CN106212858A - A kind of high lipid food and animal model of diabetes mellitus type II - Google Patents
A kind of high lipid food and animal model of diabetes mellitus type II Download PDFInfo
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- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 19
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- 241000700159 Rattus Species 0.000 description 3
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 3
- 230000037396 body weight Effects 0.000 description 3
- 238000011697 diabetes animal model Methods 0.000 description 3
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- 230000003442 weekly effect Effects 0.000 description 3
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- HIMXGTXNXJYFGB-UHFFFAOYSA-N alloxan Chemical compound O=C1NC(=O)C(=O)C(=O)N1 HIMXGTXNXJYFGB-UHFFFAOYSA-N 0.000 description 2
- 230000036528 appetite Effects 0.000 description 2
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- 238000010241 blood sampling Methods 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
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- 229940093181 glucose injection Drugs 0.000 description 2
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- 108090000623 proteins and genes Proteins 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
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- 206010006100 Bradykinesia Diseases 0.000 description 1
- 208000024172 Cardiovascular disease Diseases 0.000 description 1
- 208000002705 Glucose Intolerance Diseases 0.000 description 1
- 208000006083 Hypokinesia Diseases 0.000 description 1
- 240000007466 Lilium auratum Species 0.000 description 1
- 235000002159 Lilium auratum Nutrition 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 241000288906 Primates Species 0.000 description 1
- ZSJLQEPLLKMAKR-UHFFFAOYSA-N Streptozotocin Natural products O=NN(C)C(=O)NC1C(O)OC(CO)C(O)C1O ZSJLQEPLLKMAKR-UHFFFAOYSA-N 0.000 description 1
- 241000282898 Sus scrofa Species 0.000 description 1
- 238000013295 T2DM animal model Methods 0.000 description 1
- 210000000227 basophil cell of anterior lobe of hypophysis Anatomy 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
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- 230000035622 drinking Effects 0.000 description 1
- 230000002526 effect on cardiovascular system Effects 0.000 description 1
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- 210000003608 fece Anatomy 0.000 description 1
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- 229940090044 injection Drugs 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
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- 238000001990 intravenous administration Methods 0.000 description 1
- 239000010871 livestock manure Substances 0.000 description 1
- 230000005906 menstruation Effects 0.000 description 1
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- 230000008506 pathogenesis Effects 0.000 description 1
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- 201000009104 prediabetes syndrome Diseases 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 238000011552 rat model Methods 0.000 description 1
- ZSJLQEPLLKMAKR-GKHCUFPYSA-N streptozocin Chemical compound O=NN(C)C(=O)N[C@H]1[C@@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O ZSJLQEPLLKMAKR-GKHCUFPYSA-N 0.000 description 1
- 229960001052 streptozocin Drugs 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/02—Breeding vertebrates
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Fodder In General (AREA)
Abstract
The invention discloses a kind of high lipid food and animal model of diabetes mellitus type II, the raw material of high lipid food includes Semen Maydis, puffing Semen sojae atricolor, Testa Tritici, fish flour, flour, alfalfa meal, Adeps Sus domestica, cholesterol, white sugar, vitamin premix and mineral substance premix.This high lipid food can induce Rhesus Macacus to produce the symptom of type ii diabetes, thus avoids the drug-induced injury producing animal body, and the interference effect of antagonism type ii diabetes medicament research and development.Drug-induced easily there are the phenomenons such as dose is not enough or excessive, and meals induction effectively avoids the generation of these problems.And the model produced has sickness rate height, length of holding time, and more stable, animal is difficult to the feature of death.For the mankind, the more preferably research of type ii diabetes is provided the animal pattern of standard.
Description
Technical field
The present invention relates to animal model preparing technical field, be specifically related to a kind of high lipid food for modeling and II type sugar
The sick animal model of urine.
Background technology
Diabetes (DM) are the third-largest diseases being only second to cardiovascular and cerebrovascular disease, tumor in the world, and in China, sickness rate is gone up year by year
Rise.Diabetes are a kind of commonly encountered diseases, due to insufficient insulin or/and insulin action (opposing) and cause blood glucose to increase and other
Metabolism disorder, in its paathogenic factor, inherited genetic factors and environmental factors respectively account for 50%.In China patient groups, type i diabetes
Accounting for 5.6%, type ii diabetes accounts for 93.7%, and other class patients with type Ⅰ DM only accounts for 0.7%.
