CN106305409A - Daylily pollination method - Google Patents
Daylily pollination method Download PDFInfo
- Publication number
- CN106305409A CN106305409A CN201610704013.6A CN201610704013A CN106305409A CN 106305409 A CN106305409 A CN 106305409A CN 201610704013 A CN201610704013 A CN 201610704013A CN 106305409 A CN106305409 A CN 106305409A
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- Prior art keywords
- stigma
- pollination
- tube
- flower
- hemerocallis fulva
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Botany (AREA)
- Developmental Biology & Embryology (AREA)
- Environmental Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention relates to a daylily pollination method. The method comprises the step of sleeving a hollow light tube on a daylily stigma to isolate the stigma, wherein one end of the tube is opened, the other end is closed, and the inner diameter thereof is slightly greater than the outer diameter of the daylily stigma. The pollination result cannot be influenced whether by wind or rain, the method is extremely suitable for the hybrid pollination of the daylily, the maturing rate of the pollination of the daylily under bad weather condition is greatly improved, and the pollination time is increased. Furthermore, the method is free from emasculation, and the breeding workload is lightened.
Description
Technical field
The invention belongs to field of plant breeding, the method being specifically related to the pollination of a kind of day lily.
Background technology
The flower of hemerocallis (Hemerocallis L.) plant is three number flowers, and every flower can only be opened one day, and every flower has female
Stamen 1 piece, 6 pieces of stamen.Gynoecium is longer than stamen.As shown in Figure 2.The flower of Hemerocallis fulva L. is big, and gynoecium is elongated, if with traditional side
Method emasculation bagging hybridization, the pistil stigma after pollination can cause setting percentage to be substantially reduced with bagging friction.
Because Hemerocallis fulva L. stigma is soft, wrapping up stigma according to existing method, the covering of parcel stigma is easily blown away.Separately
During outer wrapping Hemerocallis fulva L. stigma, also it is very easy to break the Hemerocallis fulva L. stigma of jewelry, expensive clothing and other valuables, so the also uncomfortable method wrapped up.
Summary of the invention
For above-mentioned situation, after repeatedly groping in the practice of Hemerocallis fulva L. breeding, the present invention provides a kind of day lily
Hybridization pollination method.
Technical solution of the present invention is as follows:
The method of a kind of day lily pollination, is carried out stigma including being enclosed within Hemerocallis fulva L. stigma by hollow light weight tube
Isolation;Described tube one end open, the other end are closed and internal diameter is slightly larger than Hemerocallis fulva L. stigma external diameter.
Further, described tube cross section can be circular, oval, square or other polygons.
Further, described tube is made by being prone to moulding material, and further, described tube is by masking foil
Make (tinfoil set).Selecting the advantage being prone to moulding material especially masking foil is that its quality is light, it is easy to moulding and be prone to
Stigma, style are fitted, and should not be wiped off by wind.When selecting the material such as masking foil being prone to moulding, suitable chi can be cut into
Then very little size wraps up the rectangle metal object (the most blunt nosed tweezers) being slightly coarser than Hemerocallis fulva L. stigma, molding.
Further, described tube inner wall smooth.
Further, described tube is long cylindricality.
Further, described tube internal diameter is 1-3mm.
Further, a length of 6-10mm of described tube.
Further, select the flower that the same day is open during pollination, collect its pollen;The described pollen collecting time is 6:00-
10:00A.M. and 16:00-18:00P.M..
Further, described pollination object is the flower opened the same day, and pollination time is 6:00-11:30A.M..
Hemerocallis (Hemerocallis L.) plant is all suitable for by the inventive method, be particularly suited for Hemerocallis fulva L. (formal name used at school:
Hemerocallis fulva)。
Specifically, the method for above-mentioned day lily pollination, comprise the following steps:
1) open alabastrum the previous day at Hemerocallis fulva L. flowering, above-mentioned hollow light weight tube is enclosed within stigma;
2) the flowering same day, take off described hollow light weight tube, pollen is spread upon on stigma, then by light for this hollow
Matter tube (the most same hollow light weight tube or another new hollow light weight tube) is recovered on stigma;Until flower
Wilting, stigma loses pollination ability.
