CN105613270A - Rapid selfing isolation method for cream yellow cow-horn peppers in greenhouses - Google Patents

Rapid selfing isolation method for cream yellow cow-horn peppers in greenhouses Download PDF

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Publication number
CN105613270A
CN105613270A CN201610067680.8A CN201610067680A CN105613270A CN 105613270 A CN105613270 A CN 105613270A CN 201610067680 A CN201610067680 A CN 201610067680A CN 105613270 A CN105613270 A CN 105613270A
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China
Prior art keywords
flower
bail
var
selfing
capsicum frutescens
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CN201610067680.8A
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Chinese (zh)
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钱夕华
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Individual
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Individual
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Priority to CN201610067680.8A priority Critical patent/CN105613270A/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/02Methods or apparatus for hybridisation; Artificial pollination ; Fertility

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

The invention discloses a rapid selfing isolation method for cream yellow cow-horn peppers in greenhouses and belongs to the technical field of plant breeding. The rapid selfing isolation method includes: firstly, selecting single selfing plants of the cream yellow cow-horn peppers prior to labeling; secondly, selecting flowers about to bloom from the labeled single plants; thirdly, wrapping every flower with sticky label paper, and pinching the upper end and the lower end of the sticky label paper; fourthly, labeling every flower; fifthly, harvesting labeled fruit in a physiological mature stage.

Description

The quick inbred isolation method of milk yellow Capsicum frutescens L��Var��1o m Bail in a kind of greenhouse
Technical field
The invention discloses the quick inbred isolation method of milk yellow Capsicum frutescens L��Var��1o m Bail in a kind of greenhouse, belong to field of plant variety breeding technology. First selected milk yellow Capsicum frutescens L��Var��1o m Bail selfing individual plant also carries out listing mark; Next selects, and labelling individual plant is interior is about to open flower; With sticky label paper, flower wrapped and clutch in top and bottom; By this flower listing mark; The fruit of physiological maturity results listing mark.
Background technology
Fructus Capsici belongs to Constantly allogamous plant, and its purification typically requires artificial pollination. There is shortcomings in said method, one is that pollination speed is slow. From rotating and push aside flower, picking pollen, bread at least need 20-30s in stigma, the operation such as list; Two is that pollination efficiency is low. Adopting in 4-5 month and manually pollinate, for Capsicum frutescens L��Var��1o m Bail, Cornu Caprae seu Ovis green pepper, line green pepper, screw green pepper, capsicum annum fasciculatum, thin skin green pepper, its success rate is up to more than 80%, once enter even 6-7 month in 5-6 month, pollination efficiency is substantially reduced; And manually Fructus Capsici is pollinated adopting in 4-5 month, its pollination rate is also only 40-50%; Three is be easily caused biology hybrid. Artificial pollination many employings dissecting needle or syringe needle, as inoculating tool, when different Fructus Capsici selfing linies are inoculated, easily remain the pollen of previous selfing line at instrument leading portion, are easily caused biology hybrid or impure for the pollination of later selfing line.
Summary of the invention
In a kind of greenhouse, the quick inbred isolation method of milk yellow Capsicum frutescens L��Var��1o m Bail has many superioritys, and one is artificial and consuming time few; Two is that cost is extremely cheap; Three are easy to distinguish selfing or non-selfing fruit. According to above-mentioned cognition and experience, we have invented the quick inbred isolation method of milk yellow Capsicum frutescens L��Var��1o m Bail in a kind of greenhouse.
The present invention is achieved in that
The quick inbred isolation method of milk yellow Capsicum frutescens L��Var��1o m Bail in a kind of greenhouse, it is characterized in that, described method includes selected milk yellow Capsicum frutescens L��Var��1o m Bail selfing individual plant, select flower, parcel flower and clutch, flower listing mark, results five steps such as selfing fruit, reach quickly to obtain the purpose of milk yellow Capsicum frutescens L��Var��1o m Bail selfing fruit. Five described step concrete operations are as follows:
1. selected individual plant: be selected in and need the milk yellow Capsicum frutescens L��Var��1o m Bail individual plant of selfing and carry out listing mark;
2. flower is selected: in selected labeled individual plant, be about to open flower, based on the flower to the female bucket of green pepper or four;
3. parcel flower clutching: wrapped by the flower of milk yellow Capsicum frutescens L��Var��1o m Bail by sticky label paper, is advisable to two circles wrapping up a circle half, and label paper is clutched by the upper and lower side at flower, in case Apis and other insecticide enter;
4. flower listing mark: the bennet place drop at parcel flower carries out listing and dating;
5. results selfing fruit: physiological maturity results bennet has the fruit of drop.
Beneficial effects of the present invention: one is artificial and consuming time few. Early stage has only to buy sticky label paper, without buying dissecting needle or syringe needle, expends 10s every time; Two is that cost is extremely cheap. The cost of sticky label paper is low, and 10 expenses spent are about 5 points of. Three is that labelling is accurate, short and sweet. Unsuccessful if pollinating, flower can drop by nature, and successful flower of pollinating is because bennet place hangs with drop, it is easy to distinguishes.
Detailed description of the invention:
Below in conjunction with specific embodiment, the present invention is conducted further description;
Embodiment:
1) select fine individual plant: in florescence, select excellent milk yellow Capsicum frutescens L��Var��1o m Bail individual plant according to breeding objective, and hang up red festoons at its place of Shi Huajiewei main split and mould board and be marked;
2) flower is selected: in selected labeled individual plant, be about to open 4 four female bucket flower;
3) wrap up flower and clutch: the sticky label paper of long 2.9cm, wide 2cm being encased flower, wraps up in two circles, and the upper and lower side of label paper is clutched;
4) labelling flower: the drop with the time is suspended on the flower after parcel;
5) results selfing fruit: physiological maturity results hang with the milk yellow Capsicum frutescens L��Var��1o m Bail fruit 4 of drop, and the time is about 55d to 70 day, and success rate reaches 100%.

