CN103893700A - Chinese medicinal composition for treating breast cancer - Google Patents
Chinese medicinal composition for treating breast cancer Download PDFInfo
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Abstract
The invention belongs to the field of medicine, in particular to a Chinese medicinal composition for treating breast cancer and a preparation method thereof. The Chinese medicinal composition is prepared from the following bulk pharmaceuticals in parts by weight: 15-35 parts of pseudo-ginseng root, 10-20 parts of sargentg loryvine stem, 15-35 parts of red-rooted salvia root, 15-25 parts of frankincense, 10-20 parts of common burreed rhizome, 15-25 parts of astragalus, 5-15 parts of common cephalanoplos herb, 15-25 parts of medicinal cyathula root, 5-15 parts of indigowoad leaf, 5-15 parts of peach kernel, 5-15 parts of common selfheal fruit-spike, 10-20 parts of skunk bugbane, 5-15 parts of evening primrose, 5-15 parts of plantain herb, 10-20 parts of concha arcae, 5-15 parts of white paeony root, 10-20 parts of nutgrass galingale rhizome, 10-20 parts of turmeric root-tuber, 5-15 parts of medicinal indianmulberry root and 10-20 parts of dendrobium. The Chinese medicinal composition has the advantages of remarkable treatment effect on breast cancer, freeness from toxic and side effects and prevention of relapse after healing, is up to 90 percent in effective rate, and is an ideal medicament for treating breast cancer.
Description
Technical field
The invention belongs to field of medicaments, be specifically related to a kind of Chinese medicine composition for the treatment of breast carcinoma and preparation method thereof.
Background technology
Breast carcinoma is one of modal malignant tumor of women, and according to statistics, sickness rate accounts for the 7-10% of the various malignant tumor of whole body, is that one has a strong impact on the even life-threatening disease of women's physical and mental health.Breast carcinoma refers to that breast duct epithelial cell is under the effect of various inside and outside carcinogenic factors, and cell loses normal characteristic and paraplasm, so that exceedes the limit of self-regeneration and the disease of canceration occurs.Its performance be the earliest suffer from breast occur single-shot, relatively circular, oval or difform little lump of form, quality is hard, indefinite border generally; A few patients can have distending pain, dull pain.The morbidity of breast carcinoma is normal relevant with heredity, and between 40-60 year, menopause front and back women's sickness rate higher.The cause of disease of breast carcinoma is also completely not clear and definite, but the factors such as bearing status, oral contraceptive, inherited genetic factors, ionizing radiation, unsound dietary habit have been identified with breast cancer related.Especially the long-term factor such as dietary structure, living habit causes the excessive acidify of body constitution, the function of human body integral declines, cause that the liver of suffering from a deficiency of the kidney is also empty, and then cause that part of the body cavity above the diaphragm housing the heart and lungs metabolic cycles is slack-off, easily cause thyroid disease and endocrine disturbance, immunologic function degression, thereby develop into mammary gland tissue paraplasm, whole carcinogenesis.
The method of existing treatment breast carcinoma is a lot, mainly contains operative treatment, chemotherapy and Drug therapy, and these therapies are all undesirable.Mammary cancer surgery can cause damage and the QI and blood loss of patient's body tissue, and patient is had a delicate constitution, and easily causes the recurrence of cancer, diffusion and the transfer of cancerous cell.Chemotherapy is one of common method of breast carcinoma non-operative treatment, but the wretched insufficiencies such as it exists, and cancerous cell is killed together with normal cell, effective dose and toxic dose approach very much, toxic and side effects, wherein wait reaction the most obvious so that the impaired and hemopoietic function of bone marrow of digestive function is suppressed, often make patient with breast cancer seriously be difficult to accept chemotherapy and maybe can not adhere to the whole course for the treatment of because reacting.
The breast carcinoma traditional Chinese medical science is referred to as breast carcinoma, have another name called milk rock etc., its pathogeny be mainly in positive QI-insufficiency, wind and cold, invade, internal injury caused by excess of seven emotions, be willing to unsuccessful, pent-up impairing the liver, cause internal organs dysfunction in body, Chong and Ren channel disorder, reduce the supervision resistance of body to cancerous cell, oncogene effect and body's immunity cause eventually when low qi stagnation blood stasis, evil poison accumulate interior, the turbid friendship of expectorant solidifying, tie stagnant newborn portion and form.Chinese medicine is the exclusive medical advantage of China, and Chinese medicine, in treatment breast carcinoma, not only can promote physical recovery, strengthens immunologic function, and can bring into play antitumaous effect, prevents recurrence and shifts, and improves survival rate.Therefore, develop and develop effective Chinese medicine preparation and treat breast carcinoma, the hygiene medical treatment cause tool of China is of great significance.The inventor gropes by a large amount of experiments, has found a kind of Chinese medicine composition for the treatment of breast carcinoma, and its pharmacodynamics and clinical experiment effect are very remarkable.
Summary of the invention
The present invention is from the rule for the treatment of of Chinese traditional treatment breast carcinoma, through development test, by Radix Notoginseng, Caulis Sargentodoxae, Radix Salviae Miltiorrhizae, Olibanum, Rhizoma Sparganii, the Radix Astragali, Herba Cirsii, Radix Cyathulae, Folium Isatidis, Semen Persicae, Spica Prunellae, Rhizoma Cimicifugae, Radix Oenotherae erythrosepalae, Herba Plantaginis, Concha Arcae, the Radix Paeoniae Alba, Rhizoma Cyperi, Radix Curcumae, Radix Morindae Officinalis, Herba Dendrobii 20 taste medicines, through suitable processing, extraction, make a kind of Chinese medicine composition of effective treatment breast carcinoma.
