CN103805552A - 一株生物合成稀少糖的谷氨酸棒杆菌工程菌株及其构建方法和应用 - Google Patents
一株生物合成稀少糖的谷氨酸棒杆菌工程菌株及其构建方法和应用 Download PDFInfo
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- CN103805552A CN103805552A CN201410055300.XA CN201410055300A CN103805552A CN 103805552 A CN103805552 A CN 103805552A CN 201410055300 A CN201410055300 A CN 201410055300A CN 103805552 A CN103805552 A CN 103805552A
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Cited By (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107746856A (zh) * | 2017-10-19 | 2018-03-02 | 中国科学院天津工业生物技术研究所 | 生产l‑稀少糖的谷氨酸棒杆菌重组菌株的构建方法与应用 |
CN108795962A (zh) * | 2018-06-25 | 2018-11-13 | 嘉兴欣贝莱生物科技有限公司 | 一种在大肠杆菌中利用果糖合成d-塔格糖的方法 |
WO2019144944A1 (zh) * | 2018-01-25 | 2019-08-01 | 中国科学院天津工业生物技术研究所 | 一种生产阿洛酮糖及其衍生物的工程菌株及其构建方法和应用 |
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CN113403239A (zh) * | 2021-08-23 | 2021-09-17 | 中国科学院天津工业生物技术研究所 | 一株谷氨酸棒杆菌株及其应用 |
CN115216411A (zh) * | 2016-10-06 | 2022-10-21 | Cj第一制糖株式会社 | 含有阿洛酮糖的细菌、真菌和酵母生长抑制剂 |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101418277A (zh) * | 2008-11-27 | 2009-04-29 | 中国药科大学 | 谷氨酸基因工程高产菌及其应用 |
EP1261718B1 (en) * | 2000-03-09 | 2010-11-03 | Evonik Degussa GmbH | Corynebacterium glutamicum genes encoding metabolic pathway proteins |
CN103509747A (zh) * | 2013-03-13 | 2014-01-15 | 江南大学 | 一种高产丁二酸的谷氨酸棒杆菌工程菌及其构建方法 |
-
2014
- 2014-02-19 CN CN201410055300.XA patent/CN103805552B/zh active Active
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP1261718B1 (en) * | 2000-03-09 | 2010-11-03 | Evonik Degussa GmbH | Corynebacterium glutamicum genes encoding metabolic pathway proteins |
CN101418277A (zh) * | 2008-11-27 | 2009-04-29 | 中国药科大学 | 谷氨酸基因工程高产菌及其应用 |
CN103509747A (zh) * | 2013-03-13 | 2014-01-15 | 江南大学 | 一种高产丁二酸的谷氨酸棒杆菌工程菌及其构建方法 |
Non-Patent Citations (3)
Title |
---|
刘平: "谷氨酸棒状杆菌磷酸解酮酶基因工程菌的构建及应用", 《西南大学硕士学位论文》, 21 August 2009 (2009-08-21) * |
谭延振: "谷氨酸棒杆菌基因敲除系统的构建", 《中国优秀硕士学位论文全文数据库(工程科技I辑)》, 15 July 2012 (2012-07-15) * |
门燕 等: "生物转化生产D-塔格糖的食品级菌种选育及L-阿拉伯糖异构酶的克隆表达", 《生物工程学报》, vol. 28, no. 5, 25 May 2012 (2012-05-25), pages 592 - 601 * |
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