CN103655731A - Natural pharmaceutical composition - Google Patents
Natural pharmaceutical composition Download PDFInfo
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- CN103655731A CN103655731A CN201310664299.6A CN201310664299A CN103655731A CN 103655731 A CN103655731 A CN 103655731A CN 201310664299 A CN201310664299 A CN 201310664299A CN 103655731 A CN103655731 A CN 103655731A
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- fructus piperis
- artemisiae argyi
- piperis longi
- folium artemisiae
- extract
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Abstract
The invention relates to a natural pharmaceutical composition and a preparation method thereof. The natural pharmaceutical composition is composed of 2-6 parts of folium artemisiae argyi/fructus piperis longi water decoction concentrated extract, 2-6 parts of folium artemisiae argyi ethyl acetate extract, 3-9 parts of fructus piperis longi ethanol extract and 3-4 parts of piperine. The composition has curative effect on cancer chemotherapy related diarrhoea.
Description
Technical field
The present invention relates to a kind of natural drug composition, relate more specifically to a kind of natural drug composition being comprised of Folium Artemisiae Argyi Fructus Piperis Longi water boiling concentration extract, Folium Artemisiae Argyi ethyl acetate extract, Fructus Piperis Longi ethanol extraction and piperine, the preparation method of this natural drug composition and this natural drug composition are in the purposes for the preparation of reducing in cancer chemotherapy induced diarrhea medicine.
Background technology
Malignant tumor is current harm humans existence, one of healthy principal disease, and its treatment means comprises operation, chemotherapy, radiotherapy, Chinese medicine, and the effect of chemotherapeutic treatment tumor is particularly outstanding.Chemotherapeutic is cell toxicant antitumor drug, alone or combine with the toxicity such as all can cause that patient suffers from diarrhoea, and after chemotherapy of tumors, diarrhoea is again Chemotherapy-induced Diarrhea.In recent years along with chemotherapeutics emerging and applying as 5-fluorouracil, taxanes, hydroxy camptothecin, irinotecan, xeloda etc., the incidence rate of Chemotherapy-induced Diarrhea improves year by year, if do not carry out active treatment, easily cause patient's dehydration, malnutrition, serum electrolyte disorder, acid base imbalance etc., it is unfavorable greatly not only the rehabilitation of body to be brought, also to CC, bring certain difficulty, therefore effective treatment of Chemotherapy-induced Diarrhea has been become the important component part of tumor patient wholistic therapy.
Chemotherapy-induced Diarrhea is with regard to its clinical manifestation, surely belongs to the Chinese medicine category of " having loose bowels ", and Chinese medicine, for the research of having loose bowels, attracts people's attention, but there is no at present the Chinese patent medicine that antagonism cancer chemotherapy has alleviating diarrhoea effect.Therefore, research and develop a kind of high-efficiency low-toxicity, cheap, the natural Chinese medicine that can be used for of being easy to promote alleviates cancer chemotherapy induced diarrhea becomes clinical problem demanding prompt solution.
Folium Artemisiae Argyi is one of tcm clinical practice common medicine, is the leaf of Compositae sagebruss Artemisiae argyi Led.etVant., in China most areas, has distribution.Folium Artemisiae Argyi nature and flavor are bitter, pungent, warm, return liver,spleen,kidney warp, have the effects such as pain relieving, antiabortive, dysentery relieving, dissipating depression of QI.Fructus Piperis Longi is the green fruit of Piperaceae plant Piper longumI., warming spleen and stomach for dispelling cold, therapeutic method to keep the adverse QI flowing downwards pain relieving.Cure mainly: for coldness and pain in the epigastrium, the disease such as vomit, have loose bowels, the inventor is through research and the experiment of each a large amount of formula proportion, finally obtained optimal drug ratio compositions of the present invention, it is the compositions of novelty, and proved that it has fabulous curative effect and can play the effect that suppresses tumor simultaneously cancer chemotherapy induced diarrhea, has completed the present invention thus.
Summary of the invention
A kind of natural drug composition provided by the invention, consists of in the ratio of 2-6:2-6:3-9:3-4 by weight Folium Artemisiae Argyi Fructus Piperis Longi water boiling concentration extract, Folium Artemisiae Argyi ethyl acetate extract, Fructus Piperis Longi ethanol extraction and piperine.
