CN103828711B - Capsicum hybrid seeding labeling method - Google Patents
Capsicum hybrid seeding labeling method Download PDFInfo
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- CN103828711B CN103828711B CN201410101133.8A CN201410101133A CN103828711B CN 103828711 B CN103828711 B CN 103828711B CN 201410101133 A CN201410101133 A CN 201410101133A CN 103828711 B CN103828711 B CN 103828711B
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Abstract
The present invention relates to plant hybridization seeding technique field, specifically a kind of capsicum hybrid seeding labeling method; Comprise the steps: that (1) determines capsicum hybridization pollination period; (2) the maternal plant mark that hybrid seeding is selected; (3) parent sire of hybrid pigs pollen collection; (4) emasculation hybridization is isolated with cover flower; (5) hybridization flower position mark; (6) fruit mark is planted; (7) fruit picking and process; The capsicum hybrid seed of production of hybrid seeds labeling method gained of the present invention, can stop kind fruit picking and mix, and ensures the purity of seed, can guarantee the uniformity that the correctness of later stage experimental study is planted with production.
Description
Technical field
The present invention relates to plant hybridization seeding technique field, specifically a kind of capsicum hybrid seeding labeling method.
Background technology
Capsicum, be a kind of Chang Yihua crop, androgyny, mostly generally is selfing, and simultaneously because of anemophily and the impact of entomophila factor, different colored hybrid rate is also higher, can reach 15 ~ 20% and more than.On capsicum production is planted, present stage transforms except green pepper fruit has individual applications to adopt routine to plant the use of outer polycross kind.Produce in capsicum, test and carry out in hybrid seeding in order to multiple merit gene transformation is polymerized, also because now often gathering when to gather kind other allocarpy that green pepper fruit is mixed into selfed seed fruit and produces because of anemophily or entomophila factor by labeling method defect, and cannot check when seed collecting species test, bring production to plant and test the variet complexity of the production of hybrid seeds, impact production and experimental study, particularly carry out hybrid seeding in the capsicum test production of hybrid seeds and excellent genes transformation, to bring to experimental study because of really error of gathering sometimes and have a strong impact on.The labeling method of capsicum hybrid seeding is summed up in research, stops the variet complexity of hybrid seeding, ensures the purity of seed, can guarantee the correctness of the uniformity that later stage production is planted and experimental study, can accelerate the sustainable development advancing capsicum industry.
Summary of the invention
The object of this invention is to provide a kind of capsicum hybrid seeding labeling method, it finally can be stopped variet complexity when capsicum hybrid seeding is gathered kind, ensure the purity of seed, guarantees the uniformity of later stage productions kind and the correctness of experimental study.
For achieving the above object, the technical solution used in the present invention is: a kind of capsicum hybrid seeding labeling method, comprises the steps:
(1) capsicum hybridization pollination period is determined;
(2) the maternal plant mark that hybrid seeding is selected;
(3) parent sire of hybrid pigs pollen collection;
(4) emasculation hybridization is isolated with cover flower;
(5) hybridization flower position mark;
(6) fruit mark is planted;
(7) fruit picking and process;
Described step (1) determines that capsicum hybridization pollination be period is hybridization pollination period in flowering stage of capsicum maternal plant 3rd ~ 6 flowers;
The maternal plant mark that described step (2) hybrid seeding is selected, comprises the steps: in the pollinated stage of step (1), performs mark with colored drop hanging on the maternal plant branch of the production of hybrid seeds of selecting and remain;
Described step (3) parent sire of hybrid pigs pollen collection, comprise the steps: the planned production of hybrid seeds be combined in pollination before 1d to pluck maturation but the male parent flower pesticide of also not blooming afternoon, template/the bag of male parent title is had to wrap with mark, be placed in dry place, indoor calm cool place to spread out, allow after flower pesticide nature loose powder and gather pollen (also can picking flowers medicine by that morning of pollination in afternoon, noon gathers pollen after loose powder);