The present invention uses the method for sleeve pipe on stigma (or tinfoil set), no matter blows or rains, all without the knot to pollination
Fruit has any impact, is especially suitable for the hybridization pollination of hemerocallis.Day lily blooms in full summer, and florescence is frequently encountered by wind and rain
My god, the method for sleeve pipe substantially increases the setting percentage of Hemerocallis fulva L. pollination under poor weather, only opens one day for every flower
Hemerocallis fulva L. adds pollination time.Additionally, the inventive method need not emasculation, in the previous day bloomed, open alabastrum, entangle stigma i.e.
Can, alleviate breeding work amount.The transmission from one meridian to another extensive Hemerocallis fulva L. different cultivars more than 4 years and Different Ploidy hybridization practice, it was demonstrated that sleeve pipe
Method be a kind of extraordinary pollinating method to the artificial hybridization of Hemerocallis fulva L..
Accompanying drawing explanation
Fig. 1 is the single miniature tinfoil set of embodiment 1.
Fig. 2 is the Hemerocallis fulva L. flower that on embodiment 1 stigma, (at arrow) overlaps miniature tinfoil set.
Detailed description of the invention
Following example are used for illustrating the present invention, but are not limited to the scope of the present invention.
Embodiment 1
The method of day lily pollination, comprises the following steps:
1) masking foil is cut: 1.8cm × 1.8cm;
2) with the rectangle blunt nosed tweezers being slightly coarser than Hemerocallis fulva L. stigma, masking foil is made long cylindricality as shown in Figure 1, square column
Shape one section turnover is also clutched, and makes miniature tinfoil set;
3) in the previous day of Hemerocallis fulva L. flowering, open alabastrum, and miniature tin paper bag is enclosed within stigma;
4) after flowering, take off miniature tinfoil set, pollen is spread upon on stigma, more same tin paper bag is overlapped prop drawing
On head, as shown in Figure 2.
5) no longer taking off the tinfoil set on stigma, along with the wilting of flower, stigma loses pollination ability, gradually withers
Listless.
Experimental example
Be respectively adopted embodiment 1 method and traditional emasculation bagging method (stigma not sleeve pipe) to Hemerocallis fulva L. (formal name used at school:
Hemerocallis fulva) carry out contrast test of pollinating.
Test site: little Tang mountain, Changping County, Beijing district Experimental Base
Test period: 2013,2014,2015,2016.
Experimental group: the method for day lily pollination comprises the following steps:
1) open alabastrum the previous day at Hemerocallis fulva L. flowering, miniature tinfoil set same as in Example 1 is enclosed within stigma;
2) the flowering same day, take off described miniature tinfoil set, pollen is spread upon on stigma, then by same tinfoil set
Recover on stigma;Complete pollination;Until flower is wilted, stigma loses pollination ability.Wherein pollinating process includes: select the same day
Open flower, collects its pollen;The described pollen collecting time is 6:00-10:00A.M. and 16:00-18:00P.M..Described
Pollination object is the flower opened the same day, and pollination time is 6:00-11:30A.M..
3) list: label on the top set of pollination plant, label is write exactly male parent and female parent, pollination time, pollination flower
Piece number.
4) tinfoil set is removed, sowing:
Add up successfully pollination flower number, hybrid seed is taken, add up number seeds, the results are shown in Table 1.
Matched group: (emasculation bagging, sack can rub the pollen on stigma to use traditional emasculation bagging method.Because
Stigma is longer than flower), remaining step is identical with experimental group.The results are shown in Table 1.
The pollination of table 1 flower and solid situation
Although, the present invention is described in detail the most with a general description of the specific embodiments, but
On the basis of the present invention, can make some modifications or improvements it, this will be apparent to those skilled in the art.Cause
This, these modifications or improvements without departing from theon the basis of the spirit of the present invention, belong to the scope of protection of present invention.
Claims (10)
1. the method for a day lily pollination, it is characterised in that include being enclosed within Hemerocallis fulva L. stigma hollow light weight tube
Stigma is isolated;Described tube one end open, the other end are closed and internal diameter is slightly larger than Hemerocallis fulva L. stigma external diameter.
