CN102726603A - Method for preparing feed additive by fermentation waste of trollflower dregs - Google Patents

Method for preparing feed additive by fermentation waste of trollflower dregs Download PDF

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Publication number
CN102726603A
CN102726603A CN2012102361880A CN201210236188A CN102726603A CN 102726603 A CN102726603 A CN 102726603A CN 2012102361880 A CN2012102361880 A CN 2012102361880A CN 201210236188 A CN201210236188 A CN 201210236188A CN 102726603 A CN102726603 A CN 102726603A
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dregs
trollflower
decoction
feed additive
feed addictive
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CN102726603B (en
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韩淑英
王志路
储金秀
王建行
胡雷
白静
张博男
韩婷
勾向博
安春娜
王树华
张潇
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Taihang Mountain Shexian County medicinal material industry Co.,Ltd.
North China University of Science and Technology
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Hebei United University
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Abstract

The invention relates to a method for preparing feed additive by fermentation waste of trollflower dregs, belonging to the technical field of prepared traditional Chinese medicine recycling and feed additive. The technical proposal is as follows: drying and smashing the fermentation waste of the trollflower dregs to be used as raw materials; using angel saccharomycete as a fermenting microbe, simultaneously adding peptone, glucose nutritional ingredient and absolute ethyl alcohol for leavening, which are necessary for the growth of saccharomycete to extract the medical active ingredient; using the precipitate part as the feed additive material; drying and smashing the feed additive material to be powder; carrying out radicidation to acquire feed additive, wherein the feed additive is added to the animal diet in the mass ratio of 2-6%. The experimental result shows that the fermentation waste of the trollflower dredges, as feed additive, has higher daily gain effects to experimental animals than those to a control group; the feed consumption rate is not increased, is consistent with that of the control group, and so good effects are shown. The feed additive provides a new feed choice for animal farming, has such characteristics as low cost, convenience in application, high gain and the like.

