CN103891680A - Sucrose industrial waste processing method through ecological transformation of houseflies - Google Patents
Sucrose industrial waste processing method through ecological transformation of houseflies Download PDFInfo
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Abstract
The invention discloses a sucrose industrial waste processing method through ecological transformation of houseflies. The method comprises the steps of fly collection and clean feeding and domestication, bagasse conversion to adapt to induction and stabilization of flies, seed selection of cellulase high-expression flies, induction of cellulase and lipase double-high-expression flies, preprocessing of biotransformation of fly maggots of sucrose industrial waste, stabilization of industrial bagasse converted flies, and production of fly maggot industrial products. Compared with the prior art, the biotechnology is adopted for sucrose industrial waste ecological transformation function induction and screening, passage and engineering breeding of the houseflies, the mechanical disruption and microbial fermentation technology is combined, the modern engineering technology is adopted, the resource comprehensive treatment of the sucrose industrial waste taking the biological fermentation and insect metabolism conversion as the main is achieved, and the method has the advantages of being high in ecological degree, large in bioavailability, short in rotten cycle, high in product quality, high in additional value of resources, free of environmental pollution, scientific in ratio, high in fertilizer efficiency and the like.
Description
Technical field
The present invention relates to the method for the ecological conversion processing industrial sucrose of a kind of housefly discarded object, belong to housefly cultivation and industrial sucrose waste utilization technical field.
Background technology
Sugar industry is the basic industries of food service industry, is again the primary industry of the multiple products such as papermaking, chemical industry, fermentation, medicine, building materials, furniture, occupies critical role in national economy.Take Guangxi as example, sugar industry is as the pillar industry of Economy in Guangxi.Total 63Ge county of the Guangxi whole district grows cane, and population concerning farmers is nearly 2,000 ten thousand, within 2009/2010 year, squeezes season sugarcane grower and plants sugarcane income and reach more than 200 hundred million yuan, and sugaring and comprehensive utilization income from sales thereof reach 47,800,000,000 yuan; Within 2009/2010 year, squeeze season, 7,120,000 tons of Guangxi sugar output, account for 66.4% of national sugar output, and Guangxi sugar output accounts for the more than 50% of national gross yield in continuous 10 years, are maximum sugaring bases, the whole nation.The huge economic benefit of bringing to Guangxi as main industry taking candy paper binding pattern, nearly 10,000,000,000 yuan of annual value of production.
The discarded object producing in cane sugar manufacture industrial processes mainly contains three kinds of bagasse, molasses and filter muds.The comprehensive benefit have begun to take shape that Chinese Sugar Industry recycling economy produces at present, its industry endless form mainly contains: (1) sugarcane-sugaring-molasses-alcohol-alcohol effluent-composite fertilizer-also field kind sugarcane; (2) reclaim-recycling of sugarcane-sugaring-bagasse-pulping and paper-making-waste liquid alkali; (3) sugarcane-sugaring-molasses-yeast, monosodium glutamate-waste liquid-composite fertilizer-also field kind sugarcane; (4) sugarcane-sugaring-bagasse-generating-bagasse ash-fertilizer-also field kind sugarcane; (5) sugarcane-sugaring-filter mud-composite fertilizer-also field kind sugarcane.
Although sugar industry recycling economy has obtained great achievement, but still face multiple bottleneck, restricted the performance of recycling economy potentiality.The subject matter existing at present has:
(1) sugaring comprehensive utilization ratio is still on the low side, and part discarded object is not fully used, and for example: the industrialized utilization of sugarcane leaf and the sugarcane tip also rests on the starting stage, the filter mud that carbonatation process sugar refining technology produces still cannot all utilize; Some Enterprises is restricted by scale, and the resources such as its bagasse, molasses are not fully used.
(2) the environmental protection treatment high expensive of portioned product comprehensive utilization, processes not thoroughly, and product competitiveness is not strong, not high with the agriculture degree of association; For example: because scale is less than normal, the pollution intractability producing in the production technology of bagasse papermaking and molasses-spirit is larger, 1 ton of paper of every production need to produce 30-40 ton waste water, unit product environmental protection treatment high expensive.
Therefore, in conjunction with current actual conditions, seek efficient, Environmental security, resource and make full use of the technology of industrial sucrose discarded object, become organic connections industrial and agricultural development, form pollution-free industry chain, support comprehensively cane planting sustainable development, ensure the inexorable trend of Guangxi pillar industry development.
Summary of the invention
The object of the invention is to: the method that the ecological conversion processing industrial sucrose of a kind of housefly discarded object is provided.The present invention adopt biotechnology carry out the ecological transformation function induction of housefly industrial sucrose discarded object and screen, go down to posterity, through engineering approaches raising, in conjunction with Mechanical Crushing, microbial fermentation technology, adopt modernization project means, realizing the industrial sucrose changing rejected material to useful resource that microorganism is fermented, insect metabolic conversion is master administers, pollution-free in production technology, near-zero release; The method can a large amount of industrial sucrose discarded object of safe and efficient quick resource on the spot integrated treatment, and products obtained therefrom source (functional) organic fertilizer, animal health-care feed and biomedical raw material or product can be applied to the aspects such as plant husbandry, livestock breeding, fishery and Bio-pharmaceutical Industry.