Type ii diabetes (DM2) refers to and show with defect of insulin secretion and insulin resistant for main pathophysiological basis
Write feature, cause a paradiabetes of internal glucose and lipid metabolic disorder.Type ii diabetes (T2DM) is E&H
Coefficient result.The pathogenesis of research T2DM and potential treatment means, need suitable T2DM animal model.
Diabetes animal model in scientific research is generally divided into spontaneous and manual-induced two classes of heredity, heredity spontaneous animal models
Such as BB Mus, NOD Mus, db/db Mus, NYS Mus etc.；Manual-induced diabetes animal model is mainly by rat injected chemical medicine
Such as streptozotocin or alloxan, selective destruction beta Cell of islet, cause necrocytosis, cause blood insulin in various degree
Decline and blood glucose raises, but formation is type Ⅰ diabetes mellitus animal model.Also have been reported that and define type ii diabetes animal with rat
Model (foundation of rats with type 2 diabetes animal model, Mu Songniu etc., Jiangxi Medical College's journal the 8th phase of volume 49 in 2009).
Farther out, there is a definite limitation in model applicability, the most current manual-induced mode for rat and human inheritance's relation
The many employings of Animal diseases modeling are drug-induced, and animal is produced bigger injury.
Summary of the invention
On the one hand the purpose of the present invention is to provide a kind of high lipid food, and its at least one purposes is that feeding animals carries out sugar
The sick modeling of urine.
Another object of the present invention is to provide a kind of new type ii diabetes animal model, preferably to study mankind II
Patients with type Ⅰ DM.
The high lipid food that the present invention provides, raw material includes Semen Maydis, puffing Semen sojae atricolor, Testa Tritici, fish flour, flour, alfalfa meal, pig
Oil, cholesterol, white sugar, vitamin premix and mineral substance premix.Add Adeps Sus domestica and white sugar, make the fat content of feedstuff
Increasing with the content of sugar, the feedstuff of high glucose and high fat is more conducive to modeling.
Preferably, in above-mentioned high lipid food, raw material based on weight/mass percentage composition, Semen Maydis 20～44%, puffing Semen sojae atricolor 18～
35%, Testa Tritici 2～4%, fish flour 7～10%, flour 10～18%, alfalfa meal 1～4%, Adeps Sus domestica 9～21%, cholesterol 1～
1.5%, white sugar 5～7%, vitamin premix 0.05～0.15%, mineral substance premix 2～4%.
Preferably, in above-mentioned high lipid food, raw material based on weight/mass percentage composition, Semen Maydis 32.5%, puffing Semen sojae atricolor 22%, wheat
Bran 2%, fish flour 7%, flour 14%, alfalfa meal 2%, Adeps Sus domestica 12%, cholesterol 1%, white sugar 5%, vitamin premix
0.05%, mineral substance premix 2%.
In feedstuff, protein content is about 17%, and fat content is about 20%, and according to GB 14924.3-2010, monkey maintains the phase
Forage protein content should be more than or equal to 16%, and fat content is more than or equal to 4%.Prepare such nutrient composition content, can
Keep the orthobiosis of monkey, facilitate again modeling.
Present invention also offers above-mentioned high lipid food preparation method, be first by Semen Maydis, puffing Semen sojae atricolor, Testa Tritici, fish flour, flour
Pulverize respectively with alfalfa meal, cross 80 mesh sieves, evenly mixing device adds after mixing cholesterol, white sugar, vitamin premix
Continue mixing with mineral substance premix, be eventually adding Adeps Sus domestica and suitable quantity of water, make ball of glutinous rice shape.
The animal model of diabetes mellitus type II that the present invention provides, is that the high lipid food fed described in any of the above with Rhesus Macacus is made
Mould forms.
Non-human primate due on race relation with people close to it is considered to be closest to human diseases diagnostic cast
Type animal.The present invention selects Rhesus Macacus to be laboratory animal.Many features of Rhesus Macacus make it particularly appropriate for use in and carry out T2DM's
Research, their build is relatively large, easy artificial house, and the life-span was at about 25 years, and these features make them suitable to long
The research that the diet of phase and disease prevention are intervened.
