CN103749390A - Method for breeding sipunculus nudus parent - Google Patents

Method for breeding sipunculus nudus parent Download PDF

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CN103749390A
CN103749390A CN201210565958.6A CN201210565958A CN103749390A CN 103749390 A CN103749390 A CN 103749390A CN 201210565958 A CN201210565958 A CN 201210565958A CN 103749390 A CN103749390 A CN 103749390A
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pond
parent
water
sand
breeding
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CN103749390B (en
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邹杰
杨家林
王志成
彭慧婧
张琴
蒋艳
文雪
童潼
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GUANGXI ZHUANG AUTONOMOUS REGION INSTITUTE OF OCEANOLOGY
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GUANGXI ZHUANG AUTONOMOUS REGION INSTITUTE OF OCEANOLOGY
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Abstract

The invention discloses a method for breeding a sipunculus nudus parent. Artificial breeding of sipunculus nudus is high in cost, very instable in effect and significantly relevant to poor artificial spawning effect, high mortality and inconsistent egg laying quality of the sipunculus nudus parent. Meanwhile, mismatch of the yield is caused due to excessive concentration of egg laying in induced spawning, leading to resource waste. By adopting the measures of building of a sipunculus nudus parent breeding pond, indoor artificial food culture, maturing and breeding management, disease control and prevention and artificial control of egg laying, the mortality is lowered to 7-12 percent within 40 days of artificial indoor parent maturing and egg laying breeding, the single yield of an individual parent of 20g is 50,000-70,000 eggs, the metamosphsis rate of hatched larvae is 13-86 percent, and artificial control of egg laying can be performed according to the production capacity. By adopting the method, the source and controllability of fertilized eggs are realized in plant artificial breeding and selective breeding of the sipunculus nudus, the egg laying time of the parent is prolonged, and the breeding cost is saved.

Description

A kind of Sipunculus nudus parents culture method
Technical field
The present invention relates to a kind of indoor parents culture method of Sipunculus nudus, the cultivation of parent indoors artificial and the control that are applicable to the coastal Sipunculus nudus factorial seedling growth of southern china and fine-variety breeding are laid eggs, and belong to mariculture technology field.
Background technology
Sipunculus nudus Sipunculus nudus, has another name called Sipunculus nudus, is commonly called as sandworm, is subordinate to sipunculan door Sipunculida Genus Sipunculus, is water warm cosmopolitan, and coastal on the south China Qingdao all have a distribution.Sipunculus nudus food chain level is inferior low, strong stress resistance, and growth is fast.Sipunculus nudus belongs to regional characters synusia system, and resource colony is stable, and natural distribution density is high, and scope of activities is little, and nothing is long apart from migration transport phenomena.Enclosing in the Xing Xia pool, port of 200-300 mu, the distribution density of Sipunculus nudus reaches 15-20 bar/m 2, as can be seen here, Sipunculus nudus is very suitable for propagating artificially on a large scale.
Sipunculus nudus meat is tender and crisp, and taste delicate flavour is nutritious, is rich in the multiple nutritional components such as protein, fat, calcium, phosphorus, iron, can be used as the splendid raw material of culinary art delicious food.Sipunculus nudus market value is high, welcome by the coastal people.The coastal fisherman in south is also always using digging Sipunculus nudus as a job of supporting the family.In recent years Sipunculus nudus has been carried out to the trial of propagating artificially, the enthusiasm that people propagate artificially Sipunculus nudus continues to raise.But the seed that the basis that Sipunculus nudus is propagated artificially is Sipunculus nudus produces, Sipunculus nudus seed produces wretched insufficiency at present, artificial breeding cost is high, and nursery effect is unstable, and these problems are largely relevant with the quality of laying eggs with Sipunculus nudus parent mature condition.Sipunculus nudus parent artificial induced spawning weak effect, the quality of laying eggs is uneven, very easily breaks out disease in the cultivating process of hastening parturition, lethality 30%-100%, hastening parturition lays eggs too simultaneously concentrates and forms production capacity and do not match and cause waste.Thereby in Sipunculus nudus factorial seedling growth and two kinds of Breeding Process, need a kind of effectively artificial parents culture method, the problem occurring in process that the artitificial food that solves Sipunculus nudus parent is selected, accelerating is cultivated, disease prevention and control and Artificial Control are laid eggs.Through retrieval, for Sipunculus nudus parents culture aspect, be detected in us to parents culture initial trial Journal of Sex Research, in parent indoor culture and control, have no the disclosure of correlation technique aspect laying eggs both at home and abroad.