Technical scheme of the present invention: the method for the ecological conversion processing industrial sucrose of a kind of housefly discarded object, it comprises the following steps:
(1) fly kind gathers and clean raising domestication: collect the wild housefly in the sucrose place of production, become fly to raise with milk powder, sugar, larva cleans cultivation with wheat bran, starts nature go down to posterity more than 5 generations from larva, makes it have stable reproductive characteristic;
(2) bagasse transforms the induction that adapts to fly strain with stable: adopt mechanical crushing to 200-800 order bagasse, destroy lignin structure, then add wheat bran and water to be mixed with the bagasse composts or fertilisers of cultivating of water content 50%-90%, under environmental temperature 20-30 DEG C, the condition of relative moisture 50%-80%, the common house-fly grub after step (1) domestication is cultivated in induction; The proportioning of inductivity bagasse composts or fertilisers of cultivating is from bagasse powder: wheat bran=1: 9 are increased to bagasse powder: wheat bran=5:5 gradually, and each proportioning ratio is cultivated 5-10 generation; Again from bagasse powder: wheat bran=5:5 is increased to bagasse powder: wheat bran=6:4(is because bagasse nutrition is inadequate, continuing increase can cause fly maggot not grow by normal growth, therefore be only increased to 6:4), each proportioning ratio is cultivated 10-15 generation, make housefly life respectively go through phase biological property and reach nature or wheat bran raising growth standard, obtain thus bagasse and transform the strain of adaptation fly;
(3) seed selection of cellulase high expressed fly strain: the larva that bagasse is transformed to adaptation fly strain inoculation is placed in bagasse powder: wheat bran=6: 4 bagasse composts or fertilisers of cultivating is bred, random packet, in per generation, gets in composts or fertilisers of cultivating crude fiber content and measures minimum group and pupate into fly, adopt the method for genetic screening to obtain the fly strain of secretion cellulase high expressed, after the crude fiber content in composts or fertilisers of cultivating is lower than 15%, raise again 3-5 generation, obtain stable cellulase high expressed fly strain;
(4) induction of cellulase, the two high expressed fly strains of lipase: the high fiber high glucose and high fat fat environment that the larva of cellulase high expressed fly strain is placed in to 60% bagasse, 20% wheat bran, 5% lard, 5% sucrose and 10% albumen auxiliary material composition is bred, circulation 10-15 obtains stable cellulase, the two high expressed fly strains of lipase after generation;
(5) industrial sucrose discarded object fly maggot biological transformation pretreatment: bagasse mechanical crushing, to 200-800 order, is 10 by concentration
6-10
8(because the content of lignin of bagasse is very high, the whiterot fungi concentration therefore using should not be lower than 10 for the whiterot fungi culture fluid of/ml
6/ ml), water, bagasse powder, then mixes with the bagasse powder that do not ferment the bagasse powder after activation than admixture activation 8 hours by the quality of 1: 1: 20 by the mass ratio of 1: 500, the opening 1-3 days that ferments, obtains using modified bagasse powder; 1:0.8-1.2 adds pH adjusting agent in molasses by weight proportion, and pH value is adjusted to 6.5-7.5; Filter mud is heated to 70-80 DEG C, with isopyknic water flushing, removes cerosin wherein; Then count by weight percentage, get bagasse powder 40%-60%, molasses 10%-15%, filter mud 10%-20%, wheat bran or cavings 10%-15%, albumen auxiliary material 8%-10% after modification, and regulate water content to 65%-75%, be made into fly maggot suitability for industrialized production bed material, make it reach the standard of industrialization fly-maggot feed;
(6) industrialization bagasse transforms the stable of fly strain: the larva of cellulase, the two high expressed fly strains of lipase is placed in to fly maggot suitability for industrialized production bed material and breeds, circulation 10-15 obtains stable industrialization bagasse and transforms fly strain after generation, transform fly strain carry out the production of fly maggot industrialization product with this;
(7) production of fly maggot industrialization product: the remaining bed material after maggot grow is dried and made source (functional) organic fertilizer; Common house-fly grub with become fly to make animal health-care feed or adopt biotechnology processing to make biomedical raw material or product.
More particularly, preceding method comprises the following steps:
(1) fly kind gathers and clean raising domestication: collect the wild housefly in the sucrose place of production, carry out Biology identification, then become fly to raise with the mass ratio milk powder of 1: 1, sugar, larva is with the clean cultivation of wheat bran, start nature from larva and go down to posterity more than 5 generations, make it under intensive feeding environment, there is stable reproductive characteristic;
(2) bagasse transforms the induction that adapts to fly strain with stable: adopt mechanical crushing to 400 order (owing to containing a large amount of lignin in bagasse bagasse, therefore be crushed to higher than clasmatosis level 320 orders, 400 orders all can above, must there is special equipment but 800 orders are above, cost costliness, therefore be crushed to 400 order left and right), destroy lignin structure, then add wheat bran and water to be mixed with the bagasse composts or fertilisers of cultivating of water content 50%-90%, under environmental temperature 20-30 DEG C, the condition of relative moisture 50%-80%, the common house-fly grub after step (1) domestication is cultivated in induction; The proportioning of inductivity bagasse composts or fertilisers of cultivating is from bagasse powder: wheat bran=1: 9 are increased to bagasse powder: wheat bran=5:5 gradually, each proportioning ratio is cultivated 5-10 generation, and each culture process is that the 3 day old larva → fly → milk powder of pupating → sprout wings into is cultivated → separated in the induction of fly blow → bagasse composts or fertilisers of cultivating, sugar is raised and laid eggs; Again from bagasse powder: wheat bran=5:5 is increased to bagasse powder: wheat bran=6:4, each proportioning ratio is cultivated 10-15 generation, and each culture process is that the 4 day old larva → fly → milk powder of pupating → sprout wings into is cultivated → separated in the induction of fly blow → bagasse composts or fertilisers of cultivating, sugar is raised and laid eggs; Make housefly life respectively go through phase biological property and reach nature or wheat bran raising growth standard, obtain thus bagasse and transform the strain of adaptation fly;
(3) seed selection of cellulase high expressed fly strain: the larva that bagasse is transformed to adaptation fly strain inoculation is placed in bagasse powder: wheat bran=6: 4 bagasse composts or fertilisers of cultivating is bred, random packet, in per generation, gets in composts or fertilisers of cultivating crude fiber content and measures minimum group and pupate into fly, adopt the method for genetic screening to obtain the fly strain of secretion cellulase high expressed, after the crude fiber content in composts or fertilisers of cultivating is lower than 15%, raise again 3-5 generation, obtain stable cellulase high expressed fly strain;
(4) induction of cellulase, the two high expressed fly strains of lipase: the high fiber high glucose and high fat fat environment that the larva of cellulase high expressed fly strain is placed in to 60% bagasse, 20% wheat bran, 5% lard, 5% sucrose and 10% albumen auxiliary material composition is bred, process of growth is that fly blow → inoculation bed material → separation 4 day old larva → fly → milk powder of pupating → sprout wings into, sugar raising are laid eggs; Circulation 10-15 obtains stable cellulase, the two high expressed fly strains of lipase after generation;
(5) industrial sucrose discarded object fly maggot biological transformation pretreatment: bagasse mechanical crushing to 400 order is 10 by concentration
6the whiterot fungi culture fluid of/ml, water, bagasse powder, then mix with the bagasse powder that do not ferment the bagasse powder after activation than admixture activation 8 hours by the quality of 1: 1: 20 by the mass ratio of 1: 500, the opening 1-3 days that ferments, obtains using modified bagasse powder; 1:0.8-1.2 adds pH adjusting agent in molasses by weight proportion, and pH value is adjusted to 6.5-7.5; Filter mud is heated to 70-80 DEG C, with isopyknic water flushing, removes cerosin (water after flushing reclaims and extracts cerosin) wherein; Then count by weight percentage, get bagasse powder 40%, molasses 15%, filter mud 20%, wheat bran or cavings 15%, albumen auxiliary material 10% after modification, and regulate water content to 65%-75%, and be made into fly maggot suitability for industrialized production bed material, make it reach the standard of industrialization fly-maggot feed;
(6) industrialization bagasse transforms the stable of fly strain: the larva of cellulase, the two high expressed fly strains of lipase is placed in to fly maggot suitability for industrialized production bed material and breeds, process of growth is that fly blow → inoculation bed material → separation 4 day old larva → fly → milk powder of pupating → sprout wings into, sugar raising are laid eggs; Circulation 10-15 obtains stable industrialization bagasse and transforms fly strain after generation, transform fly strain carry out the production of fly maggot industrialization product with this;
(7) production of fly maggot industrialization product: the remaining bed material after maggot grow is dried and made source (functional) organic fertilizer; Common house-fly grub with become fly to make animal health-care feed or adopt biotechnology processing to make biomedical raw material or product.
