CN102807398A - Method for recycling spent grains of strong aromatic Chinese spirit by using fly larvae - Google Patents
Method for recycling spent grains of strong aromatic Chinese spirit by using fly larvae Download PDFInfo
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Abstract
The invention discloses a method for recycling spent grains of strong aromatic Chinese spirit by using fly larvae. The method comprises the following steps of: collecting, cleanly breeding and domesticating flies, inducing and stabilizing a fly strain suitable for converting the spent grains of the strong aromatic Chinese spirit, preparing an industrial fly larva protein production bottom material, stabilizing a fly strain for producing the spent grains of the strong aromatic Chinese spirit, and producing a fly larva industrial product. Compared with the prior art, the method has the advantages that seed fly distillers' grains are subjected to ecological conversion, functional induction and screening, passage and engineered breeding by a biotechnology, and are recycled mainly by treating and metabolizing houseflies through a modern engineering technical means, ecological degree and bioavailability are high, the decomposition period is short (about 7 days), the product is diversified and high in quality and added value, near zero emission is realized during production, environmental pollution is avoided, fertilizer efficiency is high through scientific proportioning, the method is incomparable to other methods, and the like.
Description
Technical field
The present invention relates to a kind of method of fly maggot recycling treatment aroma type spirit distiller grain, belong to housefly and culture and the vinasse processing technology field.
Background technology
Liquor already is the important economic class of China, and is in close relations with agricultural; Current production rate has reached 890.8 ten thousand tons/year, mainly is divided into Luzhou-flavor, aromatic type and scent type three major types, and wherein aromatic type accounts for 10%, and Luzhou-flavor accounts for 70%, and scent type accounts for 15%, produces per year to lose to be pickled with grains or in wine more than 30,000,000 tons.Science resource type treating spirit distiller grain how, the development raw material production is a common problem of liquor cleaner production and Sustainable development.
Brewed spirit is a main raw material with food crop such as Chinese sorghum, corn, rice, so a large amount of rice husk (wherein containing more Mierocrystalline cellulose and xylogen) is arranged in the spirit distiller grain, forms a kind of material that is difficult to digest, and is unfavorable for the absorption of animal; Spirit distiller grain acidity is big simultaneously, and moisture content is more than 65%, and biodegradability is strong, and the corruption of very easily going mouldy is if untimely processing can cause great pollution to environment, so must handle vinasse in the wine place of production as early as possible.
On the other hand, containing rich nutrient contents in the vinasse, comprise nutritive substances such as a large amount of residual alcohol, yeast saccharomyces cerevisiae, robust fibre and a spot of plant crude protein, starch, is important renewable organic resource, has great potentiality to be exploited.
The treatment technology of vinasse has much at present, mainly comprises the following aspects:
1, vinasse directly oven dry as feed: owing to contain great number of organic matters and cellulosic in the vinasse, the powder after its oven dry can add in the feed of fowl poultry usefulness as auxiliary material, regulates nutrition; Or direct feed as ruminating animal.The carbonization of the burnt paste of a part organic (fat, protein, starch etc.) causes the product nutritive value to reduce in the vinasse but oven dry can make; Oven dry simultaneously also can make materials such as the organic acid that contains in the vinasse, ester, alcohol, aldehyde volatilize in a large number, and these toxic substances cause topsoil easily.
2, the vinasse microbial fermentation is used as fertilizer: because vinasse itself are exactly the product behind the grain class crop fermented soy liquor, its material properties is fit to the growth of microorganism species very much.What but the liquor manufacturing of China was adopted is the mixing solid phase open fermentation mode of many bacterial classifications; It is the exclusive unique making method of China; This just has very high requirement to the micro-ecological environment in the wine place of production; And adopt the method for microorganism Treating Liquor to lose the poor new external mikrobe dominant strain of often will introducing, and destroy the original microbial balance in wine brewing ground easily, have the risk of the unique liquor region local flavor that influence the open many bacterial classifications mixed solid fermentation formation of China's liquor.And the method fermentation period of microbiological treatment is long, generally needs 30-40 days; Products obtained therefrom is more single, and the product added value of deriving is lower.
3, vinasse are handled back as industrial raw materials: like the aroma type spirit distiller grain method of comprehensive utilization CN101565187B of five-Grain Liquor group employing; To lose after the poor oven dry through screening; The screen overflow rice husk is as behind the fuel; Rice hull ash after the burning is as producing WHITE CARBON BLACK, and screen underflow is as animal-feed, and its weak point also is losing of nutritive ingredient in the drying course; And the WHITE CARBON BLACK industry belongs to high energy consumption, the high pollution industry of country restriction, unavoidably can affect to the environment in the liquor place of production.In 2011 " national industry structural adjustment catalogues " newly-built white carbon black production device is made clear and definite restriction, clearly proposed to eliminate 1.5 ten thousand tons of/year following regular grade white carbon black production devices.Five-Grain Liquor group is that the maximum aromatic Chinese spirit of China is brewageed enterprise, and the husk content in the vinasse is very high, about about 60%; The vinasse in one of which year all can only be produced the WHITE CARBON BLACK about 4000 tons; Though therefore should technology to produce WHITE CARBON BLACK with low cost, quality is better, and certain market prospect is arranged; But, nor meet the policy guidance of the industry restructuring of national energy-saving and emission-reduction owing to the problem of output is not suitable for applying.
Housefly is a cosmopolitan, is the breeding place with organic waste, and it is strong to have reproductivity, can scale clean breed, and polypide is of high nutritive value, and has the characteristics such as ability of administering organic contamination.Bibliographical information is arranged, Musca domestica larva in vinasse to grow density very high.But the inventor's research shows: wine of a great variety since the wine brewing starting material with brewage the different of mode, cause the quality of various vinasse also to differ widely, the natural adaptation property of housefly is difference also.Cultivate Musca domestica larva with spirit distiller grain, because its ethanol content is high, acidity is high, contain objectionable impuritiess such as ETHYLE ACETATE, NSC 8882, cause the fly blow hatching slowly, hatching rate is low, and ripe polypide individuality is little, and is low to the transformation efficiency of spirit distiller grain.Other has the scholar to be reported under the condition of thin up to water cut 85% per 10 kilograms of wine to lose and poorly only can obtain 230 gram Musca domestica larvas, and cultivates fly maggot like this and can produce a large amount of sewage, causes environmental problem.Various vinasse have very big difference because it is brewageed starting material and brewages the different of mode on the composition.Above data proves that all no matter adopt the direct improvement of housefly is that spirit distiller grain or wine are lost and poorly all do not reached efficient resource type treating purpose, and this physico-chemical property with vinasse is relevant.Still do not adopt at present the insect efficient ecological to transform the correlation technique report of spirit distiller grain.
