CN103764665A - 使用分选酶安装用于蛋白质连接的点击化学柄 - Google Patents

使用分选酶安装用于蛋白质连接的点击化学柄 Download PDF

Info

Publication number
CN103764665A
CN103764665A CN201280042272.7A CN201280042272A CN103764665A CN 103764665 A CN103764665 A CN 103764665A CN 201280042272 A CN201280042272 A CN 201280042272A CN 103764665 A CN103764665 A CN 103764665A
Authority
CN
China
Prior art keywords
protein
click chemistry
antibody
chemistry handle
handle
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201280042272.7A
Other languages
English (en)
Chinese (zh)
Inventor
希德·L·普莱赫
马丁·D·威特
尼古拉斯·C·约德
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Whitehead Institute for Biomedical Research
Original Assignee
Whitehead Institute for Biomedical Research
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Whitehead Institute for Biomedical Research filed Critical Whitehead Institute for Biomedical Research
Publication of CN103764665A publication Critical patent/CN103764665A/zh
Pending legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/46—Hybrid immunoglobulins
    • C07K16/468—Immunoglobulins having two or more different antigen binding sites, e.g. multifunctional antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/1072—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides by covalent attachment of residues or functional groups
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/30—Immunoglobulins specific features characterized by aspects of specificity or valency
    • C07K2317/31—Immunoglobulins specific features characterized by aspects of specificity or valency multispecific
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
    • C07K2317/569—Single domain, e.g. dAb, sdAb, VHH, VNAR or nanobody®
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
    • C07K2317/62—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising only variable region components
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/21—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a His-tag

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CN201280042272.7A 2011-06-28 2012-06-28 使用分选酶安装用于蛋白质连接的点击化学柄 Pending CN103764665A (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201161502237P 2011-06-28 2011-06-28
US61/502,237 2011-06-28
US201261624114P 2012-04-13 2012-04-13
US61/624,114 2012-04-13
PCT/US2012/044584 WO2013003555A1 (en) 2011-06-28 2012-06-28 Using sortases to install click chemistry handles for protein ligation

Publications (1)

Publication Number Publication Date
CN103764665A true CN103764665A (zh) 2014-04-30

Family

ID=47424537

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201280042272.7A Pending CN103764665A (zh) 2011-06-28 2012-06-28 使用分选酶安装用于蛋白质连接的点击化学柄

Country Status (7)

Country Link
US (2) US10081684B2 (https=)
EP (1) EP2726494B1 (https=)
JP (1) JP2014525904A (https=)
CN (1) CN103764665A (https=)
AU (1) AU2012275390A1 (https=)
CA (1) CA2840409A1 (https=)
WO (1) WO2013003555A1 (https=)

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104130308A (zh) * 2014-07-29 2014-11-05 吉林大学 一种蛋白质定点peg修饰的方法及所得peg修饰的蛋白质
CN108026560A (zh) * 2015-09-25 2018-05-11 豪夫迈·罗氏有限公司 在低共熔溶剂中使用分选酶的转酰胺反应
CN108271375A (zh) * 2015-09-25 2018-07-10 豪夫迈·罗氏有限公司 包含分选酶缀合环的重组免疫球蛋白重链及其缀合物
CN109072212A (zh) * 2016-03-30 2018-12-21 豪夫迈·罗氏有限公司 改善的分选酶
WO2020151762A1 (zh) * 2019-01-25 2020-07-30 信达生物制药(苏州)有限公司 新型双特异性抗体分子以及同时结合pd-l1和lag-3的双特异性抗体
WO2020181756A1 (zh) * 2019-03-13 2020-09-17 南开大学 基于α螺旋把手提高蛋白核酸生物耦合效率的方法
US11732044B2 (en) 2017-12-27 2023-08-22 Innovent Biologics (Suzhou) Co., Ltd. Anti-LAG-3 antibody and use thereof
CN117255810A (zh) * 2021-03-05 2023-12-19 杨百翰大学 用于使用分子内到分子间构象转换控制蛋白质二聚化的方法

