CN103444509A - Single flower selfing isolation method for small chili - Google Patents
Single flower selfing isolation method for small chili Download PDFInfo
- Publication number
- CN103444509A CN103444509A CN2013102980071A CN201310298007A CN103444509A CN 103444509 A CN103444509 A CN 103444509A CN 2013102980071 A CN2013102980071 A CN 2013102980071A CN 201310298007 A CN201310298007 A CN 201310298007A CN 103444509 A CN103444509 A CN 103444509A
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- petal
- flower
- capsicum
- flower bud
- softgel shell
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Links
- 238000002955 isolation Methods 0.000 title claims abstract description 16
- 235000002568 Capsicum frutescens Nutrition 0.000 title abstract 4
- 239000002775 capsule Substances 0.000 claims abstract description 21
- 238000007726 management method Methods 0.000 claims abstract description 7
- 238000009395 breeding Methods 0.000 claims abstract description 6
- 230000001488 breeding effect Effects 0.000 claims abstract description 6
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 claims abstract description 4
- 241000208293 Capsicum Species 0.000 claims description 21
- 235000002566 Capsicum Nutrition 0.000 claims description 21
- 239000001390 capsicum minimum Substances 0.000 claims description 21
- 241000196324 Embryophyta Species 0.000 claims description 7
- 238000000034 method Methods 0.000 abstract description 25
- 230000010152 pollination Effects 0.000 abstract description 4
- 238000005336 cracking Methods 0.000 abstract description 2
- 238000004519 manufacturing process Methods 0.000 abstract description 2
- 230000035699 permeability Effects 0.000 abstract description 2
- 239000007902 hard capsule Substances 0.000 abstract 1
- 238000003976 plant breeding Methods 0.000 abstract 1
- 229920000742 Cotton Polymers 0.000 description 5
- 239000011324 bead Substances 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 230000005070 ripening Effects 0.000 description 3
- 239000000853 adhesive Substances 0.000 description 2
- 230000001070 adhesive effect Effects 0.000 description 2
- 235000013399 edible fruits Nutrition 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000004744 fabric Substances 0.000 description 2
- 230000035558 fertility Effects 0.000 description 2
- 239000012467 final product Substances 0.000 description 2
- 238000009399 inbreeding Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 239000011505 plaster Substances 0.000 description 2
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 description 1
- 108700028369 Alleles Proteins 0.000 description 1
- 241001164374 Calyx Species 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 108010010803 Gelatin Proteins 0.000 description 1
- 235000008331 Pinus X rigitaeda Nutrition 0.000 description 1
- 235000011613 Pinus brutia Nutrition 0.000 description 1
- 241000018646 Pinus brutia Species 0.000 description 1
- DPXJVFZANSGRMM-UHFFFAOYSA-N acetic acid;2,3,4,5,6-pentahydroxyhexanal;sodium Chemical compound [Na].CC(O)=O.OCC(O)C(O)C(O)C(O)C=O DPXJVFZANSGRMM-UHFFFAOYSA-N 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000001768 carboxy methyl cellulose Substances 0.000 description 1
- -1 cheap Substances 0.000 description 1
- 230000010154 cross-pollination Effects 0.000 description 1
- 239000008367 deionised water Substances 0.000 description 1
- 229910021641 deionized water Inorganic materials 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229920000159 gelatin Polymers 0.000 description 1
- 239000008273 gelatin Substances 0.000 description 1
- 235000019322 gelatine Nutrition 0.000 description 1
- 235000011852 gelatine desserts Nutrition 0.000 description 1
- 230000009931 harmful effect Effects 0.000 description 1
- 239000007943 implant Substances 0.000 description 1
- 238000009413 insulation Methods 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 238000009401 outcrossing Methods 0.000 description 1
- 210000001672 ovary Anatomy 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 230000007198 pollen germination Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 230000010153 self-pollination Effects 0.000 description 1
- 235000010413 sodium alginate Nutrition 0.000 description 1
- 239000000661 sodium alginate Substances 0.000 description 1
- 229940005550 sodium alginate Drugs 0.000 description 1
- 235000019812 sodium carboxymethyl cellulose Nutrition 0.000 description 1
- 229920001027 sodium carboxymethylcellulose Polymers 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Landscapes
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention belongs to the technical field of plant breeding, and in particular relates to a single flower selfing isolation method for small chili. The method comprises the following steps: (1) selecting an excellent single plant, namely in the flowering period, selecting the excellent single plant according to a breeding objective, and marking; (2) preparing a capsule shell, namely separating a shell cap from a shell body of a hollow hard capsule shell; (3) selecting a flower bud, namely selecting a large flower bud which is expected to bloom in the next day; (4) treating the flower bud, namely holding the flower bud to be treated by one hand, holding the capsule shell, which is matched with the flower bud in size, by the other hand, slightly sleeving the flower bud with the lower opening of the capsule shell over the widest part of the flower bud, and marking a flower stalk; (5) managing after the treatment in a method which is the same as a conventional field management method for the production of small chili. The capsule shell used in the method is low in cost and does not hurt the flower bud and the flower stalk when being used, the operation is convenient and easy, and the work efficiency is high; moreover, the capsule shell with certain elasticity and permeability does not influence the cracking of anther and pollination, and thus the selfing setting rate of the small chili is high.
