CN106720936A - A kind of fermented feed additive containing dalbergia wood leaf composition and its preparation method and application - Google Patents

A kind of fermented feed additive containing dalbergia wood leaf composition and its preparation method and application Download PDF

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Publication number
CN106720936A
CN106720936A CN201710049129.5A CN201710049129A CN106720936A CN 106720936 A CN106720936 A CN 106720936A CN 201710049129 A CN201710049129 A CN 201710049129A CN 106720936 A CN106720936 A CN 106720936A
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parts
dalbergia wood
leaf
feed additive
raw material
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CN106720936B (en
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孟慧
杨云
陈波
黄良明
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Hainan Runsen technology research and Development Co.,Ltd.
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Institute of Medicinal Plant Development of CAMS and PUMC
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Abstract

The invention provides a kind of fermented feed additive containing dalbergia wood leaf composition and its preparation method and application.The raw material of the fermented feed additive containing dalbergia wood leaf composition provided by the present invention, including herbal raw material, water and composite ferment;The sufficient raw of the fermented feed additive containing dalbergia wood leaf composition provided by the present invention, cheap, low cost;The abilities of digestive and absorption of poultry is effectively improved, feedstuff-meat ratio is reduced, reduces cost improves cultivation value;Immunity of poultry can also be improved simultaneously, the incidence of disease of poultry is significantly reduced, the use of antibiotic etc is reduced, is conducive to food security.The preparation method of the fermented feed additive containing dalbergia wood leaf composition provided by the present invention is that Chinese herbal medicine is fermented using biofermentation technique, the advantage of " amount is less, efficiently, safely " can be played on husbandry sector, develop nuisanceless animal husbandry, production safe animal products, the competitiveness in the international market of enhancing China livestock products.

Description

A kind of fermented feed additive containing dalbergia wood leaf composition and its preparation method and application
Technical field
The invention belongs to field of feed additive technology, and in particular to a kind of fermented type feed addition containing dalbergia wood leaf composition Agent and its preparation method and application.
Background technology
In recent years, the intestines problem of poultry increased increasingly, has had a strong impact on the speed of growth of poultry, thus has been badly in need of raising and has exempted from The somatotrophic product of epidemic disease power.Noted using increasingly inducing one in feedstuff industry in Chinese herbal medicine combination and solid microbe fermentation technology Mesh.Although biological solid-state fermentation technology, using relatively broad, in the prior art, is entered using single culture more in feedstuff industry Row fermentation prepares feed addictive, low yield and effect is undesirable.
In terms of Chinese herbal medicine, dalbergia wood heartwood has been reported for feedstuff industry.Dalbergia wood is legume dalbergia wood wingceltis The dry duramen of (Dalbergia odorifera T.Chen) trunk and root, Hainan is peculiar, is Chinese Second Class Key Protected Plant, is A kind of traditional rare traditional Chinese medicine and rare spice, the medicinal ingredient contained by it have economic worth very high, with recent year Outer research deepens continuously, and its chemical composition and pharmacological action be constantly clear.But, the own warp that uses of heartwood is caused for a long time The dalbergia wood utilization of resources is nervous, and dalbergia wood leaf is only intended to food addition and general spices is used, and is not utilized sufficiently Get up, be not acquired generally, cause the waste of resource, do not there is the deep processing to dalbergia wood leaf particularly, make its more preferable performance Its healthcare function and its proper value.According to the literature, the main component of dalbergia wood leaf volatile oil should belong to palmitic acid by chemical classification Class and phytic acid class;And the main component of the volatile oil of dalbergia wood heartwood is nerolidol (nerolidol), dalbergia wood leaf volatile oil and its Chemical composition contained by heartwood is different, therefore its pharmacological action is likely to difference, in the Chinese herbal medicine combination for feedstuff industry, it is impossible to Simply dalbergia wood heartwood will be replaced with dalbergia wood leaf, and, not yet have is used for the report of poultry feed using dalbergia wood blade at present.
Therefore, it is necessary to develop a kind of Composite fermentation type feed addictive containing dalbergia wood leaf composition.
The content of the invention
The present invention is to make up the deficiencies in the prior art, there is provided a kind of fermented feed additive containing dalbergia wood leaf composition and Its preparation method and application.
In a first aspect, the invention provides a kind of fermented feed additive containing dalbergia wood leaf composition, the leaf containing dalbergia wood The raw material of the fermented feed additive of composition, including herbal raw material, water and composite ferment, wherein, the composite fermentation The weight of agent is 0.02~0.1 times of the herbal raw material weight;The weight of the water is the herbal raw material weight 0.4~1 times;
The herbal raw material, counts by weight, including:40~60 parts of dalbergia wood leaf, 35~50 parts of fly maggot larva, punching 3~10 parts of lotus, 2~10 parts of Coleus forskohlii, 2~8 parts of broomcorn straw, 5~10 parts of balloonflower root, 3~12 parts of fructus cannabis, fructus alpiniae oxyphyllae 3 ~12 parts, 3~12 parts of hot pepper leaf, 5~10 parts in one's early teens, 1~6 part of the Fen, Amorphophalus rivieris of acer truncatum pollen 2~10, thick wood-fern rhizome 1~6 Part, 5~10 parts of duckweed, 2~8 parts of Fructus Aurantii, 3~7 parts of loguat leaf, 1~5 part of beautiful mapler leaf;
The composite ferment includes but is not limited to the one kind or several in mould, head mold, bacillus subtilis, aspergillus niger Kind.
