CN103190630B - A kind of functional protein powder containing conjugated linoleic acid glyceride - Google Patents
A kind of functional protein powder containing conjugated linoleic acid glyceride Download PDFInfo
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Abstract
The present invention relates to functional food technical field, be specifically related to a kind of functional protein powder containing conjugated linoleic acid glyceride, its formula comprises 50 ~ 88 parts of soybean isolate protein powders, 5 ~ 30 parts of PURE WHEYs, 3 ~ 20 parts of conjugated linoleic acid glyceride microcapsule powders, 1 ~ 10 part of powdered phospholipid.The conjugated linoleic acid glyceride composition added in formula, can not only reduce cholesterol and antiatherosclerosis, thus contributes to reducing blood fat, also has the effect improving immunity, improve nutrient distribution, control body weight.This day by day improves for along with living standard, and the crowd occurring overnutrition or there is overnutrition risk provides a kind of functional food that can prevent and improve the various health care functions such as rich man's disease.
Description
Technical field
The present invention relates to functional food technical field, be specifically related to a kind of functional protein powder containing conjugated linoleic acid glyceride.
Background technology
Modern society's people's rhythm of life is more and more faster, the various factors such as operating pressure, diet are unbalanced cause human body to be in sub-health state, particularly due to long-term edible oil and fat content is high, heat is high food, amount of exercise reduces, and causes the various symptoms such as overnutrition, as rich man's disease.Current rich man's disease has become the main diseases kind of harm compatriots health.According to statistics, China the elderly 15 ~ 20% constipation, sixty percent is white collar; According to Ministry of Public Health's investigation, in state-owned 22% people overweight, more than 6,000 ten thousand people seek medical advice because of fat, hypertension people more than 200,000,000, diabetes more than 5,000 ten thousand people, high fat of blood 1.6 hundred million people.The whole nation causes death toll more than 1.5 ten thousand due to " rich man's disease " every day, account for more than 70% of total toll, because the expense of rich man's disease treatment accounts for more than 60% of Disease Spectrum, comprise the western countries such as the U.S., Britain medical personnel and also repeatedly deliver survey report, the development of China's rich man's disease that affluence is got up is felt concern, estimates that rich man's disease in 2015 is more widely current in China.Thus prevent the meaning of this overnutrition of rich man's disease or nutritious superfluous risk equally important with extra-nutrition, become the focus of research.
The nutrition regulation teacher that protein powder comes from American-European countries the earliest strengthens the research of the aspect such as locomitivity, fatigue-relieving to sportsman, movement characteristic according to human body cell, tissue, muscle is born, main component is protein, protein to immunity of building up health, improve, keep full of vitality and all play an important role.Because of its Be very effective, instant, gets most of the attention, and is being once that many national sports team must not disclosed wuwa.Along with the reach of science, nutritionists update protein powder, produce applicable crowd's scope novel nourishing food more widely gradually, and it is extended to common people house from sports team.The primary raw material of current protein powder is on the market mostly soybean isolate protein powder and/or PURE WHEY, be equipped with phosphatide, polysaccharide, mineral matter, vitamin, soya-bean polypeptides, or add the essence such as chocolate flavoured, fruity, vanillic aldehyde on a small quantity, or add the materials such as sweetener, such as: Amway Nutrilite protein powder, its primary raw material is soybean protein isolate, concentrated milk protein, lecithin, silica; Soup minister is strong protein powder doubly, its primary raw material: these protein powders of soybean protein isolate, lactalbumin, soybean lecithin, vegetable fat powder; Unlimited pole protein powder, soybean protein isolate, malt flour, soy peptide powder, maltodextrin, silica, wheat fragrant powdered flavor, acesulfame potassium etc., the formula of above-mentioned various protein powder is relatively simple, its effect mainly supplements the nutritional labelings such as albumen to improve physique, fatigue-relieving, enhancing physical efficiency etc., health-care efficacy is relatively single, particularly not for the protein powder of the overnutrition of modern society's appearance, prevention effect of this rich man's disease of Hyperlipemia.
