CN109619045A - A method of bioconversion being carried out to gibberellic acid bacteria residue using black soldier flies larva - Google Patents
A method of bioconversion being carried out to gibberellic acid bacteria residue using black soldier flies larva Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
Abstract
The invention discloses a kind of methods for carrying out bioconversion to gibberellic acid bacteria residue using black soldier flies larva, steps are as follows: the preparation of black soldier flies egg hatching material: first pre-processing to gibberellic acid bacteria residue, pretreated gibberellic acid bacteria residue is mixed with auxiliary material again, adjusts water content；The access of black soldier flies ovum: by the evenly laid out bottom in incubator of black soldier flies egg hatching material, supporting gauze on black soldier flies egg hatching material, and black soldier flies ovum is accessed on gauze；The hatching and culture of black soldier flies: it is fallen into after black soldier flies egg hatching inside black soldier flies egg hatching material, culture obtains well-developed black soldier flies larva after 3-5 days, continues to add gradually becoming dark brown stopping to black soldier flies larvae color within pretreated gibberellic acid bacteria residue culture 10-12 days and cultivate；The waste material separation that black soldier flies larva and the culture of black soldier flies larva are generated, obtains black soldier flies maturation larva and black soldier flies larva cultivates waste material.The technical program solves the problems, such as the processing of gibberellic acid bacteria residue, realizes waste utilization.
Description
Technical field
The present invention relates to the technical fields of gibberellic acid bacteria residue processing, utilize black soldier flies larva to red mould more particularly, to a kind of
The method that sour bacteria residue carries out bioconversion.
Background technique
Gibberellin is that a class formation is closely similar, the plant growth regulator being widely present, especially most with gibberellic acid (GA3)
It is important.Gibberellic acid can be used for the overall process of plant growing cycle, can shorten the dormant period of seed, rhizome etc., can induce and blooms, can
It prevents flower, fruit from falling off, can promote fruit maturation and improve yield.The gibberellic acid of China's production at present occupies the exhausted big of world market
Fraction, most of producer are that gibberellic acid is produced using the method for microbial fermentation, and gibberellic acid bacteria residue is Ferment of Gibberellin Acid
The fermentation liquid generated in production process passes through obtained solid residue after plate-frame filtering, it mainly contain remaining culture medium and
A large amount of mycelium, therefore amino acid, a great number of elements (nitrogen, phosphorus, potassium etc.) and microelement rich in gibberellic acid bacteria residue
(iron, manganese, zinc etc.), while also remaining a small amount of gibberellic acid.The traditional treatment method of gibberellic acid bacteria residue be abandon or burn or
It buries, causes the waste of agriculture organic resource, and the method for abandoning and burying will lead to soil eutrophication, the method for burning
Air can be polluted again, so a kind of safe and efficient method need to be found to handle gibberellic acid bacteria residue.
Black soldier flies are the Stratiomyidae insects of a kind of feeding kitchen garbage, animal wastes and animals and plants corpse etc., to the mankind and
It is environmentally friendly.Saprophagous character, breeding is rapid, heavy, feeding habits are extensive, feeding cost is low, generation overlap with seeking for black soldier flies larva
Property preferably, the features such as animal palatability is good and sorption enhanced rate is high, and black soldier flies larva has apparent prepupal period, it can be achieved that longer
The living body of time stores, and prepupa nutritive value is high, therefore black soldier flies larva can be used as a kind of ideal bioconversion material.Heisui River
The not feeding substantially of horsefly adult, does not enter human living space yet, per nearly 1000 of female egg laying amount.Contain in black soldier flies body after drying
Crude protein 45% or so, fatty 35% or so is a kind of ideal insect protein containing 30% or so 18 kinds of amino acid
Feed, and black soldier flies are also rich in antibacterial peptide and probiotics, animal can directly absorb antibacterial peptide and prebiotic by feeding black soldier flies
Bacterium, resistance building, can be remarkably reinforced the disease resistance of shrimps, substantially reduce the shrimp death rate when feeding shrimps, or even reduce
80% death rate.
The existing method using insect processing gibberellic acid bacteria residue is that gibberellin bacteria residue is converted to albumen using housefly to raise
Material, specific operating method is: first being fermented to obtain gibberellin bacteria residue fermentation liquid to gibberellin bacteria residue with a small amount of yeast, and red
The compost of housefly maggot is made in the auxiliary material and water that appropriate amount is added in mycin bacteria residue fermentation liquid, then collects fly in housefly imago cage
The fly blow being collected into simultaneously is linked into compost surface by ovum, and fly blow can hatch fly larvae in compost, and fly larvae can feeding
With digestion compost, through culture after a period of time, fly larvae becomes fly maggot, can be by fly maggot and residual after waiting fly maggot mature
Remaining compost separation, can be respectively as animal feed and organic fertilizer after drying.