In preceding method, due to the Tamed and led process more complicated of industrialization bagasse conversion fly strain, therefore the anti-degeneration to fly strain, can not directly introduce wild housefly, but stable cellulase after continual introducing Tamed and led, the two high expressed fly strains of lipase, in step (6), transform fly strain every 20 days to industrialization bagasse according to the spawning peak time span of housefly and introduce stable cellulase, the two high expressed fly strains of lipase, because the new housefly sprouting wings does not lay eggs for first 4 days, and the too many stable cellulase of introducing, the two high expressed fly strain meetings of lipase reduce the egg laying amount in the unit interval, therefore stable in order to maintain population, the quantity of introducing is each every cage 3%-5%.
In preceding method, the filter mud described in step (5) is sulfurous method filter mud or carbonatation process filter mud.
In preceding method, because contain a certain amount of calcium carbonate in filter mud, so carbonatation process filter mud is before preparation fly maggot suitability for industrialized production bed material, 1:1 adds weakly acidic organic waste water in mass ratio, described weakly acidic organic waste water can be the slurry of cleaning equipment in fly maggot suitability for industrialized production, can be also sanitary wastewater.
In preceding method, the pH adjusting agent in step (5) is sodium bicarbonate or lime stone.
In preceding method, step (4), (5) described albumen auxiliary material are dry maggot powder, corn flour, colza cake or dregs of beans.
In preceding method, the proportioning of inductivity bagasse composts or fertilisers of cultivating is from bagasse powder in step (2): wheat bran=1: 9 are increased to bagasse powder gradually: wheat bran=5:5 process, every generation is selected in the 3 age in days fly maggot larvas immigration wheat bran that action is active, build is larger and is pupated into fly, until survive, the each polypide quality of fly maggot reaches more than 25 milligrams, then enters next proportioning ratio; Inductivity bagasse composts or fertilisers of cultivating proportioning is from bagasse powder: wheat bran=5:5 is increased to bagasse powder: wheat bran=6:4 process, every generation is selected in the 4 age in days fly maggot larvas immigration wheat bran that action is active, build is larger and is pupated into fly, in the time that the each polypide quality of survival fly maggot reaches more than 25 milligrams, in this proportioning ratio, continue to cultivate 3-5 generation, make fly strain each side physiological situation reach stable, then enter next proportioning ratio.
In preceding method, step (1), (2) and (4) described domestication, induction, the wild housefly in locality is carried out to the engineering culture adaptability inducing and acclimating under this specific feeding environment of bagasse, make the common house-fly grub can normal growth when intensive farm in bagasse, reach every 150 grams of fly maggots of 10 kilograms of bagasse composts or fertilisers of cultivating breeding, the each polypide quality of 3 age in days fly maggot more than 25 milligrams, fully transform the organic resource in industrial sucrose discarded object.
In preceding method, stable industrialization bagasse described in step (6) transforms fly strain and refers to common house-fly grub growth and breeding in the fly maggot suitability for industrialized production bed material of using modified bagasse powder preparation, reaches every 10 kilograms of bed materials and cultivates at least 350 grams of output larvas, each polypide quality more than 25 milligrams.
The patent No. is to have recorded a kind of method that is converted into the main quick intensive improvement of stalk with housefly metabolism ecology in 201210299214.4 patent document, and the present invention selects bagasse, and the difference of bagasse and other stalks is in table 1.
Table 1
As seen from the above table, compared with other stalks, the content of lignin of bagasse is higher, and nutrient component (ash content+crude protein) is low especially, and fly maggot can not directly be grown in bagasse.Through experimental study, in bagasse, add the amount of whiterot fungi than the many orders of magnitude of the stalk of same weight, pulverizing rank will be higher than stalk, needs the nutrient component of interpolation more.And molasses and filter mud are the accessory substances of cane sugar manufacture industry, be convenient to collect, can be used as nutrition accessory and add in bagasse.
Molasses be by the juice from sugar cane squeezing out through heating, neutralization, precipitation, filter, remaining dense thick liquid after the operation such as concentrated, crystallization, it is raw material common in animal feed, also be a kind of high-quality feed ingredient that has much characteristic, can increase the nutritive value of feed and improve the growth performance of animal.Its ingredient and content are in table 2.
The composition of table 2 molasses
| Trophic level | Content |
| Dry matter content DM | 75.44 |
| Crude protein content CP | 9.79 |
| Coarse ash content Ash | 12.0 |
| Sugar Sugar | 55.40 |
| Potassium K | 6.71 |
| Nitrogen N | 3.0 |
| Phosphorus P | 0.08 |
| Magnesium Mg | 0.23 |
| Copper Cu | ≤10.0mg/kg |
| Acidity Acid Dip | ≤15 |
| Sodium Na | 2.23 |
| Calcium Ca | 0.4 |
| Thiamine | 0.0005 |
| Vitamin b3 | 0.00012 |
| Pyridoxine | 0.0009 |
| Folic acid | 0.00002 |
| Vitamin h | 0.00015 |
| Pantothenic acid | 0.007 |
| Vitamin PP | 0.0015 |
| Total plate count cfu | 5.0×10 5 |
In cane sugar manufacture industry, the lipides of most cane stalks (cerosin and sugarcane fat) total amount is 0.2%～0.3%, and sugarcane is through the multiple squeezing in sugar refinery, and total lipides wherein approximately have 60% to residue in bagasse, and 40% enters mixed juice.In cane sugar manufacture production process, because the non-sugar organic substances such as the contained protein of mixed juice, pectic substance, organic acid are unfavorable to cane sugar boiling and crystal, therefore, must carry out clarifying treatment to mixed juice, add the materials such as slaked lime, sulphur dioxide, phosphoric acid to mixed juice, the non-sugar organic matters such as protein, pectic substance, organic acid are precipitated, the filter residue that sediment obtains by filter press or vacuum suction filter or centrifugal separation equipment, be called filter mud, it is one of large by product of cane sugar manufacture.
The component of filter mud because of different production technologies different.Sulfurous method filter mud because of ash load few, filter mud is not containing a large amount of calcium salts, in dry, total amount of organic can reach 80% left and right, be mainly lipides (cerosin and sugarcane fat) 5%～14%, fiber 15%～30%, sugar 5%～15%, crude protein 5%～15%, total ash 9%～20%, wherein be rich in the required various mineral nutritions of sugarcane production, pH6.0 left and right.And in carbonatation process filter mud dry containing CaCO
3amount reaches 70%～80% left and right, and composition is taking inorganic matter as main, and content of organics is 15% left and right, band alkalescence, pH9.0 left and right.Carbonatation process filter mud, sulfurous method filter mud main chemical compositions are in table 3.