Description
Technical field
The invention belongs to field of plant variety breeding technology, be specifically related to a kind of single flower inbred isolation method of capsicum.
Background technology
Capsicum is generally first to bloom to split afterwards medicine, and every flower lasts about 3d by opening into to wither.The stamen pollen vitality is the highest with the same day of blooming, and the front 1d of blooming takes second place, and bloom front 2d and the rear 1d of blooming are the most weak, and its active pollen ratio is respectively 85%, 58%, 21.3% and 19%.Gynoecium 2d before blooming is the tool fertility, but the strongest with the same day of blooming, and the front 1d of blooming takes second place.The pollination fruit-setting rate of bloom front 2d, blooming front 1d and the same day of blooming is respectively 21.4%, 57.1%, 71.4%, and the rear 2-3d gynoecium fertility of blooming obviously weakens or loses.After self-pollination, 2-4h pollinia on column cap is sprouted, and the 8-12h pollen tube enters ovary, and the 12-20h pollen tube enters in blastular and discharges sperm through the hole of bead, and the female male cell of 14-30h merges, the nuclear division of 18-40h primary endosperm, the division of 4d left and right zygote.Evidence, after first pollen tube discharges pollen through the hole of bead in blastular, the hole of bead is closed, to stop entering of other pollen tubes.Pollen germination be take 20-26 ℃ as best, fruit-setting rate during with relative moisture 55-75% for the highest.When daily mean temperature, higher than 30 ℃, relative air humidity, lower than 40% or when above higher than 90%, causes harmful effect to result.
In capsicum breed breeding process, the material isozygotied in order to obtain allelomorph, must allow capsicum carry out the selfing column cap of own gynoecium (the same pollen in spending award).But capsicum is a kind of Chang Yihua pollination crop, its natural outcrossing 20%.In order to guarantee selfing, must, in the pedestrian's work isolation of advancing of blooming, prevent cross pollination.According to the characteristic of growing of aforementioned capsicum, isolation time is advisable to the rear 2d of blooming with the front 1d of blooming.The method of capsicum list flower selfing isolation has the bagging method; Tiretube process, twine cotton method (" comparisons of three kinds of autocopulation methods of capsicum " Shen Suxiang etc., " seed " the 3rd phase in 2006); Adhesive plaster twines subsides method (Liu Jinbing etc., 2009) and solution film forming method (Chai Weiguo etc., 201110181629.7) etc.
The bagging method is to select the large bud of blooming tomorrow or the day after tomorrow, and the paper bag of that make with parchment, long and wide all about 3cm entangles, paper bag base portion knuckle, then clamp with clip.The shortcoming of this method, the one, do paper bag time-consuming; The 2nd, during operation, tender petal and anthocaulus easily is bruised and fractures.
Tiretube process is that the plastic suction pipe of appropriate diameter is cut into to 2cm length, and an end slightly pinches sealing after softened by baking with the alcohol lights, during selfing, large petal is entangled and gets final product.The shortcoming of this method is that plastic tube is waterproof, airtight, there is no elasticity, and after the petal quilt cover, petal can't be upheld, and inner moisture is large, and ripening rate is low.
Twining the cotton method is to tear to get a fritter absorbent cotton, and cotton fiber is pulled open, and is wrapped on petal, and bud is snarled.Its shortcoming is higher to twining colored specification requirement, too pine cotton easily fall maybe to hold onto petal, the too tight growth that can affect its later fruit.
