CN107750850A - A kind of breeding method of color green pepper health seedling - Google Patents
A kind of breeding method of color green pepper health seedling Download PDFInfo
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- CN107750850A CN107750850A CN201710814450.8A CN201710814450A CN107750850A CN 107750850 A CN107750850 A CN 107750850A CN 201710814450 A CN201710814450 A CN 201710814450A CN 107750850 A CN107750850 A CN 107750850A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B17/00—Other phosphatic fertilisers, e.g. soft rock phosphates, bone meal
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D9/00—Other inorganic fertilisers
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- Cultivation Of Plants (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The present invention provides a kind of breeding method of color green pepper health seedling, will be enriched in Cordceps militaris bacterium fermentate, turf, vermiculite and the perlite of scarlet caterpiller fungus mycelia by volume 1：5：2：2 uniform mixed preparing seedling mediums, carry out hole plate seedling growth；Color green pepper sprout to cotyledon deploy when every plant pour that to apply concentration be 30mgkg‑1The 8ml of the potassium phosphite aqueous solution 5；It is 20mgkg that 1st lobus cardiacus dew point, which pours to every plant during 11 heart of leaf and apply concentration,‑1The 10ml of the Prohexadione calcium aqueous solution 8；Every plant is poured that to apply concentration be 100mgkg during 21 heart of leaf‑1The 15ml of the toxic fluoride phosphate aqueous solution 12；Every plant is poured that to apply concentration be 75mgkg during 31 heart of leaf‑1The 15ml of the potassium phosphite aqueous solution 12.The rice shoot that the present invention is brought out is better than conventional hole plate seedling growth in root activity, seedling vigorous index, bud differentiation, disease resistance and yield etc..
Description
The present invention is application number 201510538653X, applying date 2015-08-28, a kind of title " training of color green pepper health seedling
Educate method " divisional application.
Technical field
The present invention relates to a kind of breeding method of color green pepper seedling, specifically, is related to a kind of method for cultivating color green pepper health seedling,
Belong to vegetables seedling-raising technique field.
Background technology
Color green pepper hole plate seedling growth is widely used to produce.Hole plate seedling growth is to make nursery base with light materials such as turf, vermiculites
Matter, using precision drilling, the modernization nursery system of once-seedling forming.Traditional hole plate seedling growth technology has energy-conservation, saving of labor, province
Power, seedbed area is saved, economize on kind, be easy to long distance transportation to also allow for using the advantages that standardizing seedling management technology, it is fixed
Plant easily survives, no seedling-slowing stage, and nursery production realizes specialized, mechanization, and commercialization is realized for seedling.
In process of the present invention is realized, inventor has found problems with the prior art at least be present：Traditional color green pepper cave
Disk seedling-raising technique causes the quality of seedlings to decline, main table because the factors such as hole plate seedling growth environment tend to cause rice shoot excessive growth
Now poor for the rooting ability after rice shoot field planting, growth potential is weak, and malformed fruit rate is high（Mainly seedling stage bud differentiation is bad caused）, resist
Characteristic of disease is poor, yields poorly.
The content of the invention
The technical problems to be solved by the invention are to be directed to problem above, there is provided a kind of method for cultivating color green pepper health seedling,
Rice shoot excessive growth can effectively be suppressed, be advantageous to nurturing staff, promote root system development, improve root activity and seedling vigorous index；Promote flower
Bud well breaks up, and reduces malformed fruit rate, improves the commodity of fruit；Strengthen the disease resistance of rice shoot, reduce the use of chemical pesticide
Number and dosage；Improve color green pepper yield.
