CN103947437B - Chinese yew non-line-of-sight error method - Google Patents
Chinese yew non-line-of-sight error method Download PDFInfo
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Abstract
The invention discloses a kind of Chinese yew non-line-of-sight error method, comprise the following steps: (1) branch is selected: select the new branch that Taxus x media is raw then, cut and obtain thick, thin two bundle branches, stand-by; (2) medium of seedling bed process: deep ploughing, opens railway carriage or compartment and establishes canopy, and sterilization, sprinkles turfy soil, spreads thin river sand; (3) taking root liquid preparation: comprise amino acid, nitrogen, phosphorus, potassium, calcium, and methyl α-naphthyl acetate, draw diindyl butyric acid, alcohol, white sugar, ABT root-growing agent; (4) branch is soaked: soak in the taking root liquid that withe step (1) obtained obtains in step (3); (5) skewer is inserted; (6) seedbed insulation, moisturizing.The present invention by having carried out technological innovation in branch selection, classification, medium of seedling bed process, taking root liquid configuration, classification immersion, achieve the object that survival rate is high, reproduction speed is fast, skewer breeding Chinese yew can be inserted fast on a large scale, be conducive to amount reproduction and the anti-cancer applications of Chinese yew.
Description
Technical field
The present invention relates to a kind of Chinese yew and insert skewer propagation method, particularly relate to a kind of Chinese yew non-line-of-sight error method that survival rate is high, reproduction speed is fast.
Background technology
Taxus is in shallow rooted plant, its main root is not obvious, side root flourishing, generally acknowledge endangered natural rare plants for anticancer in the world, collect medicinal, view and admire, material for one, be referred to as visually precious " eucalyptus " and " cash cow " by people, with Taxus x media be that " taxol " that primary raw material extracts is a kind of structure uniqueness, performance safety, effectively can kill the natural plant product of the broad spectrum anticancer of cancer cell, there is fabulous antitumaous effect.
Owing to having shortcomings such as sprouting at the bottom of comparatively slow (needing 2 years) cycle length, percentage of seedgermination, seedling growth is slow with Chinese yew seminal propagation nursery stock, so adopt slotting skewer propagation method breeding Chinese yew to be more satisfactory selection.Application cuttage technique quickly breeding Chinese yew nursery stock, have seedling bend strengthen, take root fast, side root is flourishing, survival rate is high, grow feature rapidly, be solve Chinese yew artificial afforestration, accelerate the most effective approach that Chinese yew Medical forests is built.
1996, China national the Ministry of Forestry has introduced graceful ground subrace from Canada in the first batch and has carried out plantation cultivation at Beichuan, Sichuan Tang Jiaping and forest farm, Hongya, afterwards again in Xi'an, Shaanxi normal celestial movement biotech firm, Hanzhong Heng Tai biotech firm, the plantation of Chengdu Di Zhen biotech firm, the cuttage technique that these units generally adopt comprised:
1, cut as cuttage maternal with 2 to 3 years branches, be cut into 10 to 15 centimeters long, do not pull out pin leaf, side shoot, spray are not used in cuttage female parent;
2, seldom sterilize or simply sterilize with conventional disinfection method in seedbed;
3, root-growing agent is more single, just inserts, the overlong time of some bubbles with common root-growing agent bubble is lower, common root-growing agent does not add amino acid and other trace elements, so cuttage survival rate is comparatively complied with, it is slow to go out transplantation of seedlings, general one ton of branch survives 8 to 10 ten thousand strains, and within 18 to 20 months, ability is transplanted.
Traditional Chinese yew inserts skewer propagation method just because of there is above-mentioned defect, so be difficult to be formed extensive effect of inserting skewer breeding Chinese yew fast, constrains amount reproduction and the anti-cancer applications of Chinese yew.
Summary of the invention
Object of the present invention provides the Chinese yew that a kind of survival rate is high, reproduction speed is fast to insert skewer propagation method with regard to being in order to solve the problem.