Above-mentioned natural drug composition, wherein the usage ratio of Folium Artemisiae Argyi Fructus Piperis Longi water boiling concentration extract, Folium Artemisiae Argyi ethyl acetate extract, Fructus Piperis Longi ethanol extraction and piperine is 6:6:9:4.
Wherein, Folium Artemisiae Argyi Fructus Piperis Longi water boiling concentration extract, Folium Artemisiae Argyi ethyl acetate extract, Fructus Piperis Longi ethanol extraction can be by well known to a person skilled in the art the conventional method acquisition of purifying, and the natural drug of the present invention that adopts these compositions that conventional method obtains or the one-tenth that adopts business to buy to be grouped into all can obtain identical curative effect.
Preferably, in above-mentioned natural drug composition, the preparation method of Folium Artemisiae Argyi Fructus Piperis Longi water boiling concentration extract is:
According to proportion compatibility, be 1-6: 1 gets Folium Artemisiae Argyi, Fructus Piperis Longi, distilled water immersion, heating decocts, and silk filters, and medicinal residues are adding distil water again, with method, soaks, decocts, filters, and twice filtrate is mixed, and heating is concentrated, ultrafiltration membrance filter, drying under reduced pressure, obtains.
In above-mentioned natural drug composition, the preparation method of Folium Artemisiae Argyi ethyl acetate extract is:
Folium Artemisiae Argyi is appropriate, and 95% ethanol extraction reclaims solvent and obtains alcohol-extracted extract, and extractum, with after normal hexane extraction, again with ethyl acetate extraction, reclaims the ethyl acetate extract obtaining solvent and get final product.
In above-mentioned natural drug composition, Fructus Piperis Longi ethanol extraction preparation method is:
Get Fructus Piperis Longi, pulverize, sieve, soak with ethanol, crosses leaching clear liquor, and residue continues to soak, and twice filtrate is merged, centrifugal, separation of supernatant, and water bath method is concentrated, and drying under reduced pressure becomes dry extract, pulverizes, and sieves, and obtains.
In above-mentioned natural drug composition, process for preparing piperine is:
Fructus Piperis Longi is pulverized, and soak with ethanol, crosses leaching clear liquor, residue continues to soak, and twice filtrate is merged, and is evaporated to thick, it is standing that concentrated solution lets cool place, filters to obtain crude product, and dry crude product is dissolved in ether, 10%KOH solution washing three times, collect ether layer, boil off ether, gained powder is dissolved in ethanol, activated carbon decolorizing recrystallization, obtains.
The present invention also provides the preferred preparation method of this natural drug composition, comprising:
(1) preparation of Folium Artemisiae Argyi Fructus Piperis Longi water boiling concentration extract
According to proportion compatibility, be 1-6: 1 gets Folium Artemisiae Argyi, Fructus Piperis Longi, distilled water immersion, heating decocts, and silk filters, and medicinal residues are adding distil water again, with method, soaks, decocts, filters, and twice filtrate is mixed, and heating is concentrated, ultrafiltration membrance filter, drying under reduced pressure, obtains.
(2) preparation of Folium Artemisiae Argyi ethyl acetate extract
Folium Artemisiae Argyi is appropriate, and 95% ethanol extraction reclaims solvent and obtains alcohol-extracted extract, and extractum, with after normal hexane extraction, again with ethyl acetate extraction, reclaims the ethyl acetate extract obtaining solvent and get final product.
(3) Fructus Piperis Longi ethanol extraction preparation
Get Fructus Piperis Longi, pulverize, sieve, soak with ethanol, crosses leaching clear liquor, and residue continues to soak, and twice filtrate is merged, centrifugal, separation of supernatant, and water bath method is concentrated, and drying under reduced pressure becomes dry extract, pulverizes, and sieves, and obtains.
(4) piperine preparation
Fructus Piperis Longi is pulverized, and soak with ethanol, crosses leaching clear liquor, residue continues to soak, and twice filtrate is merged, and is evaporated to thick, it is standing that concentrated solution lets cool place, filters to obtain crude product, and dry crude product is dissolved in ether, 10%KOH solution washing three times, collect ether layer, boil off ether, gained powder is dissolved in ethanol, activated carbon decolorizing recrystallization, obtains.