Preferably, above-mentioned animal model of diabetes mellitus type II, described Rhesus Macacus is public monkey.Menstruation in order to avoid the female monkey of impact
Cycle, so animal is more preferable based on using public monkey.
Preferably, above-mentioned animal model of diabetes mellitus type II, the described Rhesus Macacus age is between 8～12 years old.This age bracket
Belonging to person in middle and old age's period of monkey, select the monkey of this age bracket to be easier to type Ⅱdiabetes mellitus, modeling is easier to.
Preferably, above-mentioned animal model of diabetes mellitus type II, described rhesus monkeys focuses between 9～14kg, and build is the most fat
Monkey is more conducive to modeling.
Preferably, above-mentioned animal model of diabetes mellitus type II, concrete feeding method is: it is enough that every morning gives Rhesus Macacus
High lipid food；Give Fructus Mali pumilae or vegetable afternoon；Feed process 10 weeks by a definite date.The amount of Fructus Mali pumilae or vegetable can be fitted according to the situation of searching for food
Work as adjustment.
To enough high lipid foods, wherein said refers to that Rhesus Macacus can arbitrarily obtain, constantly measure.
Preferably, above-mentioned animal model of diabetes mellitus type II, before most starting modeling, the fasting glucose (FPG) of described Rhesus Macacus
Less than 5mmol/L (90mg/dL).Before selecting to be present to ensure that Rhesus Macacus modeling less than the Rhesus Macacus of this blood glucose value, blood glucose is normal,
Symptom without type Ⅱdiabetes mellitus.
Compared with prior art, the method have the advantages that
The high lipid food of the present invention can induce Rhesus Macacus to produce the symptom of type ii diabetes, thus avoids drug-induced to dynamic
The injury that thing body produces, and the interference effect of antagonism type ii diabetes medicament research and development.Drug-induced easily occur that dose is not enough
Or the phenomenons such as excess, meals induction effectively avoids the generation of these problems.And the model produced has sickness rate height, dimension
Time of holding is long, more stable, and animal is difficult to the feature of death.For the mankind, the more preferably research of type ii diabetes is provided the mould of standard
Type animal.
Detailed description of the invention
Below in conjunction with specific embodiment, the invention will be further described, so that those skilled in the art can be more preferable
Understand the present invention and can be practiced, but illustrated embodiment is not as a limitation of the invention.
The preparation of embodiment 1 high lipid food
High lipid food: Semen Maydis 32.5%, puffing Semen sojae atricolor 22%, Testa Tritici 2%, fish flour 7%, flour 14%, alfalfa meal
2%, Adeps Sus domestica 12%, cholesterol 1%, white sugar 5%, vitamin premix 0.05%, mineral substance premix 2%, Fructus Anisi Stellati is (beautiful
Rice, puffing Semen sojae atricolor, Testa Tritici, fish flour, flour, alfalfa meal etc.) pulverize in pulverizer, cross the sieve of 80 mesh, in mixing dress
Adding cholesterol, white sugar, vitamin and mineral mixing after putting mixing, rear addition Adeps Sus domestica and pure water are sufficiently stirred for, system
Become the feedstuff of ball of glutinous rice shape shape.Forage protein content is 17%, and fat content is 20%.
Mineral substance premix is provided by Guangzhou lilium auratum lindle feed technology company limited, and vitamin premix is liked to protect by Guangzhou
Agriculture feed corporation,Ltd provides.Remaining composition obtains the most from commercial channels.
Embodiment 2 animal model of diabetes mellitus type II
20 Rhesus Macacus for experiment are public monkey, and the age, body weight was between 9～14kg between 8～12 years old.Test
Before, the fasting glucose (FPG) of all animals is below 5mmol/L (90mg/dL).
It is only used as matched group 20 Rhesus Macacus have selected 10, wherein 10 is only used as simple feedstuff induction Rhesus Macacus II type
The model group of diabetes model.
During test, give the enough food of laboratory animal (can arbitrarily obtain) every morning, when quantity of food is according to cultivation
Between adjusted.All give appropriate Fructus Mali pumilae or vegetable in the afternoon.
According to the method described above, experimental group feeds the high lipid food of embodiment 1, the diet of relatively high fat compared with matched group
(comprising the protein of 17%, the fat of 20%).Matched group gives the diet of arm's length standard, and (normal diet is purchased from Guangzhou state
Dragon Science and Technology Ltd., containing the protein of 17%, the fat of 4%).