On the one hand, the invention provides a kind of Chinese medicine composition for the treatment of breast carcinoma, its raw material by following weight parts is prepared from: Radix Notoginseng 15-35 part, Caulis Sargentodoxae 10-20 part, Radix Salviae Miltiorrhizae 15-35 part, Olibanum 15-25 part, Rhizoma Sparganii 10-20 part, Radix Astragali 15-25 part, Herba Cirsii 5-15 part, Radix Cyathulae 15-25 part, Folium Isatidis 5-15 part, Semen Persicae 5-15 part, Spica Prunellae 5-15 part, Rhizoma Cimicifugae 10-20 part, Radix Oenotherae erythrosepalae 5-15 part, Herba Plantaginis 5-15 part, Concha Arcae 10-20 part, Radix Paeoniae Alba 5-15 part, Rhizoma Cyperi 10-20 part, Radix Curcumae 10-20 part, Radix Morindae Officinalis 5-15 part, Herba Dendrobii 10-20 part.
In a preferred embodiment, described Chinese medicine composition is preferably formed by the Chinese drug preparation of following weight portion: 25 parts of Radix Notoginseng, 15 parts of Caulis Sargentodoxae, 25 parts of Radix Salviae Miltiorrhizaes, 20 parts of Olibanums, 15 parts of Rhizoma Sparganii, 20 parts of the Radixs Astragali, 10 parts of Herba Cirsiis, 20 parts of Radix Cyathulaes, 10 parts of Folium Isatidiss, 10 parts, Semen Persicae, 10 parts of Spica Prunellaes, 15 parts of Rhizoma Cimicifugaes, 10 parts of Radix Oenotherae erythrosepalaes, 10 parts of Herba Plantaginiss, 15 parts of Concha Arcaes, 10 parts of the Radix Paeoniae Albas, 15 parts of Rhizoma Cyperis, 15 parts of Radix Curcumaes, 10 parts of Radix Morindae Officinaliss, 15 parts of Herba Dendrobiis.
On the other hand, the invention provides the preparation method of described Chinese medicine composition, it comprises the steps:
(1) according to proportioning weighting raw materials Radix Notoginseng, Caulis Sargentodoxae, Radix Salviae Miltiorrhizae, Olibanum, Rhizoma Sparganii, the Radix Astragali, Herba Cirsii, Radix Cyathulae, Folium Isatidis, Semen Persicae, Spica Prunellae, Rhizoma Cimicifugae, Radix Oenotherae erythrosepalae, Herba Plantaginis, Concha Arcae, the Radix Paeoniae Alba, Rhizoma Cyperi, Radix Curcumae, Radix Morindae Officinalis and the Herba Dendrobii of above-mentioned Chinese medicine composition, pulverize mixing for standby use;
(2) crude drug in step (1) was soaked in water to (water did not have powder 3-8cm) after 30-60 minute, intense fire boils, 30-45 minute at a simmer, cooling, filter after obtain filtrate and medicinal residues;
(3) medicinal residues that obtain in step (2) are decocted with water, amount of water was not to have medicinal residues face 2-5cm, and intense fire boils, 15-30 minute at a simmer, cooling, filter after obtain filtrate and filtering residue;
(4) twice filtrate of merging obtain the Chinese medicine composition of described treatment breast carcinoma.
Further preferably, the preparation method of Chinese medicine composition of the present invention comprises the steps:
(1) according to proportioning weighting raw materials Radix Notoginseng, Caulis Sargentodoxae, Radix Salviae Miltiorrhizae, Olibanum, Rhizoma Sparganii, the Radix Astragali, Herba Cirsii, Radix Cyathulae, Folium Isatidis, Semen Persicae, Spica Prunellae, Rhizoma Cimicifugae, Radix Oenotherae erythrosepalae, Herba Plantaginis, Concha Arcae, the Radix Paeoniae Alba, Rhizoma Cyperi, Radix Curcumae, Radix Morindae Officinalis and the Herba Dendrobii of above-mentioned Chinese medicine composition, pulverizing, mixing for standby use;
(2) crude drug in step (1) was soaked in water to (water did not have powder 5cm) after 45 minutes, intense fire boils, 40 minutes at a simmer, cooling, filter after obtain filtrate and medicinal residues;
(3) medicinal residues that obtain in step (2) are decocted with water, amount of water was not to have medicinal residues face 5cm, and intense fire boils, 25 minutes at a simmer, cooling, filter after obtain filtrate and filtering residue;
(4) twice filtrate of merging obtain the Chinese medicine composition of described treatment breast carcinoma.
In a preferred embodiment, can, according to the preparation method of this area routine, by adding appropriate adjuvant, Chinese medicine composition of the present invention be made to the preparations such as tablet, granule, capsule, soft extract, pill, medicinal tea, powder, drop pill.
Described adjuvant comprises field of medicaments conventional and general adjuvant in the above-mentioned preparation process of preparation.
On the one hand, the present invention also provides the medical usage of described Chinese medicine composition again.The instructions of taking of Chinese medicine composition of the present invention: be decocted in water for oral dose, 1 dose of every day, a point morning and evening takes for twice, and serveing on 30 days is a course for the treatment of.Clinical and pharmacological experiment result shows, traditional Chinese medicine composition for treating breast carcinoma successful of the present invention, has no side effect, and is difficult for more afterwards recurrence, and effective percentage reaches 95%.Open up new road for the treatment of breast carcinoma clinically, there is important social value.
In Chinese medicine composition of the present invention, the meridian distribution of property and flavor of each taste Chinese medicine and effect thereof are as follows:
Radix Notoginseng, warm in nature, acrid in the mouth.There is dissipating blood stasis hemostasis, effect of subduing swelling and relieving pain.Cure mainly spitting of blood, spit blood, epistaxis, has blood in stool, metrorrhagia, and traumatic hemorrhage, breast ventral spine pain, tumbling down swells and ache.