Scientific research finds, CLA is a kind of natural mixed active material be present in organism, safe, nontoxic, have and improve the effect such as immunity, reduction cholesterol, antiatherosclerosis, raising skeleton density, loss of weight, can be used for the health food preparing prevention rich man's disease.Be rich in CLA Yoghourt and preparation method as Chinese invention patent (CN101731335A) discloses one, Chinese invention patent (CN1686532A) discloses a kind of people to lose weight nutrient containing CLA.But CLA is unstable, as being processed into Powdered and adding in food then complicated process of preparation to, and there is certain astringent taste and smell, mouthfeel poor being unfavorable for eats, thus need one badly can substitute CLA and have and improve nutrient distribution in human body, auxiliary antilipemic, rich man's disease can be prevented to have building up health simultaneously, improve the multiple efficacies such as immunity, and be convenient for people to reconstitute edible functional protein powder.But the relevant report that at home and abroad there is no at present about the protein powder containing conjugated linoleic acid glyceride.
Summary of the invention
The object of the invention is to avoid weak point of the prior art and a kind of functional protein powder containing conjugated linoleic acid glyceride is provided.
Object of the present invention is achieved through the following technical solutions:
There is provided a kind of functional protein powder containing conjugated linoleic acid glyceride, described protein powder is made up of the raw material of following weight portion:
Soybean isolate protein powder 50 ~ 88 parts
PURE WHEY 5 ~ 30 parts
Conjugated linoleic acid glyceride microcapsule powder 3 ~ 20 parts
Powder phospholipid 1 ~ 10 part;
Wherein, described powder phospholipid is the powder mixture be mixed by 30% lecithin, 24% cephalin and 16% cuorin;
The preparation method of described protein powder:
1) weigh:
Each raw material is taken respectively according to above-mentioned formula ratio;
2) slightly mix
Powdered phospholipid and conjugated linoleic acid glyceride microcapsule powder are dropped in mixer in the mode of equal increments and mixes, form once mixture; Then described once mixture is mixed in the mode of equal increments with PURE WHEY, form secondary mixture; Again described secondary mixture is mixed in the mode of equal increments with soybean isolate protein powder, form three mixtures, in mixed process, control temperature 18 ~ 26 DEG C, humidity≤50%;
3) sieve
By described three mixtures of mixing through 40 order stainless steel mesh, the impurity in raw material, foreign matter are discharged clean;
4) always mix
Again dropped in mixer by raw material after above-mentioned sieving and fully mix, control temperature 18 ~ 26 DEG C, humidity≤50%, finally forms protein powder;
5) detect
Be sub-packed in by protein powder in double-layer plastic bag, sampling detects moisture, passes into ozone in polybag, rocks evenly, tightens the sack of two polybags with band respectively;
6) packing
The qualified protein powder of detection is carried out packing.
preferably,described protein powder is made up of the raw material of following weight portion:
Soybean isolate protein powder 50 parts
PURE WHEY 28 parts
Conjugated linoleic acid glyceride microcapsule powder 20 parts
Powder phospholipid 2 parts.
preferably,described protein powder is made up of the raw material of following weight portion:
Soybean isolate protein powder 88 parts
PURE WHEY 6 parts
Conjugated linoleic acid glyceride microcapsule powder 4 parts
Powder phospholipid 2 parts.
preferably,described protein powder is made up of the raw material of following weight portion:
Soybean isolate protein powder 60 parts
PURE WHEY 25 parts
Conjugated linoleic acid glyceride microcapsule powder 10 parts
Powder phospholipid 5 parts.
another is preferred,described protein powder is made up of the raw material of following weight portion:
Soybean isolate protein powder 60 parts
PURE WHEY 20 parts
Conjugated linoleic acid glyceride microcapsule powder 17 parts
Powder phospholipid 3 parts.
another is preferred,described protein powder is made up of the raw material of following weight portion:
Soybean isolate protein powder 75 parts
PURE WHEY 5 parts
Conjugated linoleic acid glyceride microcapsule powder 14 parts
Powder phospholipid 6 parts.