To solve the above problems, the present invention adopts the following technical scheme that：A kind of breeding method of color green pepper health seedling, its feature
It is, comprises the following steps：
1）Color green pepper seed is sowed at the 72 hole standard hole trays equipped with the seedling medium rich in scarlet caterpiller fungus mycelia, seed germination to cotyledon
Every plant is poured that to apply concentration be 30mgkg during expansion-1Potassium phosphite aqueous solution 5-8ml；
2）It is 20mgkg that 1st lobus cardiacus dew point, which pours to every plant during 11 heart of leaf and apply concentration,-1Prohexadione calcium aqueous solution 8-10ml；
3）Every plant is poured that to apply concentration be 100mgkg during 21 heart of leaf-1Toxic fluoride phosphate aqueous solution 12-15ml；
4）Every plant is poured that to apply concentration be 75mgkg during 31 heart of leaf-1Potassium phosphite aqueous solution 12-15ml；
The seedling medium rich in scarlet caterpiller fungus mycelia by Cordceps militaris bacterium fermentate, turf, vermiculite and perlite by volume
1：5：2：2 uniform mixed preparings；
The healthy seedling is cultivated for heliogreenhouse autumn and winter stubble color green pepper, and malformed fruit rate is 0；
The Streak Disease incidence of disease is 0；
The phytophthora root rot incidence of disease is 0；
Reduce chemical pesticide to use 5 times, chemical pesticide usage amount reduces more than 40%；
667m2The more conventional hole plate seedling growth of yield improves 23.0%.
The Cordceps militaris bacterium fermentate, is prepared by following steps：
1）The particle for the diameter 3-5mm sizes that corn kernel is ground into, is made corn kernel shot-like particle；
2）With corn kernel shot-like particle and wheat bran by volume 1：1 composition fermentation substrate；
3）After the activated culture of slant preservation strain, access by potato, glucose, skimmed milk power in mass ratio 1000：20：
In the 2 semi-synthetic fluid nutrient mediums mixed, 20-28 DEG C is cultivated 10 days, produces Cordceps militaris liquid spawn；
4）By fermentation substrate and water according to 5：Load round after 1 mass ratio mixing, 115-132 DEG C sterilizes 20 minutes, goes out
After bacterium is terminated, normal temperature is cooled under cleaning condition, it is standby；
5）Cordceps militaris liquid spawn, under the conditions of 20-28 DEG C, lucifuge culture 8 days are accessed by the 10%-15% of fermentation substrate quality
Afterwards, then it is transferred under 100-150lx illumination condition and cultivates 18 days, that is, Cordceps militaris bacterium fermentate is obtained, rich in scarlet caterpiller fungus mycelia.
A kind of prioritization scheme, the full elements compounding of the appropriate full dissolubility of seedling medium addition rich in scarlet caterpiller fungus mycelia
Fertilizer, make seedling medium initial nutrient N, P2O5、K2O content is respectively：50-60mg·kg-1、20-40mg·kg-1、80-
90mg·kg-1, middle trace element is appropriate, EC values 1.0-1.5mscm-1。
Using above technical scheme, compared with prior art, there is advantages below：
（1）Rice shoot excessive growth can effectively be suppressed, be advantageous to nurturing staff, promote root system development, root activity is improved and strong sprout refers to
Number.According to experiment, the more conventional Plug seedling of educating of gained rice shoot seedling vigorous index improves 22.3%-23.7%；The more conventional hole tray of root activity is educated
Seedling improves 20.1%-22.4%.
（2）Rice shoot bud differentiation is good, is advantageous to improve the commodity of fruit.According to experiment, gained rice shoot color green pepper fruit is abnormal
Shape fruit rate is 0, and conventional Plug seedling color green pepper deformed fruit fruit rate is 9.8%-14.6%.
（3）Rice shoot disease resistance is strong, is advantageous to realize that the decrement of chemical pesticide uses in color green pepper cultivation.According to experiment, gained
Field virosis, the phytophthora root rot incidence of disease are 0 after rice shoot field planting；The incidence of disease of the diseases such as epidemic disease, bacterial leaf spot is also remote
Less than conventional hole plate seedling growth, chemical pesticide can be reduced in production using 3-5 times, chemical pesticide usage amount reduces more than 40%.
（4）Be advantageous to improve color green pepper yield.According to experiment, 667m after the field planting of gained rice shoot2The more conventional hole plate seedling growth of yield carries
High 22.8%-23.0%.
Selection and the dosage of potassium phosphite, Prohexadione calcium and toxic fluoride phosphate in the present invention, are the biological characteristics according to color green pepper
Property, and result of the test of the present inventor in color green pepper hole plate seedling growth and determine.Watering is the most frequent field of hole plate seedling growth
Operation, potassium phosphite, Prohexadione calcium and pouring for toxic fluoride phosphate are applied and can carried out with watering, do not increase recruitment.
The raw materials used present invention is to allow what is used in agricultural production, and raw materials market is on sale.