Experimentation 10 weeks by a definite date.
In 10 weeks that experiment starts, observe every day and record ingest, drink water, urine volume, mental status；Weigh in weekly
1 time；Clinical observation project: detect weekly fasting glucose, triglyceride, T-CHOL, C peptide.
1, ingest
In the experiment incipient stage, first progressively add food, until finding out the substantially appetite of animal.
2, drinking-water
Drinking bottle is tied up on cage limit to claim to survey amount of drinking water.
3, urine volume
Every day 8:30 point and afternoon 4:30 point detection wooden pail for urine in urine volume, the urine volume in manure bucket also to be counted in simultaneously.
4, carbohydrate tolerance
Select in the noon before that day six of detection the food of animal all removed, and with next day according to the body weight of animal according to
4ml/kg injects 50% glucose injection to animals iv, and detects animals with 0min, 10,30,60,90,120min blood samplings
Blood glucose, notes during blood sampling avoiding in the same position repeatable operation of animal and avoids the position of intravenous glucose injection injection.
5, mental status
During feeding every day and will observe the mental status of animal when going to work morning and afternoon, primary part observation animal is with or without appetite not
Shake.
6, body weight
Claim weekly to survey once.
7, clinical observation project
Fasting glucose, triglyceride, T-CHOL, C peptide are detected by Ex-3600 full automatic biochemical apparatus.
Experimental result and discussion:
Result shows: model group food ration, drinking-water and urine volume after modeling all increased, and food ration is after modeling 6 weeks
All with matched group, significant difference occurs, extremely notable (such as table 1) with matched group difference when 10 weeks.
Table 1 food ration Zhou Bianhua table (g)
| Time | Model group | Matched group |
| 0th week | 212±25.3 | 210±28.5 |
| 1st week | 216±28.9 | 218±30.7 |
| 2nd week | 220±29.3 | 224±32.6 |
| 3rd week | 229±30.2 | 230±31.7 |
| 4th week | 234±28.7 | 236±34.8 |
| 5th week | 258±30.4 | 235±29.5 |
| 6th week | 265±30.7* | 229±24.8 |
| 7th week | 262±29.8* | 227±28.6 |
| 8th week | 269±25.4* | 219±23.6 |
| 9th week | 283±29.4* | 225±27.3 |
| 10th week | 309±30.6** | 229±24.7 |
Compared with matched group, * P < 0.05, * * P < 0.01.
The amount of drinking water of model group, after raising 7 weeks, is significantly higher than matched group, extremely notable with the difference of matched group after 8 weeks (as
Table 2).
Table 2 amount of drinking water Zhou Bianhua table (ml)
| Time | Model group | Matched group |
| 0th week | 264±29.5 | 260±30.4 |
| 1st week | 267±30.1 | 263±30.8 |
| 2nd week | 269±29.5 | 265±29.4 |
| 3rd week | 271±31.4 | 267±31.6 |
| 4th week | 277±30.8 | 274±30.1 |
| 5th week | 287±32.5 | 278±32.8 |
| 6th week | 308±35.6 | 279±33.6 |
| 7th week | 367±29.5* | 269±28.5 |
| 8th week | 452±32.7** | 280±34.7 |
| 9th week | 603±34.7** | 285±36.2 |
| 10th week | 659±36.2** | 287±37.2 |
Compared with matched group, * P < 0.05, * * P < 0.01.
The variation tendency of the urine volume of model group is identical with the variation tendency of amount of drinking water, and after 7 weeks, animal occurs that urine volume substantially increases
The trend added.Specifically it is shown in Table 3.
Table 3 urine volume change (ml)
| Time | Model group | Matched group |
| 0th week | 230±24.5 | 235±24.1 |
| 1st week | 234±22.6 | 239±23.2 |
| 2nd week | 239±23.5 | 241±24.6 |
| 3rd week | 247±24.1 | 245±23.8 |
| 4th week | 251±25.1 | 248±24.1 |
| 5th week | 255±23.4 | 246±25.3 |
| 6th week | 275±24.1 | 249±20.1 |
| 7th week | 298±24.8* | 247±23.5 |
| 8th week | 373±29.8** | 248±25.2 |
| 9th week | 502±34.7** | 243±22.8 |
| 10th week | 568±39.5** | 246±26.3 |
Compared with matched group,*P < 0.05,**P<0.01。
Model group weight is below matched group in modeling after 7 weeks, for the weight after the animal 7 weeks of same model group also
Weight (table 4) before being substantially less than 7 weeks.