Claims (1)

1. the quick inbred isolation method of milk yellow Capsicum frutescens L��Var��1o m Bail in a greenhouse, it is characterized in that, described method includes selected milk yellow Capsicum frutescens L��Var��1o m Bail selfing individual plant, select flower, parcel flower and clutch, flower listing mark, results five steps of selfing fruit, reach quickly obtain milk yellow Capsicum frutescens L��Var��1o m Bail selfing fruit purpose; Five described step concrete operations are as follows:
1. selected individual plant: be selected in and need the milk yellow Capsicum frutescens L��Var��1o m Bail individual plant of selfing and carry out listing mark;
2. flower is selected: in selected labeled individual plant, be about to open flower, based on the flower to the female bucket of green pepper or four;
3. parcel flower clutching: wrapped by the flower of milk yellow Capsicum frutescens L��Var��1o m Bail by sticky label paper, is advisable to two circles wrapping up a circle half, and label paper is clutched by the upper and lower side at flower, in case Apis and other insecticide enter;
4. flower listing mark: the bennet place drop at parcel flower carries out listing and dating;
5. results selfing fruit: physiological maturity results bennet has the milk yellow Capsicum frutescens L��Var��1o m Bail selfing fruit of drop.
CN201610067680.8A 2016-02-01 2016-02-01 Rapid selfing isolation method for cream yellow cow-horn peppers in greenhouses Pending CN105613270A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201610067680.8A CN105613270A (en) 2016-02-01 2016-02-01 Rapid selfing isolation method for cream yellow cow-horn peppers in greenhouses

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201610067680.8A CN105613270A (en) 2016-02-01 2016-02-01 Rapid selfing isolation method for cream yellow cow-horn peppers in greenhouses

Publications (1)

Publication Number Publication Date
CN105613270A true CN105613270A (en) 2016-06-01

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
CN201610067680.8A Pending CN105613270A (en) 2016-02-01 2016-02-01 Rapid selfing isolation method for cream yellow cow-horn peppers in greenhouses

Country Status (1)

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CN (1) CN105613270A (en)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104082130A (en) * 2014-07-30 2014-10-08 胡能兵 Method for quickly positioning selfing fruit of pepper
CN104115742A (en) * 2014-07-30 2014-10-29 胡能兵 Thread-guiding-type pepper selfing fruit marking method

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104082130A (en) * 2014-07-30 2014-10-08 胡能兵 Method for quickly positioning selfing fruit of pepper
CN104115742A (en) * 2014-07-30 2014-10-29 胡能兵 Thread-guiding-type pepper selfing fruit marking method

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
戈加欣等: "双绿5号蚕豆单花强制自交提纯技术研究", 《种子生产》 *

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