With reference to embodiment, the present invention is described in detail.
Embodiment
The present embodiment is for the ease of understanding the present invention, without the claim and core limiting the invention in any way
Content.
Embodiment 1, heliogreenhouse Autumn cultivation color green pepper, varieties of plant Wei Weier, sowing on July 6, August are determined on the 14th
Plant, place is in Shandong Shouguang green house of vegetables.If 2 processing, from 72 hole standard hole plate seedling growths, in addition to desired measure,
Other control measures are identical.
Cordceps militaris bacterium fermentate, is prepared by following steps：
1）The particle for the diameter 3-5mm sizes that corn kernel is ground into, is made corn kernel shot-like particle；
2）With corn kernel shot-like particle and wheat bran by volume 1：1 composition fermentation substrate；
3）After the activated culture of slant preservation strain, access by potato, glucose, skimmed milk power in mass ratio 1000：20：
In the 2 semi-synthetic fluid nutrient mediums mixed, 20-28 DEG C is cultivated 10 days, produces Cordceps militaris liquid spawn；
4）By fermentation substrate and water according to 5：Load round after 1 mass ratio mixing, 115-132 DEG C sterilizes 20 minutes, goes out
After bacterium is terminated, normal temperature is cooled under cleaning condition, it is standby；
5）Cordceps militaris liquid spawn, under the conditions of 20-28 DEG C, lucifuge culture 8 days are accessed by the 10%-15% of fermentation substrate quality
Afterwards, then it is transferred under 100-150lx illumination condition and cultivates 18 days, that is, obtains Cordceps militaris bacterium fermentate.
Processing 1：Using the inventive method nursery
By Cordceps militaris bacterium fermentate, turf, vermiculite and perlite by volume 1：5：2：2 uniformly mixing, while add appropriate complete
Dissolubility all-element compound fertilizer prepares the seedling medium rich in scarlet caterpiller fungus mycelia, makes seedling medium initial nutrient N, P2O5、K2O contains
Amount is respectively：50-60mg·kg-1、20-40mg·kg-1、80-90mg·kg-1, middle trace element is appropriate, EC values 1.0-
1.5ms·cm-1, color green pepper hole plate seedling growth is carried out after medium in tray；Every plant is poured and applies concentration when after planting seed germination to cotyledon deploys
For 30mgkg-1Potassium phosphite aqueous solution 5-8ml；It is 20mg that 1st lobus cardiacus dew point, which pours to every plant during 11 heart of leaf and apply concentration,
kg-1Prohexadione calcium aqueous solution 8-10ml；Every plant is poured that to apply concentration be 100mgkg during 21 heart of leaf-1Toxic fluoride phosphate aqueous solution 12-
15ml；Every plant is poured that to apply concentration be 75mgkg during 31 heart of leaf-1Potassium phosphite aqueous solution 12-15ml.
Processing 2：Conventional method nursery
By 6: 2: the 2 uniformly mixing by volume of turf, vermiculite, perlite, while add appropriate full dissolubility all-element compound fertilizer and match somebody with somebody
Seedling medium processed, make seedling medium initial nutrient N, P2O5、K2O content is respectively：50-60mg·kg-1、20-40mg·kg-1、
80-90mg·kg-1, middle trace element is appropriate, EC values 1.0-1.5mscm-1, color green pepper hole plate seedling growth is carried out after medium in tray, is pressed
Conventional method spray on plants hormone controls rice shoot excessive growth.
It is colonized during above-mentioned each processing 41 heart of leaf of rice shoot, measure root activity and seedling vigorous index before being colonized；Investigated after field planting color
The malformed fruit incidence of green pepper fruit, analyze rice shoot bud differentiation situation；Investigating field diseases, a situation arises, analyzes the disease-resistant of rice shoot
Property；Count color green pepper total output.
Through experiment, the rice shoot seedling vigorous index that the present invention cultivates is 0.0307, and the rice shoot strong sprout that conventional hole plate seedling growth is brought out refers to
Number is 0.0251, and the more conventional hole plate seedling growth of the present invention improves 22.3%, and difference reaches the level of signifiance.
The rice shoot root activity that the present invention cultivates is 81.2mgg-1·h-1, the rice shoot root system of conventional hole plate seedling growth cultivation
Vigor is 67.6mgg-1·h-1, the more conventional hole plate seedling growth of the present invention improves 20.1%, significant difference.