Described step (4) emasculation hybridization is isolated with cover flower, comprise the steps: that ripe on the plant that step (2) is selected but the petal of not blooming and androecium remove, the paternal pollen banister bruss that step (3) gathers to be coated on the chapiter after emasculation (female parent be male sterile there is not emasculation directly pollinate) by production of hybrid seeds plan, bennet place paper using adhesive plaster after pollination is that trumpet type clings, pollination flower is wrapped in loudspeaker, prevent because of entomophila or anemophily a string of flowers pollination (female parent is male sterile, can isolating without adhesive plaster of other capsicum material is not planted) with field, and record pollination situation,
Described step (5) hybridization flower position mark, comprises the steps:
What 1. maternal individual plant only hybridized 1 male parent pinches half by the leaf hand of the pollination of step (4) flower leaf position, place, stays half leaf on plant, and indicate hybrid combination title with marking pen on pollination plant drop;
What 2. maternal individual plant hybridized multiple male parent pinches half by the leaf hand of the pollination of step (4) flower leaf position, place, stay the half blade face on plant is numbered by parent with gel ink pen mark after the hybrid combination code name that forms of double figures;
Described step (6) plants fruit mark, comprises the steps:
1. maternal individual plant only hybridizes 1 male parent, and after pollination, the young fruit of step (5) half leaf mark position is smeared mark with marking pen by 8 ~ 10d on carpopodium;
2. maternal individual plant hybridizes multiple male parent, and the shaping fruit contrast that step (5) mark position is entered green mature by 15 ~ 20d after pollination stays half leaf marks plant hybrid combination code name marking pen at its fruit face mark hybrid combination code name;
(7) fruit picking of described step and process, after comprising the steps: that maturation of bearing fruit is spent in pollination after step (4) emasculation hybridization, according to the production of hybrid seeds situation of hybrid combination, preparation is gathered bag, indicates the number-mark that the production of hybrid seeds is combined, and checks the number and plucks hybrid combination green pepper fruit, green pepper fruit is transported back after indoor recheck mark and collects seed, son of collecting seed eluriate impurity elimination and be placed on and directly shine aeration-drying place without strong daylight and dry in the shade, obtain hybrid seed, complete production of hybrid seeds process.
Advantageous Effects of the present invention is: the seed that the capsicum crossbreed fruit adopting labeling method of the present invention to select and remain is gathered, the variet complexity in capsicum hybrid seeding process can be stopped, ensure the purity of seed, guarantee the correctness of the uniformity that later stage production is planted and experimental study.
Embodiment
Below in conjunction with embodiment, the present invention is described further;
Embodiment 1
Capsicum hybrid seeding labeling method, comprises the steps:
1) capsicum hybridization pollination best period, is determined: spend flower in full bloom to begin as the best initial time of hybridization pollination for the 3rd the male sterile female parent material plant of capsicum " 9537 ".
2) the maternal plant mark that, hybrid seeding is selected: perform mark with colored drop hanging on the branch at the obvious place of " 9537 " maternal plant of the production of hybrid seeds of selecting and remain, drop indicates maternal numbering " 9537 " with marking pen.
3), parent sire of hybrid pigs pollen collection: before the planned production of hybrid seeds is combined in pollination, 1d to pluck maturation but the flower pesticide of the male parent " 4536 " of also not blooming afternoon, wrap with the template (bag) of mark " 4536 " title, be placed in dry place, indoor calm cool place to spread out, allow flower pesticide nature loose powder after gather pollen.
4), pollination hybridization is isolated with cover flower: be coated in by " 4536 " the paternal pollen banister bruss gathered on the chapiter of selected " 9537 " maternal plant mature flower, bennet place paper using adhesive plaster after pollination is that trumpet type clings, pollination flower is wrapped in loudspeaker, prevents because of entomophila or the pollination of anemophily a string of flowers; Can isolating without bagging of other capsicum material beyond male parent is implanted with without planting with field.Minute book records pollination situation.
5), hybridization flower position mark: the leaf hand of pollination flower leaf position, place is pinched half, stays half leaf on plant, and indicate hybrid combination title " 9537 × 4536 " with marking pen on pollination plant drop.
6) fruit mark, is planted: the young fruit of half leaf mark position is smeared mark with marking pen by 8d on carpopodium after pollination.
