CN103444509B - A kind of list flower inbred isolation method of capsicum - Google Patents
A kind of list flower inbred isolation method of capsicum Download PDFInfo
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- CN103444509B CN103444509B CN201310298007.1A CN201310298007A CN103444509B CN 103444509 B CN103444509 B CN 103444509B CN 201310298007 A CN201310298007 A CN 201310298007A CN 103444509 B CN103444509 B CN 103444509B
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Abstract
The invention belongs to field of plant variety breeding technology, be specifically related to a kind of list flower inbred isolation method of capsicum, comprise the following steps: 1) select fine individual plant: in flowering stage, select fine individual plant according to breeding objective, and mark; 2) capsule shells prepares: the shell cap of empty hard softgel shell and body are separated; 3) petal is selected: select the large petal estimating to open in next day; 4) petal process: pending petal is taken in a have gentle hands, and another hand-held capsule shells matched with petal size, entangles petal lightly, makes capsule shells end opening cross petal the widest part, and carries out mark at anthocaulus place; 5) management after process: to produce conventional field management method identical with capsicum.The present invention's capsule shells used is with low cost, and do not injure bud and bennet during use, easy to operation, operating efficiency is high; And capsule shells has certain elasticity and gas permeability, do not affect cracking and the pollination of flower pesticide, self-fruitful rate is high.
Description
Technical field
The invention belongs to field of plant variety breeding technology, be specifically related to a kind of list flower inbred isolation method of capsicum.
Background technology
Capsicum is generally that first Post flowering splits medicine, and every flower lasts about 3d by opening into wither.Stamen pollen vitality is the highest with the same day of blooming, and before blooming, 1d takes second place, and before blooming, 2d and Post flowering 1d is the most weak, and its active pollen ratio is respectively 85%, 58%, 21.3% and 19%.Gynoecium is 2d and tool fertility before flowering, but the strongest with the same day of blooming, and before blooming, 1d takes second place.2d before blooming, bloom before 1d and the pollination fruit-setting rate on the same day of blooming be respectively 21.4%, 57.1%, 71.4%, Post flowering 2-3d gynoecium fertility obviously weakens or loses.After self-pollination, 2-4h pollinia on column cap is sprouted, and 8-12h pollen tube enters ovary, and 12-20h pollen tube enters release sperm in blastular through the hole of bead, and the female male cell of 14-30h merges, the nuclear division of 18-40h primary endosperm, and about 4d zygote divides.Test proves, after first pollen tube discharges pollen through the hole of bead in blastular, namely the hole of bead is closed, to stop entering of other pollen tubes.Pollen germination is best with 20-26 DEG C, and fruit-setting rate is being the highest during relative moisture 55-75%.When daily mean temperature is higher than 30 DEG C, relative air humidity lower than 40% or higher than more than 90% time, harmful effect is caused to result.
In capsicum breed breeding process, in order to obtain the material that allelomorph isozygotys, must allow capsicum carry out selfing (same spend in pollen award the column cap of oneself gynoecium).But capsicum is a kind of Chang Yihua pollination crop, its natural outcrossing 20%.In order to ensure selfing, manually must isolate before flowering, preventing cross pollination.According to the characteristic of growing of aforementioned capsicum, isolation time is advisable to Post flowering 2d with 1d before blooming.The method of capsicum list flower selfing isolation has bagging method; Tiretube process, twine cotton method (" comparisons of capsicum three kinds of autocopulation methods " Shen Suxiang etc., " seed " the 3rd phase in 2006); Adhesive plaster twines subsides method (Liu Jinbing etc., 2009) and solution film formation (Chai Weiguo etc., 201110181629.7) etc.
Bagging method is the large bud selecting to bloom tomorrow or the day after tomorrow, and the paper bag of that make with parchment, long and wide all about 3cm entangles, paper bag base portion knuckle, then clamps with clip.The shortcoming of this method, one is that to do paper bag time-consuming; Two tender petals when being operations and anthocaulus are easily bruised and fracture.
