JP6138151B2 - 修飾されたエンテロキナーゼ軽鎖 - Google Patents

修飾されたエンテロキナーゼ軽鎖 Download PDF

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Publication number
JP6138151B2
JP6138151B2 JP2014548013A JP2014548013A JP6138151B2 JP 6138151 B2 JP6138151 B2 JP 6138151B2 JP 2014548013 A JP2014548013 A JP 2014548013A JP 2014548013 A JP2014548013 A JP 2014548013A JP 6138151 B2 JP6138151 B2 JP 6138151B2
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Japan
Prior art keywords
light chain
enterokinase light
bovine enterokinase
protein
bovine
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JP2014548013A
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English (en)
Japanese (ja)
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JP2015502167A5 (enExample
JP2015502167A (ja
Inventor
ヘレ・ファブリーシュス・ヴルディゲ
シュジア・ジャン
ユン・リウ
ウェイウェイ・トン
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Novo Nordisk AS
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Novo Nordisk AS
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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21009—Enteropeptidase (3.4.21.9), i.e. enterokinase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
JP2014548013A 2011-12-23 2012-12-20 修飾されたエンテロキナーゼ軽鎖 Active JP6138151B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
CNPCT/CN2011/002169 2011-12-23
CN2011002169 2011-12-23
PCT/EP2012/076372 WO2013092855A1 (en) 2011-12-23 2012-12-20 Modified enterokinase light chain

Publications (3)

Publication Number Publication Date
JP2015502167A JP2015502167A (ja) 2015-01-22
JP2015502167A5 JP2015502167A5 (enExample) 2016-02-12
JP6138151B2 true JP6138151B2 (ja) 2017-05-31

Family

ID=47561561

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2014548013A Active JP6138151B2 (ja) 2011-12-23 2012-12-20 修飾されたエンテロキナーゼ軽鎖

Country Status (5)

Country Link
US (1) US9611466B2 (enExample)
EP (1) EP2794875B1 (enExample)
JP (1) JP6138151B2 (enExample)
ES (1) ES2819288T3 (enExample)
WO (1) WO2013092855A1 (enExample)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6200087B2 (ja) * 2013-12-17 2017-09-20 ノヴォ ノルディスク アー／エス エンテロキナーゼ切断性ポリペプチド
WO2017118752A1 (en) * 2016-01-07 2017-07-13 Novo Nordisk A/S Modified enterokinase light chain and its preparation method
CN109136209B (zh) * 2018-07-18 2021-07-06 上海雅心生物技术有限公司 肠激酶轻链突变体及其应用
CN114807101B (zh) * 2022-06-20 2022-09-16 北京惠之衡生物科技有限公司 包含牛肠激酶轻链蛋白的融合蛋白及其表达载体和重组工程菌
CN116064491A (zh) * 2022-06-20 2023-05-05 北京惠之衡生物科技有限公司 牛肠激酶轻链蛋白突变体及其重组融合蛋白

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0679189B1 (en) 1993-01-15 2003-06-04 Genetics Institute, LLC Cloning of enterokinase and method of use
KR19990073067A (ko) 1999-03-13 1999-10-05 문정복 레일천공기
KR100507980B1 (ko) 2002-06-18 2005-08-17 프로테온 주식회사 엔테로키나제 경사슬의 아미노말단 또는 카르복실말단이 변형된 재조합 엔테로키나제
CN1214107C (zh) 2002-07-26 2005-08-10 刘建宁 一种具有高活性和高稳定性的肠激酶轻链变体
CN1869236A (zh) 2005-05-24 2006-11-29 上海新生源医药研究有限公司 一种重组牛肠激酶的生产方法
WO2008136014A1 (en) 2007-05-07 2008-11-13 Usv Limited Cloning and expression of enterokinase and its process for production
CN101624587A (zh) 2007-05-11 2010-01-13 上海张江生物技术有限公司 重组牛肠激酶、其制备方法及用途
CN102061302B (zh) 2010-11-26 2012-07-04 扬子江药业集团北京海燕药业有限公司 肠激酶轻链基因的合成及其表达产物的制备方法

Also Published As

Publication number Publication date
US20150064744A1 (en) 2015-03-05
EP2794875B1 (en) 2020-07-22
US9611466B2 (en) 2017-04-04
ES2819288T3 (es) 2021-04-15
JP2015502167A (ja) 2015-01-22
EP2794875A1 (en) 2014-10-29
WO2013092855A1 (en) 2013-06-27

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