CN103583807A - Method of using cordate houttuynia herb residue fermentation material to prepare feed additive - Google Patents
Method of using cordate houttuynia herb residue fermentation material to prepare feed additive Download PDFInfo
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Abstract
The invention discloses a method of using a cordate houttuynia herb residue fermentation material to prepare a feed additive. The technical scheme of the method is as follows: the method is characterized by comprising the following technical steps: (1) drying the cordate houttuynia herb residue and crushing the dried residue to powder; (2) propagating saccharomycetes: taking an enrichment fluid nutrient medium to be autoclaved, and placing the medium in a sterile container, adding Angel dried yeast according to the dose of 5-10 g per 100 ml in a sterile condition, placing the yeast and the culture medium in a constant temperature incubator at 30-37 DEG C to culture for 5-7 h; (3) adding the following materials in the propagated saccharomycetes liquid by weight volume fraction: for each 100 ml of the propagated saccharomycetes liquid, 30-60 g of cordate houttuynia herb residue powder, 1 g of yeast powder, 2 g of peptone and 2 g of glucose, mixing the materials uniformly and continuously fermenting; (4) keeping the fermentation conditions that the culture temperature is 30-37 DEG C, the pH value is 4.0-6.0, and the culture time is 48-72 h; (5) after fermentation, drying the yeast, grinding the yeast to powder, carrying out radiation sterilization, and finishing preparation. The method is simple in technical process, free from special instrumentation, short in production cycle, low in cost, and suitable for industrial production.
Description
Technical field:
The present invention relates to a kind of method of preparing feed addictive, particularly a kind of method of preparing feed addictive with cordate houttuynia dregs of a decoction fermentate.
Background technology:
Chinese medicine is of long standing and well established in the history of China, is the quintessence of Chinese culture, and in recent years, along with the approval of international community to Chinese medicine, the output of Chinese medicine presents explosive growth, and dregs of a decoction amount also grows with each passing day thereupon.Dregs of a decoction moisture is high, and very easily corrupt, taste is smelly different.The form that Chinese medicine slag is processed at present mainly comprises input dustbin, landfill, burning etc.These processing methods not only cause the wasting of resources, contaminated environment, and time and effort consuming, and how processing Chinese medicine slag has become a problem demanding prompt solution.Therefore, rationally utilize Chinese medicine slag, to reaching, turn waste into wealth, the utilization rate that improves natural resources of Chinese medicinal materials receives everybody concern day by day.In the dregs of a decoction, be rich in the nascent materials such as rich in protein, cellulose, because mostly being decocting, current traditional Chinese medicine extraction boils, in the dregs of a decoction after decocting, also remain part active component, these residual active materials make the dregs of a decoction have very high recycle value, therefore, the dregs of a decoction start to be subject to common people's attention as a kind of resource, become one of important content of modernization of cmm.
Cordate houttuynia, has another name called heartleaf houttuynia, belongs to dicotyledon Saururaceae heartleaf houttuynia and belong on taxology, be a kind of herbaceous plant ， China Yangtze river basin with fishy smell and on the south each province all have wild distribution.Cordate houttuynia has very high medical value and edibility, and its plant strain growth is healthy and strong, resistant to diseases and insects is extremely strong, during cultivation without carrying out insect pest preventing and controlling, can avoid drug contamination, be a kind of free of contamination medicine, dish dual-purpose plant, as the development and utilization of green non-pollution health vegetables, have vast potential for future development.Cordate houttuynia is considered to have effects for removing toxic heat aspect the traditional Chinese medical science, cordate houttuynia can be used for preparing houttuynia cordata injection, desinfactant detergent at present, health-care vinegar etc., it has significantly antibiotic, antiviral, anti-inflammatory, diuresis, has a Promote immunity function, the treatment important in inhibiting to infectious diseases simultaneously.According to incompletely statistics, the dregs of a decoction that the whole nation utilizes cordate houttuynia pharmacy to produce every year have millions of tons, and these dregs of a decoction overwhelming majority is abandoned in vain, and not only contaminated environment, and producer also will consume a large amount of expenses for processing the dregs of a decoction.Therefore how the cordate houttuynia dregs of a decoction are utilized completely, turn waste into wealth, of far-reaching significance.
