CN105660368A - Thread dual-knot type selfing technology for red sweet pepper - Google Patents
Thread dual-knot type selfing technology for red sweet pepper Download PDFInfo
- Publication number
- CN105660368A CN105660368A CN201610067609.XA CN201610067609A CN105660368A CN 105660368 A CN105660368 A CN 105660368A CN 201610067609 A CN201610067609 A CN 201610067609A CN 105660368 A CN105660368 A CN 105660368A
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- Prior art keywords
- flower
- selfing
- fructus capsici
- fine rule
- bennet
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
Abstract
The invention discloses a thread dual-knot type selfing technology for red sweet pepper and belongs to the technical field of plant breeding. Firstly, single plants of red sweet pepper for selfing are selected and tags are hung on the single plants for marking; then flowers coming into blossom are selected from marked single plants; household white threads are knotted to form loops with a dual single-knot method, loops at one end sleeve pedicels and are slightly tightened, and threads 2 cm long are reserved at the other end; the threads and the flowers are covered with adhesive stickers and upper and lower ends are pinched; fruits marked with the white loops are harvested in the physiological mature period.
Description
Technical field
The invention discloses a kind of fine rule double-junction Folium Styracis Suberifoliae Fructus Capsici selfing technology, belong to field of plant variety breeding technology. First selected Folium Styracis Suberifoliae Fructus Capsici selfing individual plant also carries out listing mark; Next selects, and labelling individual plant is interior is about to open flower; Adopting dual unijunction method by domestic white fine rule formation of knots, the coil of one end is enclosed within bennet, tightens up slightly, and the other end leaves the fine rule of 2cm length; With sticky label paper, fine rule and flower are wrapped and clutch in top and bottom; Physiological maturity results hang the fruit of white line circle labelling.
Background technology
Fructus Capsici belongs to Constantly allogamous plant, and its purification typically requires artificial pollination. There is shortcomings in said method, one is that pollination speed is slow. From rotating and push aside flower, picking pollen, bread at least need 20-30s in stigma, the operation such as list; Two is that pollination efficiency is low. Adopting in 4-5 month and manually pollinate, for Capsicum frutescens L.Var.1o m Bail, Cornu Caprae seu Ovis green pepper, line green pepper, screw green pepper, capsicum annum fasciculatum, thin skin green pepper, its success rate is up to more than 80%, once enter even 6-7 month in 5-6 month, pollination efficiency is substantially reduced; And manually Fructus Capsici is pollinated adopting in 4-5 month, its pollination rate is also only 40-50%; Three is be easily caused biology hybrid. Artificial pollination many employings dissecting needle or syringe needle, as inoculating tool, when different Fructus Capsici selfing linies are inoculated, easily remain the pollen of previous selfing line at instrument leading portion, are easily caused biology hybrid or impure for the pollination of later selfing line.
Summary of the invention
A kind of fine rule double-junction Folium Styracis Suberifoliae Fructus Capsici selfing technology has many superioritys, and one is artificial and consuming time few; Two is that cost is extremely cheap; Three are easy to distinguish selfing or non-selfing fruit. According to above-mentioned cognition and experience, we have invented the quick inbred isolation method of Folium Styracis Suberifoliae Fructus Capsici in a kind of greenhouse.
The present invention is achieved in that
A kind of fine rule double-junction Folium Styracis Suberifoliae Fructus Capsici selfing technology, it is characterized in that, described method includes selected Folium Styracis Suberifoliae Fructus Capsici selfing individual plant, selects the knotting lopping of flower, thin white threads and pack into bennet, parcel thin white threads and flower and clutch, gather in the crops five steps of selfing fruit, reach quickly to obtain the purpose of Folium Styracis Suberifoliae Fructus Capsici selfing fruit. Five described step concrete operations are as follows:
1. selected individual plant: be selected in and need the Folium Styracis Suberifoliae Fructus Capsici individual plant of selfing and carry out listing mark;
2. flower is selected: in selected labeled individual plant, be about to open flower, based on the flower to the female bucket of green pepper or four;
3. thin white threads knotting lopping pack into bennet: adopting dual unijunction method by domestic white fine rule formation of knots, the coil of one end is enclosed within bennet, tightens up slightly, does not fall off with coil and is advisable, and the other end leaves the fine rule of 2cm length;
4. parcel thin white threads and flower clutching: wrap with the flower of the sticky label paper fine rule by 2cm length and Folium Styracis Suberifoliae Fructus Capsici, is advisable to two circles wrapping up a circle half, and label paper is clutched by the upper and lower side at flower, in case Apis and other insecticide enter;
5. results selfing fruit: physiological maturity results bennet hangs with the selfing fruit of white line circle labelling.