7), fruit picking and process: after hybridization pollination flower bears fruit maturation, prepare fruit picking bag, indicate production of hybrid seeds combination title " 9537 × 4536 " mark, check the number and pluck markd green pepper fruit on carpopodium, gather after rechecking mark after green pepper fruit transports indoor back and collect seed, institute's sub-elutriation impurity elimination of collecting seed is placed on and directly shines aeration-drying place without strong daylight and dry in the shade.Obtain the seed of hybrid combination " 9537 × 4536 ".
Embodiment 2
Capsicum hybrid seeding labeling method, comprises the steps:
1) capsicum hybridization pollination best period, is determined: spend flower in full bloom to begin as the hybridization pollination best start phase capsicum " 4631 " maternal plant the 4th.
2) the maternal plant mark that, hybrid seeding is selected: perform mark with colored drop hanging on the branch at the obvious place of " 4631 " maternal plant of the production of hybrid seeds of selecting and remain, drop indicates maternal numbering " 4631 " with marking pen.
3), parent sire of hybrid pigs pollen collection: before the planned production of hybrid seeds is combined in pollination, 1d to pluck maturation but the flower pesticide of the male parent " 5326 " of also not blooming afternoon, the template of " 5326 " male parent title (bag) is had to wrap with mark, be placed in dry place, indoor calm cool place to spread out, allow flower pesticide nature loose powder after gather pollen.
4), emasculation hybridization isolate with cover flower: by maturation on selected " 4631 " maternal plant but the petal of not blooming and androecium remove, " 5326 " the paternal pollen banister bruss gathered is coated on the chapiter after emasculation, then the bennet place paper using adhesive plaster after pollination is that trumpet type clings, pollination flower is wrapped in loudspeaker, prevents because of entomophila or the pollination of anemophily a string of flowers.Minute book records pollination situation.
5), maternal individual plant only hybridizes the hybridization flower position mark of 1 male parent: the leaf hand of pollination flower leaf position, place is pinched half, stays half leaf on plant, and indicate hybrid combination title " 4631 × 5326 " with marking pen on pollination plant drop.
6) fruit mark, is planted: the young fruit of half leaf mark position is smeared mark with marking pen by 10d on carpopodium after pollination.
7), fruit picking and process: after hybridization pollination flower bears fruit maturation, preparation is gathered bag, indicate production of hybrid seeds combination title " 4631 × 5326 " mark, check the number and pluck markd green pepper fruit on carpopodium, gather after rechecking mark after green pepper fruit transports indoor back and collect seed, institute's sub-elutriation impurity elimination of collecting seed is placed on and directly shines aeration-drying place without strong daylight and dry in the shade.Obtain the seed of hybrid combination " 4631 × 5326 ".
Embodiment 3
Capsicum hybrid seeding labeling method, comprises the steps:
1) capsicum hybridization pollination best period, is determined: spending flower in full bloom to begin capsicum " 4637 " maternal plant the 5th is hybridization pollination best start period.
2) the maternal plant mark that, hybrid seeding is selected: perform mark with colored drop hanging on the branch at the obvious place of " 4637 " maternal plant of the production of hybrid seeds of selecting and remain, drop indicates maternal numbering " 4637 " with marking pen.
3), parent sire of hybrid pigs pollen collection: before the planned production of hybrid seeds is combined in pollination, 1d to pluck maturation but the flower pesticide of the male parent " 4307 " of also not blooming afternoon, the template of " 4307 " male parent title (bag) is had to wrap with mark, be placed in dry place, indoor calm cool place to spread out, allow flower pesticide nature loose powder after gather pollen.
4), emasculation hybridization isolate with cover flower: by maturation on selected " 4637 " maternal plant but the petal of not blooming and androecium remove, " 4307 " the paternal pollen banister bruss gathered is coated on the chapiter after emasculation, then the bennet place paper using adhesive plaster after pollination is that trumpet type clings, pollination flower is wrapped in loudspeaker, prevents because of entomophila or the pollination of anemophily a string of flowers.Minute book records pollination situation.
5), maternal individual plant hybridizes the hybridization flower position mark of multiple male parent: the leaf hand of pollination flower leaf position, place is pinched half, stay half leaf on plant, and stay on the half blade face on plant with gel ink pen indicate by hybrid strain number after double figures form hybrid combination numbering " 3707 ".