It is to use medical proof fabric that the capsicum petal is wrapped that adhesive plaster twines the subsides method.Shortcoming is time-consuming while wrapping up in petal, and efficiency is lower; Petal is also excessively fettered simultaneously.
The solution film forming method is to be the blend solution that 5-10%, sodium carboxymethylcellulose mass concentration are 0.5-2% by deionized water preparation sodium alginate mass concentration, petal is immersed in blend solution, until the junction of calyx and anthocaulus.The shortcoming of this method, the one, obtain solution bothers; The 2nd, petal to be processed is to estimate the large petal of opening after 6-12h, and the time that capsicum blooms mostly is point in the mornings 6-11, therefore needs between the lights or soak the flower processing with solution at night, and operation is inconvenience very.
Summary of the invention
The single flower inbred isolation method that the purpose of this invention is to provide a kind of with low cost, environmentally friendly, capsicum that effect is good.
For achieving the above object, the invention provides a kind of single flower inbred isolation method of capsicum, comprise the following steps:
1) select fine individual plant: in flowering stage, according to breeding objective, select fine individual plant, and mark;
2) capsule shells is prepared: the shell cap of empty hard softgel shell and body are separated;
3) petal is selected: selecting to estimate can be at open large petal next day;
4) petal is processed: pending petal is taken in a have gentle hands, and another hand is taken the capsule shells (shell cap or body) matched with the petal size, lightly petal is entangled, and makes the capsule shells end opening cross petal the widest part, and carries out mark at the anthocaulus place;
5) management after processing: with capsicum, to produce conventional field management method identical.
The size of described empty hard softgel shell can be determined according to the size of petal, can be that the hard-hearted capsule shells of standard null that directly buys on market is as 0#, 1#, 2# empty hard softgel shell, also can customize as producer the empty hard softgel shell of specific size, preferably, body oral area external diameter between 6.0-7.4mm, body length is between 15.0-18.5mm;
Preferred, described empty hard softgel shell is enteric solubility empty hard softgel shell, and to prevent meeting with rainwater when the outdoor implant mass, enteric solubility empty hard softgel shell solvability in slightly acidic rainwater is lower, can better guarantee isolation effect.
Described empty hard softgel shell can be shades of colour, and chromatic colour is conducive to the empty hard softgel shell of identification and the mark, particularly bright yellow of cuff selfing flower, can be easy to distinguish with the flower of field white, the leaf of green and red fruit.
Shell cap or body all can be used for overlapping petal, as long as under the prerequisite of not damaging petal, the capsule shells end opening can be crossed petal the widest part and get final product.
Beneficial effect of the present invention is as follows:
(1) cover petal selfing insulation rubber softgel shell used can be entrusted the manufacturer production of producing Capsules, cheap, and capsule shells is mainly gelatin, can be degradable at occurring in nature, and more friendly to environment;
(2) because material is light and handy, do not injure bud and bennet during use, easy to operation, high efficiency;
(3) capsule shells has certain elasticity and gas permeability, does not affect cracking and the pollination of flower pesticide, and self-fruitful rate is high.
Embodiment
Below in conjunction with embodiment, the invention will be further described.
Embodiment
1) select fine individual plant: in flowering stage, select fine individual plant according to breeding objective, and make marks by pink plastic ties;
2) capsule shells is prepared: the shell cap of the empty hard softgel shell of the 0# that will buy in advance, 1# and the bright yellow of 2# and body are separately, and separated according to size, in order to use according to petal size fast fetching during operation;
3) petal is selected: selecting to estimate can be at open large petal next day;
4) petal is processed: pending petal is taken in a have gentle hands, and another hand is taken the capsule shells matched with the petal size, lightly petal is entangled, and makes the capsule shells end opening cross petal the widest part, and carries out mark with marker pen at the anthocaulus place;
5) management after processing: with capsicum, to produce conventional field management method identical.
6) treatment effect: with 6 flowers of cover capsule shells method average inbreeding isolation per minute, ripening rate is 66.7%, and isolate 3,2,4 with touching colored method, sticking cloth method, solution film forming method average inbreeding respectively per minute, ripening rate is respectively 63%, 53.3%, 57.7%.Visible, the work efficiency of cover capsule shells method is apparently higher than other several method.
All documents that the present invention mentions are all quoted as a reference in this application, just as each piece of document quoted separately as a reference.