The composition of the different sugar refining technology filter muds of table 3
| Process | pH | N | P 2O 5 | K 2O | CaO | MgO | Moisture |
| ? | ? | (%) | (%) | (%) | (%) | (%) | (%) |
| Sulfurous method | 5.95 | 1.19 | 1.24 | 0.52 | 5.59 | 1.07 | 69.55 |
| Carbonatation process | 8.97 | 0.69 | 0.65 | 0.27 | 35.97 | 4.72 | 55.91 |
Therefore in the filter mud that bagasse and sulfurous method extract, all contain a certain amount of cerosin and sugarcane fat, cerosin and sugarcane fat are water insoluble, these lipid materials and protein have very strong adhesion simultaneously, and in conjunction with very firm, be unfavorable for the absorption of fly maggot to nutriment in bed material, even can be combined by the secretion in fly maggot body surface or enteron aisle, on fly maggot body surface and intestines wall, form a skim, thereby cause the death of fly maggot.In addition, research also show: the digestive ferment of housefly has amylase, protease, lipase, taking amylase as main, other enzymic activity a little less than.Therefore must again induce the strain of existing cellulase high expressed fly, improve the content of lipase in its digestive tract and body surface secretion, to decompose cerosin and the sugarcane fat in filter mud.
Two orthogonal experiment primary conditions below: each size is 0.8-1.2m
3fly cage in put into 300-400 gram of fly pupa, fly cage is placed in airtight lightweight steel construction color steel room, every 1.5-2m
3space, steel plate room in place a fly cage; Ten thousand of the about 3.0-3.4 of one-tenth fly after every cage fly pupa is sprouted wings, male and female half and half; 28 DEG C of temperature, humidity 60%, illumination every day 8 hours, the adult flies breeding feed 2g/ cage that adds per hour.
High fiber high glucose and high fat fat environment of the present invention (i.e. 60% bagasse, 20% wheat bran, 5% lard, 5% sucrose, 10% albumen auxiliary material) is that applicant studies, screens in conjunction with the high glucose and high fat fat feed of raising mouse, and the positive quadraturing design test of its three factors, five levels is in table 4.
Table 4
According to experimental result, the 13rd group and above 12 groups statistically there is no notable difference, consider the formula of the 13rd group most suitable as the formulation material of high fiber high glucose and high fat fat environment fly maggot breeding.
Applicant is also optimized screening to the proportioning of fly maggot suitability for industrialized production bed material, and its orthogonal experiment is in table 5.
Table 5
Above-mentioned result of the test shows: with the bagasse 40%-60% that fills a prescription, molasses 10%-15%, filter mud 10-20%, remainder is that wheat bran and albumen auxiliary material are raised fly maggot, its egg laying amount of sprouting wings into after fly all can reach industrialization production requirements, egg laying amount is between 500000-540000, and wherein experimental group 5 eclosion rates are up to the most applicable formula.
Compared with prior art, the present invention is by inducing housefly and screening, go down to posterity, through engineering approaches is raised, and ecological integrity Mechanical Crushing, microbial fermentation technology, adopt modernization project means, it is main realizing insect processing metabolism, the industrial sucrose changing rejected material to useful resource that microorganism is fermented collaborative is administered, directly link industrial sucrose, by industrial sucrose discarded object---bagasse, molasses, filter mud fully utilizes, solve the offal treatment problem that sucrose squeezes factory, and make efficient, clean, the green organic fertilizer of environmental protection and housefly polypide protein product and the medicine of high added value, chemical raw material product.And production process is pollution-free, to the production of the construction of ecological organic agricultural chain, organic agriculture by product, have very important significance.
In " 12 national strategy new industry development plan ", clearly propose: biological industry will be towards great demands such as people ' s health, agricultural development, protections of natural resources and environment; the Key Common Technologies such as the enhanced biological utilization of resources and technological equipment exploitation, accelerate to build modern biological industry system.Therefore, promoting the present invention has very important significance.On the one hand, the organic waste resource that can utilize more fully sugar industry to produce, process on the spot, use on the spot, linking secure agro-product processing simultaneously and agricultural production, by industrial sucrose discarded object and agricultural planting---the cane planting of safely cleaning combines, ensureing the sustainable development of industrial sucrose, set up the enclosed ecological agriculture, is a kind of important means that realizes agricultural modernization; On the other hand; promote the present invention; the insect resources that development of new functional organic fertilizer and FAO (Food and Agriculture Organization of the United Nation) advocate, realizes top grade, the high value added utilization of the sugar industry organic waste raw materials such as low-quality bagasse, for protection agroecological environment provides an effective way.
Simultaneously; the present invention meets national agricultural industry policy; Guangxi pillar industry sugar industry organic waste is raised for housefly engineering; integrate multiple biotechnology; the organic wastes such as bagasse are made to the very green organic fertilizers of high-quality; it has advantages of organic, green, environmental protection, efficient, safety, control plant pest, improves sugarcane and other quality of agricultural product; can realize organic also field simultaneously, improve soil property, the soil of fostering and apply fertilizer, reduce heavy metal pollution of soil etc., ecotope is had to protective effect.All products, by product in fly maggot raising all have high added value, and production process is pollution-free, to in-depth agricultural development, build ecological organic agricultural chain, production safety organic agriculture by product, have very important significance.