The housefly utilized in above-mentioned technical proposal has huge environmental risk, and adult housefly itself is a kind of global
Sanitary insect pest easily propagates a variety of diseases, once mismanagement causes adult housefly to escape during collecting fly blow, then can lead
It causes housefly to spread unchecked, seriously affects people's lives environment, more frighteningly serious harm human health.
Summary of the invention
The purpose of the present invention is to solve deficiencies present in prior art, provide a kind of safe using black
The method that stratiomyiid larva carries out bioconversion to gibberellic acid bacteria residue.
Foregoing invention purpose of the invention has the technical scheme that a kind of utilization black soldier flies larva pair
The method of gibberellic acid bacteria residue progress bioconversion, comprising the following steps:
Step S1, the preparation of black soldier flies egg hatching material: first pre-processing gibberellic acid bacteria residue, then by pretreated gibberellic acid
Bacteria residue is uniformly mixed with auxiliary material, and water is added to adjust water content；
Step S2, the access of black soldier flies ovum: the evenly laid out bottom in incubator of black soldier flies egg hatching material is incubated in black soldier flies ovum
Gauze is supported in material, and black soldier flies ovum is uniformly accessed on gauze；
Step S3, the hatching and culture of black soldier flies: falling into after black soldier flies egg hatching inside black soldier flies egg hatching material, cultivates 3-5 days
After obtain well-developed black soldier flies larva, continue to add pretreated gibberellic acid bacteria residue, be further cultured for 10-12 days to black soldier flies
Larvae color stops culture when gradually becoming dark brown；
Step S4, the post-processing of black soldier flies larva culture: the waste material that black soldier flies larva and the culture of black soldier flies larva generate is separated,
It can be obtained black soldier flies maturation larva and black soldier flies larva culture waste material.
It is rich in organic in the gibberellic acid bacteria residue that is generated in gibberellic acid industrial production by using above-mentioned technical proposal
Object and a small amount of remaining gibberellic acid, when being handled by the way of traditional, on the other hand one side waste of resource can cause environment
Serious pollution, therefore some new methods need to be developed to handle gibberellic acid bacteria residue, by by pretreated gibberellic acid bacteria residue
It is mixed with auxiliary material, and suitable water is added adjusts humidity and black soldier flies egg hatching material for black soldier flies egg hatching, black soldier flies are made
At black soldier flies larva after egg hatching, black soldier flies larva can absorb the nutriment contained in gibberellic acid bacteria residue, to realize to red
The bioconversion of mould acid bacteria residue, had not only solved the pollution problem of gibberellic acid bacteria residue, but also realize the circulation of waste utilization and resource
It utilizes.
The present invention is further arranged to: the preprocessing process in the step S1 include room temperature inactivate remaining gibberellic acid and
Adjustment pH value is at 5-8.
By using above-mentioned technical proposal, remaining gibberellic acid pollutes the environment in gibberellic acid bacteria residue, therefore need to go out
Processing living.The pH value of gibberellic acid bacteria residue after gibberellic acid is inactivated is adjusted to the range of 5-8, and pretreated gibberellic acid bacterium is made
Slag.PH value is too high or too low for that can provide a suitable pH environment when 5-8 for the growth of the larva after black soldier flies egg hatching
PH value will affect the growth of black soldier flies larva, may cause black soldier flies dead larvae or depauperation.
The present invention is further arranged to: the method that the room temperature inactivates remaining gibberellic acid is by the pH value of gibberellic acid bacteria residue
It is adjusted to 10 or more and keeps 6h or more under the conditions of this pH.
By using above-mentioned technical proposal, inactivated since gibberellic acid is unstable under alkaline condition, it is adjustable
The pH value of gibberellic acid bacteria residue, which is in alkaline environment, inactivates remaining gibberellic acid, and operates at normal temperature, compare and its
The method that it heats, this ablation method can be energy saving.
The present invention is further arranged to: the auxiliary material in the step 1 is wheat bran, bran powder, bean cake powder, straw powder, corn
The mixture of one or more of powder, cotton seed hulls, peanut shell powder and sawdust etc..
By using above-mentioned technical proposal, on the one hand the effect for adding auxiliary material is because auxiliary material is in loose shape more, with pre- place
The gibberellic acid bacteria residue managed mixes, and the porousness between adjustable pretreated gibberellic acid bacteria residue to a certain extent may be used
To provide the growth of black soldier flies larva required air, comfortable activity space can also be provided to black soldier flies larva, to accelerate
The growth and development of black soldier flies larva improves black soldier flies larva to the transformation efficiency of gibberellic acid bacteria residue；It on the other hand is for black soldier flies
Growth some necessary nutriments are provided.
The present invention is further arranged to: the weight ratio that pretreated gibberellic acid bacteria residue is mixed with auxiliary material in the step S1
For 4-7:3-6, water content is adjusted to 50-70%.
By using above-mentioned technical proposal, the weight ratio for controlling gibberellic acid bacteria residue and auxiliary material mixing is 4-7:3-6, mixing
The water content of material is 50%-70%, and the growth for the black soldier flies larva after black soldier flies egg hatching provides suitable environment, wherein hatching
The too low efficiency that can reduce black soldier flies larva food of the water content of material, causes black soldier flies larva to be unable to normal development, or even can lead
Blackening stratiomyiid larva prepupa time retardation, so that cultivation cycle be made integrally to extend.