In above-mentioned formula, soybean isolate protein powder is good protein unique in plant derived protein, and it is rich in high-quality protein needed for human brain and 8 kinds of essential amino acids, contributes to strengthening cerebrovascular function, there is the cholesterol reduced in blood, improve effect of Bone mineral content and bone density;
PURE WHEY comes from milk, is current internationally recognized high-quality animal protein, rich in nutrition content, and lactose content is low, absorbs fast, can provide a large amount of essential amino acid, be conducive to the growth of muscle and the increase of strength after hydrolysis;
Powdered phospholipid is extracted from soybean, and it has the effects such as develop immunitypty, alleviating physical fatigue, reducing blood lipid, adjustment blood sugar, is described as " the 3rd nutrient " arranged side by side with protein, vitamin;
Conjugated linoleic acid glyceride is the stable derivatives of CLA, and metabolism is CLA in vivo, has reduction cholesterol, antiatherosclerosis, raising immunity, improves skeleton density, improves the effect such as nutrient distribution, loss of weight; Oil substances all containing unsoundness in conjugated linoleic acid glyceride microcapsule powder and powdered phospholipid, emulsifying agent can be played in food, the granule of soybean isolate protein powder and PURE WHEY is helped better to disperse, protein powder is more easily reconstituted, and play stable protein powder reconstitute after the effect of drink state, also there is smooth fatty texture and cream fragrance.
beneficial effect of the present invention:
The invention provides a kind of functional protein powder containing conjugated linoleic acid glyceride, its formula comprises 50 ~ 88 parts of soybean isolate protein powders, 5 ~ 30 parts of PURE WHEYs, 3 ~ 20 parts of conjugated linoleic acid glyceride microcapsule powders, 1 ~ 10 part of powdered phospholipid.Formula of the present invention with the addition of this composition of conjugated linoleic acid glyceride on the basis of existing technology, its have improve nutrient distribution, reduce cholesterol, antiatherosclerosis, contribute to the effect reducing blood fat, and there is the effect improving immunity, control body weight, this day by day improves for along with living standard, and the crowd occurring overnutrition or there is overnutrition risk provides a kind of functional food that can prevent and improve rich man's disease; All containing the oil substances of unsoundness in powdered phospholipid in the present invention's formula and conjugated linoleic acid glyceride microcapsule powder, emulsifying agent can be played in food, the granule of soybean isolate protein powder and PURE WHEY can be helped better to disperse, and play stable protein powder reconstitute after the effect of drink state, thus improve dispersiveness and the mouthfeel of the powdery product made, make mouthfeel more smooth, mellow and full, fine and smooth, this protein powder more easily reconstitutes, and is convenient for people to eat.
Detailed description of the invention
Below in conjunction with embodiment, the present invention is described in further detail, but embodiments of the present invention are not limited thereto.