Families Citing this family (94)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SG175077A1 (en) 2009-04-07 2011-11-28 Roche Glycart Ag Trivalent, bispecific antibodies
US9676845B2 (en) 2009-06-16 2017-06-13 Hoffmann-La Roche, Inc. Bispecific antigen binding proteins
EP2478013B1 (en) 2009-09-16 2018-10-24 F.Hoffmann-La Roche Ag Coiled coil and/or tether containing protein complexes and uses thereof
TW201138821A (en) 2010-03-26 2011-11-16 Roche Glycart Ag Bispecific antibodies
CN103068846B9 (zh) 2010-08-24 2016-09-28 弗·哈夫曼-拉罗切有限公司 包含二硫键稳定性Fv片段的双特异性抗体
SG191153A1 (en) 2010-12-23 2013-07-31 Hoffmann La Roche Polypeptide-polynucleotide-complex and its use in targeted effector moiety delivery
AR085404A1 (es) 2011-02-28 2013-09-25 Hoffmann La Roche Proteinas de union a antigeno
MX342034B (es) 2011-02-28 2016-09-12 Hoffmann La Roche Proteinas monovalentes que se unen a antigenos.
AU2012275390A1 (en) 2011-06-28 2014-01-16 Whitehead Institute For Biomedical Research Using sortases to install click chemistry handles for protein ligation
WO2013065009A1 (en) * 2011-11-01 2013-05-10 National Institute Of Immunology A sortase-click reaction suite for synthesis of multivalent dendrimeric protein assembly
CA2861124A1 (en) 2012-02-10 2013-08-15 Genentech, Inc. Single-chain antibodies and other heteromultimers
US9717685B2 (en) 2012-03-26 2017-08-01 President And Fellows Of Harvard College Lipid-coated nucleic acid nanostructures of defined shape
US9751945B2 (en) 2012-04-13 2017-09-05 Whitehead Institute For Biomedical Research Sortase-modified VHH domains and uses thereof
US11339208B1 (en) 2012-05-31 2022-05-24 United States Of America As Represented By The Secretary Of The Air Force Camelidae single-domain antibodies against Yersinia pestis and methods of use
US9267127B2 (en) 2012-06-21 2016-02-23 President And Fellows Of Harvard College Evolution of bond-forming enzymes
RU2639287C2 (ru) * 2012-06-27 2017-12-20 Ф. Хоффманн-Ля Рош Аг Способ отбора и получения высокоселективных и мультиспецифичных нацеливающих групп с заданными свойствами, включающих по меньшей мере две различные связывающие группировки, и их применения
KR20150030744A (ko) 2012-06-27 2015-03-20 에프. 호프만-라 로슈 아게 표적에 특이적으로 결합하는 하나 이상의 결합 단위를 포함하는 항체 Ｆｃ－영역 접합체의 제조 방법 및 그의 용도
RU2646159C2 (ru) 2012-09-14 2018-03-01 Ф. Хоффманн-Ля Рош Аг Способ получения и отбора молекул, включающих по меньшей мере две различные группировки, и их применение
WO2014145654A1 (en) 2013-03-15 2014-09-18 The Trustees Of The University Of Pennsylvania Method for the site-specific covalent cross-linking of antibodies to surfaces
US9631218B2 (en) 2013-03-15 2017-04-25 The Trustees Of The University Of Pennsylvania Sortase-mediated protein purification and ligation
PT3611180T (pt) * 2013-03-15 2022-03-15 Biomolecular Holdings Llc Imunoglobulina híbrida que contém uma ligação não peptídica
