CN103989692B - Method for preparing compound amino acid and ginseng saponin capsule - Google Patents
Method for preparing compound amino acid and ginseng saponin capsule Download PDFInfo
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Abstract
The invention discloses a method for preparing a compound amino acid and ginseng saponin capsule. The method comprises the following steps: (1), wherein the capsule is prepared from the following raw materials in parts by weight: 1-2 parts of compound ginseng saponin and 416-832 parts of compound amino acid, wherein the compound amino acid is prepared from the following components by mass percent: 1.22% of histidine, 1.02% of isoleucine, 1.43% of leucine, 1.22% of lysine, 1.32% of methionine, 0.51% of tyrosine, 0.81% of phenylalanine, 0.36% of threonine, 1.02% of valine, 14.58% of alanine, 11.93% of arginine, 13.16% of aspartic acid, 5.3% of glycine, 26.11% of glutamic acid, 6.63% of proline and 3.66% of serine; the compound ginseng saponin is prepared from the following components by mass percent: 14.8% of Rgl, 11.73% of Re, 28.21% of Rbl, 1.40% of Rg2, 20.11% of Rc, 16.48% of Rb2 and 7.26% of Rd; (2) fully mixing the compound amino acid with the compound ginseng saponin evenly according to the raw materials in the step (2), carrying out dry heat sterilization at 100 DEG C for an hour, and enclosing into capsules after sterilizing, so as to prepare the product.
Description
Technical field
The present invention relates to a kind of compound amino acid ginsenoside capsule preparation method thereof, category modernization of cmm field.
Background technology
Various ginsenosides have the effect for alleviating physical fatigue, and ginsenoside Rg1 has can reduce internal lactic acid
Pile up, and lactic acid is the product of body anaerobic exercise, and body can be made to feel fatigue, ginsenoside Rg1 reduces the accumulation of lactic acid, from
And reach the effect for alleviating physical fatigue.The research such as Wang finds that Ginsenosides Rg1 and Rb1 can alleviate central nervous system of rats fatigue,
Its principle is that two kinds of saponin(es can strengthen acetylcholine transferase activity, increased acetylcholine synthesis and content.In addition, also grinding
Studying carefully proves that ginseng sapoglycoside Rg 3 and Rh2 have the effect for alleviating physical fatigue.Ginsenoside Re also has the work for alleviating organism fatigue
With Feng Yi Chong etc. obtains exercise induced fatigue model by experimental animal modeling of SD rats, the common 14d of ginsenoside Re is administered, then
The SOD activity and mda content of each tissue are determined, the administration mouse liver of ginsenoside Re, muscle and red is as a result found
The SOD activity of cell is significantly higher than model control group, liver, muscle and the content of MDA is significantly lower than model comparison in serum
Group, thus proves that the mechanism that ginsenoside Re alleviates organism fatigue is to improve the SOD activity of liver, muscle and red blood cell, drop
Low liver, muscle and in serum MDA content.Separately there are some researches show ginsenoside Re can reach improvement for different target spots
Central neurotransmitter is disorderly caused by exercise induced fatigue, so as to reach the effect for resisting and alleviating exercise induced fatigue.Ginsenoside
Composite nutrient can also be constituted with other combinations of substances, so as to play the employments such as its effect for alleviating physical fatigue, Ou Yiyi
Ginseng saponin(e and Bee Pollen, B family vitamin prescription, study it and alleviate physical fatigue effect, as a result prove that the complex nutrient preparation has
There is alleviation physical fatigue to act on.Ginsenoside Rg1 and 1,6-diphosphofructose prescription are studied it and alleviate muscle power by Chen Linjun etc.
The effect of fatigue, as a result proves, it has certain protective effect to mitochondrial breathing, can alleviate cell hypoxia damage,
Alleviate the effect of physical fatigue so as to reach.
Not only there is ginsenoside significant physical fatigue of alleviating to act on, and it strengthens immune effect also highly significant, such as
Cavy is injected with the mixture of antigen Staphylococcus aureus with ginsenoside Rbl, and it is proved with antibody titer experiment
Can also demonstrate that ginsenoside Rbl has immunoadjuvant function with strengthen immunity, the experiment to milk cow, can improve special in animal body
Heterotypic immunity antibody mediated immunity activity.The research such as Sun finds that ginsenoside Rd has very strong immunoadjuvant function, can make egg white egg
White immune mouse humoral and cellular immune response is remarkably reinforced.Yang Le etc. also found what ginsenoside Rd can cause to SNI in body
Excessive immune behavior carries out certain alleviation, so that the quick anomaly of postoperative pain obtains certain alleviation.And author is also
The mechanism for illustrating ginsenoside Rd this behavior may be the expression that it can effectively reduce NK-1 and SP acceptors in NTS.Separately
There is Kong Xiangfeng etc. and carried out ginsenoside with chicken to improve immune, as a result show that it can reduce chicken internal antibody dense
Spend the speed for declining.Report ginseng extract can strengthen cellular immune function to Scaglione etc. and Concha etc. respectively.
