CN103975850A - Cultivating method for hybrid rice - Google Patents
Cultivating method for hybrid rice Download PDFInfo
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- CN103975850A CN103975850A CN201410238499.XA CN201410238499A CN103975850A CN 103975850 A CN103975850 A CN 103975850A CN 201410238499 A CN201410238499 A CN 201410238499A CN 103975850 A CN103975850 A CN 103975850A
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Abstract
The invention discloses a cultivating method for hybrid rice. The culturing method comprises the following steps: (S1) with W3660 as a female parent, changing the entire shearing of stamen anther in rice glume shells into half shearing or less without damaging stigmas, then immediately spraying water into castration glume shells, wetting anther, flicking the dried anther extending out of the glume shells with fingers to remove the anther, and then sheathing with paper bags so as to wait for pollination; and (S2) with Nanjing 44 as a male parent, shearing off male parent ears before full flowers of male parent ears come, shearing off flowered ears, wetting the rest of ears with water, then drying, putting the dried ears into female ears sheathed with paper bags, sealing the paper bags, dithering the male parent ears when a great number of male parent ears flower, pollinating one by one, and hybridizing. Through the scheme, the culturing method is beneficial to free matching of male parents and female parents, simple in production flow, easy to control, and high in hybridization success rate, and the cultivated rice has the characteristics of being resistant to herbicides, low in glutelin and capable of resisting stripe disease.
Description
Technical field
The present invention relates to paddy rice cross breeding field, be specifically related to the breeding method of a kind of hybrid rice.
Background technology
China is Rice Cropping big country, in recent years along with the subsidy policy of country to Farming, and the growth of paddy purchasing price, Monitoring of Paddy Rice Plant Area has had stable increase.Within 2011, according to Ministry of Agriculture's investigation, national Monitoring of Paddy Rice Plant Area increased by 1.9% than upper one year, and wherein early rice cultivated area reaches 8,869 ten thousand mu, increases by 1,750,000 mu than last year.The per mu yield of paddy rice is also along with the popularization of new rice variety and the breeding corresponding cultivation techniques height of constantly bringing forth new ideas.
But China is populous nation, the four corners of the world, Rice Cropping region is vast in territory, and plantation climatic zone is various.And in recent years, the natural calamity of China takes place frequently, and it is occurred frequently that damage by disease and insect is, diversity state.Breeding high-yield, wide suitable, how anti-new rice variety are the important leverages of guarantee Food Security in China.
Summary of the invention
For addressing the above problem, the invention provides the breeding method of a kind of hybrid rice, method is simple, is convenient to implement, and is hybridized to power high.
For achieving the above object, the technical scheme that the present invention takes is:
A breeding method for hybrid rice, comprises the steps:
S1, taking W3660 as maternal, stamen flower pesticide in rice glume is cut off to change into completely and cut off half or still less, then in the clever shell after emasculation, spray water at once, flower pesticide is drenched, etc. shrivelled flower pesticide stretch out after clever shell with finger flick several under, shrivelled flower pesticide Du can be fallen by bullet, then puts paper bag, waits for pollination;
S2, on the south round-grained rice 44 be male parent, before male parent fringe Sheng flower is come, male parent fringe is cut, cut off and opened colored tassel, remaining fringe water is drenched, then dry on female fringe of putting into cover paper bag, paper bag mouth is sealed, shake male parent fringe when male parent fringe is bloomed in a large number soon, pollination, hybridizes one by one;
S3, from hybrid F_2, in filial generation colony, select the SDS-PAGE strip type of albumen of holoprotein in the main fringe of plant 12 rice consistent with W3660 holoprotein banding pattern, breeding time, plant height, the individual plant that strain leaf morphological characters is consistent with southern round-grained rice 44;
S4, herbicide resistance gene is imported in the individual plant of gained in S1 step;
S5, cultivation, seed selection, obtain new varieties.