The present invention is further arranged to: black soldier flies egg hatching material exists in incubator bottom tiling thickness in the step S2
10cm hereinafter, the black soldier flies ovum thickness accessed on gauze within 2cm.
By using above-mentioned technical proposal, control thickness that black soldier flies egg hatching material tiles in incubator bottom 10cm with
Under, first is that the black soldier flies larva in black soldier flies egg hatching material is made not suffocate because black soldier flies egg hatching material is too thick, second is that in order to
Black soldier flies egg hatching material is made full use of, if the thickness of black soldier flies egg hatching material is too thick, black soldier flies larva is not easy to contact close to training
The black soldier flies egg hatching material in bottom face is supported, therefore will cause black soldier flies egg hatching material using insufficient.Control the black soldier flies of access
Ovum thickness is the hatching rate in order to improve black soldier flies ovum within 2cm, if black soldier flies ovum is too thick, is on the one hand unfavorable for black soldier flies ovum
Hatching, space is more crowded between the black soldier flies larvas individual and individual of another side hatching, is unfavorable for falling for black soldier flies larva.
The present invention is further arranged to: continuing in the step S3 can be several times when adding pretreated gibberellic acid bacteria residue
Addition, and the piling height of the pretreated gibberellic acid bacteria residue added every time should be not higher than 10cm.
By using above-mentioned technical proposal, the piling height for controlling the pretreated gibberellic acid bacteria residue added every time exists
It is to keep air in the pretreated gibberellic acid bacteria residue of addition sufficient within 10cm, the culture for black soldier flies larva provides
Enough oxygen improves black soldier flies larva to the utilization rate of gibberellic acid bacteria residue.
The present invention is further arranged to: the temperature of black soldier flies egg hatching and the culture of black soldier flies larva in step S2, S3
For 28-35 DEG C, humidity 60-75%.
By using above-mentioned technical proposal, temperature is controlled in 28-35 DEG C, humidity within the scope of 60-75%, to black soldier flies ovum
Hatching and the culture of black soldier flies larva one suitable environment is provided, surviving for black soldier flies larva can be improved in suitable environment
The biotransformation efficiency of rate and black soldier flies larva to gibberellin bacteria residue.
The present invention is further arranged to: the step S4 is using the method separation black soldier flies maturation larva of sieve and black soldier flies
Larva cultivates waste material.
By using above-mentioned technical proposal, after black soldier flies larva is to pretreated gibberellic acid bacteria residue bioconversion, culture
Material is quite loose, the waste material that black soldier flies maturation larva and the culture of black soldier flies larva generate is placed on sieve, by sieve
Sieving, black soldier flies maturation larva stay in above sieve, and black soldier flies larva culture waste material, which is sieved, drops down onto sieve in the following, separate collection sieves
The black soldier flies maturation larva in online face and the black soldier flies larva below sieve cultivate waste material, easy to operate.
The present invention is further arranged to: the black soldier flies maturation larva that the step S4 is obtained can be directly used as protein feed,
Or it is used as additive of protein feed after drying, milling, black soldier flies larva culture waste material can be used as organic fertilizer.
By using above-mentioned technical proposal, nutrition rich in, mainly albumen and rouge in black soldier flies maturation larva body
Fat, therefore black soldier flies maturation larva can directly keep fowls and fish and shrimp, the black soldier flies maturation larva after drying can be used as albumen
Feed addictive.Black soldier flies maturation larva can be prevented by being dried by the way of the drying of lower temperature or natural drying when dry
Intracorporal protein structure is destroyed, if the temperature of drying is excessively high, will lead to albuminous degeneration.And also contain antibacterial in black soldier flies body
Peptide and probiotics, dry temperature is excessively high to make antibacterial peptide and probiotics lose activity.
In conclusion advantageous effects of the invention are as follows: disclosure sets forth black soldier flies larva bioconversion gibberellic acid
The method of bacteria residue, industrial waste is recycled, and is turned waste into wealth, and provides a kind of economic, green, efficiently place for gibberellic acid bacteria residue
Reason method solves the difficult problem of environmental protection that gibberellic acid manufacturing enterprise, China faces.In addition, black soldier flies maturation larva can be directly used for moving
Object feed, or the black soldier flies maturation larva obtained after drying is used as additive of protein feed, black soldier flies culture waste material is available
Make plant organic fertilizer, promotes resource circulation utilization, while also having high economic benefit.
Detailed description of the invention
Fig. 1 is step flow chart of the invention.
Specific embodiment
Below with reference to embodiment, the present invention will be described in detail.