Preferably, the weight of the composite ferment is 0.04~0.08 times of the herbal raw material weight.
Preferably, the composite ferment includes mould and head mold, and the mould is 1 with the weight ratio of head mold:1~5.
It is further preferred that in the composite ferment, the mould is 1 with the weight ratio of head mold:2~5.
Preferably, the weight of the water is 0.4~0.8 times of the herbal raw material weight.
Preferably, the herbal raw material, counts by weight, including:50~60 parts of dalbergia wood leaf, fly maggot larva 35~45 Part, 3~6 parts of Herba Andrographitis, 2~8 parts of Coleus forskohlii, 2~5 parts of broomcorn straw, 5~8 parts of balloonflower root, 3~8 parts of fructus cannabis, intelligence development 3~8 parts of benevolence, 3~8 parts of hot pepper leaf, 5~10 parts in one's early teens, 2~5 parts of the Fen, Amorphophalus rivieris of acer truncatum pollen 2~7, thick wood-fern rhizome 2~5 Part, 5~10 parts of duckweed, 2~5 parts of Fructus Aurantii, 3~7 parts of loguat leaf, 2~4 parts of beautiful mapler leaf.
Second aspect, the invention provides a kind of preparation method of the fermented feed additive containing dalbergia wood leaf composition, bag Include following steps:
(1) herbal raw material of following portions by weight is taken:40~60 parts of dalbergia wood leaf, 35~50 parts of fly maggot larva, Herba Andrographitis 3 ~10 parts, 2~10 parts of Coleus forskohlii, 2~8 parts of broomcorn straw, 5~10 parts of balloonflower root, 3~12 parts of fructus cannabis, fructus alpiniae oxyphyllae 3~12 Part, 3~12 parts of hot pepper leaf, 5~10 parts in one's early teens, it is 1~6 part of the Fen, Amorphophalus rivieris of acer truncatum pollen 2~10,1~6 part of thick wood-fern rhizome, floating 5~10 parts of duckweed, 2~8 parts of Fructus Aurantii, 3~7 parts of loguat leaf, 1~5 part of beautiful mapler leaf, co-grinding are obtained Chinese herbal medicine powder;
(2) it is 0.4~1 according to the mass ratio of water and the herbal raw material:1 ratio, by obtained by water and step (1) Defibrination after the Chinese herbal medicine powder mixing, is obtained slurries;
(3) it is 0.02~0.1 according to the mass ratio of composite ferment and the herbal raw material:1 ratio, will be described multiple Close leavening to mix with the slurries obtained by step (2), fermented 3~8 days under conditions of 22~30 DEG C, be obtained through low temperature drying and raised Feed additives, the composite ferment includes but is not limited to the one kind or several in mould, head mold, bacillus subtilis, aspergillus niger Kind.
Preferably, in the step (1), the size of the Chinese herbal medicine powder is 20~50 mesh.
Preferably, in the step (1), the herbal raw material is counted by weight, including:50~60 parts of dalbergia wood leaf, 35~45 parts of fly maggot larva, 3~6 parts of Herba Andrographitis, 2~8 parts of Coleus forskohlii, 2~5 parts of broomcorn straw, 5~8 parts of balloonflower root, fire fiber crops 3~8 parts of benevolence, 3~8 parts of fructus alpiniae oxyphyllae, 3~8 parts of hot pepper leaf, 5~10 parts in one's early teens, 2~5 parts of the Fen, Amorphophalus rivieris of acer truncatum pollen 2~7, 2~5 parts of thick wood-fern rhizome, 5~10 parts of duckweed, 2~5 parts of Fructus Aurantii, 3~7 parts of loguat leaf, 2~4 parts of beautiful mapler leaf.
Preferably, in the step (2), the weight of the water is 0.4~0.8 times of the herbal raw material weight.
Preferably, in the step (3), the weight of the composite ferment be the herbal raw material weight 0.04~ 0.08 times.
Preferably, in the step (3), the weight of the composite ferment be the herbal raw material weight 0.04~ 0.08 times.
Preferably, in the step (3), the composite ferment includes the weight of mould and head mold, the mould and head mold Amount is than being 1:1~5.
It is further preferred that in the composite ferment, the mould is 1 with the weight ratio of head mold:2~5.
It is understood that the present invention does not have special limitation to the method for the low temperature drying described in the step (3), adopt With the technical scheme of low temperature drying well known to those skilled in the art.In the step (3), the temperature of the low temperature drying Spend is 30~50 DEG C.
Preferably, in the step (3), the water content of the feed addictive is not higher than 15%.