Summary of the invention
The objective of the invention is to: the method that a kind of fly maggot recycling treatment aroma type spirit distiller grain is provided.The present invention carries out pre-treatment to aroma type spirit distiller grain; Housefly is carried out the domestication of aroma type spirit distiller grain biotransformation characteristic; Make Musca domestica larva possess industrialized standard to the ecological conversion capability of spirit distiller grain; Thereby set up Musca domestica larva spirit distiller grain is carried out the bio-transformation production technique, finally reach and lose the poor resource type treating that pollutes; This method can be polluted by the safe and efficient spirit distiller grain of resource type treating on the spot, and products obtained therefrom bio-organic fertilizer, animal health feed and biomedical raw material or product can be applied to aspects such as plant husbandry, livestock industry, fishery and Bio-pharmaceutical Industry.
Technical scheme of the present invention: the method for fly maggot recycling treatment aroma type spirit distiller grain may further comprise the steps:
(1) the fly kind is gathered and cleaning raising domestication: collect local wild housefly, become fly to raise with milk powder, sugar, larva is gone down to posterity more than 5 generations with the breed of wheat bran cleaning naturally, makes it have stable reproductive characteristic;
(2) aroma type spirit distiller grain transforms inducing of adaptation fly strain and stable: with the Musca domestica larva of aroma type spirit distiller grain inducing culture after step (1) domestication, envrionment temperature 20-30 ℃, relative humidity 60%-80%; Inducibility is lost the water cut 50%-80% of poor culture material, and its proportioning is poor from losing: wheat bran=1: 9 increases to gradually loses poor culture material entirely, and each proportioning ratio is cultivated 5-30 generation, obtains aroma type spirit distiller grain thus and transforms and adapt to the fly strain;
(3) the industriallization fly maggot protein is produced the preparation of bed material: with Fe
3+Or Losantin is oxygenant, and per 10 kilograms of aroma type spirit distiller grains add the 10-100g oxygenant, mix, and open the placement 1-2 days adds pH value that alkaline matter regulates aroma type spirit distiller grain again to 6.5-7, obtains aroma type spirit distiller grain modification bed material; Calculate by weight, get aroma type spirit distiller grain modification bed material 60%-80%, wheat bran or cavings or beet pulp 20%-30% and albumen auxiliary material 0-10% and mix, be made into fly maggot protein and produce bed material;
(4) aroma type spirit distiller grain is produced the stable of fly strain: aroma type spirit distiller grain is transformed the larva that adapts to the fly strain place industriallization fly maggot protein production bed material to breed; Circulation 10-15 obtains aroma type spirit distiller grain and produces the fly strain after generation, produce the production that the fly maggot industrialization product is carried out in the fly strain with this;
(5) production of fly maggot industrialization product: bio-organic fertilizer is processed in the remaining bed material oven dry behind the maggot grow; Musca domestica larva with become fly to process the animal health feed or adopt biotechnology to handle and process biomedical raw material or product.
More particularly, preceding method may further comprise the steps:
(1) the fly kind is gathered and cleaning raising domestication: collect local wild housefly; Carry out biological assay, become fly to raise with 1: 1 milk powder of mass ratio, sugar then, larva is cultured with the wheat bran cleaning; Naturally go down to posterity more than 5 generations, make it under intensive feeding environment, have stable reproductive characteristic;
(2) aroma type spirit distiller grain transforms inducing of adaptation fly strain and stable: with the Musca domestica larva of aroma type spirit distiller grain inducing culture after step (1) domestication, envrionment temperature 20-30 ℃, relative humidity 60%-80%; Inducibility is lost the water cut 50%-80% of poor culture material; Its proportioning is poor from losing: wheat bran=1: 9 increase to gradually lose poor: wheat bran=5: 5; Each proportioning ratio is cultivated 5-10 generation, and each process of supporting of being commissioned to train is a fly blow → lose poor inducing culture → separations 3 day old larva → fly → milk powder of pupating → sprout wings into, the sugar raising is laid eggs; Poor from losing again: wheat bran=5: 5 increase to gradually lose poor: wheat bran=7: 3, each proportioning ratio are cultivated 15-20 generation, and each process of supporting of being commissioned to train is a fly blow → lose poor inducing culture → separations 4 day old larva → fly → milk powder of pupating → sprout wings into, the sugar raising is laid eggs; Poor from losing then: wheat bran=8: 2 increases to gradually loses poor culture material entirely, and each proportioning ratio is cultivated 20-30 generation, and each process of supporting of being commissioned to train is a fly blow → lose poor inducing culture → separations 4.5 day old larva → fly → milk powder of pupating → sprout wings into, the sugar raising is laid eggs; Make housefly life respectively go through the phase biological property and reach nature or wheat bran raising growth standard, obtain aroma type spirit distiller grain thus and transform the strain of adaptation fly;
(3) the industriallization fly maggot protein is produced the preparation of bed material: with Fe
3+Or Losantin is oxygenant, and per 10 kilograms of aroma type spirit distiller grains add the 10-100g oxygenant, mix, and open the placement 1-2 days adds pH value that alkaline matter regulates aroma type spirit distiller grain again to 6.5-7, obtains aroma type spirit distiller grain modification bed material; Calculate by weight; Getting aroma type spirit distiller grain modification bed material 70%-79%, wheat bran or cavings or beet pulp 20%-25% and albumen auxiliary material 1%-5% mixes; Be made into fly maggot protein and produce bed material, make its standard that reaches the industriallization fly-maggot feed (protein 12%-15%, glucide 20%-25%, fatty 2%-4%, contain various VITAMINss);
(4) strain of aroma type spirit distiller grain production fly is stable: aroma type spirit distiller grain is transformed the larva that adapts to the fly strain place industriallization fly maggot protein production bed material to breed, process of growth is that fly blow → inoculation bed material → separation 4 day old larva → fly → milk powder of pupating → sprout wings into, sugar raising are laid eggs; Circulation 10-15 obtains stable aroma type spirit distiller grain and produces the fly strain after generation, produce the production that the fly maggot industrialization product is carried out in the fly strain with this;
(5) production of fly maggot industrialization product: bio-organic fertilizer is processed in the remaining bed material oven dry behind the maggot grow; Musca domestica larva with become fly to process the animal health feed or adopt biotechnology to handle and process biomedical raw material or product.
In the preceding method,, and, prevent the degeneration of aroma type spirit distiller grain production fly strain whenever at a distance from the wild housefly of 7 days introducing sterile culture at an amount of Luzhou-flavor vinasse leach liquor of adding in the fly feeding process that becomes of step (2) and step (4).The quantity of introducing wild housefly is preferably each every cage and is less than 10%.