US10077299B2 (en) * 2013-04-25 2018-09-18 Abtlas Co., Ltd. Method for refining protein including self-cutting cassette and use thereof
CN105722851A (zh) * 2013-04-28 2016-06-29 秦刚 一种新型的连接子及其制备方法和用途
PL2991683T3 (pl) 2013-05-02 2020-03-31 Glykos Finland Oy Koniugaty glikoproteiny lub glikanu z toksycznym ładunkiem
WO2014183066A2 (en) 2013-05-10 2014-11-13 Whitehead Institute For Biomedical Research Protein modification of living cells using sortase
EP2994491A4 (en) 2013-05-10 2016-12-07 Whitehead Inst Biomedical Res IN VITRO PREPARATION OF ERYTHROCYTES WITH SORTASE-PROOF PROTEINS
AU2014287071A1 (en) * 2013-07-10 2016-01-28 Children's Medical Center Corporation Compositions and methods relating to nucleic acid-protein complexes
WO2015042393A2 (en) * 2013-09-20 2015-03-26 President And Fellows Of Harvard College Evolved sortases and uses thereof
BR112016006929A2 (pt) 2013-10-11 2017-09-19 Hoffmann La Roche Anticorpo, ácido nucleico, vetor de expressão, célula hospedeira, métodos de preparação de anticorpo, de tratamento de pacientes e de geração de um anticorpo, composição farmacêutica e uso do anticorpo
US10556024B2 (en) 2013-11-13 2020-02-11 Whitehead Institute For Biomedical Research 18F labeling of proteins using sortases
CN104844711B (zh) * 2014-02-18 2018-07-06 南通表源生物技术有限公司 一种多聚体荧光蛋白复合物及其应用
KR102587838B1 (ko) 2014-03-14 2023-10-12 바이오몰레큘러 홀딩스 엘엘씨 비-펩티드 결합을 함유하는 하이브리드 면역글로불린
AU2015259533B2 (en) 2014-05-10 2019-11-28 Vivasor, Inc. Chemically-locked bispecific antibodies
US10053683B2 (en) 2014-10-03 2018-08-21 Whitehead Institute For Biomedical Research Intercellular labeling of ligand-receptor interactions
CA2964837C (en) 2014-10-29 2023-10-17 Ludwig-Maximilians-Universitaet Muenchen Means and methods for site-specific functionalization of polypeptides
EP3227332B1 (en) 2014-12-03 2019-11-06 F.Hoffmann-La Roche Ag Multispecific antibodies
CA2966287A1 (en) 2014-12-17 2016-06-23 F. Hoffmann-La Roche Ag Novel methods for enzyme mediated polypeptide conjugation using sortase
EP3234177A1 (en) 2014-12-17 2017-10-25 F. Hoffmann-La Roche AG Activity assay for bond forming enzymes
KR20170098821A (ko) 2014-12-17 2017-08-30 에프. 호프만-라 로슈 아게 소르타아제를 사용한 １ 단계로의 이중 폴리펩티드 컨쥬게이션을 위한 효소 원-포트 반응
KR101637010B1 (ko) * 2015-04-24 2016-07-07 광주과학기술원 위치 특이적으로 알부민이 연결된 요산 산화효소 및 단백질에 위치 특이적으로 알부민을 연결하는 방법
TWI687228B (zh) * 2015-05-11 2020-03-11 美商索倫多醫療公司 化學鎖固之雙特異性抗體
EP3294341A4 (en) 2015-05-12 2019-01-23 The Trustees Of The University Of Pennsylvania COMPOSITIONS AND METHOD FOR THE PRODUCTION OF ANTIBODY CONJUGATES
WO2016191200A1 (en) 2015-05-22 2016-12-01 Clemson University Conotoxin peptides for use in biofouling deterrence
JP2017043547A (ja) * 2015-08-24 2017-03-02 国立研究開発法人理化学研究所 ポリペプチドを結合した成長因子及びその利用
WO2017050866A1 (en) 2015-09-25 2017-03-30 F. Hoffmann-La Roche Ag Novel soluble sortase a
CN108138204B (zh) 2015-09-25 2021-12-31 豪夫迈·罗氏有限公司 使用分选酶a生产硫酯的方法
US12048753B2 (en) 2015-10-01 2024-07-30 Whitehead Institute For Biomedical Research Labeling of antibodies