The rice shoot fruit fruit malformed fruit rate that the present invention cultivates is 0, and the rice shoot malformed fruit that conventional hole plate seedling growth is cultivated
Rate is 9.8%, and difference reaches the level of signifiance, illustrates that the rice shoot bud differentiation that the present invention cultivates is good.
The field Streak Disease incidence of disease is 0 after the rice shoot field planting that the present invention cultivates, and conventional hole plate seedling growth is cultivated
Rice shoot field planting after the field Streak Disease incidence of disease be 10.3%, difference reach the level of signifiance, illustrates the of the invention rice shoot cultivated
Viral diseases ability is strong.
The field phytophthora root rot incidence of disease is 0 after the rice shoot field planting that the present invention cultivates, and conventional hole plate seedling growth is cultivated
Rice shoot plant after the field phytophthora root rot incidence of disease be 4.1%, illustrate the of the invention rice shoot resistant to phytophthora root rot ability cultivated
By force.
The rice shoot disease resistance that the present invention cultivates is strong, and Field diseases are low after field planting, and chemical pesticide can be reduced in production to be made
With 5 times, chemical pesticide usage amount reduces more than 40%.
The rice shoot 667m that the present invention cultivates2Yield is 12258.3kg, the rice shoot 667m that conventional hole plate seedling growth is cultivated2Production
Measure and improve 23.0% for 9962.9kg, the more conventional hole plate seedling growth of the present invention, difference reaches the level of signifiance.
Embodiment 2, heliogreenhouse get over summer stubble cultivation color green pepper, and varieties of plant is that century is red, sowing on April 20, and June 2 is fixed
Plant, place is in Shandong Shouguang green house of vegetables.If 2 processing, from 72 hole standard hole plate seedling growths, in addition to desired measure,
Other control measures are identical.
Cordceps militaris bacterium fermentate rich in scarlet caterpiller fungus mycelia, is prepared by following steps：
1）The particle for the diameter 3-5mm sizes that corn kernel is ground into, is made corn kernel shot-like particle；
2）With corn kernel shot-like particle and wheat bran by volume 1：1 composition fermentation substrate；
3）After the activated culture of slant preservation strain, access by potato, glucose, skimmed milk power in mass ratio 1000：20：
In the 2 semi-synthetic fluid nutrient mediums mixed, 20-28 DEG C is cultivated 10 days, produces Cordceps militaris liquid spawn；
4）By fermentation substrate and water according to 5：Load round after 1 mass ratio mixing, 115-132 DEG C sterilizes 20 minutes, goes out
After bacterium is terminated, normal temperature is cooled under cleaning condition, it is standby；
5）Cordceps militaris liquid spawn, under the conditions of 20-28 DEG C, lucifuge culture 8 days are accessed by the 10%-15% of fermentation substrate quality
Afterwards, then it is transferred under 100-150lx illumination condition and cultivates 18 days, that is, obtains the Cordceps militaris bacterium rich in scarlet caterpiller fungus mycelia and ferment
Thing.
Processing 1：Using the inventive method nursery
By Cordceps militaris bacterium fermentate, turf, vermiculite and perlite by volume 1：5：2：2 uniformly mixing, while add appropriate complete
Dissolubility all-element compound fertilizer prepares the seedling medium rich in scarlet caterpiller fungus mycelia, makes seedling medium initial nutrient N, P2O5、K2O contains
Amount is respectively：50-60mg·kg-1、20-40mg·kg-1、80-90mg·kg-1, middle trace element is appropriate, EC values 1.0-
1.5ms·cm-1, color green pepper hole plate seedling growth is carried out after medium in tray；Every plant is poured and applies concentration when after planting seed germination to cotyledon deploys
For 30mgkg-1Potassium phosphite aqueous solution 5-8ml；It is 20mg that 1st lobus cardiacus dew point, which pours to every plant during 11 heart of leaf and apply concentration,
kg-1Prohexadione calcium aqueous solution 8-10ml；Every plant is poured that to apply concentration be 100mgkg during 21 heart of leaf-1Toxic fluoride phosphate aqueous solution 12-
15ml；Every plant is poured that to apply concentration be 75mgkg during 31 heart of leaf-1Potassium phosphite aqueous solution 12-15ml.
