CN104082130B - A kind of method of quick location Fructus Capsici selfing fruit - Google Patents
A kind of method of quick location Fructus Capsici selfing fruit Download PDFInfo
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- CN104082130B CN104082130B CN201410368568.9A CN201410368568A CN104082130B CN 104082130 B CN104082130 B CN 104082130B CN 201410368568 A CN201410368568 A CN 201410368568A CN 104082130 B CN104082130 B CN 104082130B
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- Prior art keywords
- fine rule
- holder
- flower
- fruit
- labelling
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- 235000013399 edible fruits Nutrition 0.000 title claims abstract description 27
- 238000000034 method Methods 0.000 title claims abstract description 13
- 230000010152 pollination Effects 0.000 claims abstract description 18
- 238000002372 labelling Methods 0.000 claims abstract description 14
- 241000196324 Embryophyta Species 0.000 abstract description 11
- 238000009395 breeding Methods 0.000 abstract description 2
- 230000001488 breeding effect Effects 0.000 abstract description 2
- 238000005516 engineering process Methods 0.000 abstract description 2
- 238000000465 moulding Methods 0.000 description 3
- 238000003976 plant breeding Methods 0.000 description 2
- 235000002566 Capsicum Nutrition 0.000 description 1
- 235000002568 Capsicum frutescens Nutrition 0.000 description 1
- 239000006002 Pepper Substances 0.000 description 1
- 235000016761 Piper aduncum Nutrition 0.000 description 1
- 235000017804 Piper guineense Nutrition 0.000 description 1
- 244000203593 Piper nigrum Species 0.000 description 1
- 235000008184 Piper nigrum Nutrition 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
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- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The method that the invention discloses a kind of quick location Fructus Capsici selfing fruit, belongs to field of plant variety breeding technology. First selected selfing individual plant also carries out listing mark; Secondly open flower it is about in selected labeled individual plant in 12h; The pin hole front end of certain specification 0.2-0.4mm sewing-needle is cut off, forms dished structure, other end needle point is cut off simultaneously; Being inserted in by fine rule in dished hole, fine rule penetrates the holder position being about to open flower in 12h together with dished structure; Cut the fine rule of long 2-3cm in holder as the labelling of pollination self flower; Manually the flower after labelling is pollinated; The fruit of fine rule labelling is had in physiological maturity results holder.
Description
Technical field
A kind of method of quick location Fructus Capsici selfing fruit, belong to field of plant breeding, more specifically one is incorporated into menu strain, selected flower, makes lead-in wire utensil, lead-in wire utensil traction fine rule stays holder, artificial autocopulation's pollination, results physiological maturity fruit through holder, fine rule, reaches quickly to position the purpose of selfing fruit.
Background technology
Fructus Capsici belongs to Constantly allogamous plant, and its purification typically requires artificial autocopulation's pollination. Before artificial autocopulation's pollination, it is necessary to the flower being about to pollination is carried out labelling, frequently with the method listed, it is about to be inserted in holder with the little fine rule moulding board. The method shortcoming is in that moulding board has constant weight, and holder of tying tight on the one hand causes the obstacle of holder transmission nutrient substance, affects the normal growth of fruit; On the other hand, when ambient wind is bigger, holder under wind-force effect, can be shut down, cause pollination failures by little board of moulding, it is impossible to obtains Fructus Capsici selfing fruit; The method positioning selfing flower additionally, list is also required to more artificial and lists the time, reduces pollination efficiency.
Summary of the invention
The method of a kind of quick location Fructus Capsici selfing fruit has many superioritys, and one is artificial and consuming time few; Two is with low cost; Three are easy to distinguish the pepper fruit of selfing and non-selfing.
The present invention is achieved in that
1. the method for a quick location Fructus Capsici selfing fruit, belong to field of plant breeding, more specifically one is incorporated into menu strain, selected flower, makes lead-in wire utensil, lead-in wire utensil traction fine rule stays holder, artificial autocopulation's pollination, results physiological maturity fruit through holder, fine rule, reaches quickly to position the purpose of selfing fruit..