Caulis Sargentodoxae, nature and flavor hardship, flat.There is the eliminating carbuncle of relieving internal heat, promoting blood circulation to remove obstruction in the collateral, the effect of wind dispelling insecticide.Dispelling the wind and dampness pathogens arthralgia pain, bloody dysentery, stranguria with blood, menoxenia, infantile malnutrition, worm pain, injury from falling down.
Radix Salviae Miltiorrhizae, bitter in the mouth, feeble QI is cold, nontoxic.Enter the heart, spleen two warps.There is effect of promoting blood circulation to restore menstrual flow, evacuation of pus granulation promoting, specially regulating menstruation arteries and veins, reason iliacus is ached, and tissue regeneration promoting blood, removes stagnant blood.
Olibanum, arduous, temperature.Enter the heart, liver, spleen channel.Have regulating qi and activating blood, analgesic therapy, chases after effect of poison.Control coagulation of QI-blood, trusted subordinate's pain, carbuncle pyogenic infections from tumour or sore, traumatic injury, dysmenorrhea, postnatal blood stasis twinge.
Rhizoma Sparganii, acrid in the mouth, hardship, property is flat.Enter liver, spleen channel.Have that blood stasis dispelling stimulates the menstrual flow, effect of removing blood stasis eliminating mass, circulation of qi promoting removing food stagnancy.
The Radix Astragali, sweet, tepor.Return lung, spleen, liver, kidney channel.There is tonifying Qi and lifting yang, strengthening superficial resistance to stop perspiration, line water detumescence, effect of promoting pus discharge and tissue regeneration strengthening.
Herba Cirsii, sweet, cool, enter liver, spleen channel.There is effect of removing heat from blood, blood stasis dispelling, hemostasis.Treatment haematemesis, epistaxis, hematuria, stranguria with blood, have blood in stool, metrorrhagia, acute infectious hepatitis, wound hemorrhage, furuncle, carbuncle poison.
Radix Cyathulae, sweet, micro-hardship, flat.Return liver, kidney channel.There is eliminating blood stasis and inducing menstruation, easing joint movement, effect of inducing diuresis for treating stranguria syndrome.For amenorrhea lump in the abdomen, retention of placenta, arthralgia, flaccidity of the lower limbs clonic spasm of the muscle, hematuria stranguria with blood, injury from falling down.
Folium Isatidis, hardship, cold.Enter liver, the heart, stomach warp.Heat clearing away, removing toxic substances, removing heat from blood, hemostasis.Treatment epidemic febrile disease intenseness of heat excessive thirst, influenza, acute infectious hepatitis, bacillary dysentery, acute gastroenteritis, acute pneumonia, erysipelas, haematemesis, epistaxis, jaundice, dysentery, sore throat, aphtha, swollen ulcer drug.
Semen Persicae, bitter, sweet, flat.GUIXIN, liver, large intestine channel.Blood circulation promoting and blood stasis dispelling, loosening bowel to relieve constipation, relieving cough and asthma.For amenorrhea, dysmenorrhea, WEIJIA mass in the abdomen, injury from falling down, dryness of the intestine constipation.
Spica Prunellae, cold in nature, sweet in the mouth, pungent, micro-hardship, there is the effect of letting out clearly liver-fire, mass dissipating and swelling eliminating, heat-clearing and toxic substances removing, expelling phlegm for arresting cough, cooling blood for hemostasis, be applicable to lymphoid tuberculosis, goiter, acute mastitis, the vertigo, facial hemiparalysis, bones and muscles pain, pulmonary tuberculosis, metrorrhagia, leukorrhagia, Fiedeler's disease type hepatitis and bacillary dysentery etc.
Rhizoma Cimicifugae, yang invigorating, deliver, rash, removing toxic substances.Control that seasonal epidemic pathogens epidemic disease, headache cold and heat, miliaria pruritus due to heat, laryngalgia, burst toxic swelling, gastric heat toothache, dispute skin ulcer, macule are saturating, sinking of QI of middle-JIAO, chronic diarrhea chronic dysentery, proctoptosis, women collapse, uterus drops, carbuncle sore tumefacting virus.
Radix Oenotherae erythrosepalae, bitter in the mouth, micro-pungent, micro-sweet, property is flat.Promoting blood circulation to remove obstruction in the collateral, endogenous wind stopping suppressing the hyperactive liver, detumescence sore.Cure mainly how moving obstruction of qi in the chest and cardialgia, apoplectic hemiplegia, stirring-up of pathogenic wind in the interior resulting from deficiency, children's be, wind dampness rheumatic anesthesia pain, stomachache are had loose bowels, dysmenorrhea, oral-anal-genital ulcer syndrome, skin infection, eczema.
Herba Plantaginis, sweet, cold.Return liver, kidney, lung, small intestine meridian.Clearing away heat and promoting diuresis, removing heat from blood, removing toxic substances.Cure mainly accumulation of heat in the urinary bladder, dysuria, stranguria with turbid discharge leukorrhagia, heat-damp in summer dysentery, epistaxis, hematuria, liver-heat conjunctival congestion, laryngopharynx swelling and pain, carbuncle sore tumefacting virus.
Concha Arcae, reduces phlegm, softening the hard mass, dissipating blood stasis, removing food stagnancy.Cure mainly accumulation of phlegm, stomachache, acid regurgitation, scrofula, ulcerative gingivitis.