Summary of the invention
The object of this invention is to provide a kind of indoor parents culture method of Sipunculus nudus, the measure of comprise that the pond construction of parent indoor culture, parent are selected, the cultivation of indoors artificial bait, accelerating cultivating and managing, control and prevention of disease and Artificial Control being laid eggs, for Sipunculus nudus industrial artificial seedling rearing and fine-variety breeding provide the fertilized egg source of high-quality, realize the utilization of efficiently laying eggs of parent, save seedling cost.
For achieving the above object, the present invention has taked following technical scheme: a kind of Sipunculus nudus parents culture method, comprises the steps:
(1) indoor culture pond is set: cultivation pond is cement pit, floor space 10-15m 2dark 0.8-1.5m, at the bottom of pond, one end is provided with a discharge outlet, 1/4Wei Wusha district near discharge outlet, remaining 3/4 is parents culture layer, centre stacks and separates with brick, parents culture layer bottom paving particle diameter 3-7mm grit, thickness 4-7cm, covers one deck 30-50 eye mesh screen on grit, and encase every brick, on screen cloth, lay the sand bed of the thick 25-30cm of particle diameter 0.5-5mm, sea water advanced is 35-40cm, cultivates the sunshade net that top, pond 1m arranges retractable light transmittance 10-15%, illuminating lamp is equipped with, about 3m at the bottom of pond in sunshade net top 2place an airstone, every pond is equipped with 10-12 heating rod, and pool wall top cover layer of polyethylene film is in order to insulation;
(2) parent is selected: late May to by the end of August, is selected quality and is greater than 18g, and rhynchodaenm tentacle end is without disappearance and the undamaged individuality of body surface, and female-male proportion is 5:2-3, after choosing with seawater flushing, 28-25 bar/m 2density, be invested on the cultivation layer of cultivating pond throw something and feed diatom and seawood meal;
(3) accelerating cultivating and managing: when cultivating pond maritime interior waters temperature lower than 30 ℃, make temperature rise to 30-33 ℃ of temperature control cultivate with heating rod, original water temperature reaches 30 ℃ to be needed that water temperature is down to 30 ℃ and heat up below again, and programming rate is less than 5 ℃/5 days; Drain water every day once, the dry slow water filling in Yu Wusha district again after 4 hours, the water filling flow velocity 3-4m of revealing 3/ h, the dark 35-40cm of water filling; After draining water, throw something and feed, in throwing in the first 1 day diatom sand of throwing something and feeding of parent, every pond 2800-3200g, throws in the parent seawood meal of throwing something and feeding the same day, and then every pond 5g throws something and feeds the husky and seawood meal of diatom the next day in turn;
(4) control and prevention of disease: while selecting parent with the Benzylpenicillin sodium salt sea water solution of 0.1-0.5mg/L to parent body surface sterilization 10-15min, cultivate before throwing something and feeding in pond and use seawater flushing sand bed once, after every 7-10 day parent being taken out from sand with the chlorine-containing disinfectant sterilization parents culture layer 30min of available chlorine 100mg/L, then with in the sodium thiosulfate sea water solution of 10mg/L and residual chlorine 30min, with sand filter seawater, rinse, more again throw in parent and continue to cultivate;
(6) control and lay eggs: control water temperature at 30-33 ℃, with sunshade net shading, depth of water 0.8-1.2m, the ammonia spirit 0.2ml/m of dropping mass fraction 28% 3, after 24-28h, to lay eggs, restriction is laid eggs to adopt to reduce and is cultivated water level to exceeding the about 3cm of sand bed, cancels shading, and be continuously applied illumination with illuminating lamp night, is cooled to the measure of 1-2 ℃;
Described screen cloth is commercial goods;
Described is river sand or the sea sand through screening with sand, before using through the sunlight sterilization of being exposed to the sun, with fresh water flushing desilt;
Described water is one-level sand filter seawater, and dissolved oxygen is greater than 3mg/L, salinity 22-33;
Described chlorine-containing disinfectant is commercially available 84 thimerosals;