In the preceding method; To lose poor culture material proportioning poor from losing for inducibility in the step (2): wheat bran=1: 9 increase to gradually lose poor: wheat bran=5: 5 process; In each generation, selected in the 3 age in days fly maggot larvas immigration wheat bran that action is active, build is bigger and pupates into fly; Reach more than 25 milligrams up to each polypide quality of survival fly maggot, get into next proportioning ratio again; It is poor from losing that inducibility is lost poor culture material proportioning: wheat bran=5: 5 increase to gradually lose poor: wheat bran=7: 3 process; In each generation, selected in the 4 age in days fly maggot larvas immigration wheat bran that action is active, build is bigger and pupates into fly; When each polypide quality of survival fly maggot reaches more than 25 milligrams; In this proportioning ratio, continue to cultivate 3-5 generation, make fly strain each side physiological situation reach stable, get into next proportioning ratio again; It is poor from losing that inducibility is lost poor culture material proportioning: wheat bran=8: 2 increases to gradually to be lost the poor culture material process entirely; In each generation, selected in the 4.5 age in days fly maggot larvas immigration wheat bran that action is active, build is bigger and pupates into fly; When each polypide quality of survival fly maggot reaches more than 25 milligrams; In this proportioning ratio, continue to cultivate 7-10 generation, make fly strain each side physiological situation reach stable, get into next proportioning ratio again.
In the preceding method; Step (1), (2) described domestication, induce; The through engineering approaches of promptly the wild housefly in locality being carried out under this specific feeding environment of aroma type spirit distiller grain is cultured the flexibility inducing and acclimating; Make the Musca domestica larva can normal growth during intensive farm in spirit distiller grain, reach per 10 kilograms of spirit distiller grains breeding fly maggot, 150 grams, each polypide quality of 3 age in days fly maggots more than 25 milligrams, fully transform the organic resource in the spirit distiller grain.
In the preceding method, the described alkaline matter of step (3) is unslaked lime, sodium hydrogencarbonate or yellow soda ash.
In the preceding method, the described albumen auxiliary material of step (3) is dried maggot powder, Semen Maydis powder, rapeseed cake or dregs of beans etc.
In the preceding method; The said stable aroma type spirit distiller grain of step (4) is produced the industriallization fly maggot protein that the fly strain is meant the aroma type spirit distiller grain preparation of Musca domestica larva after modification and is produced growth and breeding in the bed material, reaches per 10 kilograms of bed materials and cultivates output larva at least 350 grams, each polypide quality more than 25 milligrams.
In the preceding method, step (2) and the used aroma type spirit distiller grain of step (3) are that the aquatic foods after the aromatic Chinese spirit wine brewing are lost poor.
Development along with society; The raising of living standards of the people, the market requirement of liquor is increasing, and large-scale liquor-making enterprise is in order to improve output; On the one hand the demands such as starting material Chinese sorghum of making wine required are increased considerably; Large-scale on the other hand liquor-making enterprise is supported the agricultural development in the places of origin of raw materials in order to obtain the liquor-making raw material of high-quality and safety, begins one after another in its large-scale organic or green proportion of crop planting base of construction, place of production.Be that organic Maotai-flavor liquor of representative and organic aromatic Chinese spirit that Luzhou Old Cellar is representative are brewageed enterprise with Maotai; Be the representative of with all strength building organic raw grain ecologic planting base, five-Grain Liquor has also started at Yibin City in recent years makes organic Chinese sorghum raw material site engineering.The plantation of large-scale organic Chinese sorghum needs a large amount of organic bio-fertilizers, if adopt external fertilizer, can increase the agricultural logistics cost; Produce fertilizer and lose poor microbial fermentation on the spot, have the risk of the local brewed spirit microbial environment of influence.
Because insect has good ecological suitability, no matter how abominable growth conditions is, can carry out self according to environment and regulate, and carries out coevolution, and then form new specific population.In view of housefly is a cosmopolitan, adaptive faculty is strong, can through engineering approaches raises, fast organic waste production efficient organic fertilizer and high-quality fly maggot protein such as converting animal-poultry faeces; And adopt local wild housefly population to induce, adopt mikrobe to administer the risk of spirit distiller grain influence wine brewing ground environment microecological balance on the spot thereby can evade.Thereby the present invention adopts the housefly of cultivating the bio-transformation of adaptation spirit distiller grain to produce the fly strain; Improve transformation efficiency; The resource type treating spirit distiller grain pollutes, and produces the biological organic fertilizer raw-material plantation that is used to make wine, and realizes that the liquor cleaner production and the sustainable ecological organic of agricultural development circulate; The derived product of production high added value simultaneously helps the increment and the expansion of industrial chain.
In the R&D process of technical scheme of the present invention; The contriver has carried out analysis, test to aromatic type, Luzhou-flavor and three kinds of spirit distiller grains of scent type respectively one by one; Because it is brewageed starting material and brewages the different of mode, the composition of three kinds of vinasse also has very big-difference, sees table 1 for details.
The composition contrast of table 1 aroma type spirit distiller grain and other spirit distiller grain
Visible by table 1, though three major types type liquor all is to adopt food crop to brewage to form, owing to selecting materials different with technology, the composition of its vinasse and physico-chemical property also have very big difference.The Luzhou-flavor vinasse are because its raw material types is many, and it is lost poor outstanding feature and is:
1, pH value is lower, and the residual organic acid of making wine is main with acetate+ETHYLE ACETATE and caproic acid+NSC 8882; These two types of materials all have very strong pungency, and especially caproic acid+NSC 8882 is a fragrance matter important in the aromatic Chinese spirit, and NSC 8882 content is very high in the Luzhou-flavor vinasse, are 10 times of aromatic type vinasse, are tens times of scent type vinasse.But caproic acid+NSC 8882 has very strong corrodibility, can the shell and the digestive tube of fly maggot be caused damage, and is unfavorable for the normal growth of fly maggot.
2, also contain the material that other micro-organic acid, organic aldehyde, organosilane ester etc. are unfavorable for maggot grow in the fresh grain stillage.Owing to all adopt high temperature system song in aromatic type and the Luzhou-flavor brewing process,, the growth of fly maggot there is not influence basically so the content of other objectionable impurities such as formaldehyde, methyl alcohol, ethyl n-butyrate etc. is very low in its vinasse.
3, protein in the Luzhou-flavor vinasse, fat, glucide three major types nutrient composition content are lower, and vitamin A, D lack very much in the vinasse in addition, do not reach the feed request of fly maggot suitability for industrialized production, but content of vitamin E are abundanter in the vinasse.