Beneficial effects of the present invention: one is artificial and consuming time few. Early stage has only to buy sticky label paper, without buying dissecting needle or syringe needle, expends 10s every time; Two is that cost is extremely cheap. The cost of sticky label paper is low, and 10 expenses spent are about 5 points of; Comparing with drop, fine rule price is only its 1/20-1/50. Three is that labelling is accurate, short and sweet. Unsuccessful if pollinating, flower can drop by nature, and white coil also can drop therewith, and successful flower of pollinating is because bennet place hangs with white coil, it is easy to distinguishes.
Detailed description of the invention:
Below in conjunction with specific embodiment, the present invention is conducted further description;
Embodiment:
1) select fine individual plant: in florescence, select excellent Folium Styracis Suberifoliae Fructus Capsici individual plant according to breeding objective, and hang up red festoons at its place of Shi Huajiewei main split and mould board and be marked;
2) flower is selected: in selected labeled individual plant, be about to open 4 four female bucket flower;
3) thin white threads knotting lopping pack into bennet: adopting dual unijunction method by domestic white fine rule formation of knots, the coil of one end is enclosed within bennet, tightens up slightly, does not fall off with coil and is advisable, and the other end leaves the fine rule of 2cm length;
4) wrap up thin white threads and flower and clutch: with the sticky label paper of long 2.9cm, wide 2cm, the fine rule of 2cm length and the flower of Folium Styracis Suberifoliae Fructus Capsici being wrapped, wrap up in two circles, and the upper and lower side of label paper is clutched;
5) results selfing fruit: physiological maturity results hang with Folium Styracis Suberifoliae Fructus Capsici fruit 3-4 of white coil, and the time is about 50d to 70 day, and success rate reaches 75-100%.
Claims (1)
1. a fine rule double-junction Folium Styracis Suberifoliae Fructus Capsici selfing technology, it is characterized in that, described method includes selected Folium Styracis Suberifoliae Fructus Capsici selfing individual plant, selects the knotting lopping of flower, thin white threads and pack into bennet, parcel thin white threads and flower and clutch, gather in the crops five steps of selfing fruit, reach quickly to obtain the purpose of Folium Styracis Suberifoliae Fructus Capsici selfing fruit; Five described step concrete operations are as follows:
1. selected individual plant: be selected in and need the Folium Styracis Suberifoliae Fructus Capsici individual plant of selfing and carry out listing mark;
2. flower is selected: in selected labeled individual plant, be about to open flower, based on the flower to the female bucket of green pepper or four;
3. thin white threads knotting lopping pack into bennet: adopting dual unijunction method by domestic white fine rule formation of knots, the coil of one end is enclosed within bennet, tightens up slightly, does not fall off with coil and is advisable, and the other end leaves the fine rule of 2cm length;
4. parcel thin white threads and flower clutching: wrap with the flower of the sticky label paper fine rule by 2cm length and Folium Styracis Suberifoliae Fructus Capsici, is advisable to two circles wrapping up a circle half, and label paper is clutched by the upper and lower side at flower, in case Apis and other insecticide enter;
5. results selfing fruit: physiological maturity results bennet hangs with the selfing fruit of white line circle labelling.
Priority Applications (1)
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CN201610067609.XA CN105660368A (en) | 2016-02-01 | 2016-02-01 | Thread dual-knot type selfing technology for red sweet pepper |
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CN201610067609.XA CN105660368A (en) | 2016-02-01 | 2016-02-01 | Thread dual-knot type selfing technology for red sweet pepper |
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CN201610067609.XA Pending CN105660368A (en) | 2016-02-01 | 2016-02-01 | Thread dual-knot type selfing technology for red sweet pepper |
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Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103444509A (en) * | 2013-07-16 | 2013-12-18 | 沈素香 | Single flower selfing isolation method for small chili |
CN104115742A (en) * | 2014-07-30 | 2014-10-29 | 胡能兵 | Thread-guiding-type pepper selfing fruit marking method |
-
2016
- 2016-02-01 CN CN201610067609.XA patent/CN105660368A/en active Pending
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103444509A (en) * | 2013-07-16 | 2013-12-18 | 沈素香 | Single flower selfing isolation method for small chili |
CN104115742A (en) * | 2014-07-30 | 2014-10-29 | 胡能兵 | Thread-guiding-type pepper selfing fruit marking method |
Non-Patent Citations (4)
Title |
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(蔬菜采种技术)编写组: "《蔬菜采种技术》", 31 December 1991, 天津科学技术出版社 * |
刘金兵等: ""42个甜(辣)椒自交系恢复基因的分布及亲缘关系的RAPD分析"", 《南京农业大学学报》 * |
戈加欣等: ""双绿5号蚕豆单花强制自交提纯技术研究"", 《种子生产》 * |
方丽等: ""辣椒杂交制种技术"", 《农业科技与信息》 * |
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