Processing 2：Conventional method nursery
By 6: 2: the 2 uniformly mixing by volume of turf, vermiculite, perlite, while add appropriate full dissolubility all-element compound fertilizer and match somebody with somebody
Seedling medium processed, make seedling medium initial nutrient N, P2O5、K2O content is respectively：50-60mg·kg-1、20-40mg·kg-1、
80-90mg·kg-1, middle trace element is appropriate, EC values 1.0-1.5mscm-1, color green pepper hole plate seedling growth is carried out after medium in tray, is pressed
Conventional method spray on plants hormone controls rice shoot excessive growth.
It is colonized during above-mentioned each processing 41 heart of leaf of rice shoot, measure root activity and seedling vigorous index before being colonized；Investigated after field planting color
The malformed fruit incidence of green pepper fruit, analyze rice shoot bud differentiation situation；Investigating field diseases, a situation arises, analyzes the disease-resistant of rice shoot
Property；Count color green pepper total output.
Through experiment, the rice shoot seedling vigorous index that the present invention cultivates is 0.0325, the rice shoot strong sprout that conventional hole plate seedling growth is cultivated
Index is 0.0264, and the more conventional hole plate seedling growth of the present invention improves 23.1%, and difference reaches the level of signifiance.
The rice shoot root activity that the present invention cultivates is 82.7mgg-1·h-1, the rice shoot root system of conventional hole plate seedling growth cultivation
Vigor is 68.1 mgg-1·h-1, the more conventional hole plate seedling growth of the present invention improves 21.4%, significant difference.
The rice shoot malformed fruit rate that the present invention cultivates is 0, and the rice shoot malformed fruit rate that conventional hole plate seedling growth is cultivated is
13.8%, difference reaches the level of signifiance, illustrates that the rice shoot bud differentiation that the present invention cultivates is good.
The field Streak Disease incidence of disease is 0 after the rice shoot field planting that the present invention cultivates, and conventional hole plate seedling growth is cultivated
Rice shoot field planting after the field Streak Disease incidence of disease be 13.9%, difference reach the level of signifiance, illustrates the of the invention rice shoot cultivated
Viral diseases ability is strong.
The field phytophthora root rot incidence of disease is 0 after the rice shoot field planting that the present invention cultivates, and conventional hole plate seedling growth is cultivated
Rice shoot plant after the field phytophthora root rot incidence of disease be 5.3%, illustrate the of the invention rice shoot resistant to phytophthora root rot ability cultivated
By force.
The field bacterial leaf spot incidence of disease is 2.5% after the rice shoot field planting that the present invention cultivates, and conventional hole plate seedling growth is trained
The field bacterial leaf spot incidence of disease is 14.3% after the rice shoot field planting brought out, and difference reaches the level of signifiance, illustrates that the present invention cultivates
Rice shoot bacteria resistance leaf spot ability it is strong.
The rice shoot disease resistance that the present invention cultivates is strong, and Field diseases are low after field planting, and chemical pesticide can be reduced in production to be made
With 4 times, chemical pesticide usage amount reduces more than 40%.
The rice shoot 667m that the present invention cultivates2Yield is 4926.8kg, the rice shoot 667m that conventional hole plate seedling growth is cultivated2Production
Measure and improve 22.8% for 4012.3kg, the more conventional hole plate seedling growth of the present invention, difference reaches the level of signifiance.
Embodiment 3, heliogreenhouse early spring stubble cultivation color green pepper, varieties of plant is tangerine West Asia, is sowed December 5, in January, the 2nd
It is colonized within 20th, place is in Shandong Shouguang green house of vegetables.If 2 processing, from 72 hole standard hole trays, except desired measure
Outside, other control measures are identical.