The Radix Paeoniae Alba, cool in nature, bitter in the mouth is sour, be slightly cold.There is the easing the affected liver of enriching blood, suppressing the hyperactive liver pain relieving, yin fluid astringing and receive the effects such as antiperspirant, be applicable to fever due to yin deficiency, menoxenia, the breast abdomen pain over the hypochondriac region, extremity contraction anxious, dysentery stomachache, spontaneous sweating, metrorrhagia, be with inferior disease.
Rhizoma Cyperi, pungent micro-hardship is sweet, flat.Enter liver, tri-jiao channel.There is the resolving depression of regulating the flow of vital energy, menstruction regulating and pain relieving, the effect such as antiabortive.Cure mainly that distending pain over the hypochondrium, mammary swelling are educated, hernia pain, menoxenia, gastral cavity abdomen feeling of fullness pain, belch acid regurgitation vomiting and nausea, abdominal pain during menstruation, bleeding not during menses, frequent fetal movement.
Radix Curcumae, acrid in the mouth, hardship, cold in nature.Return liver, the heart, lung meridian.There is the use of regulating qi to disperse stagnation, heart fire-clearing upset-relieving, promoting the function of the gallbladder to alleviate jaundice, promoting blood circulation and stopping pain, promoting QI circulation for relieving depression, the removing heat from blood that clears away heart-fire.
Radix Morindae Officinalis, acrid in the mouth, sweet; Slightly warm in nature.Enter spleen, kidney two warps.Reinforcing the kidney and supporting YANG; Bone and muscle strengthening; Expelling wind and removing dampness.Cure mainly: impotence due to deficiency of the kidney; Seminal emission premature ejaculation; Cold and pain in the lower abdomen; Incontinence of urine; Cold womb is infertile; Anemofrigid-damp arthralgia; Soreness of the waist and knees; Rheumatism limb gas.
Herba Dendrobii, is slightly cold, sweet, returns stomach, kidney channel.There is effect of reinforcing stomach reg fluid, nourishing YIN and clearing away heat.For cloudy impairment of body fluid lose, xerostomia excessive thirst, lack of appetite are retched, the disease such as deficiency-heat, poor vision after being ill.
Beneficial effect of the present invention:
The present invention is based on the traditional Chinese medical science treatment breast carcinoma is carried out to the dialectical principle treating, and breast carcinoma belongs to the category such as " newborn painful abdominal mass ", " breast carcinoma " of Chinese medicine, and is a kind of systemic disease, is the local clinical manifestation of general disease.The various crude drug of the present invention coordinate can farthest bring into play drug effect mutually together, play altogether the merit of kidney tonifying tonify deficiency, nourishing blood and promoting blood circulation blood stasis dispelling, removing obstruction in the collateral to relieve pain, tumor suppression eliminating stagnation, treating both the principal and secondary aspects of a disease, can improve immunity of human body itself, nervous system regulation, and Chinese medicine composition of the present invention to human body do not produce any toxic side effects, safe and effective.Therefore, the present invention provides a kind of safe, effective for prior art, and the Chinese medicine composition that is used for the treatment of breast carcinoma with low cost, preparation technology is simple and easy to do.
The specific embodiment
Further describe technical scheme of the present invention by following embodiment.It is pointed out that following explanation is only illustrating of the technical scheme claimed to the present invention, the not any restriction to these technical schemes.The content that protection scope of the present invention is recorded with appended claims is as the criterion.
embodiment 1
Treat a Chinese medicine composition for breast carcinoma, its crude drug by following weight is prepared from:
Radix Notoginseng 25g, Caulis Sargentodoxae 15g, Radix Salviae Miltiorrhizae 25g, Olibanum 20g, Rhizoma Sparganii 15g, Radix Astragali 20g, Herba Cirsii 10g, Radix Cyathulae 20g, Folium Isatidis 10g, Semen Persicae 10g, Spica Prunellae 10g, Rhizoma Cimicifugae 15g, Radix Oenotherae erythrosepalae 10g, Herba Plantaginis 10g, Concha Arcae 15g, Radix Paeoniae Alba 10g, Rhizoma Cyperi 15g, Radix Curcumae 15g, Radix Morindae Officinalis 10g, Herba Dendrobii 15g.
The preparation of described Chinese medicine composition and instructions of taking are: get the crude drug of above-mentioned weight, pulverizing, mix homogeneously, be decocted in water for oral dose, and potion, divided and sooner or later took for twice every day, within one month, was a course for the treatment of.
The preparation method of described Chinese medicine composition, concrete steps are as follows:
(1) according to proportioning weighting raw materials Radix Notoginseng, Caulis Sargentodoxae, Radix Salviae Miltiorrhizae, Olibanum, Rhizoma Sparganii, the Radix Astragali, Herba Cirsii, Radix Cyathulae, Folium Isatidis, Semen Persicae, Spica Prunellae, Rhizoma Cimicifugae, Radix Oenotherae erythrosepalae, Herba Plantaginis, Concha Arcae, the Radix Paeoniae Alba, Rhizoma Cyperi, Radix Curcumae, Radix Morindae Officinalis and the Herba Dendrobii of above-mentioned Chinese medicine composition, pulverizing, mixing for standby use;
(2) crude drug in step (1) was soaked in water to (water covers powder 5cm) after 45 minutes, intense fire boils, 40 minutes at a simmer, cooling, filter after obtain filtrate and medicinal residues;
(3) medicinal residues that obtain in step (2) are decocted with water, amount of water was not to have medicinal residues face 5cm, and intense fire boils, 25 minutes at a simmer, cooling, filter after obtain filtrate and filtering residue;
(4) twice filtrate of merging obtain the Chinese medicine composition of described treatment breast carcinoma.
embodiment 2
Treat a Chinese medicine composition for breast carcinoma, its crude drug by following weight is prepared from:
Radix Notoginseng 35g, Caulis Sargentodoxae 10g, Radix Salviae Miltiorrhizae 35g, Olibanum 15g, Rhizoma Sparganii 20g, Radix Astragali 15g, Herba Cirsii 15g, Radix Cyathulae 15g, Folium Isatidis 15g, Semen Persicae 5g, Spica Prunellae 15g, Rhizoma Cimicifugae 10g, Radix Oenotherae erythrosepalae 15g, Herba Plantaginis 5g, Concha Arcae 20g, Radix Paeoniae Alba 5g, Rhizoma Cyperi 20g, Radix Curcumae 10g, Radix Morindae Officinalis 15g, Herba Dendrobii 10g.