The pharmacological action of amino acid is often embodied in the aspects such as its nutrition, immune and alleviation physical fatigue to body, many
Plant the nutriment with amino acid as main component and embody the significant effect for alleviating physical fatigue and strengthen immunity, such as Ah
Glue, stichopus japonicus, royal jelly, hippocampus, tortoise, mare's milk, cervus elaphus linnaeus blood, silkworm chrysalis and medical and edible dual purpose plant maca etc..But, with regard to amino acid
From the point of view of the whole trend of research, mixed amino acid will quickly replace natural amino acid medicine and food, and Ru a Zhao Jun man of virtue and ability is led to
Cross zoopery and demonstrate mixed amino acid with significant alleviation physical fatigue effect.Amino acid is arranged in pairs or groups with other materials
Situation it is also of common occurrence, such as Xiao Lining, Chen Jianwei etc. have studied the alleviation physical fatigue of amino acid, vitamine preparation respectively
Effect, as a result finds no matter form of medication is capsule or electuary, and amino acid, vitamine preparation can be to the rat of exhausted movemeat
Produce obvious alleviation physical fatigue effect.Zhang Rongquan etc. proves that the calcium preparation of kilnitamin has and alleviates physical fatigue work
With.
Histidine can also strengthen the antioxidant ability of organism and specific immunity of juvenile jian carp.Guo Jun has found clearly process
CMC-Na and the coated leucine of starch can significantly increase the specific immunity and non-specific immunity of down producing goat.Zhao Chunrong
Then find that lysine is added in the feed of juvenile jian carp can significantly improve its immunologic function.Wang Guanghua has found the feed in juvenile jian carp
Middle addition egg nitronic acid is significantly increased to the identification of pathogen, adhesion, suppression and killing ability.Handsome Ke Faxian synthetic methionines
Juvenile jian carp disease-fighting capabilities can be remarkably reinforced.Tryptophan can improve the resistance against diseases of animal, additionally it is possible to control Animal stress
Reaction, so as to reduce the generation of attack.The nitrogen that arginine can significantly reduce body is lost, beneficial to the hair of albumen synthesis activity
It is raw, so that human body immune function is adjusted, strengthen immunity.Glycine has liver protection, suppresses tumour growth.
It can be seen that, ginsenoside is respectively provided with extensive pharmacological activity with amino acid, alleviates sports fatigue on the two and is immunized
Activity research in terms of regulation turns into study hotspot already.But compound amino acid ginsenoside helps to alleviate sports fatigue, has
There is not been reported to help strengthen immunity research, and the present invention is to mix people's participation compound amino acid in certain proportion, is ground
Study carefully the optimum proportioning and consumption of compound amino acid gen-seng, compound amino acid ginsenoside has convenient to take, effect compared with folk prescription
Fruit is more preferably, production is simple, low cost, advantage easy to utilize.
The content of the invention
A kind of compound amino acid ginsenoside capsule preparation method thereof of example 1
The present invention is a kind of compound amino acid ginsenoside capsule preparation method thereof, and the compound amino acid ginsenoside capsule has
Help alleviate sports fatigue, contribute to strengthen immunity, with convenient to take, better, production is simple, low cost, be easy to
The advantage of popularization and application.
