CN110915766A - Method for breeding Sipunculus nudus seedlings by using hard bottom pond - Google Patents
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- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
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Abstract
The invention relates to the technical field of Sipunculus nudus breeding, and discloses a method for breeding Sipunculus nudus seedlings by using a hard bottom pond. The method has the advantages of scientific and reasonable technological operation flow, simple and understandable operation method, simple and direct operation, reduction of labor intensity of workers, improvement of survival rate of seedlings during seedling transition, cost saving and income improvement, great promotion effect on the industrial development of artificial seedlings of the sipunculus nudus, creativity and higher practical value, and great significance on protecting and developing ocean resources, flourishing economy, enriching the variety structure of mariculture and implementing sustainable development strategy.
Description
Technical Field
The invention relates to the technical field of Sipunculus nudus breeding, in particular to a method for breeding Sipunculus nudus seedlings by using a hard bottom pond.
Background
Sipunculusnudus (Sipunculusnudus) is also called Sipunculus nudus, commonly called sandworm, belonging to Sipunculus animal phylum (Sipuncula), Sipunculus, and is shaped like a sausage, and has a long cylindrical shape, a body length of about 10-20 cm, bare hair-free and latticed patterns on the body wall. The Sipunculus nudus is a global distribution type and is distributed in the south coast of Qingdao in China, wherein Fujian and Liangguang coast have abundant resources, so that the Sipunculus nudus is one of famous seafood, and is deeply popular with domestic and foreign markets due to delicious taste and rich nutrition. The variety takes organic debris on the mudflat and benthic algae as food, the food chain level is low, the stress resistance is strong, and the breeding ecology is very suitable for large-area artificial breeding. At present, along with the industrialized development of Sipunculus nudus breeding, the indoor benthic offspring seed breeding operation is complicated, the workload of workers is large after the expanded production, and meanwhile, when the indoor offspring seed is transferred to a pond for standard rough breeding, the survival rate of the offspring seed can be influenced by overhigh or overlow water pressure.
Therefore, the inventor provides a seedling breeding method for the Sipunculus nudus in the hard bottom pond according to the physiology and life habits of the Sipunculus nudus and by combining years of indoor seedling breeding and pond intermediate breeding tests of the inventor, the method can stabilize the metamorphosis rate, and has great significance for reducing the workload of workers, improving the survival rate of the seedlings in the process of transferring to the intermediate breeding field and improving the economic benefit.
Disclosure of Invention
Based on the problems, the invention provides a method for breeding Sipunculus nudus seedlings by using a hard bottom pond, provides a new way for the large-scale breeding and culture of the Sipunculus nudus indoor seedlings, has scientific management means, is reasonable and simple to operate, simplifies the operation procedures, reduces the labor intensity of workers, improves the survival rate of the seedlings during seedling transition, and has the practical advantages of saving cost and improving income.
