CN103190630A - Functional protein powder containing conjugated linoleic acid glycerides - Google Patents
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Abstract
The invention relates to the technical field of functional food, in particular to a functional protein powder containing conjugated linoleic acid glycerides. The formula of the functional protein powder comprises 50 to 88 parts of soybean isolated protein powder, 5 to 30 parts of whey protein powder, 3 to 20 parts of conjugated linoleic acid glyceride microcapsule powder and 1 to 10 parts of powder phospholipid. The conjugated linoleic acid glyceride composition added in the formula not only can be used for reducing cholesterol and resisting the atherosis so as to be favorable for reducing the blood fat, but also has a function for improving the immunity, improving the nutritive allocation and controlling the weight. A functional food with multiple healthcare functions for preventing and improving affluence illnesses can be provided for people with overnutrition or having the risk of overnutrition along the rising living standard.
Description
Technical field
The present invention relates to the functional food technical field, be specifically related to a kind of functional protein powder that contains conjugated linoleic acid glyceride.
Background technology
Modern society's people's rhythm of life is more and more faster, operating pressure, diet are unbalanced etc., and various factors causes human body in sub-health state, particularly due to the long-term food that edible oil and fat content is high, heat is high, amount of exercise reduces, and causes the various symptoms such as overnutrition, as rich man's disease.Rich man's disease has become the main diseases kind of harm compatriots health at present.According to statistics, China the elderly 15～20% constipation, the sixty percentth, white collar; According to Ministry of Public Health investigation, in state-owned 22% people overweight, more than 6,000 ten thousand people seek medical advice because of fat, hypertension is the people more than 200,000,000, diabetes more than 5,000 ten thousand people, high fat of blood 1.6 hundred million people.The whole nation surpasses 1.5 ten thousand because " rich man's disease " causes death toll every day, account for more than 70% of total toll, the expense for the treatment of due to rich man's disease accounts for more than 60% of disease burden, comprise that the western countries medical personnels such as the U.S., Britain also repeatedly deliver survey report, the development of China's rich man's disease that affluence is got up feels concern, and estimates that rich man's disease in 2015 more is widely current in China.Thereby the meaning of prevention this overnutrition of rich man's disease or nutritious superfluous risk is equally important with extra-nutrition, become the focus of research.
The nutrition regulation teacher that protein powder comes from American-European countries the earliest strengthens the research of the aspects such as locomitivity, fatigue-relieving to the sportsman, to be born according to the movement characteristic of human body cell, tissue, muscle, main component is protein, protein to building up health, improve immunity, keep full of vitality and all play an important role.Because its effect is remarkable, instant, get most of the attention, and is being once that many national sports team must not disclosed wuwa.Along with the reach of science, nutritionists update protein powder, produce gradually applicable crowd's scope novel nourishing food more widely, and it is extended to common people house from sports team.The primary raw material of current protein powder on the market is mostly soybean isolate protein powder and/or PURE WHEY, be equipped with phosphatide, polysaccharide, mineral matter, vitamin, soya-bean polypeptides, or add the essence such as chocolate flavoured, fruity, vanillic aldehyde on a small quantity, or add the material such as sweetener, for example: Amway Nutrilite protein powder, its primary raw material is soybean protein isolate, concentrated milk protein, lecithin, silica; The soup minister is strong protein powder doubly, its primary raw material: soybean protein isolate, lactalbumin, soybean lecithin, these protein powders of vegetable fat powder; Without the thresold protein powder, soybean protein isolate, malt flour, soy peptide powder, maltodextrin, silica, wheat face powder end essence, acesulfame potassium etc., the formula of above-mentioned range protein powder is relatively simple, its effect is mainly to supplement the nutritional labeling such as albumen to improve physique, fatigue-relieving, enhancing physical efficiency etc., health-care efficacy is relatively single, the protein powder of the overnutrition particularly do not occurred for modern society, the prevention effect of this rich man's disease of Hyperlipemia.
