CN103764837A - 用于生产具有修饰的o-糖基化的蛋白的酵母菌株 - Google Patents

用于生产具有修饰的o-糖基化的蛋白的酵母菌株 Download PDF

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Publication number
CN103764837A
CN103764837A CN201280011080.XA CN201280011080A CN103764837A CN 103764837 A CN103764837 A CN 103764837A CN 201280011080 A CN201280011080 A CN 201280011080A CN 103764837 A CN103764837 A CN 103764837A
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China
Prior art keywords
sequence
seq
coding
pomgnt1
glcnac
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CN201280011080.XA
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English (en)
Chinese (zh)
Inventor
P.博布罗维奇
W.J.库克
S.哈米尔顿
J.內特
T.A.施塔黑姆
S.维尔德特
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Merck Sharp and Dohme LLC
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Schering Corp
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C12N1/18—Baker's yeast; Brewer's yeast
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins
CN201280011080.XA 2011-02-25 2012-02-20 用于生产具有修饰的o-糖基化的蛋白的酵母菌株 Pending CN103764837A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201161446846P 2011-02-25 2011-02-25
US61/446846 2011-02-25
PCT/US2012/025809 WO2012115903A2 (fr) 2011-02-25 2012-02-20 Souche de levure pour la production de protéines avec une o-glycosylation modifiée

Publications (1)

Publication Number Publication Date
CN103764837A true CN103764837A (zh) 2014-04-30

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CN201280011080.XA Pending CN103764837A (zh) 2011-02-25 2012-02-20 用于生产具有修饰的o-糖基化的蛋白的酵母菌株

Country Status (8)

Country Link
US (1) US8936918B2 (fr)
EP (1) EP2678440B1 (fr)
JP (1) JP2014518608A (fr)
KR (1) KR20140114278A (fr)
CN (1) CN103764837A (fr)
AU (1) AU2012220890A1 (fr)
CA (1) CA2827732A1 (fr)
WO (1) WO2012115903A2 (fr)

Cited By (1)

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Publication number Priority date Publication date Assignee Title
CN113549560A (zh) * 2020-04-24 2021-10-26 中国人民解放军军事科学院军事医学研究院 一种用于糖蛋白制备的工程化酵母构建方法及其菌株

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KR102111078B1 (ko) 2013-07-04 2020-05-18 노파르티스 아게 O-만노실트랜스퍼라제 결핍 사상균 세포 및 이의 이용 방법
EP2975050B1 (fr) * 2014-07-18 2019-05-29 Sandoz Ag Quantification de TNFR2:fc mal replié
CN108064266A (zh) 2014-07-21 2018-05-22 格利科斯芬兰公司 在丝状真菌中具有哺乳动物样n-聚糖的糖蛋白的制备
CA3226465A1 (fr) * 2021-07-23 2023-01-26 Logan HURST Compositions de proteines et leurs procedes de production

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US20100331192A1 (en) * 2008-03-03 2010-12-30 Dongxing Zha Surface display of recombinant proteins in lower eukaryotes

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US7198921B2 (en) 2000-05-17 2007-04-03 Mitsubishi Pharma Corporation Process for producing protein with reduction of acidic sugar chain and glycoprotein produced thereby
US20050260729A1 (en) 2004-03-17 2005-11-24 Hamilton Stephen R Method of engineering a cytidine monophosphate-sialic acid synthetic pathway in fungi and yeast
US7449308B2 (en) * 2000-06-28 2008-11-11 Glycofi, Inc. Combinatorial DNA library for producing modified N-glycans in lower eukaryotes
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US8697394B2 (en) 2000-06-28 2014-04-15 Glycofi, Inc. Production of modified glycoproteins having multiple antennary structures
ATE440959T1 (de) 2000-06-28 2009-09-15 Glycofi Inc Verfahren für die herstellung modifizierter glykoproteine
US7598055B2 (en) 2000-06-28 2009-10-06 Glycofi, Inc. N-acetylglucosaminyltransferase III expression in lower eukaryotes
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GB0021421D0 (en) 2000-08-31 2000-10-18 Oxford Glycosciences Uk Ltd Compounds
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US8008252B2 (en) * 2001-10-10 2011-08-30 Novo Nordisk A/S Factor VII: remodeling and glycoconjugation of Factor VII
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WO2010099153A2 (fr) * 2009-02-25 2010-09-02 Merck Sharp & Dohme Corp. Ingénierie métabolique d'une voie d'assimilation du galactose dans la levure glycosynthétisée pichia pastoris
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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113549560A (zh) * 2020-04-24 2021-10-26 中国人民解放军军事科学院军事医学研究院 一种用于糖蛋白制备的工程化酵母构建方法及其菌株
WO2021213489A1 (fr) * 2020-04-24 2021-10-28 中国人民解放军军事科学院军事医学研究院 Procédé de construction d'une levure modifiée pour préparation de glycoprotéine et souche associée
CN113549560B (zh) * 2020-04-24 2024-02-13 中国人民解放军军事科学院军事医学研究院 一种用于糖蛋白制备的工程化酵母构建方法及其菌株

Also Published As

Publication number Publication date
AU2012220890A1 (en) 2013-08-29
CA2827732A1 (fr) 2012-08-30
WO2012115903A3 (fr) 2014-04-24
KR20140114278A (ko) 2014-09-26
EP2678440B1 (fr) 2018-05-23
US8936918B2 (en) 2015-01-20
EP2678440A2 (fr) 2014-01-01
WO2012115903A2 (fr) 2012-08-30
US20130295608A1 (en) 2013-11-07
JP2014518608A (ja) 2014-08-07
EP2678440A4 (fr) 2015-03-11

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Application publication date: 20140430