Cordceps militaris bacterium fermentate rich in scarlet caterpiller fungus mycelia, is prepared by following steps：
1）The particle for the diameter 3-5mm sizes that corn kernel is ground into, is made corn kernel shot-like particle；
2）With corn kernel shot-like particle and wheat bran by volume 1：1 composition fermentation substrate；
3）After the activated culture of slant preservation strain, access by potato, glucose, skimmed milk power in mass ratio 1000：20：
In the 2 semi-synthetic fluid nutrient mediums mixed, 20-28 DEG C is cultivated 10 days, produces Cordceps militaris liquid spawn；
4）By fermentation substrate and water according to 5：Load round after 1 mass ratio mixing, 115-132 DEG C sterilizes 20 minutes, goes out
After bacterium is terminated, normal temperature is cooled under cleaning condition, it is standby；
5）Cordceps militaris liquid spawn, under the conditions of 20-28 DEG C, lucifuge culture 8 days are accessed by the 10%-15% of fermentation substrate quality
Afterwards, then it is transferred under 100-150lx illumination condition and cultivates 18 days, that is, obtains the Cordceps militaris bacterium rich in scarlet caterpiller fungus mycelia and ferment
Thing.
Processing 1：Using the inventive method nursery
It will be enriched in Cordceps militaris bacterium fermentate, turf, vermiculite and the perlite of scarlet caterpiller fungus mycelia by volume 1：5：2：2 is uniformly mixed
Close, while add appropriate full dissolubility all-element compound fertilizer and prepare seedling medium, make seedling medium initial nutrient N, P2O5、K2O content
Respectively：50-60mg·kg-1、20-40mg·kg-1、80-90mg·kg-1, middle trace element is appropriate, EC values 1.0-1.5ms
cm-1, color green pepper hole plate seedling growth is carried out after medium in tray；Every plant is poured that to apply concentration be 30mg when after planting seed germination to cotyledon deploys
kg-1Potassium phosphite aqueous solution 5-8ml；It is 20mgkg that 1st lobus cardiacus dew point, which pours to every plant during 11 heart of leaf and apply concentration,-1Tune
Naphthenic acid calcium aqueous solution 8-10ml；Every plant is poured that to apply concentration be 100mgkg during 21 heart of leaf-1Toxic fluoride phosphate aqueous solution 12-15ml；3 leaves 1
Every plant is poured that to apply concentration be 75mgkg during the heart-1Potassium phosphite aqueous solution 12-15ml.
Processing 2：Conventional method nursery
By 6: 2: the 2 uniformly mixing by volume of turf, vermiculite, perlite, while add appropriate full dissolubility all-element compound fertilizer and match somebody with somebody
Seedling medium processed, make seedling medium initial nutrient N, P2O5、K2O content is respectively：50-60mg·kg-1、20-40mg·kg-1、
80-90mg·kg-1, middle trace element is appropriate, EC values 1.0-1.5mscm-1, color green pepper hole plate seedling growth is carried out after medium in tray, is pressed
Conventional method spray on plants hormone controls rice shoot excessive growth.
It is colonized during above-mentioned each processing 41 heart of leaf of rice shoot, measure root activity and seedling vigorous index before being colonized；Investigated after field planting color
The malformed fruit incidence of green pepper fruit, analyze rice shoot bud differentiation situation；Investigating field diseases, a situation arises, analyzes the disease-resistant of rice shoot
Property；Count color green pepper total output.
Through experiment, the rice shoot seedling vigorous index that the present invention cultivates is 0.0298, the rice shoot strong sprout that conventional hole plate seedling growth is cultivated
Index is 0.0241, and the more conventional hole plate seedling growth of the present invention improves 23.7%, and difference reaches the level of signifiance.
The rice shoot root activity that the present invention cultivates is 86.9mgg-1·h-1, the rice shoot root system of conventional hole plate seedling growth cultivation
Vigor is 71.0mgg-1·h-1, the more conventional hole plate seedling growth of the present invention improves 22.4%, significant difference.
The rice shoot malformed fruit rate that the present invention cultivates is 0, and the rice shoot malformed fruit rate that conventional hole plate seedling growth is cultivated is
14.6%, difference reaches the level of signifiance, illustrates that the rice shoot bud differentiation that the present invention cultivates is good.
The field mosaic virus incidence of disease is 0 after the rice shoot field planting that the present invention cultivates, and conventional hole plate seedling growth is cultivated
Rice shoot field planting after the field mosaic virus incidence of disease be 11.3%, difference reach the level of signifiance, illustrates the of the invention rice shoot cultivated
Viral diseases ability is strong.
The field phytophthora root rot incidence of disease is 0 after the rice shoot field planting that the present invention cultivates, and conventional hole plate seedling growth is cultivated
Rice shoot plant after the field phytophthora root rot incidence of disease be 6.2%, illustrate the of the invention rice shoot resistant to phytophthora root rot ability cultivated
By force.