CA3005454A1 (en) 2015-11-18 2017-05-26 Sorrento Therapeutics, Inc. Chemically-locked bispecific antibodies
CA3014921A1 (en) 2016-02-16 2017-08-24 Research Development Foundation Sortase-modified molecules and uses thereof
JP6959251B2 (ja) 2016-03-24 2021-11-02 ジェニア・テクノロジーズ・インコーポレイテッド ナノポアシステムに有用な部位特異的バイオコンジュゲーション方法および組成物
WO2017186855A1 (en) 2016-04-28 2017-11-02 Forschungsverbund Berlin E.V. Unusual substrates of tubulin tyrosine ligase
CN109789092A (zh) 2016-07-13 2019-05-21 哈佛学院院长等 抗原呈递细胞模拟支架及其制备和使用方法
EP3512564B1 (en) 2016-09-14 2024-01-17 The Trustees of The University of Pennsylvania Proximity-based sortase-mediated protein purification and ligation
WO2018053634A1 (en) * 2016-09-21 2018-03-29 The Governing Council Of The University Of Toronto Hemoglobin based oxygen carrier and method of preparation
US10738338B2 (en) 2016-10-18 2020-08-11 The Research Foundation for the State University Method and composition for biocatalytic protein-oligonucleotide conjugation and protein-oligonucleotide conjugate
EP4649966A3 (en) 2016-12-01 2026-02-18 Regeneron Pharmaceuticals, Inc. Radiolabeled anti-pd-l1 antibodies for immuno-pet imaging
US11951165B2 (en) 2016-12-30 2024-04-09 Vaxcyte, Inc. Conjugated vaccine carrier proteins
CN110366428B (zh) 2016-12-30 2024-05-17 Vaxcyte公司 具有非天然氨基酸的多肽-抗原缀合物
EP3577138A1 (en) 2017-02-06 2019-12-11 Innate Pharma Immunomodulatory antibody drug conjugates binding to a human mica polypeptide
EP3596108A4 (en) 2017-03-15 2020-12-23 Pandion Operations, Inc. TARGETED IMMUNOTOLERANCE
EP3624791B1 (en) * 2017-05-17 2026-05-06 Research Foundation Of The City University Of New York Reagent for site-selective bioconjugation of proteins or antibodies
US10676516B2 (en) 2017-05-24 2020-06-09 Pandion Therapeutics, Inc. Targeted immunotolerance
JP2020524483A (ja) 2017-05-25 2020-08-20 プレリス バイオロジクス，インク． 三次元印刷された器官、デバイス、およびマトリックス
DE102017115522B4 (de) * 2017-07-11 2019-11-14 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Verfahren zur Herstellung von Block-Polymeren mittels Verknüpfung von Blöcken durch eine Transpeptidase und Block-Polymere erhalten durch Transpeptidase-Verknüpfung
USRE50550E1 (en) 2017-12-06 2025-08-26 Pandion Operations, Inc. IL-2 muteins and uses thereof
US10946068B2 (en) 2017-12-06 2021-03-16 Pandion Operations, Inc. IL-2 muteins and uses thereof
US10174092B1 (en) 2017-12-06 2019-01-08 Pandion Therapeutics, Inc. IL-2 muteins
BR112020012099A2 (pt) * 2017-12-18 2020-11-17 Janssen Biotech, Inc. radiomarcação de polipeptídeos
WO2019129053A1 (zh) * 2017-12-26 2019-07-04 南京金斯瑞生物科技有限公司 以抗体Fc区为骨架的融合蛋白二聚体及其应用
US12054560B2 (en) * 2017-12-29 2024-08-06 Jiangnan University Bifunctional molecules recruiting antibodies and targeting cancer cells
WO2019143631A1 (en) * 2018-01-16 2019-07-25 Phoenix Biomolecular Engineering Foundation Universal viral vaccines