Embodiment
Embodiments of the invention 1: the method for the ecological conversion processing industrial sucrose of housefly discarded object comprises the following steps:
(1) fly kind gathers and clean raising domestication: collect the wild housefly in the sucrose place of production, carry out Biology identification, then become fly to raise with the mass ratio milk powder of 1: 1, brown sugar, larva is with the clean cultivation of wheat bran, start nature from larva and go down to posterity more than 5 generations, make it under intensive feeding environment, there is stable reproductive characteristic;
(2) bagasse transforms the induction that adapts to fly strain with stable: bagasse is adopted to mechanical crushing to 400 order, destroy lignin structure, then add wheat bran and water to be mixed with the bagasse composts or fertilisers of cultivating of water content 50%-90%, under environmental temperature 20-30 DEG C, the condition of relative moisture 50%-80%, the common house-fly grub after step (1) domestication is cultivated in induction, the proportioning of inductivity bagasse composts or fertilisers of cultivating is from bagasse powder: wheat bran=1: be increased to gradually bagasse powder: wheat bran=5:5 for 9,2: 8,3: 7,4: 6, every generation is selected in the 3 age in days fly maggot larvas immigration wheat bran that action is active, build is larger and is pupated into fly, until the each polypide quality of survival fly maggot reaches more than 25 milligrams, enter again next proportioning ratio, each proportioning ratio is cultivated 5-10 generation, and each culture process is that the 3 day old larva → fly → milk powder of pupating → sprout wings into is cultivated → separated in the induction of fly blow → bagasse composts or fertilisers of cultivating, brown sugar is raised and laid eggs, again from bagasse powder: wheat bran=5:5 is increased to bagasse powder: wheat bran=6:4, it is active that every generation is selected action, the 4 age in days fly maggot larvas that build is larger move in wheat bran and pupate into fly, in the time that the each polypide quality of survival fly maggot reaches more than 25 milligrams, in this proportioning ratio, continue to cultivate 3-5 generation, make fly strain each side physiological situation reach stable, enter again next proportioning ratio, each proportioning ratio is cultivated 10-15 generation, each culture process is that the 4 day old larva → fly → milk powder of pupating → sprout wings into is cultivated → separated in the induction of fly blow → bagasse composts or fertilisers of cultivating, brown sugar is raised and is laid eggs, its objective is in order to induce housefly to make it adapt to lignin, the nutrient environment etc. in bagasse, make housefly life respectively go through phase biological property and reach nature or wheat bran raising growth standard, obtain thus bagasse and transform the strain of adaptation fly,
(3) seed selection of cellulase high expressed fly strain: the larva that bagasse is transformed to adaptation fly strain inoculation is placed in bagasse powder: wheat bran=6: 4 bagasse composts or fertilisers of cultivating is bred, random packet, in per generation, gets in composts or fertilisers of cultivating crude fiber content and measures minimum group and pupate into fly, adopt the method for genetic screening to obtain the fly strain of secretion cellulase high expressed, after the crude fiber content in composts or fertilisers of cultivating is lower than 15%, raise again 3-5 generation, obtain stable cellulase high expressed fly strain;
(4) induction of cellulase, the two high expressed fly strains of lipase: the high fiber high glucose and high fat fat environment that the larva of cellulase high expressed fly strain is placed in to 60% bagasse, 20% wheat bran, 5% lard, 5% sucrose and 10% albumen auxiliary material (dry maggot powder) composition is bred, process of growth is that fly blow → inoculation bed material → separation 4 day old larva → fly → milk powder of pupating → sprout wings into, brown sugar raising are laid eggs; Circulation 10-15 obtains stable cellulase, the two high expressed fly strains of lipase after generation;
(5) industrial sucrose discarded object fly maggot biological transformation pretreatment: bagasse mechanical crushing to 400 order left and right is 10 by concentration
6the whiterot fungi culture fluid of/ml, water, bagasse powder, then mix with the bagasse powder that do not ferment the bagasse powder after activation than admixture activation 8 hours by the quality of 1: 1: 20 by the mass ratio of 1: 500, the opening 1-3 days that ferments, obtains using modified bagasse powder; 1:0.8-1.2 adds sodium bicarbonate in molasses by weight proportion, and pH value is adjusted to 6.5-7.5, and sulfurous method filter mud is heated to 70-80 DEG C, with isopyknic water flushing, removes cerosin wherein; Then count by weight percentage, get bagasse powder 40%, molasses 15%, filter mud 20%, wheat bran 15%, albumen auxiliary material (dry maggot powder) 10% after modification, and regulate water content to 65%-75%, and be made into fly maggot suitability for industrialized production bed material, make it reach the standard of industrialization fly-maggot feed;
(6) industrialization bagasse transforms the stable of fly strain: the larva of cellulase, the two high expressed fly strains of lipase is placed in to fly maggot suitability for industrialized production bed material and breeds, process of growth is that fly blow → inoculation bed material → separation 4 day old larva → fly → milk powder of pupating → sprout wings into, brown sugar raising are laid eggs; Circulation 10-15 obtains stable industrialization bagasse conversion fly strain after generation (be common house-fly grub growth and breeding in the fly maggot suitability for industrialized production bed material of using modified bagasse powder preparation, reach every 10 kilograms of bed materials and cultivate at least 350 grams of output larvas, each polypide quality more than 25 milligrams), transform fly strain with this and carry out the production of fly maggot industrialization product;
(7) production of fly maggot industrialization product: after the remaining bed material after maggot grow is dried, can make the biological function organic manure for the various qualities of Different Crop through screening allotment; Common house-fly grub with become fly to make animal health-care feed (as being aided with the feedstuff additive product that puparium is made antibacterial effect and had antibiotic substitution effect taking fly-maggot protein as major ingredient) or adopt biotechnology processing to make biomedical raw material or product (as maggot skin, puparium can be used to chitin extraction, fly-maggot protein can be used as the raw material of preparation people with health-care eggs white powder).
Embodiments of the invention 2: the method for the ecological conversion processing industrial sucrose of housefly discarded object comprises the following steps:
(1) fly kind gathers and clean raising domestication: collect the wild housefly in the sucrose place of production, carry out Biology identification, then become fly to raise with the mass ratio milk powder of 1: 1, brown sugar, larva is with the clean cultivation of wheat bran, start nature from larva and go down to posterity more than 5 generations, make it under intensive feeding environment, there is stable reproductive characteristic;
(2) bagasse transforms the induction that adapts to fly strain with stable: adopt mechanical crushing to 200-500 order bagasse, destroy lignin structure, then add wheat bran and water to be mixed with the bagasse composts or fertilisers of cultivating of water content 50%-90%, under environmental temperature 20-30 DEG C, the condition of relative moisture 50%-80%, the common house-fly grub after step (1) domestication is cultivated in induction, the proportioning of inductivity bagasse composts or fertilisers of cultivating is from bagasse powder: wheat bran=1: be increased to gradually bagasse powder: wheat bran=5:5 for 9,2: 8,3: 7,4: 6, every generation is selected in the 3 age in days fly maggot larvas immigration wheat bran that action is active, build is larger and is pupated into fly, until the each polypide quality of survival fly maggot reaches more than 25 milligrams, enter again next proportioning ratio, each proportioning ratio is cultivated 5-10 generation, and each culture process is that the 3 day old larva → fly → milk powder of pupating → sprout wings into is cultivated → separated in the induction of fly blow → bagasse composts or fertilisers of cultivating, brown sugar is raised and laid eggs, again from bagasse powder: wheat bran=5:5 is increased to bagasse powder: wheat bran=6:4, it is active that every generation is selected action, the 4 age in days fly maggot larvas that build is larger move in wheat bran and pupate into fly, in the time that the each polypide quality of survival fly maggot reaches more than 25 milligrams, in this proportioning ratio, continue to cultivate 3-5 generation, make fly strain each side physiological situation reach stable, enter again next proportioning ratio, each proportioning ratio is cultivated 10-15 generation, each culture process is that the 4 day old larva → fly → milk powder of pupating → sprout wings into is cultivated → separated in the induction of fly blow → bagasse composts or fertilisers of cultivating, brown sugar is raised and is laid eggs, make housefly life respectively go through phase biological property and reach nature or wheat bran raising growth standard, obtain thus bagasse and transform the strain of adaptation fly,