Tiretube process is that the plastic suction pipe of appropriate diameter to be cut into 2cm long, and one end alcohol lights slightly pinch sealing after softened by baking, are entangled by large petal during selfing.The shortcoming of this method is that plastic tube is waterproof, airtight, and do not have elasticity, after petal quilt cover, petal cannot be upheld, and inner moisture is large, and ripening rate is low.
Twining cotton method is tear to get a fritter absorbent cotton, is pulled open by cotton fiber, is wrapped on petal, is snarled by bud.Its shortcoming is higher to twining colored technical requirement, and too pine cotton easily falls and maybe can not hold onto petal, the too tight growth that can affect fruit after it.
It is use medical proof fabric to be wrapped by capsicum petal that adhesive plaster twines subsides method.Shortcoming is time-consuming when wrapping up in petal, and efficiency is lower; Petal is also excessively fettered simultaneously.
Solution film formation be with deionized water preparation sodium alginate mass concentration be 5-10%, sodium carboxymethylcellulose mass concentration is the blend solution of 0.5-2%, petal is immersed in blend solution, until the junction of calyx and anthocaulus.The shortcoming of this method, one is that obtain solution comparatively bothers; Two is petals to be processed is estimate large petal open after 6-12h, and capsicum opens the time spent and mostly is point in the morning 6-11, and therefore need between the lights or the process of solution leaching night flower, operation is very inconvenient.
Summary of the invention
The object of this invention is to provide a kind of list flower inbred isolation method of with low cost, environmentally friendly, capsicum that effect is good.
For achieving the above object, the invention provides a kind of list flower inbred isolation method of capsicum, comprise the following steps:
1) select fine individual plant: in flowering stage, select fine individual plant according to breeding objective, and mark;
2) capsule shells prepares: the shell cap of empty hard softgel shell and body are separated;
3) petal is selected: select the large petal estimating to open in next day;
4) petal process: pending petal is taken in a have gentle hands, and another hand-held capsule shells (shell cap or body) matched with petal size, entangles petal lightly, make capsule shells end opening cross petal the widest part, and carry out mark at anthocaulus place;
5) management after process: to produce conventional field management method identical with capsicum.
The size of described empty hard softgel shell can be determined according to the size of petal, can be that the hard-hearted capsule shells of standard null that market directly buys is as 0#, 1#, 2# empty hard softgel shell, also the empty hard softgel shell of specific size can be customized as producer, preferably, body oral area external diameter between 6.0-7.4mm, body length is between 15.0-18.5mm;
Preferred, described empty hard softgel shell is enteric solubility empty hard softgel shell, and to prevent from meeting with rainwater when outdoor implant mass, enteric solubility empty hard softgel shell solvability in slightly acidic rainwater is lower, better can ensure isolation effect.
Described empty hard softgel shell can be shades of colour, and chromatic colour is conducive to the empty hard softgel shell of identification and the mark, particularly bright yellow of cuff selfing flower, can be easy to distinguish with the fruit of the flower of field white, green leaf and redness.
Shell cap or body all can be used for overlapping petal, as long as under the prerequisite not damaging petal, capsule shells end opening can cross petal the widest part.
Beneficial effect of the present invention is as follows:
(1) overlap petal selfing insulation rubber softgel shell used and can entrust the manufacturer production of producing Capsules, cheap, and capsule shells is mainly gelatin, can be degradable at occurring in nature, and more friendly to environment;
(2) because material is light and handy, do not injure bud and bennet during use, easy to operation, operating efficiency is high;
(3) capsule shells has certain elasticity and gas permeability, and do not affect cracking and the pollination of flower pesticide, self-fruitful rate is high.
Embodiment
Below in conjunction with embodiment, the invention will be further described.