4, the husk class material of remnants is a lot of in the Luzhou-flavor vinasse, is 5-6 times of aromatic type vinasse, because husk can increase the permeability of material, contains a large amount of various VITAMINss in addition in the husk; Therefore the permeability of Luzhou-flavor vinasse is better, helps maggot grow.
In conjunction with the characteristics of Luzhou-flavor vinasse, need handle as follows it:
(1) processing of objectionable impurities in the vinasse: main objectionable impurities is caproic acid+NSC 8882 in the Luzhou-flavor vinasse, and caproic acid can be neutralized through the mode of regulating pH value, and NSC 8882 is a kind of highly stable organosilane ester, and water insoluble; Therefore some stronger oxygenants need in properties-correcting agent, be added in right amount, like Fe
3+Perhaps Losantin resolves into caproic acid and ethanol to impel NSC 8882, reaches the purpose that reduces NSC 8882 content.Other adds alkaline matter (unslaked lime, sodium hydrogencarbonate or yellow soda ash) and will lose poor pH value and be adjusted between the 6.5-7.0 organic acid, organic aldehyde and the organosilane ester material that can neutralize and wherein be unfavorable for maggot grow.
(2) interpolation of nutritive substance in the vinasse: owing to the content of three big nutritive ingredients such as protein in the Luzhou-flavor vinasse is lower; Like 1/2 of the too late aromatic type of crude protein content; Therefore the Luzhou-flavor vinasse need add wheat bran or cavings or beet pulp about 20%-25% when the preparation fly maggot protein is produced bed material; The albumen auxiliary material (like dried maggot powder, Semen Maydis powder, rapeseed cake or dregs of beans etc.) that adds 1%-5% again can reach standard (protein 12%-15%, the glucide 20%-25% of industriallization fly-maggot feed; Fat 2%-4% contains various VITAMINss).
(3) processing of husk in the vinasse:, help maggot grow, thereby need not it is carried out handled because Luzhou-flavor vinasse two-story valley shell content is high.
In addition because the on-site environment of wine brewing is different, in order to keep the micro-ecological environment in liquor country of origin, should adopt the locality wild breed housefly population preferably; Because the on-site weather of wine brewing is different, the controlling factor (temperature, humidity etc.) that the domestication of the wild housefly population in locality is adopted is also different, could under only condition, tame out the best fly strain of industriallization situation like this.Yibin Prefecture, the Sichuan weather at Luzhou-flavor vinasse (is example with the five-Grain Liquor) place is the moistening monsoon climate in subtropics; Be in edge, the Sichuan Basin, mean sea level is lower, 17.5 ℃ of average temperatures of the whole year; Thereby the optimal temperature of local wild housefly breeding is at 25 ℃, and high humidity is about 70%.
The invention provides a kind of biotechnology that can carry out the resource utilization ecological management safely and efficiently to aroma type spirit distiller grain on the spot; Under the situation that does not influence liquor grown place micro-ecological environment; Is new and effective biological organic fertilizer, animal health feed and biomedical raw material or product with a large amount of spirit distiller grains through the housefly bio-transformation; Turn waste into wealth; Meet the proposal that Food and Argriculture OrganizationFAO encouraged exploitation edible insect resources in 2008, realized industrial or agricultural resource circulation and sustainable high value added utilization.
Research shows, the bioactive functions composition in the biological organic fertilizer that the present invention produced in the residual metabolite of fly maggot can reduce and decompose fertilizer and pesticide in soil residue and play the heavy metals immobilization effect; The fly maggot protein of being produced has the function of alternative livestock and poultry Antibiotic Additive, can support safe animal products production.Thereby the present invention helps forming agriculture efficiently organic production closed cycle, is the current new and high technology of utilizing, and carries out the optimal path of ecological organic agricultural construction; Be particularly suitable for the production of the higher high-end agricultural prods of quality requirements such as organic agronomic crop plantation, thereby form high-quality organic liquor production benign cycle of " organic grow-brewed spirit-vinasse housefly transforms and produces fertilizer-organic grow ".
Compared with prior art, the present invention adopt biotechnology carry out the ecological transformation function of kind of fly vinasse induce and screen, go down to posterity, through engineering approaches raises, and adopts the modernization project technique means; It is master's vinasse resource type treating that realization is handled metabolism with housefly, and it is high to have the ecology degree, and bioavailability is big; Decomposed period short (about 7 days), product diversification, quality height, resource high added valueization; The production near zero release, non-environmental-pollution, the unrivaled advantage of high other method of scientific matching fertilizer efficiency; The novel biological fertilizer that the present invention produced has the biological pesticide effect, and fly maggot protein has the animal of enhancing resistibility, reduces the effect that microbiotic uses, and is the green agricultural material product that China encourages industrialization; And the used auxiliary material of the present invention, housefly can protect the micro-ecological environment in the wine place of production to greatest extent all from the locality, guarantees the country of origin local flavor and the genuine characteristic of China's liquor.
Embodiment
Embodiments of the invention 1: the method for fly maggot recycling treatment Luzhou-flavor (like five-Grain Liquor) spirit distiller grain may further comprise the steps:
(1) the fly kind is gathered and cleaning raising domestication: collect local wild housefly, grow moral " Chinese common fly class key " according to model and carry out biological assay, place 80cm * 30cm * 30cm Stainless Steel Wire cleaning insect rearging cage; Become fly to raise with 1: 1 milk powder of mass ratio, brown sugar; Larva is cultured 20 ℃-30 ℃ of room temps, relative humidity 60%-80% with the wheat bran cleaning; The illumination rhythm and pace of moving things is as the criterion with local environment; Collect fly blow every day and be inoculated in moisture 65% left and right sides wheat bran and raise, 3 instar larvaes of collecting body weight >=20mg on the 5th are pupated in moisture 15% wheat bran, select ellipse to be about the 6.0mm pupa then and place the cleaning rearging cage to sprout wings into fly; Lay eggs with milk powder, brown sugar raising, get into s-generation cleaning and raise; Naturally go down to posterity and protect after 5 generations kind, make it under intensive feeding environment, have stable reproductive characteristic.