(3) seed selection of cellulase high expressed fly strain: the larva that bagasse is transformed to adaptation fly strain inoculation is placed in bagasse powder: wheat bran=6: 4 bagasse composts or fertilisers of cultivating is bred, random packet, in per generation, gets in composts or fertilisers of cultivating crude fiber content and measures minimum group and pupate into fly, adopt the method for genetic screening to obtain the fly strain of secretion cellulase high expressed, after the crude fiber content in composts or fertilisers of cultivating is lower than 15%, raise again 3-5 generation, obtain stable cellulase high expressed fly strain;
(4) induction of cellulase, the two high expressed fly strains of lipase: the high fiber high glucose and high fat fat environment that the larva of cellulase high expressed fly strain is placed in to 60% bagasse, 20% wheat bran, 5% lard, 5% sucrose and 10% albumen auxiliary material (corn flour) composition is bred, process of growth is that fly blow → inoculation bed material → separation 4 day old larva → fly → milk powder of pupating → sprout wings into, brown sugar raising are laid eggs; Circulation 10-15 obtains stable cellulase, the two high expressed fly strains of lipase after generation;
(5) industrial sucrose discarded object fly maggot biological transformation pretreatment: bagasse mechanical crushing, to 200-500 order, is 10 by concentration
8the whiterot fungi culture fluid of/ml, water, bagasse powder, then mix with the bagasse powder that do not ferment the bagasse powder after activation than admixture activation 8 hours by the quality of 1: 1: 20 by the mass ratio of 1: 500, the opening 1-3 days that ferments, obtains using modified bagasse powder; 1:0.8-1.2 adds lime stone in molasses by weight proportion, and pH value is adjusted to 6.5-7.5, and sulfurous method filter mud is heated to 70-80 DEG C, with isopyknic water flushing, removes cerosin wherein; Then count by weight percentage, get bagasse powder 60%, molasses 10%, filter mud 10%, cavings 12%, albumen auxiliary material (corn flour) 8% after modification, and regulate water content to 65%-75%, and be made into fly maggot suitability for industrialized production bed material, make it reach the standard of industrialization fly-maggot feed;
(6) industrialization bagasse transforms the stable of fly strain: the larva of cellulase, the two high expressed fly strains of lipase is placed in to fly maggot suitability for industrialized production bed material and breeds, process of growth is that fly blow → inoculation bed material → separation 4 day old larva → fly → milk powder of pupating → sprout wings into, brown sugar raising are laid eggs; Circulation 10-15 obtains stable industrialization bagasse conversion fly strain after generation (be common house-fly grub growth and breeding in the fly maggot suitability for industrialized production bed material of using modified bagasse powder preparation, reach every 10 kilograms of bed materials and cultivate at least 350 grams of output larvas, each polypide quality more than 25 milligrams), transform fly strain with this and carry out the production of fly maggot industrialization product;
(7) production of fly maggot industrialization product: the remaining bed material after maggot grow is dried and made source (functional) organic fertilizer; Common house-fly grub with become fly to make animal health-care feed or adopt biotechnology processing to make biomedical raw material or product.
Embodiments of the invention 3: the method for the ecological conversion processing industrial sucrose of housefly discarded object comprises the following steps:
(1) fly kind gathers and clean raising domestication: collect the wild housefly in the sucrose place of production, carry out Biology identification, then become fly to raise with the mass ratio milk powder of 1: 1, white sugar, larva is with the clean cultivation of wheat bran, start nature from larva and go down to posterity more than 5 generations, make it under intensive feeding environment, there is stable reproductive characteristic;
(2) bagasse transforms the induction that adapts to fly strain with stable: adopt mechanical crushing to 320-800 order bagasse, destroy lignin structure, then add wheat bran and water to be mixed with the bagasse composts or fertilisers of cultivating of water content 50%-90%, under environmental temperature 20-30 DEG C, the condition of relative moisture 50%-80%, the common house-fly grub after step (1) domestication is cultivated in induction; The proportioning of inductivity bagasse composts or fertilisers of cultivating is from bagasse powder: wheat bran=1: 9 are increased to bagasse powder: wheat bran=5:5 gradually, each proportioning ratio is cultivated 5-10 generation, and each culture process is that the 3 day old larva → fly → milk powder of pupating → sprout wings into is cultivated → separated in the induction of fly blow → bagasse composts or fertilisers of cultivating, white sugar is raised and laid eggs; Again from bagasse powder: wheat bran=5:5 is increased to bagasse powder: wheat bran=6:4, each proportioning ratio is cultivated 10-15 generation, and each culture process is that the 4 day old larva → fly → milk powder of pupating → sprout wings into is cultivated → separated in the induction of fly blow → bagasse composts or fertilisers of cultivating, white sugar is raised and laid eggs; Make housefly life respectively go through phase biological property and reach nature or wheat bran raising growth standard, obtain thus bagasse and transform the strain of adaptation fly;
(3) seed selection of cellulase high expressed fly strain: the larva that bagasse is transformed to adaptation fly strain inoculation is placed in bagasse powder: wheat bran=6: 4 bagasse composts or fertilisers of cultivating is bred, random packet, in per generation, gets in composts or fertilisers of cultivating crude fiber content and measures minimum group and pupate into fly, adopt the method for genetic screening to obtain the fly strain of secretion cellulase high expressed, after the crude fiber content in composts or fertilisers of cultivating is lower than 15%, raise again 3-5 generation, obtain stable cellulase high expressed fly strain;
(4) induction of cellulase, the two high expressed fly strains of lipase: the high fiber high glucose and high fat fat environment that the larva of cellulase high expressed fly strain is placed in to 60% bagasse, 20% wheat bran, 5% lard, 5% sucrose and 10% albumen auxiliary material (colza cake) composition is bred, process of growth is that fly blow → inoculation bed material → separation 4 day old larva → fly → milk powder of pupating → sprout wings into, white sugar raising are laid eggs; Circulation 10-15 obtains stable cellulase, the two high expressed fly strains of lipase after generation;
(5) industrial sucrose discarded object fly maggot biological transformation pretreatment: bagasse mechanical crushing, to 320-800 order, is 10 by concentration
7the whiterot fungi culture fluid of/ml, water, bagasse powder, then mix with the bagasse powder that do not ferment the bagasse powder after activation than admixture activation 8 hours by the quality of 1: 1: 20 by the mass ratio of 1: 500, the opening 1-3 days that ferments, obtains using modified bagasse powder; 1:0.8-1.2 adds sodium bicarbonate in molasses by weight proportion, will
ph value is adjusted to 6.5-7.5, carbonatation process filter mud is heated to 70-80 DEG C, with isopyknic water flushing, removes cerosin wherein, 1:1 adds weakly acidic organic waste water (can be the slurry of cleaning equipment in fly maggot suitability for industrialized production, can be also sanitary wastewater) in mass ratio; Then count by weight percentage, get bagasse powder 50%, molasses 14%, filter mud 17%, wheat bran 10%, albumen auxiliary material (colza cake) 9% after modification, and regulate water content to 65%-75%, and be made into fly maggot suitability for industrialized production bed material, make it reach the standard of industrialization fly-maggot feed;
(6) industrialization bagasse transforms the stable of fly strain: the larva of cellulase, the two high expressed fly strains of lipase is placed in to fly maggot suitability for industrialized production bed material and breeds, process of growth is that fly blow → inoculation bed material → separation 4 day old larva → fly → milk powder of pupating → sprout wings into, white sugar raising are laid eggs; Circulation 10-15 obtains stable industrialization bagasse and transforms fly strain after generation, transform fly strain carry out the production of fly maggot industrialization product with this;
(7) production of fly maggot industrialization product: the remaining bed material after maggot grow is dried and made source (functional) organic fertilizer; Common house-fly grub with become fly to make animal health-care feed or adopt biotechnology processing to make biomedical raw material or product.