In order to solve the technical problems, the invention provides a method for breeding Sipunculus nudus seedlings by using a hard bottom pond, which comprises the following steps:
s1: pond with hard bottom
Selecting a hard bottom pond with a cement surface at the bottom, wherein the inclination of the bottom of the hard bottom pond is 5 +/-3%, the hard bottom pond is positioned in a zone which is free of oil stains and far away from domestic sewage, the hard bottom pond is provided with a water gate capable of naturally receiving water during heavy tide, the water gate is nailed with a 120-mesh nylon net, the water gate is provided with two water inlets with the diameter of 250mm, a water pump is installed at each water inlet, a water outlet of the water pump is provided with a 120-mesh nylon bag with the length of 5m, and the nylon bag is positioned in the hard bottom pond;
s2: pretreatment of hard bottom ponds
Bottom sand with the thickness of 8-10 cm is paved at the bottom of the hard bottom pond, 100 kilograms of quicklime is spread in the hard bottom pond before water is received, and when the water receiving amount is not less than 100cm, the hard bottom pond is subjected to fertilizer water treatment 2 days before seedling placement;
s3: selection and temporary rearing of parents
Selecting Sipunculus nudus with weight not less than 15g as parent, placing the parent in room for temporary rearing and fattening, and naturally producing fertilized eggs during the temporary rearing and fattening process;
s4: management of planktonic larvae
Collecting the fertilized eggs in the step S3 by using a 250-mesh bolting silk bag, and transferring the fertilized eggs to an indoor planktonic larva cultivation pond for cultivation;
s5: management of metamorphosis period
2 days before the metamorphosis period, feeding 60-80cm of water into the hard bottom pond, sprinkling benthic diatom liquid serving as a target algae species, sprinkling amino acid, water and fertilizer paste and water, then putting the planktonic larvae cultured for 6-9 days in the step S4 into the hard bottom pond, and continuously oxygenating during the metamorphosis period; after the metamorphosis period, performing water changing treatment on the surface layer of the hard bottom pond, continuously changing water for 1h every day, and continuing the water changing treatment for 5 days;
s6: juvenile sipunculus management
After the surface layer of the hard bottom pond is changed with water for 5 days, the whole pond is changed with water, the surface of the sand layer is cleaned firstly when the water is changed, then the water is fed again, 2kg of bottom-changing probiotic bacillus subtilis is sprinkled on the hard bottom pond, and the water level of the hard bottom pond is maintained at 80 cm; applying amino acid fertilizer water paste to the hard bottom pond, wherein the application amount of the amino acid fertilizer water paste is that the visibility of the water body in the hard bottom pond is 60cm, the continuous oxygenation state is kept in the process, and the operation is repeated every half month;
s7: harvesting
Cultivating until the quantity of the sipunculus nudus reaches 1600 pieces/kg, digging seedlings and harvesting the seedlings.
Further, the bottom sand in the step S2 includes the following components in percentage by mass: the sand comprises fine sand with the mass fraction not less than 30%, medium sand with the mass fraction not less than 30% and coarse sand with the mass fraction not more than 30%, wherein the content of mud is not more than 20%.
Furthermore, the fine sand is sand with an average grain diameter of 0.125-0.25 mm, the medium sand is sand with an average grain diameter of 0.25-0.5 mm, and the coarse sand is sand with an average grain diameter of 0.5-1 mm.
Further, the bottom sand in the step S2 includes the following components in percentage by mass: 35% of fine sand, 45% of medium sand and 20% of coarse sand.
Further, the parents selected in step S3 have the following signs: parent body surface has no scar, body color is dry and rapidly turns light red in the open air, body is tightly pressed and tense, the osculum is flexible and powerful, gonad is healthy, the particle size of egg cells is large, and sperm cells are flocculent and agglomerated.
Further, the management conditions of the planktonic larvae culture pond in step S4 are as follows: the seawater in the planktonic larva culture pond is secondary sand-filtered seawater, and the salinity of the seawater is 25-33 per mill; the bait in the floating larva cultivating pool is mainly unicellular algae, and the feeding amount is that the floating larva still presents a full stomach shape after 8 hours of the unicellular algae bait is added; the planktonic larva cultivation pool adopts a full shading mode, and the light transmittance is less than or equal to 10 percent; continuously oxygenating during the cultivation period, and maintaining the dissolved oxygen amount in the planktonic larva cultivation pool at 7.7 +/-0.5 mg/L; the water temperature of the planktonic larvae in the culture pond is maintained at 26-32 ℃ throughout the day.