Preferably, the fermented feed additive containing dalbergia wood leaf composition described in first aspect present invention is using such as second A kind of preparation method of the fermented feed additive containing dalbergia wood leaf composition described in aspect is obtained.
The third aspect, present invention also offers a kind of by the fermented type feed containing dalbergia wood leaf composition as described in relation to the first aspect Application of the additive in animal and fowl fodder is prepared.
Preferably, the addition of the fermented feed additive containing dalbergia wood leaf composition as described in relation to the first aspect is the poultry 0.05~1% (more preferably 0.05~0.1%) of fowl feed gross weight.
Compared with prior art, the present invention has the advantages that:
(1) sufficient raw of the fermented feed additive containing dalbergia wood leaf composition in the present invention, cheap, into This is low;
(2) fermented feed additive containing dalbergia wood leaf composition provided by the present invention effectively improves digesting and assimilating for poultry Ability, reduces feedstuff-meat ratio, and reduces cost improves cultivation value;Immunity of poultry can also be improved simultaneously, the hair of poultry is significantly reduced Sick rate, reduces the use of antibiotic etc, is conducive to food security.
(3) preparation method of the fermented feed additive containing dalbergia wood leaf composition provided by the present invention is sent out using biological Ferment technology is fermented to Chinese herbal medicine, on the one hand, can not only strengthen the pharmacological activity of Chinese herbal medicine, strengthens its effect, also in fermentation Introduced in Chinese herbal medicine Institute of Micro-biology generation metabolite, the microorganism for containing simultaneously can also regulating intestinal canal flora, be conducive to The healthy growth of raw poultry, expansion uses scope;On the other hand, microorganism producing enzyme can help animal digestion feed, promote life It is long, improve culture benefit;
In a word, the advantage of " amount is less, efficiently, safely " can be played on husbandry sector by fermentation of Chinese herbal medicine, is developed without public affairs Evil animal husbandry, produces safe animal products, the competitiveness in the international market of enhancing China livestock products.
Specific embodiment
The present invention is further described with reference to specific embodiment, but not limitation of the present invention, it is all according to this hair The equivalent of any this area that bright content is done, belongs to protection scope of the present invention.
Embodiment 1
The invention provides a kind of preparation method of the fermented feed additive containing dalbergia wood leaf composition, including following step Suddenly:
(1) herbal raw material of following portions by weight is taken:56 parts of dalbergia wood leaf, 38 parts of fly maggot larva, Herba Andrographitis 3 part, mao larynx Sheath stamen spend 2 parts, 2 parts of broomcorn straw, 5 parts of balloonflower root, 3 parts of fructus cannabis, 3 parts of fructus alpiniae oxyphyllae, 3 parts of hot pepper leaf, 5 parts in one's early teens, acer truncatum 2 parts of pollen, 2 parts of Amorphophalus rivieri, 2 parts of thick wood-fern rhizome, 5 parts of duckweed, 2 parts of Fructus Aurantii, 3 parts of loguat leaf, 2 parts of beautiful mapler leaf, co-grinding is extremely 40 mesh, are obtained Chinese herbal medicine powder one;
(2) defibrination after 110 parts of water are mixed with the Chinese herbal medicine powder one obtained by step (1), is obtained slurries one;
(3) the Mixed Microbes bacterium powder being made up of 1 part of mould, 2 parts of head molds is mixed with the slurries one obtained by step (2), 25 Fermented 5 days under conditions of DEG C, 10% is not higher than through forced air drying to the water content at 30 DEG C, crush and cross 60 mesh sieves, obtain final product feeding Feed additives one.
Embodiment 2
The invention provides a kind of preparation method of the fermented feed additive containing dalbergia wood leaf composition, including following step Suddenly:
(1) herbal raw material of following portions by weight is taken:60 parts of dalbergia wood leaf, 35 parts of fly maggot larva, Herba Andrographitis 6 part, mao larynx Sheath stamen spend 5 parts, 5 parts of broomcorn straw, 6 parts of balloonflower root, 5 parts of fructus cannabis, 5 parts of fructus alpiniae oxyphyllae, 5 parts of hot pepper leaf, 7 parts in one's early teens, acer truncatum 5 parts of pollen, 5 parts of Amorphophalus rivieri, 4 parts of thick wood-fern rhizome, 7 parts of duckweed, 4 parts of Fructus Aurantii, 5 parts of loguat leaf, 4 parts of beautiful mapler leaf, co-grinding is extremely 30 mesh, are obtained Chinese herbal medicine powder two;
(2) defibrination after 100 parts of water are mixed with the Chinese herbal medicine powder two obtained by step (1), is obtained slurries two;
(3) the Mixed Microbes bacterium powder being made up of 2 parts of moulds, 4 parts of head molds is mixed with the slurries two obtained by step (2), 22 Fermented 7 days under conditions of DEG C, 12% is not higher than through forced air drying to the water content at 30 DEG C, crush and cross 60 mesh sieves, obtain final product feeding Feed additives two.