6), fruit mark is planted: the shaping fruit contrast that half leaf mark position is entered green mature by 15d after pollination stays the hybrid combination code name that half blade face marks plant, with marking pen its fruit face mark hybrid combination code name " 3707 ".
7), fruit picking and process: after hybridization pollination flower bears fruit maturation, preparation is gathered bag, indicate production of hybrid seeds combination code name " assorted 3707 " mark, check the number and pluck markd green pepper fruit on fruit face, gather after rechecking mark after green pepper fruit transports indoor back and collect seed, institute's sub-elutriation impurity elimination of collecting seed is placed on and directly shines aeration-drying place without strong daylight and dry in the shade.Obtain the seed of hybrid combination " assorted 3707 ".
Embodiment 4
Capsicum hybrid seeding labeling method, comprises the steps:
1) capsicum hybridization pollination best period, is determined: spending flower in full bloom to begin capsicum " 4637 " maternal plant the 6th is hybridization pollination best start period.
2) the maternal plant mark that, hybrid seeding is selected: perform mark with colored drop hanging on the branch at the obvious place of " 4637 " maternal plant of the production of hybrid seeds of selecting and remain, drop indicates maternal numbering " 4637 " with marking pen.
3), parent sire of hybrid pigs pollen collection: before the planned production of hybrid seeds is combined in pollination, 1d to pluck maturation but the flower pesticide of the male parent " 5412 " of also not blooming afternoon, the template of " 5412 " male parent title (bag) is had to wrap with mark, be placed in dry place, indoor calm cool place to spread out, allow flower pesticide nature loose powder after gather pollen.
4), emasculation hybridization isolate with cover flower: by maturation on selected " 4637 " maternal plant but the petal of not blooming and androecium remove, " 5412 " the paternal pollen banister bruss gathered is coated on the chapiter after emasculation, then the bennet place paper using adhesive plaster after pollination is that trumpet type clings, pollination flower is wrapped in loudspeaker, prevents because of entomophila or the pollination of anemophily a string of flowers.Minute book records pollination situation.
5), maternal individual plant hybridizes the hybridization flower position mark of multiple male parent: the leaf hand of pollination flower leaf position, place is pinched half, stay half leaf on plant, and stay on the half blade face on plant with gel ink pen indicate by hybrid strain number after double figures form hybrid combination numbering " 3712 ".
6), fruit mark is planted: the shaping fruit contrast that half leaf mark position is entered green mature by 20d after pollination stays the hybrid combination code name that half blade face marks plant, with marking pen its fruit face mark hybrid combination code name " 3712 ".
7), fruit picking and process: after hybridization pollination flower bears fruit maturation, preparation is gathered bag, indicate production of hybrid seeds combination code name " assorted 3712 " mark, check the number and pluck markd green pepper fruit on fruit face, gather after rechecking mark after green pepper fruit transports indoor back and collect seed, institute's sub-elutriation impurity elimination of collecting seed is placed on and directly shines aeration-drying place without strong daylight and dry in the shade.Obtain the seed of hybrid combination " assorted 3712 ".
The foregoing is only preferred embodiment of the present invention, not in order to limit the present invention, within the spirit and principles in the present invention all, any amendment done, equivalent replacement, improvement etc., all should be included within protection scope of the present invention.
