CN103416608B - Experimental-type high-fat primate feed and preparation method thereof - Google Patents
Experimental-type high-fat primate feed and preparation method thereof Download PDFInfo
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Abstract
The invention discloses an experimental-type high-fat primate feed and a preparation method thereof. The high-fat primate feed is made from the following raw materials in parts by weight: 70-75 parts of basal feed, 20-30 parts of lard, 20-28 parts of vegetable oil, 1-3 parts of albumen and 1-3 parts of cane sugar. The formula of the high-fat primate feed comprises the animal fat lard and a considerable proportion of vegetable oil, and the grease content of the high-fat primate feed can be up to 28.8-51.8%. Therefore, the mouthfeel of the high-fat primate feed is good. A feeder only needs to put the high-fat primate feed in a feeding plate, a monkey can take the high-fat primate feed. Therefore, the high-fat primate feed provides convenience for the feeder, and the feeding of the high-fat primate feed is easy. Besides, the high-fat primate feed can be used for feeding primates for a long time; a real and natural biotic environment for primates to simulate human ingesting high-fat foods is constructed. The high-fat primate feed has the advantages that the components are relatively simple, interference is less, the variations of both blood glucose and blood lipid can be measured simultaneously, so that medical evaluation results are relatively objective and real.
Description
Technical field
The present invention relates to medical care evaluation, detection technique field, be specifically related to a kind of primate test-type high lipid food and preparation method thereof.
Background technology
Along with living standard improves day by day, people are edible high-fat foods in a large number, and the hyperlipemia incidence of disease is raising year by year, and massive epidemiology is investigated and research all shows, hyperlipemia is the hazards that cause the various diseases such as atherosclerotic coronary heart disease, diabetes.Therefore, illustrate the pathogenesis of hyperlipidemia, explore effective prevention of hyperlipemia and treat the research of its relevant disease and control significant.And the generation of human diseases development is very complicated, the progress of medical research usually depends on animal model as experimental basis, but hyperlipemia not yet obviously breaks through desirable animal model preparation so far.
At present, when setting up hyperlipemia animal model, chemical substance induction is the most conventional, especially high lipid food.In the formula of high lipid food, except basal feed, add cholesterol and the 5-10% fat (as lard) of 5-10%, in order to promote the formation of hyperlipemia, also add 0.5-1% dexycholate 0.5-1% first (third) base thiouracil etc.As the application number Chinese invention patent that is 201110008891.1, a kind of high lipid food is disclosed, this feed is comprised of the raw material of following mass percent: rat is bred as feed 80.5%, egg 10%, lard 7%, cholesterol 2%, no. 3 bile salt 0.5%, can be used for building NASH animal model.(the preliminary foundation of < < experimental hyperlipidemia rhesus macaque model and the analysis > > such as Yang Fengmei, China's comparative medicine magazine, 2012, 22(9): 31-35) by high lipid food diet induced method, tentatively set up experimental hyperlipidemia rhesus macaque model and its blood lipids index variation tendency is analyzed, for the research of mankind's hyperlipemia provides animal model, this experiment adopts a kind of high lipid food, this feed is by corn 10%, wheat 10%, dregs of beans 25%, fish meal 5%, sucrose 20%, milk powder 10%, calcium monohydrogen phosphate 1%, premix 4%, animal oil 15% forms.But in above-mentioned high lipid food, the content of fat is still low compared with the human body content of fat of ingesting in high-fat food, and the fat constituent adding is more single, fails to imitate the real fatty picked-up situation of human body, or composition is too complicated, and Interference Detection, may mislead medical care evaluation.
Primate and human gene homology are high, and immune response is similar to people, and in all animals, physiological and biochemical index is the most similar to the mankind.But the fat tolerant power of the mankind and primate there are differences, when human body is ingested the food of high fat content, can't show obvious sense of discomfort, and micro is during to equivalent picked-up content, primate biology cannot be stood the include high levels of fat tend under same condition, shows significantly inadaptable; In behavior, show and abandon food, the situation such as even do not ingest.Existing domestic and international research institution mainly contains two kinds of situations of ingesting: 1. existing product is not suitable for experiment needs, existing product mainly concentrates on conventional aspect, mainly with fermentation series products such as steamed sponge cake, steamed buns, the nutriments such as this series products body volume is huge, the interior fat content of unit volume are few, are not suitable for animal edible, or fermented foodstuff produces acid, produce the flavour that animal is not liked, cannot quantize food ration, make experimental data misalignment; 2. esophagus is filled out and is fed and intravenous injection, animal is very painful in the process of feeding, run counter to humanitarian research morals, the experimentation environment of cannot the real simulation mankind naturally ingesting, also can cause animal esophagus, stomach and other physical skills generation mutation, even pathology, further authenticity, reliability and the validity of animal experimental data will be affected, in filling out the process of feeding, need to employ fill out feed, several researcher's compounding practices such as crawl, waste a large amount of manpower and materials.