Compared with the prior art, the invention has the beneficial effects that: the method has the advantages of scientific and reasonable process operation flow, simple and understandable operation method, simple and direct operation, reduced labor intensity of workers, improved survival rate of seedlings when the seedlings are transferred, cost saving and income improvement, fills in the blank of seedling breeding of the Sipunculus nudus hard bottom pond, and realizes high-density seedling breeding of the Sipunculus nudus hard bottom pond; according to the invention, by managing planktonic larvae and juvenile sipunculus by stages, the influence of accumulation of residual baits and feces at the bottom of the pond on the metamorphosis survival rate of larvae is reduced; by carrying out physical micro-treatment on the sand surface of the hard bottom pond and utilizing the life habit of the Sipunculus nudus, the Sipunculus nudus quickly adapts to the substrate environment of the hard bottom pond in the metamorphosis period, the metamorphosis rate of larvae is stabilized, the metamorphosis survival rate is over 80 percent, and the yield per mu can reach 68.32 ten thousand; compared with the breeding of the seedlings in the soil pond, the hard-bottom pond is beneficial to physical and biological regulation and control of the substrate environment, and the digging is convenient; compared with the traditional indoor seedling breeding of the sipunculus nudus, the metamorphosis rate is not lower than that of indoor workshop larvae, and meanwhile, the loss of the sipunculus nudus from a workshop to a midculture pond is reduced. The invention has great promotion effect on the industrialized development of the artificial seedling of the Sipunculus nudus, creativity and higher practical value, and has important significance for protecting and developing ocean resources, flourishing economy, enriching the structure of mariculture varieties and implementing sustainable development strategy.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail below with reference to examples, and the exemplary embodiments and descriptions thereof are only used for explaining the present invention and are not used as limitations of the present invention.
Example 1:
a method for breeding Sipunculus nudus seedlings by using a hard bottom pond comprises the following steps:
s1: selection of hard bottom ponds
Selecting a hard bottom pond with a cement surface at the bottom, wherein the pond area is 3.8 mu, the inclination of the bottom of the hard bottom pond is 5 +/-3%, and the hard bottom pond is located in a zone without oil stains and far away from domestic sewage, and all toxic insect drugs are prohibited to be put in the zone; the hard bottom pond is provided with a water gate capable of naturally receiving water during heavy tide, the water gate is nailed with a 120-mesh nylon net, the water gate is provided with two water inlets with the diameter of 250mm, a 2000 kilowatt submersible pump is mounted at the water inlets, a 5m nylon bag with the length of 120 meshes is sleeved at a water outlet of the submersible pump, and the nylon bag is positioned in the hard bottom pond;
s2: hard bottom pond pretreatment
Bottom sand with the thickness of 8-10 cm is paved at the bottom of the hard bottom pond, and the bottom sand comprises the following components in percentage by mass: spreading 100 kilograms of quicklime into the whole pond of the hard bottom pond before receiving water by 35 percent of fine sand, 45 percent of medium sand and 20 percent of coarse sand, wherein when the water receiving amount is not less than 100cm, the water receiving amount is 120cm in the embodiment, performing water fertilizing treatment on the hard bottom pond 2 days before putting seedlings, and inoculating unicellular algae seeds such as diatom, flat algae and the like and spraying amino acid water fertilizing paste to perform early-stage algae culture in the embodiment;
s3: seedling cultivation method
Purchasing 20 kilograms of wild healthy sipunculus nudus parents, directly putting the wild healthy sipunculus nudus parents into a cultivation pond with the visibility of 60cm, and breeding seedlings in a hard-bottom pond in a natural spawning mode;
s4: planktonic phase management
The salinity of the seawater in the step is 25-33 per thousand, a water sample is taken every day, the spawning condition and the death condition of the parents are checked, the ingestion and growth condition of the planktonic larvae are microscopically checked, the fact that the cultivation period of the planktonic larvae lasts for 16 days all the time is found, the spawning period is from the third day to the seventh day after the parents are put in, the daily average spawning amount is 73.5 ten thousand, and the spawning amount of the parents in the batch is accumulated to 367.5 thousand; supplementing amino acid fertilizer water paste every day, maintaining visibility of 60cm, keeping continuous oxygenation in the pond, and maintaining dissolved oxygen at 7.7 +/-0.5 mg/L;
s5: management of metamorphosis stage
Observing planktonic larvae through microscopic examination, weighing 200g of prawn slices per day in the early stage of metamorphosis, fermenting for 24h, sprinkling into a cultivation pond, and sprinkling diatom liquid cultivated in an indoor workshop, so as to ensure that the visibility reaches 50 cm; stopping splashing the fermented shrimp slices after no planktonic larvae are found in the water body, installing a PVC short pipe with the height of 80cm at a water outlet, pumping water for 1h by using a 2000W submersible pump every day, discharging surface water from the upper part of the PVC short pipe, and continuing the operation for 5 days;
s6: management of juvenile sipunculus
After the surface layer of the hard bottom pond is changed with water for 5 days, the whole pond is changed with water, when the water is changed, a small-power water pump gateway is connected with a sprinkler to lightly clean the surface of the sand layer so as to clean the excessive benthic diatoms on the surface of the sand layer, then water is re-fed, and the water level of the hard bottom pond is maintained at 80 cm; after the treatment is finished, applying amino acid fertilizer water paste and fertilizer water to the hard bottom pond, wherein the application amount of the amino acid fertilizer water paste is preferably that the visibility of the water body in the hard bottom pond is 60cm, the oxygen charging state is kept continuously in the process, and the operation is repeated every half month.