(2) aroma type spirit distiller grain transforms inducing of adaptation fly strain and stable: lose the Musca domestica larva of poor inducing culture after step (1) domestication, envrionment temperature 20-30 ℃, relative humidity 60%-80% with the aquatic foods after the aromatic Chinese spirit wine brewing; Inducibility is lost the water cut 50%-80% of poor culture material; Its proportioning is poor from losing: wheat bran=1: 9, increased to gradually in 2: 8,3: 7,4: 6 lose poor: wheat bran=5: 5; In each generation, selected in the 3 age in days fly maggot larvas immigration wheat bran that action is active, build is bigger and pupates into fly; Reach more than 25 milligrams up to each polypide quality of survival fly maggot, get into next proportioning ratio again; Each proportioning ratio is cultivated 5-10 generation, and each process of supporting of being commissioned to train is a fly blow → lose poor inducing culture → separations 3 day old larva → fly → milk powder of pupating → sprout wings into, the brown sugar raising is laid eggs; Because objectionable impurities mainly is caproic acid+NSC 8882 in the Luzhou-flavor vinasse, it has stronger corrodibility and pungency, lose poor: maggot grow began obviously to slow down even have the phenomena of mortality after wheat bran surpassed 5: 5.Poor from losing again: wheat bran=5: 5, gradually increase at 6: 4 lose poor: wheat bran=7: 3; In each generation, selected in the 4 age in days fly maggot larvas immigration wheat bran that action is active, build is bigger and pupates into fly; When each polypide quality of survival fly maggot reaches more than 25 milligrams; In this proportioning ratio, continue to cultivate 3-5 generation, make fly strain each side physiological situation reach stable, get into next proportioning ratio again; Each proportioning ratio is cultivated 15-20 generation, and each process of supporting of being commissioned to train is a fly blow → lose poor inducing culture → separations 4 day old larva → fly → milk powder of pupating → sprout wings into, the brown sugar raising is laid eggs; Because nutritive ingredient (protein, fat, glucide) content is less in the Luzhou-flavor vinasse, lose poor: maggot grow was not slowly even pupated more after wheat bran surpassed 7: 3.Poor from losing then: as wheat bran=8: 2, gradually to increase at 9: 1 and lose poor culture material (being 10:0) entirely; In each generation, selected in the 4.5 age in days fly maggot larvas immigration wheat bran that action is active, build is bigger and pupates into fly; When each polypide quality of survival fly maggot reaches more than 25 milligrams; In this proportioning ratio, continue to cultivate 7-10 generation, make fly strain each side physiological situation reach stable, get into next proportioning ratio again; Each proportioning ratio is cultivated 20-30 generation, and each process of supporting of being commissioned to train is a fly blow → lose poor inducing culture → separations 4.5 day old larva → fly → milk powder of pupating → sprout wings into, the brown sugar raising is laid eggs; Its objective is in order to induce housefly to make its objectionable impurities that adapts to aroma type spirit distiller grain, nutrient environment etc.; Make through engineering approaches raising housefly life respectively go through the phase biological property and reach nature or wheat bran raising growth standard, obtain aroma type spirit distiller grain thus and transform the strain of adaptation fly.
(3) the industriallization fly maggot protein is produced the preparation of bed material: with Fe
3+Be oxygenant, per 10 kilograms of aroma type spirit distiller grains add the 10-100g oxygenant, mix, and open the placement 1-2 days adds unslaked lime again and regulate the aromatic Chinese spirit aquatic foods and lose poor pH value and reach 6.5-7, obtains aroma type spirit distiller grain modification bed material; Calculate by weight; Getting aroma type spirit distiller grain modification bed material 73%, wheat bran 25% and albumen auxiliary material (dried maggot powder) 2% mixes; Be made into fly maggot protein and produce bed material, make its standard that reaches the industriallization fly-maggot feed (protein 12%-15%, glucide 20%-25%, fatty 2%-4%, contain various VITAMINss).Because main objectionable impurities is caproic acid+NSC 8882 in the aroma type spirit distiller grain, caproic acid can be neutralized through the mode of regulating pH value, and NSC 8882 is a kind of highly stable organosilane ester, and water insoluble; Therefore some stronger oxygenants need in properties-correcting agent, be added in right amount, like Fe
3+Perhaps Losantin resolves into caproic acid and ethanol to impel NSC 8882, reaches the purpose that reduces NSC 8882 content.
(4) strain of aroma type spirit distiller grain production fly is stable: aroma type spirit distiller grain is transformed the larva that adapts to the fly strain place industriallization fly maggot protein production bed material to breed, process of growth is that fly blow → inoculation bed material → separation 4 day old larva → fly → milk powder of pupating → sprout wings into, brown sugar raising are laid eggs; The circulation 10-15 aroma type spirit distiller grain that acquisition can intensively be raised, growth and breeding is stable after generation is produced the fly strain, and (the industriallization fly maggot protein that is the aroma type spirit distiller grain preparation of Musca domestica larva after modification is produced growth and breeding in the bed material; Reach per 10 kilograms of bed materials and cultivate output larva at least 450 grams, each polypide quality more than 25 milligrams), produce the production that the fly maggot industrialization product is carried out in the fly strain with this.
(5) production of fly maggot industrialization product: after the remaining bed material oven dry behind the maggot grow; Can process biological function organic fertilizer through the screening allotment to the various qualities of Different Crop; Musca domestica larva with become fly to process animal health feed (as being that major ingredient is aided with the feedstuff additive product that puparium is processed antibacterial effect and the microbiotic substitution effect is arranged with the fly maggot protein) or adopt biotechnology to handle and process biomedical raw material or product (can be used to chitin extraction like maggot skin, puparium, fly maggot protein can be used as the raw material of preparation human health care protein powder).
Produce the degeneration of fly strain in order to prevent aroma type spirit distiller grain; An amount of Luzhou-flavor vinasse leach liquor of adding in the fly feeding process that becomes in abovementioned steps (2) and step (4); And whenever at a distance from the wild housefly of 7 days introducing sterile culture, the quantity of introducing wild housefly is that each every cage is less than 10%.
Abovementioned steps (1), (2) described domestication, induce, the through engineering approaches of promptly the wild housefly in locality being carried out under this specific feeding environment of aroma type spirit distiller grain is cultured the flexibility inducing and acclimating, makes the Musca domestica larva can normal growth during intensive farm in spirit distiller grain; Reach per 10 kilograms of spirit distiller grains breeding fly maggot, 150 grams, each polypide quality of 3 age in days fly maggots more than 25 milligrams, be 6-7 days to pupating, sprouted wings in 2-3 days by egg development; Eclosion rate>50%, the 2-3 days female flies in back that sprout wings begin to lay eggs, and egg-laying time continues about 10-15 days; Become fly generally to survive 30-40 days; The hatching rate of fly blow>50%, can fully transform the organic resource in the spirit distiller grain, administer and pollute.
Embodiments of the invention 2: the method for fly maggot recycling treatment aroma type spirit distiller grain may further comprise the steps:
(1) the fly kind is gathered and cleaning raising domestication: collect local wild housefly; Grow moral " Chinese common fly class key " according to model and carry out biological assay; According to environment (about 25 ℃ of temperature, humidity about 70%, the illumination etc.) condition of local housefly natural propagation, become fly to raise with 1: 1 milk powder of mass ratio, brown sugar then, larva is cultured with the wheat bran cleaning; Naturally go down to posterity more than 5 generations, make it under intensive feeding environment, have stable reproductive characteristic.