Description

A kind of method for preparing feed addictive with trollflower dregs of a decoction fermentation wastes
Technical field
The present invention relates to a kind ofly prepare the method for feed addictive, belong to preparation Chinese medicine discarded object and utilize again and field of feed additive technology with trollflower dregs of a decoction fermentation wastes.
Background technology
Chinese medicine is the quintessence of Chinese culture of China, in recent years, along with the approval of the world to Chinese medicine, the output rapid growth of Chinese medicine, the output of the dregs of a decoction also grows with each passing day.The dregs of a decoction are generally wet stock, and very easily corrupt, taste is smelly different.Early stage Chinese medicine slag forms of treatment mainly comprises landfill, burning, FX stacking etc.These processing methods not only exhaust substantial contribution, have more caused the wasting of resources and serious pollution, and how handling Chinese medicine slag has become a problem demanding prompt solution.So, utilize biotechnology that Chinese medicine slag is handled, turn waste into wealth in the hope of reaching, the utilization rate that improves natural resources of Chinese medicinal materials enjoys people's attention.Not only contain nascent materials such as rich in protein, cellulose in the dregs of a decoction; And because present extraction process more single (decocting boils); These still remain in the dregs of a decoction through the part active component in the Chinese medicine after extracting, decocting, and these residual nascent and secondary substances make the dregs of a decoction have very high recycle value, therefore; The dregs of a decoction begin to receive people's attention as a kind of resource, have become one of important content of modernization of cmm.
Trollflower is the dry flower and alabastrum of ranunculaceae plant trollflower (Trollius chinensis Bunge), is distributed widely in areas such as southwest, northwest, North China, northeast.Trollflower has long medicinal history, and among the people infusing with boiling water worked as tea, has clearing heat and detoxicating effect.At present; Domestic have drugmaker of tens of family to produce trollflower Chinese medicine; Preparations such as globeflower capsule, Tabellae Trollii, tropaeolum oral liquid, Chinese globe flower injection are arranged, the clinical inflammation such as treatment flu, fever, chronic tonsillitis, acute myringitis, urinary tract infections that are mainly used in.According to incompletely statistics, the whole nation utilizes the dregs of a decoction of trollflower pharmacy generation that millions of tons are arranged every year, and the overwhelming majority goes out of use, and not only contaminated environment, and pharmaceutical factory also will consume a large amount of expenses for handling the dregs of a decoction.Therefore how the trollflower dregs of a decoction are utilized fully, turn waste into wealth, of far-reaching significance.
Summary of the invention
The purpose of this invention is to provide and a kind ofly prepare the method for feed addictive with trollflower dregs of a decoction fermentation wastes, with low cost, convenient in application, effective, dregs of a decoction discarded object utilizes fully again, and energy-conserving and environment-protective solve the problems referred to above that exist in the background technology.
The objective of the invention is to realize like this:
A kind ofly prepare the method for feed addictive, comprise following processing step with trollflower dregs of a decoction fermentation wastes:
1. the trollflower dregs of a decoction after the drying are ground into powder;
2. the saccharomycete activation and proliferation is cultivated, and the enrichment liquid body of getting behind the autoclaving is cultivated based in the sterile chamber, adds Angel dry ferment 5-10 gram/100ml under the gnotobasis, puts 35-37 ℃ of constant incubator cultivation 5-7 h;
3. the value-added yeast liquid of activation being added the mass and size mark is 40-60% trollflower dregs of a decoction powder, 4% peptone, 4% glucose, mixes the continued fermentation;
5. after the fermentation ends, fermentate is extracted effective medicinal components with absolute ethyl alcohol, process is centrifugal behind the adding absolute ethyl alcohol, contains medicinal ingredient in the supernatant, is used for extracting the preparation medicine, sinks partly as the feed addictive raw material;
6. with 90 ℃ of dryings of above-mentioned feed addictive raw material, grind into powder, radio sterilization is feed addictive.
Described feed addictive is processed granule or pulvis, and feed addictive is added in the livestock and poultry diet by mass ratio 2%-6%.
Describedly get enrichment liquid body culture medium behind the autoclaving in following ratio: peptone 40 g, yeast soak powder 20 g, glucose 40 g, place 1000 ml distilled water.
Feed addictive is applied to carry out zoopery in meat chick, rabbit, the piglet diet, observes its daily gain and daily ingestion amount.The result show trollflower dregs of a decoction fermentation wastes as feed addictive to the animal used as test daily gain apparently higher than control group, the food consumption rate does not increase, and is consistent with control group, shown good effect.Select for animal husbandry provides a kind of new feed, have characteristics such as with low cost, convenient in application, benefit height.