S7: harvesting
In the embodiment, after 75 days of breeding, the number of seedlings is counted by sampling, the seedlings are manually picked, 7 square sample blocks of 100cm × 100cm are sampled, 846 sample blocks, 757 sample blocks, 795 sample blocks, 573 sample blocks, 854 sample blocks, 1061 sample blocks and 736 sample blocks are respectively sampled from seven sampling sample blocks, average 803.14 sample blocks/m 2 sample the output of the hard bottom pond is calculated to be about 203.56 ten thousand, the mass of a sample body is 0.38-0.64 g, and Sipunculus nudus individuals with the physical mass of more than or equal to 0.5g account for 38.7 percent. After 75 days of cultivation, the metamorphosis survival rate of the larvae is 55.39 percent.
Example 2:
a method for breeding Sipunculus nudus seedlings by using a hard bottom pond comprises the following steps:
s1: pond with hard bottom
Selecting a hard bottom pond with a cement surface at the bottom, wherein the pond area is 3.8 mu, the inclination of the bottom of the hard bottom pond is 5 +/-3%, and the hard bottom pond is located in a zone without oil stains and far away from domestic sewage, and all toxic insect drugs are prohibited to be put in the zone; the hard bottom pond is provided with a water gate capable of naturally receiving water during heavy tide, the water gate is nailed with a 120-mesh nylon net, the water gate is provided with two water inlets with the diameter of 250mm, a 2000 kilowatt submersible pump is mounted at the water inlets, a 5m nylon bag with the length of 120 meshes is sleeved at a water outlet of the submersible pump, and the nylon bag is positioned in the hard bottom pond;
s2: pretreatment of hard bottom ponds
Bottom sand with the thickness of 8-10 cm is paved at the bottom of the hard bottom pond, and the bottom sand in the embodiment comprises the following components in percentage by mass: the sand comprises fine sand with the mass fraction not less than 30%, medium sand with the mass fraction not less than 30%, coarse sand with the mass fraction not more than 30%, wherein the content of mud is not more than 20%, the fine sand in the embodiment is sandstone with the average particle size of 0.125-0.25 mm, the medium sand is sandstone with the average particle size of 0.25-0.5 mm, and the coarse sand is sandstone with the average particle size of 0.5-1 mm, the purpose of selecting bottom sand with different particle sizes in the embodiment is to meet the requirements of different specifications on different sand particle sizes, and increase gaps among sand particles, so that the permeation effect of bottom materials in a drainage period is increased, the permeation of dissolved oxygen and organic matters is facilitated, and a guarantee is provided for improving the survival rate and the growth speed of Sipunculus nudus Linnaeus; before water collection, 100 kilograms of quicklime is spread in the whole pond of the hard bottom pond, when the water collection amount is not less than 100cm, the water collection amount is 120cm, the hard bottom pond is subjected to water fertilizing treatment 2 days before seedling placement, and the early-stage algae cultivation is carried out by splashing and fermenting chicken manure and amino acid water fertilizing paste;
s3: selection and temporary rearing of parents
Purchasing 20 kg of wild healthy Sipunculus nudus parents, selecting the Sipunculus nudus with the weight not less than 15g as the parents, wherein the selected parents have the following physical signs: parent body surface has no scar, body color is dry and rapidly turns light red in the open air, body is tightly pressed and tense, the osculum is flexible and powerful, gonad is healthy, the particle size of egg cells is large, and sperm cells are flocculent and agglomerated; putting the parents into a room for temporary rearing and fattening, and naturally producing the parents in the temporary rearing and fattening process to obtain fertilized eggs;