embodiment 1
A functional protein powder containing conjugated linoleic acid glyceride, is made up of the raw material of following weight portion:
| Raw material | Content (%) |
| Soybean isolate protein powder | 50 |
| PURE WHEY | 28 |
| Conjugated linoleic acid glyceride microcapsule powder | 20 |
| Powdered phospholipid | 2 |
Wherein, powder phospholipid is the powder mixture be mixed by 30% lecithin, 24% cephalin and 16% cuorin;
Conjugated linoleic acid glyceride microcapsule powder is purchased from German Kening Co., Ltd (Cognis), and model is Tonalin 60 WDP;
The preparation method of protein powder:
1) weigh:
Each raw material is taken respectively according to above-mentioned formula ratio;
2) slightly mix
Powdered phospholipid and conjugated linoleic acid glyceride microcapsule powder are dropped in mixer in the mode of equal increments and mixes, form once mixture; Then described once mixture is mixed in the mode of equal increments with PURE WHEY, form secondary mixture; Again described secondary mixture is mixed in the mode of equal increments with soybean isolate protein powder, form three mixtures, in mixed process, control temperature 18 ~ 26 DEG C, humidity≤50%;
3) sieve
By three mixtures mixing through 40 order stainless steel mesh, the impurity in raw material, foreign matter are discharged clean;
4) always mix
Again dropped in mixer by raw material after above-mentioned sieving and fully mix, control temperature 18 ~ 26 DEG C, humidity≤50%, finally forms protein powder;
5) detect
Be sub-packed in by protein powder in double-layer plastic bag, sampling detects moisture, passes into ozone in polybag, rocks evenly, tightens the sack of two polybags with band respectively;
6) packing
The qualified protein powder of detection is carried out packing, the minimum package amount of protein powder finished product is 10g ± 0.30g/ bag, specifically carry out suitable selection and adjustment according to the designing requirement of product and the condition of production, must attractive in appearance, the good airproof performance of protein powder minimum package press seal.
embodiment 2
The main technical schemes of the present embodiment and the identical of embodiment 1, difference is:
Functional protein powder containing conjugated linoleic acid glyceride, is made up of the raw material of following weight portion:
| Raw material | Content (%) |
| Soybean isolate protein powder | 88 |
| PURE WHEY | 6 |
| Conjugated linoleic acid glyceride microcapsule powder | 4 |
| Powdered phospholipid | 2 |
embodiment 3
The main technical schemes of the present embodiment and the identical of embodiment 1, difference is:
Functional protein powder containing conjugated linoleic acid glyceride, is made up of the raw material of following weight portion:
| Raw material | Content (%) |
| Soybean isolate protein powder | 60 |
| PURE WHEY | 25 |
| Conjugated linoleic acid glyceride microcapsule powder | 10 |
| Powdered phospholipid | 5 |
embodiment 4
The main technical schemes of the present embodiment and the identical of embodiment 1, difference is:
Functional protein powder containing conjugated linoleic acid glyceride, is made up of the raw material of following weight portion:
| Raw material | Content (%) |
| Soybean isolate protein powder | 60 |
| PURE WHEY | 20 |
| Conjugated linoleic acid glyceride microcapsule powder | 17 |
| Powdered phospholipid | 3 |
embodiment 5
The main technical schemes of the present embodiment and the identical of embodiment 1, difference is:
Functional protein powder containing conjugated linoleic acid glyceride, is made up of the raw material of following weight portion:
| Raw material | Content (%) |
| Soybean isolate protein powder | 75 |
| PURE WHEY | 5 |
| Conjugated linoleic acid glyceride microcapsule powder | 14 |
| Powdered phospholipid | 6 |
protein powder prepared by embodiment 1 to 5 is carried out following Experiment of Zoology respectively:
(1) immunity function test and appraisal zoopery is improved
1, animal used as test NIH mouse 20-22g, single sex, SPF level, often organizes 40, totally four groups.
2, dosage grouping and given the test agent give the time
Sample human body recommended intake is everyone every day of 10g, and everyone presses 60kg batheroom scale, is equivalent to 167mg/kgbw every day.Given the test agent three dosage groups and a negative control group are established in experiment, and be one of them dosage group (middle dosage) with 10 of human body recommended amounts times, i.e. 5 times (low dosage), 30 times (high dose) of two dosage groups, if negative control group.Given the test agent deionized water is prepared, and given the test agent gives 35 days time.Negative control group is deionized water.
3, test method
After animal is quarantined one week in laboratory conditions, each experiment is and at random 40 mouse is divided into four groups below, and often organize 10, every day, gavage gave tested material, and to sample amount weekly according to body weight increase and decrease adjustment, experimental period is 4 weeks.