The present invention is achieved through the following technical solutions above-mentioned purpose:
A kind of Chinese yew inserts skewer propagation method, comprises the following steps:
(1) branch is selected: select the new branch that Taxus x media is raw then, first cut side shoot, sprout, then cuts major branch, thick branch, the branch cut is pulled out the pin leaf of about 2 cm, opens binding, obtain two bundle branches according to thick, segmentation, stand-by;
(2) medium of seedling bed process: medium of seedling bed is deep ploughed, and then opens railway carriage or compartment and establishes canopy, at greenhouse bracket upper cover plastic film and sunshade net, more loose medium of seedling bed, the thin river sand of about 1 cm thick that carries out nursery bed disinfection with insecticide, bactericide, sprinkle turfy soil, spreads;
(3) taking root liquid preparation: with amino acid, nitrogen, phosphorus, potassium, calcium, and methyl α-naphthyl acetate, draw diindyl butyric acid, alcohol, white sugar, ABT root-growing agent, stir and obtain taking root liquid, concrete process for preparation is: dissolved by ABT root-growing agent alcohol, then aquae destillata is added or cold water stirs, add other raw material to stir and obtain stoste of taking root, be diluted to desired concn during use and obtain taking root liquid, above-mentioned nitrogen, phosphorus, potassium, calcium can add by various compound form;
(4) branch is soaked: soak 2 ~ 5 minutes in the taking root liquid that withe step (1) obtained obtains in step (3), thick branch soaks about 100 minutes;
(5) skewer is inserted: the branch after soaking is inserted skewer on the seedbed processed through step (2), and the withe cuttage degree of depth is about 2 centimetres, is limited to touch turfy soil, and the thick stem cutting skewer degree of depth is about 2 centimetres, spreads the precious strain rock of about 1 cm thick after being plugged on medium of seedling bed;
(6) seedbed insulation, moisturizing: spray water with automatic micro-spray system, keep canopy temperature to be no more than 26C °, humidity remains between 75-80%, supplements the nutrients, regularly sterilize with bactericide by weekly frequency potassium dihydrogen phosphate.
Above-mentioned Taxus x media (Taxus X media), originating in the U.S., Canada, is a kind of natural hybrid variety, and its female parent is taxus chinensis in northeast (T.cuspidata), male parent is european yew (T.baccata), in the U.S., Canada's growth development history of existing nearly 100 years.
Above-mentioned abt root-inducing powder is a kind of wide spectrum, efficient, compound plant growth regulator, and it is succeeded in developing by Chinese forest-science academy Wang Tao academician, extensively promotes throughout the country at present.
Particularly, in described step (1), two bundle branches are respectively withe 100 strain a bundle, thick branch 30 strain a bundle.
In described step (2), adopt rice field as base, seedbed, base, seedbed deep ploughs about 70 centimeters, railway carriage or compartment is opened by 1.3 meters wide in seedbed, and 4 seedbeds are a booth, and greenhouse bracket covers the plastic film of 10 meters wide, cover sunshade net again, then with artificial loose medium of seedling bed.
In described step (3), taking root liquid comprises the component of following mass parts: 80 ~ 120 parts, amino acid, 0.8 ~ 1.5 part, nitrogen, 1 ~ 3 part, phosphorus, 1 ~ 3 part, potassium, calcium 1 ~ 3 part, methyl α-naphthyl acetate 0.8 ~ 1.5 part, draw diindyl butyric acid 0.8 ~ 1.5 part, white sugar 30 ~ 60 parts, ABT root-growing agent 0.8 ~ 1.5 part, alcohol dissolves ABT root-growing agent be completely limited to meet.
Preferably, the mass parts of each component is: amino acid/11 10 parts, 1 part, nitrogen, 2 parts, phosphorus, 2 parts, potassium, calcium 2 parts, methyl α-naphthyl acetate 1 part, draw diindyl butyric acid 1 part, white sugar 50 parts, ABT root-growing agent 1 part, alcohol dissolves ABT root-growing agent be completely limited to meet.
