CN103828711A - Marking method for hybrid seed production of hot peppers - Google Patents
Marking method for hybrid seed production of hot peppers Download PDFInfo
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- CN103828711A CN103828711A CN201410101133.8A CN201410101133A CN103828711A CN 103828711 A CN103828711 A CN 103828711A CN 201410101133 A CN201410101133 A CN 201410101133A CN 103828711 A CN103828711 A CN 103828711A
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Abstract
The invention relates to the technical field of hybrid seed production of plants, and particularly discloses a marking method for the hybrid seed production of hot peppers. The method comprises the following steps: (1) determining the cross pollination period of the hot peppers; (2) marking female parent plants selected for the hybrid seed production; (3) collecting pollen of the cross-breeding male parents; (4) performing emasculation cross-breeding and flower sleeving isolation; (5) marking cross-breeding flower positions; (6) marking seed fruits; and (7) picking fruits and processing. According to the marking method for the hybrid seed production of the hot peppers, the mixing of fruits with different varieties during picking can be completely eradicated, the purity of seeds can be ensured, and the accuracy of experimental research in a later period and the consistency of production seeds can be ensured.
Description
Technical field
The present invention relates to plant hybridization seeding technique field, is a kind of capsicum hybrid seeding labeling method specifically.
Background technology
Capsicum, is a kind of Chang Yihua crop, and androgyny, mostly generally is selfing, and simultaneously, because of anemophily and the impact of entomophila factor, different colored hybrid rate is also higher, can reach 15～20% and more than.Produce with on planting in capsicum, present stage adopts the conventional polycross kind use conversion of planting except green pepper really has indivedual purposes.Produce in capsicum, test and carrying out in hybrid seeding for multiple merit gene transformation polymerizations, other allocarpy of also sneaking into selfed seed fruit and producing because of anemophily or entomophila factor because of the green pepper fruit of now often gathering in the time gathering kind by labeling method defect, and cannot check in the time of seed collecting species test, bringing to produce uses variet complexity, the impact of planting and test the production of hybrid seeds to produce and experimental study, particularly carry out hybrid seeding in the capsicum test production of hybrid seeds and excellent genes transformation, sometimes because the fruit error of gathering is brought and had a strong impact on to experimental study.The labeling method of capsicum hybrid seeding is summed up in research, stops the variet complexity of hybrid seeding, ensures the purity of seed, can guarantee the later stage produce with kind uniformity and the correctness of experimental study, can accelerate to advance the sustainable development of capsicum industry.
Summary of the invention
The object of this invention is to provide a kind of capsicum hybrid seeding labeling method, variet complexity when it can finally be stopped capsicum hybrid seeding and gathers kind, ensure the purity of seed, guarantee the later stage produce with kind uniformity and the correctness of experimental study.
For achieving the above object, the technical solution used in the present invention is: a kind of capsicum hybrid seeding labeling method, comprises the steps:
(1) determine capsicum hybridization pollination period;
(2) the selected maternal plant mark of hybrid seeding;
(3) parent sire of hybrid pigs pollen gathers;
(4) emasculation hybridization and the isolation of cover flower;
(5) hybridization flower position mark;
(6) plant fruit mark;
(7) fruit picking and processing.
Further, described step (1) determines that capsicum hybridization pollination is that flowering stage of 3rd～6 flowers of capsicum maternal plant is hybridization pollination period period.
Further, the maternal plant mark that described step (2) hybrid seeding is selected, comprises the steps: in the pollinated stage of step (1), hangs and perform mark on the maternal plant branch of the production of hybrid seeds of selecting and remain with colored drop.
Further, described step (3) parent sire of hybrid pigs pollen gathers, comprise the steps: that the planned production of hybrid seeds is combined in that 1d before pollination plucks maturation afternoon but the male parent flower pesticide of also not blooming, there is the template/bag of male parent title to wrap with mark, being placed in dry place, indoor calm cool place spreads out, allow and gather pollen (medicine of also can that morning picking flowers of pollination in afternoon, gather pollen after loose powder noon) after flower pesticide nature loose powder.
Further, described step (4) emasculation hybridization and the isolation of cover flower, comprise the steps: petal and androecium ripe on plant selected step (2) but that do not bloom to remove, paternal pollen step (4) being gathered by production of hybrid seeds plan is coated on the chapiter after emasculation (female parent be male sterile do not exist emasculation directly to pollinate) with banister bruss, bennet place paper using adhesive plaster after pollination is trumpet type and clings, pollination flower is wrapped in loudspeaker, prevent that (female parent is male sterile because of the pollination of entomophila or anemophily a string of flowers, do not plant can isolating without adhesive plaster of other capsicum material with field), and record pollination situation.