Summary of the invention:
The object of this invention is to provide a kind of method of preparing feed addictive with cordate houttuynia dregs of a decoction fermentate, its cost is low, effective, and application is convenient, belongs to the recycling of dregs of a decoction discarded object, and not only environmental protection but also economy, can solve again the problem existing in background technology.
Technical scheme of the present invention is that a kind of method of preparing feed addictive with cordate houttuynia dregs of a decoction fermentate, is characterized in that comprising following processing step: 1. by cordate houttuynia dregs of a decoction crushed after being dried powdered; 2. saccharomycetic expansion is cultivated, get increase bacterium with after fluid nutrient medium autoclaving in sterile chamber, asepticly add Angel dry ferment 5-10 gram/100ml, put 30-37 ℃ of constant incubator and cultivate 5-7 h; 3. add bulking value mark to be the yeast liquid of expand cultivating: cordate houttuynia dregs of a decoction powder 30-60 g/100ml, dusty yeast 1 g/100ml, peptone 2 g/100ml, glucose 2 g/100ml, to mix rear continuation fermentation; 4. fermentation condition is cultivation temperature 30-37 ℃, pH4.0-6.0, incubation time 48-72h; 5. after fermentation ends, fermentate is dry, grind into powder, radiation sterilization, completes preparation.The described enrichment liquid body culture medium of getting after autoclaving is prepared in following ratio: potato 200g, glucose 20g put in 1000ml distilled water.The described enrichment liquid body culture medium of getting after autoclaving is prepared in following ratio: yeast extract 10g, and peptone 20g, glucose 20g, puts in 1000ml distilled water.The present invention in use, can add by 2%-6% of the gross weight of feed the feed addictive preparing in livestock and poultry diet to.
The present invention has the following advantages:
(1) raw material resources are abundant: making full use of after discarded cordate houttuynia dregs of a decoction fermentation, meet the industrial policy of sustainable development and recycling economy, poultry industry is benefited huge, have popularizing application prospect, can produce significant economic benefit and social benefit; The enterprise of whole nation application cordate houttuynia pharmacy is a lot, so dregs of a decoction aboundresources, is easy to get, and the recycling that the present invention is cordate houttuynia resource and new purposes provide scientific basis;
(2) this feed addictive is nutritious: because of the cordate houttuynia dregs of a decoction and yeast thalline, have the compositions useful to body such as abundant mineral matter, protein (containing a large amount of mycoproteins), trace element, vitamin, polysaccharide, each seed amino acid and unrighted acid, so it is nutritious as feed addictive to get its fermentate;
(3) preparation technology is simple: this method technological process is simple, does not need specific apparatus and equipment, and with short production cycle, cost is low, is suitable for suitability for industrialized production;
(4) application is convenient, successful: said preparation is solid dosage forms, and consumption is little, potency ratio is high, store and application all facilitates, and is applied in piglet, meat chick, sheep daily ration, and the body weight that increases day by day is apparently higher than control group, and food consumption does not increase;
(5) reduce and pollute, increase economic benefit: cordate houttuynia dregs of a decoction water content is higher, very easily corrupt, can cause serious pollution to environment, and in dregs of a decoction fermentate, still contain protein, polysaccharide, various vitamin and trace element etc. and can utilize composition, in time it is carried out comprehensively, is made full use of, not only can contaminated solution problem, also can bring certain economic benefit; The high-quality feed shortage problem that has also simultaneously solved restriction China animal husbandry development, has broad prospects.