Summary of the invention
The object of the invention is the primate high lipid food that is equal to the high fat intake of the mankind at home and abroad there is no at present, a kind of primate test-type high lipid food and preparation method thereof is provided, in order to the true natural biotic environment that provides primate simulating human to ingest high-fat foods, progressively, the induction of metabolic alterations, Physiology and biochemistry situation and the relevant disease such as the blood sugar of long-term observation primate high lipid food after taking in, blood fat, to the relevant medical research of people and high-fat foods metabolism, give targeted, authentic and valid product support.
The technical solution adopted in the present invention is as follows:
A primate test-type high lipid food, comprises the raw material of following weight part ratio: basal feed 70-75 part, lard 20-30 part, vegetable oil 20-28 part, egg white 1-3 part, sucrose 1-3 part.
As preferably, above-described primate test-type high lipid food, comprises the raw material of following weight part ratio: 73 parts of basal feeds, 26 parts of lards, 24 parts of vegetable oil, 2 parts, egg white, 2 parts of sucrose.
Described primate test-type high lipid food can also add appropriate flavoring essence in formula, as: the essence such as banana, Belgium's chocolate, honey peach etc., improve food odors, make primate prefer to eat.In described raw material, preferably include 0.05-0.70 part flavoring essence.
Described lard extracts from pork, solidifies out into solid-state under normal temperature.The grease that described vegetable oil obtains from the fruit of plant, seed, plumule, normal temperature is liquid, as peanut oil, soya-bean oil, linseed oil, castor oil, vegetable seeds wet goods, can be the mixture of several different vegetable oil.Described egg white is the egg white part that land animal is laid eggs, as: the egg white of egg, duck's egg or goose egg etc.
Described basal feed is the normal diet of the daily raising of primate, can guarantee the growth of primate, movable required energy, as the primate feed that can buy on market.Preferably contain the nutritional labeling of following percentage by weight: crude protein >=15%, crude fat >=4.5%, crude fibre≤4%, coarse ash≤7%, calcium 1.0-1.6%, phosphorus 0.7-1.2%.
Preferred as the best, described basal feed comprises the raw material of following percentage by weight: 570 parts, rice, 80 parts, flour, 150 parts of dregs of beans, 45 parts of soybean, 40 parts of FSPCs, 20 parts of fish meal, 50 parts of chicken meals, 8 parts, soya-bean oil, 12 parts of calcium monohydrogen phosphates, 1.5 parts of Choline Chlorides, 3.6 parts of Paris whites, 0.15 part of calcium pantothenate, 3.7 parts of salt, 0.2 part of vitamin C, 0.1 part of vitamin E.
The preparation method of primate test-type high lipid food described in more than one, comprises the following steps:
1. get lard 20-30 part, vegetable oil 20-28 part, egg white 1-3 part, sucrose 1-3 part, stir, mix, obtain mixture;
2. mixture is joined in 70-75 part basal feed, then add suitable quantity of water, stir into uniform biscuit batter, continue to rub and make it to become dough, dough is cut into the blockage of equivalent, put into baking box and dry, take out, obtain.
In described step 1, also add 0.05-0.70 part flavoring essence, to improve food odors.
The preparation method of the above primate test-type high lipid food, the preparation method of described basal feed is:
1. get rice, soybean, dregs of beans, pulverize respectively, sieve, the rice meal that must crush, soy meal, bean cake powder;
2. get 0.1 part of vitamin E, 8 parts, soya-bean oil, mix, by equivalent incremental method, add flour, mix, sieve, obtain finely dispersed flour mixture A;
3. get 1.5 parts of Choline Chlorides, 0.2 part of vitamin C, 0.15 part of calcium pantothenate, mix, add 3.6 parts of stone flours, mix, then add 3.7 parts of salt, 12 parts of calcium monohydrogen phosphates, mix, obtain mixture B;
4. get 45 parts of soy meals, 40 parts of FSPCs, 20 parts of fish meal, 50 parts of chicken meals and mixture A, B, mix, obtain mixture C;
5. get 570 parts of rice meals, 150 parts of bean cake powders and mixture C, put in mixer and be uniformly mixed, the baking oven of putting into 100 ℃ is dried to moisture≤10%, takes out, and obtains.