Further, described step (5) hybridization flower position mark, comprises the steps:
1. the leaf hand that maternal individual plant is only hybridized the leaf position, the pollination by step (4) flower place of 1 male parent is pinched half, stays half leaf on plant, and indicate hybrid combination title with marking pen on pollination plant drop;
2. the leaf hand that maternal individual plant is hybridized the leaf position, the pollination by step (4) flower place of multiple male parents is pinched half, the hybrid combination code name that after being numbered by parent with gel ink pen mark on the half sheet blade face of staying on plant, double figures forms.
Further, described step (6) is planted fruit mark, comprises the steps:
1. maternal individual plant is only hybridized 1 male parent, and after pollination, 8～10d smears mark with marking pen by the young fruit of step (5) half leaf mark positions on carpopodium;
2. maternal individual plant is hybridized multiple male parents, and the moulding fruit that step (5) mark position is entered green mature by 15～20d after pollination contrasts the hybrid combination code name marking pen of staying mark on first sheet leaf of plant at its fruit face mark hybrid combination code name.
Further, (7) fruit picking of described step and processing, comprise the steps: that pollination flower is born fruit after maturation after step (4) emasculation hybridization, according to the production of hybrid seeds situation of hybrid combination, prepare to gather bag, indicate the number-mark of production of hybrid seeds combination, check the number and pluck hybrid combination green pepper fruit, green pepper fruit is transported back and indoorly collects seed after rechecking mark, institute collects seed son eluriate impurity elimination be placed on nothing by force daylight directly shine aeration-drying place and dry in the shade, obtain hybrid seed, complete production of hybrid seeds process.
Useful technique effect of the present invention is: the seed that adopts capsicum crossbreed fruit that labeling method of the present invention selects and remain to gather, can stop the variet complexity in capsicum hybrid seeding process, ensure the purity of seed, guarantee the uniformity of later stage production use kind and the correctness of experimental study.
Embodiment
Below in conjunction with embodiment, the present invention is described further;
Embodiment 1
Capsicum hybrid seeding labeling method, comprises the steps:
1), determine capsicum hybridization pollination best period: spend flower in full bloom to begin as the best initial time of hybridization pollination for the 3rd the male sterile maternal material plant of capsicum " 9537 ".
2), the selected maternal plant mark of hybrid seeding: perform mark with the hanging of colored drop on the branch at " 9537 " obvious place of maternal plant of the production of hybrid seeds of selecting and remain, indicate maternal numbering " 9537 " with marking pen on drop.
3), parent sire of hybrid pigs pollen gathers: the planned production of hybrid seeds is combined in that 1d before pollination plucks maturation afternoon but the flower pesticide of the male parent " 4536 " of also not blooming, with mark " 4536 " title template (bag) wrap, be placed in dry place, indoor calm cool place and spread out, allow after flower pesticide nature loose powder and gather pollen.
4), pollination hybridization and the isolation of cover flower: " 4536 " paternal pollen gathering is coated in banister bruss on the chapiter of selected " 9537 " maternal plant mature flower, bennet place paper using adhesive plaster after pollination is trumpet type and clings, pollination flower is wrapped in loudspeaker, prevent because of entomophila or the pollination of anemophily a string of flowers; Be implanted with male parent can isolating without bagging of other capsicum material in addition with field without kind.On minute book, record pollination situation.
5), hybridization flower position mark: the leaf of leaf position, pollination flower place is pinched to half with hand, stay half leaf on plant, and indicate hybrid combination title " 9537 × 4536 " with marking pen on pollination plant drop.
6), plant fruit mark: 8d smears mark with marking pen by the young fruit of half leaf mark position on carpopodium after pollination.
7), fruit picking and processing: bear fruit after maturation at hybridization pollination flower, prepare fruit picking bag, indicate production of hybrid seeds combination title " 9537 × 4536 " mark, check the number and pluck markd green pepper fruit on carpopodium, the green pepper fruit of gathering is collected seed after transporting back and rechecking mark after indoor, institute collects seed son eluriate impurity elimination be placed on nothing by force daylight directly shine aeration-drying place and dry in the shade.Obtain the seed of hybrid combination " 9537 × 4536 ".