The specific embodiment:
Embodiment mono-:
1. by the cordate houttuynia dregs of a decoction through 85 ℃, 2.5h crushed after being dried;
2. saccharomycete expands cultivation: saccharomycete: enrichment liquid body culture medium=6g:100ml, and 30 ℃ of constant incubators are cultivated 7h;
3. by expanding the yeast liquid of cultivating, add: cordate houttuynia dregs of a decoction powder 50 g/100ml, dusty yeast 1 g/100ml, peptone 2 g/100ml, glucose 2 g/100ml, mix rear continuation fermentation;
4. fermentation condition is 30 ℃ of cultivation temperature, incubation time 72h;
5. fermentate through 80 ℃ dry, grind into powder, radio sterilization, is feed addictive.
Embodiment bis-
1. by the cordate houttuynia dregs of a decoction through 85 ℃, 2h crushed after being dried;
2. saccharomycete expands cultivation: saccharomycete: enrichment liquid body culture medium=7g:100ml, and 37 ℃ of constant incubators are cultivated 5h;
3. by expanding the yeast liquid of cultivating, add: cordate houttuynia dregs of a decoction powder 40 g/100ml, dusty yeast 1 g/100ml, peptone 2 g/100ml, glucose 2 g/100ml, mix rear continuation fermentation;
4. fermentation condition is 33 ℃ of cultivation temperature, incubation time 72h;
5. fermentate through 85 ℃ dry, grind into powder, radio sterilization, is feed addictive.
Embodiment tri-
1. by the cordate houttuynia dregs of a decoction through 85 ℃, 3h crushed after being dried;
2. saccharomycete expands cultivation: saccharomycete: enrichment liquid body culture medium=10g:100ml, and 34 ℃ of constant incubators are cultivated 6h;
3. by expanding the yeast liquid of cultivating, add: cordate houttuynia dregs of a decoction powder 60 g/100ml, dusty yeast 1 g/100ml, peptone 2 g/100ml, glucose 2 g/100ml, mix rear continuation fermentation;
4. fermentation condition is 37 ℃ of cultivation temperature, incubation time 60h;
5. fermentate through 90 ℃ dry, grind into powder, radio sterilization, is feed addictive.
Embodiment tetra-
1. by the cordate houttuynia dregs of a decoction through 85 ℃, 3h crushed after being dried;
2. saccharomycete expands cultivation: saccharomycete: enrichment liquid body culture medium=10g:100ml, and 34 ℃ of constant incubators are cultivated 6h;
3. by expanding the yeast liquid of cultivating, add: cordate houttuynia dregs of a decoction powder 60 g/100ml, potato 200g, glucose 20g put in 1000ml distilled water, mix rear continuation fermentation;
4. fermentation condition is 37 ℃ of cultivation temperature, incubation time 60h;
5. fermentate through 90 ℃ dry, grind into powder, radio sterilization, is feed addictive.
Feed addictive by embodiment mono-, two, three preparations, adds in Fattening Sheep, meat chick and piglet diet by 2%, 4%, 6% respectively, carries out zoopery.
1. cordate houttuynia dregs of a decoction fermentate feed is fed Fattening Sheep test.
Select hereditary basis similar, be in a good state of health, average weight is 40 ± 2kg (polled doset * little tail tremble with fear sheep) F2 is for 40 of hybrid Fattening Sheeps, is divided at random 4 groups, raising term harmonization.Cordate houttuynia dregs of a decoction fermentate is added in Fattening Sheep concentrated feed daily ration in 2%, 4%, 6% ratio, it is containing the Fattening Sheep daily ration granulation agent of dregs of a decoction fermentate, the basal diet that does not add fermentate of take is contrast, observation period is limited to 50 days, when starting and finishing respectively at experiment, weigh (morning, fixing a point to weigh on an empty stomach), calculate average daily gain situation and average fine fodder daily ingestion amount.The results are shown in Table 1 and table 2.