The field epidemic disease incidence of disease is 4.3% after the rice shoot field planting that the present invention cultivates, and the seedling that conventional hole plate seedling growth is cultivated
The field epidemic disease incidence of disease is 12.9% after seedling field planting, and difference reaches the level of signifiance, illustrates the anti-epidemic disease ability of rice shoot that the present invention cultivates
By force.
The field bacterial leaf spot incidence of disease is 2.5% after the rice shoot field planting that the present invention cultivates, and conventional hole plate seedling growth is trained
The field bacterial leaf spot incidence of disease is 16.3% after the rice shoot field planting brought out, and difference reaches the level of signifiance, illustrates that the present invention cultivates
Rice shoot bacteria resistance leaf spot ability it is strong.
The rice shoot disease resistance that the present invention cultivates is strong, and Field diseases are low after field planting, and chemical pesticide can be reduced in production to be made
With 3 times, chemical pesticide usage amount reduces more than 40%.
The rice shoot 667m that the present invention cultivates2Yield is 6738.4kg, the rice shoot 667m that conventional hole plate seedling growth is cultivated2Production
Measure and improve 22.9% for 5481.5kg, the more conventional hole plate seedling growth of the present invention, difference reaches the level of signifiance.
Citing described above for best mode for carrying out the invention, wherein the part do not addressed in detail is that this area is common
The common knowledge of technical staff.Protection scope of the present invention is defined by the content of claim, any technology based on the present invention
The equivalent transformation for enlightening and carrying out, also within protection scope of the present invention.
Claims (3)
1. a kind of breeding method of color green pepper health seedling, it is characterised in that comprise the following steps：
1）Color green pepper seed is sowed at the 72 hole standard hole trays equipped with the seedling medium rich in scarlet caterpiller fungus mycelia, seed germination to cotyledon
Every plant is poured that to apply concentration be 30mgkg during expansion-1Potassium phosphite aqueous solution 5-8ml；
2）It is 20mgkg that 1st lobus cardiacus dew point, which pours to every plant during 11 heart of leaf and apply concentration,-1Prohexadione calcium aqueous solution 8-10ml；
3）Every plant is poured that to apply concentration be 100mgkg during 21 heart of leaf-1Toxic fluoride phosphate aqueous solution 12-15ml；
4）Every plant is poured that to apply concentration be 75mgkg during 31 heart of leaf-1Potassium phosphite aqueous solution 12-15ml；
The seedling medium rich in scarlet caterpiller fungus mycelia by Cordceps militaris bacterium fermentate, turf, vermiculite and perlite by volume
1：5：2：2 uniform mixed preparings；
The healthy seedling is cultivated for heliogreenhouse autumn and winter stubble color green pepper, and malformed fruit rate is 0；
The Streak Disease incidence of disease is 0；
The phytophthora root rot incidence of disease is 0；
Reduce chemical pesticide to use 5 times, chemical pesticide usage amount reduces more than 40%；
667m2The more conventional hole plate seedling growth of yield improves 23.0%.
2. the breeding method of color green pepper health seedling according to claim 1, it is characterised in that：The Cordceps militaris bacterium fermentate, lead to
Following steps are crossed to be prepared：
1）The particle for the diameter 3-5mm sizes that corn kernel is ground into, is made corn kernel shot-like particle；
2）With corn kernel shot-like particle and wheat bran by volume 1：1 composition fermentation substrate；
3）After the activated culture of slant preservation strain, access by potato, glucose, skimmed milk power in mass ratio 1000：20：
In the 2 semi-synthetic fluid nutrient mediums mixed, 20-28 DEG C is cultivated 10 days, produces Cordceps militaris liquid spawn；
4）By fermentation substrate and water according to 5：Load round after 1 mass ratio mixing, 115-132 DEG C sterilizes 20 minutes, goes out
After bacterium is terminated, normal temperature is cooled under cleaning condition, it is standby；
5）Cordceps militaris liquid spawn, under the conditions of 20-28 DEG C, lucifuge culture 8 days are accessed by the 10%-15% of fermentation substrate quality
Afterwards, then it is transferred under 100-150lx illumination condition and cultivates 18 days, that is, Cordceps militaris bacterium fermentate is obtained, rich in scarlet caterpiller fungus mycelia.
