CN105706907A - Rapid selfing isolation method for purple pod peppers in greenhouse - Google Patents
Rapid selfing isolation method for purple pod peppers in greenhouse Download PDFInfo
- Publication number
- CN105706907A CN105706907A CN201610067690.1A CN201610067690A CN105706907A CN 105706907 A CN105706907 A CN 105706907A CN 201610067690 A CN201610067690 A CN 201610067690A CN 105706907 A CN105706907 A CN 105706907A
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- China
- Prior art keywords
- flower
- purple
- selfing
- capsicum annum
- annum fasciculatum
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Botany (AREA)
- Developmental Biology & Embryology (AREA)
- Environmental Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention discloses a rapid selfing isolation method for purple pod peppers in a greenhouse, and belongs to the technical field of plant breeding. The method comprises the steps that purple pod pepper selfing individual plants are selected and marked with hanging tags; flowers to be opened in the marked individual plants are selected; the flowers are wrapped by sticky label paper, and the upper ends and the lower ends of the flowers are clenched; the flowers are marked with hanging tags; fruits marked with the hanging tags are harvested in the physiological mature stage.
Description
Technical field
The invention discloses the quick inbred isolation method of purple capsicum annum fasciculatum in a kind of greenhouse, belong to field of plant variety breeding technology.First selected purple capsicum annum fasciculatum selfing individual plant also carries out listing mark；Next selects, and labelling individual plant is interior is about to open flower；With sticky label paper, flower wrapped and clutch in top and bottom；By this flower listing mark；The fruit of physiological maturity results listing mark.
Background technology
Fructus Capsici belongs to Constantly allogamous plant, and its purification typically requires artificial pollination.There is shortcomings in said method, one is that pollination speed is slow.From rotating and push aside flower, picking pollen, bread at least need 20-30s in stigma, the operation such as list；Two is that pollination efficiency is low.Adopting in 4-5 month and manually pollinate, for Capsicum frutescens L．Var．1o m Bail, Cornu Caprae seu Ovis green pepper, line green pepper, screw green pepper, capsicum annum fasciculatum, thin skin green pepper, its success rate is up to more than 80%, once enter even 6-7 month in 5-6 month, pollination efficiency is substantially reduced；And manually Fructus Capsici is pollinated adopting in 4-5 month, its pollination rate is also only 40-50%；Three is be easily caused biology hybrid.Artificial pollination many employings dissecting needle or syringe needle, as inoculating tool, when different Fructus Capsici selfing linies are inoculated, easily remain the pollen of previous selfing line at instrument leading portion, are easily caused biology hybrid or impure for the pollination of later selfing line.
Summary of the invention
In a kind of greenhouse, the quick inbred isolation method of purple capsicum annum fasciculatum has many superioritys, and one is artificial and consuming time few；Two is that cost is extremely cheap；Three are easy to distinguish selfing or non-selfing fruit.According to above-mentioned cognition and experience, we have invented the quick inbred isolation method of purple capsicum annum fasciculatum in a kind of greenhouse.
The present invention is achieved in that
The quick inbred isolation method of purple capsicum annum fasciculatum in a kind of greenhouse, it is characterized in that, described method includes selected purple capsicum annum fasciculatum selfing individual plant, select flower, parcel flower and clutch, flower listing mark, results five steps such as selfing fruit, reach quickly to obtain the purpose of purple capsicum annum fasciculatum selfing fruit.Five described step concrete operations are as follows:
1. selected individual plant: be selected in and need the purple capsicum annum fasciculatum individual plant of selfing and carry out listing mark；
2. flower is selected: in selected labeled individual plant, be about to open flower, based on the flower to the female bucket of green pepper or four；
3. parcel flower clutching: wrapped by the flower of purple capsicum annum fasciculatum by sticky label paper, is advisable to two circles wrapping up a circle half, and label paper is clutched by the upper and lower side at flower, in case Apis and other insecticide enter；
4. flower listing mark: the bennet place drop at parcel flower carries out listing and dating；
5. results selfing fruit: physiological maturity results bennet has the fruit of drop.