Scientific research is found, CLA is a kind of natural mixed active material be present in organism, safe, nontoxic, there is the immunity of raising, reduce the effects such as cholesterol, antiatherosclerosis, raising skeleton density, loss of weight, can be used for the health food of preparation prevention rich man's disease.As Chinese invention patent (CN101731335A) discloses a kind of CLA Yoghourt and preparation method of being rich in, Chinese invention patent (CN1686532A) discloses a kind of people to lose weight nutrient that contains CLA.Yet CLA is unstable, as be processed into Powdered and add in food complicated process of preparation to, and there is certain astringent taste and smell, mouthfeel poor being unfavorable for eats, thereby need badly and a kind ofly can substitute CLA and there is nutrient distribution, auxiliary antilipemic in the human body of improvement, can prevent rich man's disease to there is building up health simultaneously, improve the multiple efficacies such as immunity, and be convenient for people to reconstitute edible functional protein powder.But at home and abroad there is no at present the relevant report of the protein powder about containing conjugated linoleic acid glyceride.
Summary of the invention
The object of the invention is to avoid weak point of the prior art and a kind of functional protein powder that contains conjugated linoleic acid glyceride is provided.
Purpose of the present invention is achieved through the following technical solutions:
A kind of functional protein powder that contains conjugated linoleic acid glyceride is provided, and described protein powder is comprised of the raw material of following weight portion:
50～88 parts of soybean isolate protein powders
5～30 parts of PURE WHEYs
3～20 parts of conjugated linoleic acid glyceride microcapsule powders
1～10 part of powder phospholipid;
Wherein, described powder phospholipid is the powder mixture be mixed by 30% lecithin, 24% cephalin and 16% cuorin;
The preparation method of described protein powder:
1) weighing:
Take respectively each raw material according to above-mentioned formula ratio;
2) thick mixed
The mode that powdered phospholipid is increased progressively with equivalent with the conjugated linoleic acid glyceride microcapsule powder drops in mixer is mixed, and forms once mixture; Then the mode described once mixture increased progressively with equivalent with PURE WHEY is mixed, and forms secondary mixture; The mode more described secondary mixture increased progressively with equivalent with soybean isolate protein powder mixes, and forms mixture three times, in mixed process, controls 18～26 ℃ of temperature, humidity≤50%;
3) sieve
By described three mixtures of mixing, through 40 order stainless steel mesh, by the impurity in raw material, foreign matter, discharge clean;
4) total mixed
Raw material after above-mentioned sieving is again dropped in mixer and fully mixes, control 18～26 ℃ of temperature, humidity≤50%, finally form protein powder;
5) detect
Protein powder is sub-packed in double-layer plastic bag, and sampling detects moisture, passes into ozone in polybag, rocks evenly, tightens respectively the sack of two polybags with band;
6) packing
Carry out packing by detecting qualified protein powder.
preferably,described protein powder is comprised of the raw material of following weight portion:
50 parts of soybean isolate protein powders
28 parts of PURE WHEYs
20 parts of conjugated linoleic acid glyceride microcapsule powders
2 parts of powder phospholipids.
preferably,described protein powder is comprised of the raw material of following weight portion:
88 parts of soybean isolate protein powders
6 parts of PURE WHEYs
4 parts of conjugated linoleic acid glyceride microcapsule powders
2 parts of powder phospholipids.
preferably,described protein powder is comprised of the raw material of following weight portion:
60 parts of soybean isolate protein powders
25 parts of PURE WHEYs
10 parts of conjugated linoleic acid glyceride microcapsule powders
5 parts of powder phospholipids.
another is preferred,described protein powder is comprised of the raw material of following weight portion:
60 parts of soybean isolate protein powders
20 parts of PURE WHEYs
17 parts of conjugated linoleic acid glyceride microcapsule powders
3 parts of powder phospholipids.
another is preferred,described protein powder is comprised of the raw material of following weight portion:
75 parts of soybean isolate protein powders
5 parts of PURE WHEYs
14 parts of conjugated linoleic acid glyceride microcapsule powders
6 parts of powder phospholipids.
In above-mentioned formula, soybean isolate protein powder is good protein unique in plant origin protein, and it is rich in the required high-quality protein of human brain and 8 kinds of essential amino acids, contributes to strengthen cerebrovascular function, there is the cholesterol reduced in blood, improve the effect of Bone mineral content and bone density;
PURE WHEY comes from milk, is current internationally recognized high-quality animal protein, and nutritional labeling is abundant, and lactose content is low, after hydrolysis, absorbs soon, and a large amount of essential amino acids can be provided, and is conducive to the growth of muscle and the increase of strength;
Powdered phospholipid is extracted from soybean, and it has the effects such as the immunity of enhancing, alleviating physical fatigue, reducing blood lipid, adjustment blood sugar, is described as " the 3rd nutrient " arranged side by side with protein, vitamin;
Conjugated linoleic acid glyceride is the stable derivatives of CLA, and metabolism is CLA in vivo, have reduce cholesterol, antiatherosclerosis, raising immunity, improve skeleton density, improve nutrient distribution, the effect such as loss of weight; All contain the oil substances of unsoundness in conjugated linoleic acid glyceride microcapsule powder and powdered phospholipid, can play emulsifying agent in food, help the granule of soybean isolate protein powder and PURE WHEY better to disperse, protein powder is more easily reconstituted, and the effect of the drink state after playing the stable protein powder and reconstituting, also there is smooth fatty texture and cream fragrance.