Embodiment 3
The invention provides a kind of preparation method of the fermented feed additive containing dalbergia wood leaf composition, including following step Suddenly:
(1) herbal raw material of following portions by weight is taken:55 parts of dalbergia wood leaf, 40 parts of fly maggot larva, Herba Andrographitis 5 part, mao larynx Sheath stamen spend 7 parts, 3 parts of broomcorn straw, 8 parts of balloonflower root, 8 parts of fructus cannabis, 8 parts of fructus alpiniae oxyphyllae, 8 parts of hot pepper leaf, 10 parts in one's early teens, acer truncatum 7 parts of pollen, 3 parts of Amorphophalus rivieri, 3 parts of thick wood-fern rhizome, 10 parts of duckweed, 3 parts of Fructus Aurantii, 7 parts of loguat leaf, 4 parts of beautiful mapler leaf, co-grinding is extremely 30 mesh, are obtained Chinese herbal medicine powder three;
(2) defibrination after 130 parts of water are mixed with the Chinese herbal medicine powder three obtained by step (1), is obtained slurries three;
(3) the Mixed Microbes bacterium powder being made up of 1 part of mould, 5 parts of head molds is mixed with the slurries three obtained by step (2), 25 Fermented 8 days under conditions of DEG C, 8% is not higher than through forced air drying to the water content at 30 DEG C, crush and cross 60 mesh sieves, obtain final product feed Additive three.
Embodiment 4
The invention provides a kind of preparation method of the fermented feed additive containing dalbergia wood leaf composition, including following step Suddenly:
(1) herbal raw material of following portions by weight is taken:50 parts of dalbergia wood leaf, 42 parts of fly maggot larva, Herba Andrographitis 4 part, mao larynx Sheath stamen spend 6 parts, 4 parts of broomcorn straw, 6 parts of balloonflower root, 5 parts of fructus cannabis, 5 parts of fructus alpiniae oxyphyllae, 5 parts of hot pepper leaf, 7 parts in one's early teens, acer truncatum 5 parts of pollen, 4 parts of Amorphophalus rivieri, 4 parts of thick wood-fern rhizome, 7 parts of duckweed, 4 parts of Fructus Aurantii, 5 parts of loguat leaf, 2 parts of beautiful mapler leaf, co-grinding is extremely 40 mesh, are obtained Chinese herbal medicine powder four;
(2) defibrination after 130 parts of water are mixed with the Chinese herbal medicine powder four obtained by step (1), is obtained slurries four;
(3) the Mixed Microbes bacterium powder being made up of 1 part of mould, 4.5 parts of head molds is mixed with the slurries four obtained by step (2), Fermented 5 days under conditions of 27 DEG C, 12% is not higher than through forced air drying to the water content at 30 DEG C, crush and cross 60 mesh sieves, high temperature Sterilizing, thus obtaining the product feed addictive four.
Embodiment 5
The invention provides a kind of preparation method of the fermented feed additive containing dalbergia wood leaf composition, including following step Suddenly:
(1) herbal raw material of following portions by weight is taken:52 parts of dalbergia wood leaf, 45 parts of fly maggot larva, Herba Andrographitis 3 part, mao larynx Sheath stamen spend 8 parts, 5 parts of broomcorn straw, 5 parts of balloonflower root, 4 parts of fructus cannabis, 4 parts of fructus alpiniae oxyphyllae, 4 parts of hot pepper leaf, 5 parts in one's early teens, acer truncatum 7 parts of pollen, 2 parts of Amorphophalus rivieri, 2 parts of thick wood-fern rhizome, 5 parts of duckweed, 2 parts of Fructus Aurantii, 4 parts of loguat leaf, 4 parts of beautiful mapler leaf, co-grinding is extremely 30 mesh, are obtained Chinese herbal medicine powder five;
(2) defibrination after 95 parts of water are mixed with the Chinese herbal medicine powder five obtained by step (1), is obtained slurries five;
(3) the Mixed Microbes bacterium powder being made up of 2 parts of moulds, 6 parts of head molds is mixed with the slurries five obtained by step (2), 22 Fermented 8 days under conditions of DEG C, 15% is not higher than through forced air drying to the water content at 50 DEG C, crushed and cross 60 mesh sieves, high temperature goes out Bacterium obtains final product feed addictive five.
Embodiment 6
The invention provides a kind of preparation method of the fermented feed additive containing dalbergia wood leaf composition, including following step Suddenly:
(1) herbal raw material of following portions by weight is taken:60 parts of dalbergia wood leaf, 42 parts of fly maggot larva, Herba Andrographitis 5 part, mao larynx Sheath stamen spend 6 parts, 2 parts of broomcorn straw, 8 parts of balloonflower root, 8 parts of fructus cannabis, 8 parts of fructus alpiniae oxyphyllae, 8 parts of hot pepper leaf, 10 parts in one's early teens, acer truncatum 2 parts of pollen, 5 parts of Amorphophalus rivieri, 5 parts of thick wood-fern rhizome, 10 parts of duckweed, 5 parts of Fructus Aurantii, 7 parts of loguat leaf, 2 parts of beautiful mapler leaf, co-grinding is extremely 40 mesh, are obtained Chinese herbal medicine powder six;
(2) defibrination after 80 parts of water are mixed with the Chinese herbal medicine powder six obtained by step (1), is obtained slurries six;
(3) the Mixed Microbes bacterium powder being made up of 1 part of mould, 5 parts of head molds is mixed with the slurries six obtained by step (2), 22 Fermented 8 days under conditions of DEG C, 12% is not higher than through forced air drying to the water content at 50 DEG C, crushed and cross 60 mesh sieves, high temperature goes out Bacterium obtains final product feed addictive six.