Good effect of the present invention is: (1) this feed is nutritious in adding: because of the trollflower dregs of a decoction and yeast thalline have the compositions useful to body such as rich in protein (containing a large amount of mycoproteins), polysaccharide, each seed amino acid, mineral matter, trace element, vitamin and unrighted acid; These nutritional labelings are insoluble to ethanol; Ethanol can make that it solidifies, centrifugal back sinks, and therefore getting its sediment, to be applied to feed addictive nutritious; (2) raw material resources are abundant: discarded trollflower dregs of a decoction fermented extracted medicinal active ingredient; Again discarded fermentation residue is made full use of; The fundamental state policy that meets sustainable development and recycling economy; All useful to enterprise, consumer, farming and animal husbandry and poultry industry, have popularizing application prospect, can produce economic benefit and social benefit; The enterprise that the trollflower pharmacy is used in the whole nation is a lot, so dregs of a decoction aboundresources, is easy to get, and the present invention provides scientific basis for the utilization again of trollflower resource with new purposes; (3) preparation technology is simple: this method technological process is simple, does not need specific apparatus and equipment, and cost is low, and is with short production cycle, is suitable for suitability for industrialized production; (4) convenient in application, effect is obvious: said preparation is a solid dosage forms, and consumption is little, potency ratio is high, it is all convenient to store and use, and is applied in piglet, meat chick, the rabbit daily ration, and the body weight that increases day by day is apparently higher than control group, and food consumption does not increase; (5) reduce pollution; Increase economic benefit: trollflower dregs of a decoction water content is higher, and is very easily corrupt, can cause serious pollution to environment; And still contain compositions capable of using such as protein, polysaccharide, various trace element and vitamin in the dregs of a decoction fermentation wastes; In time it is carried out comprehensively, makes full use of, not only can the contaminated solution problem, also can bring certain economic benefits; Also solved the high-quality feed shortage problem of restriction China animal husbandry development simultaneously, had broad prospects.
The specific embodiment
Below, the present invention is described further through embodiment.
A kind ofly prepare the method for feed addictive, comprise following processing step with trollflower dregs of a decoction fermentation wastes:
1. the trollflower dregs of a decoction after the drying are ground into powder;
2. the saccharomycete activation and proliferation is cultivated, and the enrichment liquid body of getting behind the autoclaving is cultivated based in the sterile chamber, adds Angel dry ferment 5-10 gram/100ml under the gnotobasis, puts 35-37 ℃ of constant incubator cultivation 5-7 h;
3. the value-added yeast liquid of activation being added the mass and size mark is 40-60% trollflower dregs of a decoction powder, 4% peptone, 4% glucose, mixes the continued fermentation;
4. fermentation condition is 30-37 ℃ of cultivation temperature, incubation time 72-96 hours;
5. after the fermentation ends, fermentate is extracted effective medicinal components with absolute ethyl alcohol, process is centrifugal behind the adding absolute ethyl alcohol, contains medicinal ingredient in the supernatant, is used for extracting the preparation medicine, sinks partly as the feed addictive raw material;
6. with 90 ℃ of dryings of above-mentioned feed addictive raw material, grind into powder, radio sterilization is feed addictive.
In an embodiment, the present invention is fermented the trollflower dregs of a decoction by above-mentioned preparation technology, in the scope of regulation, has carried out the preparation of several schemes, and its tunning has been carried out zoopery, all obtains consistent useful result.
Embodiment one:
1. the saccharomycete activation and proliferation is cultivated: enrichment liquid body culture medium: saccharomycete=100ml:5g, 35 ℃ of constant incubators are cultivated 7 h.
2. activation saccharomycete and trollflower dregs of a decoction ratio: activation yeast liquid: the trollflower dregs of a decoction=100ml:40g.
3. fermentation condition is: 30 ℃ of temperature, 96 hours time.
4. fermentate extracts medicinal ingredient: fermentate adds absolute ethyl alcohol, makes concentration of alcohol reach 60%, 70 ℃ of extraction 2h, 1500 rev/mins of centrifugal 25min of extract.Supernatant contains medicinal composition.
5. centrifugal sinking part is a trollflower dregs of a decoction fermentation wastes, both as feed addictive.
Embodiment two:
1. the saccharomycete activation and proliferation is cultivated: enrichment liquid body culture medium: saccharomycete=100ml:8g, 36 ℃ of constant incubators are cultivated 6 h.
2. activation saccharomycete and trollflower dregs of a decoction ratio: activation yeast liquid: the trollflower dregs of a decoction=100ml:50g.
3. fermentation condition is: 34 ℃ of temperature, 84 hours time.
4. fermentate method for distilling: fermentate adds absolute ethyl alcohol, makes concentration of alcohol reach 70%, 70 ℃ of extraction 4h, 2000 rev/mins of centrifugal 20min of extract.Supernatant contains medicinal composition.
5. centrifugal sinking part is a trollflower dregs of a decoction fermentation wastes, both as feed addictive.
Embodiment three:
1. the saccharomycete activation and proliferation is cultivated: enrichment liquid body culture medium: saccharomycete=100ml:10g, 37 ℃ of constant temperature culture 5 h.
