CN103764821B - dsRNA内切核糖核酸酶 - Google Patents

dsRNA内切核糖核酸酶 Download PDF

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Publication number
CN103764821B
CN103764821B CN201280027648.7A CN201280027648A CN103764821B CN 103764821 B CN103764821 B CN 103764821B CN 201280027648 A CN201280027648 A CN 201280027648A CN 103764821 B CN103764821 B CN 103764821B
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dsrna
endoribonuclease
sequence
seq
substrate
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Expired - Fee Related
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CN201280027648.7A
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Chinese (zh)
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CN103764821A (zh
Inventor
J.M.布尼基
K.J.斯科罗内克
D.皮安卡
A.A.苏勒
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MIEDZYNARODOWY INST BIOLOG MOLEKULARNEJ I KOMORKOWEJ
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MIEDZYNARODOWY INST BIOLOG MOLEKULARNEJ I KOMORKOWEJ
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/26—Endoribonucleases producing 5'-phosphomonoesters (3.1.26)
    • C12Y301/26003—Ribonuclease III (3.1.26.3)

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CN201280027648.7A 2011-06-08 2012-06-07 dsRNA内切核糖核酸酶 Expired - Fee Related CN103764821B (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201161494574P 2011-06-08 2011-06-08
PLP.395178 2011-06-08
US61/494574 2011-06-08
PL395178A PL222511B1 (pl) 2011-06-08 2011-06-08 Endorybonukleazy dsRNA
PCT/PL2012/050020 WO2012169917A1 (en) 2011-06-08 2012-06-07 Dsrna endoribonucleases

Publications (2)

Publication Number Publication Date
CN103764821A CN103764821A (zh) 2014-04-30
CN103764821B true CN103764821B (zh) 2016-04-27

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US (1) US9238805B2 (enExample)
EP (1) EP2718431B1 (enExample)
JP (1) JP6075892B2 (enExample)
KR (1) KR20140045371A (enExample)
CN (1) CN103764821B (enExample)
AU (1) AU2012267271B2 (enExample)
CA (1) CA2837143C (enExample)
DK (1) DK2718431T3 (enExample)
ES (1) ES2555661T3 (enExample)
PL (1) PL222511B1 (enExample)
WO (1) WO2012169917A1 (enExample)

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JP2014531908A (ja) 2011-10-14 2014-12-04 プレジデント アンド フェローズ オブ ハーバード カレッジ 構造アッセンブリによる配列決定
EP4249605B1 (en) 2011-12-22 2024-08-28 President And Fellows Of Harvard College Methods for analyte detection
ES2991004T3 (es) 2011-12-22 2024-12-02 Harvard College Métodos para la detección de analitos
WO2013184754A2 (en) 2012-06-05 2013-12-12 President And Fellows Of Harvard College Spatial sequencing of nucleic acids using dna origami probes
EP2971184B1 (en) 2013-03-12 2019-04-17 President and Fellows of Harvard College Method of generating a three-dimensional nucleic acid containing matrix
EP3003392B1 (en) 2013-06-04 2019-10-23 President and Fellows of Harvard College Rna-guideded transcriptional regulation
WO2016007839A1 (en) 2014-07-11 2016-01-14 President And Fellows Of Harvard College Methods for high-throughput labelling and detection of biological features in situ using microscopy
CN104306989A (zh) * 2014-09-27 2015-01-28 郭和友 一种利用切割酶治疗细菌方法
JP6882282B2 (ja) 2015-11-03 2021-06-02 プレジデント アンド フェローズ オブ ハーバード カレッジ 三次元核酸含有マトリックスの立体撮像のための方法と装置
PL415883A1 (pl) * 2016-01-22 2017-07-31 Międzynarodowy Instytut Biologii Molekularnej I Komórkowej RNazy MiniIII, sposoby zmiany specyficzności cięcia sekwencji RNA przez RNazy MiniIII oraz ich zastosowania
EP3449016B1 (en) 2016-04-25 2025-07-23 President and Fellows of Harvard College Hybridization chain reaction methods for in situ molecular detection
WO2018045186A1 (en) 2016-08-31 2018-03-08 President And Fellows Of Harvard College Methods of combining the detection of biomolecules into a single assay using fluorescent in situ sequencing
JP7239465B2 (ja) 2016-08-31 2023-03-14 プレジデント アンド フェローズ オブ ハーバード カレッジ 蛍光ｉｎ ｓｉｔｕ配列決定による検出のための核酸配列ライブラリの作製法
WO2018134386A1 (en) * 2017-01-23 2018-07-26 Novozymes A/S Host cells and methods for producing double-stranded rna
EP3836967A4 (en) 2018-07-30 2022-06-15 ReadCoor, LLC METHODS AND SYSTEMS FOR SAMPLE PROCESSING OR ANALYSIS
WO2020076976A1 (en) 2018-10-10 2020-04-16 Readcoor, Inc. Three-dimensional spatial molecular indexing
US20220049303A1 (en) 2020-08-17 2022-02-17 Readcoor, Llc Methods and systems for spatial mapping of genetic variants
CN120098964A (zh) * 2025-03-05 2025-06-06 中国科学院南海海洋研究所 高效热稳定的核糖核酸酶SiRe_0902在特异性识别GG位点中的应用

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JPS5459392A (en) 1977-10-14 1979-05-12 Rikagaku Kenkyusho Novel nuclease and its preparation
US20060057590A1 (en) 2004-09-14 2006-03-16 Azeddine Si-Ammour RNA probes

Non-Patent Citations (5)

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Title
Endoribonuclease RNase III is essential in Bacillus subtilis;Michelle A. Herskovitz等;《Molecular Microbiology》;20001231;第38卷(第5期);1027-1033 *
Mini-III, a fourth class of RNase III catalyses maturation of the Bacillus subtilis 23S ribosomal RNA;Gabriela Olmedo等;《Molecular Microbiology》;20081231;第68卷(第5期);1073-1076 *
Mini-III, an unusual member of the RNase III family of enzymes, catalyses 23S ribosomal RNA maturation in B. subtilis;Yulia Redko等;《Molecular Microbiology》;20080408;第68卷(第5期);1100页左栏第3段至1101页左栏第1段，图5，1103页左栏第2段至右栏第1段，图7 *
Ribosomal protein L3 bound to 23S precursor rRNAstimulates its maturation by Mini-III ribonuclease;Yulia Redko等;《Molecular Microbiology》;20090120;第71卷(第5期);1150页左栏第1段至右栏第2段 *
Structural Insight into the Mechanism of Double-Stranded RNA Processing by Ribonuclease III;Jianhua Gan等;《Cell》;20060127;第124卷;356页右栏第2-3段，图1，图3-5 *

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Publication number Publication date
AU2012267271B2 (en) 2015-06-04
WO2012169917A1 (en) 2012-12-13
PL395178A1 (pl) 2012-12-17
ES2555661T3 (es) 2016-01-07
CA2837143C (en) 2016-01-05
JP6075892B2 (ja) 2017-02-08
US20140087427A1 (en) 2014-03-27
KR20140045371A (ko) 2014-04-16
DK2718431T3 (en) 2015-11-16
AU2012267271A1 (en) 2013-12-12
EP2718431A1 (en) 2014-04-16
EP2718431B1 (en) 2015-10-28
PL222511B1 (pl) 2016-08-31
CN103764821A (zh) 2014-04-30
JP2014518641A (ja) 2014-08-07
US9238805B2 (en) 2016-01-19
CA2837143A1 (en) 2012-12-13

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