The husky collection preparation of described diatom and feeding method are: by 1m 2the corrugated plating of/piece tilts 30 ° and towards deep-sea, is invested in seashore beach, after ebb, top layer marine alga is collected together with impurity, be placed in indoor bait and cultivate pond cultivation, indoor bait is cultivated the dark about 0.5-1m in pond, at the bottom of pond, paving 6kg is husky, particle diameter 0.1-1mm, add seawater dark to 1m, discharge Micro Algae auximone 120g, sodium silicate 20g, ooze extract 1L, 10 diatoms that corrugated plating collects are cultivated in every pond, water temperature 28-35 ℃ in pond, successively inflation, while throwing something and feeding, water is drained, leave and take bottom diatom husky, with water, splash to the sand surface of parents culture layer, the collocation seawood meal of throwing something and feeding, constituent is the raw red algae powder of commercially available rain, the weight ratio of sargassum thunbergii powder and spirulina powder is 6:3:1, after mixing particle diameter 1mm sand, be spread on the husky table of parents culture layer,
Described diatom is the beach mixing diatoms that dwells in half floating life and the end and easily adhere to, and main genus kind comprises that twill Trentepohlia, Pinnularia, Navicula, Nitzschia, Chaetoceros belong to and Skeletonema;
The seabed mud that described ooze extract is intertidal zone or trench mud, every 2kg adds water 1L and boils 1h, gets supernatant after precipitation, with 300 mesh sieve thin,tough silk bag filtration one time, leaves and takes filtrate sealing standby.
Take the present invention of above-mentioned measure, by the measure such as lay eggs of Sipunculus nudus parents culture pond structure, the cultivation of indoors artificial bait, accelerating cultivating and managing, control and prevention of disease and Artificial Control, realize artificial indoor parent accelerating and be down to 7% ~ 12% with the lethality of cultivating 40 of laying eggs, parent per unit area yield 5 ~ 70,000 ovum of weight 20g, hatching metamorphosis 13% ~ 86%, and realize and according to production capacity, to carry out Artificial Control and lay eggs.The present invention is the controllability in the solution fertilized egg source of Sipunculus nudus industrial artificial seedling rearing and fine-variety breeding, extend parent egg-laying time, save seedling cost, its material that uses is easy to get, method is handling strong, and the development of propagating industry at Sipunculus nudus artificially has higher practical value.
Embodiment
Embodiment 1
On May 20th, 2010 is carried out artificial indoor culture to the parent of wild population collection, 45 days cultivation time, and concrete implementation step:
(1), indoor culture pond builds: take 2, batch production indoor culture pond, square, floor space 10 m 2, dark 1.2m, intake-discharge pipe is diagonal and distributes, and adopts conduit near discharge outlet, to add water during water inlet.Near discharge outlet 1/4Wei Wusha district, remaining 3/4 is parents culture layer, and centre stacks and separates with building brick level, in order to baffle sand bed, and the thick about 35cm of tile work, brick seals about 1cm.The grit of parents culture layer bottom paving particle diameter 3 ~ 7mm, the about 5cm of thickness, grit layer top covers one deck 50 object screen clothes, and encases every brick, prevents that sand is excessive with water.Sand bed is laid on screen cloth upper strata, thickness 25cm, and the sand of grains of sand footpath 1 ~ 5mm accounts for 30%, and the sand of grains of sand footpath 0.5 ~ 1mm accounts for 60%, and the sand of grains of sand footpath 0.05 ~ 0.5mm accounts for 10%.Sand used, from sea sand, is sieved and is got suitable particle diameter by screen cloth, by using after exposure in sunshine sterilization seawater flushing desilt.Cultivate top, pond 1m and cover sunshade net collapsible, light transmittance 15%, have 5w electricity-saving lamp, in order to control Sipunculus nudus, lay eggs.Every 2.8-3m at the bottom of pond 2standby 1 airstone, keeps dissolved oxygen to be greater than 3mg/L with Roots blower inflation.Single pond is equipped with 10 commercially available heating rods, and pool wall top cover layer of polyethylene film is in order to insulation.