The preparation of described Chinese medicine composition and instructions of taking are: get the crude drug of above-mentioned weight, pulverizing, mix homogeneously, be decocted in water for oral dose, and potion, divided and sooner or later took for twice every day, within one month, was a course for the treatment of.
The preparation method of described Chinese medicine composition, concrete steps are as follows:
(1) according to proportioning weighting raw materials Radix Notoginseng, Caulis Sargentodoxae, Radix Salviae Miltiorrhizae, Olibanum, Rhizoma Sparganii, the Radix Astragali, Herba Cirsii, Radix Cyathulae, Folium Isatidis, Semen Persicae, Spica Prunellae, Rhizoma Cimicifugae, Radix Oenotherae erythrosepalae, Herba Plantaginis, Concha Arcae, the Radix Paeoniae Alba, Rhizoma Cyperi, Radix Curcumae, Radix Morindae Officinalis and the Herba Dendrobii of above-mentioned Chinese medicine composition, pulverizing, mixing for standby use;
(2) crude drug in step (1) was soaked in water to (water covers powder 8cm) after 60 minutes, intense fire boils, 45 minutes at a simmer, cooling, filter after obtain filtrate and medicinal residues;
(3) medicinal residues that obtain in step (2) are decocted with water, amount of water was not to have medicinal residues face 3cm, and intense fire boils, 20 minutes at a simmer, cooling, filter after obtain filtrate and filtering residue;
(4) twice filtrate of merging obtain the Chinese medicine composition of described treatment breast carcinoma.
embodiment 3
Treat a Chinese medicine composition for breast carcinoma, its crude drug by following weight is prepared from:
Radix Notoginseng 15g, Caulis Sargentodoxae 20g, Radix Salviae Miltiorrhizae 15g, Olibanum 25g, Rhizoma Sparganii 10g, Radix Astragali 25g, Herba Cirsii 5g, Radix Cyathulae 25g, Folium Isatidis 5g, Semen Persicae 15g, Spica Prunellae 5g, Rhizoma Cimicifugae 20g, Radix Oenotherae erythrosepalae 5g, Herba Plantaginis 15g, Concha Arcae 10g, Radix Paeoniae Alba 15g, Rhizoma Cyperi 10g, Radix Curcumae 20g, Radix Morindae Officinalis 5g, Herba Dendrobii 20g.
The preparation of described Chinese medicine composition and instructions of taking are: get the crude drug of above-mentioned weight, pulverizing, mix homogeneously, be decocted in water for oral dose, and potion, divided and sooner or later took for twice every day, within one month, was a course for the treatment of.
The preparation method of described Chinese medicine composition, concrete steps are as follows:
(1) according to proportioning weighting raw materials Radix Notoginseng, Caulis Sargentodoxae, Radix Salviae Miltiorrhizae, Olibanum, Rhizoma Sparganii, the Radix Astragali, Herba Cirsii, Radix Cyathulae, Folium Isatidis, Semen Persicae, Spica Prunellae, Rhizoma Cimicifugae, Radix Oenotherae erythrosepalae, Herba Plantaginis, Concha Arcae, the Radix Paeoniae Alba, Rhizoma Cyperi, Radix Curcumae, Radix Morindae Officinalis and the Herba Dendrobii of above-mentioned Chinese medicine composition, pulverizing, mixing for standby use;
(2) crude drug in step (1) was soaked in water to (water covers powder 3cm) after 30 minutes, intense fire boils, 30 minutes at a simmer, cooling, filter after obtain filtrate and medicinal residues;
(3) medicinal residues that obtain in step (2) are decocted with water, amount of water was not to have medicinal residues face 2cm, and intense fire boils, 15 minutes at a simmer, cooling, filter after obtain filtrate and filtering residue;
(4) twice filtrate of merging obtain the Chinese medicine composition of described treatment breast carcinoma.
the anticancer experiment in vitro of embodiment 4 Chinese medicine compositions of the present invention
Adopt classical cytotoxic activity vitro detection method mtt assay, the cell proliferation toxicity of the human tumor cells of test sample to In vitro culture.
When experiment, sample adds DMSO, is diluted to desired concn by RPMI1640 culture medium, ultrasonic, is partly dissolved, and sample segment solution is suspension, with nutritional solution successively doubling dilution.
Cell strain: MCF-7 Breast Cancer Cell.