Method the most according to claim 1, it is characterised in that a length of 6-10mm of described tube, internal diameter is 1-3mm.
Method the most according to claim 1 and 2, it is characterised in that described tube cross section is circular, oval, side
Shape or other polygons.
Method the most according to claim 1 and 2, it is characterised in that described tube is made by being prone to moulding material, excellent
Elect masking foil as.
Method the most according to claim 1 and 2, it is characterised in that described tube inner wall smooth.
Method the most according to claim 1 and 2, it is characterised in that described tube is long cylindricality.
Method the most according to claim 1 and 2, it is characterised in that select the flower that the same day is open during pollination, collect its flower
Powder;The described pollen collecting time is 6:00-10:00A.M. and 16:00-18:00P.M..
Method the most according to claim 1 and 2, it is characterised in that described pollination object is the flower opened the same day, pollination
Time is 6:00-11:30A.M..
9. according to the method described in any one of claim 1-8, it is characterised in that comprise the following steps:
1) open alabastrum the previous day at Hemerocallis fulva L. flowering, described hollow light weight tube is enclosed within stigma;
2) the flowering same day, take off described hollow light weight tube, pollen is spread upon on stigma, then by this hollow light weight pipe
Shape thing is recovered on stigma;Until flower is wilted, stigma loses pollination ability.
Method the most according to claim 9, it is characterised in that described day lily is Hemerocallis fulva L..
Priority Applications (1)
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CN201610704013.6A CN106305409A (en) | 2016-08-22 | 2016-08-22 | Daylily pollination method |
Applications Claiming Priority (1)
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CN201610704013.6A CN106305409A (en) | 2016-08-22 | 2016-08-22 | Daylily pollination method |
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CN106305409A true CN106305409A (en) | 2017-01-11 |
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CN201610704013.6A Pending CN106305409A (en) | 2016-08-22 | 2016-08-22 | Daylily pollination method |
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN112136682A (en) * | 2020-09-17 | 2020-12-29 | 云南农业大学 | Method for creating new germplasm by wild distant hybridization of rhododendron |
CN112806256A (en) * | 2021-02-19 | 2021-05-18 | 杭州市农业科学研究院 | Simple method for selfing and pollinating eggplants |
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CN101107917A (en) * | 2007-08-20 | 2008-01-23 | 成都市第一农业科学研究所 | Cowpea isolation hybridization pollination method |
CN203435499U (en) * | 2013-07-31 | 2014-02-19 | 江苏沿海地区农业科学研究所 | Cotton cross breeding stigma casing |
CN104823831A (en) * | 2015-04-13 | 2015-08-12 | 新疆农垦科学院 | Sunflower inbred line single flower isolation breeding method |
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2016
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Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
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CN101107917A (en) * | 2007-08-20 | 2008-01-23 | 成都市第一农业科学研究所 | Cowpea isolation hybridization pollination method |
CN203435499U (en) * | 2013-07-31 | 2014-02-19 | 江苏沿海地区农业科学研究所 | Cotton cross breeding stigma casing |
CN104823831A (en) * | 2015-04-13 | 2015-08-12 | 新疆农垦科学院 | Sunflower inbred line single flower isolation breeding method |
Non-Patent Citations (4)
Title |
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丁寿康: "杂交育种(续)", 《农业科技通讯》 * |
危家文等: "杂交棉不去雄人工制种技术研究", 《湖南文理学院学报(自然科学版)》 * |
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Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN112136682A (en) * | 2020-09-17 | 2020-12-29 | 云南农业大学 | Method for creating new germplasm by wild distant hybridization of rhododendron |
CN112136682B (en) * | 2020-09-17 | 2021-05-04 | 云南农业大学 | Method for creating new germplasm by wild distant hybridization of rhododendron |
CN112806256A (en) * | 2021-02-19 | 2021-05-18 | 杭州市农业科学研究院 | Simple method for selfing and pollinating eggplants |
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Application publication date: 20170111 |