beneficial effect of the present invention:
The invention provides a kind of functional protein powder that contains conjugated linoleic acid glyceride, its formula comprises 50～88 parts of soybean isolate protein powders, 5～30 parts of PURE WHEYs, 3～20 parts of conjugated linoleic acid glyceride microcapsule powders, 1～10 part of powdered phospholipid.Formula of the present invention has added this composition of conjugated linoleic acid glyceride on the basis of existing technology, it has the nutrient distribution of improvement, reduces cholesterol, antiatherosclerosis, contribute to reduce the effect of blood fat, and there is the effect that improves immunity, controls body weight, this,, for along with living standard day by day improves, overnutrition occurs or exists the crowd of overnutrition risk that a kind of functional food that can prevent and improve rich man's disease is provided; All contain the oil substances of unsoundness in powdered phospholipid in the present invention's formula and conjugated linoleic acid glyceride microcapsule powder, can play emulsifying agent in food, can help the granule of soybean isolate protein powder and PURE WHEY better to disperse, and the effect of the drink state after playing the stable protein powder and reconstituting, thereby dispersiveness and the mouthfeel of the powdery product of making have been improved, make mouthfeel more smooth, mellow and full, fine and smooth, this protein powder more easily reconstitutes, and is convenient for people to eat.
The specific embodiment
Below in conjunction with embodiment, the present invention is described in further detail, but embodiments of the present invention are not limited to this.
embodiment 1
A kind of functional protein powder that contains conjugated linoleic acid glyceride is comprised of the raw material of following weight portion:
| Raw material | Content (%) |
| Soybean isolate protein powder | 50 |
| PURE WHEY | 28 |
| The conjugated linoleic acid glyceride microcapsule powder | 20 |
| Powdered phospholipid | 2 |
Wherein, powder phospholipid is the powder mixture be mixed by 30% lecithin, 24% cephalin and 16% cuorin;
The conjugated linoleic acid glyceride microcapsule powder is purchased from German Kening Co.,Ltd (Cognis), and model is Tonalin 60 WDP;
The preparation method of protein powder:
1) weighing:
Take respectively each raw material according to above-mentioned formula ratio;
2) thick mixed
The mode that powdered phospholipid is increased progressively with equivalent with the conjugated linoleic acid glyceride microcapsule powder drops in mixer is mixed, and forms once mixture; Then the mode described once mixture increased progressively with equivalent with PURE WHEY is mixed, and forms secondary mixture; The mode more described secondary mixture increased progressively with equivalent with soybean isolate protein powder mixes, and forms mixture three times, in mixed process, controls 18～26 ℃ of temperature, humidity≤50%;
3) sieve
By three mixtures mixing, through 40 order stainless steel mesh, by the impurity in raw material, foreign matter, discharge clean;
4) total mixed
Raw material after above-mentioned sieving is again dropped in mixer and fully mixes, control 18～26 ℃ of temperature, humidity≤50%, finally form protein powder;
5) detect
Protein powder is sub-packed in double-layer plastic bag, and sampling detects moisture, passes into ozone in polybag, rocks evenly, tightens respectively the sack of two polybags with band;
6) packing
Carry out packing by detecting qualified protein powder, the minimum package amount of protein powder finished product is 10g ± 0.30g/ bag, specifically according to designing requirement and the condition of production of product, carry out suitable selection and adjustment, must protein powder minimum package press seal attractive in appearance, good airproof performance.