Experimental technique:
Experiment adopts conventional MTT detection method.Inoculating cell: the trophophase cell of taking the logarithm, after 0.25% trypsinization, add the RPMI-1640 culture fluid that contains 10% embryo Ox blood serum, cell dilution is become to single cell suspension, adjust cell number, be inoculated in 96 orifice plates, every hole 100 μ L.Postvaccinal cell is put in 37 ℃, 5% CO2 gas incubator and cultivated 24 hours.Dosing: tested medicine is carried out to doubling dilution with RPMI-1640 culture fluid, configure altogether 6 concentration.In 96 orifice plates, add survey product liquid, 100 μ L/ holes, each sample concentration is established 4 multiple holes, and matched group is established 5 multiple holes, and sample concentration is 1 × 10
-4mol/L.Matched group is supplied residual volume with the RPMI-1640 culture fluid containing 10% hyclone, and making reaction volume is 200 μ L/ holes.After application of sample, cell is put in 37 ℃, 5% CO2 gas incubator and continued to cultivate 68 hours.Add MTT: abandon supernatant, add MTT5mg/ml, dissolve with PBS, every hole adds 10 μ L.Put 37 ℃ and continue to cultivate taking-up after 4 hours.Survey OD value: the supernatant in reject 96 orifice plates, every hole adds DMSO 150 μ L.Measure OD value at microplate reader 570nm, calculate inhibitory rate of cell growth with following formula:
Suppression ratio=[(the average OD value of the average OD value/matched group of 1-medication group)] × 100%
Experimental result:
The inhibitory action of Chinese medicine composition prepared by the embodiment 1-3 MCF-7 Breast Cancer Cell to In vitro culture is as shown in the table:
Table 1
| Embodiment | MCF-7 suppression ratio |
| 1 | 87% |
| 2 | 84% |
| 3 | 80% |
According to above in vitro tests result, Chinese medicine composition prepared by embodiment of the present invention 1-3 all has significant inhibitory action to MCF-7 Breast Cancer Cell, and its suppression ratio reaches more than 80%.
the inhibitory action of embodiment 5 Chinese medicine composition of the present invention to transplanted breast carcinoma
1, laboratory animal and tumor strain
Female Sexual health TA2 mice, body weight 18-22g, Mus 6-8 in age week, purchased from zoopery center, institute of oncology of Jiangsu Province; Mouse mastopathy cell strain MA-891 is purchased from zoopery center, institute of oncology of Jiangsu Province.
2, modeling and experimental technique
60 of TA2 mices, stay 10 negative matched groups in proportion at random, and all the other 50 are carried out modeling.The exponential phase MA-891 mouse mastopathy cell of getting In vitro culture, cell concentration is adjusted into l × 10
7/ ml.Under aseptic condition, MA-891 mouse mastopathy cell is inoculated in to the right armpit of TA2 mice subcutaneous, inoculum concentration is that only (cell number is 2 × 10 to 0.2ml/
6/ only).Whole seeded process must be carried out with sterile working in aseptic cover, completes inoculation in 1h.
After inoculation, be divided at random 6 groups, 10 every group, be respectively tumor model matched group, Chinese medicine composition group (embodiment 1-3), 5-fluorouracil (5-Fu) positive controls and negative control group.After inoculation oncocyte 24h, test group lumbar injection Chinese medicine composition, dosage is 20mg/kg, negative control group and tumor model matched group equal-volume injectable drug solvent, 5-Fu matched group is take normal saline as solvent, dosage is 20mg/kg, each treated animal all by 0.2ml at lumbar injection, successive administration 10d.Next day after last administration, observe as follows:
Completely peel off tumor piece, and the calculating tumor control rate of weighing, tumor control rate=(matched group tumor weight-treatment group tumor weight)/matched group tumor weight × 100%
3, experimental result:
Chinese medicine composition prepared by the embodiment 1-3 inhibitory action to Implanted breast cancer in mouse: 20mg/kg Chinese medicine composition can significantly suppress the growth (P< 0.01) of Implanted breast cancer in mouse, and its anti-breast cancer activity is higher than chemotherapeutic 5-fluorouracil.In table 2.
Table 2
| Group | Dosage | Number of animals | Tumor weight (x ± s) | Tumour inhibiting rate (%) | P value |
| Model control group | ? | 10 | 3.47±0.11 | ? | <0.01 |
| 5-Fu | 20mg/kg | 10 | 1.38±0.14 | 60.23% | <0.01 |
| Embodiment 1 | 20mg/kg | 10 | 1.29±0.17 | 62.82% | <0.01 |
| Embodiment 2 | 20mg/kg | 10 | 1.34±0.18 | 61.38% | <0.01 |
| Embodiment 3 | 20mg/kg | 10 | 1.40±0.12 | 59.65% | <0.01 |
the Clinical observation of embodiment 6 Chinese medicine compositions of the present invention
Be selected from inpatient's totally 120 examples with complete data.Be divided at random treatment group 60 examples, matched group 60 examples.The treatment group age is 35-60 year, and 47.5 years old mean age, the matched group age, 45.5 years old mean age, the age was without statistically-significant difference (P > 0.05) in 32-59 year.
Diagnostic criteria:
Western medicine diagnose standard: touch lump in breast, matter is hard, touch less than obvious lump, but cancerous cell has been found in pathology or cytolgical examination in air spots or breast, all can be diagnosed as breast carcinoma.
Tcm diagnosis standard:
Syndrome Differentiation of Traditional Chinese Medicine
1. caused by hepatic stagnation qi stagnation: see lump in breast, matter is hard, and the colour of skin is constant; Feelings will is not smooth, vexed poor appetite, feeling of oppression over the chest and distention of hypochondrium, distention and pain of breast before menstruation, tongue dark yellow fur, stringy pulse or stringy and thready pulse.
2. spleen emptiness and wet sputum type: breast caking, matter is firmly uneven, and there is core oxter, shallow complexion, spiritlessness and weakness, gastral cavity uncomfortable in chest is swollen, anorexia loose stool, pale tongue has indentation, and tongue is greasy in vain, and rolling pulse is thin.
3. Chong and Ren channel disorder type: common through thing disorder, premenstrual distending pain of the breast, above the average age for marriage unmarried or after marriage not fertility or fertility too much, or repeatedly miscarriage, or not suckling in puerperal, lump in breast is hard, pale tongue with thin fur, stringy and thready pulse.