2. activation saccharomycete and trollflower dregs of a decoction ratio: activation yeast liquid: the trollflower dregs of a decoction=100ml:60g.
3. fermentation condition is: 37 ℃ of temperature, 72 hours time.
4. fermentate method for distilling: fermentate adds absolute ethyl alcohol, makes concentration of alcohol reach 80%, 70 ℃ of extraction 3h, 2500 rev/mins of centrifugal 15min of extract.Supernatant contains medicinal composition.
5. centrifugal sinking part is a trollflower dregs of a decoction fermentation wastes, both as feed addictive.
With embodiment one, two, three grind into powders, add in piglet, meat chick and the young rabbit daily ration by 2%, 4%, 6% respectively, its piglet and meat chick daily ration that contains dregs of a decoction fermentation wastes is processed pulvis, the rabbit daily ration is processed granule.
Embodiment one, two, three added in the feed by 2%, 4%, 6% respectively carry out zoopery.
Contain dregs of a decoction fermentation wastes forage feed piglet
40 piglets that the selection hereditary basis is similar, sex is consistent, be in a good state of health, average weight is 20 kg are divided into 4 groups at random, raise term harmonization.Dregs of a decoction fermentation wastes is added in the conventional daily ration of piglet in 2%, 4%, 6% ratio, fed 60 days, establish control group simultaneously, feed conventional daily ration.Weigh during respectively at experiment beginning and end (fixing a point to weigh on an empty stomach), calculate the average daily gain situation morning; Calculate average daily ingestion amount.The result sees table 1.
Table 1 sample to the influence (kg) of piglet average daily gain and daily ingestion amount (
Figure 431912DEST_PATH_IMAGE001
± s, n=10)
Figure 203559DEST_PATH_IMAGE002
Compare * with control group P<0.05, * * P<0.01
Table 1 result shows that along with the increase of sample size, piglet daily gain value also increases, each sample group increase day by day weight average apparently higher than control group ( P<0.05, P<0.01), and daily ingestion amount does not increase, daily ingestion amount indifference (P between each tissue; 0.05).
2. Contain dregs of a decoction fermentation wastes forage feed meat chick
15 age in days fryer, male and female half and half are selected in experiment for use.Fermentate is added in the chicken feed diet in 2%, 4%, 6% ratio, and its meat chick daily ration that contains dregs of a decoction fermentation wastes is processed pulvis, is contrast with the basal diet that does not add dregs of a decoction fermentation wastes, and 20 every group, the observation period is limited to 30 days.Weigh during respectively at experiment beginning and end (fixing a point to weigh on an empty stomach), calculate the average daily gain situation morning; Calculate average daily ingestion amount.The result sees table 2.
Table 2 sample to the influence (g) of average daily gain of meat chick and daily ingestion amount (
Figure 91880DEST_PATH_IMAGE001
± s, n=20)
Figure 974386DEST_PATH_IMAGE003
Compare * with control group P<0.05, * * P<0.01
Table 2 result is visible, and along with the increase of sample content, the average daily gain of chicken is also along with increase in the daily ration, apparently higher than control group ( P<0.05, P<0.01), and daily ingestion amount does not increase, average daily ingestion amount indifference (P > between each group; 0.05).Daily gain and daily ingestion amount indistinction between each sample.
3. Contain dregs of a decoction fermentation wastes forage feed rabbit
The healthy young rabbit of 30-40 age in days, male and female half and half are selected in experiment for use.Dregs of a decoction fermentation wastes is added in the young rabbit feed diet in 2%, 4%, 6% ratio, and its rabbit daily ration that contains dregs of a decoction fermentation wastes is processed granule, is contrast with the basal diet that does not add fermentate, and 20 every group, the observation period is limited to 50 days.Observe each style to the increase day by day influence of body weight of rabbit.The result sees table 3.
Table 3 is the result show, feeds the rabbit average daily gain contain sample and all is higher than control group, wherein contain high more its better effects if of sample size ( P<0.05), equally to average daily ingestion amount indifference (P > between each group of rabbit; 0.05).Daily gain and daily ingestion amount indistinction between each sample.
Table 3 sample to the influence (g) of young rabbit average daily gain of meat and daily ingestion amount (
Figure 326870DEST_PATH_IMAGE001
± s, n=20)
Figure 456369DEST_PATH_IMAGE004
Compare * with control group P<0.05
The present invention has obtained good effect through animal experimental observation, draws to draw a conclusion:
1. the present invention comprehensively and fully effectively develops the trollflower resource, is used for clinical or veterinary drug as medicine the extract after the fermentation of the trollflower dregs of a decoction; Trollflower dregs of a decoction fermentation wastes with behind the extraction medicinal ingredient prepares additive for farm animal feed, and the environmental pollution that not only can avoid dregs of a decoction discarded object to bring has also solved the high-quality feed shortage problem that limits China's animal husbandry development simultaneously, has bright prospects.
2. trollflower dregs of a decoction fermentation wastes of the present invention prepares feed addictive, can significantly increase the livestock and poultry animal body weight and does not increase food consumption rate and feeding cost, increases economic benefit.