3..1 the mouse spleen lymphocyte transformation experiment (mtt assay) of ConA induction
Prepared by splenocyte suspension: asepticly get spleen, is placed in and fills appropriate aseptic Hank's liquid plate, ground by spleen gently, make individual cells suspension with tweezers.Filter through 200 eye mesh screens, or with 4 layers of gauze, spleen is ground, wash 2 times with Hank's liquid, each centrifugal 10min(1000r/min).Abandon supernatant cytoplasm is upspring, add 0.5mL aqua sterilisa 20 seconds, 0.5mL2 times of Hank ' s liquid and 8mLHank ' s liquid is added again after splitting erythrocyte, 1000r/min, 10min is centrifugal, then be suspended in the complete culture solution of 1mL by cell, with counting (viable count should more than 95%) after 1% glacial acetic acid dilution, adjustment cell concentration is 3 ~ 5 × 106/mL.
Lymphproliferation response: add in 24 well culture plates by every a splenocyte suspension point holes, every hole 1mL, a hole adds 75 μ L ConA liquid (being equivalent to 7.5 μ g/mL), and 5%CO2 in contrast, is put in another hole, cultivates 72h in 37 DEG C of CO2 incubators.Cultivation terminates front 4h, and every hole sucks supernatant 0.7mL gently, adds 0.7mL not containing the RPMI1640 nutrient solution of calf serum, adds MTT(5mg/mL simultaneously) 50 μ L/holes, continue to cultivate 4h.After cultivation terminates, every hole adds 1mL acid isopropyl alcohol, and piping and druming mixing, makes purple crystal dissolve completely.Then be dispensed in 96 well culture plates, 3 parallel holes are made in each hole, with ELIASA, measure OD value with 570nm wavelength.Also lysate directly can be moved in 2mL cuvette, 721 spectrophotometers measure OD value at wavelength 570nm.
3.2, mouse delayed allergy (the sufficient sole of the foot thickens method)
The 4th day immune animal before off-test and serum are separated, by the sheep red blood cell (SRBC) lumbar injection 0.2ml sensitized animal of 2%, left back sufficient sole of the foot portion thickness is measured after 5 days, then in this place's hypodermic injection 20%(v/v) 24h measures left back sufficient sole of the foot thickness three times after sheep red blood cell (SRBC) (20ul/ only) injection, obtains mean value.
3.3, the mensuration of mice serum hemolysin
After last administration, dilute to obtain india ink with 1:4, inject the india ink (10ml/kg) of dilution by body weight from mouse tail vein, to inject after prepared Chinese ink 2,10min, get blood 20 μ L from angular vein clump respectively, existing side by side is added in 2mL 0.1%Na2CO3 solution by it.With 721 spectrophotometers at 600nm wavelength place densitometric value (OD), make blank with Na2CO3 solution.
3.4, NK cytoactive detection:
Splenocyte suspension must be prepared (effector cell): asepticly get spleen, is placed in the little plate filling appropriate aseptic Hank ' s liquid, is ground by spleen gently, make single cell suspension with tweezers.Net filtration is shone through 200 orders.Hank ' s liquid washes 3 times, each centrifugal 10min(1000r/min).Then be suspended in by cell in the cultivation completely of 2ml, with the blue dyeing counting viable count of platform phenol (should more than 95%), finally adjusting cell concentration with RPMI 1640 complete culture solution be 0.5 × 107/ml.
NK cytoactive detects: get target cell that concentration is 1 х, 105/ml and each 100ul(of effector cell imitates target than 50:1), add in U-shaped 96 well culture plates; Target cell Spontaneous release hole adds target cell and each 100ul of nutrient solution, and the maximum release aperture of target cell adds target cell and each 100ul of 1%NP40 or 2.5%Triton; Above-mentionedly everyly all establish three parallel holes, in 37 DEG C, 4h is cultivated in 5%CO2 incubator, then by 96 well culture plates with the centrifugal 5min of 1500r/min, every hole is drawn in 96 well culture plates at the bottom of supernatant 100ul horizontalization, adds LDH matrix liquid 100ul, according to room temperature differential responses 3 ~ 10min simultaneously, every hole adds the HCl 30ul of 1mol/L, measures OD value (OD) at ELIASA 490nm place.
