CN103865800B - 一株产巴卡亭iii的红豆杉内生真菌 - Google Patents

一株产巴卡亭iii的红豆杉内生真菌 Download PDF

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CN103865800B
CN103865800B CN201310243089.XA CN201310243089A CN103865800B CN 103865800 B CN103865800 B CN 103865800B CN 201310243089 A CN201310243089 A CN 201310243089A CN 103865800 B CN103865800 B CN 103865800B
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baccatin iii
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mycelia
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CN103865800A (zh
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简在友
孟丽
王鸿升
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Henan Institute of Science and Technology
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Abstract

本专利为一株产巴卡亭III的红豆杉内生真菌，种类是Diaporthe？phaseolorum(Cooke&Ellis)Sacc.用液体PDA培养基在室温条件下按照每分钟80-100转的震荡培养方式培养这种菌8-10天后，包含培养液和菌丝的培养物中巴卡亭III(baccatin？III)含量达到0.8mg/L以上。该菌株液体培养产生的巴卡亭III能够用于人工合成紫杉醇。

Description

一株产巴卡亭III的红豆杉内生真菌
技术领域：本专利属于生物工程技术领域，具体地说属于一个真菌菌株，可用于生产巴卡亭III，进而由此人工合成紫杉醇。
背景技术：紫杉醇是治疗癌症的一种有效天然化学成分，但紫杉醇在红豆杉体内含量很低，在万分之一以下。产紫杉醇的红豆杉植物资源十分有限，红豆杉已被列为国家保护植物，不能任意砍伐；栽培红豆杉生长也十分缓慢，还存在着紫杉醇含量更低的问题。目前已有人从红豆杉中分离得到产紫杉醇的内生真菌，不过产率很低，还达不到工业化生产水平。紫杉醇合成的一种前体物是巴卡亭III，由巴卡亭III可方便地人工合成紫杉醇，但巴卡亭III在红豆杉体内含量也很低，生产廉价的巴卡亭III是解决紫杉醇药源紧缺问题的一个有效技术措施。
发明内容：从太行山区的南方红豆杉植物体内分离得到一株产巴卡亭III(baccatinIII)的内生菌，经鉴定种名为Diaporthephaseolorum(Cooke&Ellis)Sacc。该菌种已于2012年12月6日保藏于中国微生物菌种保藏管理委员会普通微生物中心，保藏单位地址为北京市朝阳区北辰西路1号院3号，保藏编号为CGMCCNo.6952。用液体PDA培养基在室温条件下按照每分钟80-100转的震荡培养方式培养这种菌8-10天后，包含培养液和菌丝的培养物中巴卡亭III含量达到0.8mg/L以上。该菌株液体培养产生的巴卡亭III能够用于人工合成紫杉醇。
具体实施方式：先制备出本专利菌株的种子液，再把种子液用液体PDA培养基震荡培养(80-100转/分钟)8-10天后，从培养物中提取巴卡亭III。

Claims (3)

1.一株产巴卡亭III的红豆杉内生真菌，菌株保藏号为CGMCCNo.6952，菌种为Diaporthephaseolorum(Cooke&Ellis)Sacc，其特征在于能够产生化学物质巴卡亭III(baccatinIII)。
2.根据权利要求1所述的产巴卡亭III菌株，其特征在于用液体PDA培养基在室温条件下按照每分钟80-100转的震荡培养方式培养这种菌8-10天后，包含培养液和菌丝的培养物中巴卡亭III含量达到0.8mg/L以上。
3.根据权利要求1所述的产巴卡亭III菌株，其特征在于这种真菌为从红豆杉植物体内分离得到的内生菌，菌丝在PDA培养基上室温条件下能够正常生长，在PDA培养基上生长的菌丝白色，菌落呈放射状，有基生菌丝和气生菌丝，气生菌丝稍稀，菌落贴着培养基。
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CN106967623A (zh) * 2017-05-17 2017-07-21 曹军卫 一株产紫杉烷类化合物巴卡亭ⅲ的黑曲霉及其应用

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WO2016082186A1 (zh) * 2014-11-28 2016-06-02 天津工业大学 一种具有细胞毒活性的组合物及其制备方法和应用
CN104726345B (zh) * 2015-02-07 2020-12-04 河南科技学院 一组高产巴卡亭ⅲ组合菌株及生产巴卡亭ⅲ的方法
CN104975054A (zh) * 2015-06-25 2015-10-14 河南科技学院 一种提高真菌产10-去乙酰巴卡亭iii产率的方法
CN104975053B (zh) * 2015-06-25 2020-12-01 河南科技学院 一种提高真菌产巴卡亭iii产率的氧化环境发酵方法
CN105567574B (zh) * 2016-01-08 2019-03-05 河南科技学院 一株高产紫杉醇的红豆杉内生真菌

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