4. resistance type in ecchymosis: there is piece in Ruzhong, and quality is hard, scorching hot pain, the colour of skin is purple dark, and boundary is unclear, and what push away is motionless, or lump ulceration, seepage flow blood or yellow fluid, the smelly pain of taste; Unhappy irritability, it is poor that headache is slept, xerostomia happiness drink, dry stool yellowish urine, purplish tongue is dark, or has ecchymosis, and yellow fur is thick and dry, deep and hesitant pulse or stringy and rapid pulse.
5. the two types that lose of QI and blood: there is piece in Ruzhong, are uneven, seemingly as heap foxtail millet, first rotten rear routed, and Tianjin when sewage is hemorrhage smelly, dizzy margins of eyelids, and heart beating is breathed hard, waist soreness, hyperhidrosis is slept poor, urinates clear loose stool, pale tongue with white fur, deep-thready pulse.
Therapeutic Method:
Treatment group is taken the Chinese medicine composition of embodiment 1, and every day, potion, took at twice sooner or later, and be 30 days a course for the treatment of, takes continuously two courses for the treatment of.
Matched group adopts CMF scheme, CTX-cyclophosphamide; MTX-methotrexate; 5-fluorouracil (5-Fu).CTX600 milligram, quiet note.MTX15 milligram, quiet note.5-Fu500 milligram, vein drips past.First inject MTX, after 6 hours, inject again CTX and 5-Fu, to obtain drug synergism.Every 7-10 days administration 1 time, treats two months.
Efficacy assessment standard:
Draft chemotherapeutic efficacy standard with reference to national breast carcinoma meeting in 1987:
1. (CR) entirely disappears: all cancer of seeing of looking into disappear completely, and osteolytic lesion is all calcifications on x-ray;
2. part disappears (PR): can measure pathological changes and dwindle 50% (products of 2 maximum perpendicular diameters of axle), occur simultaneously, but do not require that all focuses all belong to PR without new focus.
3. stable without becoming (S): tumor is dwindled < 50%, or increases < 25%.
4. worsen (PD): some focus is dwindled < 50%, some focus increases > 25% simultaneously, or the increase of all focuses, or has new focus to occur.
Therapeutic outcome: two groups of therapeutic outcomes are as shown in table 3:
Table 3
| Group | Number | CR/ example | PR/ example | S/ example | PD/ example | Effective percentage |
| Treatment group | 60 | 23 | 22 | 12 | 3 | 95% |
| Matched group | 60 | 17 | 14 | 14 | 15 | 75.0% |
Through X 2 test, two groups of effective percentage comparisons, difference has very significant (P < 0.01)
model case
Single certain, 47 years old.Find lump in breast at Hospital Physical Examination the year before, without obvious pain, also aperiodicity changes, and quality is hard as rock, and mobility is poor.Be right breast carcinoma through hospital diagnosis, patient's emaciated physique, dim complexion, fatigue and weakness.Disease is seen through thing disorder, premenstrual distending pain of the breast, and lump in breast is hard, pale tongue, white and thin fur, stringy and thready pulse.1 course for the treatment of of Chinese medicine composition of taking the embodiment of the present invention 1, the premenstrual distending pain of the breast of patient alleviates to some extent, and sleep improves.2 courses for the treatment of of Chinese medicine composition of the following the service embodiment of the present invention 1, the premenstrual distending pain of the breast of patient disappears, and menstrual cycle is normal, and lump in breast before dwindles.1 course for the treatment of of following the service Chinese medicine composition of the present invention, lump in breast disappears, and all diseases are all removed, and examination in hospital is without difference.Follow up a case by regular visits to 1 year, health is good.
Zhu, 50 years old, an approximately 3 × 3.5cm of lump was found in the outer top of left breast, and matter is hard, rough, irregular around, and hospital diagnosis is breast carcinoma.Disease is seen left arm distending pain, and slightly sense is numb sometimes, shallow complexion, tired mind, thin and greasy fur, stringy pulse.Take 1 course for the treatment of of Chinese medicine composition of the embodiment of the present invention 1, patient's appetite increases, and sleep improves, and all diseases all have alleviation.2 courses for the treatment of of following the service Chinese medicine composition of the present invention, the left swelling of the breast piece of patient dwindles, and left arm distending pain, pain and numbness disappear.1 course for the treatment of of following the service Chinese medicine composition of the present invention, after treatment, abnormal all diseases disappear substantially, and the index of tumor is all normal.Follow up a case by regular visits to 1 year, health is good.
Zhao so-and-so, 38 years old, the existing lump of 2 years front left suppurative mastitis, was breast carcinoma through hospital diagnosis, patient takes two courses for the treatment of of Chinese medicine composition of the embodiment of the present invention 1, lump progressively diminishes, and continues to take medicine after 2 courses for the treatment of, lump disappears completely, examination in hospital is without difference.Follow up a case by regular visits to 1 year, health is good.
Content of the present invention only for example understands some claimed specific embodiments; the technical characterictic of recording in one of them or more technical scheme can be with one or more technical schemes be combined arbitrarily; these technical schemes that obtain through combination are also in the application's protection domain, just as these technical schemes that obtain through combination have specifically been recorded in the disclosure of invention.
Claims (7)
1. treat a Chinese medicine composition for breast carcinoma, its crude drug by following weight portion is prepared from: Radix Notoginseng 15-35 part, Caulis Sargentodoxae 10-20 part, Radix Salviae Miltiorrhizae 15-35 part, Olibanum 15-25 part, Rhizoma Sparganii 10-20 part, Radix Astragali 15-25 part, Herba Cirsii 5-15 part, Radix Cyathulae 15-25 part, Folium Isatidis 5-15 part, Semen Persicae 5-15 part, Spica Prunellae 5-15 part, Rhizoma Cimicifugae 10-20 part, Radix Oenotherae erythrosepalae 5-15 part, Herba Plantaginis 5-15 part, Concha Arcae 10-20 part, Radix Paeoniae Alba 5-15 part, Rhizoma Cyperi 10-20 part, Radix Curcumae 10-20 part, Radix Morindae Officinalis 5-15 part, Herba Dendrobii 10-20 part.