Table 1 cordate houttuynia dregs of a decoction fermentate on the impact of Fattening Sheep average daily gain (g) (
± s, n=10)
| Sample | Test 1 group (2%) | Test 2 groups (4%) | Test 3 groups (6%) | Control group |
| Embodiment mono- | 212.00 a±45.00 | 234.00 b±36.00 | 241.00 c±36.00 | 200.00 a±35.00 |
| Embodiment bis- | 209.00 a±36.00 | 231.00 b±39.00 | 238.00 c±39.00 | 198.00 a±42.00 |
| Embodiment tri- | 225.00 a±42.00 | 237.00 b±31.00 | 247.00 c±31.00 | 205.00 a±32.00 |
Note: colleague's data shoulder motes letter identical table differential different not remarkable (P > 0. 05), neighboar lists shows significant difference (P < 0. 05), and alternate expression difference is (P < 0. 01) extremely significantly.Following table is same
Table 1 result shows, increase along with addition, Fattening Sheep daily gain value also increases, test 2 groups with test 3 groups of weight averages that increase day by day apparently higher than control group (P < 0.05, P < 0.01), testing the average daily gain of 1 group compares with control group and increases but no significant difference (P>0.05).
Table 2 cordate houttuynia dregs of a decoction fermentate on the impact of the average daily ingestion amount of Fattening Sheep concentrated feed (g) (
± s, n=10)
| Sample | Test 1 group (2%) | Test 2 groups (4%) | Test 3 groups (6%) | Control group |
| Embodiment mono- | 622.00 a±25.00 | 634.00 a±31.00 | 624.00 a±26.00 | 620.00 a±45.00 |
| Embodiment bis- | 609.00 a±32.00 | 631.00 a±42.00 | 618.00 a±49.00 | 615.00 a±61.00 |
| Embodiment tri- | 625.00 a±37.00 | 617.00 a±61.00 | 627.00 a±38.00 | 625.00 a±53.00 |
The demonstration of table 2 result, along with the increase of addition, the average daily ingestion amount of the concentrated feed of test group is compared no significant difference (P>0.05) with control group.
2. cordate houttuynia dregs of a decoction fermentate feed is fed the test of meat chick.
15 Day-old Broiler Chickens, male and female half and half are selected in experiment.Cordate houttuynia fermentate is added in chicken feed diet in 2%, 4%, 6% ratio, and it makes pulvis containing broiler diet of dregs of a decoction fermentate, and the basal diet that does not add dregs of a decoction fermentate of take is contrast, and 20 every group, the observation period is limited to 30 days.When starting and finishing respectively at experiment, weigh (morning, fixing a point to weigh on an empty stomach), calculate average daily gain situation.The results are shown in Table 3.
Table 3 cordate houttuynia dregs of a decoction fermentate on the impact of meat chick average daily gain (g) (
± s, n=20)
| Sample | Test 1 group (2%) | Test 2 groups (4%) | Test 3 groups (6%) | Control group |
| Embodiment mono- | 71.62 b±5.93? | 75.69 c±5.85 | 76.41 c±5.76 | 60.10 a±4.84 |
| Embodiment bis- | 72.84 b±6.06 | 76.71 c±5.63 | 77.37 c±5.93 | 61.21 a±4.59 |
| Embodiment tri- | 70.49 b±5.93 | 76.05 c±5.74 | 76.19 c±5.72 | 59.82 a±5.68 |
The demonstration of table 3 result, along with the increase of addition, broiler chicken daily gain value also increases, and tests each group and increases day by day weight average apparently higher than control group (P < 0.05, P < 0.01).
3. cordate houttuynia dregs of a decoction fermentate feed is fed piglets
40 piglets that selection hereditary basis is similar, sex consistent, be in a good state of health, average weight is 10kg, are divided into 4 groups at random, raise term harmonization.Dregs of a decoction fermentate is added in the conventional daily ration of piglet in 2%, 4%, 6% ratio, feed 35 days, establish control group simultaneously, feed conventional daily ration.When starting and finishing respectively at experiment, weigh (morning, fixing a point to weigh on an empty stomach), calculate average daily gain situation.The results are shown in Table 4.