Embodiment 2
Capsicum hybrid seeding labeling method, comprises the steps:
1), determine capsicum hybridization pollination best period: spend flower in full bloom to begin as the hybridization pollination best start phase the 4th of capsicum " 4631 " maternal plant.
2), the selected maternal plant mark of hybrid seeding: perform mark with the hanging of colored drop on the branch at " 4631 " obvious place of maternal plant of the production of hybrid seeds of selecting and remain, indicate maternal numbering " 4631 " with marking pen on drop.
3), parent sire of hybrid pigs pollen gathers: the planned production of hybrid seeds is combined in that 1d before pollination plucks maturation afternoon but the flower pesticide of the male parent " 5326 " of also not blooming, there is the template (bag) of " 5326 " male parent title to wrap with mark, be placed in dry place, indoor calm cool place and spread out, allow after flower pesticide nature loose powder and gather pollen.
4), emasculation hybridization and the isolation of cover flower: petal and androecium ripe on selected " 4631 " maternal plant but that do not bloom are removed, " 5326 " paternal pollen gathering is coated on the chapiter after emasculation with banister bruss, then the bennet place paper using adhesive plaster after pollination is trumpet type and clings, pollination flower is wrapped in loudspeaker, prevent because of entomophila or the pollination of anemophily a string of flowers.On minute book, record pollination situation.
5), maternal individual plant is only hybridized the hybridization flower position mark of 1 male parent: the leaf hand of leaf position, pollination flower place is pinched to half, stay half leaf on plant, and indicate hybrid combination title " 4631 × 5326 " with marking pen on pollination plant drop.
6), plant fruit mark: 10d smears mark with marking pen by the young fruit of half leaf mark position on carpopodium after pollination.
7), fruit picking and processing: bear fruit after maturation at hybridization pollination flower, the preparation bag of gathering, indicate production of hybrid seeds combination title " 4631 × 5326 " mark, check the number and pluck markd green pepper fruit on carpopodium, the green pepper fruit of gathering is collected seed after transporting back and rechecking mark after indoor, institute collects seed son eluriate impurity elimination be placed on nothing by force daylight directly shine aeration-drying place and dry in the shade.Obtain the seed of hybrid combination " 4631 × 5326 ".
Embodiment 3
Capsicum hybrid seeding labeling method, comprises the steps:
1), determine capsicum hybridization pollination best period: spending flower in full bloom to begin the 5th of capsicum " 4637 " maternal plant is hybridization pollination best start period.
2), the selected maternal plant mark of hybrid seeding: perform mark with the hanging of colored drop on the branch at " 4637 " obvious place of maternal plant of the production of hybrid seeds of selecting and remain, indicate maternal numbering " 4637 " with marking pen on drop.
3), parent sire of hybrid pigs pollen gathers: the planned production of hybrid seeds is combined in that 1d before pollination plucks maturation afternoon but the flower pesticide of the male parent " 4307 " of also not blooming, there is the template (bag) of " 4307 " male parent title to wrap with mark, be placed in dry place, indoor calm cool place and spread out, allow after flower pesticide nature loose powder and gather pollen.
4), emasculation hybridization and the isolation of cover flower: petal and androecium ripe on selected " 4637 " maternal plant but that do not bloom are removed, " 4307 " paternal pollen gathering is coated on the chapiter after emasculation with banister bruss, then the bennet place paper using adhesive plaster after pollination is trumpet type and clings, pollination flower is wrapped in loudspeaker, prevent because of entomophila or the pollination of anemophily a string of flowers.On minute book, record pollination situation.
5), maternal individual plant is hybridized the hybridization flower position mark of multiple male parents: the leaf hand of leaf position, pollination flower place is pinched to half, stay half leaf on plant, and indicate the hybrid combination numbering " 3707 " by double figures forms after hybrid strain numbering with gel ink pen on the half sheet blade face of staying on plant.
6), plant fruit mark: the moulding fruit that half leaf mark position is entered green mature by 15d after pollination contrasts the hybrid combination code name of staying mark on first sheet blade face of plant, uses marking pen at its fruit face mark hybrid combination code name " 3707 ".
7), fruit picking and processing: bear fruit after maturation at hybridization pollination flower, the preparation bag of gathering, indicate production of hybrid seeds combination code name " assorted 3707 " mark, check the number and pluck markd green pepper fruit on fruit face, the green pepper fruit of gathering is collected seed after transporting back and rechecking mark after indoor, institute collects seed son eluriate impurity elimination be placed on nothing by force daylight directly shine aeration-drying place and dry in the shade.Obtain the seed of hybrid combination " assorted 3707 ".