2. Chinese medicine composition according to claim 1, it is preferably prepared from by the crude drug of following weight portion: 25 parts of Radix Notoginseng, 15 parts of Caulis Sargentodoxae, 25 parts of Radix Salviae Miltiorrhizaes, 20 parts of Olibanums, 15 parts of Rhizoma Sparganii, 20 parts of the Radixs Astragali, 10 parts of Herba Cirsiis, 20 parts of Radix Cyathulaes, 10 parts of Folium Isatidiss, 10 parts, Semen Persicae, 10 parts of Spica Prunellaes, 15 parts of Rhizoma Cimicifugaes, 10 parts of Radix Oenotherae erythrosepalaes, 10 parts of Herba Plantaginiss, 15 parts of Concha Arcaes, 10 parts of the Radix Paeoniae Albas, 15 parts of Rhizoma Cyperis, 15 parts of Radix Curcumaes, 10 parts of Radix Morindae Officinaliss, 15 parts of Herba Dendrobiis.
3. a preparation method for the Chinese medicine composition described in claim 1-2, it comprises the steps:
(1) according to proportioning weighting raw materials Radix Notoginseng, Caulis Sargentodoxae, Radix Salviae Miltiorrhizae, Olibanum, Rhizoma Sparganii, the Radix Astragali, Herba Cirsii, Radix Cyathulae, Folium Isatidis, Semen Persicae, Spica Prunellae, Rhizoma Cimicifugae, Radix Oenotherae erythrosepalae, Herba Plantaginis, Concha Arcae, the Radix Paeoniae Alba, Rhizoma Cyperi, Radix Curcumae, Radix Morindae Officinalis and the Herba Dendrobii of Chinese medicine composition, pulverize mixing for standby use;
(2) crude drug in step (1) was soaked in water to (water did not have powder 3-8cm) after 30-60 minute, intense fire boils, 30-45 minute at a simmer, cooling, filter after obtain filtrate and medicinal residues;
(3) medicinal residues that obtain in step (2) are decocted with water, amount of water did not have medicinal residues face 2-5cm, and intense fire boils, 15-30 minute at a simmer, cooling, filter after obtain filtrate and filtering residue;
(4) merge twice filtrate and obtain the Chinese medicine composition for the treatment of breast carcinoma.
4. the preparation method of Chinese medicine composition according to claim 3, its step comprises:
(1) according to proportioning weighting raw materials Radix Notoginseng, Caulis Sargentodoxae, Radix Salviae Miltiorrhizae, Olibanum, Rhizoma Sparganii, the Radix Astragali, Herba Cirsii, Radix Cyathulae, Folium Isatidis, Semen Persicae, Spica Prunellae, Rhizoma Cimicifugae, Radix Oenotherae erythrosepalae, Herba Plantaginis, Concha Arcae, the Radix Paeoniae Alba, Rhizoma Cyperi, Radix Curcumae, Radix Morindae Officinalis and the Herba Dendrobii of Chinese medicine composition, pulverizing, mixing for standby use;
(2) crude drug in step (1) was soaked in water to (water did not have powder 5cm) after 45 minutes, intense fire boils, 40 minutes at a simmer, cooling, filter after obtain filtrate and medicinal residues;
(3) medicinal residues that obtain in step (2) are decocted with water, amount of water did not have medicinal residues face 5cm, and intense fire boils, 25 minutes at a simmer, cooling, filter after obtain filtrate and filtering residue;
(4) twice filtrate of merging obtain the Chinese medicine composition of described treatment breast carcinoma.
5. a pharmaceutical preparation that contains the Chinese medicine composition described in claim 1-2, it is characterized in that, described Chinese medicine composition and pharmaceutically acceptable adjuvant are prepared into suitable pharmaceutical preparation, such as tablet, granule, capsule, soft extract, pill, medicinal tea, powder, drop pill etc.
6. pharmaceutical preparation according to claim 5, is characterized in that, described pharmaceutical preparation is preferably tablet or granule.
7. the application of the Chinese medicine composition described in claim 1-2 in the medicine of preparation treatment breast carcinoma.
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| CN105999076A (en) * | 2016-06-29 | 2016-10-12 | 江苏苏南药业实业有限公司 | Compound traditional Chinese medicine composition for treating breast cancer and preparation method thereof |
| CN108635512A (en) * | 2018-07-17 | 2018-10-12 | 霍山县天下泽雨生物科技发展有限公司 | A kind of detection method of Dendrobidium huoshanness refined polysaccharide antitumor activity |
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| CN101322818A (en) * | 2008-07-25 | 2008-12-17 | 刘扬 | Chinese medicine for treating galactophore lobular hyperplasia |
| CN101564521A (en) * | 2009-05-20 | 2009-10-28 | 石振刚 | External plaster for treating hyperplasia of mammary glands |
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| CN105999076A (en) * | 2016-06-29 | 2016-10-12 | 江苏苏南药业实业有限公司 | Compound traditional Chinese medicine composition for treating breast cancer and preparation method thereof |
| CN108635512A (en) * | 2018-07-17 | 2018-10-12 | 霍山县天下泽雨生物科技发展有限公司 | A kind of detection method of Dendrobidium huoshanness refined polysaccharide antitumor activity |
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