CN103946378B - 葡糖淀粉酶变体和编码它们的多核苷酸 - Google Patents
葡糖淀粉酶变体和编码它们的多核苷酸 Download PDFInfo
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- CN103946378B CN103946378B CN201280050093.8A CN201280050093A CN103946378B CN 103946378 B CN103946378 B CN 103946378B CN 201280050093 A CN201280050093 A CN 201280050093A CN 103946378 B CN103946378 B CN 103946378B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
- C12N9/2411—Amylases
- C12N9/2428—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
- C12N9/2411—Amylases
- C12N9/2414—Alpha-amylase (3.2.1.1.)
- C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
- C12N9/242—Fungal source
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/04—Alpha- or beta- amino acids
- C12P13/20—Aspartic acid; Asparagine
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/20—Preparation of compounds containing saccharide radicals produced by the action of an exo-1,4 alpha-glucosidase, e.g. dextrose
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P37/00—Preparation of compounds having a 4-thia-1-azabicyclo [3.2.0] heptane ring system, e.g. penicillin
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
- C12P5/002—Preparation of hydrocarbons or halogenated hydrocarbons cyclic
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
- C12P5/02—Preparation of hydrocarbons or halogenated hydrocarbons acyclic
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/06—Ethanol, i.e. non-beverage
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
- C12P7/44—Polycarboxylic acids
- C12P7/46—Dicarboxylic acids having four or less carbon atoms, e.g. fumaric acid, maleic acid
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
- C12P7/44—Polycarboxylic acids
- C12P7/48—Tricarboxylic acids, e.g. citric acid
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
- C12P7/54—Acetic acid
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
- C12P7/56—Lactic acid
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01001—Alpha-amylase (3.2.1.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01003—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Mycology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201161545628P | 2011-10-11 | 2011-10-11 | |
| US61/545,628 | 2011-10-11 | ||
| PCT/EP2012/070127 WO2013053801A1 (en) | 2011-10-11 | 2012-10-11 | Glucoamylase variants and polynucleotides encoding same |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103946378A CN103946378A (zh) | 2014-07-23 |
| CN103946378B true CN103946378B (zh) | 2018-04-24 |
Family
ID=47049149
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201280050093.8A Active CN103946378B (zh) | 2011-10-11 | 2012-10-11 | 葡糖淀粉酶变体和编码它们的多核苷酸 |
Country Status (10)
| Country | Link |
|---|---|
| US (8) | US9353362B2 (https=) |
| EP (1) | EP2766476B1 (https=) |
| CN (1) | CN103946378B (https=) |
| CA (1) | CA2853000C (https=) |
| DK (1) | DK2766476T3 (https=) |
| EA (1) | EA201490649A1 (https=) |
| ES (1) | ES2638669T3 (https=) |
| IN (1) | IN2014CN03468A (https=) |
| MX (1) | MX351762B (https=) |
| WO (1) | WO2013053801A1 (https=) |
Families Citing this family (54)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2061882A2 (en) * | 2006-10-10 | 2009-05-27 | Danisco US, INC., Genencor Division | Glucoamylase variants with altered properties |
| CA2903934A1 (en) * | 2013-03-07 | 2014-09-12 | Novozymes A/S | Glucoamylase variants and polynucleotides encoding same |
| US10035973B2 (en) | 2013-06-24 | 2018-07-31 | Novozymes A/S | Process of extracting oil from thin stillage |
| EP3022300B1 (en) | 2013-07-17 | 2018-07-11 | Novozymes A/S | Pullulanase chimeras and polynucleotides encoding same |
| CN105934518A (zh) | 2013-09-11 | 2016-09-07 | 诺维信公司 | 用于生产发酵产品的方法 |
| EP3097192B1 (en) | 2014-01-22 | 2018-07-25 | Novozymes A/S | Pullulanase variants and polynucleotides encoding same |
| WO2016062875A2 (en) | 2014-10-23 | 2016-04-28 | Novozymes A/S | Glucoamylase variants and polynucleotides encoding same |
