CN103733063B - 偶联方法 - Google Patents
偶联方法 Download PDFInfo
- Publication number
- CN103733063B CN103733063B CN201280037545.9A CN201280037545A CN103733063B CN 103733063 B CN103733063 B CN 103733063B CN 201280037545 A CN201280037545 A CN 201280037545A CN 103733063 B CN103733063 B CN 103733063B
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- Prior art keywords
- pore
- analyte
- membrane
- polynucleotide
- dna
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y15/00—Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
- G01N27/44743—Introducing samples
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/483—Physical analysis of biological material
- G01N33/487—Physical analysis of biological material of liquid biological material
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/483—Physical analysis of biological material
- G01N33/487—Physical analysis of biological material of liquid biological material
- G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
- G01N33/48721—Investigating individual macromolecules, e.g. by translocation through nanopores
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Nanotechnology (AREA)
- Biophysics (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Crystallography & Structural Chemistry (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Electrochemistry (AREA)
- Cell Biology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201161490860P | 2011-05-27 | 2011-05-27 | |
| US61/490,860 | 2011-05-27 | ||
| US201261599246P | 2012-02-15 | 2012-02-15 | |
| US61/599,246 | 2012-02-15 | ||
| PCT/GB2012/051191 WO2012164270A1 (en) | 2011-05-27 | 2012-05-25 | Coupling method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103733063A CN103733063A (zh) | 2014-04-16 |
| CN103733063B true CN103733063B (zh) | 2016-04-20 |
Family
ID=46210300
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201280037545.9A Active CN103733063B (zh) | 2011-05-27 | 2012-05-25 | 偶联方法 |
Country Status (11)
| Country | Link |
|---|---|
| US (9) | US10246741B2 (enExample) |
| EP (5) | EP4273092B1 (enExample) |
| JP (2) | JP6480183B2 (enExample) |
| KR (1) | KR101963918B1 (enExample) |
| CN (1) | CN103733063B (enExample) |
| AU (1) | AU2012264497B2 (enExample) |
| BR (1) | BR112013030529B1 (enExample) |
| CA (1) | CA2837306C (enExample) |
| IL (1) | IL229540B (enExample) |
| SG (1) | SG10201604316WA (enExample) |
| WO (1) | WO2012164270A1 (enExample) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2025000432A1 (zh) | 2023-06-30 | 2025-01-02 | 深圳华大生命科学研究院 | 利用封闭子进行纳米孔测序的方法 |
Families Citing this family (135)
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|---|---|---|---|---|
| EP2391732B1 (en) | 2009-01-30 | 2015-05-27 | Oxford Nanopore Technologies Limited | Methods using adaptors for nucleic acid constructs in transmembrane sequencing |
| GB0905140D0 (en) | 2009-03-25 | 2009-05-06 | Isis Innovation | Method |
| BR112013020411B1 (pt) | 2011-02-11 | 2021-09-08 | Oxford Nanopore Technologies Limited | Monômero de msp mutante, construto, polinucleotídeo, poro, kit e aparelho para caracterizar uma sequência de ácido nucleico alvo, e, método para caracterizar uma sequência de ácido nucleico alvo |
| SG10201604316WA (en) | 2011-05-27 | 2016-07-28 | Oxford Nanopore Tech Ltd | Coupling method |
| KR20140050067A (ko) | 2011-07-25 | 2014-04-28 | 옥스포드 나노포어 테크놀로지즈 리미티드 | 막횡단 포어를 사용한 이중 가닥 폴리뉴클레오티드 서열분석을 위한 헤어핀 루프 방법 |
| EP3663412B1 (en) | 2011-09-23 | 2022-03-09 | Oxford Nanopore Technologies PLC | Analysis of a polymer comprising polymer units by means of translocation through a nanopore |
| CN104039979B (zh) | 2011-10-21 | 2016-08-24 | 牛津纳米孔技术公司 | 使用孔和Hel308解旋酶表征目标多核苷酸的酶方法 |
| EP2798083B1 (en) | 2011-12-29 | 2017-08-09 | Oxford Nanopore Technologies Limited | Method for characterising a polynucelotide by using a xpd helicase |
| CA2861808C (en) | 2011-12-29 | 2021-02-23 | Oxford Nanopore Technologies Limited | Enzyme method |
| CA2864035C (en) | 2012-02-15 | 2021-05-18 | Oxford Nanopore Technologies Limited | A method for determining the presence of an analyte using an aptamer |
| EP3736339B1 (en) | 2012-02-16 | 2022-07-27 | Oxford Nanopore Technologies plc | Analysis of measurements of a polymer |
| WO2013153359A1 (en) | 2012-04-10 | 2013-10-17 | Oxford Nanopore Technologies Limited | Mutant lysenin pores |
| US11155860B2 (en) | 2012-07-19 | 2021-10-26 | Oxford Nanopore Technologies Ltd. | SSB method |
| CA2879355C (en) | 2012-07-19 | 2021-09-21 | Oxford Nanopore Technologies Limited | Helicase construct and its use in characterising polynucleotides |
| AU2013291763B2 (en) | 2012-07-19 | 2019-06-20 | Oxford Nanopore Technologies Limited | Modified helicases |
| WO2014041337A1 (en) | 2012-09-14 | 2014-03-20 | Oxford Nanopore Technologies Limited | Sample preparation method |
| WO2014072703A1 (en) | 2012-11-06 | 2014-05-15 | Oxford Nanopore Technologies Limited | Quadruplex method |
| WO2014074922A1 (en) | 2012-11-09 | 2014-05-15 | Stratos Genomics, Inc. | Concentrating a target molecule for sensing by a nanopore |
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| GB201313477D0 (en) | 2013-07-29 | 2013-09-11 | Univ Leuven Kath | Nanopore biosensors for detection of proteins and nucleic acids |
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| GB201316849D0 (en) * | 2013-09-23 | 2013-11-06 | Isis Innovation | Method |
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| JP6378924B2 (ja) * | 2014-04-23 | 2018-08-22 | 株式会社日立ハイテクノロジーズ | 核酸分析装置および核酸分析方法 |
| GB201417712D0 (en) | 2014-10-07 | 2014-11-19 | Oxford Nanopore Tech Ltd | Method |
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| CN119899249A (zh) | 2014-09-01 | 2025-04-29 | 弗拉芒区生物技术研究所 | 突变csgg孔 |
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