Embodiment 7
The invention provides a kind of preparation method of the fermented feed additive containing dalbergia wood leaf composition, including following step Suddenly:
(1) herbal raw material of following portions by weight is taken:56 parts of dalbergia wood leaf, 38 parts of fly maggot larva, Herba Andrographitis 4 part, mao larynx Sheath stamen spend 5 parts, 4 parts of broomcorn straw, 5 parts of balloonflower root, 5 parts of fructus cannabis, 5 parts of fructus alpiniae oxyphyllae, 5 parts of hot pepper leaf, 7 parts in one's early teens, acer truncatum 5 parts of pollen, 4 parts of Amorphophalus rivieri, 4 parts of thick wood-fern rhizome, 7 parts of duckweed, 4 parts of Fructus Aurantii, 5 parts of loguat leaf, 4 parts of beautiful mapler leaf, co-grinding is extremely 40 mesh, are obtained Chinese herbal medicine powder seven;
(2) defibrination after 100 parts of water are mixed with the Chinese herbal medicine powder seven obtained by step (1), is obtained slurries seven;
(3) the Mixed Microbes bacterium powder being made up of 1.5 parts of moulds, 4.5 parts of head molds is mixed with the slurries seven obtained by step (2), Fermented 7 days under conditions of 23 DEG C, 12% is not higher than through forced air drying to the water content at 40 DEG C, crush and cross 60 mesh sieves, it is high Warm sterilizing, thus obtaining the product feed addictive seven.
Comparative example 1
In order to further illustrate beneficial effects of the present invention, the step of repeat embodiment 3, the step of by embodiment 3 in (3) " 1 part of mould, 5 parts of head molds " be substituted for " 1 part of mould, 5 parts of bacillus subtilises ", be obtained feed addictive eight.
Comparative example 2
In order to further illustrate beneficial effects of the present invention, the step of repeat embodiment 3, the step of by embodiment 3 in (3) " 1 part of mould, 5 parts of head molds " be substituted for " 1 part of bacillus subtilis, 5 parts of head molds ", be obtained feed addictive nine.
Comparative example 3
In order to further illustrate beneficial effects of the present invention, the step of repeat embodiment 6, the step of by embodiment 6 in (1) " 1 part of mould, 5 parts of head molds " be substituted for " 1 part of aspergillus niger, 5 parts of bacillus subtilises ", be obtained feed addictive ten.
Comparative example 4
In order to further illustrate beneficial effects of the present invention, the step of repeat embodiment 7, the step of by embodiment 7 in (1) " 56 parts of dalbergia wood leaf " be substituted for " 56 parts of dalbergia wood heartwood ", be obtained feed addictive 11.
Each 50g of the dry sample of Chinese herbal medicine powder one and feed addictive is taken, following method is respectively adopted carries out the inspection of active ingredient Survey:
Using Water distillation extractor, the interior standard specimen ethyl caprate 0.1ml that extractant adds 200mg/L, continuous steaming are with ether Evaporate extraction 45min, the VFA in feed addictive is fully extracted concentration by ether, collection extract, plus 5g without Aqueous sodium persulfate dehydrates 12h in refrigerator at being statically placed in 4 DEG C, then filters, and Rotary Evaporators vacuumize steaming at 45 DEG C in filtrate After hair ether solvent, 0.1~0.2ml is concentrated into, obtains pale yellow oily liquid, sealing refrigeration treats that GC-MS analyses are used.
GC conditions:Japanese Shimadzu gas chromatography-mass spectrograph band digital display processor, DB-5MS quartz capillary columns (29m × 0.25mm, 0.25 μm).Temperature programming:50 DEG C of column temperature, 8 DEG C/min of heating rate, to 150 DEG C of holding 2.00min;Rise 5 DEG C/min of warm speed, to 180 DEG C of holding 2.00min;To 230 DEG C of holding 7.50min;3 DEG C/min of heating rate, to 280 DEG C of guarantors Hold 5.00min;220 DEG C of injector temperature, 280 DEG C of interface temperature does not shunt (pulse) sample introduction, pulse 50kPa, carrier gas N2, flow velocity 0.8ml/min, detector FID.
Mass Spectrometry Conditions:Bombardment electronic capability:70eV, electron source ionization mode:EI, 200 DEG C of ion source temperature, level Four bar temperature 50 DEG C of degree, electron multiplier voltage:Low 50V, sample size under automatic tuning:2 μ l, sweep limits:20~500amu, solvent retardation Time:3.00min, scan mode:Full scan (scan).
Relative amount of the n- palmitic acid compositions of each sample in volatile oil is obtained (such as by GC-MS analytical calculations Shown in table 1).