Embodiment 1
A method of bioconversion being carried out to gibberellic acid bacteria residue using black soldier flies larva, as shown in Figure 1, comprising the following steps:
Step S1, the preparation of black soldier flies egg hatching material: the pH value of gibberellic acid bacteria residue is first transferred to 11.0 and is protected under the conditions of this pH
It holds for 24 hours, the pH value of gibberellic acid bacteria residue is transferred to 5 again after gibberellic acid remaining in gibberellic acid bacteria residue all inactivation, pre- place is made
The gibberellic acid bacteria residue managed weighs the pretreated gibberellic acid bacteria residue of 120g and is uniformly mixed with 80g bean cake powder, adds water to adjust aqueous
Amount is 60%, is stirred evenly；
Step S2, black soldier flies egg hatching material the access of black soldier flies ovum: is laid in incubator bottom, the black soldier flies egg hatching of tiling
Material height is 8cm, the metal gauze of one 40 mesh of the place high about 4 ㎝ support above the black soldier flies egg hatching material, in metal gauze
Uniformly access 1g black soldier flies ovum；
Step S3, the hatching and culture of black soldier flies: black soldier flies ovum is in the environment of 28 DEG C of temperature, humidity 60%, hatching culture 5 days,
Period often stirs black soldier flies egg hatching material, then removes metal gauze, directly addition 300g pretreated gibberellic acid bacteria residue,
Continue to cultivate under identical environment.After the change of gibberellic acid bacteria residue is loose, continue to put into pretreated gibberellic acid bacteria residue, repeatedly with this
Pretreated gibberellic acid bacteria residue is added, and as black soldier flies larva grows up, each additive amount gradually amplifies, and continues culture 12
After it, stop culture, black soldier flies larvae color has changed into brown at this time, and food-intake is considerably less, pays attention to from starting to incubate
Change to terminating to cultivate, adds the piling height of pretreated gibberellic acid bacteria residue every time not higher than 10 ㎝；
Step S4, the post-processing of black soldier flies larva culture: black soldier flies maturation larva and black soldier flies larva are cultivated through sieve sieving
Waste material separation obtains black soldier flies maturation larva and black soldier flies larva culture waste material, weighs the matter of black soldier flies maturation larva respectively
Measure M1And the quality m of black soldier flies larva culture waste material2(waste material generated including black soldier flies egg hatching material), the results are shown in Table 1.
Embodiment 2
A method of bioconversion being carried out to gibberellic acid bacteria residue using black soldier flies larva, as shown in Figure 1, comprising the following steps:
Step S1, the preparation of black soldier flies egg hatching material: the pH value of gibberellic acid bacteria residue is first transferred to 11.5 and is protected under the conditions of this pH
18h is held, the pH value of gibberellic acid bacteria residue is transferred to 7 again after gibberellic acid remaining in gibberellic acid bacteria residue all inactivation, pre- place is made
The gibberellic acid bacteria residue managed weighs the pretreated gibberellic acid bacteria residue of 60g and is uniformly mixed with 40g wheat bran, and water is added to adjust water content
It is 60%, stirs evenly；
Step S2, black soldier flies egg hatching material: being laid in the bottom of incubator by the access of black soldier flies ovum, and the black soldier flies ovum of tiling is incubated
Material height is 8cm, the metal gauze of one 40 mesh of the place high about 4 ㎝ support above the black soldier flies egg hatching material, in metal gauze
Upper uniformly access 0.5g black soldier flies ovum；
Step S3, the hatching and culture of black soldier flies: black soldier flies ovum is in the environment of 30 DEG C of temperature, humidity 65%, hatching culture 5 days,
Period often stirs black soldier flies egg hatching material, then removes metal gauze, directly addition 200g pretreated gibberellic acid bacteria residue,
Continue to cultivate under identical environment.After the change of gibberellic acid bacteria residue is loose, continue to put into pretreated gibberellic acid bacteria residue, repeatedly with this
Pretreated gibberellic acid bacteria residue is added, and as black soldier flies larva grows up, each additive amount gradually amplifies, and continues culture 12
After it, stop culture, black soldier flies larvae color has changed into brown at this time, and food-intake is considerably less, pays attention to from starting to incubate
Change to terminating to cultivate, adds the piling height of pretreated gibberellic acid bacteria residue every time not higher than 10 ㎝；
Step S4, the post-processing of black soldier flies larva culture: black soldier flies maturation larva and black soldier flies larva are cultivated through sieve sieving
Waste material separation obtains black soldier flies maturation larva and black soldier flies larva culture waste material, weighs the matter of black soldier flies maturation larva respectively
Measure M1And the quality m of black soldier flies larva culture waste material2(waste material generated including black soldier flies egg hatching material), the results are shown in Table 1.