(5) choose in proportion Folium Artemisiae Argyi Fructus Piperis Longi water boiling concentration extract, Folium Artemisiae Argyi ethyl acetate extract, Fructus Piperis Longi ethanol extraction and piperine, evenly mix, obtain.
The preparation method of above-mentioned natural drug composition, also comprises: the product that step (5) is made further makes by conventional pharmacy means capsule or the tablet that contains natural drug composition 100mg or 200mg specification.
Natural drug composition of the present invention is as follows at anti-Chemotherapy-induced Diarrhea beneficial effect:
1, natural drug composition of the present invention, causes the research of diarrhea of mouse model to show at irinotecan: the diarrhoea reaction for irinotecan induced mice has obvious antagonism.
2, the inventor combines Folium Artemisiae Argyi Fructus Piperis Longi water boiling concentration extract, Folium Artemisiae Argyi ethyl acetate extract, Fructus Piperis Longi ethanol extraction and piperine originally, and determine the consumption proportion between these components by great many of experiments, make said composition bring into play good synergism, obtain unexpected effect.Pass through our experimentation repeatedly, ratio between each component of natural drug composition of the present invention has produced unexpected synergism when 2-6:2-6:3-9:3-4, especially especially obvious when the usage ratio of each component is 6:6:9:4, this is that the inventor pays a large amount of creative works and obtains, and those of ordinary skills only cannot simply obtain this ratio range and optimum amount ratio by disclosed content.
3, this natural drug composition Folium Artemisiae Argyi Fructus Piperis Longi water boiling concentration extract, Folium Artemisiae Argyi ethyl acetate extract, Fructus Piperis Longi ethanol extraction and each component of piperine all can obtain by conventional means, component also can be buied by business as piperine, and such compositions itself has the convenience of acquisition, lower-cost effect.This is also the product that has splendid curative effect and higher industrial practicality concurrently that inventor obtains by a large amount of creative work sides.
The specific embodiment
Embodiment
The first step: the preparation of various extracts
(1) preparation of Folium Artemisiae Argyi Fructus Piperis Longi water boiling concentration extract
According to proportion compatibility, be 1-6: 1 gets Folium Artemisiae Argyi, Fructus Piperis Longi, distilled water immersion, heating decocts, and silk filters, and medicinal residues are adding distil water again, with method, soaks, decocts, filters, and twice filtrate is mixed, and heating is concentrated, ultrafiltration membrance filter, drying under reduced pressure, obtains.
(2) preparation of Folium Artemisiae Argyi ethyl acetate extract
Folium Artemisiae Argyi is appropriate, and 95% ethanol extraction reclaims solvent and obtains alcohol-extracted extract, and extractum, with after normal hexane extraction, again with ethyl acetate extraction, reclaims the ethyl acetate extract obtaining solvent and get final product.
(3) Fructus Piperis Longi ethanol extraction preparation
Get Fructus Piperis Longi, pulverize, sieve, soak with ethanol, crosses leaching clear liquor, and residue continues to soak, and twice filtrate is merged, centrifugal, separation of supernatant, and water bath method is concentrated, and drying under reduced pressure becomes dry extract, pulverizes, and sieves, and obtains.
(4) piperine preparation
Fructus Piperis Longi is pulverized, and soak with ethanol, crosses leaching clear liquor, residue continues to soak, and twice filtrate is merged, and is evaporated to thick, it is standing that concentrated solution lets cool place, filters to obtain crude product, and dry crude product is dissolved in ether, 10%KOH solution washing three times, collect ether layer, boil off ether, gained powder is dissolved in ethanol, activated carbon decolorizing recrystallization, obtains.
Second step: the preparation of preparation
(1) preparation of natural drug composition capsule preparations of the present invention
Take respectively Folium Artemisiae Argyi Fructus Piperis Longi water boiling concentration extract 24mg, Folium Artemisiae Argyi ethyl acetate extract 24mg, Fructus Piperis Longi ethanol extraction 36mg and piperine 16mg prepared by above-mentioned steps, take starch 395mg, magnesium stearate 5mg simultaneously, evenly mix, be filled to capsule.