embodiment 2
The main technical schemes of the present embodiment is identical with embodiment's 1, and difference is:
The functional protein powder that contains conjugated linoleic acid glyceride is comprised of the raw material of following weight portion:
| Raw material | Content (%) |
| Soybean isolate protein powder | 88 |
| PURE WHEY | 6 |
| The conjugated linoleic acid glyceride microcapsule powder | 4 |
| Powdered phospholipid | 2 |
embodiment 3
The main technical schemes of the present embodiment is identical with embodiment's 1, and difference is:
The functional protein powder that contains conjugated linoleic acid glyceride is comprised of the raw material of following weight portion:
| Raw material | Content (%) |
| Soybean isolate protein powder | 60 |
| PURE WHEY | 25 |
| The conjugated linoleic acid glyceride microcapsule powder | 10 |
| Powdered phospholipid | 5 |
embodiment 4
The main technical schemes of the present embodiment is identical with embodiment's 1, and difference is:
The functional protein powder that contains conjugated linoleic acid glyceride is comprised of the raw material of following weight portion:
| Raw material | Content (%) |
| Soybean isolate protein powder | 60 |
| PURE WHEY | 20 |
| The conjugated linoleic acid glyceride microcapsule powder | 17 |
| Powdered phospholipid | 3 |
embodiment 5
The main technical schemes of the present embodiment is identical with embodiment's 1, and difference is:
The functional protein powder that contains conjugated linoleic acid glyceride is comprised of the raw material of following weight portion:
| Raw material | Content (%) |
| Soybean isolate protein powder | 75 |
| PURE WHEY | 5 |
| The conjugated linoleic acid glyceride microcapsule powder | 14 |
| Powdered phospholipid | 6 |
the protein powder of embodiment 1 to 5 preparation is carried out respectively to following Experiment of Zoology:
(1) improve immunity function test and appraisal zoopery
1, animal used as test NIH mouse 20-22g, single sex, SPF level, 40 every group, totally four groups.
2, dosage grouping and given the test agent give the time
Sample human body recommended intake is everyone 10g every day, and everyone presses the 60kg batheroom scale, is equivalent to 167mg/kgbw every day.Three dosage groups of given the test agent and a negative control group are established in experiment, and 10 times of human body recommended amounts of take be one of them dosage group (middle dosage), and two dosage groups are 5 times (low dosage), 30 times (high dose), establish negative control group.Given the test agent is prepared by deionized water, and given the test agent gives 35 days time.Negative control group is deionized water.
3, test method
After animal is quarantined one week under laboratory condition, below each experiment be at random 40 mouse be divided into to four groups, 10 every group, every day, gavage gave tested material, to the sample amount weekly according to body weight increase and decrease adjust, experimental period is 4 weeks.
3..1 the mouse spleen lymphocyte transformation experiment (mtt assay) that ConA induces
The splenocyte suspension preparation: the aseptic spleen of getting, be placed in and fill appropriate aseptic Hank's liquid plate, with tweezers, gently spleen is ground, make the individual cells suspension.Filter through 200 eye mesh screens, or with 4 layers of gauze, spleen is ground, wash 2 times each centrifugal 10min(1000r/min with Hank's liquid).Abandoning supernatant upsprings cytoplasm, add the 0.5mL aqua sterilisa 20 seconds, add again 0.5mL2 times of Hank ' s liquid and 8mLHank ' s liquid after splitting erythrocyte, 1000r/min, 10min is centrifugal, then cell is suspended in the complete culture solution of 1mL, with counting after 1% glacial acetic acid dilution (viable count should more than 95%), adjusting cell concentration be 3～5 * 106/mL.
Lymphproliferation response: divide two holes to add in 24 well culture plates every a splenocyte suspension, 1mL，Yi hole, every hole adds 75 μ L ConA liquid (being equivalent to 7.5 μ g/mL), and 5%CO2 in contrast, is put in another hole, in 37 ℃ of CO2 incubators, cultivates 72h.Cultivate and finish front 4h, every hole sucks supernatant 0.7mL gently, adds 0.7mL containing the RPMI1640 nutrient solution of calf serum, adds MTT(5mg/mL simultaneously) 50 μ L/holes, continue to cultivate 4h.After cultivating end, every hole adds 1mL acid isopropyl alcohol, and piping and druming mixes, and purple crystal is dissolved fully.Then divide and install in 96 well culture plates, 3 parallel holes are made in each hole, use ELIASA, with the 570nm wavelength, measure OD value.Also lysate directly can be moved in the 2mL cuvette, on 721 spectrophotometers, at wavelength 570nm, measure the OD value.