Embodiment 3
A method of bioconversion being carried out to gibberellic acid bacteria residue using black soldier flies larva, as shown in Figure 1, comprising the following steps:
Step S1, the preparation of black soldier flies egg hatching material: the pH value of gibberellic acid bacteria residue is first transferred to 12 and is kept under the conditions of this pH
The pH value of gibberellic acid bacteria residue is transferred to 6 again after gibberellic acid remaining in gibberellic acid bacteria residue all inactivation, pretreatment is made by 12h
The gibberellic acid bacteria residue crossed weighs the pretreated gibberellic acid bacteria residue of 50g and is uniformly mixed with 50g soybean powder, and water is added to adjust water content
It is 50%, stirs evenly；
Step S2, black soldier flies egg hatching material: being laid in the bottom of incubator by the access of black soldier flies ovum, and the black soldier flies ovum of tiling is incubated
Material height is 8cm, the metal gauze of one 40 mesh of the place high about 4 ㎝ support above the black soldier flies egg hatching material, in metal gauze
Upper uniformly access 0.3g black soldier flies ovum；
Step S3, the hatching and culture of black soldier flies: black soldier flies ovum is in the environment of 33 DEG C of temperature, humidity 70%, hatching culture 4 days,
Period often stirs black soldier flies egg hatching material, then removes metal gauze, directly addition 200g pretreated gibberellic acid bacteria residue,
Continue to cultivate under identical environment.After the change of gibberellic acid bacteria residue is loose, continue to put into pretreated gibberellic acid bacteria residue, repeatedly with this
Pretreated gibberellic acid bacteria residue is added, and as black soldier flies larva grows up, each additive amount gradually amplifies, and continues culture 10
After it, stop culture, black soldier flies larvae color has changed into brown at this time, and food-intake is considerably less, pays attention to from starting to incubate
Change to terminating to cultivate, adds the piling height of pretreated gibberellic acid bacteria residue every time not higher than 10 ㎝；
Step S4, the post-processing of black soldier flies larva culture: black soldier flies maturation larva and black soldier flies larva are cultivated through sieve sieving
Waste material separation obtains black soldier flies maturation larva and black soldier flies larva culture waste material, weighs the matter of black soldier flies maturation larva respectively
Measure M1And the quality m of black soldier flies larva culture waste material2(waste material generated including black soldier flies egg hatching material), the results are shown in Table 1
Embodiment 4
A method of bioconversion being carried out to gibberellic acid bacteria residue using black soldier flies larva, as shown in Figure 1, comprising the following steps:
Step S1, the preparation of black soldier flies egg hatching material: the pH value of gibberellic acid bacteria residue is first transferred to 11 and is kept under the conditions of this pH
For 24 hours, the pH value of gibberellic acid bacteria residue is transferred to 7 again after gibberellic acid remaining in gibberellic acid bacteria residue all inactivation, pretreatment is made
The gibberellic acid bacteria residue crossed weighs the pretreated gibberellic acid bacteria residue of 120g and is uniformly mixed with 80g wheat bran, adds water to adjust water content and is
60%, it stirs evenly；
Step S2, black soldier flies egg hatching material: being laid in the bottom of incubator by the access of black soldier flies ovum, and the black soldier flies ovum of tiling is incubated
Material height is 8cm, the metal gauze of one 40 mesh of the place high about 4 ㎝ support above the black soldier flies egg hatching material, in metal gauze
Upper uniformly access 1g black soldier flies ovum；
Step S3, the hatching and culture of black soldier flies: black soldier flies ovum is in the environment of 35 DEG C of temperature, humidity 75%, hatching culture 3 days,
Period often stirs black soldier flies egg hatching material, then removes metal gauze, directly addition 300g pretreated gibberellic acid bacteria residue,
Continue to cultivate under identical environment.After the change of gibberellic acid bacteria residue is loose, continue to put into pretreated gibberellic acid bacteria residue, repeatedly with this
Pretreated gibberellic acid bacteria residue is added, and as black soldier flies larva grows up, each additive amount gradually amplifies, and continues culture 10
After it, stop culture, black soldier flies larvae color has changed into brown at this time, and food-intake is considerably less, pays attention to from starting to incubate
Change to terminating to cultivate, adds the piling height of pretreated gibberellic acid bacteria residue every time not higher than 10 ㎝；
Step S4, the post-processing of black soldier flies larva culture: black soldier flies maturation larva and black soldier flies larva are cultivated through sieve sieving
Waste material separation obtains black soldier flies maturation larva and black soldier flies larva culture waste material, weighs the matter of black soldier flies maturation larva respectively
Measure M1And the quality m of black soldier flies larva culture waste material2(waste material generated including black soldier flies egg hatching material), the results are shown in Table 1.