Claims (1)
1. capsicum hybrid seeding labeling method, is characterized in that, comprises the steps:
(1) capsicum hybridization pollination period is determined;
(2) the maternal plant mark that hybrid seeding is selected;
(3) parent sire of hybrid pigs pollen collection;
(4) emasculation hybridization is isolated with cover flower;
(5) hybridization flower position mark;
(6) fruit mark is planted;
(7) fruit picking and process;
Described step (1) determines that capsicum hybridization pollination be period is hybridization pollination period in flowering stage of capsicum maternal plant 3rd ~ 6 flowers;
The maternal plant mark that described step (2) hybrid seeding is selected, comprises the steps: in the pollinated stage of step (1), performs mark with colored drop hanging on the maternal plant branch of the production of hybrid seeds of selecting and remain;
Described step (3) parent sire of hybrid pigs pollen collection, comprise the steps: the planned production of hybrid seeds be combined in pollination before 1d to pluck maturation but the male parent flower pesticide of also not blooming afternoon, template/the bag of male parent title is had to wrap with mark, be placed in dry place, indoor calm cool place to spread out, allow flower pesticide nature loose powder after gather pollen;
Described step (4) emasculation hybridization is isolated with cover flower, comprise the steps: that ripe on the plant that step (2) is selected but the petal of not blooming and androecium remove, by production of hybrid seeds plan, the paternal pollen banister bruss that step (3) gathers is coated on the chapiter after emasculation, bennet place paper using adhesive plaster after pollination is that trumpet type clings, pollination flower is wrapped in loudspeaker, prevent because of entomophila or the pollination of anemophily a string of flowers, and record pollination situation;
Described step (5) hybridization flower position mark, comprises the steps:
What 1. maternal individual plant only hybridized 1 male parent pinches half by the leaf hand of the pollination of step (4) flower leaf position, place, stays half leaf on plant, and indicate hybrid combination title with marking pen on pollination plant drop;
What 2. maternal individual plant hybridized multiple male parent pinches half by the leaf hand of the pollination of step (4) flower leaf position, place, stay the half blade face on plant is numbered by parent with gel ink pen mark after the hybrid combination code name that forms of double figures;
Described step (6) plants fruit mark, comprises the steps:
1. maternal individual plant only hybridizes 1 male parent, and after pollination, the young fruit of step (5) half leaf mark position is smeared mark with marking pen by 8 ~ 10d on carpopodium;
2. maternal individual plant hybridizes multiple male parent, and the shaping fruit contrast that step (5) mark position is entered green mature by 15 ~ 20d after pollination stays half leaf marks plant hybrid combination code name marking pen at its fruit face mark hybrid combination code name;
(7) fruit picking of described step and process, after comprising the steps: that maturation of bearing fruit is spent in pollination after step (4) emasculation hybridization, according to the production of hybrid seeds situation of hybrid combination, preparation is gathered bag, indicates the number-mark that the production of hybrid seeds is combined, and checks the number and plucks hybrid combination green pepper fruit, green pepper fruit is transported back after indoor recheck mark and collects seed, son of collecting seed eluriate impurity elimination and be placed on and directly shine aeration-drying place without strong daylight and dry in the shade, obtain hybrid seed, complete production of hybrid seeds process.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1176736A (en) * | 1997-09-12 | 1998-03-25 | 开封市蔬菜科学研究所 | Method for breeding hybridized cayence pepper |
| CN1781358A (en) * | 2004-11-29 | 2006-06-07 | 中国农业大学 | Method for producing hot pepper hybrid seed and appraising purity |
| CN101011022A (en) * | 2007-01-30 | 2007-08-08 | 广东省农业科学院蔬菜研究所 | Seed selection method of capsicum variety |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100504172B1 (en) * | 2003-07-01 | 2005-07-28 | 재단법인서울대학교산학협력재단 | Method for breeding a hybrid pepper using interspecific hybridization |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1176736A (en) * | 1997-09-12 | 1998-03-25 | 开封市蔬菜科学研究所 | Method for breeding hybridized cayence pepper |
| CN1781358A (en) * | 2004-11-29 | 2006-06-07 | 中国农业大学 | Method for producing hot pepper hybrid seed and appraising purity |
| CN101011022A (en) * | 2007-01-30 | 2007-08-08 | 广东省农业科学院蔬菜研究所 | Seed selection method of capsicum variety |
Non-Patent Citations (2)
| Title |
|---|
| 改良早丰辣椒杂交制种技术研究;吕中华等;《西南农业学报》;19941231;第7卷(第1期);第110-114页 * |
| 辣椒03-10-6组合杂交制种技术规程;余康宁等;《农技服务》;20111231;第28卷(第10期);第1403-1404页,尤其是第5节 * |
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Inventor after: Yang Hong Inventor after: Jiang Hong Inventor after: Lai Wei Inventor after: Liu Chongzheng Inventor after: Su Dan Inventor after: Mou Yumei Inventor before: Yang Hong Inventor before: Jiang Hong |
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