(2) preparation of natural drug composition tablet of the present invention
Take respectively Folium Artemisiae Argyi Fructus Piperis Longi water boiling concentration extract 24mg, Folium Artemisiae Argyi ethyl acetate extract 24mg, Fructus Piperis Longi ethanol extraction 36mg and piperine 16mg prepared by above-mentioned steps, take starch 295mg, lactose 100mg simultaneously, evenly mix, with wet granulation, dry, after granulate, mix with 5mg magnesium stearate, be pressed into tablet.
Drug effect and pharmacology's data:
In following test example, compositions is: the usage ratio of Folium Artemisiae Argyi Fructus Piperis Longi water boiling concentration extract, Folium Artemisiae Argyi ethyl acetate extract, Fructus Piperis Longi ethanol extraction and piperine is 6:6:9:4.Inventor's compositions that the multiple proportioning combination between 2-6:2-6:3-9:3-4 time obtains to above-mentioned each composition usage ratio carried out with on identical experiment, all find that the natural drug composition in this amount ranges can obtain good synergism, can play good effect to cancer chemotherapy induced diarrhea.Below the pharmacodynamics data preferred embodiment obtaining:
Test example 1: the pharmacodynamic study of natural drug composition treatment Chemotherapy-induced Diarrhea effect
1, test material
(1) Folium Artemisiae Argyi, Fructus Piperis Longi, is purchased from medical material market.
(2) mice, male, ad lib drinking-water before experiment.
2, test method:
Mice is divided into 5 groups at random, and 5 every group, A group is model group, and B group is Folium Artemisiae Argyi Fructus Piperis Longi water boiling concentration extract 10g.kg
-1(being equivalent to containing crude drug amount), C group is Folium Artemisiae Argyi ethyl acetate extract 10g.kg
-1(being equivalent to containing crude drug amount), D group Fructus Piperis Longi ethanol extraction mixes 10g.kg with piperine in 9:4 ratio
-1(being equivalent to containing crude drug amount), E group is natural drug composition 10g.kg of the present invention
-1(being equivalent to containing crude drug amount), irinotecan intraperitoneal injection (75mg/kg), every day 1 time, continuous 4 days, copies Chemotherapy-induced Diarrhea model.B, C, D, E group by corresponding extract gavage 8 days, is placed in metabolic cage by mice continuously, spreads white filter paper to observe stool situation, and observe mice crissum situation and Mus tail fecal pollution situation at the bottom of cage, and in conjunction with cotton swab stimulation anus defecation, every day, observed and recorded was 2 times.The scoring of diarrhoea degree standard is as follows: 0 minute: defecate normal or do not have; 1 minute: laxativeness, stool is visible slight wet soft; 2 minutes: moderate diarrhoea, stool wets and not molding.And there is slight crissum painted; 3 minutes, severe diarrhoea, watery stool is also painted with severe crissum.
3, experimental result
With model group comparison, natural drug composition group Incidence of Diarrhea of the present invention obviously alleviates.The results are shown in Table 1.
Table 1 present composition is on mice Chemotherapy-induced Diarrhea incidence rate and scoring impact
Note: a is equivalent to containing crude drug amount.