US11866482B2 (en) 2018-02-07 2024-01-09 Duke University System and method for homogenous GPCR phosphorylation and identification of beta-2 adrenergic receptor positive allosteric modulators
GB201900621D0 (en) * 2019-01-16 2019-03-06 Ipsen Biopharm Ltd Labelled polypeptides
US12268748B2 (en) 2019-04-10 2025-04-08 President And Fellows Of Harvard College Nucleic acid nanostructures crosslinked with oligolysine
BR112021023345A2 (pt) 2019-05-20 2022-02-01 Pandion Operations Inc Imunotolerância com alvo em madcam
WO2020247724A1 (en) 2019-06-07 2020-12-10 Dana-Farber Cancer Institute, Inc. Dna nanostructure-based vaccines
WO2020252455A1 (en) 2019-06-13 2020-12-17 The General Hospital Corporation Engineered human-endogenous virus-like particles and methods of use thereof for delivery to cells
WO2021141924A1 (en) 2020-01-07 2021-07-15 Encodia, Inc. Methods for stable complex formation and related kits
EP4107187A4 (en) 2020-02-21 2024-07-03 Pandion Operations, Inc. TISSUE-TARGETED IMMUNOTOLERANCE WITH A CD39 EFFECTOR
EP4157883A4 (en) * 2020-06-02 2024-12-04 Children's Medical Center Corporation NANOBODY CONJUGATES (VHH) AND THEIR USES
US20230020297A1 (en) * 2020-06-05 2023-01-19 Proabtech Co., Ltd. Uox-albumin conjugate with certain numbers of albumin conjugated thereto, and manufacturing method thereof
CA3189601A1 (en) 2020-07-24 2022-01-27 The General Hospital Corporation Enhanced virus-like particles and methods of use thereof for delivery to cells
EP4196581B1 (en) 2020-08-19 2026-02-25 Encodia, Inc. Sequential encoding methods and related kits
KR102917231B1 (ko) 2021-03-08 2026-01-26 진콴텀 헬스케어 (쑤저우) 씨오., 엘티디. 항체 면역 작용제 접합체 및 이의 적용
EP4448722A4 (en) * 2021-12-14 2025-12-03 Prellis Biologics Inc Three-dimensional synthetic lymphoid organs and organoids for antibody design and testing
AU2023226512A1 (en) 2022-02-23 2024-08-29 Bright Peak Therapeutics Ag Immune antigen specific il-18 immunocytokines and uses thereof
WO2025041101A1 (en) 2023-08-23 2025-02-27 Bright Peak Therapeutics Ag Activatable il-18 immunocytokines and uses thereof
WO2025041102A1 (en) 2023-08-23 2025-02-27 Bright Peak Therapeutics Ag Targeted immune activation with il-18 immunocytokines
WO2025059162A1 (en) 2023-09-11 2025-03-20 Dana-Farber Cancer Institute, Inc. Car-engager containing il-2 variants to enhance the functionality of car t cells
WO2025238253A1 (en) 2024-05-16 2025-11-20 Synaffix B.V. Antibody-oligonucleotide conjugates
WO2026008881A1 (en) 2024-07-05 2026-01-08 Synaffix B.V. Extracellular vesicles comprising biomolecule-conjugates
WO2026069134A1 (en) 2024-09-26 2026-04-02 Bright Peak Therapeutics Ag Human therapy with il-18 immunocytokines
WO2026083296A1 (en) 2024-10-16 2026-04-23 Bright Peak Therapeutics Ag Trispecific compositions comprising il-18, vegf binding domains, and pd-1 binding domains
WO2026083295A1 (en) 2024-10-16 2026-04-23 Bright Peak Therapeutics Ag Trispecific compositions comprising il-2, vegf binding domains, and pd-1 binding domains