Compound amino acid ginsenoside capsule of the present invention is made up of following raw material：The weight portion of compound amino acid 416 ~ 832, it is multiple
Close the weight portion of ginsenoside 1 ~ 2；Wherein, compound amino acid is in mass ratio by 1.22% histidine, 1.02% isoleucine, 1.43%
Leucine, 1.22% lysine, 1.32% methionine, 0.51% tyrosine, 0.81% phenylalanine, 0.36% threonine, 1.02% figured silk fabrics
Propylhomoserin, 14.58% alanine, 11.93% arginine, 13.16% aspartic acid, 5.3% glycine, 26.11% glutamic acid, 6.63% dried meat
Propylhomoserin, 3.66% serine composition；Compound ginsenoside by mass percentage by 14.8%Rg1,11.73%Re, 28.21%Rb1,
1.40%Rg2,20.11%Rc, 16.48%Rb2,7.26%Rd are constituted.Compound amino acid ginsenoside capsule of the present invention is by following step
It is rapid to complete：
1st, prepared by compound amino acid：1.22% histidine, 1.02% isoleucine, 1.43% bright ammonia are accurately weighed in mass ratio
Acid, 1.22% lysine, 1.32% methionine, 0.51% tyrosine, 0.81% phenylalanine, 0.36% threonine, 1.02% valine,
14.58% alanine, 11.93% arginine, 13.16% aspartic acid, 5.3% glycine, 26.11% glutamic acid, 6.63% proline,
3.66% serine, it is well mixed with mixer.
2nd, ginsenoside is combined to prepare：14.8%Rg1,11.73%Re, 28.21%Rb1,1.40% are accurately weighed in mass ratio
Rg2,20.11%Rc, 16.48%Rb2,7.26%Rd, it is well mixed with mixer.
3rd, prepared by compound amino acid ginsenoside：Accurately weighed by compound amino acid ginsenoside capsule raw material composition compound
Amino acid and compound ginsenoside, 100 DEG C hot air sterilization 1hs well mixed with mixer, glue is filled after sterilizing with capsule filling machine
Capsule is made this product, every capsule 300mg.
Influence of the compound amino acid ginsenoside of test example 1 to sports fatigue
1 materials and methods
1.1 experiment materials
Compound amino acid ginsenoside capsule is provided by research department of Ginseng In Jilin Province engineering center, wherein, the weight of ginsenoside 1
Amount part, the weight portion of compound amino acid 416.Healthy male mice in kunming, 7 week old, body weight 19-22g is provided by Jilin University.
Liquaemin（Chinese Hui Shi biochemical reagents company）；Full blood lactic kit（Bioengineering Research Institute, lot number are built up in Nanjing：
20120910）；Urea nitrogen kit（Bioengineering Research Institute, lot number are built up in Nanjing：20120907）；Hepatic glycogen kit（South
Bioengineering Research Institute, lot number are built up in capital：20120912）；Muscle glycogen kit（Bioengineering Research Institute, lot number are built up in Nanjing：
20120917）.
1.2 animal packets and dosage
Mouse 48 is chosen, 4 groups are randomly divided into, every group 12, first group (I) is blank control group, gastric infusion physiology
Salt solution；Second group (II) is compound amino acid ginsenoside low dose group, gastric infusion low dosage compound amino acid ginsenoside
3.0 mg/0.4mL/d；3rd group (III) is compound amino acid ginsenoside middle dose group, gastric infusion low dosage compounded amino
The mg/0.4mL/d of sour ginsenoside 15；4th group (IV) is compound amino acid ginsenoside high dose group, high dose of gastric infusion
The mg/0.4mL/d of amount compound amino acid sun-dried ginseng 75；Compound amino acid ginsenoside is suspended in 1% sodium carboxymethylcellulose
Daily gastric infusion 0.4mL, administration time 4 weeks.
1.3 mice burden swimming tests
Gastric infusion 4 weeks, weighs before last dose to mouse, and prepares the galvanized wire equivalent to each Mouse Weight 10%,
After 30min, galvanized wire is twined in mouse tail, be placed in the square swimming trunk of 50*40*40cm, depth of water 30cm, 30 ± 1 DEG C of water temperature,
Timing when mouse enters water, record mouse exhausts from swimming to power is started（5 s under water, head can not float）Time,
Then mouse is taken out from water immediately, and chaeta is dried with filter paper, put back to mouse cage.
The measure of 1.4 full blood lactics (BLA)
By Loaned swimming test mouse, precondition is identical therewith is further continued for gastric infusion 2 days, in the min of last gavage 30
Afterwards, mouse is put in the min of swimming cylinder went swimming 40 of depth of water 30cm, wait after 15 min that rest, plucks eyeball blood sampling, and make blood equal
In the even centrifuge tube instilled through heparin immersion, specific operation process is entered according to the measure kit specification of full blood lactic (BLA)
OK.