Embodiment 5
A method of bioconversion being carried out to gibberellic acid bacteria residue using black soldier flies larva, as shown in Figure 1, comprising the following steps:
Step S1, the preparation of black soldier flies egg hatching material: the pH value of gibberellic acid bacteria residue is first transferred to 10.5 and is protected under the conditions of this pH
48h is held, the pH value of gibberellic acid bacteria residue is transferred to 8 again after gibberellic acid remaining in gibberellic acid bacteria residue all inactivation, pre- place is made
The preparation of black soldier flies larva compost: the gibberellic acid bacteria residue managed weighs the pretreated gibberellic acid bacteria residue of 80g and 120g dregs of beans
Powder is uniformly mixed, and adding water to adjust water content is 70%, is stirred evenly；
Step S2, black soldier flies egg hatching material: being laid in the bottom of incubator by the access of black soldier flies ovum, and the black soldier flies ovum of tiling is incubated
Material height is 8cm, the metal gauze of one 40 mesh of the place high about 4 ㎝ support above the black soldier flies egg hatching material, in metal gauze
Upper uniformly access 1g black soldier flies ovum；
Step S3, the hatching and culture of black soldier flies: black soldier flies ovum is in the environment of 28 DEG C of temperature, humidity 60%, hatching culture 5 days,
Period often stirs black soldier flies egg hatching material, then removes metal gauze, directly addition 300g pretreated gibberellic acid bacteria residue,
Continue to cultivate under identical environment.After the change of gibberellic acid bacteria residue is loose, continue to put into pretreated gibberellic acid bacteria residue, repeatedly with this
Pretreated gibberellic acid bacteria residue is added, and as black soldier flies larva grows up, each additive amount gradually amplifies, and continues culture 12
After it, stop culture, black soldier flies larvae color has changed into brown at this time, and food-intake is considerably less, pays attention to from starting to incubate
Change to terminating to cultivate, adds the piling height of pretreated gibberellic acid bacteria residue every time not higher than 10 ㎝；
Step S4, the post-processing of black soldier flies larva culture: black soldier flies maturation larva and black soldier flies larva are cultivated through sieve sieving
Waste material separation obtains black soldier flies maturation larva and black soldier flies larva culture waste material, weighs the matter of black soldier flies maturation larva respectively
Measure M1And the quality m of black soldier flies larva culture waste material2(waste material generated including black soldier flies egg hatching material), the results are shown in Table 1.
Embodiment 6
A method of bioconversion being carried out to gibberellic acid bacteria residue using black soldier flies larva, as shown in Figure 1, comprising the following steps:
Step S1, the preparation of black soldier flies egg hatching material: the pH value of gibberellic acid bacteria residue is first transferred to 12 and is kept under the conditions of this pH
The pH value of gibberellic acid bacteria residue is transferred to 8 again after gibberellic acid remaining in gibberellic acid bacteria residue all inactivation, pretreatment is made by 12h
The gibberellic acid bacteria residue crossed weighs the pretreated gibberellic acid bacteria residue of 140g and is uniformly mixed with 60g bean cake powder, and water is added to adjust water content
It is 60%, stirs evenly；
Step S2, black soldier flies egg hatching material: being laid in the bottom of incubator by the access of black soldier flies ovum, and the black soldier flies ovum of tiling is incubated
Material height is 8cm, the metal gauze of one 40 mesh of the place high about 4 ㎝ support above the black soldier flies egg hatching material, in metal gauze
Upper uniformly access 1g black soldier flies ovum；
Step S3, the hatching and culture of black soldier flies: black soldier flies ovum is in the environment of 33 DEG C of temperature, humidity 65%, hatching culture 4 days,
Period often stirs black soldier flies egg hatching material, then removes metal gauze, directly addition 300g pretreated gibberellic acid bacteria residue,
Continue to cultivate under identical environment.After the change of gibberellic acid bacteria residue is loose, continue to put into pretreated gibberellic acid bacteria residue, repeatedly with this
Pretreated gibberellic acid bacteria residue is added, and as black soldier flies larva grows up, each additive amount gradually amplifies, and continues culture 12
After it, stop culture, black soldier flies larvae color has changed into brown at this time, and food-intake is considerably less, pays attention to from starting to incubate
Change to terminating to cultivate, adds the piling height of pretreated gibberellic acid bacteria residue every time not higher than 10 ㎝；
Step S4, the post-processing of black soldier flies larva culture: black soldier flies maturation larva and black soldier flies larva are cultivated through sieve sieving
Waste material separation obtains black soldier flies maturation larva and black soldier flies larva culture waste material, weighs the matter of black soldier flies maturation larva respectively
Measure M1And the quality m of black soldier flies larva culture waste material2(waste material generated including black soldier flies egg hatching material), the results are shown in Table 1.
Comparative example 1
A method of bioconversion being carried out to gibberellic acid bacteria residue using black soldier flies larva, as shown in Figure 1, not with embodiment 1
It is with place: does not carry out room temperature inactivation treatment when in step 1 to the pretreatment of gibberellic acid bacteria residue, directly adjusting pH value to 5.
Comparative example 2
A method of bioconversion being carried out to gibberellic acid bacteria residue using black soldier flies larva, as shown in Figure 1, not with embodiment 1
Be with place: the water content of black soldier flies larva compost is 40% in step 1.