s4: management of planktonic larvae
Collecting the fertilized eggs in the step S3 by using a 250-mesh bolting silk bag, transferring the fertilized eggs into an indoor planktonic larva cultivation pool for cultivation, wherein the seawater in the planktonic larva cultivation pool is secondary sand-filtered seawater, and the salinity of the seawater is 25-33 per mill; the bait in the floating larva cultivating pool is mainly unicellular algae, the chaetoceros and the platymonas are fed in the embodiment, and the feeding amount is that the floating larva still presents a full stomach shape after the unicellular algae bait is fed for 8 hours; the planktonic larva cultivation pool adopts a full shading mode, and the light transmittance is less than or equal to 10 percent; continuously oxygenating during the cultivation period, and maintaining the dissolved oxygen amount in the planktonic larva cultivation pool at 7.7 +/-0.5 mg/L; the water temperature of the planktonic larvae in the culture pond is maintained at 26-30 ℃ throughout the day; the step of the embodiment is used for indoor cultivation of the planktonic larvae, so that not only can the larvae be fully supplied with nutrition, but also the growth of the larvae is not influenced by climatic factors, and the prevention and the control on diseases are realized;
s5: management of metamorphosis period
Inoculating diatom and tetraselmis algae liquid and splashing amino acid and fertilizer water paste into a hard bottom pond 2 days before the larva is placed in the pond, splashing 3kg of fertilizer water paste for the first time, wherein the visibility of the hard bottom pond is 50cm after 2 days, then putting the planktonic larva cultured for 9 days in the step S4 into the hard bottom pond, continuously oxygenating during the metamorphosis period, and collecting 320 million planktonic larvae after the planktonic larva is cultured in the planktonic larva culturing pond for 9 days; after the seedlings are placed in the pond, 200g of the prawn slices are weighed every day, fermented for 24 hours and then sprinkled into the cultivation pond, after 3 days of floating larvae, almost all the prawn slices are settled and metamorphosed, and at the moment, sprinkling the fermented prawn slices is stopped. After the metamorphosis period, a PVC short pipe with the height of 80cm is installed at a water outlet of the hard bottom pond, water is pumped for 3 hours by a 2000W water pump every day, surface water is discharged from the upper part of the PVC short pipe, water is continuously changed for 1 hour every day, and water changing treatment lasts for 5 days; in the embodiment, the planktonic larvae bred in the same time are put into the hard bottom pond, the metamorphosis in the same period can be achieved in the shortest time, the influence of weather factors or other non-antibiotic factors on the growth of the planktonic larvae in the water body is reduced, and after the larvae are metamorphosis, the influence caused by the transient lack of benthic diatoms can be achieved by feeding substitutes such as organic debris;
s6: juvenile sipunculus management