3.2, mouse delayed allergy (the sufficient sole of the foot thickens method)
The 4th day immune animal and serum before off-test separate, sheep red blood cell (SRBC) lumbar injection 0.2ml sensitized animal with 2%, measure left back sufficient sole of the foot section thickness after 5 days, then in this place's hypodermic injection 20%(v/v) 24h measures left back sufficient sole of the foot thickness three times after sheep red blood cell (SRBC) (20ul/ is only) injection, obtains mean value.
3.3, the mensuration of mice serum hemolysin
After the last administration, dilute to obtain india ink with 1:4, inject the india ink (10ml/kg) of dilution by body weight from mouse tail vein, inject after prepared Chinese ink 2,10min, get blood 20 μ L from the angular vein clump respectively, existing side by side, soon it is added in 2mL 0.1%Na2CO3 solution.At 600nm wavelength place's photometry density value (OD), with Na2CO3 solution, make blank with 721 spectrophotometers.
3.4, the NK cytoactive detection:
Splenocyte suspension must prepare (effector cell): the aseptic spleen of getting, be placed in the little plate that fills appropriate aseptic Hank ' s liquid, and with tweezers, gently spleen is ground, make single cell suspension.Shine net filtration through 200 orders.Hank ' s liquid is washed 3 times, each centrifugal 10min(1000r/min).Then cell being suspended in the cultivation fully of 2ml, with the blue dyeing counting viable count of platform phenol (should more than 95%), be finally 0.5 * 107/ml with RPMI 1640 complete culture solutions adjustment cell concentrations.
The NK cytoactive detects: get concentration and be the target cell of 105/ml of 1 х and each 100ul(effect target of effector cell than 50:1), add in U-shaped 96 well culture plates; Target cell Spontaneous release hole adds target cell and each 100ul of nutrient solution, and the maximum release aperture of target cell adds target cell and 1%NP40 or each 100ul of 2.5%Triton; Above-mentioned every three parallel holes of all establishing, in 37 ℃, cultivate 4h in the 5%CO2 incubator, then by 96 well culture plates with the centrifugal 5min of 1500r/min, in 96 well culture plates, add LDH matrix liquid 100ul, according to room temperature differential responses 3～10min at the bottom of every hole absorption supernatant 100ul horizontalization simultaneously, every hole adds the HCl 30ul of 1mol/L, measures OD value (OD) at ELIASA 490nm place.
Data are processed: all data are all carried out variance analysis through the SPSS statistical software.
4, experimental result
4.1 even in everyday situations is observed and body weight: each administration group mouse at the equal normal growth of experimental session body weight without no abnormality seens such as significant difference (P > 0.05), the state of mind.
4.2 the impact (referring to table 1) of the mouse spleen lymphocyte conversion reaction that the functional protein powder that contains conjugated linoleic acid glyceride is induced ConA, the negative result of SPL conversion reaction, mice serum hemolytic antibody drop reaction, NK cytoactive: the functional protein powder that contains conjugated linoleic acid glyceride is on the negative result of the impact of SPL conversion reaction; Impact on the mouse delayed allergy, in the test rent, the tested position thickness of each dosage group mouse obviously increases, learn more by statistics processing with control group, difference has utmost point significant, the functional protein powder that shows to contain conjugated linoleic acid glyceride on the mouse delayed allergy to affect result of the test positive; In the impact of mice serum hemolytic antibody drop reaction, the antibody volume of the middle dosage group of test group is higher than control group, learn and process by statistics, meaning that there was a significant difference, show that the functional protein powder that contains conjugated linoleic acid glyceride affects result of the test to mice serum hemolytic antibody drop reaction positive; Impact on the NK cytoactive, the NK cytoactive of the low dose group of test group is higher than control group, meaning that there was a significant difference, the NK cytoactive of high, middle dosage higher than control group, the significant difference meaning has utmost point significant, shows that the functional protein powder that contains conjugated linoleic acid glyceride affects result of the test to the NK cytoactive positive.
The functional protein powder enhancing immunity test impact that table 1. contains conjugated linoleic acid glyceride (
)
5, conclusion
The functional protein powder that contains conjugated linoleic acid glyceride can improve the mouse delayed allergy ability (the sufficient sole of the foot thickens method) that sheep red blood cell (SRBC) is induced; The reaction of animal subject Hemolysin titre is increased, increase the NK cytoactive of animal subject.Therefore, result shows that the functional protein that contains conjugated linoleic acid glyceride has the health-care efficacy that strengthens immunity.