The measure of 1.5 serum urea nitrogens (BUN)
By Loaned swimming test mouse, precondition is identical therewith is further continued for gastric infusion 2 days, in after the min of last gavage 30,
Mouse is put in the min of swimming cylinder went swimming 40 of depth of water 30cm, wait after 15 min that rest, eyeball blood sampling is plucked, serum is taken for serum
The measure of urea nitrogen.Specific operation process is carried out according to serum urea nitrogen kit specification.
The measure of 1.6 hepatic glycogen, muscle glycogen
Eyeball takes the mouse of cervical dislocation execution immediately after blood, and rapid solution takes mouse liver and hindlimb muscle, is placed in frost
In physiological saline, connective tissue is rejected, blotted with filter paper, weighed, kit measurement hepatic glycogen is determined with muscle glycogen and hepatic glycogen
And muscle glycogen content.
1.7 data statistics
It is analyzed using the statistical softwares of SPSS 18.0, experimental result method for expressing is the shape of mean+SD
Formula, group difference compares uses one-way analysis of variance, is carried out with least significant difference value method (LSD) again when difference is statistically significant
Compare two-by-two, conspicuousness standard is p<0.05.
2 experimental results
Influence of the 2.1 compound amino acid ginsenosides to sports fatigue
Compound amino acid ginsenoside extends the mice burden swimming time, reduces Mouse whole blood lactic acid and serum urea
The content of nitrogen；The hepatic glycogen and muscle glycogen content of mouse are improve, 1 is shown in Table.It can be seen that, compound amino acid ginsenoside helps to delay
Solution sports fatigue.
Influence of the compound amino acid ginsenoside of table 1 to sports fatigue（X ± s, n=12, unit：mmol/L)
| Group | The mice burden swimming time（S） | Blood lactase acid | Serum urea nitrogen | Hepatic glycogen | Muscle glycogen |
| Ⅰ | 461.84±40.56a | 8.90±0.71c | 10.57±0.97c | 3.89±0.32a | 1.51±0.09a |
| Ⅱ | 843.33±76.98c | 3.74±0.21b | 6.95±0.21ab | 6.52±0.24c | 1.99±0.05c |
| Ⅲ | 940.25±92.38d | 1.37±0.11a | 6.06±0.33a | 7.32±0.52d | 2.31±0.21d |
| Ⅳ | 563.82±49.33b | 3.98±0.22b | 7.08±0.62ab | 4.73±0.21b | 1.73±0.13b |
Note：Compare two-by-two between each group, the not notable P of alphabetical same difference>0.05, letter difference significant difference P<0.05.
Influence of the compound amino acid ginsenoside of experimental example 2 to immunologic function
1 experiment material and method
1.1 medicines and reagent
Compound amino acid ginsenoside capsule is provided by research department of Ginseng In Jilin Province engineering center, wherein, the weight of ginsenoside 1
Amount part, the weight portion of compound amino acid 416.Mouse interleukin 2（IL-2）Enzyme-linked immunoassay kit（The U.S.
Biosource companies, lot number：20120915）；Morine IgE (IgE) enzyme-linked immunoassay kit（The U.S.
Biosource companies, lot number：20120914）；Mouse immuning ball protein G1 (IgG1) enzyme-linked immunoassay kit（The U.S.
Biosource companies, lot number：20120912）；Mouse immuning ball protein M (IgM) enzyme-linked immunoassay kit（The U.S.
Biosource companies, lot number：20120915）；Murine tumor necrosis factor α（TNF-α）Enzyme-linked immunoassay kit（The U.S.
Biosource companies, lot number：20120912）.
1.2 experimental techniques
1.2.1 experimental animal and its raising
Healthy male cleaning mouse, 7 week old, body weight 19-22g is provided by Jilin University；Raised based on all of feed
Material, is all provided by Jilin University；Enough mouse grain and drinking water is provided, ingestion types are free choice feeding；There is provided 12/12 hour
Light dark cycles（Lighting hours is early 6 points to late 6 points）, room temperature maintains about 24 ± 1 DEG C, and 50 ± 5%, wind speed is 14- to humidity
16cm/s。
1.2.2 experimental animal modeling, packet and dosage
Experimental animal is adapted to raise 1 week in laboratory condition and related behavioral indexes are trained, choose body
Focus on 29-31g, locomitivity and the mouse 88 of average level no significant difference are randomly divided into 6 groups, except blank control group and
Each 20 of model control group is outer, other every group 12.In addition to blank control group, to remaining each group mouse peritoneal injection endoxan
50mg/kg, prepares the low model of mouse immune, daily injection 1 time, continuous injection 2 days, randomly selects blank control group and the positive
Each 8 of control group, detects its organ index and LC, there is significant difference with blank control group（P ＜ 0.05）, say
Bright modeling success.
