CN103998606B - 修饰的肠激酶轻链 - Google Patents

修饰的肠激酶轻链 Download PDF

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Publication number
CN103998606B
CN103998606B CN201280063896.7A CN201280063896A CN103998606B CN 103998606 B CN103998606 B CN 103998606B CN 201280063896 A CN201280063896 A CN 201280063896A CN 103998606 B CN103998606 B CN 103998606B
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China
Prior art keywords
light chain
asp
enterokinase light
peptide
bovine enterokinase
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CN201280063896.7A
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English (en)
Chinese (zh)
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CN103998606A (zh
Inventor
H.F.维迪克
张旭家
刘云
佟崴嵬
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Novo Nordisk AS
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Novo Nordisk AS
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21009—Enteropeptidase (3.4.21.9), i.e. enterokinase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
CN201280063896.7A 2011-12-23 2012-12-20 修饰的肠激酶轻链 Active CN103998606B (zh)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201280063896.7A CN103998606B (zh) 2011-12-23 2012-12-20 修饰的肠激酶轻链

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
CN2011002169 2011-12-23
CNPCT/CN2011/002169 2011-12-23
CN201280063896.7A CN103998606B (zh) 2011-12-23 2012-12-20 修饰的肠激酶轻链
PCT/EP2012/076372 WO2013092855A1 (en) 2011-12-23 2012-12-20 Modified enterokinase light chain

Publications (2)

Publication Number Publication Date
CN103998606A CN103998606A (zh) 2014-08-20
CN103998606B true CN103998606B (zh) 2018-04-17

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
CN201280063896.7A Active CN103998606B (zh) 2011-12-23 2012-12-20 修饰的肠激酶轻链

Country Status (6)

Country Link
US (1) US9611466B2 (enExample)
EP (1) EP2794875B1 (enExample)
JP (1) JP6138151B2 (enExample)
CN (1) CN103998606B (enExample)
ES (1) ES2819288T3 (enExample)
WO (1) WO2013092855A1 (enExample)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3083701B1 (en) * 2013-12-17 2019-08-07 Novo Nordisk A/S Enterokinase cleavable polypeptides
CN104911166A (zh) * 2014-12-15 2015-09-16 上海张江生物技术有限公司 一种新型重组鳉鱼肠激酶轻链蛋白质
WO2017118752A1 (en) * 2016-01-07 2017-07-13 Novo Nordisk A/S Modified enterokinase light chain and its preparation method
CN108265042A (zh) * 2016-12-30 2018-07-10 江苏万邦生化医药集团有限责任公司 一种重组肠激酶的制备方法
CN109136209B (zh) * 2018-07-18 2021-07-06 上海雅心生物技术有限公司 肠激酶轻链突变体及其应用
CN114774397B (zh) * 2022-06-20 2022-10-04 北京惠之衡生物科技有限公司 牛肠激酶轻链蛋白突变体及重组融合蛋白
CN114807101B (zh) * 2022-06-20 2022-09-16 北京惠之衡生物科技有限公司 包含牛肠激酶轻链蛋白的融合蛋白及其表达载体和重组工程菌

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1470634A (zh) * 2002-07-26 2004-01-28 刘建宁 一种具有高活性和高稳定性的肠激酶轻链变体

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU675506B2 (en) 1993-01-15 1997-02-06 Genetics Institute, Llc Cloning of enterokinase and method of use
KR19990073067A (ko) 1999-03-13 1999-10-05 문정복 레일천공기
KR100507980B1 (ko) 2002-06-18 2005-08-17 프로테온 주식회사 엔테로키나제 경사슬의 아미노말단 또는 카르복실말단이 변형된 재조합 엔테로키나제
CN1869236A (zh) 2005-05-24 2006-11-29 上海新生源医药研究有限公司 一种重组牛肠激酶的生产方法
WO2008136014A1 (en) 2007-05-07 2008-11-13 Usv Limited Cloning and expression of enterokinase and its process for production
CN101624588B (zh) 2007-05-11 2012-02-22 上海抗体药物国家工程研究中心有限公司 重组牛肠激酶轻链突变体
CN102061302B (zh) 2010-11-26 2012-07-04 扬子江药业集团北京海燕药业有限公司 肠激酶轻链基因的合成及其表达产物的制备方法

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1470634A (zh) * 2002-07-26 2004-01-28 刘建宁 一种具有高活性和高稳定性的肠激酶轻链变体

Also Published As

Publication number Publication date
ES2819288T3 (es) 2021-04-15
US20150064744A1 (en) 2015-03-05
JP2015502167A (ja) 2015-01-22
EP2794875B1 (en) 2020-07-22
JP6138151B2 (ja) 2017-05-31
WO2013092855A1 (en) 2013-06-27
EP2794875A1 (en) 2014-10-29
CN103998606A (zh) 2014-08-20
US9611466B2 (en) 2017-04-04

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