CN103946397B - 用于通用实时多重分析检测的双功能寡核苷酸探针 - Google Patents

用于通用实时多重分析检测的双功能寡核苷酸探针 Download PDF

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CN103946397B
CN103946397B CN201280055550.2A CN201280055550A CN103946397B CN 103946397 B CN103946397 B CN 103946397B CN 201280055550 A CN201280055550 A CN 201280055550A CN 103946397 B CN103946397 B CN 103946397B
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molecule
region
detection
mediator
probe
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CN103946397A (zh
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京特·罗斯
贝恩德·法尔丁
费利克斯·冯施特滕
西蒙·瓦德勒
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Albert Ludwigs Universitaet Freiburg
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6823—Release of bound markers
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    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes

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CN201280055550.2A 2011-11-10 2012-11-12 用于通用实时多重分析检测的双功能寡核苷酸探针 Active CN103946397B (zh)

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DE102011055247.2 2011-11-10
DE102011055247A DE102011055247A1 (de) 2011-11-10 2011-11-10 Multianalyt-Reportersystem
PCT/EP2012/072402 WO2013079307A1 (de) 2011-11-10 2012-11-12 Bifunktionale oligonukleotidsonde zur universellen echtzeit multianalytdetektion

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CN103946397B true CN103946397B (zh) 2021-03-16

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US (1) US11293053B2 (cg-RX-API-DMAC7.html)
EP (1) EP2776585B8 (cg-RX-API-DMAC7.html)
JP (1) JP6265905B2 (cg-RX-API-DMAC7.html)
CN (1) CN103946397B (cg-RX-API-DMAC7.html)
CA (1) CA2854427A1 (cg-RX-API-DMAC7.html)
DE (1) DE102011055247A1 (cg-RX-API-DMAC7.html)
ES (1) ES2698903T3 (cg-RX-API-DMAC7.html)
IN (1) IN2014DN04645A (cg-RX-API-DMAC7.html)
WO (1) WO2013079307A1 (cg-RX-API-DMAC7.html)

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US20140272959A1 (en) * 2013-03-14 2014-09-18 President And Fellows Of Harvard College Methods of Hybridizing Probes to Genomic DNA
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EP3034619B1 (de) * 2014-12-16 2018-10-17 microBIOMix GmbH Modifizierte Oligonukleotide und Verfahren zum Einschleusen von Primern oder Kontrollmolekülen oder Sonden in geschlossene PCR-Systeme
KR20170116157A (ko) * 2015-02-18 2017-10-18 싱귤러 바이오, 인코포레이티드 단일분자 검출용 분석 및 그의 용도
KR101875662B1 (ko) 2015-12-01 2018-08-02 에스디 바이오센서 주식회사 등온 핵산 증폭을 위한 리포터 염료, 퀀처가 포함되는 등온 기반 이중 기능성 올리고뉴클레오타이드 및 이를 이용한 핵산 증폭과 측정 방법
CN107024583B (zh) * 2016-01-29 2019-03-08 中国科学院苏州纳米技术与纳米仿生研究所 硫黄素t适配体、其筛选方法及应用
BR112019012925A2 (pt) 2016-12-23 2019-12-10 Albert-Ludwigs-Universität Freiburg sonda mediadora em duas partes
CN108823287B (zh) 2017-04-28 2019-04-12 厦门大学 一种检测靶核酸序列的方法
GB201713251D0 (en) * 2017-08-18 2017-10-04 Lgc Genomics Ltd Methods and kits for detection of nucleic acid molecules
CN111100935B (zh) * 2018-10-26 2023-03-31 厦门大学 一种细菌耐药基因检测的方法
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JP2025513429A (ja) 2022-04-22 2025-04-24 ハーン－シカード－ゲゼルシャフト フォー アングワント フォースクング イー．ブイ． 区別可能なシグナルの強さを有する標的配列非依存性レポーター分子によるｐｃｒ多重化
WO2023203230A1 (de) 2022-04-22 2023-10-26 Hahn-Schickard-Gesellschaft Für Angewandte Forschung E. V. Nukleinsäurenachweis in einer pcr mittels eines zielsequenz-unspezifischem modularer reporterkomplexes
EP4265734A1 (de) 2022-04-22 2023-10-25 Hahn-Schickard-Gesellschaft für angewandte Forschung e.V. Nukleinsäurenachweis in einer pcr mittels eines zielsequenz-unspezifischem modularer reporterkomplexes
EP4265735A1 (de) 2022-04-22 2023-10-25 Hahn-Schickard-Gesellschaft für angewandte Forschung e.V. Pcr-multiplexing durch zielsequenz-unabhängige-reportermoleküle mit unterscheid-baren signalstäken
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Publication number Publication date
EP2776585A1 (de) 2014-09-17
ES2698903T3 (es) 2019-02-06
EP2776585B1 (de) 2018-08-22
WO2013079307A1 (de) 2013-06-06
DE102011055247A1 (de) 2013-05-16
US11293053B2 (en) 2022-04-05
IN2014DN04645A (cg-RX-API-DMAC7.html) 2015-05-08
CA2854427A1 (en) 2013-06-06
EP2776585B8 (de) 2019-04-10
CN103946397A (zh) 2014-07-23
US20140315747A1 (en) 2014-10-23
JP2014533107A (ja) 2014-12-11
JP6265905B2 (ja) 2018-01-24

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