Substrate composition is: EM bacterium fermentation product, the peat composed of rotten mosses and vermiculite volume ratio 2: 10: 3, and the particle diameter of the peat composed of rotten mosses is 1～5mm, the particle diameter of vermiculite is 3～5mm.
The making step of EM bacterium fermentation product comprises: corn kernel is ground into the particle of particle diameter 3～5mm size, with corn particle and wheat bran, by the volume ratio of 1: 1, is mixed into fermentation substrate; With active bacterium number>=2 * 10
9the EM stoste 2.5g of cfu/g is watered 1kg mixing and is made into EM bacterium liquid, and the above-mentioned fermentation substrate of every 100kg sprays EM bacterium liquid 3kg, mixes, and adds water to water content 65%; Covered with plastic film insulation, moisturizing are carried out pile fermentation under 30～40 ℃ of conditions, during suitably breathe freely, moisturizing and stirring.After 8～10 days, after stack retting, obtain EM bacterium fermentation product.
Process 3: adopt conventional peat composed of rotten mosses seedling medium to carry out point disk seedling growing.Substrate composition is: the peat composed of rotten mosses, vermiculite volume ratio 4: 1, and the particle diameter of the peat composed of rotten mosses is 1～5mm, the particle diameter of vermiculite is 3～5mm.
Above-mentioned each processed and all to be selected hole volume is that the cave dish of 100ml is grown seedlings, the consumption of every strain rice shoot matrix is 100ml, during rice shoot 3 leaf 1 heart, each is processed and gets at random 10 strains mensuration improving activity of root systems, each is processed and gets at random 50 strain inoculation phytophthora root rot germs simultaneously, observes seedling incidence after 20 days; Field planting during 4 leaf 1 heart, statistics output, a situation arises to observe in whole vegetative period other Major Diseases.
Through test, the seedlings root vigor that the present invention cultivates is 84.46mgg
-1h
-1, EM bacterium fermentation product seedling medium is 78.69mgg
-1h
-1, conventional peat composed of rotten mosses seedling medium is 71.52mgg
-1h
-1, the present invention has improved respectively 7.3%, 18.1% compared with EM bacterium fermentation product seedling medium, conventional peat composed of rotten mosses seedling medium, and difference reaches significance level.
The seedling inoculation phytophthora root rot germ sequela rate that the present invention cultivates is 12%, the seedling inoculation phytophthora root rot germ sequela rate that EM bacterium fermentation product seedling medium is cultivated is 98%, and the seedling inoculation phytophthora root rot germ sequela rate that conventional peat composed of rotten mosses seedling medium is cultivated is 100%.
667m after the Seeding planting that the present invention cultivates
2output is 6157.2kg, and EM bacterium fermentation product seedling medium is 5391.8kg, and conventional peat composed of rotten mosses seedling medium is 4893.5kg, and the present invention has improved respectively 14.2%, 25.8% compared with EM bacterium fermentation product seedling medium, conventional peat composed of rotten mosses seedling medium, and difference reaches significance level.
After the Seeding planting that the present invention cultivates, mosaic virus incidence is 2.8%, and EM bacterium fermentation product seedling medium and conventional peat composed of rotten mosses seedling medium mosaic virus incidence are respectively 7.5% and 11.2%.
Claims (5)
1. a method of cultivating the disease-resistant seedling of color green pepper, it is characterized in that: with corn kernel shot-like particle and wheat bran, within 1: 1 by volume, form fermentation substrate, mode by solid state fermentation is grown maitake mushroom mycelia in fermentation substrate, obtain being rich in the Grifolas frondosa germ fermentation product of maitake mushroom mycelia, by 2: 10: 3 by volume even mixed preparing seedling mediums of Grifolas frondosa germ fermentation product, the peat composed of rotten mosses and vermiculite, carry out substrate seedling, the of the right age seedling obtaining is the disease-resistant seedling of color green pepper.
2. method according to claim 1, is characterized in that: the particle that described corn kernel shot-like particle is diameter 3～5mm size that corn kernel is ground into.
3. method according to claim 1, is characterized in that: the preparation of described Grifolas frondosa germ fermentation product comprises the following steps:
1), by after the activated cultivation of slant preservation bacterial classification, in the semi-synthetic liquid nutrient medium that access is mixed in mass ratio by potato, glucose, skim milk powder at 1000: 20: 2, cultivate 5～8 days, obtain grifola frondosus liquid spawn for 18～25 ℃;
2) pack round into after fermentation substrate and water are mixed according to the mass ratio of 4: 1～6: 1,115～132 ℃ of sterilizings 15～30 minutes after sterilizing finishes, are cooled to normal temperature under cleaning condition, standby;
3) press 10%～15% access grifola frondosus liquid spawn of fermentation substrate quality, under 20～25 ℃ of conditions, lucifuge was cultivated after 15～20 days, obtained Grifolas frondosa germ fermentation product.
4. method according to claim 1, is characterized in that: described seedling-cultivating method is point disk seedling growing.
5. method according to claim 1, is characterized in that: described of the right age seedling is 3～5 leaf 1 heart seedlings.
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Cited By (3)
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| CN105052515A (en) * | 2015-08-28 | 2015-11-18 | 胡永军 | Cultivation method for healthy color pepper seedlings |
| CN106008027A (en) * | 2016-06-30 | 2016-10-12 | 江苏挚信花卉发展有限公司 | Green environment-friendly nutrient solution beneficial to plant growth |
| CN107517834A (en) * | 2015-08-28 | 2017-12-29 | 寿光市新世纪种苗有限公司 | A kind of breeding method of tomato health seedling |
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Cited By (4)
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| CN105052515A (en) * | 2015-08-28 | 2015-11-18 | 胡永军 | Cultivation method for healthy color pepper seedlings |
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