2. for achieving the above object, the method for the present invention mainly includes following seven steps:
1. selected individual plant: be selected in and need the individual plant of selfing and carry out listing mark;
2. selected flower: be about to open flower in selected labeled individual plant in 12h;
3. make lead-in wire utensil: cut off the pin hole front end of certain specification 0.2-0.4mm sewing-needle, form dished structure, other end needle point is cut off simultaneously;
4. fine rule traverse holder: fine rule is inserted in dished hole, fine rule penetrates, together with dished lead-in wire utensil, the holder position being about to open flower in 12h;
5. fine rule stays holder: cut the fine rule of long 2-3cm in holder as the labelling of pollination self flower;
6. artificial autocopulation's pollination: manually the flower after labelling is carried out pollination self;
7. results physiological maturity fruit: have the fruit of fine rule labelling in physiological maturity results holder.
Beneficial effects of the present invention: one is with low cost. After Herba Damnacanthi pin hole is cut off, needle point cuts, it is possible to recycling, and only expending the fine rule of 2-3cm length, price is low every time; Two is artificial and consuming time few. Have only to be inserted in fine rule in the utensil of dished structure; Three is that labelling is accurate, short and sweet. Fine rule, owing to being fixed in holder, will not come off with the wind, is also easy to the fruit of fine rule and the fruit without fine rule will be had to be distinguished during results fruit.
Detailed description of the invention:
Below in conjunction with specific embodiment, the present invention is conducted further description.
Embodiment
1) select fine individual plant: in florescence, select excellent dense purple chili 7036 individual plant according to breeding objective, and hang white at place of its main split and mould board and be marked;
2) selected flower: be about to 5 open flowers in selected labeled individual plant in 12h;
3) make lead-in wire utensil: cut off the pin hole front end of 0.4mm sewing-needle, form dished structure, other end needle point is cut off simultaneously;
4) fine rule traverse holder: being inserted in by fine rule in dished hole, fine rule penetrates the holder position being about to open flower together with dished structure;
5) fine rule stays holder: the end 2cm of fine rule stays the labelling as pollination self flower in holder;
6) artificial autocopulation's pollination: manually the flower after labelling is carried out pollination self;
7) results physiological maturity fruit: having the fruit 4 of fine rule labelling in physiological maturity results holder, success rate reaches 80%;
8) checking: sow the Some seeds 40 of above-mentioned 4 fruits, checks the color of blade at cotyledon period, and 40 individual plants are all dense purple, it was shown that said method succeeds.
Claims (1)
1. the method for a quick location Fructus Capsici selfing fruit, it is characterized in that, include selected individual plant, selected flower in described method, make lead-in wire utensil, lead-in wire utensil traction fine rule stays holder, artificial autocopulation's pollination, results seven steps of physiological maturity fruit through holder, fine rule, reaching quickly to position the purpose of selfing fruit, seven described step concrete operations are as follows:
1. selected individual plant: be selected in and need the individual plant of selfing and carry out listing mark;
2. selected flower: be about to open flower in selected labeled individual plant in 12h;
3. make lead-in wire utensil: cut off the pin hole front end of certain specification 0.2-0.4mm sewing-needle, form dished structure, other end needle point is cut off simultaneously;
4. fine rule traverse holder: fine rule is inserted in dished hole, fine rule penetrates, together with dished lead-in wire utensil, the holder position being about to open flower in 12h;
5. fine rule stays holder: cut the fine rule of long 2-3cm in holder as the labelling of pollination self flower;
6. artificial autocopulation's pollination: manually the flower after labelling is carried out pollination self;
