CN112941198B - 一种检测猪眼肌面积的snp标记及其应用 - Google Patents
一种检测猪眼肌面积的snp标记及其应用 Download PDFInfo
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- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
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CN116287281B (zh) * | 2022-08-10 | 2024-03-15 | 武汉中粮肉食品有限公司 | 一种与长白猪眼肌面积及瘦肉率性状相关的snp分子标记及其应用 |
CN115820875B (zh) * | 2022-10-24 | 2023-07-25 | 佛山科学技术学院 | 一种用于评估猪眼肌面积的分子标记及其筛选方法和应用 |
Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104561306A (zh) * | 2014-12-31 | 2015-04-29 | 中国农业大学 | 一种与猪眼肌面积性状有关的猪unc13b基因snp标志物 |
CN105331703A (zh) * | 2015-11-16 | 2016-02-17 | 中国农业科学院北京畜牧兽医研究所 | 一种鉴定或辅助鉴定猪100kg体重眼肌面积的方法及其专用试剂盒 |
CN108728552A (zh) * | 2018-04-16 | 2018-11-02 | 华南农业大学 | 一种影响杜洛克猪眼肌面积性状的分子标记及应用 |
KR20190045958A (ko) * | 2017-10-24 | 2019-05-07 | 주식회사 정피엔씨연구소 | 돼지의 고급육 선발을 위한 등지방두께 판별용 단일염기다형성 마커 및 이의용도 |
CN110157818A (zh) * | 2019-07-08 | 2019-08-23 | 云南东恒经贸集团猪育种有限公司 | 一种与猪眼肌高产性状相关的分子标记及其应用 |
CN110257529A (zh) * | 2019-05-09 | 2019-09-20 | 华南农业大学 | 猪6号染色体上与瘦肉率、眼肌面积、眼肌厚度相关的snp分子标记与应用 |
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2020
- 2020-02-17 CN CN202010097989.8A patent/CN112941198B/zh active Active
Patent Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104561306A (zh) * | 2014-12-31 | 2015-04-29 | 中国农业大学 | 一种与猪眼肌面积性状有关的猪unc13b基因snp标志物 |
CN105331703A (zh) * | 2015-11-16 | 2016-02-17 | 中国农业科学院北京畜牧兽医研究所 | 一种鉴定或辅助鉴定猪100kg体重眼肌面积的方法及其专用试剂盒 |
KR20190045958A (ko) * | 2017-10-24 | 2019-05-07 | 주식회사 정피엔씨연구소 | 돼지의 고급육 선발을 위한 등지방두께 판별용 단일염기다형성 마커 및 이의용도 |
CN108728552A (zh) * | 2018-04-16 | 2018-11-02 | 华南农业大学 | 一种影响杜洛克猪眼肌面积性状的分子标记及应用 |
CN110257529A (zh) * | 2019-05-09 | 2019-09-20 | 华南农业大学 | 猪6号染色体上与瘦肉率、眼肌面积、眼肌厚度相关的snp分子标记与应用 |
CN110157818A (zh) * | 2019-07-08 | 2019-08-23 | 云南东恒经贸集团猪育种有限公司 | 一种与猪眼肌高产性状相关的分子标记及其应用 |
Non-Patent Citations (4)
Title |
---|
Genome-wide detection of genetic markers associated with growth and fatness in four pig populations using four approaches;Guo等;《Genetics Selection evolution》;20170214;第1-11页 * |
null.rs319704174.《Ensembl》.2016, * |
rs319704174;null;《Ensembl》;20160731;第1页rs319704174和SNP侧翼序列 * |
猪DECR1基因SNP与大白猪胴体性状的关联分析;陈思佳等;《猪业科学》;20131225;第106-107页 * |
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