The invention has the beneficial effects as follows:
1. the grease in human foods is mainly oil & fat, in primate high lipid food formula of the present invention, contain animal tallow lard, also has the vegetable oil of significant proportion simultaneously, in formula, the content of grease is up to 28.8-51.8%, higher than other high lipid foods, in feed, contain sucrose, mouthfeel is good, the person of feeding only need put into high lipid food to take food dish, monkey just can oneself be taken, and animal has good compliance to it, convenient, easy to operate, can feed for a long time, build the ingest true natural biotic environment of high-fat foods of primate simulating human.
2. the patient of hyperlipemia is often accompanied by hyperglycaemia, and in modern's diet, generally eat the food of high sugared content, blood sugar and blood fat in close relations, therefore contain sucrose 1-3 part in primate high lipid food formula of the present invention, similar to mankind's situation of ingesting, can measure the situation of change of blood sugar simultaneously, explore the relation of blood fat and blood sugar.
3. high lipid food formula of the present invention is only used egg white, can get rid of the impact of other compositions on measurement result such as cholesterol in yolk, makes more objective reality of medical care evaluation result.
4. the preparations shaping that the sucrose that the present invention adds, egg white are conducive to feed, the finished product made is glossy, flexible, and figuration is good, animal captures in feed and edible process not easily broken, the present invention does not need to add other auxiliary material again, saves production cost, simple to operate.
5. formula for a product is fixed, and is cut into quantitative bulk, facilitates primate to take food and poultry raiser feeds counting, make that animal capable is disposable eats up given quantity of food, not loss, is convenient to quantitatively to food, thus the content of accurate recording picked-up lipid, sucrose.
The specific embodiment
Below in conjunction with specific embodiment, the invention will be further described, but do not limit the scope of the invention and range of application:
One, the preparation of basal feed
The preparation method of primate basal feed, comprises the following steps:
1. get rice, soybean, dregs of beans, pulverize respectively, cross 60 mesh sieves, the rice meal that must crush, soy meal, bean cake powder;
2. get vitamin E 0.1kg, soya-bean oil 8kg, mix, by equivalent incremental method, add flour, mix, cross 60 mesh sieves, obtain finely dispersed flour mixture A;
3. get Choline Chloride 1.5kg, vitamin C 0.2kg, calcium pantothenate 0.15kg, mix, add stone flour 3.6kg, mix, then add salt 3.7kg, calcium monohydrogen phosphate 12kg, mix, obtain mixture B;
4. get soy meal 45kg, FSPC 40kg, fish meal 20kg, chicken meal 50kg and mixture A, B, mix, obtain mixture C;
5. get rice meal 570kg, bean cake powder 150kg and mixture C, put in mixer and be uniformly mixed, the baking oven of putting into 100 ℃ is dried to moisture≤10%, takes out, and obtains.
Above basal feed contains the nutritional labeling of following percentage by weight: crude protein 19.5%, crude fat 7.2%, crude fibre≤3.8%, coarse ash≤4.5%, calcium 1.3%, phosphorus 0.9%.
Two, the preparation of primate test-type high lipid food
Embodiment 1
The preparation method of primate test-type high lipid food, comprises the following steps:
Get lard 20kg, peanut oil 20kg, egg 1kg, sucrose 1kg, stir, mix, join in the above-mentioned basal feed preparing of 70kg, then add suitable quantity of water, stir into uniform biscuit batter, continue to rub and make it to become dough, dough is cut into the blockage of equivalent, put into the baking box of 180 ℃ and dry, take out, obtain.
Embodiment 2
The preparation method of primate test-type high lipid food, comprises the following steps:
Get lard 30kg, tea oil 28kg, pale blue 3kg, sucrose 3kg, banana flavoring essence 90g, stir, mix, join in the above-mentioned basal feed preparing of 75kg, then add suitable quantity of water, stir into uniform biscuit batter, continue to rub and make it to become dough, dough is cut into the blockage of equivalent, put into the baking box of 180 ℃ and dry, take out, obtain.
Embodiment 3
The preparation method of primate test-type high lipid food, comprises the following steps:
Get lard 26kg, peanut oil 24kg, egg 2k g, sucrose 2kg, stir, mix, join in the above-mentioned basal feed preparing of 73kg, then add suitable quantity of water, stir into uniform biscuit batter, continue to rub and make it to become dough, dough is cut into the blockage of equivalent, put into the baking box of 180 ℃ and dry, take out, obtain.
Three, the situation of feeding of primate test-type high lipid food
By above 3 primate test-type high lipid food embodiment samples, be respectively used to raise rhesus macaque, machin.Sample is put into and taken food dish, and monkey just can oneself be taken, and monkey takes food rapidly, puts into fast food capsule, in case steal, it serves to show animal liking feed of the present invention.After taking in feed, can measure the metabolic alterations such as blood sugar, blood fat, the physiological reaction of simulation human body to high-fat foods.
