CN103710427B - 一种鸡基因的单核苷酸多态性、检测方法及其应用 - Google Patents
一种鸡基因的单核苷酸多态性、检测方法及其应用 Download PDFInfo
- Publication number
- CN103710427B CN103710427B CN201310450040.1A CN201310450040A CN103710427B CN 103710427 B CN103710427 B CN 103710427B CN 201310450040 A CN201310450040 A CN 201310450040A CN 103710427 B CN103710427 B CN 103710427B
- Authority
- CN
- China
- Prior art keywords
- chicken
- gene
- mirna
- site
- genotype
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/124—Animal traits, i.e. production traits, including athletic performance or the like
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/156—Polymorphic or mutational markers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/178—Oligonucleotides characterized by their use miRNA, siRNA or ncRNA
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Description
体系成分 | 体积(μL) |
2Taq PCR Master Mix(MBI) | 12 |
正向引物(10pmol/L) | 1 |
反向引物(10pmol/L) | 1 |
DNA模板(100ng/μL) | 0.5 |
灭菌超纯水(ddH2O) | 10.5 |
总体积 | 25.0 |
Claims (4)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201310450040.1A CN103710427B (zh) | 2013-09-27 | 2013-09-27 | 一种鸡基因的单核苷酸多态性、检测方法及其应用 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201310450040.1A CN103710427B (zh) | 2013-09-27 | 2013-09-27 | 一种鸡基因的单核苷酸多态性、检测方法及其应用 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN103710427A CN103710427A (zh) | 2014-04-09 |
CN103710427B true CN103710427B (zh) | 2015-06-17 |
Family
ID=50403781
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201310450040.1A Expired - Fee Related CN103710427B (zh) | 2013-09-27 | 2013-09-27 | 一种鸡基因的单核苷酸多态性、检测方法及其应用 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN103710427B (zh) |
Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN110317885A (zh) * | 2019-08-05 | 2019-10-11 | 红河学院 | 一种检测大围山微型鸡bpi基因snp的pcr-rflp方法 |
CN110592238B (zh) * | 2019-10-16 | 2022-04-15 | 西北农林科技大学 | 与乌鸡生长性状相关的snp标记及其应用 |
CN110872612B (zh) * | 2019-12-19 | 2021-02-09 | 南昌师范学院 | Vipr2基因3’调控区位点与鸡种睾丸性状相关性的检测方法及应用 |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102220314A (zh) * | 2010-04-13 | 2011-10-19 | 中国农业大学 | 辅助鉴定具有不同体重性状的鸡群体的方法 |
CN102250889A (zh) * | 2011-07-14 | 2011-11-23 | 四川农业大学 | 鸡屠体性状相关的snp及其应用 |
-
2013
- 2013-09-27 CN CN201310450040.1A patent/CN103710427B/zh not_active Expired - Fee Related
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102220314A (zh) * | 2010-04-13 | 2011-10-19 | 中国农业大学 | 辅助鉴定具有不同体重性状的鸡群体的方法 |
CN102250889A (zh) * | 2011-07-14 | 2011-11-23 | 四川农业大学 | 鸡屠体性状相关的snp及其应用 |
Non-Patent Citations (1)
Title |
---|
鸡基因组pre-microRNA SNP多态性;耿立英等;《生物多样性》;20091231;第17卷(第3期);第248-256页 * |
Also Published As
Publication number | Publication date |
---|---|
CN103710427A (zh) | 2014-04-09 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN108220408B (zh) | 一种节粮型青胫隐性白羽肉鸡新品系的选育方法 | |
CN108676897B (zh) | 一种影响猪日增重性状的snp标记及其应用 | |
CN104962634B (zh) | 用于检测鸡体尺性状的试剂盒及鸡的分子育种方法 | |
CN104673902B (zh) | 与鸡胸肌重和胸肌率相关的snp分子标记及其应用 | |
CN105002267A (zh) | 一种日本对虾分子标记方法及应用 | |
Li et al. | Clonal diversity and genealogical relationships of gibel carp in four hatcheries | |
Scharmann et al. | Sex is determined by XY chromosomes across the radiation of dioecious Nepenthes pitcher plants | |
CN103710427B (zh) | 一种鸡基因的单核苷酸多态性、检测方法及其应用 | |
CN113584181B (zh) | 一种与猪剩余采食量相关的snp分子标记及其用途 | |
JP2019041695A (ja) | ニワトリ胸肉の剪断力価に関与する量的形質遺伝子座およびその利用方法 | |
CN102876777B (zh) | 鮸鱼est微卫星标记的特异引物及筛选方法 | |
CN101921848B (zh) | 一种检测黄牛mgat2基因单核苷酸多态性的方法 | |
CN104894289A (zh) | 用于检测鸡生长性状的试剂盒及鸡的分子育种方法 | |
CN113249492B (zh) | 一种评估猪眼肌面积的snp标记及其应用方法 | |
CN110564867B (zh) | 一种秦川牛cfl1基因的snp分子标记及其检测方法 | |
CN112941198B (zh) | 一种检测猪眼肌面积的snp标记及其应用 | |
CN103320429A (zh) | 一种检测秦川牛Wnt7a基因单核苷酸多态性的方法及应用 | |
CN111910009B (zh) | 一种影响鸡法氏囊指数的分子标记及其应用 | |
CN109439771A (zh) | 一种利用微卫星标记鉴定杂交鲷家系的方法 | |
CN102649962B (zh) | 黄牛wnt10b基因的单核苷酸多态性位点及其检测方法 | |
CN108841930B (zh) | 一种大鳞副泥鳅微卫星家系鉴定方法及其应用 | |
KR101989983B1 (ko) | 재래닭 개체 식별용 초위성체 마커 및 이를 이용한 재래닭의 개체 식별 방법 | |
CN106755370B (zh) | 利用pcr-rflp检测绵羊fth-1基因单核苷酸多态性的方法及其应用 | |
CN103805692B (zh) | 一种检测牛pnpla3基因单核苷酸多态性的方法与应用 | |
KR20130050832A (ko) | 토종닭 순계와 실용계의 유전적 특성 및 품종식별력 분석 방법 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
CB03 | Change of inventor or designer information |
Inventor after: Sun Guirong Inventor after: Han Ruili Inventor after: Yan Fengbin Inventor after: Wang Yanbin Inventor after: Li Zhuanjian Inventor after: Jiao Yuping Inventor after: Wang Shunhong Inventor after: Kang Xiangtao Inventor after: Li Hong Inventor after: Liu Xiaojun Inventor after: Jiang Ruirui Inventor after: Tian Yadong Inventor after: Li Guoxi Inventor before: Sun Guirong Inventor before: Yan Fengbin Inventor before: Wang Yanbin Inventor before: Li Zhuanjian Inventor before: Wang Shunhong Inventor before: Kang Xiangtao Inventor before: Li Hong Inventor before: Liu Xiaojun Inventor before: Jiang Ruirui Inventor before: Tian Yadong Inventor before: Li Guoxi Inventor before: Han Ruili |
|
COR | Change of bibliographic data | ||
CF01 | Termination of patent right due to non-payment of annual fee | ||
CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20150617 Termination date: 20160927 |