After the surface layer of the hard bottom pond is changed with water for 5 days, the whole pond is changed with water, when the water is changed, a low-power water pump gateway is firstly connected with a shower head to lightly clean the surface of the sand layer so as to clean the excessive benthic diatoms on the surface of the sand layer, then water is re-fed, then bottom-changing probiotics are sprinkled on the hard bottom pond, and the water level of the hard bottom pond is maintained at 80 cm; according to the method, the surface of the sand layer is lightly washed and microbial bottom-changing treatment is carried out according to the physiological and living characteristics of the Sipunculus nudus, so that the accumulation of excess benthic diatoms and other baits and organic matters which cannot be utilized is further reduced in the cultivation process, the distribution of surface sand is improved, the permeation of the substrate and the number of the grain sizes of the sand which can be grabbed by the lip of the Sipunculus nudus are further ensured, and the growth of the Sipunculus nudus is facilitated; after the treatment is finished, applying amino acid fertilizer water paste and fertilizer water to the hard bottom pond, wherein the application amount of the amino acid fertilizer water paste is preferably that the visibility of the water body in the hard bottom pond is 60cm, the oxygen charging state is kept continuously in the process, and the operation is repeated every half month;
s7: harvesting
Cultivating until the quantity of the sipunculus nudus reaches 1600 pieces/kg, digging seedlings and harvesting the seedlings; in the embodiment, the seedlings are harvested after 75 days of breeding, the number of the seedlings is counted by sampling, the seedlings are manually harvested, 7 square sample blocks of 100cm multiplied by 100cm are sampled, seven sample blocks are respectively sampled by 1056, 1008, 1035, 1016, 1072, 981 and 1003, and average 1024.43/m2Calculating the output of the hard bottom pond to be about 259.65 thousands of samples, wherein the mass of the sample body is 0.41-0.60 g, and statistics shows that the Sipunculus nudus with the physical mass of not less than 0.5g accounts for 43.5 percent. After 75 days of cultivation, the metamorphosis survival rate of the larva reaches 81.14%.
Example 3:
the procedure of this example 3 is the same as that of example 2, except that the bottom sand in step S3 includes the following components in mass fraction: 35% of fine sand, 45% of medium sand and 20% of coarse sand. And (4) putting the planktonic larvae cultured for 6 days in the step S4 into a hard bottom pond, wherein the temperature for culturing the planktonic larvae is 28-33 ℃.
Cultivating until the quantity of the sipunculus nudus reaches 1600 pieces/kg, digging seedlings and harvesting the seedlings; in the embodiment, after 75 days of breeding, the seedlings are harvested, the number of the seedlings is counted by sampling, the seedlings are manually collected, 7 square sample blocks of 100cm multiplied by 100cm are sampled, and 1015, 1046, 925, 1087, 1034, 1007 and 956 samples are respectively sampled from seven sampling sample blocks, and the average is 1024.43/m2The yield of the pond with the hard bottom is calculated to be about 255.99 ten thousand, the mass of a sample body is 0.38-0.68 g, and statistics shows that Sipunculus nudus individuals with the physical mass of not less than 0.5g account for 48.7%. After 75 days of cultivation, the metamorphosis survival rate of the larva reaches 80.0 percent.
The above is an embodiment of the present invention. The embodiments and specific parameters in the embodiments are only for the purpose of clearly illustrating the process of verifying the invention and are not intended to limit the scope of the invention, which is defined by the claims, and all the equivalent structural changes made by applying the content of the specification of the invention should be covered by the scope of the invention.