(2) auxiliary lipid-lowering function test and appraisal zoopery
1, experimental principle
Can form the disorders of lipid metabolism animal model with high cholesterol and lipid forage feed animal, give again the animal given the test agent or give given the test agent simultaneously, can detect the impact of given the test agent on hyperlipidemia, and can judge that given the test agent is on the absorption of lipid, the formation of lipoprotein, the degraded of lipid or the impact that excretion produces.
2, experimental technique
2.1 animal used as test: healthy adult Wistar kind male rat (150-200g), SPF level, 10 every group.
2.2 high lipid food: 78.8% basal feed, 1% cholesterol, 10% yolk powder and 10% lard, 0.2% cholate
2.3 dosage grouping and given the test agent give the time
Sample human body recommended intake is everyone 10g every day, and everyone presses the 60kg batheroom scale, is equivalent to 167mg/kgbw every day.Three dosage groups and a high fat control group are established in experiment, and 5 times of human body recommended amounts of take are low dose group, separately establish 10 times, 30 times two dosage groups, are respectively middle and high dosage group.Given the test agent is prepared by deionized water, gives continuously sample 35d.Regularly weigh in, with experiment, finish fasting 16h, get the every blood lipids index of tail hematometry.
2.4 key instrument and reagent: the kit of animal balance, electronic balance, low speed centrifuge, 722 type spectrophotometers, thermostat water bath, micro sample adding appliance, operating theater instruments, mensuration TC, TG, HDL-C provides by Zhongsheng Beikong Biological Science & Technology Co., Ltd..
2.5 the experimental data statistics: all data are all carried out variance analysis through the SPSS statistical software, and variance is used variance analysis together, the heterogeneity of variance rank test.
3, experimental result
3.1 the impact of the functional protein powder that contains conjugated linoleic acid glyceride on rat body weight: as shown in table 2, the body weight of each dosage treated animal of given the test agent and high fat control group are more proud, without significant difference (P > 0.05).
The functional protein powder that table 2. contains conjugated linoleic acid glyceride on the impact of rat body weight (
)
| Group | Number of animals (only) | The initial stage body weight | Latter stage body weight |
| High fat control group | 10 | 166.25±5.45 | 328.10±20.12 |
| Low dose group | 10 | 169.36±7.34 | 324.35±18.99 |
| Middle dosage group | 10 | 167.52±6.88 | 322.49±24.16 |
| High dose group | 10 | 168.35±8.46 | 325.43±19.76 |
3.2 the impact of the functional protein powder that contains conjugated linoleic acid glyceride on rat fat content
3.2.1 rat fat contents level (as shown in table 3) before experiment
Rat fat contents level before table 3. experiment
| Group | Number of animals (only) | TC（mmol/L） | TG（mmol/L） | HDL-C（mmol/L） |
| High fat control group | 10 | 0.85±0.20 | 0.80±0.22 | 1.25±0.30 |
| Low dose group | 10 | 0.86±0.17 | 0.79±0.27 | 1.28±0.39 |
| Middle dosage group | 10 | 0.83±0.24 | 0.82±0.36 | 1.22±0.41 |
| High dose group | 10 | 0.87±0.22 | 0.78±0.30 | 1.25±0.37 |
3.2.2 rat fat contents level (as shown in table 4) after experiment
Rat fat contents level after table 4. experiment
| Group | Number of animals (only) | TC mmol/L） | TG（（mmol/L） | HDL-C（mmol/L） |
| High fat control group | 10 | 2.24±0.36 | 2.70±0.68 | 1.75±0.52 |
| Low dose group | 10 | 2.20±0.35 | 2.61±0.53 | 1.80±0.68 |
| Middle dosage group | 10 | 1.61±0.38* | 1.90±0.44* | 1.82±0.71 |
| High dose group | 10 | 1.57±0.40* | 1.87±0.54* | 1.88±0.40 |
With the high fat of blood control group, compare: *
P<0.05.
4, conclusion
This experiment shows, per os gives the functional protein powder sample that contains conjugated linoleic acid glyceride of rat various dose, with high fat control group, compare, the middle and high dosage group of sample can significantly reduce high fat rat TC, TG level (
p<0.05).Therefore judge according to above experimental result, the functional protein powder that contains conjugated linoleic acid glyceride has auxiliary lipid-lowering function, can improve nutrient distribution in human body, thereby there is the health-care efficacy of prevention rich man's disease.