Embodiment
1) select fine individual plant: in flowering stage, select fine individual plant according to breeding objective, and make marks by pink plastic ties;
2) capsule shells prepares: the shell cap of the empty hard softgel shell of the bright yellow of 0#, 1# and 2# bought in advance and body are separated, and separated according to size, to use according to petal size fast fetching when operating;
3) petal is selected: select the large petal estimating to open in next day;
4) petal process: pending petal is taken in a have gentle hands, and another hand-held capsule shells matched with petal size, entangles petal lightly, makes capsule shells end opening cross petal the widest part, and carries out mark with marker pen at anthocaulus place;
5) management after process: to produce conventional field management method identical with capsicum.
6) treatment effect: isolate 6 flowers with cover capsule shell process average inbreeding per minute, ripening rate is 66.7%, and isolate 3,2,4 with touching colored method, sticking cloth method, solution film formation average inbreeding respectively per minute, ripening rate is respectively 63%, 53.3%, 57.7%.Visible, the work efficiency of cover capsule shell process is apparently higher than other several method.
All documents that the present invention mentions are quoted as a reference all in this application, are just quoted separately as a reference as each section of document.
Should be appreciated that and above describe for the present invention to be as set forth in the claims only example and illustrative, not to be limited it.Content of the present invention disclosed by this paper, other embodiment of the present invention is apparent for a person skilled in the art.It should be noted that this specification and embodiment only should be seen as example, actual range of the present invention is determined by claim.
Claims (4)
1. a list flower inbred isolation method for capsicum, comprises the following steps:
1) select fine individual plant: in flowering stage, select fine individual plant according to breeding objective, and mark;
2) capsule shells prepares: the shell cap of empty hard softgel shell and body are separated, described empty hard softgel shell is enteric solubility empty hard softgel shell;
3) petal is selected: select the large petal estimating to open in next day;
4) petal process: pending petal is taken in a have gentle hands, and another hand-held capsule shells matched with petal size, entangles petal lightly, makes capsule shells end opening cross petal the widest part, and carries out mark at anthocaulus place;
5) management after process: to produce conventional field management method identical with capsicum.
2. the list flower inbred isolation method of capsicum as claimed in claim 1, it is characterized in that the body oral area external diameter of described empty hard softgel shell between 6.0-7.4mm, body length is between 15.0-18.5mm.
3. the list flower inbred isolation method of capsicum as claimed in claim 1, is characterized in that described empty hard softgel shell is 0#, 1# and 2# empty hard softgel shell.
4. the list flower inbred isolation method of the capsicum as described in any one of claim 1-3, is characterized in that described empty hard softgel shell is bright yellow.
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| CN201310298007.1A CN103444509B (en) | 2013-07-16 | 2013-07-16 | A kind of list flower inbred isolation method of capsicum |
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| CN201310298007.1A CN103444509B (en) | 2013-07-16 | 2013-07-16 | A kind of list flower inbred isolation method of capsicum |
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| CN103444509A CN103444509A (en) | 2013-12-18 |
| CN103444509B true CN103444509B (en) | 2016-04-13 |
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| CN105613273A (en) * | 2016-02-01 | 2016-06-01 | 何其家 | Rapid selfing isolation method for purple sweet peppers in greenhouses |
| CN105638439A (en) * | 2016-02-01 | 2016-06-08 | 李吉菊 | Purple Hangzhou pepper quick selfing isolation method used in greenhouse |
| CN105613268A (en) * | 2016-02-01 | 2016-06-01 | 钱夕华 | Thread loop-knot type cream yellow sheep-horn pepper selfing technology |
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Effective date of registration: 20160614 Address after: 226007 Jiangsu Province, Nantong City Chongchuan District Youth Road No. 148 Patentee after: Nantong science and technology Professional School Address before: Jiangsu province Nantong City Chongchuan District 226007 building 94 Room 202, Tian Yuan Patentee before: Shen Suxiang |
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