Before described female parent and parent's sowing, need strict elite seed, and airing seed is after 1-2 days, by 500 times of immersion kinds of 40% formalin.First seed is soaked 1～2 day with clear water, suction moisture and do not show money or valuables one carries unintentionally as degree taking seed, then take out and dry, putting into formalin liquid soaks 48 hours, after clear water rinses, puts into ultrasonic sound appratus induction and germinates.
Wherein, the precocious late round-grained rice new varieties of high-quality that southern Jing44Shi You Cereal Crops Research Inst., Jiangsu Agricultural Science Academy is bred as, 2007 by the authorization of the kind committee of Jiangsu Province, is adapted at planting under the better-than-average soil fertility condition in Along The Yangtze River In Jiangsu Province and southern area of Jiangsu Province.This kind plant type compactness, growing way is more prosperous, and fringe type is medium, and tillering ability is stronger, and leaf look light green, colony's neat and consistent, the later stage, ripe look good, and lodging resistance is strong.About the 155 days time of infertility, than No. 7, round-grained rice " military fortune " Zao 3～5 days.Its main feature is stripe virus disease resistance, rice is of fine quality, lodging resistance is strong, yield stability good, wide adaptability.
Stripe virus disease resistance: the outstanding feature of southern round-grained rice 44 is to have stronger stripe virus disease resistance.Since within 2004, being bred as, successively in Nanjing, Zhangjiagang, Jiangyin, Wuxi, Danyang, Jingjiang, Jiangyan City, Nantong, salt all etc. carry out multiple spot anti-stripe virus disease qualification for many years in ground, all show anti-stripe virus disease, Institute of Plant Protection of academy of agricultural sciences of province field resistance qualification result in 2006: southern round-grained rice 44 cave incidence of disease 4.1%-8.1%, performance water resistant rice stripe, and resistance is stable.Meanwhile, to rice blast ZB
21, ZC
15and ZF
13 biological strains all show as immunity, bacterial blight-resisting.2007-2008 observes, and black streak dwarf also seldom occurs.
Rice quality is excellent, food mouthful property is good: analyze according to Ministry of Agriculture's rice and quality of item supervision and inspection center, south round-grained rice 44 rice quality physical and chemical indexs are brown rice rate 85.4%, polished rice rate 77.2%, head rice rate 75.7%, the white rate 15% of chalk, chalkiness degree 1.2%, 1 grade of transparency, amylose content 16.1%, glue denseness 75mm, the index of quality reaches GB secondary high quality paddy standard.All to hide good Wu-Yu-Geng 3 suitable with current Jiangsu Province food for its glossiness, fragrance, elasticity and overall target, and softness and viscosity are also better, have the advantages that food flavor is salubrious, mouthfeel is good.Therefore, this kind is by Gaoyou Shuan Tu meter industry company as fine quality rice development object, and the common paddy of cost ratio this season of average purchase is high by 10%.
Yielding ability, yield stability are good: this kind fringe type is larger, and tillering ability is stronger, and leaf look light green, and colony's regularity is good, and the later stage, ripe look good, and ripening rate is more than 90%.Thousand kernel weight 26-27 gram, community qualification result in 2003, amounts to 680 kilograms of per mu yields, and 641 kilograms of per mu yields are amounted in community multiple spot qualification in 2004, than " force is educated round-grained rice No. 7 " volume increase 3.7%.Within 2006, participate in Jiangsu Province's production test, 624.9 kilograms of average yield per mus, than No. 7 volume increase 7.1% of the military fortune of contrast round-grained rice.2004-2007 in Yangzhou, the ground demonstration plant such as Taizhou, Nantong, Nanjing, Zhenjiang, Changzhou, Wuxi, Suzhou, performance high and stable yields, average yield per mu is all 650 kilograms of left and right, the highest per unit area yield exceedes 780 kilograms.General every mu of 18-22 ten thousand fringes, every fringe 130-160 grain, the fringe kernel structure coordination ability is strong.
Lodging resistance is strong: this product plant is shorter, and stem stalk is sturdy, and basal internode is short, and lodging resistance is strong.Suffer respectively " Wei Pa ", " Luo Sha " twice violent typhoon on September 18th, 2007, October 7, most kind large area lodging, but southern round-grained rice 44 lodges hardly.