Gastric infusion 0.4mL daily to each group mouse, administration time 4 weeks after modeling success.First group (I) is blank
Group, second group of (II) model control group, this two groups are administered physiological saline；3rd group (III) is Western medicine positive controls, gavage
Administration levamisol 0.72mg/0.4mL/d；4th group (IV) is compound amino acid ginsenoside low dose group, and gastric infusion is low
The mg/0.4mL/d of dosage compound amino acid ginsenoside 3.0；5th group (V) is compound amino acid ginsenoside middle dose group,
The mg/0.4mL/d of gastric infusion low dosage compound amino acid ginsenoside 15；6th group (VI) is compound amino acid ginsenoside
High dose group, the mg/0.4mL/d of gastric infusion high dose compound amino acid sun-dried ginseng 75；Compound amino acid ginsenoside is suspended in
Daily gastric infusion 0.4mL, administration time 4 weeks in 1% sodium carboxymethylcellulose.
1.2.3 influence of the compound amino acid ginsenoside to immune organ
Mouse is weighed after last dose 20min, mouse then is plucked into eyeball takes blood, and the mouse after blood sampling takes off cervical vertebra
Put to death, and take out the spleen and thymus gland of mouse rapidly, after rejecting other connective tissues related on internal organs, directly accurately weigh dirty
The amount of thinking highly of.Organ index computing formula：Organ index（%）=(Organ weight (mg)/weight (g)).
1.2.4 influence of the compound amino acid ginsenoside to immunocyte and cell factor
1.2.4.1 compound amino acid ginsenoside is to SPL subgroup CD4+、CD8+Influence
In superclean bench, aseptic taking-up mouse spleen adds 10 mL precooling PBS liquid, and the aseptic spleen taken is put
Put online in the mesh of aperture 200, ground with syringe nook closing member, filtration is washed twice with cold PBS and is made single cell suspension.Cytometer
Number makes its final concentration be at least up to 1 × 106cells/mL.Draw the μ L of splenocyte suspension 100 and add centrifuge tube, add 1 μ L's
CD4+- FITC and 2 μ L CD8+- PE, lucifuge is incubated 30 min after mixing.Flow cytometer dedicated pipe is added with PBS washing and filterings
In, finally use flow cytomery CD4+And CD8+T cell positive expression rate.
Compound amino acid Ginsenoside on mice interleukin 2（IL-2）, tumor necrosis factor α（TNF-α）Influence
The mouse blood that eyeball taking-up will be plucked stands prepared serum after centrifugation, and it is white to determine mouse by corresponding reagent box specification
Cytokine 2（IL-2）, tumor necrosis factor α（TNF-α）.
1.2.5 influence of the compound amino acid ginsenoside to humoral immunity
The mouse blood that eyeball taking-up will be plucked stands prepared serum after centrifugation, and determining mouse by corresponding reagent box specification exempts from
Epidemic disease globulin E (IgE), mouse immuning ball protein G1（IgG1）, immunoglobulin M（IgM）.
1.2.6 data statistics
It is analyzed using the statistical softwares of SPSS 17.0, experimental result method for expressing is the shape of mean+SD
Formula, group difference compares uses one-way analysis of variance, is carried out with least significant difference value method (LSD) again when difference is statistically significant
Compare two-by-two, conspicuousness standard is p<0.05.
2 experimental results
Influence of the 2.1 compound amino acid ginsenosides to immune organ
Compound amino acid ginsenoside significantly improves the index and spleen index of endoxan immunodeficiency models mouse, thymus gland and refers to
Number, is shown in Table 2.It can be seen that, compound amino acid ginsenoside serves protective effect to immune organ.
Influence of the compound amino acid ginsenoside of table 2 to immune organ（X ± s, n=12, mg/g）
| Group | Index and spleen index | Thymus index |
| Ⅰ | 5.87±0.17c | 2.71±0.12b |
| Ⅱ | 3.20±0.13a | 1.59±0.07a |
| Ⅲ | 5.54±0.15bc | 2.98±0.04b |
| Ⅳ | 5.99±0.23c | 2.74±0.09b |
| Ⅴ | 6.69±0.13d | 2.82±0.08b |
| Ⅵ | 5.02±0.09b | 2.69±0.12b |
Note：Compare two-by-two between each group, the not notable P of alphabetical same difference>0.05, letter difference significant difference P<0.05.