Finally should be noted that; above embodiment is only in order to illustrate technical scheme of the present invention; but not limiting the scope of the invention; although with reference to preferred embodiment, the present invention has been done to explain; those of ordinary skill in the art is to be understood that; can modify or be equal to replacement technical scheme of the present invention, and not break away from essence and the scope of technical solution of the present invention.
Claims (6)
1. a functional protein powder that contains conjugated linoleic acid glyceride, it is characterized in that: described protein powder is comprised of the raw material of following weight portion:
50～88 parts of soybean isolate protein powders
5～30 parts of PURE WHEYs
3～20 parts of conjugated linoleic acid glyceride microcapsule powders
1～10 part of powder phospholipid;
Wherein, described powder phospholipid is the powder mixture be mixed by 30% lecithin, 24% cephalin and 16% cuorin;
The preparation method of described protein powder:
1) weighing:
Take respectively each raw material according to above-mentioned formula ratio;
2) thick mixed
The mode that powdered phospholipid is increased progressively with equivalent with the conjugated linoleic acid glyceride microcapsule powder drops in mixer is mixed, and forms once mixture; Then the mode described once mixture increased progressively with equivalent with PURE WHEY is mixed, and forms secondary mixture; The mode more described secondary mixture increased progressively with equivalent with soybean isolate protein powder mixes, and forms mixture three times, in mixed process, controls 18～26 ℃ of temperature, humidity≤50%;
3) sieve
By described three mixtures of mixing, through 40 order stainless steel mesh, by the impurity in raw material, foreign matter, discharge clean;
4) total mixed
Raw material after above-mentioned sieving is again dropped in mixer and fully mixes, control 18～26 ℃ of temperature, humidity≤50%, finally form protein powder;
5) detect
Protein powder is sub-packed in double-layer plastic bag, and sampling detects moisture, passes into ozone in polybag, rocks evenly, tightens respectively the sack of two polybags with band;
6) packing
Carry out packing by detecting qualified protein powder.
2. a kind of functional protein powder that contains conjugated linoleic acid glyceride according to claim 1, it is characterized in that: described protein powder is comprised of the raw material of following weight portion:
50 parts of soybean isolate protein powders
28 parts of PURE WHEYs
20 parts of conjugated linoleic acid glyceride microcapsule powders
2 parts of powder phospholipids.
3. a kind of functional protein powder that contains conjugated linoleic acid glyceride according to claim 1, it is characterized in that: described protein powder is comprised of the raw material of following weight portion:
88 parts of soybean isolate protein powders
6 parts of PURE WHEYs
4 parts of conjugated linoleic acid glyceride microcapsule powders
2 parts of powder phospholipids.
4. a kind of functional protein powder that contains conjugated linoleic acid glyceride according to claim 1, it is characterized in that: described protein powder is comprised of the raw material of following weight portion:
60 parts of soybean isolate protein powders
25 parts of PURE WHEYs
10 parts of conjugated linoleic acid glyceride microcapsule powders
5 parts of powder phospholipids.
5. a kind of functional protein powder that contains conjugated linoleic acid glyceride according to claim 1, it is characterized in that: described protein powder is comprised of the raw material of following weight portion:
60 parts of soybean isolate protein powders
20 parts of PURE WHEYs
17 parts of conjugated linoleic acid glyceride microcapsule powders
3 parts of powder phospholipids.