Beneficial effects of the present invention: one is artificial and consuming time few.Early stage has only to buy sticky label paper, without buying dissecting needle or syringe needle, expends 10s every time；Two is that cost is extremely cheap.The cost of sticky label paper is low, and 10 expenses spent are about 5 points of.Three is that labelling is accurate, short and sweet.Unsuccessful if pollinating, flower can drop by nature, and successful flower of pollinating is because bennet place hangs with drop, it is easy to distinguishes.
Detailed description of the invention:
Below in conjunction with specific embodiment, the present invention is conducted further description；
Embodiment:
1) select fine individual plant: in florescence, select excellent purple capsicum annum fasciculatum individual plant according to breeding objective, and hang up red festoons at its place of Shi Huajiewei main split and mould board and be marked；
2) flower is selected: in selected labeled individual plant, be about to open 4 four female bucket flower；
3) wrap up flower and clutch: the sticky label paper of long 2.9cm, wide 2cm being encased flower, wraps up in two circles, and the upper and lower side of label paper is clutched；
4) labelling flower: the drop with the time is suspended on the flower after parcel；
5) results selfing fruit: physiological maturity results hang with the purple capsicum annum fasciculatum fruit 4 of drop, and the time is about 55d to 70 day, and success rate reaches 100%.
Claims (1)
1. the quick inbred isolation method of purple capsicum annum fasciculatum in a greenhouse, it is characterized in that, described method includes selected purple capsicum annum fasciculatum selfing individual plant, select flower, parcel flower and clutch, flower listing mark, results five steps of selfing fruit, reach quickly obtain purple capsicum annum fasciculatum selfing fruit purpose；Five described step concrete operations are as follows:
1. selected individual plant: be selected in and need the purple capsicum annum fasciculatum individual plant of selfing and carry out listing mark；
2. flower is selected: in selected labeled individual plant, be about to open flower, based on the flower to the female bucket of green pepper or four；
3. parcel flower clutching: wrapped by the flower of purple capsicum annum fasciculatum by sticky label paper, is advisable to two circles wrapping up a circle half, and label paper is clutched by the upper and lower side at flower, in case Apis and other insecticide enter；
4. flower listing mark: the bennet place drop at parcel flower carries out listing and dating；
5. results selfing fruit: physiological maturity results bennet has the purple capsicum annum fasciculatum selfing fruit of drop.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610067690.1A CN105706907A (en) | 2016-02-01 | 2016-02-01 | Rapid selfing isolation method for purple pod peppers in greenhouse |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610067690.1A CN105706907A (en) | 2016-02-01 | 2016-02-01 | Rapid selfing isolation method for purple pod peppers in greenhouse |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105706907A true CN105706907A (en) | 2016-06-29 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610067690.1A Pending CN105706907A (en) | 2016-02-01 | 2016-02-01 | Rapid selfing isolation method for purple pod peppers in greenhouse |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105706907A (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1094216A (en) * | 1993-04-28 | 1994-11-02 | 天津市蔬菜研究所 | A kind of method of green pepper hybrid seeding |
| CN102273402A (en) * | 2011-06-30 | 2011-12-14 | 杭州市农业科学研究院 | Pepper inbred isolation method |
| CN103444509A (en) * | 2013-07-16 | 2013-12-18 | 沈素香 | Single flower selfing isolation method for small chili |
-
2016
- 2016-02-01 CN CN201610067690.1A patent/CN105706907A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1094216A (en) * | 1993-04-28 | 1994-11-02 | 天津市蔬菜研究所 | A kind of method of green pepper hybrid seeding |
| CN102273402A (en) * | 2011-06-30 | 2011-12-14 | 杭州市农业科学研究院 | Pepper inbred isolation method |
| CN103444509A (en) * | 2013-07-16 | 2013-12-18 | 沈素香 | Single flower selfing isolation method for small chili |
Non-Patent Citations (4)
| Title |
|---|
| （蔬菜采种技术）编写组: "《蔬菜采种技术》", 31 December 1991 * |
| 刘金兵等: "42个甜(辣)椒自交系恢复基因的分布及亲缘关系的RAPD分析", 《南京农业大学学报》 * |
| 常宏等: "《农作物种子生产技术》", 31 May 2008 * |
| 王述彬等: "辣(甜)椒细胞质雄性不育恢复基因的遗传与分布", 《华北农学报》 * |
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Application publication date: 20160629 |