Table 1
| Project | M1(g) | m1(g) | M2(g) | m2(g) | S(kg) | G(kg) | Q(g) |
| Embodiment 1 | 1146.86 | 1 | 11000 | 3092.63 | 104.26 | 281.15 | 90.91 |
| Embodiment 2 | 594.56 | 0.5 | 5650 | 1523.20 | 105.23 | 269.59 | 88.50 |
| Embodiment 3 | 362.48 | 0.3 | 3700 | 992.76 | 97.97 | 268.31 | 81.08 |
| Embodiment 4 | 1418.42 | 1 | 12000 | 3456.84 | 118.20 | 288.07 | 83.33 |
| Embodiment 5 | 1196.51 | 1 | 11500 | 3211.49 | 104.04 | 279.26 | 86.96 |
| Embodiment 6 | 1265.78 | 1 | 11700 | 3318.41 | 108.19 | 283.62 | 85.47 |
| Comparative example 1 | 1011.83 | 1 | 10600 | 2700.77 | 95.46 | 254.79 | 94.34 |
| Comparative example 2 | 610.68 | 1 | 7000 | 1623.21 | 87.24 | 231.89 | 142.86 |
Wherein, M1For black soldier flies maturation larva quality, unit g；m1For the quality of the black soldier flies ovum of access, unit g；M2For
The gross mass (gibberellic acid bacteria residue pretreatment front and back moisture content is 65% or so) for the pretreated gibberellic acid bacteria residue being added, it is single
Position is g；m2Waste material quality (waste material generated including black soldier flies egg hatching material) is cultivated for black soldier flies larva to get the organic fertilizer arrived
The quality (moisture content of organic fertilizer is 30% or so, can voluntarily be controlled by different fertilizer moisture requirement) of material, unit g； S,G
The quality for the black soldier flies maturation larva that respectively 1 ton pretreated gibberellic acid bacteria residue culture obtains and the quality of organic fertilizer,
Unit is kg；Q is the quality for the black soldier flies ovum that the pretreated gibberellic acid bacteria residue of every 1 ton of processing needs, unit g.
Through detecting, gibberellic acid is not contained in the black soldier flies larva culture waste material of embodiment 1-6 and the generation of comparative example 2, and it is right
The black soldier flies larva culture waste material that ratio 1 obtains still remains gibberellic acid since gibberellic acid bacteria residue does not carry out inactivation treatment,
Therefore the black soldier flies larva culture waste material that embodiment 1-6 and comparative example 2 obtain can be used as organic fertilizer, and what comparative example 1 obtained
Black soldier flies larva culture waste material cannot act as organic fertilizer use；S is bigger, illustrates 1 ton of pretreated gibberellic acid bacteria residue culture
The yield of obtained black soldier flies maturation larva is higher, and G is bigger, illustrates that 1 ton of pretreated gibberellic acid bacteria residue culture obtained has
Machine fertilizer is more, and Q is smaller, illustrates that the quality for the black soldier flies ovum that the pretreated gibberellic acid bacteria residue of every 1 ton of processing needs is fewer, table
S, the G of S, G of embodiment 1-6 than comparative example 2 are big in 1, and the Q of embodiment 1-6 is significantly less than the Q of comparative example 2, in summary
Illustrate that the technical program is substantially better than comparative example.
The above is only a preferred embodiment of the present invention, protection scope of the present invention is not limited merely to above-mentioned implementation
Example, all technical solutions belonged under thinking of the present invention all belong to the scope of protection of the present invention.It should be pointed out that for the art
Those of ordinary skill for, several improvements and modifications without departing from the principles of the present invention, these improvements and modifications
It should be regarded as protection scope of the present invention.
Claims (10)
1. a kind of method for carrying out bioconversion to gibberellic acid bacteria residue using black soldier flies larva, it is characterised in that: including following step
It is rapid:
Step S1, the preparation of black soldier flies egg hatching material: first pre-processing gibberellic acid bacteria residue, then by pretreated gibberellic acid
Bacteria residue is uniformly mixed with auxiliary material, and water is added to adjust water content；
Step S2, the access of black soldier flies ovum: the evenly laid out bottom in incubator of black soldier flies egg hatching material is incubated in black soldier flies ovum
Gauze is supported in material, and black soldier flies ovum is uniformly accessed on gauze；
Step S3, the hatching and culture of black soldier flies: falling into after black soldier flies egg hatching inside black soldier flies egg hatching material, cultivates 3-5 days
After obtain well-developed black soldier flies larva, continue to add pretreated gibberellic acid bacteria residue, be further cultured for 10-12 days to black soldier flies
Larvae color stops culture when gradually becoming dark brown；
Step S4, the post-processing of black soldier flies larva culture: the waste material that black soldier flies larva and the culture of black soldier flies larva generate is separated,
It can be obtained black soldier flies maturation larva and black soldier flies larva culture waste material.
2. a kind of method that bioconversion is carried out to gibberellic acid bacteria residue using black soldier flies larva according to claim 1,
Be characterized in that: the preprocessing process in the step S1 includes that the remaining gibberellic acid of room temperature inactivation and adjustment pH value are at 5-
8。
3. a kind of method that bioconversion is carried out to gibberellic acid bacteria residue using black soldier flies larva according to claim 2,
Be characterized in that: the method that the room temperature inactivates remaining gibberellic acid is the pH value of gibberellic acid bacteria residue to be adjusted to 10 or more and in this pH
Under the conditions of keep 6h or more.