Should be appreciated that above narration is only example and illustrative for the present invention who is set forth as claim, is not limited it.The disclosed content of the present invention according to this paper, other embodiment of the present invention is apparent for a person skilled in the art.Should be noted that this specification and embodiment only should be regarded as example, actual range of the present invention is determined by claim.
Claims (5)
1. a capsicum list is spent inbred isolation method, comprises the following steps:
1) select fine individual plant: in flowering stage, according to breeding objective, select fine individual plant, and mark;
2) capsule shells is prepared: the shell cap of empty hard softgel shell and body are separated;
3) petal is selected: selecting to estimate can be at open large petal next day;
4) petal is processed: pending petal is taken in a have gentle hands, and another hand is taken the capsule shells matched with the petal size, lightly petal is entangled, and makes the capsule shells end opening cross petal the widest part, and carries out mark at the anthocaulus place;
5) management after processing: with capsicum, to produce conventional field management method identical.
2. capsicum list flower inbred isolation method as claimed in claim 1, is characterized in that the diameter of described empty hard softgel shell is between 0.63-0.77mm, highly between 15-18.5mm.
3. capsicum list flower inbred isolation method as claimed in claim 1, is characterized in that described empty hard softgel shell is 0#, 1# and 2# empty hard softgel shell.
4. capsicum list flower inbred isolation method as described as claim 1-3 any one, is characterized in that described empty hard softgel shell is enteric solubility empty hard softgel shell.
5. capsicum list flower inbred isolation method as described as claim 1-3 any one, is characterized in that described empty hard softgel shell is bright yellow.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310298007.1A CN103444509B (en) | 2013-07-16 | 2013-07-16 | A kind of list flower inbred isolation method of capsicum |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310298007.1A CN103444509B (en) | 2013-07-16 | 2013-07-16 | A kind of list flower inbred isolation method of capsicum |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103444509A true CN103444509A (en) | 2013-12-18 |
| CN103444509B CN103444509B (en) | 2016-04-13 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310298007.1A Expired - Fee Related CN103444509B (en) | 2013-07-16 | 2013-07-16 | A kind of list flower inbred isolation method of capsicum |
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Cited By (39)
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| CN104082130A (en) * | 2014-07-30 | 2014-10-08 | 胡能兵 | Method for quickly positioning selfing fruit of pepper |
| CN104115742A (en) * | 2014-07-30 | 2014-10-29 | 胡能兵 | Thread-guiding-type pepper selfing fruit marking method |
| CN104126500A (en) * | 2014-07-30 | 2014-11-05 | 胡能兵 | Lead marker and chemical enclosing combined method for positioning inbred fruit of capsicum |
| CN104137770A (en) * | 2014-07-30 | 2014-11-12 | 胡能兵 | Method for quickly marking fruits obtained through selfing of peppers |
| CN104823831A (en) * | 2015-04-13 | 2015-08-12 | 新疆农垦科学院 | Sunflower inbred line single flower isolation breeding method |
| CN105432460A (en) * | 2016-02-01 | 2016-03-30 | 钱夕刚 | Thread double-knot type deep yellow skin screw pepper selfing technique |
| CN105454035A (en) * | 2016-02-01 | 2016-04-06 | 钱夕刚 | Fine thread double-knot type purple thin cayenne pepper selfing technology |
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| CN105475124A (en) * | 2016-02-01 | 2016-04-13 | 何其家 | Rapid selfing isolation method for red-skin capsicum annuum in greenhouse |
| CN105532439A (en) * | 2016-02-01 | 2016-05-04 | 钱夕华 | Rapid selfing isolation method for purple thin-skinned peppers in greenhouse |
| CN105532438A (en) * | 2016-02-01 | 2016-05-04 | 钱夕华 | Fine line loop knot type deep yellow thin-skinned pepper selfing technology |
| CN105532440A (en) * | 2016-02-01 | 2016-05-04 | 钱夕华 | Rapid selfing isolation method for cream yellow thin-skinned peppers in greenhouse |