| CN104480127B (zh) * | 2014-12-14 | 2017-04-05 | 长春中医药大学 | 超嗜热糖苷酶突变体及其在人参皂苷ck制备中的应用 |
| WO2016196202A1 (en) | 2015-05-29 | 2016-12-08 | Novozymes A/S | Polypeptides having protease activity and polynucleotides encoding same |
| CA2981342A1 (en) | 2015-06-18 | 2016-12-22 | Novozymes A/S | Polypeptides having trehalase activity and the use thereof in process of producing fermentation products |
| WO2017015329A1 (en) | 2015-07-23 | 2017-01-26 | Novozymes A/S | Alpha-amylase variants and polynucleotides encoding same |
| US10494685B2 (en) * | 2015-10-14 | 2019-12-03 | Novozymes A/S | Glucoamylase variants and polynucleotides encoding same |
| US10597645B2 (en) | 2015-12-22 | 2020-03-24 | Novozymes A/S | Process of extracting oil from thin stillage |
| CN110072997A (zh) | 2016-11-23 | 2019-07-30 | 诺维信公司 | 具有蛋白酶活性的多肽和编码其的多核苷酸 |
| US10889836B2 (en) | 2016-11-23 | 2021-01-12 | Novozymes A/S | Yeast for ethanol production |
| CA3055530A1 (en) * | 2017-03-07 | 2018-09-13 | Danisco Us Inc. | Thermostable glucoamylase and methods of use, thereof |
| CN110730824A (zh) | 2017-06-02 | 2020-01-24 | 诺维信公司 | 用于乙醇生产的改善的酵母 |
| US11584783B2 (en) | 2017-06-28 | 2023-02-21 | Novozymes A/S | Processes for producing a fermentation product |
| US11220679B2 (en) | 2017-08-08 | 2022-01-11 | Novozymes A/S | Polypeptides having trehalase activity |
| CA3070730A1 (en) | 2017-09-15 | 2019-03-21 | Novozymes A/S | Enzyme blends and processes for improving the nutritional quality of animal feed |
| CN111148830A (zh) | 2017-10-04 | 2020-05-12 | 诺维信公司 | 具有蛋白酶活性的多肽和编码其的多核苷酸 |
| CA3075907A1 (en) | 2017-10-23 | 2019-05-02 | Novozymes A/S | Processes for reducing lactic acid in a biofuel fermentation system |
| CN107828855B (zh) * | 2017-10-31 | 2020-08-21 | 南京欣迪生物药业工程有限责任公司 | 一种精浆中性α-葡糖苷酶检测试剂盒及其应用 |
| DK3720955T5 (da) | 2017-12-08 | 2024-08-26 | Novozymes As | Alfa-amylasevarianter og polynukleotider, der koder for dem |
| BR112020011173A2 (pt) * | 2017-12-08 | 2020-11-17 | Novozymes A/S | variante de alfa-amilase, polinucleotídeo, composição, construto de ácido nucleico, vetor de expressão, célula hospedeira, método de produção de uma variante de alfa-amilase, uso da variante, e, processo para produção de um xarope a partir de material contendo amido. |
| EP3746545A1 (en) | 2018-01-29 | 2020-12-09 | Novozymes A/S | Microorganisms with improved nitrogen utilization for ethanol production |
| AU2019222480B9 (en) | 2018-02-15 | 2024-11-28 | Microbiogen Pty. Ltd. | Improved yeast for ethanol production |
| WO2019197318A1 (en) | 2018-04-09 | 2019-10-17 | Novozymes A/S | Polypeptides having alpha-amylase activity and polynucleotides encoding same |
| WO2019231944A2 (en) | 2018-05-31 | 2019-12-05 | Novozymes A/S | Processes for enhancing yeast growth and productivity |
| US20210269833A1 (en) | 2018-07-11 | 2021-09-02 | Novozymes A/S | Processes for producing fermentation products |
| MX2021000893A (es) | 2018-07-25 | 2021-03-31 | Novozymes As | Levadura que expresa enzimas para la producción de etanol. |
| CN113286889A (zh) | 2018-10-08 | 2021-08-20 | 诺维信公司 | 用于生产乙醇的表达酶的酵母 |
| BR112021014873A2 (pt) | 2019-01-31 | 2021-10-05 | Novozymes A/S | Polipeptídeo, combinação de enzimas, polinucleotídeo, construto de ácido nucleico ou vetor de expressão recombinante, célula hospedeira recombinante, método de produção de um polipeptídeo, e, processo de produção de um produto de fermentação |
| BR112021017085A2 (pt) | 2019-04-02 | 2022-02-08 | Novozymes As | Processo de produção de um produto de fermentação |
| WO2021021458A1 (en) | 2019-07-26 | 2021-02-04 | Novozymes A/S | Microorganisms with improved nitrogen transport for ethanol production |
| WO2021026201A1 (en) | 2019-08-05 | 2021-02-11 | Novozymes A/S | Enzyme blends and processes for producing a high protein feed ingredient from a whole stillage byproduct |