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008148143A1 (en) * 2007-06-06 2008-12-11 University Of Wollongong Diagnostics in a monoplex/multiplex format

Family Cites Families (44)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4816567A (en) 1983-04-08 1989-03-28 Genentech, Inc. Recombinant immunoglobin preparations
US5225539A (en) 1986-03-27 1993-07-06 Medical Research Council Recombinant altered antibodies and methods of making altered antibodies
US5530101A (en) 1988-12-28 1996-06-25 Protein Design Labs, Inc. Humanized immunoglobulins
US5859205A (en) 1989-12-21 1999-01-12 Celltech Limited Humanised antibodies
US5670132A (en) 1994-09-20 1997-09-23 Immunomedics, Inc. Modified radioantibody fragments for reduced renal uptake
WO1998033809A1 (en) 1997-01-31 1998-08-06 The General Hospital Corporation Compositions and methods for imaging gene expression
ES2297889T3 (es) 1997-07-14 2008-05-01 Bolder Biotechnology, Inc. Derivados de hormona de crecimiento y proteinas relacionadas.
EP1233780A4 (en) 1999-04-15 2004-09-22 Univ California IDENTIFICATION OF THE SORTASE GENE
US7101692B2 (en) 1999-04-15 2006-09-05 The Regents Of The University Of California Identification of sortase gene
CA2413538A1 (en) 2000-07-06 2002-01-17 Bristol-Myers Squibb Pharma Company Stable radiopharmaceutical compositions and methods for preparation thereof
US7279304B2 (en) 2000-12-18 2007-10-09 President And Fellows Of Harvard College Methods for preparation of macrocyclic molecules and macrocyclic molecules prepared thereby
AU2002364586A1 (en) 2001-12-21 2003-07-30 Delta Biotechnology Limited Albumin fusion proteins
DE60332358D1 (de) 2002-09-09 2010-06-10 Hanall Pharmaceutical Co Ltd Protease-resistente modifizierte interferon alpha polypeptide
CN101899114A (zh) 2002-12-23 2010-12-01 惠氏公司 抗pd-1抗体及其用途
US6960473B2 (en) 2003-02-27 2005-11-01 Istituto Superiore Di Sanita In vitro mass production of human erythroid cells from the blood of normal donors and thalassemic patients
DE10335584B4 (de) 2003-07-31 2006-06-29 Philipps-Universität Marburg Verfahren zur Herstellung zyklischer Moleküle
WO2005051976A2 (en) 2003-11-20 2005-06-09 Ansata Therapeutics, Inc. Protein and peptide ligation processes and one-step purification processes
CN101115769A (zh) 2004-05-19 2008-01-30 马克西根公司 干扰素-α多肽和偶联物
EP1753859B1 (en) 2004-06-04 2014-07-30 Université Pierre et Marie Curie (Paris 6) A method for producing red blood cells
US7524831B2 (en) 2005-03-02 2009-04-28 Schering Corporation Treatments for Flaviviridae virus infection
BR122017025062B8 (pt) 2007-06-18 2021-07-27 Merck Sharp & Dohme anticorpo monoclonal ou fragmento de anticorpo para o receptor de morte programada humano pd-1, polinucleotídeo e composição compreendendo o referido anticorpo ou fragmento
JP2009189325A (ja) * 2008-02-15 2009-08-27 Kobe Univ 第１級アミンを有する化合物をタンパク質の特定の部位に結合させる方法
US8211656B2 (en) 2008-08-13 2012-07-03 The Invention Science Fund I, Llc Biological targeting compositions and methods of using the same
AU2009288730B2 (en) 2008-08-25 2013-06-20 Amplimmune, Inc. Compositions of PD-1 antagonists and methods of use
JP2012500855A (ja) 2008-08-25 2012-01-12 アンプリミューン、インコーポレーテッド Ｐｄ−１アンタゴニストおよび感染性疾患を処置するための方法
WO2010033913A1 (en) 2008-09-22 2010-03-25 Icb International, Inc. Antibodies, analogs and uses thereof
WO2010078376A2 (en) 2008-12-30 2010-07-08 Ventana Medical Systems, Inc. Fc-specific polymer-conjugated antibodies and their diagnostic use
WO2010087994A2 (en) 2009-01-30 2010-08-05 Whitehead Institute For Biomedical Research Methods for ligation and uses thereof
JP5844159B2 (ja) 2009-02-09 2016-01-13 ユニヴェルシテ デクス−マルセイユＵｎｉｖｅｒｓｉｔｅ Ｄ’Ａｉｘ−Ｍａｒｓｅｉｌｌｅ Ｐｄ−１抗体およびｐｄ−ｌ１抗体ならびにその使用
KR20130009760A (ko) 2010-02-10 2013-01-23 이뮤노젠 아이엔씨 Ｃｄ２０ 항체 및 이의 용도
PL2539441T3 (pl) 2010-02-22 2017-06-30 Université Pierre Et Marie Curie (Paris 6) Podłoże do hodowli komórkowej do wzrostu i różnicowania komórek linii hematopoetycznych
EP2545078A1 (en) 2010-03-11 2013-01-16 UCB Pharma, S.A. Pd-1 antibody
WO2011133704A2 (en) 2010-04-20 2011-10-27 Whitehead Institute For Biomedical Researh Modified polypeptides and proteins and uses thereof
WO2012142659A1 (en) 2011-04-19 2012-10-26 Baker Idi Heart And Diabetes Institute Holdings Limited Site-selective modification of proteins
AU2012275390A1 (en) 2011-06-28 2014-01-16 Whitehead Institute For Biomedical Research Using sortases to install click chemistry handles for protein ligation
WO2013132317A1 (en) 2012-03-07 2013-09-12 Aurigene Discovery Technologies Limited Peptidomimetic compounds as immunomodulators
US9751945B2 (en) 2012-04-13 2017-09-05 Whitehead Institute For Biomedical Research Sortase-modified VHH domains and uses thereof
US20140030697A1 (en) 2012-06-14 2014-01-30 Massachusetts Institute Of Technology Sortase-mediated modification of viral surface proteins
US9267127B2 (en) 2012-06-21 2016-02-23 President And Fellows Of Harvard College Evolution of bond-forming enzymes
EP2994491A4 (en) 2013-05-10 2016-12-07 Whitehead Inst Biomedical Res IN VITRO PREPARATION OF ERYTHROCYTES WITH SORTASE-PROOF PROTEINS
WO2014183066A2 (en) 2013-05-10 2014-11-13 Whitehead Institute For Biomedical Research Protein modification of living cells using sortase
US9878045B2 (en) 2013-10-15 2018-01-30 Regents Of The University Of Minnesota Triorthogonal reagents for dual protein conjugation
US10556024B2 (en) 2013-11-13 2020-02-11 Whitehead Institute For Biomedical Research 18F labeling of proteins using sortases
US10053683B2 (en) 2014-10-03 2018-08-21 Whitehead Institute For Biomedical Research Intercellular labeling of ligand-receptor interactions