Claims (6)
1. A method for breeding Sipunculus nudus seedlings by using a hard bottom pond is characterized by comprising the following steps:
s1: pond with hard bottom
Selecting a hard bottom pond with a cement surface at the bottom, wherein the inclination of the bottom of the hard bottom pond is 5 +/-3%, the hard bottom pond is positioned in a zone which is free of oil stains and far away from domestic sewage, the hard bottom pond is provided with a water gate capable of naturally receiving water during heavy tide, the water gate is nailed with a 120-mesh nylon net, the water gate is provided with two water inlets with the diameter of 250mm, a water pump is installed at each water inlet, a water outlet of the water pump is provided with a 120-mesh nylon bag with the length of 5m, and the nylon bag is positioned in the hard bottom pond;
s2: pretreatment of hard bottom ponds
Bottom sand with the thickness of 8-10 cm is paved at the bottom of the hard bottom pond, 100 kilograms of quicklime is spread in the hard bottom pond before water is received, and when the water receiving amount is not less than 100cm, the hard bottom pond is subjected to fertilizer water treatment 2 days before seedling placement;
s3: selection and temporary rearing of parents
Selecting Sipunculus nudus with weight not less than 15g as parent, placing the parent in room for temporary rearing and fattening, and naturally producing fertilized eggs during the temporary rearing and fattening process;
s4: management of planktonic larvae
Collecting the fertilized eggs in the step S3 by using a 250-mesh bolting silk bag, and transferring the fertilized eggs to an indoor planktonic larva cultivation pond for cultivation;
s5: management of metamorphosis period
2 days before the metamorphosis period, feeding 60-80cm of water into the hard bottom pond, sprinkling benthic diatom liquid serving as a target algae species, sprinkling amino acid, water and fertilizer paste and water, then putting the planktonic larvae cultured for 6-9 days in the step S4 into the hard bottom pond, and continuously oxygenating during the metamorphosis period; after the metamorphosis period, performing water changing treatment on the surface layer of the hard bottom pond, continuously changing water for 1h every day, and continuing the water changing treatment for 5 days;
s6: juvenile sipunculus management
After the surface layer of the hard bottom pond is changed with water for 5 days, the whole pond is changed with water, the surface of the sand layer is cleaned firstly when the water is changed, then the water is fed again, 2kg of bottom-changing probiotic bacillus subtilis is sprinkled on the hard bottom pond, and the water level of the hard bottom pond is maintained at 80 cm; applying amino acid fertilizer water paste to the hard bottom pond, wherein the application amount of the amino acid fertilizer water paste is that the visibility of the water body in the hard bottom pond is 60cm, the continuous oxygenation state is kept in the process, and the operation is repeated every half month;
s7: harvesting
Cultivating until the quantity of the sipunculus nudus reaches 1600 pieces/kg, digging seedlings and harvesting the seedlings.
2. The method for breeding Sipunculus nudus seedlings in the hard bottom pond as claimed in claim 1, wherein the bottom sand in step S2 comprises the following components by weight: the sand comprises fine sand with the mass fraction not less than 30%, medium sand with the mass fraction not less than 30% and coarse sand with the mass fraction not more than 30%, wherein the content of mud is not more than 20%.
3. The method for breeding Sipunculus nudus seedlings by using the hard bottom pond as claimed in claim 2, wherein the fine sand is sand with an average particle size of 0.125-0.25 mm, the medium sand is sand with an average particle size of 0.25-0.5 mm, and the coarse sand is sand with an average particle size of 0.5-1 mm.
4. The method for breeding Sipunculus nudus seedlings in the hard bottom pond as claimed in claim 2, wherein the bottom sand in step S2 comprises the following components by weight: 35% of fine sand, 45% of medium sand and 20% of coarse sand.
5. The method for the breeding of Sipunculus nudus seedlings in a hard bottom pond as claimed in claim 1, wherein the parents selected in step S3 have the following signs: parent body surface has no scar, body color is dry and rapidly turns light red in the open air, body is tightly pressed and tense, the osculum is flexible and powerful, gonad is healthy, the particle size of egg cells is large, and sperm cells are flocculent and agglomerated.
6. The method for the breeding of the Sipunculus nudus seedlings in the hard bottom pond as claimed in claim 1, wherein the management conditions of the planktonic larvae rearing pond in the step S4 are as follows: the seawater in the planktonic larva culture pond is secondary sand-filtered seawater, and the salinity of the seawater is 25-33 per mill; the bait in the floating larva cultivating pool is mainly unicellular algae, and the feeding amount is that the floating larva still presents a full stomach shape after 8 hours of the unicellular algae bait is added; the planktonic larva cultivation pool adopts a full shading mode, and the light transmittance is less than or equal to 10 percent; continuously oxygenating during the cultivation period, and maintaining the dissolved oxygen amount in the planktonic larva cultivation pool at 7.7 +/-0.5 mg/L; the water temperature of the planktonic larvae in the culture pond is maintained at 26-32 ℃ throughout the day.
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