Claims (3)

1. one kind prepares the method for feed addictive with trollflower dregs of a decoction fermentation wastes, it is characterized in that comprising following processing step:
1. the trollflower dregs of a decoction after the drying are ground into powder;
2. the saccharomycete activation and proliferation is cultivated, and the enrichment liquid body of getting behind the autoclaving is cultivated based in the sterile chamber, adds Angel dry ferment 5-10 gram/100ml under the gnotobasis, puts 35-37 ℃ of constant incubator cultivation 5-7 h;
3. the value-added yeast liquid of activation being added the mass and size mark is 40-60% trollflower dregs of a decoction powder, 4% peptone, 4% glucose, mixes the continued fermentation;
4. fermentation condition is 30-37 ℃ of cultivation temperature, incubation time 72-96 hours;
5. after the fermentation ends, fermentate is extracted effective medicinal components with absolute ethyl alcohol, process is centrifugal behind the adding absolute ethyl alcohol, contains medicinal ingredient in the supernatant, is used for extracting the preparation medicine, sinks partly as the feed addictive raw material;
6. with 90 ℃ of dryings of above-mentioned feed addictive raw material, grind into powder, radio sterilization is feed addictive.
2. prepare the method for feed addictive according to said a kind of of claim 1 with trollflower dregs of a decoction fermentation wastes, it is characterized in that described feed addictive processes granule or pulvis, feed addictive is added in the livestock and poultry diet by mass ratio 2%-6%.
3. according to claim 1 or 2 said a kind of methods that prepare feed addictive with trollflower dregs of a decoction fermentation wastes; It is characterized in that describedly getting enrichment liquid body culture medium behind the autoclaving in following ratio: peptone 40 g, yeast soak powder 20 g, glucose 40 g, place 1000 ml distilled water.
CN 201210236188 2012-07-10 2012-07-10 Method for preparing feed additive by fermentation waste of trollflower dregs Expired - Fee Related CN102726603B (en)

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103583807A (en) * 2013-11-10 2014-02-19 河南科技学院 Method of using cordate houttuynia herb residue fermentation material to prepare feed additive
CN104171325A (en) * 2014-07-29 2014-12-03 张释文 Animal feed
CN109418536A (en) * 2017-08-22 2019-03-05 荣昌制药(淄博)有限公司 A kind of Chinese medicine slag production feed and preparation method thereof
NL2031078B1 (en) * 2022-02-24 2023-09-06 Agricultural Biotechnology Res Center Ningxia Academy Of Agricultural And Forestry Sciences Ningxia Feed additive containing trollius chinensis bunge and feed

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101401921A (en) * 2008-10-30 2009-04-08 河南省龙腾高科实业有限公司 Microbial ecological traditional Chinese medicine preparation for livestock and poultry from fermentation production of multiple bacterials and fermentation method thereof
CN102210388A (en) * 2011-07-27 2011-10-12 河北联合大学 Method for preparing feed additive by using buckwheat flower and leaf wastes after flavone extraction

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101401921A (en) * 2008-10-30 2009-04-08 河南省龙腾高科实业有限公司 Microbial ecological traditional Chinese medicine preparation for livestock and poultry from fermentation production of multiple bacterials and fermentation method thereof
CN102210388A (en) * 2011-07-27 2011-10-12 河北联合大学 Method for preparing feed additive by using buckwheat flower and leaf wastes after flavone extraction

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103583807A (en) * 2013-11-10 2014-02-19 河南科技学院 Method of using cordate houttuynia herb residue fermentation material to prepare feed additive
CN104171325A (en) * 2014-07-29 2014-12-03 张释文 Animal feed
CN109418536A (en) * 2017-08-22 2019-03-05 荣昌制药(淄博)有限公司 A kind of Chinese medicine slag production feed and preparation method thereof
NL2031078B1 (en) * 2022-02-24 2023-09-06 Agricultural Biotechnology Res Center Ningxia Academy Of Agricultural And Forestry Sciences Ningxia Feed additive containing trollius chinensis bunge and feed

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