Table 4 changes of body mass (kg)
Compared with matched group, * P < 0.05, * * P < 0.01.
The C peptide of model group, along with the increase of culture-cycle, presents the trend being gradually lowered.Raising before 8 weeks, model group with
Matched group, and model group there are no significant within different breeding phase difference.When raising 8 weeks, the C peptide of model group is the most right
According to group, concrete data are shown in Table 5.
Table 5C peptide (μ g/L)
| Time | Model group | Matched group |
| 0th week | 3.81±0.81 | 3.78±0.82 |
| 1st week | 3.78±0.80 | 3.72±0.78 |
| 2nd week | 3.69±0.74 | 3.67±0.72 |
| 3rd week | 3.60±0.72 | 3.75±0.70 |
| 4th week | 3.54±0.68 | 3.65±0.68 |
| 5th week | 3.42±0.65 | 3.69±0.64 |
| 6th week | 3.25±0.52 | 3.58±0.62 |
| 7th week | 3.01±0.51 | 3.69±0.70 |
| 8th week | 2.36±0.48* | 3.73±0.72 |
| 9th week | 1.98±0.36* | 3.77±0.74 |
| 10th week | 1.80±0.28* | 3.82±0.76 |
Compared with matched group, * P < 0.05, * * P < 0.01.
Table 6 fasting glucose changes
Compared with matched group, * P < 0.05, * * P < 0.01.
As shown in table 6: the fasting glucose content of model group and matched group are poor without significance before modeling and before cultivation 5 weeks
Different.Being significantly higher than matched group when cultivating 5 weeks, when cultivating 7 weeks and 8 weeks, the difference with matched group reaches pole significant level.From 5
Starting in week to experiment to terminate, the fasting glucose content of model group is all remarkably higher than matched group.
Table 7 T-CHOL change (m mol/L)
| Time | Model group | Matched group |
| 0th week | 2.47±0.32 | 2.48±0.37 |
| 1st week | 2.50±0.33 | 2.51±0.34 |
| 2nd week | 2.59±0.31 | 2.56±0.32 |
| 3rd week | 2.71±0.36 | 2.63±0.39 |
| 4th week | 2.78±0.40 | 2.72±0.41 |
| 5th week | 2.81±0.42 | 2.78±0.40 |
| 6th week | 2.85±0.41 | 2.73±0.39 |
| 7th week | 2.83±0.43 | 2.81±0.42 |
| 8th week | 2.97±0.40 | 2.78±0.36 |
| 9th week | 3.15±0.42* | 2.75±0.35 |
| 10th week | 3.42±0.43* | 2.69±0.31 |
Compared with matched group, * P < 0.05, * * P < 0.01.
As shown in table 7: the cholesterol level of model group there was no significant difference to when cultivating 8 weeks with matched group before modeling.
At the end of cultivating 9 thoughtful experiments, the cholesterol level of model group is significantly higher than matched group.
Table 8 triglyceride change (m mol/L)
Compared with matched group, * P < 0.05, * * P < 0.01.
As shown in table 8, the content of triglyceride of model group presents the trend of increase along with the increase of culture-cycle.Matched group
Not occurring significant difference during cultivating, when cultivating 10 weeks, the content of triglyceride of model group is significantly higher than matched group.
Carbohydrate tolerance (blood glucose value, mmol/L) before table 9 modeling
| Time | Model group | Matched group |
| 0min | 4.13±0.57 | 4.29±0.54 |
| 10min | 12.83±2.10 | 12.79±1.96 |
| 30min | 23.82±6.21 | 24.02±7.01 |
| 60min | 17.61±3.12 | 16.82±2.83 |
| 90min | 10.22±0.80 | 9.87±0.79 |
| 120min | 4.20±0.46 | 4.32±0.46 |
Compared with matched group, * P < 0.05, * * P < 0.01.
Carbohydrate tolerance (blood glucose value, mmol/L) after table 10 modeling
Compared with matched group, * P < 0.05, * * P < 0.01.
Before modeling, the carbohydrate tolerance of animal pattern and matched group is the most normal.After modeling, the animal carbohydrate tolerance of matched group just shows
Often, the impaired glucose tolerance of model group.