(2), parent selects: wild individuality, Individual Quality >18g, rhynchodaenm tentacle end is without disappearance and body wall without the damaged and concavo-convex trace of pulling, male and female are selected than 5:2, with seawater, repeatedly rinse after choosing 10kg, after be invested in 2 and cultivate on the sand beds of pond.
(3), artitificial food preparation: gather natural diatom from natural waters beach, natural diatom is the beach mixing diatoms that dwells in half floating life and the end and easily adhere to, and main genus kind comprises that twill Trentepohlia, Pinnularia, Navicula, Nitzschia, Chaetoceros belong to and Skeletonema.By 1m 2the corrugated plating of/piece tilts 30 ° to be invested in towards deep-sea the diatoms such as sea area beach and adheres to, rise for 2 times after ebb, corrugated plating top layer marine alga is scraped into collection bag together with impurity, rear according to the demand not timing collection of parents culture and take back indoor bait and cultivate pond and cultivate.Indoor bait is cultivated pond floor space 15m 2the dark 1m in pond spreads about 6kg husky, particle diameter 0.1 ~ 1mm before cultivation at the bottom of every pond, add again one-level sand filter seawater dark to 1m, discharge Micro Algae auximone 120g, sodium silicate 20g, ooze extract 1L, the diatom that 10 corrugated platings are collected is thrown in into indoor bait and cultivates, 28 ~ 32 ℃ of water temperatures, water layer is stirred in continuous charge, cultivates and after 3 days, is divided into 3 ponds cultivations.After diatom culture pond being drained to water before throwing something and feeding, diatom sand at the bottom of pond is scraped and collected, spill and throw in the husky table of parents culture layer.The raw red algae powder of rain, sargassum thunbergii powder and spirulina powder weigh to be mixed thoroughly rear standby in the ratio of 6:3:1 and about 200g sand.
(4), accelerating cultivating and managing: adopt temperature-raising method to cultivate, 25 ℃ of initial water temperatures, slowly heated up 5 ℃ by heating rod in 5 days, were warming up to 30 ℃ and no longer heated up, and it is 32/m that parent density is cultivated in every pond 2, day drains water once, and water is one-level sand filter seawater, salinity 31, the slow water filling in Yu Wusha district again after dry dew 4h, water filling flow velocity is 4m 3/ h, throws something and feeds after draining water, throws in the throw something and feed diatom of 3000g of parent proxima luce (prox. luc) husky, the seawood meal of throwing something and feeding next day, and every pond 5g, throws something and feeds the next day of later in turn.
(5), control and prevention of disease: the parent of selecting is with the Benzylpenicillin sodium salt sea water solution 3L sterilization 15min of 0.1mg/L, cultivate pond and throw something and feed preshoot sand wash layer once, after parent being washed in a pan out in every 10 days with the 84 thimerosals sterilizations parents culture sand beds of available chlorine content 100mg/L, sterilization parents culture layer 30min, with in the sodium thiosulfate sea water solution of 10mg/L and chlorine residue, during water level 0.5m, use sodium thiosulfate 50g, more repeatedly rinse with sand filter seawater, again throw in parent and continue to cultivate.