6. a kind of functional protein powder that contains conjugated linoleic acid glyceride according to claim 1, it is characterized in that: described protein powder is comprised of the raw material of following weight portion:
75 parts of soybean isolate protein powders
5 parts of PURE WHEYs
14 parts of conjugated linoleic acid glyceride microcapsule powders
6 parts of powder phospholipids.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310137493.9A CN103190630B (en) | 2013-04-19 | 2013-04-19 | A kind of functional protein powder containing conjugated linoleic acid glyceride |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310137493.9A CN103190630B (en) | 2013-04-19 | 2013-04-19 | A kind of functional protein powder containing conjugated linoleic acid glyceride |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103190630A true CN103190630A (en) | 2013-07-10 |
| CN103190630B CN103190630B (en) | 2015-08-12 |
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103584117A (en) * | 2013-10-17 | 2014-02-19 | 宿州市皖神面制品有限公司 | Method for producing silkworm chrysalis protein powder |
| CN105394182A (en) * | 2015-10-27 | 2016-03-16 | 江西美庐乳业集团有限公司 | Nutritional steamed stuffed bun with blood sugar reducing and heart and cerebral vessel health promoting efficacy |
| CN105533713A (en) * | 2015-12-29 | 2016-05-04 | 广州市戴菊科技发展有限公司 | Medical food with anti-ageing function and preparation method of medical food |
| CN106509126A (en) * | 2016-10-25 | 2017-03-22 | 郑州福润德食品有限公司 | Solid drink having weight losing and body slimming functions |
| CN107485019A (en) * | 2017-08-16 | 2017-12-19 | 东北农业大学 | It is a kind of quick to regain one's strength and increase the compound health powder of flesh |
| CN108514119A (en) * | 2018-03-30 | 2018-09-11 | 北京斯利安药业有限公司 | A kind of product of composition, its application and fat-reducing, increasing flesh |
| CN108703375A (en) * | 2018-04-04 | 2018-10-26 | 大连医诺生物股份有限公司 | Weight losing function food containing conjugated linoleic acid |
| CN108741029A (en) * | 2018-05-28 | 2018-11-06 | 广州正广生物科技有限公司 | High fat diet nutrient powder and preparation method thereof |
| CN111713696A (en) * | 2020-07-21 | 2020-09-29 | 本元大健康科技产业(广东)有限公司 | Weight management meal replacement food and preparation method thereof |
| CN112889987A (en) * | 2019-11-19 | 2021-06-04 | 内蒙古蒙牛乳业（集团）股份有限公司 | Food composition, tablet candy and application |
| CN113424962A (en) * | 2020-09-21 | 2021-09-24 | 黑龙江飞鹤乳业有限公司 | Nutrient component for improving immunity, food or health-care product containing nutrient component and application of nutrient component |
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| CN1357407A (en) * | 2001-10-24 | 2002-07-10 | 中国科学院新疆化学研究所 | Conjugate linoleic acid or conjugate linoleate microcapsule |
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Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103584117A (en) * | 2013-10-17 | 2014-02-19 | 宿州市皖神面制品有限公司 | Method for producing silkworm chrysalis protein powder |
| CN105394182A (en) * | 2015-10-27 | 2016-03-16 | 江西美庐乳业集团有限公司 | Nutritional steamed stuffed bun with blood sugar reducing and heart and cerebral vessel health promoting efficacy |
| CN105533713A (en) * | 2015-12-29 | 2016-05-04 | 广州市戴菊科技发展有限公司 | Medical food with anti-ageing function and preparation method of medical food |
| CN106509126A (en) * | 2016-10-25 | 2017-03-22 | 郑州福润德食品有限公司 | Solid drink having weight losing and body slimming functions |
| CN107485019A (en) * | 2017-08-16 | 2017-12-19 | 东北农业大学 | It is a kind of quick to regain one's strength and increase the compound health powder of flesh |
| CN107485019B (en) * | 2017-08-16 | 2021-01-12 | 东北农业大学 | Composite body building powder for quickly recovering physical strength and enhancing muscle |
| CN108514119A (en) * | 2018-03-30 | 2018-09-11 | 北京斯利安药业有限公司 | A kind of product of composition, its application and fat-reducing, increasing flesh |
| CN108703375A (en) * | 2018-04-04 | 2018-10-26 | 大连医诺生物股份有限公司 | Weight losing function food containing conjugated linoleic acid |
| CN108741029A (en) * | 2018-05-28 | 2018-11-06 | 广州正广生物科技有限公司 | High fat diet nutrient powder and preparation method thereof |
| CN112889987A (en) * | 2019-11-19 | 2021-06-04 | 内蒙古蒙牛乳业（集团）股份有限公司 | Food composition, tablet candy and application |
| CN111713696A (en) * | 2020-07-21 | 2020-09-29 | 本元大健康科技产业(广东)有限公司 | Weight management meal replacement food and preparation method thereof |
| CN113424962A (en) * | 2020-09-21 | 2021-09-24 | 黑龙江飞鹤乳业有限公司 | Nutrient component for improving immunity, food or health-care product containing nutrient component and application of nutrient component |
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| CN103190630B (en) | 2015-08-12 |
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