Beneficial effect of the present invention is:
The present invention by having carried out technological innovation in branch selection, classification, medium of seedling bed process, taking root liquid configuration, classification immersion, achieve the object that survival rate is high, reproduction speed is fast, skewer breeding Chinese yew can be inserted fast on a large scale, be conducive to amount reproduction and the anti-cancer applications of Chinese yew.Be embodied in:
1, improve kind and insert skewer shoot survival percent, branch per ton is cultivated seedling and is reached 60-70 ten thousand strains, and cuttage survival rate reaches more than 90%, is that conventional method inserts 5-7 that skewer emerges doubly;
2, shorten growing-seedling period, cultivate 8 months to the 1 year seedbed output time of shortening than conventional method, 12 cross the moon can transplant, and keeps original gene constant, and plant from seedling to base, improve transplanting survival rate, transplanting survival rate reaches more than 95%.
3, the method is also applicable to the cuttage and quick-propagation nursery of plant with difficult rooting and rare species, allows rare species be bred very soon, increases economic benefit to plantation family.
Embodiment
Below in conjunction with specific embodiment, the invention will be further described:
Embodiment 1:
Adopt following steps:
(1) branch is selected: select the new branch that Taxus x media is raw then, first cut side shoot, sprout, cut major branch, thick branch again, the branch cut is pulled out the pin leaf of about 2 cm, binding is opened according to thick, segmentation, obtain two bundle branches, two bundle branches are respectively withe 100 strain a bundle, thick branch 30 strain a bundle, stand-by;
(2) medium of seedling bed process: adopt rice field as base, seedbed, base, seedbed deep ploughs about 70 centimeters, with manually sending thin earth, railway carriage or compartment is opened by 1.3 meters wide in seedbed, 4 seedbeds are a booth, greenhouse bracket covers the plastic film of 10 meters wide, cover sunshade net again, again with artificial loose medium of seedling bed, nursery bed disinfection is carried out with insecticide, bactericide, first holder carbendazim, tpn and formalin, sprinkle turfy soil, spread the thin river sand of about 1 cm thick, print on river sand with the flitch of customization;
(3) taking root liquid preparation: each component by following mass parts: amino acid/11 10 parts, 1 part, nitrogen, 2 parts, phosphorus, 2 parts, potassium, calcium 2 parts, methyl α-naphthyl acetate 1 part, draw diindyl butyric acid 1 part, white sugar 50 parts, ABT root-growing agent 1 part, alcohol dissolves ABT root-growing agent be completely limited to meet, stir and obtain taking root liquid, concrete process for preparation is: dissolved by ABT root-growing agent alcohol, then aquae destillata is added or cold water stirs, add other raw material to stir and obtain stoste of taking root, be diluted to desired concn during use and obtain taking root liquid;
(4) branch is soaked: soak 2 ~ 5 minutes in the taking root liquid that withe step (1) obtained obtains in step (3), thick branch soaks about 100 minutes;
(5) skewer is inserted: the branch after soaking is inserted skewer on the seedbed processed through step (2), for keeping the specification in seedbed, cuttage is carried out by the vestige of flitch printing when inserting skewer, keep seeding row spacing symmetrical, the withe cuttage degree of depth is about 2 centimetres, is limited to touch turfy soil, and the thick stem cutting skewer degree of depth is about 2 centimetres, on medium of seedling bed, spread the precious strain rock of about 1 cm thick after being plugged, play the effect of water conservation, insulation, anti-grass;
(6) seedbed insulation, moisturizing: spray water with automatic micro-spray system, canopy temperature is kept to be no more than 26C °, humidity remains between 75-80%, check the temperature and humidity in canopy at any time, when extraneous room temperature is higher or the lunar calendar is opened film shade net shelter from heat or light mid-March, weeds on timing removing seedbed, supplement the nutrients by weekly frequency potassium dihydrogen phosphate, regularly sterilize with bactericide.