(2) aroma type spirit distiller grain transforms inducing of adaptation fly strain and stable: with the Musca domestica larva of aroma type spirit distiller grain inducing culture after step (1) domestication, envrionment temperature 20-30 ℃, relative humidity 60%-80%; Inducibility is lost the water cut 50%-80% of poor culture material; Its proportioning is poor from losing: wheat bran=1: 9, increased to gradually in 2: 8,3: 7,4: 6 lose poor: wheat bran=5: 5; In each generation, selected in the 3 age in days fly maggot larvas immigration wheat bran that action is active, build is bigger and pupates into fly; Reach more than 25 milligrams up to each polypide quality of survival fly maggot, get into next proportioning ratio again; Each proportioning ratio is cultivated 5-10 generation, and each process of supporting of being commissioned to train is a fly blow → lose poor inducing culture → separations 3 day old larva → fly → milk powder of pupating → sprout wings into, the brown sugar raising is laid eggs; Poor from losing again: wheat bran=5: 5, gradually increase at 6: 4 lose poor: wheat bran=7: 3; In each generation, selected in the 4 age in days fly maggot larvas immigration wheat bran that action is active, build is bigger and pupates into fly; When each polypide quality of survival fly maggot reaches more than 25 milligrams; In this proportioning ratio, continue to cultivate 3-5 generation, make fly strain each side physiological situation reach stable, get into next proportioning ratio again; Each proportioning ratio is cultivated 15-20 generation, and each process of supporting of being commissioned to train is a fly blow → lose poor inducing culture → separations 4 day old larva → fly → milk powder of pupating → sprout wings into, the brown sugar raising is laid eggs; Poor from losing then: as wheat bran=8: 2, gradually to increase at 9: 1 and lose poor culture material (being 10:0) entirely; In each generation, selected in the 4.5 age in days fly maggot larvas immigration wheat bran that action is active, build is bigger and pupates into fly; When each polypide quality of survival fly maggot reaches more than 25 milligrams; In this proportioning ratio, continue to cultivate 7-10 generation, make fly strain each side physiological situation reach stable, get into next proportioning ratio again; Each proportioning ratio is cultivated 20-30 generation, and each process of supporting of being commissioned to train is a fly blow → lose poor inducing culture → separations 4.5 day old larva → fly → milk powder of pupating → sprout wings into, the brown sugar raising is laid eggs; Its objective is in order to induce housefly to make its objectionable impurities that adapts to aroma type spirit distiller grain, nutrient environment etc.; Make through engineering approaches raising housefly life respectively go through the phase biological property and reach nature or wheat bran raising growth standard, obtain aroma type spirit distiller grain thus and transform the strain of adaptation fly.
(3) the industriallization fly maggot protein is produced the preparation of bed material: with the Losantin is oxygenant; Per 10 kilograms of aroma type spirit distiller grains add the 10-100g oxygenant; Mix; The open placement 1-2 days, the pH value that adds sodium hydrogencarbonate adjusting aroma type spirit distiller grain again obtains aroma type spirit distiller grain modification bed material to 6.5-7; Calculate by weight; Getting aroma type spirit distiller grain modification bed material 75%, cavings 20% and albumen auxiliary material (Semen Maydis powder) 5% mixes; Be made into fly maggot protein and produce bed material, make its standard that reaches the industriallization fly-maggot feed (protein 12%-15%, glucide 20%-25%, fatty 2%-4%, contain various VITAMINss).
(4) strain of aroma type spirit distiller grain production fly is stable: aroma type spirit distiller grain is transformed the larva that adapts to the fly strain place industriallization fly maggot protein production bed material to breed, process of growth is that fly blow → inoculation bed material → separation 4 day old larva → fly → milk powder of pupating → sprout wings into, brown sugar raising are laid eggs; The circulation 10-15 aroma type spirit distiller grain that acquisition can intensively be raised, growth and breeding is stable after generation is produced the fly strain, and (the industriallization fly maggot protein that is the aroma type spirit distiller grain preparation of Musca domestica larva after modification is produced growth and breeding in the bed material; Reach per 10 kilograms of bed materials and cultivate output larva at least 400 grams, each polypide quality more than 25 milligrams), produce the production that the fly maggot industrialization product is carried out in the fly strain with this.
(5) production of fly maggot industrialization product: bio-organic fertilizer is processed in the remaining bed material oven dry behind the maggot grow; Musca domestica larva with become fly to process animal health feed (as being that major ingredient is aided with the feedstuff additive product that puparium is processed antibacterial effect and the microbiotic substitution effect is arranged with the fly maggot protein) or adopt biotechnology to handle and process biomedical raw material or product (can be used to chitin extraction like maggot skin, puparium, fly maggot protein can be used as the raw material of preparation human health care protein powder).
Produce the degeneration of fly strain in order to prevent aroma type spirit distiller grain; An amount of Luzhou-flavor vinasse leach liquor of adding in the fly feeding process that becomes in abovementioned steps (2) and step (4); And whenever at a distance from the wild housefly of 7 days introducing sterile culture, the quantity of introducing wild housefly is that each every cage is less than 10%.
Abovementioned steps (1), (2) described domestication, induce; The through engineering approaches of promptly the wild housefly in locality being carried out under this specific feeding environment of aroma type spirit distiller grain is cultured the flexibility inducing and acclimating; Make the Musca domestica larva can normal growth during intensive farm in spirit distiller grain; Reach per 10 kilograms of spirit distiller grains breeding fly maggot, 150 grams, each polypide quality of 3 age in days fly maggots more than 25 milligrams, fully transform the organic resource in the spirit distiller grain.
Embodiments of the invention 3: the method for fly maggot recycling treatment aroma type spirit distiller grain may further comprise the steps:
(1) the fly kind is gathered and cleaning raising domestication: collect local wild housefly; Carry out biological assay, become fly to raise with 1: 1 milk powder of mass ratio, white sugar then, larva is cultured with the wheat bran cleaning; Naturally go down to posterity more than 5 generations, make it under intensive feeding environment, have stable reproductive characteristic.