Embodiment 4
Capsicum hybrid seeding labeling method, comprises the steps:
1), determine capsicum hybridization pollination best period: spending flower in full bloom to begin the 6th of capsicum " 4637 " maternal plant is hybridization pollination best start period.
2), the selected maternal plant mark of hybrid seeding: perform mark with the hanging of colored drop on the branch at " 4637 " obvious place of maternal plant of the production of hybrid seeds of selecting and remain, indicate maternal numbering " 4637 " with marking pen on drop.
3), parent sire of hybrid pigs pollen gathers: the planned production of hybrid seeds is combined in that 1d before pollination plucks maturation afternoon but the flower pesticide of the male parent " 5412 " of also not blooming, there is the template (bag) of " 5412 " male parent title to wrap with mark, be placed in dry place, indoor calm cool place and spread out, allow after flower pesticide nature loose powder and gather pollen.
4), emasculation hybridization and the isolation of cover flower: petal and androecium ripe on selected " 4637 " maternal plant but that do not bloom are removed, " 5412 " paternal pollen gathering is coated on the chapiter after emasculation with banister bruss, then the bennet place paper using adhesive plaster after pollination is trumpet type and clings, pollination flower is wrapped in loudspeaker, prevent because of entomophila or the pollination of anemophily a string of flowers.On minute book, record pollination situation.
5), maternal individual plant is hybridized the hybridization flower position mark of multiple male parents: the leaf hand of leaf position, pollination flower place is pinched to half, stay half leaf on plant, and indicate the hybrid combination numbering " 3712 " by double figures forms after hybrid strain numbering with gel ink pen on the half sheet blade face of staying on plant.
6), plant fruit mark: the moulding fruit that half leaf mark position is entered green mature by 20d after pollination contrasts the hybrid combination code name of staying mark on first sheet blade face of plant, uses marking pen at its fruit face mark hybrid combination code name " 3712 ".
7), fruit picking and processing: bear fruit after maturation at hybridization pollination flower, the preparation bag of gathering, indicate production of hybrid seeds combination code name " assorted 3712 " mark, check the number and pluck markd green pepper fruit on fruit face, the green pepper fruit of gathering is collected seed after transporting back and rechecking mark after indoor, institute collects seed son eluriate impurity elimination be placed on nothing by force daylight directly shine aeration-drying place and dry in the shade.Obtain the seed of hybrid combination " assorted 3712 ".
The foregoing is only preferred embodiment of the present invention, in order to limit the present invention, within the spirit and principles in the present invention not all, any amendment of doing, be equal to replacement, improvement etc., within all should being included in protection scope of the present invention.
Claims (8)
1. capsicum hybrid seeding labeling method, is characterized in that, comprises the steps:
(1) determine capsicum hybridization pollination period;
(2) the selected maternal plant mark of hybrid seeding;
(3) parent sire of hybrid pigs pollen gathers;
(4) emasculation hybridization and the isolation of cover flower;
(5) hybridization flower position mark;
(6) plant fruit mark;
(7) fruit picking and processing.
2. capsicum hybrid seeding labeling method according to claim 1, is characterized in that, described step (1) determines that capsicum hybridization pollination is that flowering stage of 3rd～6 flowers of capsicum maternal plant is hybridization pollination period period.
3. capsicum hybrid seeding labeling method according to claim 1, it is characterized in that, the maternal plant mark that described step (2) hybrid seeding is selected, comprises the steps: in the pollinated stage of step (1), hangs and perform mark on the maternal plant branch of the production of hybrid seeds of selecting and remain with colored drop.
4. capsicum hybrid seeding labeling method according to claim 1, it is characterized in that, described step (3) parent sire of hybrid pigs pollen gathers, comprise the steps: that the planned production of hybrid seeds is combined in that 1d before pollination plucks maturation afternoon but the male parent flower pesticide of also not blooming, there is the template/bag of male parent title to wrap with mark, be placed in dry place, indoor calm cool place and spread out, allow after flower pesticide nature loose powder and gather pollen.