2. influence of the 2 compound amino acid ginsenosides to immunocyte and cell factor
Compound amino acid ginsenoside significantly improves the CD4 of endoxan immunodeficiency models mouse+Cell quantity, together
When also improve the content of IL-2 and TNF-α.It can be seen that compound amino acid ginsenoside enhances the function of cellular immunity.
Influence of the compound amino acid ginsenoside of table 3 to immunocyte and cell factor（X ± s, n=12）
| Group | CD4+（%） | CD8+（%） | CD4+/CD8+ | IL-2（ng/L） | TNF-α（ng/L） |
| Ⅰ | 6.26±0.06a | 3.10±0.35a | 2.06±0.056a | 1111.23±27.95b | 373.26±18.60b |
| Ⅱ | 11.87±0.78d | 3.40±0.26ab | 3.51±0.90d | 146.58±9.60a | 117.50±11.53a |
| Ⅲ | 10.37±0.31c | 4.10±0.36c | 2.55±0.62b | 1138.17±61.39b | 398.64±38.64b |
| Ⅳ | 8.80±0.72b | 3.40±0.47ab | 2.59±0.10b | 1194.56±41.78bc | 367.96±21.12b |
| Ⅴ | 12.73±0.51e | 5.37±0.71d | 2.34±0.36b | 1297.93±69.84c | 375.53±5.33b |
| Ⅵ | 10.17±0.77c | 3.60±0.34bc | 2.98±0.62c | 1137.16±5.40b | 119.32±1.27a |
Note：Compare two-by-two between each group, the not notable P of alphabetical same difference>0.05, letter difference significant difference P<0.05.
Influence of the 2.3 compound amino acid ginsenosides to humoral immunity
As shown in Table 4, compound amino acid ginsenoside significantly improve endoxan immunodeficiency models mouse IgE,
IgM, IgG content.It can be seen that compound amino acid ginsenoside enhances the function of mouse humoral immune.
Influence of the compound amino acid ginsenoside of table 4 to humoral immunity（X ± s, n=12）
| Group | IgE(ng/mL) | IgG (μg /mL) | IgM(IU/mL) |
| Ⅰ | 608.63±51.42a | 118.22±10.45c | 144.64±5.94c |
| Ⅱ | 911.24±97.37b | 77.31±6.89a | 29.25±2.13a |
| Ⅲ | 2210.92±148.68d | 117.84±6.64c | 144.00±4.22c |
| Ⅳ | 2065.16±20.10d | 104.95±2.23b | 142.42±4.92c |
| Ⅴ | 1585.43±44.61c | 105.72±7.74b | 156.32±4.34c |
| Ⅵ | 2735.10±97.22e | 82.35±5.06a | 120.31±5.54b |
Note：Compare two-by-two between each group, the not notable P of alphabetical same difference>0.05, letter difference significant difference P<0.05.
Claims (1)
1. a kind of compound amino acid ginsenoside capsule, it is characterised in that completed by following steps：
(1) compound amino acid ginsenoside capsule is made up of following raw material：The compound weight portion of ginsenoside 1~2, compound amino acid
416~832 weight portions；
(2) by compound amino acid and compound ginsenoside, it is sufficiently mixed uniformly by the raw material composition in above-mentioned (1), 100 DEG C xeothermic
Sterilizing 1h, compound amino acid ginsenoside is encapsulated after sterilizing is made this product；
Described compounded amino acid starting material：By mass percentage by 1.22% histidine, 1.02% isoleucine, 1.43% bright ammonia
Acid, 1.22% lysine, 1.32% methionine, 0.51% tyrosine, 0.81% phenylalanine, 0.36% threonine, 1.02%
Valine, 14.58% alanine, 11.93% arginine, 13.16% aspartic acid, 5.3% glycine, 26.11% glutamic acid,
6.63% proline, 3.66% serine composition；
Described compound ginsenoside raw material：By mass percentage by 14.8%Rg1,11.73%Re, 28.21%Rb1,
1.40%Rg2,20.11%Rc, 16.48%Rb2,7.26%Rd are constituted.
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Effective date of registration: 20201126 Address after: Yongling town Jiahe village, Xinbin Manchu Autonomous County, Fushun City, Liaoning Province Patentee after: China agriculture Heyu (Liaoning) Agricultural Technology Co., Ltd Address before: 130118 Changchun City, Jilin Metro Street, No. 2888 Patentee before: JILIN AGRICULTURAL University |