As shown in Table 1, the n- palmitic acids in the Chinese herbal medicine powder one and feed addictive one in embodiment 1 contain in percentage Occurs larger difference in amount, the n- palmitic acid compositions of the feed addictive of fermented acquisition have to be increased by a relatively large margin, this and micro- life Hyther in thing secondary metabolism in itself and fermentation process is relevant.
Compared with the content of the n- palmitic acid compositions in the feed addictive obtained by embodiment 1~7, comparative example 8~10 The n- palmitic acid comparison of ingredients of obtained feed addictive is low, this mainly due to dalbergia wood leaf essential oil to bacillus subtilis and Aspergillus niger has preferable inhibitory activity so that the n- palmitic acid comparison of ingredients of the feed addictive obtained by comparative example 8~10 It is low.
The n- palmitic acid compositions of the feed addictive of the gained of comparative example 11 are minimum, used mainly due to comparative example 11 The n- palmitic acid contents of dalbergia wood heartwood are extremely low, and the n- palmitic acids of its fermentation process production are extremely limited.
Relative amount of the n- palmitic acid compositions of table 1 in volatile oil
Embodiment Feed addictive Relative amount
Embodiment 1 Chinese herbal medicine powder one 13.65%
Embodiment 1 Feed addictive one 26.72%
Embodiment 2 Feed addictive two 23.65%
Embodiment 3 Feed addictive three 24.21%
Embodiment 4 Feed addictive four 23.23%
Embodiment 5 Feed addictive five 25.16%
Embodiment 6 Feed addictive six 24.26%
Embodiment 7 Feed addictive seven 26.12%
Comparative example 8 Feed addictive eight 18.26%
Comparative example 9 Feed addictive nine 19.32%
Comparative example 10 Feed addictive ten 15.62%
Comparative example 11 Feed addictive 11 2.20%
Effect example
Designed using single-factor random experiment, 1 aa broiler chicken is divided at random according to the consistent principle of male and female half and half, body weight It is 14 groups, every group 150, wherein, 13 groups of test group, 1 group of control group.It is total according to feed based on the addition of feed addictive The ratio of the 0.1% of weight, the feed addictive of the gained of embodiment 1~7 is added in basal feed, for feeding trial group 1~7 broiler chicken;The feed addictive of the gained of comparative example 1~4 is added in basal feed, for feeding experimental group 8~12 Broiler chicken;Commercially available new fowl is respectively adopted and founds to obtain poultry premix, commercially available wealth poultry nutrition treasured feeding trial group 13~14 logical greatly Broiler chicken;Control group broiler chicken is fed using basal feed.Broiler chicken free choice feeding, free water routinely carries out immune and expelling parasite, Daily feed intake record and broiler chicken death condition record, totally 40 days experimental period are carried out in experimental period.Weighed during off-test, calculated Average weight is drawn, and is calculated as follows index:
(1) average daily gain=(inventory-surplus doses)/test period;
(2) average daily gain quality=(average end quality-average prothyl amount)/test period;
(3) feedstuff-meat ratio=average daily gain/average daily gain quality;Feeding effect is shown in Tables 1 and 2.
Influence of the feed addictive of table 1 to meat chicken growth performance
As shown in Table 1, be with the addition of in the feed of test group 1~7 leaf containing dalbergia wood that the embodiment of the present invention 1~7 provided into Point fermented feed additive, broiler chicken within the feeding cycle of 40 days the average day increment of broiler weight 49g/ days with On, hence it is evident that the control group that the test group 12~13 and basal feed fed higher than commercially available prod are fed, show feed of the invention The stomach health of broiler chicken can be effectively protected, promotes gastrointestinal disturbances to absorb, improve the feed intake of broiler chicken, increase broiler weight.Separately Outward, feedstuff-meat ratio of the broiler chicken of test group 1~7 within the feeding cycle of 40 days is below 1.95, and test group 12~13 and control group More than 2.1, this illustrates fermented feed additive containing dalbergia wood leaf composition provided by the present invention to the feedstuff-meat ratio of middle broiler chicken Broiler chicken can be made preferably to digest and assimilate feed, therefore can effectively save feeding cost, increase cultivation income.
The broiler chicken of test group 8~the 10 average day increment of broiler weight and feedstuff-meat ratio within the feeding cycle of 40 days are significantly lower than Test group 1~7, this secondary metabolite produced mainly due to mould and root arrhizus fermentation Chinese herbal medicine helps to strengthen broiler chicken Digest and assimilate effect;And the derived essential oil of dalbergia wood leaf has the effect of inhibitory activity to bacillus subtilis and aspergillus niger, no Beneficial to bacillus subtilis and fermentation of Aspergillus niger Chinese herbal medicine secondary metabolite is produced, so that broiler chicken digests and assimilates effect Less than experimental group 1~7.