5. capsicum hybrid seeding labeling method according to claim 1, it is characterized in that, described step (4) emasculation hybridization and the isolation of cover flower, comprise the steps: petal and androecium ripe on plant selected step (2) but that do not bloom to remove, paternal pollen step (4) being gathered by production of hybrid seeds plan is coated on the chapiter after emasculation with banister bruss, bennet place paper using adhesive plaster after pollination is trumpet type and clings, pollination flower is wrapped in loudspeaker, prevent because of entomophila or the pollination of anemophily a string of flowers, and record pollination situation.
6. capsicum hybrid seeding labeling method according to claim 1, is characterized in that, described step (5) hybridization flower position mark, comprises the steps:
1. the leaf hand that maternal individual plant is only hybridized the leaf position, the pollination by step (4) flower place of 1 male parent is pinched half, stays half leaf on plant, and indicate hybrid combination title with marking pen on pollination plant drop;
2. the leaf hand that maternal individual plant is hybridized the leaf position, the pollination by step (4) flower place of multiple male parents is pinched half, the hybrid combination code name that after being numbered by parent with gel ink pen mark on the half sheet blade face of staying on plant, double figures forms.
7. capsicum hybrid seeding labeling method according to claim 1, is characterized in that, described step (6) is planted fruit mark, comprises the steps:
1. maternal individual plant is only hybridized 1 male parent, and after pollination, 8～10d smears mark with marking pen by the young fruit of step (5) half leaf mark positions on carpopodium;
2. maternal individual plant is hybridized multiple male parents, and the moulding fruit that step (5) mark position is entered green mature by 15～20d after pollination contrasts the hybrid combination code name marking pen of staying mark on first sheet leaf of plant at its fruit face mark hybrid combination code name.
8. capsicum hybrid seeding labeling method according to claim 1, it is characterized in that, (7) fruit picking of described step and processing, comprise the steps: that pollination flower is born fruit after maturation after step (4) emasculation hybridization, according to the production of hybrid seeds situation of hybrid combination, the preparation bag of gathering, indicate the number-mark of production of hybrid seeds combination, check the number and pluck hybrid combination green pepper fruit, green pepper fruit is transported back and indoorly collects seed after rechecking mark, the son of collecting seed is eluriated impurity elimination and is placed on and directly shines aeration-drying place without strong daylight and dry in the shade, and obtains hybrid seed, completes production of hybrid seeds process.
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| CN201410101133.8A CN103828711B (en) | 2014-03-14 | 2014-03-14 | Capsicum hybrid seeding labeling method |
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| CN201410101133.8A CN103828711B (en) | 2014-03-14 | 2014-03-14 | Capsicum hybrid seeding labeling method |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1176736A (en) * | 1997-09-12 | 1998-03-25 | 开封市蔬菜科学研究所 | Method for breeding hybridized cayence pepper |
| KR20050004981A (en) * | 2003-07-01 | 2005-01-13 | 재단법인서울대학교산학협력재단 | Method for breeding a hybrid pepper using interspecific hybridization |
| CN1781358A (en) * | 2004-11-29 | 2006-06-07 | 中国农业大学 | Method for producing hot pepper hybrid seed and appraising purity |
| CN101011022A (en) * | 2007-01-30 | 2007-08-08 | 广东省农业科学院蔬菜研究所 | Seed selection method of capsicum variety |
-
2014
- 2014-03-14 CN CN201410101133.8A patent/CN103828711B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1176736A (en) * | 1997-09-12 | 1998-03-25 | 开封市蔬菜科学研究所 | Method for breeding hybridized cayence pepper |
| KR20050004981A (en) * | 2003-07-01 | 2005-01-13 | 재단법인서울대학교산학협력재단 | Method for breeding a hybrid pepper using interspecific hybridization |
| CN1781358A (en) * | 2004-11-29 | 2006-06-07 | 中国农业大学 | Method for producing hot pepper hybrid seed and appraising purity |
| CN101011022A (en) * | 2007-01-30 | 2007-08-08 | 广东省农业科学院蔬菜研究所 | Seed selection method of capsicum variety |
Non-Patent Citations (2)
| Title |
|---|
| 余康宁等: "辣椒03-10-6组合杂交制种技术规程", 《农技服务》 * |
| 吕中华等: "改良早丰辣椒杂交制种技术研究", 《西南农业学报》 * |
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Inventor after: Yang Hong Inventor after: Jiang Hong Inventor after: Lai Wei Inventor after: Liu Chongzheng Inventor after: Su Dan Inventor after: Mou Yumei Inventor before: Yang Hong Inventor before: Jiang Hong |
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