Data processing: all data all carry out variance analysis through SPSS statistical software.
4, experimental result
4.1 even in everyday situations are observed and body weight: each administration group mouse all normally increases without no abnormality seens such as significant difference (P>0.05), the state of mind in experimental session body weight.
The 4.2 mouse spleen lymphocyte conversion reactions of induce ConA containing the functional protein powder of conjugated linoleic acid glyceride, Splenic vein hemodynamics reaction are the impact (see table 1) of negative findings, mice serum hemolytic antibody drop reaction, NK cytoactive: the functional protein powder containing conjugated linoleic acid glyceride is negative findings on the impact that Splenic vein hemodynamics reacts; On the impact of mouse delayed allergy, in test rent, the recipient site thickness of each dosage group mouse obviously increases, compare with control group through statistical procedures, difference has pole significant, shows that the affect result of the test of the functional protein powder containing conjugated linoleic acid glyceride on mouse delayed allergy is positive; On in the impact of mice serum hemolytic antibody drop reaction, the antibody volume of the middle dosage group of test group is higher than control group, through statistical procedures, meaning that there was a significant difference, shows that the functional protein powder containing conjugated linoleic acid glyceride affects result of the test for positive to mice serum hemolytic antibody drop reaction; On the impact of NK cytoactive, the NK cytoactive of the low dose group of test group is higher than control group, meaning that there was a significant difference, the NK cytoactive of high, middle dosage higher than control group, significant difference meaning has pole significant, shows that the functional protein powder containing conjugated linoleic acid glyceride affects result of the test for positive to NK cytoactive.
The functional protein powder develop immunitypty test impact of table 1. containing conjugated linoleic acid glyceride (
)
5, conclusion
Functional protein powder containing conjugated linoleic acid glyceride can improve the mouse delayed allergy ability (the sufficient sole of the foot thickens method) of sheep red blood cell (SRBC) induction; Animal subject Hemolysin titre is reacted increase, increase the NK cytoactive of animal subject.Therefore, result shows that the functional protein containing conjugated linoleic acid glyceride has the health-care efficacy of develop immunitypty.
(2) auxiliary lipid-lowering function test and appraisal zoopery
1, experimental principle
Disorders of lipid metabolism animal model can be formed with high cholesterol and lipid forage feed animal, give animal given the test agent again or give given the test agent simultaneously, the impact of given the test agent on hyperlipidemia can be detected, and the impact that given the test agent produces the absorption of lipid, the formation of lipoprotein, the degraded of lipid or excretion can be judged.
2, experimental technique
2.1 animals used as test: healthy adult Wistar kind male rat (150-200g), SPF level, often organizes 10.
2.2 high lipid foods: 78.8% basal feed, 1% cholesterol, 10% yolk powder and 10% lard, 0.2% cholate
2.3 dosage groupings and given the test agent give the time
Sample human body recommended intake is everyone every day of 10g, and everyone presses 60kg batheroom scale, is equivalent to 167mg/kgbw every day.Three dosage groups and a high fat control group are established in experiment, with 5 of human body recommended amounts times for low dose group, separately establish 10 times, 30 times two dosage groups, are respectively middle and high dosage group.Given the test agent deionized water is prepared, and gives sample 35d continuously.Regularly weigh in, terminate fasting 16h with experiment, get the every blood lipids index of tail hematometry.
2.4 key instruments and reagent: the kit of animal balance, electronic balance, low speed centrifuge, 722 type spectrophotometers, thermostat water bath, micro sample adding appliance, operating theater instruments, mensuration TC, TG, HDL-C provides by Zhongsheng Beikong Biological Science & Technology Co., Ltd..