Wide adaptability: this kind is eared short to maturation time, and filling speed is fast.About the 155 days time of infertility, though belong to precocious late round-grained rice type, heading, than " No. 7, military fortune round-grained rice " Zao 2-3 days, ripe early 3～5 days, is therefore adapted at planting under the better-than-average soil fertility condition in slow ripe intermediate keng rice southern areas, Jiangsu and precocious Wan Jing district.Fringe kernel structure capacity of self-regulation is strong, easily plants easily pipe, high and stable yields, and utilization and extention risk is little.Meanwhile, due to ripe phase of this kind early, of short stem anti-fall, be applicable to the multiple light simple planting types such as the machine transplanting of rice, machine are live, rice transplanting.
Described herbicide resistance gene is anti-herbicide gene bxn or the bar structure of connecting with male sterile gene TA29-barnase.
Such scheme, is conducive to freely matching of Parent, and production procedure is simple, is easy to control, and is hybridized to power high, and the paddy rice of cultivating has the characteristic of antiweed, low glutelin and anti-stripe virus disease.
Embodiment
In order to make objects and advantages of the present invention clearer, below in conjunction with embodiment, the present invention is further elaborated.Should be appreciated that specific embodiment described herein, only in order to explain the present invention, is not intended to limit the present invention.
Embodiment mono-
A breeding method for hybrid rice, comprises the steps:
S1, taking W3660 as maternal, stamen flower pesticide in rice glume is cut off to change into completely and cut off half or still less, then in the clever shell after hero, water at once, flower pesticide is drenched, etc. shrivelled flower pesticide stretch out after clever shell with finger flick several under, shrivelled flower pesticide Du can be fallen by bullet, then puts paper bag, waits for pollination;
S2, on the south round-grained rice 44 be male parent, male parent fringe contain flower come before (minority grain husk shell start to bloom), male parent fringe is cut, cut off and opened colored tassel, remaining fringe water is drenched, then dry on female fringe of putting into cover paper bag, paper bag mouth is sealed.Shake male parent fringe when male parent fringe is bloomed in a large number soon, the pollen on male parent fringe will not have any loss.In advance male parent fringe is all sheared, put into wooden fringe, wait while blooming, pollination one by one, has greatly improved operating efficiency and has been hybridized to power.This ratio juris is: the time of a tassel blossom and temperature in one day, humidity, kind three factors are relevant.Paddy rice General Requirements temperature is more than 20 DEG C, and relative moisture could normally be bloomed higher than 60%, the optimum temperature of blooming 28-32 DEG C, and the suitableeest relative moisture is 80-90%.The optimum temperature of blooming is come interim, and paddy rice will enter full-bloom stage, if now humidity is less than 60% not, unfavorable its be bloomed.In new method, male parent fringe is drenched and put into paper bag after drying and seal, come temporarily when the optimum temperature of blooming, the humidity in the paper bag of sealing has reached 80-90%, the blooming of favourable male parent fringe;
S3, from hybrid F_2, in filial generation colony, select the SDS-PAGE strip type of albumen of holoprotein in the main fringe of plant 12 rice consistent with W3660 holoprotein banding pattern, breeding time, plant height, the individual plant that strain leaf morphological characters is consistent with southern round-grained rice 44;
S4, herbicide resistance gene is imported in the individual plant of gained in S1 step;
S5, cultivation, seed selection, obtain new varieties.
Before described female parent and parent's sowing, need strict elite seed, and airing seed is after 1-2 days, by 500 times of immersion kinds of 40% formalin.First seed is soaked 1～2 day with clear water, suction moisture and do not show money or valuables one carries unintentionally as degree taking seed, then take out and dry, putting into formalin liquid soaks 48 hours, after clear water rinses, puts into ultrasonic sound appratus induction and germinates.
Described herbicide resistance gene is anti-herbicide gene bxn or the bar structure of connecting with male sterile gene TA29-barnase.
