CN103827290A - 多亚基蛋白质例如抗体在转化的微生物例如巴斯德毕赤酵母中的高纯度产生 - Google Patents

多亚基蛋白质例如抗体在转化的微生物例如巴斯德毕赤酵母中的高纯度产生 Download PDF

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CN103827290A
CN103827290A CN201280035868.4A CN201280035868A CN103827290A CN 103827290 A CN103827290 A CN 103827290A CN 201280035868 A CN201280035868 A CN 201280035868A CN 103827290 A CN103827290 A CN 103827290A
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seq
ngf
antibody
approximately
pain
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P·D·麦克尼尔
N·詹森
G·L·莱斯尼基
P·齐
J·A·莱瑟姆
L·F·加西亚-马丁内斯
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Alderbio Holdings LLC
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C12N1/18—Baker's yeast; Brewer's yeast
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature

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  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
CN201280035868.4A 2011-05-20 2012-05-08 多亚基蛋白质例如抗体在转化的微生物例如巴斯德毕赤酵母中的高纯度产生 Pending CN103827290A (zh)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201910566412.4A CN110408644A (zh) 2011-05-20 2012-05-08 多亚基蛋白质例如抗体在转化的微生物例如巴斯德毕赤酵母中的高纯度产生

Applications Claiming Priority (9)

Application Number Priority Date Filing Date Title
US201161488660P 2011-05-20 2011-05-20
US61/488,660 2011-05-20
US201161496860P 2011-06-14 2011-06-14
US201161496873P 2011-06-14 2011-06-14
US61/496,873 2011-06-14
US61/496,860 2011-06-14
US201161525307P 2011-08-19 2011-08-19
US61/525,307 2011-08-19
PCT/US2012/036953 WO2012161956A2 (en) 2011-05-20 2012-05-08 High-purity production of multi-subunit proteins such as antibodies in transformed microbes such as pichia pastoris

Related Child Applications (1)

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CN201910566412.4A Division CN110408644A (zh) 2011-05-20 2012-05-08 多亚基蛋白质例如抗体在转化的微生物例如巴斯德毕赤酵母中的高纯度产生

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CN103827290A true CN103827290A (zh) 2014-05-28

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CN201910566412.4A Pending CN110408644A (zh) 2011-05-20 2012-05-08 多亚基蛋白质例如抗体在转化的微生物例如巴斯德毕赤酵母中的高纯度产生

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BR (1) BR112013029902A2 (enExample)
CA (1) CA2837000C (enExample)
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Cited By (1)

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CN108913732A (zh) * 2018-07-31 2018-11-30 华东理工大学 一种莫纳可林j异源生产的方法及应用

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AU2015230933B2 (en) * 2014-03-21 2020-08-13 Teva Pharmaceuticals International Gmbh Antagonist antibodies directed against calcitonin gene-related peptide and methods using same
TW202140776A (zh) 2016-04-26 2021-11-01 美商美國泰福生技股份有限公司 細胞培養基
KR20220031944A (ko) 2016-09-23 2022-03-14 테바 파마슈티컬스 인터내셔널 게엠베하 불응성 편두통의 치료
GB201803832D0 (en) * 2018-03-09 2018-04-25 Syndermix Ag Methods and compositions for lectin production

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WO2000056903A2 (en) * 1999-03-22 2000-09-28 Zymogenetics, Inc. IMPROVED METHODS FOR PRODUCING PROTEINS IN TRANSFORMED $i(PICHIA)
WO2002048382A2 (en) * 2000-12-13 2002-06-20 Unilever N.V. Method for the production of a heterologous protein by a fungus
WO2008063302A2 (en) * 2006-10-10 2008-05-29 Keck Graduate Institute Novel p. pastoris pastoris promoters, and the use thereof to direct expression of proteins in yeast, preferably using a haploid mating strategy

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ATE306930T1 (de) 1994-08-12 2005-11-15 Immunomedics Inc Für b-zell-lymphom und leukämiezellen spezifische immunkonjugate und humane antikörper
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WO2002048382A2 (en) * 2000-12-13 2002-06-20 Unilever N.V. Method for the production of a heterologous protein by a fungus
WO2008063302A2 (en) * 2006-10-10 2008-05-29 Keck Graduate Institute Novel p. pastoris pastoris promoters, and the use thereof to direct expression of proteins in yeast, preferably using a haploid mating strategy

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108913732A (zh) * 2018-07-31 2018-11-30 华东理工大学 一种莫纳可林j异源生产的方法及应用
CN108913732B (zh) * 2018-07-31 2022-02-08 华东理工大学 一种莫纳可林j异源生产的方法及应用

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DK2710114T3 (da) 2021-12-06
CA2837000C (en) 2021-09-14
KR20140033445A (ko) 2014-03-18
NZ618635A (en) 2016-09-30
NZ718187A (en) 2017-10-27
IL229430B (en) 2020-07-30
SMT202100677T1 (it) 2022-01-10
EA028913B1 (ru) 2018-01-31
WO2012161956A2 (en) 2012-11-29
PT2710114T (pt) 2021-12-09
SI2710114T1 (sl) 2022-01-31
SG194937A1 (en) 2013-12-30
IL229430A0 (en) 2014-01-30
MX368546B (es) 2019-10-07
EP2710114B1 (en) 2021-11-03
EA201301294A1 (ru) 2014-05-30
JP2014517698A (ja) 2014-07-24
LT2710114T (lt) 2021-12-10
WO2012161956A9 (en) 2013-09-06
MX2013013532A (es) 2015-08-20
EA028913B9 (ru) 2018-03-30
RS62628B1 (sr) 2021-12-31
KR101981662B1 (ko) 2019-05-23
HRP20211873T1 (hr) 2022-03-04
CY1124796T1 (el) 2022-11-25
BR112013029902A2 (pt) 2016-12-20
PL2710114T3 (pl) 2022-01-17
EP2710114A4 (en) 2014-11-19
WO2012161956A3 (en) 2013-01-17
HUE060418T2 (hu) 2023-02-28
JP6193220B2 (ja) 2017-09-06
ES2899381T3 (es) 2022-03-11
EP2710114A2 (en) 2014-03-26
CN110408644A (zh) 2019-11-05
CA2837000A1 (en) 2012-11-29
MX2019012022A (es) 2019-11-18
JP2018007656A (ja) 2018-01-18
JP6491696B2 (ja) 2019-03-27

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Application publication date: 20140528