(6), control and to lay eggs: while promoting production ovum, cultivate water temperature while rising to 30 ℃, with sunshade net shading, the about 1m of the depth of water, drips the ammonia spirit 0.2ml/m of mass fraction 28% 3, after 24h, to lay eggs, restriction is laid eggs and adopt to reduce is cultivated water level to exceeding the about 3cm of sand bed, cancels shading, and night to be to continue electricity-saving lamp illumination, on the rocks being cooled to below 30 °, the ovum that repeats again in the time of need to laying eggs to promote production operation.
(7), cultivate result: use and cultivate 2, pond, parent 10kg, the lethality of cultivating 40 is 11.8%, 5.6 ten thousand ovum of parent per unit area yield of monomer mass 20g, hatching metamorphosis survival rate 13% ~ 68%, the artificial short Sipunculus nudus parent about 24h of used time of laying eggs, limits its egg-laying time and can reach 8, realizes the artificial parents culture of Sipunculus nudus and controls the object of laying eggs.
Embodiment 2
Respectively on June 20th, 2011 and August 14 wild and cultured population being mixed and gather parent, carry out artificial indoor culture, 40 days cultivation time, concrete implementation step:
(1), indoor culture pond builds: adopt indoor cement pit, square, floor space 15 m 2, dark 1m, intake-discharge pipe is diagonal and distributes, and adopts conduit near discharge outlet, to add water during water inlet.Near discharge outlet 1/4, be without sand bed, remaining 3/4 is parents culture layer, and centre stacks and separates with building brick level, in order to baffle sand bed, and the thick about 35cm of tile work, brick seals about 1cm.The grit of parents culture layer bottom paving particle diameter 3 ~ 7mm, the about 5cm of thickness, grit layer top covers one deck 50 object screen clothes, and encases every brick, prevents that sand is excessive with water.Sand bed is laid on screen cloth upper strata, thickness 30cm, and the sand of grains of sand footpath 1 ~ 5mm accounts for 40%, and the sand of grains of sand footpath 0.5 ~ 1mm accounts for 50%, and the sand of grains of sand footpath 0.05 ~ 0.5mm accounts for 10%.Sand used is from river sand and sea sand, mixes and uses, and wherein sea sand rinses twice with fresh water, and the direct exposure in sunshine sterilization of river sand is sieved and got suitable particle diameter and re-use by screen cloth.Cultivate top, pond 1m and cover sunshade net collapsible, light transmittance approximately 10%, have 5w electricity-saving lamp, in order to control Sipunculus nudus, lay eggs.Every about 3m at the bottom of pond 2standby 1 airstone, keeps dissolved oxygen >3mg/L with Roots blower inflation.Single pond is equipped with 12 heating rods, and pool wall top cover layer of polyethylene film is in order to insulation.
(2), parent selects: select wild and cultivation is individual, Individual Quality >18g, rhynchodaenm tentacle end is without disappearance, body wall without damaged and and concavo-convex, without annellation, male and female are selected and are compared 5:2, choose parent and repeatedly rinse with seawater, can not stretch eliminating of tentacle.After be invested in 6 and cultivate on the sand beds of pond.
(3), artitificial food preparation: with embodiment 1.
(4), accelerating cultivating and managing: adopt temperature-raising method to cultivate, 27 ~ 29 ℃ of initial water temperatures, no longer heated up after being slowly warming up to 33 ℃ in 5 days, and it is 30/m that parent density is cultivated in every pond 2.Day drains water once, and water is one-level sand filter seawater, salinity 31, and the slow water filling in Yu Wusha district again after dry dew 4h, water filling flow velocity is 3m 3/ h.After draining water, throw something and feed at every turn, throw in the parent proxima luce (prox. luc) 3000g diatom of throwing something and feeding husky, the seawood meal of throwing something and feeding next day, every pond 5g, throws something and feeds the next day of later in turn.