The monkey of model group occurs the symptoms such as bradykinesia, puffiness of face, modeling success after cultivating 5 weeks successively.
Embodiment described above is only the preferred embodiment lifted by absolutely proving the present invention, the protection model of the present invention
Enclose and be not limited to this.The equivalent that those skilled in the art are made on the basis of the present invention substitutes or conversion, all in the present invention
Protection domain within.Protection scope of the present invention is as the criterion with claims.
Claims (10)
1. a high lipid food, it is characterised in that raw material include Semen Maydis, puffing Semen sojae atricolor, Testa Tritici, fish flour, flour, alfalfa meal,
Adeps Sus domestica, cholesterol, white sugar, vitamin premix and mineral substance premix.
High lipid food the most according to claim 1, it is characterised in that raw material based on weight/mass percentage composition, Semen Maydis 20～
44%, puffing Semen sojae atricolor 18～35%, Testa Tritici 2～4%, fish flour 7～10%, flour 10～18%, alfalfa meal 1～4%, Adeps Sus domestica
9～21%, cholesterol 1～1.5%, white sugar 5～7%, vitamin premix 0.05～0.15%, mineral substance premix 2～
4%.
High lipid food the most according to claim 1, it is characterised in that raw material based on weight/mass percentage composition, Semen Maydis 32.5%,
Puffing Semen sojae atricolor 22%, Testa Tritici 2%, fish flour 7%, flour 14%, alfalfa meal 2%, Adeps Sus domestica 12%, cholesterol 1%, white sugar
5%, vitamin premix 0.05%, mineral substance premix 2%.
4. the arbitrary described high lipid food preparation method of claims 1 to 3, it is characterised in that be first by Semen Maydis, puffing Semen sojae atricolor,
Testa Tritici, fish flour, flour and alfalfa meal are pulverized respectively, cross 80 mesh sieves, add cholesterol, white sand in evenly mixing device after mixing
Sugar, vitamin premix and mineral substance premix continue mixing, are eventually adding Adeps Sus domestica and suitable quantity of water, make ball of glutinous rice shape.
5. an animal model of diabetes mellitus type II, it is characterised in that feed the arbitrary described height of claims 1 to 3 with Rhesus Macacus
Fat feedstuff modeling forms.
Animal model of diabetes mellitus type II the most according to claim 5, it is characterised in that described Rhesus Macacus is public monkey.
Animal model of diabetes mellitus type II the most according to claim 5, it is characterised in that the described Rhesus Macacus age is 8～12
Between Sui.
Animal model of diabetes mellitus type II the most according to claim 5, it is characterised in that described rhesus monkeys focus on 9～
Between 14kg.
Animal model of diabetes mellitus type II the most according to claim 5, it is characterised in that concrete feeding method is: every day is early
Give the high lipid food that Rhesus Macacus is enough morning；Give Fructus Mali pumilae or vegetable afternoon；Feed process 10 weeks by a definite date.
Animal model of diabetes mellitus type II the most according to claim 9, it is characterised in that before most starting modeling, described Henghe
The fasting glucose of monkey is less than 5mmol/L.
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| CN113057139A (en) * | 2021-04-16 | 2021-07-02 | 扬子江药业集团江苏龙凤堂中药有限公司 | Animal model of infantile anorexia caused by high calorie high fat diet |
| CN113796459A (en) * | 2021-09-13 | 2021-12-17 | 四川省医学科学院.四川省人民医院实验动物研究所 | High-fat feed and application thereof in constructing diabetes animal model |
| CN115736132A (en) * | 2022-10-31 | 2023-03-07 | 宁波大学 | High-fat feed for black porgy and preparation method and application thereof |
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| CN113057139B (en) * | 2021-04-16 | 2023-07-07 | 扬子江药业集团江苏龙凤堂中药有限公司 | Infantile anorexia animal model caused by high calorie and high fat diet |
| CN113796459A (en) * | 2021-09-13 | 2021-12-17 | 四川省医学科学院.四川省人民医院实验动物研究所 | High-fat feed and application thereof in constructing diabetes animal model |
| CN115736132A (en) * | 2022-10-31 | 2023-03-07 | 宁波大学 | High-fat feed for black porgy and preparation method and application thereof |
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