By above-mentioned steps breeding Taxus x media seedling, branch per ton is cultivated seedling and is reached 60-70 ten thousand strains, and cuttage survival rate reaches more than 90%, is that conventional method inserts 5-7 that skewer emerges doubly; 12 cross the moon can transplant, and keeps original gene constant, and plant from seedling to base, improve transplanting survival rate, cuttage survival rate reaches more than 95%.
Embodiment 2:
On the basis of embodiment 1, in the taking root liquid configuration of step (3), each component by following mass parts: 80 parts, amino acid, 0.8 part, nitrogen, 1 part, phosphorus, 1 part, potassium, calcium 1 part, methyl α-naphthyl acetate 0.8 part, draw diindyl butyric acid 0.8 part, white sugar 30 parts, ABT root-growing agent 0.8 part, alcohol dissolves ABT root-growing agent be completely limited to meet, other each step is identical with embodiment 1, and effect is basically identical.
Embodiment 3:
On the basis of embodiment 1, in the taking root liquid configuration of step (3), each component by following mass parts: amino acid/11 20 parts, 1.5 parts, nitrogen, 3 parts, phosphorus, 3 parts, potassium, calcium 3 parts, methyl α-naphthyl acetate 1.5 parts, draw diindyl butyric acid 1.5 parts, white sugar 60 parts, ABT root-growing agent 1.5 parts, alcohol dissolves ABT root-growing agent be completely limited to meet, other each step is identical with embodiment 1, and effect is basically identical.
Above-described embodiment is preferred embodiment of the present invention; it is not the restriction to technical solution of the present invention; as long as without the technical scheme that creative work can realize on the basis of above-described embodiment, all should be considered as falling within the scope of the rights protection of patent of the present invention.
Claims (4)
1. a Chinese yew non-line-of-sight error method, is characterized in that: comprise the following steps:
(1) branch is selected: select the new branch that Taxus x media is raw then, first cut side shoot, sprout, then cuts major branch, thick branch, the branch cut is pulled out the pin leaf of 2 cm, opens binding, obtain two bundle branches according to thick, segmentation, stand-by;
(2) medium of seedling bed process: medium of seedling bed is deep ploughed, and then opens railway carriage or compartment and establishes canopy, at greenhouse bracket upper cover plastic film and sunshade net, more loose medium of seedling bed, the thin river sand of about 1 cm thick that carries out nursery bed disinfection with insecticide, bactericide, sprinkle turfy soil, spreads;
(3) taking root liquid preparation: with amino acid, nitrogen, phosphorus, potassium, calcium, and methyl α-naphthyl acetate, draw diindyl butyric acid, alcohol, white sugar, ABT root-growing agent, stir and obtain taking root liquid, concrete process for preparation is: dissolved by ABT root-growing agent alcohol, then aquae destillata is added or cold water stirs, add other raw material to stir and obtain stoste of taking root, be diluted to desired concn during use and obtain taking root liquid;
(4) branch is soaked: soak 2 ~ 5 minutes in the taking root liquid that withe step (1) obtained obtains in step (3), thick branch soaks about 100 minutes;
(5) skewer is inserted: the branch after soaking is inserted skewer on the seedbed processed through step (2), and the withe cuttage degree of depth is about 2 centimetres, is limited to touch turfy soil, and the thick stem cutting skewer degree of depth is about 2 centimetres, spreads the precious strain rock of about 1 cm thick after being plugged on medium of seedling bed;
(6) seedbed insulation, moisturizing: spray water with automatic micro-spray system, keep canopy temperature to be no more than 26C °, humidity remains between 75-80%, supplements the nutrients, regularly sterilize with bactericide by weekly frequency potassium dihydrogen phosphate;
In described step (3), taking root liquid comprises the component of following mass parts: 80 ~ 120 parts, amino acid, 0.8 ~ 1.5 part, nitrogen, 1 ~ 3 part, phosphorus, 1 ~ 3 part, potassium, calcium 1 ~ 3 part, methyl α-naphthyl acetate 0.8 ~ 1.5 part, draw diindyl butyric acid 0.8 ~ 1.5 part, white sugar 30 ~ 60 parts, ABT root-growing agent 0.8 ~ 1.5 part, alcohol dissolves ABT root-growing agent be completely limited to meet.