In embodiment 1-3, step (1), (2) and (4) described domestication, induction, the wild housefly in locality is carried out to the engineering culture adaptability inducing and acclimating under this specific feeding environment of bagasse, make the common house-fly grub can normal growth when intensive farm in bagasse, reach every 150 grams of fly maggots of 10 kilograms of bagasse composts or fertilisers of cultivating breeding, the each polypide quality of 3 age in days fly maggot more than 25 milligrams, fully transform the organic resource in industrial sucrose discarded object; In addition, for preventing that industrialization bagasse from transforming the degeneration of fly strain, in step (6), transform fly strain introduce stable cellulase, the two high expressed fly strains of lipase every 20 days to industrialization bagasse, the quantity of introducing is each every cage 3%-5%.
Claims (10)
1. the method for the ecological conversion processing industrial sucrose of housefly discarded object, is characterized in that: comprise the following steps:
(1) fly kind gathers and clean raising domestication: collect the wild housefly in the sucrose place of production, become fly to raise with milk powder, sugar, larva cleans cultivation with wheat bran, starts nature go down to posterity more than 5 generations from larva, makes it have stable reproductive characteristic;
(2) bagasse transforms the induction that adapts to fly strain with stable: adopt mechanical crushing to 200-800 order bagasse, destroy lignin structure, then add wheat bran and water to be mixed with the bagasse composts or fertilisers of cultivating of water content 50%-90%, under environmental temperature 20-30 DEG C, the condition of relative moisture 50%-80%, the common house-fly grub after step (1) domestication is cultivated in induction; The proportioning of inductivity bagasse composts or fertilisers of cultivating is from bagasse powder: wheat bran=1: 9 are increased to bagasse powder: wheat bran=5:5 gradually, and each proportioning ratio is cultivated 5-10 generation; Again from bagasse powder: wheat bran=5:5 is increased to bagasse powder: each proportioning ratio of wheat bran=6:4 is cultivated 10-15 generation, make housefly life respectively go through phase biological property and reach nature or wheat bran raising growth standard, obtain thus bagasse and transform the strain of adaptation fly;
(3) seed selection of cellulase high expressed fly strain: the larva that bagasse is transformed to adaptation fly strain inoculation is placed in bagasse powder: wheat bran=6: 4 bagasse composts or fertilisers of cultivating is bred, random packet, in per generation, gets in composts or fertilisers of cultivating crude fiber content and measures minimum group and pupate into fly, adopt the method for genetic screening to obtain the fly strain of secretion cellulase high expressed, after the crude fiber content in composts or fertilisers of cultivating is lower than 15%, raise again 3-5 generation, obtain stable cellulase high expressed fly strain;
(4) induction of cellulase, the two high expressed fly strains of lipase: the high fiber high glucose and high fat fat environment that the larva of cellulase high expressed fly strain is placed in to 60% bagasse, 20% wheat bran, 5% lard, 5% sucrose and 10% albumen auxiliary material composition is bred, circulation 10-15 obtains stable cellulase, the two high expressed fly strains of lipase after generation;
(5) industrial sucrose discarded object fly maggot biological transformation pretreatment: bagasse mechanical crushing, to 200-800 order, is 10 by concentration
6-10
8the whiterot fungi culture fluid of/ml, water, bagasse powder, then mix with the bagasse powder that do not ferment the bagasse powder after activation than admixture activation 8 hours by the quality of 1: 1: 20 by the mass ratio of 1: 500, the opening 1-3 days that ferments, obtains using modified bagasse powder; 1:0.8-1.2 adds pH adjusting agent in molasses by weight proportion, and pH value is adjusted to 6.5-7.5; Filter mud is heated to 70-80 DEG C, with isopyknic water flushing, removes cerosin wherein; Then count by weight percentage, get bagasse powder 40%-60%, molasses 10%-15%, filter mud 10%-20%, wheat bran or cavings 10%-15%, albumen auxiliary material 8%-10% after modification, and regulate water content to 65%-75%, be made into fly maggot suitability for industrialized production bed material, make it reach the standard of industrialization fly-maggot feed;
(6) industrialization bagasse transforms the stable of fly strain: the larva of cellulase, the two high expressed fly strains of lipase is placed in to fly maggot suitability for industrialized production bed material and breeds, circulation 10-15 obtains stable industrialization bagasse and transforms fly strain after generation, transform fly strain carry out the production of fly maggot industrialization product with this;
(7) production of fly maggot industrialization product: the remaining bed material after maggot grow is dried and made source (functional) organic fertilizer; Common house-fly grub with become fly to make animal health-care feed or adopt biotechnology processing to make biomedical raw material or product.
2. the method for the ecological conversion processing industrial sucrose of housefly discarded object according to claim 1, is characterized in that: comprise the following steps:
(1) fly kind gathers and clean raising domestication: collect the wild housefly in the sucrose place of production, carry out Biology identification, then become fly to raise with the mass ratio milk powder of 1: 1, sugar, larva is with the clean cultivation of wheat bran, start nature from larva and go down to posterity more than 5 generations, make it under intensive feeding environment, there is stable reproductive characteristic;
(2) bagasse transforms the induction that adapts to fly strain with stable: bagasse is adopted to mechanical crushing to 400 order, destroy lignin structure, then add wheat bran and water to be mixed with the bagasse composts or fertilisers of cultivating of water content 50%-90%, under environmental temperature 20-30 DEG C, the condition of relative moisture 50%-80%, the common house-fly grub after step (1) domestication is cultivated in induction; The proportioning of inductivity bagasse composts or fertilisers of cultivating is from bagasse powder: wheat bran=1: 9 are increased to bagasse powder: wheat bran=5:5 gradually, each proportioning ratio is cultivated 5-10 generation, and each culture process is that the 3 day old larva → fly → milk powder of pupating → sprout wings into is cultivated → separated in the induction of fly blow → bagasse composts or fertilisers of cultivating, sugar is raised and laid eggs; Again from bagasse powder: wheat bran=5:5 is increased to bagasse powder: wheat bran=6:4, each proportioning ratio is cultivated 10-15 generation, and each culture process is that the 4 day old larva → fly → milk powder of pupating → sprout wings into is cultivated → separated in the induction of fly blow → bagasse composts or fertilisers of cultivating, sugar is raised and laid eggs; Make housefly life respectively go through phase biological property and reach nature or wheat bran raising growth standard, obtain thus bagasse and transform the strain of adaptation fly;
(3) seed selection of cellulase high expressed fly strain: the larva that bagasse is transformed to adaptation fly strain inoculation is placed in bagasse powder: wheat bran=6: 4 bagasse composts or fertilisers of cultivating is bred, random packet, in per generation, gets in composts or fertilisers of cultivating crude fiber content and measures minimum group and pupate into fly, adopt the method for genetic screening to obtain the fly strain of secretion cellulase high expressed, after the crude fiber content in composts or fertilisers of cultivating is lower than 15%, raise again 3-5 generation, obtain stable cellulase high expressed fly strain;