| MX2021015818A (es) | 2019-08-06 | 2022-02-03 | Novozymes As | Proteinas de fusion para mejorar la expresion de enzimas. |
| CA3158982A1 (en) | 2019-12-10 | 2021-06-17 | Monica TASSONE | Microorganism for improved pentose fermentation |
| PY2084892A (es) | 2019-12-16 | 2022-07-26 | Novozymes As | Proceso para producir productos de fermentación |
| US20230142991A1 (en) | 2020-02-10 | 2023-05-11 | Novozymes A/S | Alpha-amylase variants and polynucleotides encoding same |
| CN116438309A (zh) * | 2020-11-02 | 2023-07-14 | 诺维信公司 | 葡糖淀粉酶变体和编码它们的多核苷酸 |
| CN112359027B (zh) * | 2021-01-13 | 2021-04-13 | 凯莱英生命科学技术(天津)有限公司 | 细胞色素p450酶突变体及其应用 |
| CN115386506A (zh) * | 2021-04-16 | 2022-11-25 | 中国科学院亚热带农业生态研究所 | 促进畜禽粪便和秸秆快速堆肥的微生物复合菌剂及其生产生物有机肥的方法 |
| AU2022288057A1 (en) | 2021-06-07 | 2023-12-21 | Novozymes A/S | Engineered microorganism for improved ethanol fermentation |
| WO2023288234A1 (en) * | 2021-07-12 | 2023-01-19 | Xylogenics, Inc. | Strains of saccharomyces cerevisiae that exhibit an increased ability to ferment oligosaccharides into ethanol without supplemental glucoamylase and methods of making and using the same |
| CN113881654B (zh) * | 2021-09-08 | 2023-06-09 | 暨南大学 | 一种对胃蛋白酶抗性提高的α-淀粉酶 |
| EP4638768A2 (en) | 2022-12-19 | 2025-10-29 | Novozymes A/S | Processes for producing fermentation products using fiber-degrading enzymes with engineered yeast |
| JP2025541374A (ja) | 2022-12-19 | 2025-12-18 | ノボザイムス アクティーゼルスカブ | アラビノフラノシダーゼ及びキシラナーゼを含む組成物、並びにヘミセルロース系繊維の可溶化を増加させるためのその使用 |
| EP4638724A1 (en) | 2022-12-19 | 2025-10-29 | Novozymes A/S | Carbohydrate esterase family 1 (ce1) polypeptides having ferulic acid esterase and/or acetyl xylan esterase activity and polynucleotides encoding same |
| WO2024137252A1 (en) | 2022-12-19 | 2024-06-27 | Novozymes A/S | Process for reducing syrup viscosity in the backend of a process for producing a fermentation product |
| EP4638725A1 (en) | 2022-12-19 | 2025-10-29 | Novozymes A/S | Carbohydrate esterase family 3 (ce3) polypeptides having acetyl xylan esterase activity and polynucleotides encoding same |
| WO2024258820A2 (en) | 2023-06-13 | 2024-12-19 | Novozymes A/S | Processes for producing fermentation products using engineered yeast expressing a beta-xylosidase |
| WO2025128568A1 (en) | 2023-12-11 | 2025-06-19 | Novozymes A/S | Composition and use thereof for increasing hemicellulosic fiber solubilization |
| WO2026008449A2 (en) | 2024-07-04 | 2026-01-08 | Novozymes A/S | A process for producing a fermentation product and a concentrated protein co-product |
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| WO2000034452A1 (en) * | 1998-12-07 | 2000-06-15 | Novozymes A/S | Glucoamylases with n-terminal extensions |
| WO2011127802A1 (en) * | 2010-04-14 | 2011-10-20 | Novozymes A/S | Polypeptides having glucoamylase activity and polynucleotides encoding same |
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- 2012-10-11 MX MX2014004292A patent/MX351762B/es active IP Right Grant
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- 2012-10-11 DK DK12775472.9T patent/DK2766476T3/en active
- 2012-10-11 WO PCT/EP2012/070127 patent/WO2013053801A1/en not_active Ceased
- 2012-10-11 EA EA201490649A patent/EA201490649A1/ru unknown
- 2012-10-11 EP EP12775472.9A patent/EP2766476B1/en active Active
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| US20250236859A1 (en) | 2025-07-24 |
| EP2766476B1 (en) | 2017-05-31 |
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| US20210062172A1 (en) | 2021-03-04 |
| US10113158B2 (en) | 2018-10-30 |
| MX2014004292A (es) | 2014-07-24 |
| IN2014CN03468A (https=) | 2015-07-03 |
| US20190002856A1 (en) | 2019-01-03 |
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| US20240084277A1 (en) | 2024-03-14 |
| US9540624B2 (en) | 2017-01-10 |
| US10865399B2 (en) | 2020-12-15 |
| MX351762B (es) | 2017-10-26 |
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