This specifically implements and is beneficial to freely matching of Parent, and production procedure is simple, is easy to control, and is hybridized to power high, and the paddy rice of cultivating has the characteristic of antiweed, low glutelin and anti-stripe virus disease;
The above is only the preferred embodiment of the present invention; it should be pointed out that for those skilled in the art, under the premise without departing from the principles of the invention; can also make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.
Claims (3)
1. a breeding method for hybrid rice, is characterized in that, comprises the steps:
S1, taking W3660 as maternal, stamen flower pesticide in rice glume is cut off to change into completely and cut off half or still less, do not damage column cap, then in the clever shell after emasculation, spray water at once, flower pesticide is drenched, wait shrivelled flower pesticide stretch out after clever shell with finger flick several under, flick off flower pesticide, then put paper bag, wait for pollination;
S2, on the south round-grained rice 44 be male parent, before male parent fringe Sheng flower is come, male parent fringe is cut, cut off and opened colored tassel, remaining fringe water is drenched, then dry on female fringe of putting into cover paper bag, paper bag mouth is sealed, shake male parent fringe when male parent fringe is bloomed in a large number soon, pollination, hybridizes one by one;
S3, from hybrid F_2, in filial generation colony, select the SDS-PAGE strip type of albumen of holoprotein in the main fringe of plant 12 rice consistent with W3660 holoprotein banding pattern, breeding time, plant height, the individual plant that strain leaf morphological characters is consistent with southern round-grained rice 44;
S4, herbicide resistance gene is imported in the individual plant of gained in S1 step;
S5, cultivation, seed selection, obtain new varieties.
2. the breeding method of a kind of hybrid rice according to claim 1, it is characterized in that, before described female parent and parent's sowing, need strict elite seed, and airing seed is after 1-2 days, by 500 times of immersion kinds of 40% formalin, first seed is soaked 1～2 day with clear water, suction moisture and do not show money or valuables one carries unintentionally as degree taking seed, then taking out and dry, putting into formalin liquid soaks 48 hours, after clear water rinses, put into ultrasonic sound appratus induction and germinate.
3. the breeding method of a kind of hybrid rice according to claim 1, is characterized in that, described herbicide resistance gene is anti-herbicide gene bxn or the bar structure of connecting with male sterile gene TA29-barnase.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104221843A (en) * | 2014-09-12 | 2014-12-24 | 中国热带农业科学院热带生物技术研究所 | Rice blooming promotion device and method for manual castration by promoting rice blooming through xylene |
| CN105660363A (en) * | 2016-01-20 | 2016-06-15 | 安徽理想种业有限公司 | Rice hybridizing and detasseling method |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101347094A (en) * | 2008-08-22 | 2009-01-21 | 南京农业大学 | Method for breeding rice vairety with polymerized low-glutelin and rice stripe-resistance |
| CN103053409A (en) * | 2013-01-17 | 2013-04-24 | 西南科技大学 | Method for improving test-crossing and back-crossing efficiency during breeding process of hybrid rice |
| CN103733984A (en) * | 2014-01-22 | 2014-04-23 | 泗洪县原种场丰收种业有限公司 | Castration method for rice hyberdization |
-
2014
- 2014-06-03 CN CN201410238499.XA patent/CN103975850A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101347094A (en) * | 2008-08-22 | 2009-01-21 | 南京农业大学 | Method for breeding rice vairety with polymerized low-glutelin and rice stripe-resistance |
| CN103053409A (en) * | 2013-01-17 | 2013-04-24 | 西南科技大学 | Method for improving test-crossing and back-crossing efficiency during breeding process of hybrid rice |
| CN103733984A (en) * | 2014-01-22 | 2014-04-23 | 泗洪县原种场丰收种业有限公司 | Castration method for rice hyberdization |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104221843A (en) * | 2014-09-12 | 2014-12-24 | 中国热带农业科学院热带生物技术研究所 | Rice blooming promotion device and method for manual castration by promoting rice blooming through xylene |
| CN105660363A (en) * | 2016-01-20 | 2016-06-15 | 安徽理想种业有限公司 | Rice hybridizing and detasseling method |
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Application publication date: 20140813 |
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