Claims (5)
1. a natural drug composition that reduces cancer chemotherapy induced diarrhea, it is characterized in that said composition consists of in the ratio of 2-6:2-6:3-9:3-4 by weight Folium Artemisiae Argyi Fructus Piperis Longi water boiling concentration extract, Folium Artemisiae Argyi ethyl acetate extract, Fructus Piperis Longi ethanol extraction and piperine
Wherein the preparation method of Folium Artemisiae Argyi Fructus Piperis Longi water boiling concentration extract is:
According to proportion compatibility, be 1-6: 1 gets Folium Artemisiae Argyi, Fructus Piperis Longi, distilled water immersion, heating decocts, and silk filters, and medicinal residues are adding distil water again, with method, soaks, decocts, filters, and twice filtrate is mixed, and heating is concentrated, ultrafiltration membrance filter, drying under reduced pressure, obtains;
Wherein the preparation method of Folium Artemisiae Argyi ethyl acetate extract is:
Folium Artemisiae Argyi is appropriate, and 95% ethanol extraction reclaims solvent and obtains alcohol-extracted extract, and extractum, with after normal hexane extraction, again with ethyl acetate extraction, reclaims the ethyl acetate extract obtaining solvent and get final product;
Wherein the preparation method of Fructus Piperis Longi ethanol extraction is:
Get Fructus Piperis Longi, pulverize, sieve, soak with ethanol, crosses leaching clear liquor, and residue continues to soak, and twice filtrate is merged, centrifugal, separation of supernatant, and water bath method is concentrated, and drying under reduced pressure becomes dry extract, pulverizes, and sieves, and obtains;
Wherein the preparation method of piperine is:
Fructus Piperis Longi is pulverized, and soak with ethanol, crosses leaching clear liquor, residue continues to soak, and twice filtrate is merged, and is evaporated to thick, it is standing that concentrated solution lets cool place, filters to obtain crude product, and dry crude product is dissolved in ether, 10%KOH solution washing three times, collect ether layer, boil off ether, gained powder is dissolved in ethanol, activated carbon decolorizing recrystallization, obtains.
2. natural drug composition as claimed in claim 1, wherein the usage ratio of Folium Artemisiae Argyi Fructus Piperis Longi water boiling concentration extract, Folium Artemisiae Argyi ethyl acetate extract, Fructus Piperis Longi ethanol extraction and piperine is 6:6:9:4.
3. as the preparation method of the natural drug composition of claim 1 or 2, it is characterized in that the method comprises:
(1) preparation of Folium Artemisiae Argyi Fructus Piperis Longi water boiling concentration extract
According to proportion compatibility, be 1-6: 1 gets Folium Artemisiae Argyi, Fructus Piperis Longi, distilled water immersion, heating decocts, and silk filters, and medicinal residues are adding distil water again, with method, soaks, decocts, filters, and twice filtrate is mixed, and heating is concentrated, ultrafiltration membrance filter, drying under reduced pressure, obtains;
(2) preparation of Folium Artemisiae Argyi ethyl acetate extract
Folium Artemisiae Argyi is appropriate, and 95% ethanol extraction reclaims solvent and obtains alcohol-extracted extract, and extractum, with after normal hexane extraction, again with ethyl acetate extraction, reclaims the ethyl acetate extract obtaining solvent and get final product;
(3) preparation of Fructus Piperis Longi ethanol extraction
Get Fructus Piperis Longi, pulverize, sieve, soak with ethanol, crosses leaching clear liquor, and residue continues to soak, and twice filtrate is merged, centrifugal, separation of supernatant, and water bath method is concentrated, and drying under reduced pressure becomes dry extract, pulverizes, and sieves, and obtains;
(4) preparation of piperine
Fructus Piperis Longi is pulverized, and soak with ethanol, crosses leaching clear liquor, residue continues to soak, and twice filtrate is merged, and is evaporated to thick, it is standing that concentrated solution lets cool place, filters to obtain crude product, and dry crude product is dissolved in ether, 10%KOH solution washing three times, collect ether layer, boil off ether, gained powder is dissolved in ethanol, activated carbon decolorizing recrystallization, obtains;
(5) choose in proportion Folium Artemisiae Argyi Fructus Piperis Longi water boiling concentration extract, Folium Artemisiae Argyi ethyl acetate extract, Fructus Piperis Longi ethanol extraction and evenly mix with piperine, obtain.
4. the preparation method of natural drug composition as claimed in claim 3, also comprises: the product that step (5) is made further makes by conventional pharmacy means capsule or the tablet that contains natural drug composition 100mg or 200mg specification.
5. the natural drug composition as described in claim 1-2 any one reduces the purposes in cancer chemotherapy induced diarrhea medicine in preparation.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107280008A (en) * | 2017-07-03 | 2017-10-24 | 阳光医疗运营有限公司 | A kind of dietary supplements and its preparation method and application |
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| CN107280008A (en) * | 2017-07-03 | 2017-10-24 | 阳光医疗运营有限公司 | A kind of dietary supplements and its preparation method and application |
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Application publication date: 20140326 |