In the feed addictive of test group 11, herbal raw material includes dalbergia wood heartwood, feeding cycle of the broiler chicken at 40 days , significantly lower than the broiler chicken in test group 1~7, this is illustrated compared with dalbergia wood heartwood for the interior average day increment of broiler weight and feedstuff-meat ratio, The active ingredient of dalbergia wood leaf is more conducive to strengthen the growth digested and assimilated effect, be conducive to broiler chicken of broiler chicken.
Influence of the feed addictive of table 2 to broiler chicken survival condition
Group Feed addictive The incidence of disease (%) The death rate (%)
1 Feed addictive one 4.33 2.00
2 Feed addictive two 3.67 1.33
3 Feed addictive three 4.33 1.67
4 Feed addictive four 4.67 2.00
5 Feed addictive five 3.67 1.33
6 Feed addictive six 4.00 1.33
7 Feed addictive seven 5.33 1.67
8 Feed addictive eight 8.33 4.00
9 Feed addictive nine 8.67 6.67
10 Feed addictive ten 7.33 5.33
11 Feed addictive 11 4.67 1.67
12 Commercially available new fowl stands to obtain poultry premix 11.67 7.67
13 Commercially available wealth poultry nutrition logical greatly is precious 12.33 6.67
Control Without feed addictive 16.33 8.67
As shown in Table 2, be with the addition of in the feed of test group 1~7 leaf containing dalbergia wood that the embodiment of the present invention 1~7 provided into The fermented feed additive for dividing, broiler chicken broiler chicken incidence of disease and death rate within the feeding cycle of 40 days are below commercially available chicken feed The control group that the test group 12~13 and basal feed of nursing are fed.This explanation hair containing dalbergia wood leaf composition provided by the present invention Ferment type feed addictive can increase substantially the immunity of broiler chicken, reduce the morbidity and mortality of broiler chicken, be conducive to improving The benefit of plant.
Apparently higher than test group 1~7, this illustrates mould, root arrhizus fermentation to the morbidity and mortality of the broiler chicken of test group 8~10 The secondary metabolite (such as n- palmitic acids) that Chinese herbal medicine is produced, helps to strengthen the immunity of broiler chicken.
The morbidity and mortality of the broiler chicken of test group 11 are similar to test group 1~7, and this explanation is in other herbal component phases In the case of, the active ingredient in dalbergia wood leaf and heartwood is conducive to strengthening the immunity of broiler chicken.But raised after being added into heartwood The cost of material increases, cost performance reduction.
The above is only the preferred embodiment of the present invention, it should be pointed out that be ordinary skill for the art For personnel, under the premise without departing from the principles of the invention, some improvements and modifications can also be made, these improvements and modifications Should be regarded as protection scope of the present invention.

Claims (10)

1. a kind of fermented feed additive containing dalbergia wood leaf composition, it is characterised in that the fermented type containing dalbergia wood leaf composition The raw material of feed addictive, including herbal raw material, water and composite ferment, wherein, the weight of the composite ferment is institute State herbal raw material weight 0.02~0.1 times;The weight of the water is 0.4~1 times of the herbal raw material weight;
The herbal raw material, counts by weight, including:40~60 parts of dalbergia wood leaf, 35~50 parts of fly maggot larva, Herba Andrographitis 3 ~10 parts, 2~10 parts of Coleus forskohlii, 2~8 parts of broomcorn straw, 5~10 parts of balloonflower root, 3~12 parts of fructus cannabis, fructus alpiniae oxyphyllae 3~12 Part, 3~12 parts of hot pepper leaf, 5~10 parts in one's early teens, it is 1~6 part of the Fen, Amorphophalus rivieris of acer truncatum pollen 2~10,1~6 part of thick wood-fern rhizome, floating 5~10 parts of duckweed, 2~8 parts of Fructus Aurantii, 3~7 parts of loguat leaf, 1~5 part of beautiful mapler leaf;
The composite ferment includes one or more in mould, head mold, bacillus subtilis, aspergillus niger.
2. the fermented feed additive of dalbergia wood leaf composition is contained as claimed in claim 1, it is characterised in that the composite fermentation The weight of agent is 0.04~0.08 times of the herbal raw material weight.
3. the fermented feed additive of dalbergia wood leaf composition is contained as claimed in claim 1, it is characterised in that the weight of the water It is 0.4~0.8 times of the herbal raw material weight.
4. the fermented feed additive of dalbergia wood leaf composition is contained as claimed in claim 1, it is characterised in that the Chinese herbal medicine is former Material, counts by weight, including:50~60 parts of dalbergia wood leaf, 35~45 parts of fly maggot larva, 3~6 parts of Herba Andrographitis, Coleus forskohlii 2 ~8 parts, 2~5 parts of broomcorn straw, 5~8 parts of balloonflower root, 3~8 parts of fructus cannabis, 3~8 parts of fructus alpiniae oxyphyllae, 3~8 parts of hot pepper leaf, in one's early teens 5~10 parts, 2~5 parts of the Fen, Amorphophalus rivieris of acer truncatum pollen 2~7,2~5 parts of thick wood-fern rhizome, 5~10 parts of duckweed, 2~5 parts of Fructus Aurantii, Pi 3~7 parts of rake leaf, 2~4 parts of beautiful mapler leaf.