(5), control and prevention of disease: the parent of selecting is with the Benzylpenicillin sodium salt sea water solution 3L sterilization 5min of 0.5mg/L, the next day of cultivating pond, throw something and feed preshoot sand wash layer once, the 84 thimerosal sterilization parents culture layer 30min that the available chlorine content of take after parent being washed in a pan out in every 7 days is 100mg/L, after with in the sodium thiosulfate sea water solution of 10mg/L and chlorine residue, during water level 0.5m, use the about 50g of sodium thiosulfate, with sand filter seawater, repeatedly rinse again, again throw in parent and continue to cultivate.
(6), control and lay eggs: with embodiment 1.
(7), cultivate result: use and cultivate 6, pond, parent 62kg, the lethality of cultivating 40d is 7 ~ 10%, parent per unit area yield 6.3 ~ 7.0 ten thousand ovum of Individual Quality 20g, hatching metamorphosis survival rate 22% ~ 86%, the artificial short Sipunculus nudus parent about 24 ~ 28h of used time of laying eggs, limits its egg-laying time and can reach 7, realizes the artificial parents culture of Sipunculus nudus and controls the object of laying eggs.

Claims (2)

1. a Sipunculus nudus parents culture method, is characterized in that described method comprises the steps:
(1) indoor culture pond is set: cultivation pond is cement pit, floor space 10-15m 2dark 0.8-1.5m, at the bottom of pond, one end is provided with a discharge outlet, 1/4Wei Wusha district near discharge outlet, remaining 3/4 is parents culture layer, centre stacks and separates with brick, parents culture layer bottom paving particle diameter 3-7mm grit, thickness 4-7cm, covers one deck 30-50 eye mesh screen on grit, and encase every brick, on screen cloth, lay the sand bed of the thick 25-30cm of particle diameter 0.5-5mm, sea water advanced is 35-40cm, cultivates the sunshade net that top, pond 1m arranges retractable light transmittance 10-15%, illuminating lamp is equipped with, about 3m at the bottom of pond in sunshade net top 2place an airstone, every pond is equipped with 10-12 heating rod, and pool wall top cover layer of polyethylene film is in order to insulation;
(2) parent is selected: late May to by the end of August, is selected quality and is greater than 18g, and rhynchodaenm tentacle end is without disappearance and the undamaged individuality of body surface, and female-male proportion is 5:2-3, after choosing with seawater flushing, 28-25 bar/m 2density, be invested on the cultivation layer of cultivating pond;
(3) accelerating cultivating and managing: when cultivating pond maritime interior waters temperature lower than 30 ℃, make temperature rise to 30-33 ℃ of temperature control cultivate with heating rod, original water temperature reaches 30 ℃ to be needed that water temperature is down to 30 ℃ and heat up below again, and programming rate is less than 5 ℃/5 days; Drain water every day once, the dry slow water filling in Yu Wusha district again after 4 hours, the water filling flow velocity 3-4m of revealing 3/ h, the dark 35-40cm of water filling; After draining water, throw something and feed, in throwing in the first 1 day diatom sand of throwing something and feeding of parent, every pond 2800-3200g, throws in the parent seawood meal of throwing something and feeding the same day, and then every pond 5g throws something and feeds the husky and seawood meal of diatom the next day in turn;
(4) control and prevention of disease: while selecting parent with the Benzylpenicillin sodium salt sea water solution of 0.1-0.5mg/L to parent body surface sterilization 10-15min, cultivate before throwing something and feeding in pond and use seawater flushing sand bed once, after every 7-10 day parent being taken out from sand with the chlorine-containing disinfectant sterilization parents culture layer 30min of available chlorine 100mg/L, then with in the sodium thiosulfate sea water solution of 10mg/L and residual chlorine 30min, with sand filter seawater, rinse, more again throw in parent and continue to cultivate;
(5) control and lay eggs: control water temperature at 30-33 ℃, with sunshade net shading, depth of water 0.8-1.2m, the ammonia spirit 0.2ml/m of dropping mass fraction 28% 3, after 24-28h, to lay eggs, restriction is laid eggs to adopt to reduce and is cultivated water level to exceeding the about 3cm of sand bed, cancels shading, and be continuously applied illumination with illuminating lamp night, is cooled to the measure of 1-2 ℃;
Described water is one-level sand filter seawater, and dissolved oxygen is greater than 3mg/L, salinity 22-33;
Described chlorine-containing disinfectant is commercially available 84 thimerosals.