3. the breeding method of color green pepper health seedling according to claim 1, it is characterised in that：It is described rich in scarlet caterpiller fungus mycelia
The appropriate full dissolubility all-element compound fertilizer of seedling medium addition, makes seedling medium initial nutrient N, P2O5、K2O content is respectively：
50-60mg·kg-1、20-40mg·kg-1、80-90mg·kg-1, middle trace element is appropriate, EC values 1.0-1.5mscm-1。
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| CN106134704A (en) * | 2016-06-28 | 2016-11-23 | 扬州大学 | The method outputing two kinds of color flowers on the same plant of Radix Paeoniae |
| CN106472283A (en) * | 2016-10-20 | 2017-03-08 | 山东鑫诚现代农业科技有限责任公司 | A kind of glasshouse color green pepper soilless culture method |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2006191848A (en) * | 2005-01-13 | 2006-07-27 | Ajinomoto Co Inc | New method for cultivating fruit vegetable crop in undernutrition condition |
| CN102503739A (en) * | 2011-11-04 | 2012-06-20 | 广西田园生化股份有限公司 | Compound fertilizer of toxic fluorine-phosphorus and foliage fertilizer |
| CN103392575A (en) * | 2013-08-06 | 2013-11-20 | 山东省寿光蔬菜产业集团有限公司 | Multifunctional seedling cultivation substrate |
| CN104082025A (en) * | 2014-07-24 | 2014-10-08 | 胡永军 | Method for cultivating color pepper disease-resistant seedlings |
| CN104094764A (en) * | 2014-07-24 | 2014-10-15 | 胡永军 | Cultivation method for disease-resistant color pepper sprouts |
| CN104115672A (en) * | 2014-07-24 | 2014-10-29 | 巩玉升 | Cultivation method for tomato cordyceps militaris fruits |
| CN104529644A (en) * | 2014-12-31 | 2015-04-22 | 广东福利龙复合肥有限公司 | Water soluble fertilizer with function of preventing and treating citrus dieback and preparing method thereof |
| CN105052515B (en) * | 2015-08-28 | 2017-09-22 | 寿光恒蔬无疆农业发展集团有限公司 | A kind of breeding method of the healthy seedling of color green pepper |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102150531A (en) * | 2010-12-09 | 2011-08-17 | 苏州硒谷科技有限公司 | Hot pepper rich in organic selenium and production method thereof |
| CN102487708A (en) * | 2011-12-16 | 2012-06-13 | 珠海银基农产品有限公司 | Method for growing pepper |
| CN103766095A (en) * | 2012-10-18 | 2014-05-07 | 吴燕 | Pepper planting method |
| CN103460937A (en) * | 2013-09-12 | 2013-12-25 | 江苏希旺农业科技有限公司 | Color pepper cultivation technology |
-
2015
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Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2006191848A (en) * | 2005-01-13 | 2006-07-27 | Ajinomoto Co Inc | New method for cultivating fruit vegetable crop in undernutrition condition |
| CN102503739A (en) * | 2011-11-04 | 2012-06-20 | 广西田园生化股份有限公司 | Compound fertilizer of toxic fluorine-phosphorus and foliage fertilizer |
| CN103392575A (en) * | 2013-08-06 | 2013-11-20 | 山东省寿光蔬菜产业集团有限公司 | Multifunctional seedling cultivation substrate |
| CN104082025A (en) * | 2014-07-24 | 2014-10-08 | 胡永军 | Method for cultivating color pepper disease-resistant seedlings |
| CN104094764A (en) * | 2014-07-24 | 2014-10-15 | 胡永军 | Cultivation method for disease-resistant color pepper sprouts |
| CN104115672A (en) * | 2014-07-24 | 2014-10-29 | 巩玉升 | Cultivation method for tomato cordyceps militaris fruits |
| CN104529644A (en) * | 2014-12-31 | 2015-04-22 | 广东福利龙复合肥有限公司 | Water soluble fertilizer with function of preventing and treating citrus dieback and preparing method thereof |
| CN105052515B (en) * | 2015-08-28 | 2017-09-22 | 寿光恒蔬无疆农业发展集团有限公司 | A kind of breeding method of the healthy seedling of color green pepper |
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