4. a kind of method that bioconversion is carried out to gibberellic acid bacteria residue using black soldier flies larva according to claim 1,
Be characterized in that: the auxiliary material in the step S1 is wheat bran, bran powder, bean cake powder, straw powder, corn flour, cotton seed hulls, peanut shell powder
With the mixture of one or more of sawdust etc..
5. a kind of method that bioconversion is carried out to gibberellic acid bacteria residue using black soldier flies larva according to claim 1 or 3,
It is characterized by: the weight ratio that gibberellic acid bacteria residue pretreated in the step S1 is mixed with auxiliary material is 4-7:3-6, water content
It is adjusted to 50-70%.
6. a kind of method that bioconversion is carried out to gibberellic acid bacteria residue using black soldier flies larva according to claim 1,
Be characterized in that: in the step S2 black soldier flies egg hatching material incubator bottom tiling thickness in 10cm hereinafter, being accessed on gauze
Black soldier flies ovum thickness within 2cm.
7. a kind of method that bioconversion is carried out to gibberellic acid bacteria residue using black soldier flies larva according to claim 1,
It is characterized in that: continuing to add several times when adding pretreated gibberellic acid bacteria residue in the step S3, and add every time
The piling height of pretreated gibberellic acid bacteria residue should be not higher than 10cm.
8. a kind of method that bioconversion is carried out to gibberellic acid bacteria residue using black soldier flies larva according to claim 1,
Be characterized in that: in step S2, S3 black soldier flies egg hatching and black soldier flies larva culture temperature be 28-35 DEG C, humidity 60-
75%.
9. a kind of method that bioconversion is carried out to gibberellic acid bacteria residue using black soldier flies larva according to claim 1,
Be characterized in that: the step S4 cultivates waste material using the method separation black soldier flies maturation larva of sieve and black soldier flies larva.
10. a kind of method that bioconversion is carried out to gibberellic acid bacteria residue using black soldier flies larva according to claim 1,
Be characterized in that: the black soldier flies maturation larva that the step S4 is obtained can be directly used as protein feed, or be used as after drying, milling
Additive of protein feed, black soldier flies larva culture waste material can be used as organic fertilizer.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110720435A (en) * | 2019-11-14 | 2020-01-24 | 自然创造(北京)科技有限公司 | Breeding method of hermetia illucens, selenium-rich insect protein source feed, selenium-rich organic fertilizer and processing method thereof |
| CN115057729A (en) * | 2022-06-20 | 2022-09-16 | 龙蟒大地农业有限公司 | Method for transforming and utilizing gibberellin fungi residues |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101889629A (en) * | 2010-06-13 | 2010-11-24 | 中山大学 | Method for processing food waste by using black soldier fly larvae and material formula |
| CN103918612A (en) * | 2014-04-11 | 2014-07-16 | 华中农业大学 | Method for utilizing larvae-treated gibberellin fermentation residues to produce insect protein and organic fertilizers |
| FR3013561A1 (en) * | 2013-11-28 | 2015-05-29 | Pierre Furtos | DEVICE FOR BREEDING FLYING INSECTS SUCH AS HERMETIA ILLUCENS |
| CN108033847A (en) * | 2018-01-09 | 2018-05-15 | 江西新瑞丰生化股份有限公司 | A kind of gibberellin bacteria residue composite microbiological fertilizer and preparation method thereof |
-
2018
- 2018-12-28 CN CN201811654063.3A patent/CN109619045B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101889629A (en) * | 2010-06-13 | 2010-11-24 | 中山大学 | Method for processing food waste by using black soldier fly larvae and material formula |
| FR3013561A1 (en) * | 2013-11-28 | 2015-05-29 | Pierre Furtos | DEVICE FOR BREEDING FLYING INSECTS SUCH AS HERMETIA ILLUCENS |
| CN103918612A (en) * | 2014-04-11 | 2014-07-16 | 华中农业大学 | Method for utilizing larvae-treated gibberellin fermentation residues to produce insect protein and organic fertilizers |
| CN108033847A (en) * | 2018-01-09 | 2018-05-15 | 江西新瑞丰生化股份有限公司 | A kind of gibberellin bacteria residue composite microbiological fertilizer and preparation method thereof |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110720435A (en) * | 2019-11-14 | 2020-01-24 | 自然创造(北京)科技有限公司 | Breeding method of hermetia illucens, selenium-rich insect protein source feed, selenium-rich organic fertilizer and processing method thereof |
| CN115057729A (en) * | 2022-06-20 | 2022-09-16 | 龙蟒大地农业有限公司 | Method for transforming and utilizing gibberellin fungi residues |
| CN115057729B (en) * | 2022-06-20 | 2023-12-22 | 龙蟒大地农业有限公司 | Gibberellin fungus dreg conversion and utilization method |
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