ES2819288T3 - Cadena ligera de enteroquinasa modificada - Google Patents
Cadena ligera de enteroquinasa modificada Download PDFInfo
- Publication number
- ES2819288T3 ES2819288T3 ES12816050T ES12816050T ES2819288T3 ES 2819288 T3 ES2819288 T3 ES 2819288T3 ES 12816050 T ES12816050 T ES 12816050T ES 12816050 T ES12816050 T ES 12816050T ES 2819288 T3 ES2819288 T3 ES 2819288T3
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- Spain
- Prior art keywords
- light chain
- analog
- enterokinase
- enterokinase light
- protein
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6424—Serine endopeptidases (3.4.21)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/21—Serine endopeptidases (3.4.21)
- C12Y304/21009—Enteropeptidase (3.4.21.9), i.e. enterokinase
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/50—Fusion polypeptide containing protease site
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
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- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011002169 | 2011-12-23 | ||
| PCT/EP2012/076372 WO2013092855A1 (en) | 2011-12-23 | 2012-12-20 | Modified enterokinase light chain |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2819288T3 true ES2819288T3 (es) | 2021-04-15 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES12816050T Active ES2819288T3 (es) | 2011-12-23 | 2012-12-20 | Cadena ligera de enteroquinasa modificada |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US9611466B2 (enExample) |
| EP (1) | EP2794875B1 (enExample) |
| JP (1) | JP6138151B2 (enExample) |
| ES (1) | ES2819288T3 (enExample) |
| WO (1) | WO2013092855A1 (enExample) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP6200087B2 (ja) * | 2013-12-17 | 2017-09-20 | ノヴォ ノルディスク アー／エス | エンテロキナーゼ切断性ポリペプチド |
| WO2017118752A1 (en) * | 2016-01-07 | 2017-07-13 | Novo Nordisk A/S | Modified enterokinase light chain and its preparation method |
| CN109136209B (zh) * | 2018-07-18 | 2021-07-06 | 上海雅心生物技术有限公司 | 肠激酶轻链突变体及其应用 |
| CN114807101B (zh) * | 2022-06-20 | 2022-09-16 | 北京惠之衡生物科技有限公司 | 包含牛肠激酶轻链蛋白的融合蛋白及其表达载体和重组工程菌 |
| CN116064491A (zh) * | 2022-06-20 | 2023-05-05 | 北京惠之衡生物科技有限公司 | 牛肠激酶轻链蛋白突变体及其重组融合蛋白 |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0679189B1 (en) | 1993-01-15 | 2003-06-04 | Genetics Institute, LLC | Cloning of enterokinase and method of use |
| KR19990073067A (ko) | 1999-03-13 | 1999-10-05 | 문정복 | 레일천공기 |
| KR100507980B1 (ko) | 2002-06-18 | 2005-08-17 | 프로테온 주식회사 | 엔테로키나제 경사슬의 아미노말단 또는 카르복실말단이 변형된 재조합 엔테로키나제 |
| CN1214107C (zh) | 2002-07-26 | 2005-08-10 | 刘建宁 | 一种具有高活性和高稳定性的肠激酶轻链变体 |
| CN1869236A (zh) | 2005-05-24 | 2006-11-29 | 上海新生源医药研究有限公司 | 一种重组牛肠激酶的生产方法 |
| WO2008136014A1 (en) | 2007-05-07 | 2008-11-13 | Usv Limited | Cloning and expression of enterokinase and its process for production |
| CN101624587A (zh) | 2007-05-11 | 2010-01-13 | 上海张江生物技术有限公司 | 重组牛肠激酶、其制备方法及用途 |
| CN102061302B (zh) | 2010-11-26 | 2012-07-04 | 扬子江药业集团北京海燕药业有限公司 | 肠激酶轻链基因的合成及其表达产物的制备方法 |
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- 2012-12-20 WO PCT/EP2012/076372 patent/WO2013092855A1/en not_active Ceased
- 2012-12-20 EP EP12816050.4A patent/EP2794875B1/en active Active
- 2012-12-20 US US14/364,464 patent/US9611466B2/en active Active
- 2012-12-20 JP JP2014548013A patent/JP6138151B2/ja active Active
- 2012-12-20 ES ES12816050T patent/ES2819288T3/es active Active
Also Published As
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|---|---|
| US20150064744A1 (en) | 2015-03-05 |
| EP2794875B1 (en) | 2020-07-22 |
| US9611466B2 (en) | 2017-04-04 |
| JP2015502167A (ja) | 2015-01-22 |
| EP2794875A1 (en) | 2014-10-29 |
| WO2013092855A1 (en) | 2013-06-27 |
| JP6138151B2 (ja) | 2017-05-31 |
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