2.5 experimental data statistics: all data all carry out variance analysis through SPSS statistical software, and variance uses variance analysis together, heterogeneity of variance rank test.
3, experimental result
3.1 contain the functional protein powder of conjugated linoleic acid glyceride to the impact of rat body weight: as shown in table 2, the body weight of given the test agent each dosage treated animal is more proud with high fat control group, without significant difference (P>0.05).
The functional protein powder of table 2. containing conjugated linoleic acid glyceride on the impact of rat body weight (
)
| Group | Number of animals (only) | Initial stage body weight | Latter stage body weight |
| High fat control group | 10 | 166.25±5.45 | 328.10±20.12 |
| Low dose group | 10 | 169.36±7.34 | 324.35±18.99 |
| Middle dosage group | 10 | 167.52±6.88 | 322.49±24.16 |
| High dose group | 10 | 168.35±8.46 | 325.43±19.76 |
3.2 contain the functional protein powder of conjugated linoleic acid glyceride to the impact of rat fat content
3.2.1 rat fat contents level (as shown in table 3) before experiment
Front rat fat contents level tested by table 3.
| Group | Number of animals (only) | TC（mmol/L） | TG（mmol/L） | HDL-C（mmol/L） |
| High fat control group | 10 | 0.85±0.20 | 0.80±0.22 | 1.25±0.30 |
| Low dose group | 10 | 0.86±0.17 | 0.79±0.27 | 1.28±0.39 |
| Middle dosage group | 10 | 0.83±0.24 | 0.82±0.36 | 1.22±0.41 |
| High dose group | 10 | 0.87±0.22 | 0.78±0.30 | 1.25±0.37 |
3.2.2 rat fat contents level (as shown in table 4) after experiment
Rear rat fat contents level tested by table 4.
| Group | Number of animals (only) | TC mmol/L） | TG（（mmol/L） | HDL-C（mmol/L） |
| High fat control group | 10 | 2.24±0.36 | 2.70±0.68 | 1.75±0.52 |
| Low dose group | 10 | 2.20±0.35 | 2.61±0.53 | 1.80±0.68 |
| Middle dosage group | 10 | 1.61±0.38* | 1.90±0.44* | 1.82±0.71 |
| High dose group | 10 | 1.57±0.40* | 1.87±0.54* | 1.88±0.40 |
Compare with high fat of blood control group: *
P<0.05.
4, conclusion
This experiment shows, per os give rat various dose containing the functional protein powder sample of conjugated linoleic acid glyceride, compared with high fat control group, the middle and high dosage group of sample can significantly reduce High fat diet rats TC, TG level (
p<0.05).Therefore judge according to above experimental result, the functional protein powder containing conjugated linoleic acid glyceride has auxiliary lipid-lowering function, can improve nutrient distribution in human body, thus has the health-care efficacy of prevention rich man's disease.
Finally should be noted that; above embodiment is only in order to illustrate technical scheme of the present invention; but not limiting the scope of the invention; although done to explain to the present invention with reference to preferred embodiment; those of ordinary skill in the art is to be understood that; can modify to technical scheme of the present invention or equivalent replacement, and not depart from essence and the scope of technical solution of the present invention.