(2) aroma type spirit distiller grain transforms inducing of adaptation fly strain and stable: with the Musca domestica larva of aroma type spirit distiller grain inducing culture after step (1) domestication, envrionment temperature 20-30 ℃, relative humidity 60%-80%; Inducibility is lost the water cut 50%-80% of poor culture material; Its proportioning is poor from losing: wheat bran=1: 9 increase to gradually lose poor: wheat bran=5: 5; In each generation, selected in the 3 age in days fly maggot larvas immigration wheat bran that action is active, build is bigger and pupates into fly; Reach more than 25 milligrams up to each polypide quality of survival fly maggot, get into next proportioning ratio again; Each proportioning ratio is cultivated 5-10 generation, and each process of supporting of being commissioned to train is a fly blow → lose poor inducing culture → separations 3 day old larva → fly → milk powder of pupating → sprout wings into, the white sugar raising is laid eggs; Poor from losing again: wheat bran=5: 5 increase to gradually lose poor: wheat bran=7: 3; In each generation, selected in the 4 age in days fly maggot larvas immigration wheat bran that action is active, build is bigger and pupates into fly; When each polypide quality of survival fly maggot reaches more than 25 milligrams; In this proportioning ratio, continue to cultivate 3-5 generation, make fly strain each side physiological situation reach stable, get into next proportioning ratio again; Each proportioning ratio is cultivated 15-20 generation, and each process of supporting of being commissioned to train is a fly blow → lose poor inducing culture → separations 4 day old larva → fly → milk powder of pupating → sprout wings into, the white sugar raising is laid eggs; Poor from losing then: wheat bran=8: 2 increases to gradually loses poor culture material entirely; In each generation, selected in the 4.5 age in days fly maggot larvas immigration wheat bran that action is active, build is bigger and pupates into fly; When each polypide quality of survival fly maggot reaches more than 25 milligrams; In this proportioning ratio, continue to cultivate 7-10 generation, make fly strain each side physiological situation reach stable, get into next proportioning ratio again; Each proportioning ratio is cultivated 20-30 generation, and each process of supporting of being commissioned to train is a fly blow → lose poor inducing culture → separations 4.5 day old larva → fly → milk powder of pupating → sprout wings into, the white sugar raising is laid eggs; Make housefly life respectively go through the phase biological property and reach nature or wheat bran raising growth standard, obtain aroma type spirit distiller grain thus and transform the strain of adaptation fly.
(3) the industriallization fly maggot protein is produced the preparation of bed material: with Fe
3+Be oxygenant, per 10 kilograms of aroma type spirit distiller grains add the 10-100g oxygenant, mix, the open placement 1-2 days, and the pH value that adds yellow soda ash adjusting aroma type spirit distiller grain again obtains aroma type spirit distiller grain modification bed material to 6.5-7; Calculate by weight, get aroma type spirit distiller grain modification bed material 79%, beet pulp 20% and albumen auxiliary material (rapeseed cake or dregs of beans) 1% and mix, be made into fly maggot protein and produce bed material, make it reach the standard of industriallization fly-maggot feed.
(4) strain of aroma type spirit distiller grain production fly is stable: aroma type spirit distiller grain is transformed the larva that adapts to the fly strain place industriallization fly maggot protein production bed material to breed, process of growth is that fly blow → inoculation bed material → separation 4 day old larva → fly → milk powder of pupating → sprout wings into, white sugar raising are laid eggs; Circulation 10-15 obtains stable aroma type spirit distiller grain and produces fly strain (growth and breeding in the industriallization fly maggot protein production bed material of the aroma type spirit distiller grain preparation of Musca domestica larva after modification after generation; Reach per 10 kilograms of bed materials and cultivate output larva at least 350 grams, each polypide quality more than 25 milligrams), produce the production that the fly maggot industrialization product is carried out in the fly strain with this.
(5) production of fly maggot industrialization product: bio-organic fertilizer is processed in the remaining bed material oven dry behind the maggot grow; Musca domestica larva with become fly to process the animal health feed or adopt biotechnology to handle and process biomedical raw material or product.
Produce the degeneration of fly strain in order to prevent aroma type spirit distiller grain; At an amount of Luzhou-flavor vinasse leach liquor of adding in the fly feeding process that becomes of abovementioned steps (2) and step (4), and every wild housefly (quantity is that each every cage is less than 10%) of introducing sterile culture at a distance from 7 days.
Claims (10)
1. the method for fly maggot recycling treatment aroma type spirit distiller grain is characterized in that: may further comprise the steps:
(1) the fly kind is gathered and cleaning raising domestication: collect local wild housefly, become fly to raise with milk powder, sugar, larva is gone down to posterity more than 5 generations with the breed of wheat bran cleaning naturally, makes it have stable reproductive characteristic;
(2) aroma type spirit distiller grain transforms inducing of adaptation fly strain and stable: with the Musca domestica larva of aroma type spirit distiller grain inducing culture after step (1) domestication, envrionment temperature 20-30 ℃, relative humidity 60%-80%; Inducibility is lost the water cut 50%-80% of poor culture material, and its proportioning is poor from losing: wheat bran=1: 9 increases to gradually loses poor culture material entirely, and each proportioning ratio is cultivated 5-30 generation, obtains aroma type spirit distiller grain thus and transforms and adapt to the fly strain;
(3) the industriallization fly maggot protein is produced the preparation of bed material: with Fe
3+Or Losantin is oxygenant, and per 10 kilograms of aroma type spirit distiller grains add the 10-100g oxygenant, mix, and open the placement 1-2 days adds pH value that alkaline matter regulates aroma type spirit distiller grain again to 6.5-7, obtains aroma type spirit distiller grain modification bed material; Calculate by weight, get aroma type spirit distiller grain modification bed material 60%-80%, wheat bran or cavings or beet pulp 20%-30% and albumen auxiliary material 0-10% and mix, be made into fly maggot protein and produce bed material;
(4) aroma type spirit distiller grain is produced the stable of fly strain: aroma type spirit distiller grain is transformed the larva that adapts to the fly strain place industriallization fly maggot protein production bed material to breed; Circulation 10-15 obtains aroma type spirit distiller grain and produces the fly strain after generation, produce the production that the fly maggot industrialization product is carried out in the fly strain with this;
(5) production of fly maggot industrialization product: bio-organic fertilizer is processed in the remaining bed material oven dry behind the maggot grow; Musca domestica larva with become fly to process the animal health feed or adopt biotechnology to handle and process biomedical raw material or product.