(4) induction of cellulase, the two high expressed fly strains of lipase: the high fiber high glucose and high fat fat environment that the larva of cellulase high expressed fly strain is placed in to 60% bagasse, 20% wheat bran, 5% lard, 5% sucrose and 10% albumen auxiliary material composition is bred, process of growth is that fly blow → inoculation bed material → separation 4 day old larva → fly → milk powder of pupating → sprout wings into, sugar raising are laid eggs; Circulation 10-15 obtains stable cellulase, the two high expressed fly strains of lipase after generation;
(5) industrial sucrose discarded object fly maggot biological transformation pretreatment: bagasse mechanical crushing to 400 order is 10 by concentration
6the whiterot fungi culture fluid of/ml, water, bagasse powder, then mix with the bagasse powder that do not ferment the bagasse powder after activation than admixture activation 8 hours by the quality of 1: 1: 20 by the mass ratio of 1: 500, the opening 1-3 days that ferments, obtains using modified bagasse powder; 1:0.8-1.2 adds pH adjusting agent in molasses by weight proportion, and pH value is adjusted to 6.5-7.5; Filter mud is heated to 70-80 DEG C, with isopyknic water flushing, removes cerosin wherein; Then count by weight percentage, get bagasse powder 40%, molasses 15%, filter mud 20%, wheat bran or cavings 15%, albumen auxiliary material 10% after modification, and regulate water content to 65%-75%, and be made into fly maggot suitability for industrialized production bed material, make it reach the standard of industrialization fly-maggot feed;
(6) industrialization bagasse transforms the stable of fly strain: the larva of cellulase, the two high expressed fly strains of lipase is placed in to fly maggot suitability for industrialized production bed material and breeds, process of growth is that fly blow → inoculation bed material → separation 4 day old larva → fly → milk powder of pupating → sprout wings into, sugar raising are laid eggs; Circulation 10-15 obtains stable industrialization bagasse and transforms fly strain after generation, transform fly strain carry out the production of fly maggot industrialization product with this;
(7) production of fly maggot industrialization product: the remaining bed material after maggot grow is dried and made source (functional) organic fertilizer; Common house-fly grub with become fly to make animal health-care feed or adopt biotechnology processing to make biomedical raw material or product.
3. according to the method for the ecological conversion processing industrial sucrose of housefly described in claim 1 or 2 discarded object, it is characterized in that: in step (6), transform fly strain every 20 days to industrialization bagasse and introduce stable cellulase, the two high expressed fly strains of lipase, the quantity of introducing is each every cage 3%-5%, prevents that industrialization bagasse from transforming the degeneration of fly strain.
4. according to the method for the ecological conversion processing industrial sucrose of housefly described in claim 1 or 2 discarded object, it is characterized in that: the filter mud described in step (5) is sulfurous method filter mud or carbonatation process filter mud.
5. the method for the ecological conversion processing industrial sucrose of housefly discarded object according to claim 4, is characterized in that: carbonatation process filter mud is before preparation fly maggot suitability for industrialized production bed material, and 1:1 adds weakly acidic organic waste water in mass ratio.
6. the method for the ecological conversion processing industrial sucrose of housefly discarded object according to claim 3, is characterized in that: the pH adjusting agent in step (5) is sodium bicarbonate or lime stone.
7. the method for the ecological conversion processing industrial sucrose of housefly discarded object according to claim 3, is characterized in that: step (4), (5) described albumen auxiliary material are dry maggot powder, corn flour, colza cake or dregs of beans.
8. the method for the ecological conversion processing industrial sucrose of housefly discarded object according to claim 3, it is characterized in that: the proportioning of inductivity bagasse composts or fertilisers of cultivating is from bagasse powder in step (2): wheat bran=1: 9 are increased to bagasse powder gradually: wheat bran=5:5 process, every generation is selected in the 3 age in days fly maggot larvas immigration wheat bran that action is active, build is larger and is pupated into fly, until survive, the each polypide quality of fly maggot reaches more than 25 milligrams, then enters next proportioning ratio; Inductivity bagasse composts or fertilisers of cultivating proportioning is from bagasse powder: wheat bran=5:5 is increased to bagasse powder: wheat bran=6:4 process, every generation is selected in the 4 age in days fly maggot larvas immigration wheat bran that action is active, build is larger and is pupated into fly, in the time that the each polypide quality of survival fly maggot reaches more than 25 milligrams, in this proportioning ratio, continue to cultivate 3-5 generation, make fly strain each side physiological situation reach stable, then enter next proportioning ratio.
9. the method for the ecological conversion processing industrial sucrose of housefly discarded object according to claim 3, it is characterized in that: step (1), (2) and (4) described domestication, induction, the wild housefly in locality is carried out to the engineering culture adaptability inducing and acclimating under this specific feeding environment of bagasse, make the common house-fly grub can normal growth when intensive farm in bagasse, reach every 150 grams of fly maggots of 10 kilograms of bagasse composts or fertilisers of cultivating breeding, the each polypide quality of 3 age in days fly maggot more than 25 milligrams, fully transform the organic resource in industrial sucrose discarded object.
10. the method for the ecological conversion processing industrial sucrose of housefly discarded object according to claim 3, it is characterized in that: stable industrialization bagasse described in step (6) transforms fly strain and refers to common house-fly grub growth and breeding in the fly maggot suitability for industrialized production bed material of using modified bagasse powder preparation, reach every 10 kilograms of bed materials and cultivate at least 350 grams of output larvas, each polypide quality more than 25 milligrams.
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| GB1594721A (en) * | 1978-05-03 | 1981-08-05 | Grant D | Maggot breeding |
| CN102783459A (en) * | 2012-07-20 | 2012-11-21 | 贵州博康生物工程有限公司 | Method for ecological conversion of fen-flavor white spirit vinasse musca domestica larva |
| CN102795897A (en) * | 2012-07-20 | 2012-11-28 | 贵州博康生物工程有限公司 | Method for treating soy sauce flavor type white spirit vinasse by propagation and ecological transformation of houseflies |
| CN102807398A (en) * | 2012-07-20 | 2012-12-05 | 贵州博康生物工程有限公司 | Method for recycling spent grains of strong aromatic Chinese spirit by using fly larvae |
| CN102805053A (en) * | 2012-08-21 | 2012-12-05 | 贵州博康生物工程有限公司 | Quick and intensive straw management method based on ecological conversion via housefly metabolism |
| CN102860410A (en) * | 2012-10-12 | 2013-01-09 | 中国水产科学研究院渔业机械仪器研究所 | Method for rapidly producing biological fermentation feed |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113956081A (en) * | 2021-11-10 | 2022-01-21 | 江苏科技大学 | Method for biotransformation of silkworm excrement waste by fly maggot and product application thereof |
| CN113956081B (en) * | 2021-11-10 | 2023-02-28 | 江苏科技大学 | Method for biotransformation of silkworm excrement waste by fly maggot and product application thereof |
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