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008148143A1 (en) * 2007-06-06 2008-12-11 University Of Wollongong Diagnostics in a monoplex/multiplex format

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
JOHN M.ANTOS等: "Site-specific N- and C- terminal labeling of a single polypeptide using sortases of different specificity", 《 J.AM.CHEM.SOC.》 *
JUNPENG XIAO 等: "Synthesis of N-terminally linked protein dimmers and trimers by a combinded native chemical ligation-CuAAC click chemistry strategy", 《ORGANIC LETTERS》 *
LEVARY D.A.等: "Protein-Protein Fusion Catalyzed by Sortase A", 《PLOS ONE》 *
SLETTEN EM等: "Bioorthogonal chemistry: fishing for selectivity in a sea of functionally", 《ANGEW CHEM INT ED ENGL. 》 *
WITTE M.D.等: "Production of unnaturally linked chimeric proteins using a combination of sortase-catalyzed transpeptidation and click chemistry", 《NATURE PROTOCOLS》 *

Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104130308A (zh) * 2014-07-29 2014-11-05 吉林大学 一种蛋白质定点peg修饰的方法及所得peg修饰的蛋白质
CN108026560A (zh) * 2015-09-25 2018-05-11 豪夫迈·罗氏有限公司 在低共熔溶剂中使用分选酶的转酰胺反应
CN108271375A (zh) * 2015-09-25 2018-07-10 豪夫迈·罗氏有限公司 包含分选酶缀合环的重组免疫球蛋白重链及其缀合物
CN109072212A (zh) * 2016-03-30 2018-12-21 豪夫迈·罗氏有限公司 改善的分选酶
CN109072212B (zh) * 2016-03-30 2022-10-04 豪夫迈·罗氏有限公司 改善的分选酶
US11732044B2 (en) 2017-12-27 2023-08-22 Innovent Biologics (Suzhou) Co., Ltd. Anti-LAG-3 antibody and use thereof
WO2020151762A1 (zh) * 2019-01-25 2020-07-30 信达生物制药(苏州)有限公司 新型双特异性抗体分子以及同时结合pd-l1和lag-3的双特异性抗体
WO2020181756A1 (zh) * 2019-03-13 2020-09-17 南开大学 基于α螺旋把手提高蛋白核酸生物耦合效率的方法
US11505800B2 (en) 2019-03-13 2022-11-22 Nankai University Method for improving bio-coupling efficiency between protein and nucleic acid based on alpha-helix handle
CN117255810A (zh) * 2021-03-05 2023-12-19 杨百翰大学 用于使用分子内到分子间构象转换控制蛋白质二聚化的方法

