CN105532439A - Rapid selfing isolation method for purple thin-skinned peppers in greenhouse - Google Patents

Abstract

The invention discloses a rapid selfing isolation method for purple thin-skinned peppers in a greenhouse, and belongs to the technical field of plant breeding. The method comprises the following steps of selecting a single purple thin-skinned pepper plant to be selfed, and performing labeling; then selecting flowers about to come out in the labeled single plant; wrapping the flowers with sticky label paper, and tightening the upper and lower ends of the sticky label paper; labeling the flowers; harvesting labeled fruits in a physiological mature stage.

Description

The quick inbred isolation method of purple thin skin green pepper in a kind of greenhouse

Technical field

The invention discloses the quick inbred isolation method of purple thin skin green pepper in a kind of greenhouse, belong to field of plant variety breeding technology.First selected purple thin skin green pepper selfing individual plant also carries out listing mark; Secondly select to have marked in individual plant to be about to open flower; With sticky label paper flower wrapped and clutch in top and bottom; By this flower listing mark; The fruit of physiological maturity results listing mark.

Background technology

Capsicum belongs to Constantly allogamous plant, and its purifying needs artificial pollination usually.There is shortcomings in said method, one is that pollination speed is slow.From rotation and push flower aside, picking pollen, bread at least need 20-30s in column cap, the operation such as to list; Two is that pollination efficiency is low.Adopt in 4-5 month and manually pollinate, for long red pepper, goat's horn green pepper, line green pepper, screw green pepper, capsicum annum fasciculatum, thin skin green pepper, its success rate can reach more than 80%, once enter even 6-7 month in 5-6 month, pollination efficiency reduces greatly; And manually pimento is pollinated adopting in 4-5 month, its pollination rate is also only 40-50%; Three is easily cause biology hybrid.Artificial pollination many employings dissecting needle or syringe needle, as inoculating tool, when inoculating different capsicum inbred line, easily remain the pollen of previous inbred line at instrument leading portion, easily cause biology hybrid or impure for rear inbred line pollination.

Summary of the invention

In greenhouse, the quick inbred isolation method of purple thin skin green pepper has many superiority, and one is artificial and consuming time few; Two is that cost is extremely cheap; Three is be easy to distinguish selfing or non-selfing fruit.According to above-mentioned cognition and experience, we have invented the quick inbred isolation method of purple thin skin green pepper in a kind of greenhouse.

The present invention is achieved in that

The quick inbred isolation method of purple thin skin green pepper in a kind of greenhouse, it is characterized in that, include selected purple thin skin green pepper selfing individual plant in described method, select flower, parcel flower and clutch, the five steps such as flower listing mark, results selfing fruit, reach the object obtaining purple thin skin green pepper selfing fruit fast.Described five steps concrete operations are as follows:

1. selected individual plant: be selected in and need the purple thin skin green pepper individual plant of selfing and carry out listing mark;

2. flower is selected: in the selected individual plant marked, be about to open flower, based on the flower to green pepper or four female buckets;

3. wrap up flower and clutch: the flower of sticky label paper by purple thin skin green pepper is wrapped, being advisable to two circles to wrap up a circle half, at the upper and lower side of flower, label paper being clutched, in case honeybee and other insect enter;

4. flower listing mark: carry out listing and dating at the bennet place drop of parcel flower;

5. selfing fruit is gathered in the crops: physiological maturity results bennet has the fruit of drop.

Beneficial effect of the present invention: one is artificial and consuming time few.Only need early stage to buy sticky label paper, and without the need to buying dissecting needle or syringe needle, expend 10s at every turn; Two is that cost is extremely cheap.The cost of sticky label paper is low, and 10 expenses spent are about 5 points of.Three is that mark is accurate, short and sweet.Unsuccessful if pollinate, flower can drop by nature, and successful flower of pollinating is because bennet place hangs with drop, easily distinguishes.

embodiment:

Below in conjunction with specific embodiment, the present invention is conducted further description;

Embodiment:

1) select fine individual plant: in flowering stage, select excellent purple thin skin green pepper individual plant according to breeding objective, and hang up red festoons at its place of Shi Huajiewei main split and mould board and marked;

2) flower is selected: in the selected individual plant marked, be about to 4 four open female bucket flowers;

3) wrap up flower and clutch: the sticky label paper of long 2.9cm, wide 2cm being encased flower, wraps up in two circles, and the upper and lower side of label paper is clutched;

4) flower is marked: be suspended to by the drop with the time on the flower after parcel;

5) selfing fruit is gathered in the crops: physiological maturity results hang with the purple thin skin green pepper fruit 4 of drop, and the time is about 55d to 70 day, and success rate reaches 100%.

Claims (1)

1. the quick inbred isolation method of purple thin skin green pepper in a greenhouse, it is characterized in that, include selected purple thin skin green pepper selfing individual plant in described method, select flower, parcel flower and clutch, flower listing mark, results selfing fruit five steps, reach the object obtaining purple thin skin green pepper selfing fruit fast; Described five steps concrete operations are as follows:

1. selected individual plant: be selected in and need the purple thin skin green pepper individual plant of selfing and carry out listing mark;

2. flower is selected: in the selected individual plant marked, be about to open flower, based on the flower to green pepper or four female buckets;

3. wrap up flower and clutch: the flower of sticky label paper by purple thin skin green pepper is wrapped, being advisable to two circles to wrap up a circle half, at the upper and lower side of flower, label paper being clutched, in case honeybee and other insect enter;

4. flower listing mark: carry out listing and dating at the bennet place drop of parcel flower;

5. selfing fruit is gathered in the crops: physiological maturity results bennet has the purple thin skin green pepper selfing fruit of drop.