2. according to the method for the said fly maggot recycling treatment of claim 1 aroma type spirit distiller grain, it is characterized in that: may further comprise the steps:
(1) the fly kind is gathered and cleaning raising domestication: collect local wild housefly; Carry out biological assay, become fly to raise with 1: 1 milk powder of mass ratio, sugar then, larva is cultured with the wheat bran cleaning; Naturally go down to posterity more than 5 generations, make it under intensive feeding environment, have stable reproductive characteristic;
(2) aroma type spirit distiller grain transforms inducing of adaptation fly strain and stable: with the Musca domestica larva of aroma type spirit distiller grain inducing culture after step (1) domestication, envrionment temperature 20-30 ℃, relative humidity 60%-80%; Inducibility is lost the water cut 50%-80% of poor culture material; Its proportioning is poor from losing: wheat bran=1: 9 increase to gradually lose poor: wheat bran=5: 5; Each proportioning ratio is cultivated 5-10 generation, and each process of supporting of being commissioned to train is a fly blow → lose poor inducing culture → separations 3 day old larva → fly → milk powder of pupating → sprout wings into, the sugar raising is laid eggs; Poor from losing again: wheat bran=5: 5 increase to gradually lose poor: wheat bran=7: 3, each proportioning ratio are cultivated 15-20 generation, and each process of supporting of being commissioned to train is a fly blow → lose poor inducing culture → separations 4 day old larva → fly → milk powder of pupating → sprout wings into, the sugar raising is laid eggs; Poor from losing then: wheat bran=8: 2 increases to gradually loses poor culture material entirely, and each proportioning ratio is cultivated 20-30 generation, and each process of supporting of being commissioned to train is a fly blow → lose poor inducing culture → separations 4.5 day old larva → fly → milk powder of pupating → sprout wings into, the sugar raising is laid eggs; Make housefly life respectively go through the phase biological property and reach nature or wheat bran raising growth standard, obtain aroma type spirit distiller grain thus and transform the strain of adaptation fly;
(3) the industriallization fly maggot protein is produced the preparation of bed material: with Fe
3+Or Losantin is oxygenant, and per 10 kilograms of aroma type spirit distiller grains add the 10-100g oxygenant, mix, and open the placement 1-2 days adds pH value that alkaline matter regulates aroma type spirit distiller grain again to 6.5-7, obtains aroma type spirit distiller grain modification bed material; Calculate by weight, get aroma type spirit distiller grain modification bed material 70%-79%, wheat bran or cavings or beet pulp 20%-25% and albumen auxiliary material 1%-5% and mix, be made into fly maggot protein and produce bed material, make it reach the standard of industriallization fly-maggot feed;
(4) strain of aroma type spirit distiller grain production fly is stable: aroma type spirit distiller grain is transformed the larva that adapts to the fly strain place industriallization fly maggot protein production bed material to breed, process of growth is that fly blow → inoculation bed material → separation 4 day old larva → fly → milk powder of pupating → sprout wings into, sugar raising are laid eggs; Circulation 10-15 obtains stable aroma type spirit distiller grain and produces the fly strain after generation, produce the production that the fly maggot industrialization product is carried out in the fly strain with this;
(5) production of fly maggot industrialization product: bio-organic fertilizer is processed in the remaining bed material oven dry behind the maggot grow; Musca domestica larva with become fly to process the animal health feed or adopt biotechnology to handle and process biomedical raw material or product.
3. according to the method for claim 1 or 2 said fly maggot recycling treatment aroma type spirit distiller grains; It is characterized in that: at an amount of Luzhou-flavor vinasse leach liquor of adding in the fly feeding process that becomes of step (2) and step (4); And, prevent the degeneration of aroma type spirit distiller grain production fly strain whenever at a distance from the wild housefly of 7 days introducing sterile culture.
4. according to the method for the said fly maggot recycling treatment of claim 3 aroma type spirit distiller grain, it is characterized in that: the quantity of introducing wild housefly is that each every cage is less than 10%.
5. according to the method for claim 1 or 2 said fly maggot recycling treatment aroma type spirit distiller grains; It is characterized in that: to lose poor culture material proportioning poor from losing for inducibility in the step (2): wheat bran=1: 9 increase to gradually lose poor: wheat bran=5: 5 process; In each generation, selected in the 3 age in days fly maggot larvas immigration wheat bran that action is active, build is bigger and pupates into fly; Reach more than 25 milligrams up to each polypide quality of survival fly maggot, get into next proportioning ratio again; It is poor from losing that inducibility is lost poor culture material proportioning: wheat bran=5: 5 increase to gradually lose poor: wheat bran=7: 3 process; In each generation, selected in the 4 age in days fly maggot larvas immigration wheat bran that action is active, build is bigger and pupates into fly; When each polypide quality of survival fly maggot reaches more than 25 milligrams; In this proportioning ratio, continue to cultivate 3-5 generation, make fly strain each side physiological situation reach stable, get into next proportioning ratio again; It is poor from losing that inducibility is lost poor culture material proportioning: wheat bran=8: 2 increases to gradually to be lost the poor culture material process entirely; In each generation, selected in the 4.5 age in days fly maggot larvas immigration wheat bran that action is active, build is bigger and pupates into fly; When each polypide quality of survival fly maggot reaches more than 25 milligrams; In this proportioning ratio, continue to cultivate 7-10 generation, make fly strain each side physiological situation reach stable, get into next proportioning ratio again.
6. according to the method for claim 1 or 2 said fly maggot recycling treatment aroma type spirit distiller grains; It is characterized in that: step (1), (2) described domestication, induce; The through engineering approaches of promptly the wild housefly in locality being carried out under this specific feeding environment of aroma type spirit distiller grain is cultured the flexibility inducing and acclimating; Make the Musca domestica larva can normal growth during intensive farm in spirit distiller grain; Reach per 10 kilograms of spirit distiller grains breeding fly maggot, 150 grams, each polypide quality of 3 age in days fly maggots more than 25 milligrams, fully transform the organic resource in the spirit distiller grain.
7. according to the method for claim 1 or 2 said fly maggot recycling treatment aroma type spirit distiller grains, it is characterized in that: the described alkaline matter of step (3) is unslaked lime, sodium hydrogencarbonate or yellow soda ash.
8. according to the method for claim 1 or 2 said fly maggot recycling treatment aroma type spirit distiller grains, it is characterized in that: the described albumen auxiliary material of step (3) is dried maggot powder, Semen Maydis powder, rapeseed cake or dregs of beans.
9. according to the method for the said fly maggot recycling treatment of claim 2 aroma type spirit distiller grain; It is characterized in that: stable aroma type spirit distiller grain described in the step (4) is produced the industriallization fly maggot protein that the fly strain is meant the aroma type spirit distiller grain preparation of Musca domestica larva after modification and is produced growth and breeding in the bed material, reaches per 10 kilograms of bed materials and cultivates output larva at least 350 grams, each polypide quality more than 25 milligrams.
10. according to the method for claim 1 or 2 fly maggot recycling treatment aroma type spirit distiller grains, it is characterized in that: step (2) and the used aroma type spirit distiller grain of step (3) are that the aquatic foods after the aromatic Chinese spirit wine brewing are lost poor.
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| CN108552119A (en) * | 2018-04-12 | 2018-09-21 | 贵州医科大学 | Polygonum capitatum discards the processing method of the dregs of a decoction |
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| CN102807398B (en) | 2014-01-29 |
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