Also Published As

Publication number Publication date
US20140249296A1 (en) 2014-09-04
JP2014525904A (ja) 2014-10-02
CA2840409A1 (en) 2013-01-03
EP2726494B1 (en) 2017-01-04
US10081684B2 (en) 2018-09-25
AU2012275390A1 (en) 2014-01-16
US11028185B2 (en) 2021-06-08
US20190112394A1 (en) 2019-04-18
EP2726494A4 (en) 2014-12-24
EP2726494A1 (en) 2014-05-07
WO2013003555A1 (en) 2013-01-03

Similar Documents

Publication Publication Date Title
CN103764665A (zh) 使用分选酶安装用于蛋白质连接的点击化学柄
JP2014525904A5 (https=)
US9751945B2 (en) Sortase-modified VHH domains and uses thereof
US10647765B2 (en) Amino acid sequence presenting fusion polypeptide and its use
US20210171998A1 (en) Lysine conjugated immunoglobulins
EP3353201B1 (en) Recombinant immunoglobulin heavy chains comprising a sortase conjugation loop and conjugates thereof
CN108602878A (zh) C端赖氨酸缀合免疫球蛋白
JP2016511279A (ja) 免疫リガンド／ペイロード複合体の生産方法
JP2022511718A (ja) 抗Ｔａｑ ＤＮＡポリメラーゼ抗体及びその用途
US20210317233A1 (en) Super Versatile Method for Imparting New Binding Specificity to Antibody
AU2021203496A1 (en) Super versatile method for presenting cyclic peptide motif on protein structure
AU2019266042B2 (en) Artificial protein containing an antibody antigen-binding region, fused with bioactive peptides
US10570197B2 (en) Fd chain gene or L chain gene capable of increasing secretion amount of fab-type antibody
CA2903252C (en) Ribotoxin molecules derived from sarcin and other related fungal ribotoxins
HK1197554B (en) Using sortases to install click chemistry handles for protein ligation
HK1197554A (en) Using sortases to install click chemistry handles for protein ligation
JP2021141861A (ja) 抗原に対する親和力が向上した抗体のスクリーニング方法および製造方法
NZ761205B2 (en) Highly versatile method of presenting cyclic peptide on protein structure
NZ616871B2 (en) Amino acid sequence presenting fusion polypeptide and its use

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C02 Deemed withdrawal of patent application after publication (patent law 2001)
WD01 Invention patent application deemed withdrawn after publication

Application publication date: 20140430