CN104024404A - 单倍体细胞 - Google Patents

单倍体细胞 Download PDF

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Publication number
CN104024404A
CN104024404A CN201280065785.XA CN201280065785A CN104024404A CN 104024404 A CN104024404 A CN 104024404A CN 201280065785 A CN201280065785 A CN 201280065785A CN 104024404 A CN104024404 A CN 104024404A
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Prior art keywords
cells
haploid
cell
embryonic stem
stem cells
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Pending
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CN201280065785.XA
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English (en)
Chinese (zh)
Inventor
U·艾林
J·彭宁格
J·陶贝茨米德
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IMBA Institut fur Molekulare Biotechonologie GmbH
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IMBA Institut fur Molekulare Biotechonologie GmbH
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Publication of CN104024404A publication Critical patent/CN104024404A/zh
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/025—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/04—Antibacterial agents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5014—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing toxicity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2503/00—Use of cells in diagnostics
    • C12N2503/02—Drug screening
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/106—Pharmacogenomics, i.e. genetic variability in individual responses to drugs and drug metabolism

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
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  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Immunology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Toxicology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Developmental Biology & Embryology (AREA)
  • Gynecology & Obstetrics (AREA)
  • Reproductive Health (AREA)
  • Cell Biology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biophysics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Pathology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • General Physics & Mathematics (AREA)
  • Oncology (AREA)
CN201280065785.XA 2011-11-30 2012-11-30 单倍体细胞 Pending CN104024404A (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
EP11191408.1 2011-11-30
EP11191408.1A EP2599859A1 (en) 2011-11-30 2011-11-30 Haploid cells
US201161565626P 2011-12-01 2011-12-01
US61/565,626 2011-12-01
PCT/EP2012/074112 WO2013079670A1 (en) 2011-11-30 2012-11-30 Haploid cells

Publications (1)

Publication Number Publication Date
CN104024404A true CN104024404A (zh) 2014-09-03

Family

ID=45218335

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201280065785.XA Pending CN104024404A (zh) 2011-11-30 2012-11-30 单倍体细胞

Country Status (11)

Country Link
US (1) US20140342369A1 (enExample)
EP (2) EP2599859A1 (enExample)
JP (1) JP2015500637A (enExample)
KR (1) KR20140101390A (enExample)
CN (1) CN104024404A (enExample)
AU (1) AU2012343826A1 (enExample)
CA (1) CA2856810A1 (enExample)
IL (1) IL232879A0 (enExample)
IN (1) IN2014KN01374A (enExample)
SG (1) SG11201402350QA (enExample)
WO (1) WO2013079670A1 (enExample)

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107217042A (zh) * 2017-07-31 2017-09-29 江苏东抗生物医药科技有限公司 一种生产无岩藻糖基化蛋白的基因工程细胞系及其建立方法
CN108138138A (zh) * 2015-07-29 2018-06-08 纽约干细胞基金会有限公司 单倍体人胚胎干细胞系和体细胞系及其制备方法
CN108998410A (zh) * 2017-06-07 2018-12-14 中国科学院动物研究所 蛋白激酶抑制剂在抑制单倍体细胞二倍化中的用途
CN109689894A (zh) * 2016-06-19 2019-04-26 耶路撒冷希伯来大学伊萨姆研发有限公司 在人单倍体细胞中筛选化学疗法抗性
CN118370275A (zh) * 2024-06-21 2024-07-23 中国科学院昆明动物研究所 一种产生母源性半克隆小鼠的方法
CN118389411A (zh) * 2024-06-21 2024-07-26 中国科学院昆明动物研究所 一种小鼠双单倍体胚胎干细胞分离建系的方法

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9957479B2 (en) 2011-03-02 2018-05-01 Anton Wutz Mammalian haploid embryonic stem cells
WO2015152146A1 (ja) * 2014-03-31 2015-10-08 国立大学法人東京医科歯科大学 １倍体胚性幹細胞の培養方法
JP7026304B2 (ja) 2016-07-29 2022-02-28 マックス－プランク－ゲゼルシャフト ツア フェルデルンク デア ヴィッセンシャフテン エー．ファウ． 部位特異的ｄｎａ開裂及び修復による標的化原位置タンパク質多様化
EP3388530A1 (en) 2017-04-13 2018-10-17 IMBA-Institut für Molekulare Biotechnologie GmbH Nucleic acid modification and identification method
ES3043187T3 (en) 2017-04-13 2025-11-25 Imba Inst Molekulare Biotech Nucleic acid modification and identification method
US10626319B1 (en) 2019-05-17 2020-04-21 King Fahd University Of Petroleum And Minerals Methods of inhibiting corrosion with a pyrazine corrosion inhibitor
EP4183873A4 (en) * 2020-07-17 2024-04-24 Tohoku University Trophoblast stem cell-like cells capable of differentiating into placenta-constituting cells, and method for producing same

Citations (1)

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CN101715485A (zh) * 2007-04-06 2010-05-26 国际干细胞公司 源于人单性生殖胚泡的患者特异性干细胞系