Claims (6)
1. the functional protein powder containing conjugated linoleic acid glyceride, is characterized in that: described protein powder is made up of the raw material of following weight portion:
Soybean isolate protein powder 50 ~ 88 parts
PURE WHEY 5 ~ 30 parts
Conjugated linoleic acid glyceride microcapsule powder 3 ~ 20 parts
Powder phospholipid 1 ~ 10 part;
Wherein, described powder phospholipid is the powder mixture be mixed by 30% lecithin, 24% cephalin and 16% cuorin;
Described conjugated linoleic acid glyceride microcapsule powder is provided by German Kening Co., Ltd, and its model is Tonalin 60 WDP;
The preparation method of described protein powder:
1) weigh:
Each raw material is taken respectively according to above-mentioned formula ratio;
2) slightly mix
Powdered phospholipid and conjugated linoleic acid glyceride microcapsule powder are dropped in mixer in the mode of equal increments and mixes, form once mixture; Then described once mixture is mixed in the mode of equal increments with PURE WHEY, form secondary mixture; Again described secondary mixture is mixed in the mode of equal increments with soybean isolate protein powder, form three mixtures, in mixed process, control temperature 18 ~ 26 DEG C, humidity≤50%;
3) sieve
By described three mixtures of mixing through 40 order stainless steel mesh, the impurity in raw material, foreign matter are discharged clean;
4) always mix
Again dropped in mixer by raw material after above-mentioned sieving and fully mix, control temperature 18 ~ 26 DEG C, humidity≤50%, finally forms protein powder;
5) detect
Be sub-packed in by protein powder in double-layer plastic bag, sampling detects moisture, passes into ozone in polybag, rocks evenly, tightens the sack of two polybags with band respectively;
6) packing
The qualified protein powder of detection is carried out packing.
2. a kind of functional protein powder containing conjugated linoleic acid glyceride according to claim 1, is characterized in that: described protein powder is made up of the raw material of following weight portion:
Soybean isolate protein powder 50 parts
PURE WHEY 28 parts
Conjugated linoleic acid glyceride microcapsule powder 20 parts
Powder phospholipid 2 parts.
3. a kind of functional protein powder containing conjugated linoleic acid glyceride according to claim 1, is characterized in that: described protein powder is made up of the raw material of following weight portion:
Soybean isolate protein powder 88 parts
PURE WHEY 6 parts
Conjugated linoleic acid glyceride microcapsule powder 4 parts
Powder phospholipid 2 parts.
4. a kind of functional protein powder containing conjugated linoleic acid glyceride according to claim 1, is characterized in that: described protein powder is made up of the raw material of following weight portion:
Soybean isolate protein powder 60 parts
PURE WHEY 25 parts
Conjugated linoleic acid glyceride microcapsule powder 10 parts
Powder phospholipid 5 parts.
5. a kind of functional protein powder containing conjugated linoleic acid glyceride according to claim 1, is characterized in that: described protein powder is made up of the raw material of following weight portion:
Soybean isolate protein powder 60 parts
PURE WHEY 20 parts
Conjugated linoleic acid glyceride microcapsule powder 17 parts
Powder phospholipid 3 parts.
6. a kind of functional protein powder containing conjugated linoleic acid glyceride according to claim 1, is characterized in that: described protein powder is made up of the raw material of following weight portion:
Soybean isolate protein powder 75 parts
PURE WHEY 5 parts
Conjugated linoleic acid glyceride microcapsule powder 14 parts
Powder phospholipid 6 parts.
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| CN105533713A (en) * | 2015-12-29 | 2016-05-04 | 广州市戴菊科技发展有限公司 | Medical food with anti-ageing function and preparation method of medical food |
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| CN108703375A (en) * | 2018-04-04 | 2018-10-26 | 大连医诺生物股份有限公司 | Weight losing function food containing conjugated linoleic acid |
| CN108741029B (en) * | 2018-05-28 | 2021-11-09 | 广州正广生物科技有限公司 | Ketogenic dietary nutrition powder and preparation method thereof |
| CN112889987A (en) * | 2019-11-19 | 2021-06-04 | 内蒙古蒙牛乳业（集团）股份有限公司 | Food composition, tablet candy and application |
| CN111713696A (en) * | 2020-07-21 | 2020-09-29 | 本元大健康科技产业(广东)有限公司 | Weight management meal replacement food and preparation method thereof |
| CN113424962A (en) * | 2020-09-21 | 2021-09-24 | 黑龙江飞鹤乳业有限公司 | Nutrient component for improving immunity, food or health-care product containing nutrient component and application of nutrient component |
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