Table 4 cordate houttuynia dregs of a decoction fermentate on the impact of piglet average daily gain (g) (
± s, n=10)
| Sample | Test 1 group (2%) | Test 2 groups (4%) | Test 3 groups (6%) | Control group |
| Embodiment mono- | 492.00 b±58.00 | 535.00 c±56.00 | 539.00 c±47.00 | 440.10 a±48.00 |
| Embodiment bis- | 489.00 b±47.00 | 527.00 c±49.00 | 542.00 c±53.00 | 439.00 a±45.00 |
| Embodiment tri- | 497.00 b±53.00 | 537.00 c±51.00 | 538.00 c±55.00 | 442.00 a±56.00 |
The demonstration of table 3 result, along with the increase of addition, piglet daily gain value also increases, and tests each group and increases day by day weight average apparently higher than control group (P < 0.05, P < 0.01).
The present invention observes by animal experiment, has obtained very good effect, draws to draw a conclusion:
1. the present invention comprehensively and fully effectively develops cordate houttuynia resource, with the cordate houttuynia dregs of a decoction fermentate extracting after medicinal ingredient, prepare additive for farm animal feed, the environmental pollution bringing after not only can avoiding the dregs of a decoction discarded, the high-quality feed shortage problem that has also simultaneously solved restriction China animal husbandry development, has broad prospect of application.
2. cordate houttuynia dregs of a decoction fermentate of the present invention is prepared feed addictive, is not adding under the prerequisite of antibiotic and hormone, can significantly increase livestock and poultry animal body weight and not increase feeding cost, has improved animal products quality, has increased economic benefit.
Claims (3)
1. a method of preparing feed addictive with cordate houttuynia dregs of a decoction fermentate, it is characterized in that: a kind of method of preparing feed addictive with cordate houttuynia dregs of a decoction fermentate, is characterized in that comprising following processing step: 1. by cordate houttuynia dregs of a decoction crushed after being dried powdered; 2. saccharomycetic expansion is cultivated, get increase bacterium with after fluid nutrient medium autoclaving in sterile chamber, asepticly add Angel dry ferment 5-10 gram/100ml, put 30-37 ℃ of constant incubator and cultivate 5-7 h; 3. add bulking value mark to be the yeast liquid of expand cultivating: cordate houttuynia dregs of a decoction powder 30-60 g/100ml, dusty yeast 1 g/100ml, peptone 2 g/100ml, glucose 2 g/100ml, to mix rear continuation fermentation; 4. fermentation condition is cultivation temperature 30-37 ℃, pH 4.0-6.0, incubation time 48-72h; 5. after fermentation ends, fermentate is dry, grind into powder, radiation sterilization, completes preparation.
2. a kind of method of preparing feed addictive with cordate houttuynia dregs of a decoction fermentation wastes as claimed in claim 1, is characterized in that: the described enrichment liquid body culture medium of getting after autoclaving is prepared in following ratio: potato 200g, glucose 20g put in 1000ml distilled water.
3. a kind of method of preparing feed addictive with cordate houttuynia dregs of a decoction fermentation wastes as claimed in claim 1, it is characterized in that: the described enrichment liquid body culture medium of getting after autoclaving is prepared in following ratio: yeast extract 10g, peptone 20g, glucose 20g, puts in 1000ml distilled water.
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101861953A (en) * | 2010-04-23 | 2010-10-20 | 韦东吉 | Feed Chinese medical herb composition, feed additive, feed and preparation method |
| CN102726603A (en) * | 2012-07-10 | 2012-10-17 | 河北联合大学 | Method for preparing feed additive by fermentation waste of trollflower dregs |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101861953A (en) * | 2010-04-23 | 2010-10-20 | 韦东吉 | Feed Chinese medical herb composition, feed additive, feed and preparation method |
| CN102726603A (en) * | 2012-07-10 | 2012-10-17 | 河北联合大学 | Method for preparing feed additive by fermentation waste of trollflower dregs |
Non-Patent Citations (2)
| Title |
|---|
| 李艳军 等: "中药渣饲料资源的开发利用", 《饲料博览》 * |
| 王兵 等: "中药渣固态发酵生产蛋白饲料", 《食品与生物技术学报》 * |
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Application publication date: 20140219 |