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US5945577A (en) 1997-01-10 1999-08-31 University Of Massachusetts As Represented By Its Amherst Campus Cloning using donor nuclei from proliferating somatic cells
EP1092768A1 (en) 1999-10-16 2001-04-18 ARTEMIS Pharmaceuticals GmbH Conditional gene trapping construct for the disruption of genes
CN100497598C (zh) 1999-10-28 2009-06-10 马萨诸塞大学 多能细胞和细胞系的单雌生殖或单雄生殖产生,及其产生分化细胞和组织的用途
AU1581001A (en) 1999-11-02 2001-05-14 University Of Massachusetts Use of haploid genomes for genetic diagnosis, modification and multiplication
US7074611B2 (en) 2001-04-27 2006-07-11 Gie-Cerbn, Centre Europeen De Recherche En Biologie Et En Medecine (Gie) Method for the stable inversion of DNA sequence by site-specific recombination and DNA vectors and transgenic cells thereof
AU2003297700A1 (en) 2002-12-09 2004-06-30 Boehringer Ingelheim Pharmaceuticals, Inc. METHODS FOR MODULATING IKKAlpha ACTIVITY
HUE028026T2 (en) 2002-12-16 2016-11-28 Technion Res & Dev Foundation Breeding system free of native cells, free of foreign matter for human embryonic stem cells
EP1662005A1 (en) 2004-11-26 2006-05-31 FrankGen Biotechnologie AG Enhancer-containing gene trap vectors for random and targeted gene trapping
DK1815001T3 (da) 2004-11-26 2011-05-16 Helmholtz Zentrum Muenchen Genfældekassetter til tilfældig og målrettet konditioneret geninaktivering
US20100069251A1 (en) 2006-09-15 2010-03-18 Children's Medical Center Corporation Methods for producing embryonic stem cells from parthenogenetic embryos
US9957479B2 (en) * 2011-03-02 2018-05-01 Anton Wutz Mammalian haploid embryonic stem cells

Patent Citations (1)

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CN101715485A (zh) * 2007-04-06 2010-05-26 国际干细胞公司 源于人单性生殖胚泡的患者特异性干细胞系

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KAUFMAN MH 等: "Establishment of pluripotential cell lines from haploid mouse embryos", 《J EMBRYOL EXP MORPHOL.》 *
MARTIN LEEB 等: "Derivation of haploid embryonic stem cells from mouse embryos", 《NATURE》 *
MARTIN LEEB 等: "Derivation of haploid embryonic stem cells from mouse embryos", 《NATURE》, 7 September 2011 (2011-09-07) *
WU T 等: "Reprogramming of trophoblast stem cells into pluripotent stem cells by Oct4", 《STEM CELLS》 *
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Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108138138A (zh) * 2015-07-29 2018-06-08 纽约干细胞基金会有限公司 单倍体人胚胎干细胞系和体细胞系及其制备方法
CN108138138B (zh) * 2015-07-29 2023-11-14 纽约干细胞基金会有限公司 单倍体人胚胎干细胞系和体细胞系及其制备方法
US12091678B2 (en) 2015-07-29 2024-09-17 New York Stem Cell Foundation, Inc. Haploid human embryonic stem cell lines and somatic cell lines and methods of making the same
CN109689894A (zh) * 2016-06-19 2019-04-26 耶路撒冷希伯来大学伊萨姆研发有限公司 在人单倍体细胞中筛选化学疗法抗性
CN108998410A (zh) * 2017-06-07 2018-12-14 中国科学院动物研究所 蛋白激酶抑制剂在抑制单倍体细胞二倍化中的用途
CN108998410B (zh) * 2017-06-07 2020-11-06 中国科学院动物研究所 蛋白激酶抑制剂在抑制单倍体细胞二倍化中的用途
CN107217042A (zh) * 2017-07-31 2017-09-29 江苏东抗生物医药科技有限公司 一种生产无岩藻糖基化蛋白的基因工程细胞系及其建立方法
CN107217042B (zh) * 2017-07-31 2020-03-06 江苏东抗生物医药科技有限公司 一种生产无岩藻糖基化蛋白的基因工程细胞系及其建立方法
CN118370275A (zh) * 2024-06-21 2024-07-23 中国科学院昆明动物研究所 一种产生母源性半克隆小鼠的方法
CN118389411A (zh) * 2024-06-21 2024-07-26 中国科学院昆明动物研究所 一种小鼠双单倍体胚胎干细胞分离建系的方法

Also Published As

Publication number Publication date
EP2785832A1 (en) 2014-10-08
IL232879A0 (en) 2014-07-31
US20140342369A1 (en) 2014-11-20
AU2012343826A1 (en) 2014-06-12
KR20140101390A (ko) 2014-08-19
CA2856810A1 (en) 2013-06-06
SG11201402350QA (en) 2014-06-27
WO2013079670A1 (en) 2013-06-06
IN2014KN01374A (enExample) 2015-10-16
JP2015500637A (ja) 2015-01-08
EP2599859A1 (en) 2013-06-05

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Application publication date: 20140903