2. Chinese yew non-line-of-sight error method according to claim 1, is characterized in that: in described step (1), and two bundle branches are respectively withe 100 strain a bundle, thick branch 30 strain a bundle.
3. Chinese yew non-line-of-sight error method according to claim 1, it is characterized in that: in described step (2), adopt rice field as base, seedbed, base, seedbed deep ploughs about 70 centimeters, railway carriage or compartment is opened by 1.3 meters wide in seedbed, and 4 seedbeds are a booth, and greenhouse bracket covers the plastic film of 10 meters wide, cover sunshade net again, then with artificial loose medium of seedling bed.
4. Chinese yew non-line-of-sight error method according to claim 1, it is characterized in that: in described step (3), the mass parts of each component is: amino acid/11 10 parts, 1 part, nitrogen, 2 parts, phosphorus, 2 parts, potassium, calcium 2 parts, methyl α-naphthyl acetate 1 part, draw diindyl butyric acid 1 part, white sugar 50 parts, ABT root-growing agent 1 part, alcohol dissolves ABT root-growing agent be completely limited to meet.
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| CN104429780A (en) * | 2014-12-10 | 2015-03-25 | 黄平县源顺生态园有限公司 | Method for improving cutting survival rate of Taxus chinensis var mairei |
| CN105123754A (en) * | 2015-08-13 | 2015-12-09 | 宁夏大学 | Nursery stock vitality protection agent |
| CN106171762A (en) * | 2016-07-19 | 2016-12-07 | 泸州千秋辉煌农业有限公司 | A kind of method of wild Ramulus et folium taxi cuspidatae branch cutting breeding |
| CN106386372A (en) * | 2016-08-23 | 2017-02-15 | 黄万阳 | Rapid propagation method for grape nursery stock |
| CN106518350A (en) * | 2016-10-27 | 2017-03-22 | 六安市振清生态农业有限公司 | Nutritive organic matrix for taxus chinensis planting |
| CN107047262A (en) * | 2017-06-05 | 2017-08-18 | 四川格睿园林科技有限公司 | A kind of non-test tube seedling method of quick propagating and cultivating of Cotinus coggygria |
| CN107371987A (en) * | 2017-09-12 | 2017-11-24 | 贵州阳溪洪林中药材产业开发有限公司 | A kind of Yew cuttage breeding and seedling method |
| CN108575517A (en) * | 2018-05-25 | 2018-09-28 | 六枝特区舰航种养殖场 | A kind of implantation methods for improving Rosa roxburghii Tratt mouthfeel and improving Rosa roxburghii Tratt yield |
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| CN100588324C (en) * | 2008-02-29 | 2010-02-10 | 师进 | High active biological nutrition root-inducing powder |
| CN102598975A (en) * | 2012-02-16 | 2012-07-25 | 莲花县森美农林开发有限公司 | Method for breeding Taxus media |
| CN103444382A (en) * | 2012-05-28 | 2013-12-18 | 浙江百年红豆杉生物科技有限公司 | Cultivation technology for Taxus media |
| CN103621273A (en) * | 2012-08-23 | 2014-03-12 | 黄山市黟县环宇农业科技有限责任公司 | Method for manually cutting taxus chinensis for seedling cultivation |
| CN103283460B (en) * | 2013-06-07 | 2014-12-10 | 江苏红豆杉生物科技股份有限公司 | Taxus media cuttage method |
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