| CN105613263A (en) * | 2016-02-01 | 2016-06-01 | 钱夕刚 | Thread loop-knot type deep yellow Capsicum annuum L. var. conoides (Mill.) Irish selfing technology |
| CN105613274A (en) * | 2016-02-01 | 2016-06-01 | 何其家 | Rapid selfing isolation method for deep yellow sweet peppers in greenhouses |
| CN105613268A (en) * | 2016-02-01 | 2016-06-01 | 钱夕华 | Thread loop-knot type cream yellow sheep-horn pepper selfing technology |
| CN105613273A (en) * | 2016-02-01 | 2016-06-01 | 何其家 | Rapid selfing isolation method for purple sweet peppers in greenhouses |
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| CN105613269A (en) * | 2016-02-01 | 2016-06-01 | 钱夕华 | Rapid selfing isolation method for deep yellow cow-horn peppers in greenhouses |
| CN105638444A (en) * | 2016-02-01 | 2016-06-08 | 钱夕华 | Dark yellow thin-peel pepper quick selfing isolation method used in greenhouse |
| CN105638445A (en) * | 2016-02-01 | 2016-06-08 | 何其家 | Cream yellow bell pepper quick selfing isolation method used in greenhouse |
| CN105638439A (en) * | 2016-02-01 | 2016-06-08 | 李吉菊 | Purple Hangzhou pepper quick selfing isolation method used in greenhouse |
| CN105638442A (en) * | 2016-02-01 | 2016-06-08 | 钱夕华 | Red-peel ox horn pepper quick selfing isolation method used in greenhouse |
| CN105660368A (en) * | 2016-02-01 | 2016-06-15 | 钱夕刚 | Thread dual-knot type selfing technology for red sweet pepper |
| CN105660369A (en) * | 2016-02-01 | 2016-06-15 | 钱夕华 | Thread dual-knot type selfing technology for red sheep-horn pepper |
| CN105660374A (en) * | 2016-02-01 | 2016-06-15 | 何其家 | Method for rapid selfing isolation of red bell pepper in greenhouse |
| CN105660372A (en) * | 2016-02-01 | 2016-06-15 | 何其家 | Method for rapid selfing isolation of dark-yellow pod pepper in greenhouse |
| CN105660373A (en) * | 2016-02-01 | 2016-06-15 | 何其家 | Method for rapid selfing isolation of dark-yellow bell pepper in greenhouse |
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| CN105684903A (en) * | 2016-02-01 | 2016-06-22 | 李吉菊 | Inbreeding technique of filament double-knot type dark yellow skin cow-horn pepper |
| CN105706907A (en) * | 2016-02-01 | 2016-06-29 | 何其家 | Rapid selfing isolation method for purple pod peppers in greenhouse |
| CN105706908A (en) * | 2016-02-01 | 2016-06-29 | 何其家 | Rapid selfing isolation method for purple screw peppers in a greenhouse, |
| CN105706905A (en) * | 2016-02-01 | 2016-06-29 | 李吉菊 | Rapid selfing isolation method for deep yellow Hangzhou peppers in greenhouse |
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| CN105638442A (en) * | 2016-02-01 | 2016-06-08 | 钱夕华 | Red-peel ox horn pepper quick selfing isolation method used in greenhouse |
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| CN105475124A (en) * | 2016-02-01 | 2016-04-13 | 何其家 | Rapid selfing isolation method for red-skin capsicum annuum in greenhouse |
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| CN105532438A (en) * | 2016-02-01 | 2016-05-04 | 钱夕华 | Fine line loop knot type deep yellow thin-skinned pepper selfing technology |
| CN105532440A (en) * | 2016-02-01 | 2016-05-04 | 钱夕华 | Rapid selfing isolation method for cream yellow thin-skinned peppers in greenhouse |
| CN105613263A (en) * | 2016-02-01 | 2016-06-01 | 钱夕刚 | Thread loop-knot type deep yellow Capsicum annuum L. var. conoides (Mill.) Irish selfing technology |
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| CN105684903A (en) * | 2016-02-01 | 2016-06-22 | 李吉菊 | Inbreeding technique of filament double-knot type dark yellow skin cow-horn pepper |
| CN105706907A (en) * | 2016-02-01 | 2016-06-29 | 何其家 | Rapid selfing isolation method for purple pod peppers in greenhouse |
| CN105706908A (en) * | 2016-02-01 | 2016-06-29 | 何其家 | Rapid selfing isolation method for purple screw peppers in a greenhouse, |
| CN105706905A (en) * | 2016-02-01 | 2016-06-29 | 李吉菊 | Rapid selfing isolation method for deep yellow Hangzhou peppers in greenhouse |
| CN109315392A (en) * | 2018-10-10 | 2019-02-12 | 江苏沿江地区农业科学研究所 | Suitable for the mating and preparation method thereof of solanaceous vegetables selfing |
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