2. a kind of Sipunculus nudus parents culture method as claimed in claim 1, is characterized in that the husky collection preparation of described diatom and feeding method are: by 1m 2the corrugated plating of/piece tilts 30 ° and towards deep-sea, is invested in seashore beach, after ebb, top layer marine alga is collected together with impurity, be placed in indoor bait and cultivate pond cultivation, indoor bait is cultivated the dark about 0.5-1m in pond, at the bottom of pond, paving 6kg is husky, particle diameter 0.1-1mm, add seawater dark to 1m, discharge Micro Algae auximone 120g, sodium silicate 20g, ooze extract 1L, 10 diatoms that corrugated plating collects are cultivated in every pond, water temperature 28-35 ℃ in pond, successively inflation, while throwing something and feeding, water is drained, leave and take bottom diatom husky, with water, splash to the sand surface of parents culture layer, the collocation seawood meal of throwing something and feeding, constituent is the raw red algae powder of commercially available rain, sargassum thunbergii powder and spirulina powder weight ratio are 6:3:1, after mixing particle diameter 1mm sand, be spread on the husky table of parents culture layer,
Described diatom is the beach mixing diatoms that dwells in half floating life and the end and easily adhere to, and main genus kind comprises that twill Trentepohlia, Pinnularia, Navicula, Nitzschia, Chaetoceros belong to and Skeletonema;
The seabed mud that described ooze extract is intertidal zone or trench mud, every 2kg adds water 1L and boils 1h, gets supernatant after precipitation, with 300 mesh sieve thin,tough silk bag filtration one time, leaves and takes filtrate sealing standby.
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CN104304203A (en) * 2014-10-23 2015-01-28 陈振国 Sipunculus nudus trap
CN104770315A (en) * 2015-03-25 2015-07-15 广西壮族自治区海洋研究所 Method for preventing exposure and desiccation of Babylonia aerolata seedlings
CN105724322A (en) * 2016-03-18 2016-07-06 乐山师范学院 Indoor large-scale breeding method for corydalus cornutus larvae and pupae
CN105918172A (en) * 2016-04-29 2016-09-07 海南省海洋与渔业科学院 Ecological culture method for Penaeus vannamei and Sipunculus nudus
CN110915766A (en) * 2019-12-23 2020-03-27 广西壮族自治区海洋研究所 Method for breeding Sipunculus nudus seedlings by using hard bottom pond
CN111066702A (en) * 2019-12-30 2020-04-28 中国水产科学研究院南海水产研究所 Method for indoor high-density breeding of Sipunculus nudus

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* Cited by examiner, † Cited by third party
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CN104304203A (en) * 2014-10-23 2015-01-28 陈振国 Sipunculus nudus trap
CN104770315A (en) * 2015-03-25 2015-07-15 广西壮族自治区海洋研究所 Method for preventing exposure and desiccation of Babylonia aerolata seedlings
CN105724322A (en) * 2016-03-18 2016-07-06 乐山师范学院 Indoor large-scale breeding method for corydalus cornutus larvae and pupae
CN105918172A (en) * 2016-04-29 2016-09-07 海南省海洋与渔业科学院 Ecological culture method for Penaeus vannamei and Sipunculus nudus
CN110915766A (en) * 2019-12-23 2020-03-27 广西壮族自治区海洋研究所 Method for breeding Sipunculus nudus seedlings by using hard bottom pond
CN111066702A (en) * 2019-12-30 2020-04-28 中国水产科学研究院南海水产研究所 Method for indoor high-density breeding of Sipunculus nudus

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