Claims (8)
1. a primate test-type high lipid food, is characterized in that, comprises the raw material of following weight part ratio: basal feed 70-75 part, lard 20-30 part, vegetable oil 20-28 part, egg white 1-3 part, sucrose 1-3 part.
2. primate test-type high lipid food according to claim 1, is characterized in that, comprises the raw material of following weight part ratio: 73 parts of basal feeds, 26 parts of lards, 24 parts of vegetable oil, 2 parts, egg white, 2 parts of sucrose.
3. primate test-type high lipid food according to claim 1, is characterized in that, also comprises 0.05-0.70 part flavoring essence in described raw material.
4. according to arbitrary described primate test-type high lipid food in claim 1-3, it is characterized in that, described basal feed is containing the nutritional labeling of following percentage by weight: crude protein >=15%, crude fat >=4.5%, crude fibre≤4%, coarse ash≤7%,
Calcium 1.0-1.6%, phosphorus 0.7-1.2%.
5. primate test-type high lipid food according to claim 4, it is characterized in that, described basal feed comprises the raw material of following percentage by weight: 570 parts, rice, 80 parts, flour, 150 parts of dregs of beans, 45 parts of soybean, 40 parts of FSPCs, 20 parts of fish meal, 50 parts of chicken meals, 8 parts, soya-bean oil, 12 parts of calcium monohydrogen phosphates, 1.5 parts of Choline Chlorides, 3.6 parts of Paris whites, 0.15 part of calcium pantothenate, 3.7 parts of salt, 0.2 part of vitamin C, 0.1 part of vitamin E.
6. a preparation method for primate test-type high lipid food as claimed in claim 1 or 2, is characterized in that, comprises the following steps:
(1) get lard 20-30 part, vegetable oil 20-28 part, egg white 1-3 part, sucrose 1-3 part, stir, mix, obtain mixture;
(2) mixture is joined in 70-75 part basal feed, then add suitable quantity of water, stir into uniform biscuit batter, continue to rub and make it to become dough, dough is cut into the blockage of equivalent, put into baking box and dry, take out, obtain.
7. the preparation method of primate test-type high lipid food according to claim 6, is characterized in that, also adds 0.05-0.70 part flavoring essence in described step (1).
8. the preparation method of primate test-type high lipid food according to claim 6, is characterized in that, the preparation method of described basal feed is:
(1) get rice, soybean, dregs of beans, pulverize respectively, sieve, the rice meal that must crush, soy meal, bean cake powder;
(2) get 0.1 part of vitamin E, 8 parts, soya-bean oil, mix, by equivalent incremental method, add flour, mix, sieve, obtain finely dispersed flour mixture A;
(3) get 1.5 parts of Choline Chlorides, 0.2 part of vitamin C, 0.15 part of calcium pantothenate, mix, add 3.6 parts of stone flours, mix, then add 3.7 parts of salt, 12 parts of calcium monohydrogen phosphates, mix, obtain mixture B;
(4) get 45 parts of soy meals, 40 parts of FSPCs, 20 parts of fish meal, 50 parts of chicken meals and mixture A, B, mix, obtain mixture C;
(5) get 570 parts of rice meals, 150 parts of bean cake powders and mixture C, put in mixer and be uniformly mixed, the baking oven of putting into 100 ℃ is dried to moisture≤10%, takes out, and obtains.
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| CN103815128B (en) * | 2014-02-20 | 2015-07-15 | 四川普莱美生物科技有限公司 | High-calorie feed for establishing atherosclerotic animal model and application thereof |
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| CN104799103A (en) * | 2015-05-04 | 2015-07-29 | 广西桂东灵长类开发实验有限公司 | Formula feed for old cynomolgus monkeys in spring and summer and method for preparing feed |
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| CN106212858A (en) * | 2016-07-15 | 2016-12-14 | 广州市饲料研究所 | A kind of high lipid food and animal model of diabetes mellitus type II |
| CN109123109A (en) * | 2018-07-23 | 2019-01-04 | 广东省实验动物监测所 | A kind of primate high glucose and high fat feed and its preparation method and application |
| CN111328920A (en) * | 2020-03-10 | 2020-06-26 | 昆明科灵生物科技有限公司 | Feed for constructing non-alcoholic steatohepatitis model of non-human primate and using method thereof |
| CN113812378B (en) * | 2021-09-29 | 2023-02-03 | 湖北天勤生物技术研究院有限公司 | Method for establishing cynomolgus monkey obesity model and application thereof |
| CN115843937A (en) * | 2022-11-08 | 2023-03-28 | 希诺谷(江苏)生物科技有限公司 | High-fat feed for dogs as well as preparation method and application of high-fat feed |
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