5. the fermented feed additive of dalbergia wood leaf composition is contained as claimed in claim 1, it is characterised in that the composite fermentation Agent includes mould and head mold, and the mould is 1 with the weight ratio of head mold:1~5.
6. a kind of preparation method of the fermented feed additive containing dalbergia wood leaf composition, it is characterised in that comprise the following steps:
(1) herbal raw material of following portions by weight is taken:40~60 parts of dalbergia wood leaf, 35~50 parts of fly maggot larva, Herba Andrographitis 3~10 Part, 2~10 parts of Coleus forskohlii, 2~8 parts of broomcorn straw, 5~10 parts of balloonflower root, 3~12 parts of fructus cannabis, 3~12 parts of fructus alpiniae oxyphyllae, 3~12 parts of hot pepper leaf, 5~10 parts in one's early teens, 1~6 part of the Fen, Amorphophalus rivieris of acer truncatum pollen 2~10,1~6 part of thick wood-fern rhizome, duckweed 5 ~10 parts, 2~8 parts of Fructus Aurantii, 3~7 parts of loguat leaf, 1~5 part of beautiful mapler leaf, co-grinding, be obtained Chinese herbal medicine powder;
(2) it is 0.4~1 according to the mass ratio of water and the herbal raw material:1 ratio, by water with it is described obtained by step (1) Defibrination after Chinese herbal medicine powder mixing, is obtained slurries;
(3) it is 0.02~0.1 according to the mass ratio of composite ferment and the herbal raw material:1 ratio, by the compound hair Ferment agent mixes with the slurries obtained by step (2), is fermented 3~8 days under conditions of 22~30 DEG C, and feed is obtained through low temperature drying adds Plus agent, wherein, the composite ferment includes one or more in mould, head mold, bacillus subtilis, aspergillus niger.
7. the preparation method of the fermented feed additive of dalbergia wood leaf composition is contained as claimed in claim 6, it is characterised in that institute State in step (1), the size of the Chinese herbal medicine powder is 20~50 mesh.
8. the preparation method of the fermented feed additive of dalbergia wood leaf composition is contained as claimed in claim 6, it is characterised in that institute State in step (3), the water content of the feed addictive is not higher than 15%.
9. a kind of fermented feed additive containing dalbergia wood leaf composition, it is characterised in that the fermented type containing dalbergia wood leaf composition Feed addictive is the preparation method system using the fermented feed additive containing dalbergia wood leaf composition as claimed in claim 6 .
10. application of the fermented feed additive containing dalbergia wood leaf composition as claimed in claim 1 in animal and fowl fodder is prepared.
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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107050184A (en) * 2017-02-27 2017-08-18 仲恺农业工程学院 A kind of peach blossom ma bartender's health-care oral liquid and its preparation method and application
CN107981097A (en) * 2017-11-25 2018-05-04 广州智龙生物科技有限公司 Promote the nutrient and its preparation and application of aquatic crustacean health
CN108576467A (en) * 2018-04-17 2018-09-28 林冬冬 It is a kind of using sweetgum leaf, chrysanthemum pears leaf, eaglewood leaf as chicken feed of raw material and preparation method thereof
CN112335779A (en) * 2020-10-21 2021-02-09 宜宾学院 Mixed silage of alpinia japonica and sweet sorghum and preparation method thereof

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20090095360A (en) * 2008-03-05 2009-09-09 대한민국(관리부서:농촌진흥청장) Feedstuff additives containing Xanthophyll and Preparing method thereof
CN105248980A (en) * 2015-11-20 2016-01-20 段现英 Broiler feed

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20090095360A (en) * 2008-03-05 2009-09-09 대한민국(관리부서:농촌진흥청장) Feedstuff additives containing Xanthophyll and Preparing method thereof
CN105248980A (en) * 2015-11-20 2016-01-20 段现英 Broiler feed

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
候红萍: "《发酵食品工艺学》", 31 December 2016, 中国农业大学出版社 *
武英: "《肉猪产业先进技术全书》", 31 December 2011, 山东科学技术出版社 *
陈默君: "《中国饲用植物》", 31 December 2002, 中国农业出版社 *

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107050184A (en) * 2017-02-27 2017-08-18 仲恺农业工程学院 A kind of peach blossom ma bartender's health-care oral liquid and its preparation method and application
CN107981097A (en) * 2017-11-25 2018-05-04 广州智龙生物科技有限公司 Promote the nutrient and its preparation and application of aquatic crustacean health
CN107981097B (en) * 2017-11-25 2021-07-09 广东渔赞臣生物科技有限公司 Nutrient for promoting health of crustacean of aquatic crustacean, and preparation and application methods thereof
CN108576467A (en) * 2018-04-17 2018-09-28 林冬冬 It is a kind of using sweetgum leaf, chrysanthemum pears leaf, eaglewood leaf as chicken feed of raw material and preparation method thereof
CN112335779A (en) * 2020-10-21 2021-02-09 宜宾学院 Mixed silage of alpinia japonica and sweet sorghum and preparation method thereof

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