7. results physiological maturity fruit: have the fruit of fine rule labelling in physiological maturity results holder.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410368568.9A CN104082130B (en) | 2014-07-30 | 2014-07-30 | A kind of method of quick location Fructus Capsici selfing fruit |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410368568.9A CN104082130B (en) | 2014-07-30 | 2014-07-30 | A kind of method of quick location Fructus Capsici selfing fruit |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104082130A CN104082130A (en) | 2014-10-08 |
| CN104082130B true CN104082130B (en) | 2016-06-15 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410368568.9A Expired - Fee Related CN104082130B (en) | 2014-07-30 | 2014-07-30 | A kind of method of quick location Fructus Capsici selfing fruit |
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Families Citing this family (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105613261A (en) * | 2016-02-01 | 2016-06-01 | 钱夕刚 | Thread loop-knot type deep yellow bell pepper selfing technology |
| CN105519428A (en) * | 2016-02-01 | 2016-04-27 | 李吉菊 | Thread double-knot type purple Hangzhou pepper selfing technology |
| CN105613270A (en) * | 2016-02-01 | 2016-06-01 | 钱夕华 | Rapid selfing isolation method for cream yellow cow-horn peppers in greenhouses |
| CN105638441A (en) * | 2016-02-01 | 2016-06-08 | 钱夕华 | Thin thread double-knot purple thin-peel pepper selfing technology |
| CN105454038A (en) * | 2016-02-01 | 2016-04-06 | 钱夕华 | Fine thread double-knot type dark-yellow-skin capsicum selfing technology |
| CN105638443A (en) * | 2016-02-01 | 2016-06-08 | 钱夕华 | Purple goat horn pepper quick selfing isolation method used in greenhouse |
| CN105660370A (en) * | 2016-02-01 | 2016-06-15 | 钱夕华 | Method for rapid selfing isolation of purple bull-horn pepper in greenhouse |
| CN105638440A (en) * | 2016-02-01 | 2016-06-08 | 李吉菊 | Cream yellow Hangzhou pepper quick selfing isolation method used in greenhouse |
| CN105613267A (en) * | 2016-02-01 | 2016-06-01 | 李吉菊 | Rapid selfing isolation method for red Hangzhou peppers in greenhouses |
| CN105532436A (en) * | 2016-02-01 | 2016-05-04 | 李吉菊 | Fine line loop knot type red-skin Hangzhou pepper selfing technology |
| CN105454040A (en) * | 2016-02-01 | 2016-04-06 | 钱夕华 | Rapid selfing isolation method for red-skin capsicum in greenhouse |
| CN105684886A (en) * | 2016-02-01 | 2016-06-22 | 钱夕华 | Inbreeding technique of filament double-knot type cream yellow thin skin pepper |
| CN105613262A (en) * | 2016-02-01 | 2016-06-01 | 钱夕刚 | Thread loop-knot type purple bell pepper selfing technology |
| CN105532437A (en) * | 2016-02-01 | 2016-05-04 | 李吉菊 | Fine line loop knot type purple cayenne pepper selfing technology |
| CN105532434A (en) * | 2016-02-01 | 2016-05-04 | 钱夕刚 | Fine line loop knot type red-skin pod pepper selfing technology |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102273402A (en) * | 2011-06-30 | 2011-12-14 | 杭州市农业科学研究院 | Pepper inbred isolation method |
| CN102524053A (en) * | 2012-01-16 | 2012-07-04 | 广东省农业科学院蔬菜研究所 | Seed production method for hybrid pepper variety |
| CN103444509A (en) * | 2013-07-16 | 2013-12-18 | 沈素香 | Single flower selfing isolation method for small chili |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8816170B2 (en) * | 2011-07-22 | 2014-08-26 | Enza Zaden Beheer B.V. | Pepper hybrid E 490264 |
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2014
- 2014-07-30 CN CN201410368568.9A patent/CN104082130B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102273402A (en) * | 2011-06-30 | 2011-12-14 | 杭州市农业科学研究院 | Pepper inbred isolation method |
| CN102524053A (en) * | 2012-01-16 | 2012-07-04 | 广东省农业科学院蔬菜研究所 | Seed production method for hybrid pepper variety |
| CN103444509A (en) * | 2013-07-16 | 2013-12-18 | 沈素香 | Single flower selfing isolation method for small chili |
Non-Patent Citations (2)
| Title |
|---|
| S.O. Moreira et.al.,.Phenotypic and genotypic variation among Capsicum annuum recombinant inbred lines resistant to bacterial spot.《Genetics and Molecular Research》.2013,第12卷(第2期),第1232-1242页. * |
| 隋益虎等.辣椒雄性不育系1110A的选育.《安徽科技学院学报》.2013,第27卷(第2期),第14-17页. * |
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| CN104082130A (en) | 2014-10-08 |
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Inventor after: Li Lanhua Inventor before: Hu Nengbing Inventor before: Sui Yihu Inventor before: Su Zhaolong Inventor before: Zhan Xuewen |
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